KR20170138769A - Artificial breeding method of Locusta migratoria - Google Patents

Abstract

The present invention relates to an artificial Locusta migratoria breeding method. According to the present invention, the artificial Locusta migratoria breeding method includes a process of breeding the nymphs or adults of Locusta migratoria under the conditions including the temperature of 28-30C, humidity of 60-65%, and optical period of 9L : 15D to 12L : 12D. A year-round Locusta migratoria breeding system can be established by providing the artificial Locusta migratoria breeding method, thereby building a standardized breeding technology system for easily breeding the Locusta migratoria in farms as well as supporting an indoor Locusta migratoria breeding technology to contribute to increasing farm household incomes.

Description

{Artificial breeding method of Locusta migratoria}

The present invention relates to a method for artificial rearing of a fulcrum, and more particularly, to a method for artificial rearing of a fulcrum by means of artificial rearing of the fulcrum under specific environmental conditions.

Countries around the world are carrying out national management of biological resources through national key management institutions, and insects are one of the future alternative foods recommended by the United Nations.

Insects are proteins that are more than 50% of body weight, and are rich in minerals and amino acids, and are used for food, livestock, poultry, fish, and pets.

Among them, Locusta migratoria ) is a locust group of locusts, usually 4 ~ 6cm in size, and belongs to large insects, which is already known as an edible insect resource which can be consumed by human as protein source or used for animal feed. Nonetheless, fumigatus is a locust and insect causing crop damage. In recent years, the use of pesticides and insecticides has reduced the incidence. Therefore, the farmers who intend to cultivate them artificially are increasing, but in Korea, ecological studies on the fumigation are insufficient.

Therefore, in order to utilize the fumigation as a medicinal material, research on breeding techniques as well as ecological research should be conducted. Accordingly, the inventors of the present invention completed the present invention by confirming the feed, temperature, humidity, and the like required for optimal feeding of the follicles, while developing an artificial breeding method capable of breeding follicles in large quantities throughout the year so as to utilize the follicles as edible or medicinal products .

1. Korean Patent No. 10-1333773 (Name of the invention: Method of Artificial Breeding of Applied Art, Applicant: Yoon, Chul Ho, Registered Date: November 21, 2013)

1. Research on insect breeding standards and specifications, National Academy of Agricultural Science, 2015. 2. Agricultural Genetic Resources Information Center / Fumigation (http://www.genebank.go.kr/PB/jw/insectView.bo?use_kind=03&species_id=ZE1AA0052)

It is an object of the present invention to provide a method for artificial breeding for massive breeding of females throughout the year.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the particular embodiments that are described. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not restrictive of the invention, There will be.

In order to accomplish the above object, the present invention provides a method for artificial rearing of a fulvicum, which comprises raising a fowl or an adult of a fulvicum under the conditions of a temperature of 28 to 30 DEG C, a humidity of 60 to 65%, a photoperiod of 9L: 15D to 12L: Preferably, the temperature is 30 DEG C, the humidity is 65%, and the light period is 9L: 15D.

Further, it is characterized in that wheat is fed by feeding when nymphs or adults of the fulvicum are raised.

According to another method of artificial rearing of a fulcrum according to the present invention, male and female mating of fulvic adult is characterized by being carried out under the conditions of a temperature of 28 to 30 DEG C, a humidity of 60 to 65% and a light period of 9L: 15D to 12L: 12D , The temperature is 30 DEG C, the humidity is 65%, and the light period is 9L: 15D.

According to another method of artificial rearing of a fulcrum according to the present invention, egg hatching of the fulcrum is performed under the conditions of a temperature of 28 to 30 DEG C, a humidity of 60 to 65%, a light period of 12 L: 12D to 15L: 9D, The temperature is 30 DEG C, the humidity is 65%, and the light period is 15L: 9D.

In addition, when hatching eggshells of the follicles, the food is planted and hatched in a port in which the matsato is laid, and the humidity of the matsato is 40 to 45%.

According to the present invention, as a method of artificial rearing of the fulvum is provided, a standardized rearing technology system that can easily be raised in the farmhouse is established by establishing a fulvial fulvial system during the year, and contributes to the improvement of the farm household income .

Fig. 1 is a graph showing the degree of development of the bollworm by the breeding temperature.
FIG. 2 is a graph showing the growth size of fulvic adult male adults raised at 30 ° C. FIG.
FIG. 3 is a graph showing the degree of development of the bumblebee at 30 ° C.
Fig. 4 is a view showing the time of maturation of the bumblebee by the light period. Fig.
FIG. 5 is a view showing a mating scene of the female adult. FIG.
6 is a view showing the appearance of a bullous nugget and a scattered nugget in a photo.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail, and a detailed description of known functions and configurations that may unnecessarily obscure the gist of the present invention will be omitted.

