CN108184773A - Method for improving survival rate of low-altitude bred bat moth larvae - Google Patents
Method for improving survival rate of low-altitude bred bat moth larvae Download PDFInfo
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- CN108184773A CN108184773A CN201810216178.8A CN201810216178A CN108184773A CN 108184773 A CN108184773 A CN 108184773A CN 201810216178 A CN201810216178 A CN 201810216178A CN 108184773 A CN108184773 A CN 108184773A
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- bat moth
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- temperature
- raising
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- 238000000034 method Methods 0.000 title claims abstract description 44
- 230000004083 survival effect Effects 0.000 title claims abstract description 32
- 241000330899 Hepialus Species 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 description 9
- 230000001488 breeding effect Effects 0.000 description 9
- 230000035611 feeding Effects 0.000 description 7
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 5
- 241000288673 Chiroptera Species 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000006266 hibernation Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000009987 spinning Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 235000007519 Chimonanthus praecox Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 240000001359 Origanum dictamnus Species 0.000 description 1
- 235000015987 Origanum dictamnus Nutrition 0.000 description 1
- 235000004383 Origanum vulgare subsp. vulgare Nutrition 0.000 description 1
- 241000764065 Persicaria capitata Species 0.000 description 1
- 235000012804 Polygonum viviparum Nutrition 0.000 description 1
- 244000228295 Polygonum viviparum Species 0.000 description 1
- PJANXHGTPQOBST-VAWYXSNFSA-N Stilbene Natural products C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- RECVMTHOQWMYFX-UHFFFAOYSA-N oxygen(1+) dihydride Chemical compound [OH2+] RECVMTHOQWMYFX-UHFFFAOYSA-N 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000009711 regulatory function Effects 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical compound C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to a method for improving survival rate of bat moth larvae raised at low altitude, which utilizes an intelligent constant temperature and humidity test box to respectively regulate and control different temperatures and humidities at different growth stages of the bat moth larvae in 3-5 instars. The invention has the beneficial effects that: the invention better solves the problem that the hepialus larva loses water in the 3-instar stage to cause the death of the dried larva, improves the physique of the hepialus larva in the 4-instar stage, reduces the infection rate of the feeding environment and effectively improves the survival rate of the hepialus larva in the 5-instar stage.
Description
Technical field
The invention belongs to cordyceps sinensis artificial bionic technical field of cultivation, and in particular to a kind of raising low altitude area raising bat
The method of moth larvae survival rate.
Background technology
The cordyceps sinensis artificial culture most important condition is successfully to raise Hepialus larva under the conditions of natural growing environment is simulated.It posts
Main bat moth completes to need 4-5 growth cycles under natural environmental condition, larva happiness low temperature, humidity, non-refractory, and temperature is higher than
20 DEG C with regard to massive mortality, when temperature is too low spinning build " nest " and keep internal water, soil moisture content is excessive to climb out of ground often,
Keeping afloat can survive a couple of days.Larva mainly gnaws the plant roots such as polygonum capitatum, serpentgrass and Radix et Rhizoma Rhei, also gnaws undershrub shape Huang
Stilbene and golden wintersweet and other plant root system.
The Hepialus of severe cold areas is guided to the laboratory rearing of low altitude area, realizes that host insect indoors artificial culture is
It is sufficiently complex and difficult.Scholars are to the suitable feed, temperature, wet in host of Cordyceps sinensis growth and development process for many years
Degree, illumination, breeding dust etc. have carried out the artificial feeding that numerous studies originate in area's progress in High aititude at present, due to technical conditions
With the influence of traffic geography factor, the control requirement of humiture is extremely difficult to, and can be simulated in low altitude area different warm and humid
Degree condition is so as to meet requirement of the different worm age grade sections to humiture.
Invention content
Of the existing technology in order to solve the problems, such as, the present invention provides a kind of raising low altitude area raising bat moth larvaes to deposit
The method of motility rate has the characteristics that enhance bat moth larvae constitution, improves bat moth larva survival rate.
The object of the present invention is to provide a kind of methods for improving low altitude area raising bat moth survival rate of larvae.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, the method
Include the following steps：
(1) in 10~12 DEG C of temperature, under the conditions of humidity is 70~75RH%, 3 age bat moth larvaes are raised；
(2) in 7~9 DEG C of temperature, under the conditions of humidity is 70~75RH%, 4 age bat moth larvaes are raised；
(3) in 9~12 DEG C of temperature, under the conditions of humidity is 60~65RH%, 5 age bat moth larvaes are raised.