The present invention relates to a method of artificial rearing of a fulminant fish, which enables a fullerene to be generated and maintained at a desired time in a farmhouse at any time during the year.

For this purpose, the temperature of 28 ~ 30 ℃, the humidity 60 ~ 65%, the light period 9L: 15D ~ 12L: 12D, the feeding condition and the hatching condition of the fulvic nematode ℃, humidity 60 ~ 65%, photoperiod 12L: 12D ~ 15L: 9D, it is important to breed using soil application with Masato.

Specifically, in order to confirm the condition of artificial large breeding during the year, the first generations of the fowl were tested at 25 ° C and 30 ° C, and at the second generation, at 27 ° C and 30 ° C, , The breeding period of the bollworm at 30 ℃ was shorter than the bollworm breeding at 27 ℃ and the breeding period was shorter than that of the bollworm at 30 ℃. It is possible to mass produce a large amount of eggs by spawning. As a result, the temperature of the fowl is raised to 28 ~ 30 ° C, and it is best to breed at 30 ° C.

In other words, when breeding and mating below 28 ° C, as shown in the following experimental results, the developmental characteristics of the bollworm are somewhat low, or especially in the first generation, they do not grow at over 5 years old, The development period is also somewhat long, which causes problems in mass production. If the temperature exceeds 30 ° C., the temperature is too high, which may adversely affect the growth and mating of the bumblebee.

Humidity of 60% to 65% or more than 65% is suitable for breeding and mating of the bumblebee, and there is a risk that the humidity is not matched and the bumblebee is killed or mated in a large amount .

The optimum photoperiod is 9L: 15D ~ 12L: 12D, and when the photoperiod is less than 9L, the light irradiation is insufficient in the fumigation period, and the fertilization process of the fumigation tree is affected. If it is more than 12L, there is a problem that light irradiation is excessively excessive, adversely affecting the growth of the fullerene, and the scattering is not good enough.

However, when the hatching of the fumigation is carried out, the optimum photoperiod is 12L: 12D to 15L: 9D. When the photoperiod is less than 12L, the photoperiod is too short for the hatching of the fumigation egg, There is a problem that the follicle eggs do not hatch excessively.

As a result of investigating the development period and characteristics of the bollworm (wheat and corn) by the type of food (wheat and corn), it was possible to breed wheat in the case of nymphs, but the wheat was not well developed and the wheat was good for the nymphs. As a result of investigating the feeding of adults by feeding amount, corn was also possible but wheat was better.

In addition, the scattering characteristics of fumaracean adults by soil type were found to be 299.3 minutes (about 5 hours) (n = 10), and the number of days from mating to spawning was 3.5 days (n = 14) (N = 14), 1 (n = 14), and n = 13 (n = 13), respectively. ), Body weight, 0.711 + 0.220g; Length, 40.51 ± 10.09 mm; (N = 14), and scattering distance (depth) of 4.6cm (n = 14) in the photoperiod. Wheat and corn) were planted. The hatching rate of fumigation eggs (30 ℃, soil humidity 42%) by soil type was 82.6% for Masato and 63% for soil. .

In addition, it is best to breed in Masato with a humidity of 40 to 45% when the hatching egg is hatching, because when the soil humidity exceeds 45%, for example, 50% to be.

In conclusion, breeding and mating conditions of ferns and adults are 28 ~ 30 ℃, humidity 60 ~ 65%, light period 9L / 15D ~ 12L / 12D, The temperature of 28 ~ 30 ℃, humidity 60 ~ 65%, photoperiod 12L / 12D ~ 15L / 9D, and humidity 40 ~ 45% It can be seen that hatching egg eggs that have been harvested using the photos containing Masato can raise the eggs throughout the year without dormancy at high hatching rates.

Hereinafter, the present invention will be described in more detail with reference to examples, but these examples are for illustrative purposes only and are not intended to limit the scope of protection of the present invention.

< Example  1. Bullwhip by temperature Nymph By gender  Developmental characterization>

1. Experimental Method

The growth period and developmental characteristics (body weight, body length, body width, width) were examined by temperature at 25 ℃ and 30 ℃, respectively. In addition, the growth temperature and growth characteristics (body weight, body length, body width, width) were determined by temperature at 27 ℃ and 30 ℃, respectively.