The key of bat moth larva raising is to maintain the eubolism of bat moth larvae, only the moderate temperature in metabolism
Area, bat moth larvae are comfortable on, and just with stronger vitality, the regulatory function of itself could be relied on normally to be grown
Metabolism.And the comfort of polypide is more difficult judgement, while the conditions such as humiture of external environment can not be directly translated into polypide
Comfort, bat moth larvae is primary in high altitude localities in addition, has got used to surviving in the variation in proper order of nature, low
Altitude Regions can not simulating high altitude environment completely, the weather conditions of nature, water and soil condition cannot more be replicated.
3 age of place of production bat moth larvae enters hibernation period, and larva spinning builds " nest " and keeps internal water, and during 3 age of larva of indoor raising
Often drying shrinkage is dead.
The method for breeding of the present invention at the 3 instar larvae phase of bat moth, improves the humiture of raising, improves bat moth larvae
Activity, enhance the food ingestion of larva, reduce larva drying shrinkage death probability, temperature is gradually reduced after 4 initial stages in age, it then follows is natural
The rule of boundary's larva hibernation makes larva make the suspend mode of certain time during low temperature, reduces a large amount of feedings of larva and susceptible several
Rate remakes raising temperature raising processing, gradually enhances larval feeding amount, larva is made to raise item indoors after 5 instar larvae initial stages
Survival rate under part is improved.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, 1,2 age bats
In Hepialus larva feeding process, controlled at 9~10 DEG C, humidity is 75~80RH%.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, step (1)
When being converted to step (2), temperature gradually reduces to 7~9 DEG C.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, step (1)
When being converted to step (2), 1 DEG C is reduced within every 5 days until temperature reaches 7~9 DEG C.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, step (2)
When being converted to step (3), temperature is gradually increased to 9~12 DEG C.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, step (2)
When being converted to step (3), 1 DEG C is increased within every 5 days until temperature reaches 9~12 DEG C.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, 2 age bats
When raising of the raising of moth larvae to 3 age bat moth larvaes converts, temperature is gradually increased to 10~12 DEG C.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, 2 age bats
When raising of the raising of moth larvae to 3 age bat moth larvaes converts, 1 DEG C is increased within every 5 days until temperature reaches 10~12 DEG C.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, in bat moth
In the feeding process of larva, sterile working is kept.
According to the method for improving low altitude area raising bat moth survival rate of larvae of the specific embodiment of the invention, in bat moth
In the feeding process of larva, feed is replaced within every 15~25 days.
Beneficial effects of the present invention are：
The present invention simulates in low altitude area laboratory and originates in area's humiture progress on the spot artificial feeding 3 age Hepialus larva, profit
Regulate and control different humitures respectively in Hepialus larva 3~5 age different stages of growth with intelligent constant-temperature constant moisture test case, it is preferable to solve
Hepialus larva of having determined 3 age grade sections lack moisture, drying shrinkage is dead the problem of；Hepialus larva 4 age constitution is improved, reduces raising
Environmental infection rate；Effectively raise the survival rate of 5 age Hepialus larvas.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme of the present invention will be carried out below
Detailed description.Obviously, described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all under the premise of creative work is not made
Other embodiment belongs to the range that the present invention is protected.
Embodiment 1
The method for breeding of the bat moth larvae of the present invention, includes the following steps：
(1) in 9 DEG C of temperature, under the conditions of humidity is 75RH%, 1,2 age bat moth larvaes are raised；
(2) in 10 DEG C of temperature, under the conditions of humidity is 70RH%, 3 age bat moth larvaes are raised；
(3) in 7 DEG C of temperature, under the conditions of humidity is 70RH%, 4 age bat moth larvaes are raised；
(4) in 9 DEG C of temperature, under the conditions of humidity is 60RH%, 5 age bat moth larvaes are raised.
Embodiment 2
The method for breeding of the bat moth larvae of the present invention, includes the following steps：
(1) in 10 DEG C of temperature, under the conditions of humidity is 78RH%, 1,2 age bat moth larvaes are raised；
(2) in 11 DEG C of temperature, under the conditions of humidity is 73RH%, 3 age bat moth larvaes are raised；
(3) in 8 DEG C of temperature, under the conditions of humidity is 72RH%, 4 age bat moth larvaes are raised；
(4) in 10 DEG C of temperature, under the conditions of humidity is 63RH%, 5 age bat moth larvaes are raised.