The breeding conditions were as follows.

- Photoperiod: 15L / 9D

- Illumination: 1,000 lux

- Feed: Wheat and corn (3 cm 20 cm × 20 cm × 30 cm cage with wire netting, put a photo of wheat and corn planted,

- Breeding humidity: 65%

2. Experimental results

As a result of examining the development period and developmental characteristics of the bollworm by the breeding temperature, the development period and developmental characteristics per population (n) were calculated as mean values, respectively, as shown in Tables 1 to 4 and FIG.

령
(First generation of Haenam) 25 ℃ 30 ℃ n
(Population) Development period (days) n
(Population) Development period (days) 1st generation 19 11.3 ± 1.8 34 5.8 ± 2 Second generation 12 8.2 ± 2.0 26 5 ± 1.2 Third generation 4 9.5 ± 3.8 23 4.3 ± 1.3 Fourth 2 6.0 ± 3.0 16 5.7 ± 2.2 5th One 6.0 13 5.4 ± 3.3 6th - - 11 5.7 ± 2.4 Total louse period 41 (up to five) 31.9 days (20.8 days to 5th instar)

령
(Second generation of Haenam) 27 ℃ 30 ℃ n Development period (days) n Development period (days) 1st generation 89 5.7 ± 1.1 43 3.9 ± 1.2 Second generation 71 5.2 ± 1.2 36 3.9 ± 1.2 Third generation 61 6.0 ± 1.2 30 4.1 ± 0.7 Fourth 29 6.6 ± 1.4 24 4.7 ± 1.2 5th 9 8.7 ± 1.1 23 6.4 ± 1.4 Total louse period 16.9 ± 8.0 16.0 ± 8.2

Temperature (℃)
- First generation of Haenam 령 Developmental characteristic n weight
(g) n Length
(mm) n Body width
(mm) n Width
(mm) 25 Second generation 19 0.022 0.005 19 9.2 ± 1.3 19 2.4 ± 0.4 19 2.4 ± 0.4 Third generation 12 0.051 ± 0.009 12 10.5 ± 2 12 3.1 ± 2.6 12 2.6 ± 0.5 Fourth 4 0.085 0.024 One 11.2 One 2.8 One 2.8 5th 2 0.102 + 0.038 One 16.6 ± 3.3 One 3.3 One 3.3 6th - - - - - - - - 30 Second generation 34 0.027 0.005 17 9.7 ± 1.6 18 2.5 ± 0.5 18 2.5 ± 0.9 Third generation 26 0.060 0.012 23 12.8 ± 1.5 23 3.1 ± 0.6 23 3.1 ± 2.6 Fourth 23 0.161 + 0.178 23 15.0 ± 2.4 23 3.6 ± 0.6 23 3.0 ± 0.5 5th 16 0.238 + 0.055 16 18.5 16 3.8 ± 0.7 16 3.3 ± 0.7 6th 13 0.411 + 0.163 12 27.8 ± 4.6 12 6.2 ± 0.9 12 5.0 ± 0.5

* Hatching first instar (n = 160): body weight 0.012 g; Body length 5.9 mm, body width 1.8 mm; Width 1.6mm

Temperature (℃)
- Second generation of Haenam 령 Developmental characteristic n weight
(g) n Length
(mm) n Body width
(mm) n Width
(mm) 27 1st generation 89 0.011 ± 0.002 76 6.2 ± 0.5 76 1.7 ± 0.1 76 1.8 ± 0.1 Second generation 77 0.027 0.010 69 9.3 ± 0.6 69 2.6 ± 0.2 69 2.6 ± 0.2 Third generation 66 0.062 0.010 64 13.9 ± 1.2 64 3.6 ± 0.3 64 3.6 ± 0.3 Fourth 55 0.148 ± 0.058 53 19.3 ± 1.9 53 3.0 ± 0.6 53 4.9 ± 0.5 5th 27 0.327 ± 0.057 26 27.3 ± 2.6 26 6.7 ± 0.7 26 6.7 ± 0.7 30 1st generation 43 0.011 ± 0.002 32 6.3 ± 0.5 33 1.8 ± 0.1 33 1.8 ± 0.1 Second generation 38 0.027 0.005 37 9.9 ± 0.5 37 2.6 ± 0.2 37 2.6 ± 0.2 Third generation 32 0.068 ± 0.003 16 14.6 ± 0.9 16 3.6 ± 02 16 3.6 ± 0.3 Fourth 26 0.148 + 0.028 24 20.3 ± 3.2 24 4.9 ± 0.5 24 5.0 ± 0.6 5th 26 0.363 + 0.097 20 29.7 ± 3.9 19 6.9 ± 0.9 19 6.9 ± 0.9

* Hatching first instar (n = 160): body weight 0.012 g; Body length 5.9 mm, body width 1.8 mm; Width 1.6mm

As shown in FIG. 1 and Tables 1 to 4, it can be seen that the suitable temperature for the artificial mass proliferation of the wilted buds is 28 to 30 ° C.

In other words, when the bollworm was raised at 30 ℃, the first generation of the nymphs was up to 6 days old and the development period of the nymphs was about 31.9 days. In case of breeding at 25 ℃, The period of time is about 41 days. When it grows at 25 ℃, the development of the white worms slows down, and it takes a long time to grow up to the 5th instar, and the growth stops at the 5th instar, so the adult does not develop. In addition, breeding characteristics such as body weight, body length, body width, and width of bumblebee of Bumblebee are better than those of Bumblebee at 30 ℃, so that when bumblebee is bred at 30 ℃, It is possible to breed a fulvrum showing excellent growth characteristics.

In addition, although the second-generation bumblebee was identified by temperature, the development period of the second-generation nymph was about 16 days at 27 ° C and about 17 days at 27 ° C, In the second generation of the nymphs, the development period is shorter than 30 ℃. In addition, the body weight, body length, body width, and width of the second generation bumblebee were confirmed, and this was also superior to the first generation bumblebee at 30 ° C. As a result, when the bumblebee was raised, It is preferable that the breeding is carried out at 28 to 30 DEG C in all the generations exceeding 27 DEG C, and it is most preferable to breed at 30 DEG C.

< Example  2. Identification of developmental characteristics of adult white wolves by breeding temperature>

1. Experimental Method

This study was conducted to investigate the adult size of adult female woolen moths by applying the rearing conditions of the female moths.

The breeding conditions were as follows.

- Temperature: 30 ℃

- Photoperiod: 15L / 9D

- Illumination: 1,000 lux

- Feed: Wheat and corn (3 cm 20 cm × 20 cm × 30 cm cage with wire netting, put the photo with wheat and corn planted and let the adults eat it)

- Breeding humidity: 65%

2. Experimental results

As a result of the above experiment, the developmental characteristics per population (n) were calculated as average values, and the results were shown in Table 5 and FIG.

Male and female Developmental characteristic n Fable days
(Work) n weight
(g) n Length
(mm) n Body width
(mm) n Width
(mm) cancer 10 30.6 ± 1.8 11 0.82 + 0.21 11 41.7 ± 5.6 11 9.3 ± 1.1 11 6.8 ± 0.9 Number 11 31.9 ± 3.1 12 0.62 + 0.11 12 35.9 ± 4.0 12 7.7 ± 0.8 12 5.6 ± 0.6

As shown in Table 5 and FIG. 2, in females raised at 30 ° C., females were larger than males and females were also found to be faster than females. As females were faster in number of females, It is possible to breed mass by making more.

< Example  3. Guangzhou Message  Wool Nymph  Developmental characterization>

1. Experimental Method

The growth period and developmental characteristics (weight, body length, body width, width) of the bollworms were examined by Gwangju with 9L / 15D and 15L / 9D as photoperiod conditions.

The breeding conditions were as follows.

- Temperature: 30 ℃

- Illumination: 1,000 lux

- Feed: Wheat and corn (3 cm 20 cm × 20 cm × 30 cm cage with wire netting, put a photo of wheat and corn planted,

- Breeding humidity: 65%

2. Experimental results

As a result of the developmental period and developmental characteristics of the bollworm according to the breeding period, the development period and the developmental characteristics per population (n) were calculated as average values, respectively, as shown in Tables 6 and 7 below.

령 9L / 15D 15L / 9D n Development period (days) n Development period (days) 1st generation 28 5.8 ± 2.0 34 7.5 ± 2.8 Second generation 20 5.0 ± 0.9 26 5.0 ± 1.2 Third generation 19 3.5 ± 1.4 23 4.3 ± 1.3 Fourth 18 4.1 ± 1.8 16 5.7 ± 2.2 5th 14 5.4 ± 3.4 13 7.9 ± 2.8 6th 12 4.4 ± 3.1 11 5.7 ± 2.4 Total louse period 31.9 32.4