Embodiment 3
The method for breeding of the bat moth larvae of the present invention, includes the following steps：
(1) in 10 DEG C of temperature, under the conditions of humidity is 80RH%, 1,2 age bat moth larvaes are raised；
(2) in 12 DEG C of temperature, under the conditions of humidity is 75RH%, 3 age bat moth larvaes are raised；
(3) in 9 DEG C of temperature, under the conditions of humidity is 75RH%, 4 age bat moth larvaes are raised；
(4) in 12 DEG C of temperature, under the conditions of humidity is 65RH%, 5 age bat moth larvaes are raised.
Embodiment 4
The method for breeding of the bat moth larvae of the present invention, includes the following steps：
(1) in 9 DEG C of temperature, under the conditions of humidity is 75RH%, 1,2 age bat moth larvaes are raised；
(2) after temperature first being adjusted to 10 DEG C, 5 days, then temperature is adjusted to 11 DEG C；In 11 DEG C of temperature, humidity is
Under the conditions of 73RH%, 3 age bat moth larvaes are raised；
(3) after temperature first being adjusted to 10 DEG C, 5 days, then temperature is adjusted to 9 DEG C；In 9 DEG C of temperature, humidity 70RH%
Under the conditions of, raise 4 age bat moth larvaes；
(4) after temperature first being raised to 10 DEG C, 5 days by 9 DEG C, then temperature risen to 11 DEG C by 10 DEG C；In 11 DEG C of temperature, humidity
Under the conditions of 65RH%, 5 age bat moth larvaes are raised.
Embodiment 5
The method for breeding of the bat moth larvae of the present invention, includes the following steps：
(1) in 10 DEG C of temperature, under the conditions of humidity is 78RH%, 1,2 age bat moth larvaes are raised；
(2) after temperature first being adjusted to 11 DEG C, 5 days by 10 DEG C, then temperature is adjusted to 12 DEG C；In 12 DEG C of temperature, humidity
Under the conditions of 72RH%, 3 age bat moth larvaes are raised；
(3) after temperature first being adjusted to 11 DEG C, 5 days by 12 DEG C, then temperature is adjusted to 10 DEG C；After 5 days, then temperature dropped
To 9 DEG C；In 9 DEG C of temperature, under the conditions of humidity is 75RH%, 4 age bat moth larvaes are raised；
(4) after temperature first being raised to 10 DEG C, 5 days by 9 DEG C, then temperature risen to 11 DEG C by 10 DEG C；After 5 days, then by temperature
Rise to 12 DEG C；In 12 DEG C of temperature, under the conditions of humidity is 62RH%, 5 age bat moth larvaes are raised.
The above-mentioned method for breeding of the present invention is to operate to complete in gnotobasis, and the feed used in bat moth larvae is
The natural feed that the original producton location of cordyceps sinensis is cultivated, natural feed is handled before preparing with water ion sterilizing machine, in superclean bench
It is put into the culture dish that high-temperature sterilization sterilized with preparing flakiness after 2 disinfections of alcohol and dry handling.
The embodiment of the present invention 1~5 is selected to be compared with other cultural methods as experimental group, wherein, comparative example 1
Method is in the incubation of 3~5 age stage larvaes, temperature is controlled at 10~12 DEG C, and humid control is trained in 70RH%, every group
Support 100 bat moth larvae (worm sources：Qinghai Men Yuanxian cordyceps sinensis producing region), concrete outcome is shown in Table 1：
The 1 bat moth larva survival rate table of comparisons of table
Conclusion：The survival rate of the bat moth larvae of present technology is only 42% or so, passes through the method for breeding energy of the present invention
It is enough that the survival rate raising of bat moth larvae is reached 75%.
The above description is merely a specific embodiment, but protection scope of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can readily occur in change or replacement, should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
- A kind of 1. method for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that the method includes following steps Suddenly：(1) in 10~12 DEG C of temperature, under the conditions of humidity is 70~75RH%, 3 age bat moth larvaes are raised；(2) in 7~9 DEG C of temperature, under the conditions of humidity is 70~75RH%, 4 age bat moth larvaes are raised；(3) in 9~12 DEG C of temperature, under the conditions of humidity is 60~65RH%, 5 age bat moth larvaes are raised.