Guangzhou 령 Developmental characteristic n weight
(g) n Length
(mm) n Body width
(mm) n Width
(mm) 9L /
15D Second generation 27 0.035 0.012 18 12.4 ± 2.7 18 3.1 ± 0.7 18 2.9 ± 0.7 Third generation 19 0.074 0.018 17 13.9 ± 1.4 17 3.5 ± 0.5 17 3 ± 0.4 Fourth 18 0.161 + 0.178 18 15 ± 2.4 18 3.7 ± 0.3 18 3 ± 0.5 5th 18 0.238 + 0.055 17 19.1 ± 4.5 17 4.7 ± 0.9 17 4.1 ± 0.8 6th 14 0.608 ± 0.058 12 30.5 ± 5.4 12 6.6 ± 1.5 12 5.2 ± 0.8 15L /
9D Second generation 34 0.027 0.005 17 9.7 ± 1.6 18 2.5 ± 0.5 18 2.5 ± 0.9 Third generation 26 0.06 ± 0.012 23 12.8 ± 1.5 23 3.1 ± 0.6 23 2.6 ± 0.5 Fourth 23 0.127 + 0.04 23 14.8 ± 1.5 23 3.6 ± 0.6 23 2.9 ± 0.3 5th 16 0.228 ± 0.058 16 16.6 ± 3.3 16 3.8 ± 0.7 16 3.3 ± 0.7 6th 13 0.411 + 0.163 12 27.8 ± 4.6 12 6.2 ± 0.9 12 5 ± 0.5

* Hatching first instar (n = 160): body weight 0.012 g; Body length 5.9 mm, body width 1.8 mm; Width 1.6mm

As shown in FIG. 3 and Tables 6 and 7, it can be seen that the photoperiod suitable for the artificial mass proliferation of the boll weevil is 9L / 15D.

In other words, when the bumblebee was raised, the period of the bumblebug period was about 32.4 days at 15L / 9D, while the period of the bumblebug period was about 31.9 days at 9L / 15D, , And the developmental characteristics such as body weight, body length, body width, and width of the worms were the best in the case of 9L / 15D, and 9L / 15D in the case of bollworm breeding It can be seen that it is suitable.

< Example  4. Guangzhou Message  Identification of Developmental Characteristics of Fulvic Adult>

1. Experimental Method

The developmental characteristics, mating characteristics, egg size, egg hatching rate and number of hatching days of female adult woolen moths were investigated by applying the rearing conditions of the woolen moths.

The breeding conditions were as follows.

- Temperature: 30 ℃

- Photoperiod: 15L / 9D, 9L / 15D

- Illumination: 1,000 lux

- Feed: Wheat and corn (3 cm 20 cm × 20 cm × 30 cm cage with wire netting, put the photo with wheat and corn planted and let the adults eat it)

- Breeding humidity: 65%

2. Experimental results

1) Developmental characteristics

As a result of the above experiment, the developmental characteristics of male and female adult bulb per male population (n) were calculated as average values, respectively.

Guangzhou Male and female Developmental characteristic n Fable days
(Work) n weight
(g) n Length
(mm) n Body width
(mm) n Width
(mm) 9L / 15D cancer 6 30.7 ± 2.1 6 0.89 ± 0.16 6 42.5 ± 7.9 6 10.0 ± 1.3 6 7.1 ± 1.1 Number 8 32.4 ± 3.7 8 0.63 + 0.13 8 37.8 ± 2.2 8 7.8 ± 0.5 8 5.7 ± 0.3 15L / 9D cancer 6 29.5 ± 3.5 6 0.77 + - 0.23 6 42.1 ± 2.5 6 9.4 ± 1.5 6 7.1 ± 1.2 Number 5 30.4 ± 2.1 5 0.60 + - 0.10 5 32.9 ± 3.9 5 7.6 ± 1.3 5 5.4 ± 0.8

As shown in the above Table 8, when the photoperiod is irradiated with the 9L / 15D photoperiod, the number of days of longevity is shorter than that of 15L / 9D, and the developmental characteristics such as body weight, , It can be seen that the photoperiod of 9L / 15D is suitable for the cultivation of the female bulb.

<Example 5> Identification of developmental characteristics of the worms by the type of food>

1. Experimental Method

The developmental characteristics and developmental characteristics (weight, body length, body width, width) of the bollworms of each species were examined using wheat and corn.

The breeding conditions were as follows.

- Temperature: 30 ℃

- Illumination: 1,000 lux

- Photoperiod: 9L / 15D

- Breeding humidity: 65%

2. Experimental results

As a result of the developmental period and developmental characteristics of the bollworms of each breeding food, the development period and developmental characteristics per population (n) were calculated as average values respectively, as shown in Tables 9 and 10 below.

령 wheat corn n Development period (days) n Development period (days) 1st generation 76 7.4 ± 2.9 5 12.2 ± 3.5 Second generation 57 4.0 2 5.7 ± 1.8 Third generation 45 4.4 ± 2.4 One 6.0 Fourth 36 4.8 ± 2.2 One 8.0 5th 28 6.0 One 6.7 ± 3.3 6th 24 5.0 ± 2.9 One 7.0 Total louse period 31.2 34

food 령 n weight n Length n Body width n Width wheat Second generation 76 0.029 ± 0.01 38 10.9 ± 2.6 38 2.8 ± 0.6 38 2.6 ± 0.6 Third generation 57 0.063 0.016 50 13 50 3.2 ± 1.4 50 2.6 ± 0.5 Fourth 45 0.141 + 0.132 42 14.8 ± 2.1 42 4.1 42 3.6 5th 35 0.333 33 24.3 33 6.6 33 5.4 6th 27 0.513 + 0.271 24 33.8 24 7.1 24 5.5 corn Second generation 4 0.024 ± 0.007 4 8.5 ± 1.5 4 2.4 ± 0.4 4 2.3 ± 0.4 Third generation One 0.058 One 12.2 ± 2.7 One 3.1 One 2.6 Fourth One 0.14 One 14.5 One 3.6 ± 0.5 One 3 ± 0.4 5th One 0.224 + 0.058 One 17.7 ± 4 One 4.2 ± 0.8 One 3.7 ± 0.8 6th One 0.5 One 29 ± 5.1 One 6.4 ± 1.2 One 5.1 ± 0.7

* Hatching first instar (n = 160): body weight 0.012 g; Body length 5.9 mm, body width 1.8 mm; Width 1.6mm

As shown in Tables 9 and 10, it can be seen that the feed suitable for the artificial mass proliferation of the bollworm nematodes is 'wheat'.

In other words, when bollworms were raised, wheat was fed to feeds fed at the same temperature and in the light of the normal bollworm, when the bollworms were fed, the development period of the bollworm was shorter than that of raising corn And the growth characteristics such as body weight, body length, body width, and width of the worms of the worms are more excellent than those of feeding corn when they are fed with wheat, It is suitable to breed.

< Example  6. By the naked eye, Amount of food  Check>

1. Experimental Method

Put a photo of wheat (11 to 12) and corn (11 to 12) in a single breeding box (20 cm × 20 cm × 30 cm made of wire mesh) and put in a pair of male and female to add wheat and corn The amount of food consumed was counted visually. The above procedure was repeated for two times in total.

2. Experimental results

As a result of the above experiment, the amount of food per population (n) was calculated as an average value as shown in Table 11 below.

repeat wheat corn n Feed amount (%) n Feed amount (%) One 11 61.5 11 38.5 2 12 53.5 12 46.5 Average 57.5 42.5

As shown in the above Table 11, it was confirmed that when the wheat was fed more than corn as in the case of the above-mentioned fungus, the amount of fungus consumed by the fungus was more than that of the fungus, .

< Example  7. Confirmation of mating characteristics of adult white walnut>

1. Experimental Method

The mating characteristics of the females of Fulvicum were identified from the Gwangju area (fable - mating days, mating time, mating - spawning days, nematodes).

At this time, the breeding condition at mating is as follows.

- Temperature: 30 ℃

- Illumination: 1,000 lux

- Optical instruments: 9L / 15D, 15D / 9L

- Breeding humidity: 65%

2. Experimental results

Table 13 (mating time), Table 14 (mating-scattering days, total number of marsupials), Table 15 and Fig. 4 (mating time zone) .

Guangzhou
Number of matings 1 time Episode 2 3rd time 4 times 5 times 6 times 7 times 8 times 9 times 10 times 11 times 12 times 13 times 9L / 15D Average 7.8
±
2.5
(5) 12.6 ±
2.2
(5) 17.4 ±
3.3
(5) 17.8 ±
1.5
(5) 20.5 ±
1.7
(4) 25.3 ±
2.5
(4) 29.0 ±
1.7
(3) 38.7 ±
4.2
(3) 43.7 ±
8.0
(3) range 6- 11 10-16 15-
23 16-19 18-22 22-28 27-
30 34-
42 36-
52 ratio
(%) 13.9 13.9 13.9 11.1 11.1 11.1 8.3 8.3 8.3 15L / 9D Average 14.8 ±
3.8
(5) 17.8 ±
3.8
(5) 20.0 ±
3.9
(5) 23.6 ±
6.1
(5) 25.8 ±
5.4
(4) 27.8 ±
5.9
(4) 30.5 ±
6.0
(4) 33.5 ±
7.5
(4) 36.8 ±
7.1
(4) 39.5 ±
7.6
(4) 42.5 ±
4.9
(4) 48.0 ±
8.5
(2) 47.0
(One) range 12-19 11-20 13-
22 15-32 20-33 21-35 23-
37 24-
40 27-
41 30-
47 37-
48 42-
54 47 ratio
(%) 9.8 9.8 9.8 9.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 3.9 2.0