- 2. the method according to claim 1 for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that 1,2 During age bat moth larva raising, controlled at 9~10 DEG C, humidity is 75~80RH%.
- 3. the method according to claim 1 for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that step (1) when being converted to step (2), temperature gradually reduces to 7~9 DEG C.
- 4. the method according to claim 3 for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that step (1) when being converted to step (2), 1 DEG C is reduced within every 5 days until temperature reaches 7~9 DEG C.
- 5. the method according to claim 1 for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that step (2) when being converted to step (3), temperature is gradually increased to 9~12 DEG C.
- 6. the method according to claim 5 for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that step (2) when being converted to step (3), 1 DEG C is increased within every 5 days until temperature reaches 9~12 DEG C.
- 7. the method according to claim 1 for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that 2 ages When raising of the raising of bat moth larvae to 3 age bat moth larvaes converts, temperature is gradually increased to 10~12 DEG C.
- 8. the method according to claim 1 for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that 2 ages When raising of the raising of bat moth larvae to 3 age bat moth larvaes converts, 1 DEG C is increased within every 5 days until temperature reaches 10~12 DEG C.
- 9. the method according to claim 1 for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that in bat In the feeding process of Hepialus larva, sterile working is kept.
- 10. the method according to claim 1 for improving low altitude area raising bat moth survival rate of larvae, which is characterized in that In the feeding process of bat moth larvae, feed is replaced within every 15~25 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810216178.8A CN108184773B (en) | 2018-03-15 | 2018-03-15 | Method for improving survival rate of low-altitude bred bat moth larvae |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810216178.8A CN108184773B (en) | 2018-03-15 | 2018-03-15 | Method for improving survival rate of low-altitude bred bat moth larvae |
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| CN108184773A true CN108184773A (en) | 2018-06-22 |
| CN108184773B CN108184773B (en) | 2020-03-31 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113686842A (en) * | 2020-12-10 | 2021-11-23 | 重庆市中药研究院 | Method for rapidly detecting development process of bat moth pupae |
| CN113973784A (en) * | 2021-12-09 | 2022-01-28 | 河南农业大学 | Method for preventing insects, ant lions, from killing each other by artificial feeding and application thereof |
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|---|---|---|---|---|
| CN103081869A (en) * | 2012-08-16 | 2013-05-08 | 重庆市中药研究院 | Method for artificially cultivating cordyceps sinensis at low altitude |
| CN103168753A (en) * | 2013-04-17 | 2013-06-26 | 香格里拉县宜康宝生物科技有限公司 | Method for culturing hepialus larvae indoors |
| CN103651262B (en) * | 2012-09-20 | 2015-10-14 | 乳源南岭好山好水冬虫夏草有限公司 | The method of the survival rate of the bat moth larvae after body fluid is got in a kind of raising |
| CN106070077A (en) * | 2016-07-02 | 2016-11-09 | 广东东阳光药业有限公司 | The method of host of Cordyceps sinensis larva is raised in a kind of rotation |
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2018
- 2018-03-15 CN CN201810216178.8A patent/CN108184773B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081869A (en) * | 2012-08-16 | 2013-05-08 | 重庆市中药研究院 | Method for artificially cultivating cordyceps sinensis at low altitude |
| CN103651262B (en) * | 2012-09-20 | 2015-10-14 | 乳源南岭好山好水冬虫夏草有限公司 | The method of the survival rate of the bat moth larvae after body fluid is got in a kind of raising |
| CN103168753A (en) * | 2013-04-17 | 2013-06-26 | 香格里拉县宜康宝生物科技有限公司 | Method for culturing hepialus larvae indoors |
| CN106070077A (en) * | 2016-07-02 | 2016-11-09 | 广东东阳光药业有限公司 | The method of host of Cordyceps sinensis larva is raised in a kind of rotation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113686842A (en) * | 2020-12-10 | 2021-11-23 | 重庆市中药研究院 | Method for rapidly detecting development process of bat moth pupae |
| CN113686842B (en) * | 2020-12-10 | 2023-06-30 | 重庆市中药研究院 | Method for rapidly detecting development process of hepialus pupae |
| CN113973784A (en) * | 2021-12-09 | 2022-01-28 | 河南农业大学 | Method for preventing insects, ant lions, from killing each other by artificial feeding and application thereof |
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| CN108184773B (en) | 2020-03-31 |
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