Guangzhou
Mating time (min) 1 time Episode 2 3rd time 4 times 5 times 6 times 7 times 8 times 9 times 10 times 11 times 12 times 13 times 9L / 15D Average 128.8
±
67.8
(5) 250.6 ±
91.2
(5) 169.6 ±
93.5
(5) 279.3 ±
128.5
(4 187.0
±
67.4
(4) 277.5 ±
113.2
(4) 173.3
±
50.3
(3) 344.0 ±
118.8
(2) 450
(One) range 70- 244 120-363 80-
270 157-
460 105-
270 120-
350 120-
220 260-
428 450 15L / 9D Average 124.0
±
71.3
(5) 147.0
±
100.7
(5) 128.0
±
61.0
(4) 132.8
±
71.8
(4) 185.5
±
38.5
(4) 236.8
±
77.6
(4) 176.3
±
92.9
(4) 172.5
±
78.9
(4) 321.3
±
131.2
(3) 219.3
±
196.8
(3) 195.7
±
94.2
(3) 423.5
±
108.2
(2) 410
(One) range 50- 240 60- 290 60-192 90- 240 150- 240 140- 330 50- 269 60- 244 170- 404 54-437 90- 271 347-500 410

Guangzhou Mating - Days to laying
(Work) I'm a monster
(Dogs / horses) 9L / 15D 2.0 ± 0.0
(3) 2.2
(10) 15L / 9D 2.5 ± 0.8
(5) 1.9
(14)

n 9-10 10-11 o'clock 11-12 12-13 o'clock 13-14 o'clock 14-15 15-16 o'clock 16-17 o'clock 17-18 9L / 15D 29 34.5 27.6 10.3 0 6.9 10.3 6.9 3.4 15L / 9D 61 39.3 13.1 1.6 1.6 8.2 16.4 11.5 6.6 1.6

As shown in Table 13 (mating time), mating time of mating female adults is 124.8-450 min (n = 10), and in particular, as shown in Table 15 and FIG. 4, the most active As shown in FIG. 5, it was confirmed that the male was on the female's back and the end of the abdomen was put up above the female abdomen to mate with each other. Since the mating characteristics of such a female was not known to date, It can be applied as a standard system of the method.

(N = 5) at 9L / 15D and 14.8 ± 3.8 days (n = 5) at 15L / 9D, as shown in Table 12 (allegorical-mating days) 5). As shown in Table 14 (mating-spawning days, necropsy), the number of mating-mating days and mating time in the treatment group irradiated with 15L / 9D was larger than that in the group treated with 9L / 15D. The number of days to mating - spawning was also similar in 2 days (n = 3) from 9L / 15D and 2.5 days (n = 3) in 15L / 9D, whereas 2.2L in 9L / 15D and 15L / 9D And 1.9 eggs / day, respectively. It can be seen that 9L / 15D is most suitable for mating male and female adults. In other words, in the treatment group with 15L / 9D, the total number of matings was large, but the number of matings was small compared to the number of matings. On the other hand, in the group treated with 9L / 15D, the total mating frequency was small, It is found that the long-term L survey is adversely affecting the spawning.

< Example  8. Identification of Scattering Characteristics of Bullworm Adults by Soil Type>

1. Experimental Method

And the soil type was classified into horticultural soil and marathon, and the scattering characteristics (egg size, spawning - hatching period, hatching rate, scattering site) of the fulvic adult were confirmed.

The breeding conditions were as follows.

- Temperature: 30 ℃

- Illumination: 1,000 lux

- Photoperiod: 15L / 9D, 9L / 15D

- Breeding humidity: 65%

- Soil humidity: 42%

2. Experimental results

The scattering characteristics of the fulvulus adults according to the soil were investigated as shown in Tables 16, 17, 18 and 6 below.

Spawning place Photo Horticultural soil Masato wheat corn Average 50.0% 42.9% 0.0% 7.1%

Spawning place Hatching rate (%) n Horticultural soil n Masato Average 5 63 6 82.6

Guangzhou I'm a monster
(Dogs / horses) Spawning - The period until hatching
(Work) Hatching rate
(%) 9L / 15D 1.2
(5) 14.6 ± 3.1
(5) 79.0
(5) 15L / 9D 1.0
(5) 14.0 ± 2.5
(6) 87.9
(6)

As a result of the experiment, as shown in Table 16, it can be seen that the bulbous adults generally scatter much in the port where the food (wheat or corn) and the soil are contained together rather than simply the soil.

In addition, as shown in Table 17, the hatching rate of fumigation eggs is 82.6% for Masato and 63% for horticultural soil, as shown in Table 17. As shown in Table 17, it is found that application of Masato on fumigation eggs is suitable for fumigation artificial rearing have. In other words, the moisture contained in the soil is lost due to the illumination and light period applied at the time of hatching of the fumigation eggs. At this time, in the case of the soil, the hatching rate is lowered due to insufficient water retention. It is also shown that the application of wheat and masato is most suitable for hatching eggs.

As shown in Table 18, the number of days until spawning-hatching was 14.6 ± 3.1 days (n = 5) at 9L / 15D and 14.0 ± 2.5 days at 15L / 9D (n = 6). However, the hatching rate was 79.0% (n = 5) at 9L / 15D, while 87.9% (n = 6) at 15L / 9D showed the hatching rate was lower at 9L / 15D In the case of bar and egg hatching, it is seen that the photoperiod of 15L / 9D is the most suitable.

In addition, as shown in Fig. 6, the shape of the nugget and the shape of the nugget were found to be scattered in the photo. As shown in Fig. 6, the nugget was 1.14 (n = 14) Size) (n = 13) were weight, 0.711 ± 0.220 g; Length, 40.51 ± 10.09 mm; Width, 8.42 ± 1.55mm, and the scattering distance (depth) in the photo soil was 4.6cm (n = 14).

The scattering characteristics of these bums are not known to date, and this can be applied as a standard system for bumblebeing throughout the year.

In particular, in the case of hatching the follicular egg, it is usually stored at 5 ° C or 7.5 ° C for about 2 months in a refrigerator, and then incubated at 30 ° C for breeding. As a result, the present invention is not limited to the specific Breeding and egg hatching conditions, it is possible to breed bulbworms through the year without dormancy for about 2 months.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined by the appended claims. It will be possible. The scope of the present invention is defined by the appended claims, and all differences within the scope of the claims are to be construed as being included in the present invention.

Claims (9)

In terms of the year-round mass rearing of the fumum,
The fowl of the fulminant moth or the adult is raised under the conditions of a temperature of 28 to 30 DEG C, a humidity of 60 to 65% and a light period 9L: 15D to 12L: 12D.
Artificial Breeding Method of Fulvicum.
The method according to claim 1,
Wherein the temperature is 30 DEG C, the humidity is 65%, and the light period is 9L: 15D.
Artificial Breeding Method of Fulvicum.
The method according to claim 1,
Characterized in that the wheat is fed to the feeder during the raising of the nematode or adults of the fumaricum.
Artificial Breeding Method of Fulvicum.
In terms of the year-round mass rearing of the fumum,
Wherein the male and female matings of the fulvic adult are carried out under the conditions of a temperature of 28 to 30 DEG C, a humidity of 60 to 65% and a light period of 9L: 15D to 12L: 12D.
Artificial Breeding Method of Fulvicum.
5. The method of claim 4,
Wherein the temperature is 30 DEG C, the humidity is 65%, and the light period is 9L: 15D.
Artificial Breeding Method of Fulvicum.
In terms of the year-round mass rearing of the fumum,
Characterized in that the bulging of the bulbum is carried out under the conditions of a temperature of 28 to 30 DEG C, a humidity of 60 to 65% and a light period of 12 L: 12D to 15L: 9D.
Artificial Breeding Method of Fulvicum.
The method according to claim 6,
Wherein the temperature is 30 DEG C, the humidity is 65%, and the light period is 15L: 9D.
Artificial Breeding Method of Fulvicum.
The method according to claim 6,
When hatching egg of the above-mentioned bulbum, it is characterized in that the food is planted and hatched in the port where Masato is laid,
Artificial Breeding Method of Fulvicum.
The method according to claim 6,
Wherein the humidity of the saccade is 40 to 45%.
Artificial Breeding Method of Fulvicum.

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