CN102487897A - Method for raising tobacco budworms massively - Google Patents
Method for raising tobacco budworms massively Download PDFInfo
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- CN102487897A CN102487897A CN2011103867560A CN201110386756A CN102487897A CN 102487897 A CN102487897 A CN 102487897A CN 2011103867560 A CN2011103867560 A CN 2011103867560A CN 201110386756 A CN201110386756 A CN 201110386756A CN 102487897 A CN102487897 A CN 102487897A
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- tobacco budworm
- oriental tobacco
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Abstract
The invention provides a method for raising tobacco budworms massively, which comprises the sequential steps of obtaining imagoes by pupa emergence, mating the imagoes for oviposition, performing sterilization treatment to the surfaces of oviposition gauzes, hatching eggs and raising larvas, and enabling the larvas to pupate and sterilizing pupa bodies. By means of the method, incidence rate of the tobacco budworms in the massively raising process can be reduced, and continuous multiple-generation healthy breeding of test insects is ensured.
Description
Technical field
The present invention relates to bio-artificial and raise the field, be specifically related to a kind of method of extensive raising oriental tobacco budworm.
Background technology
Setting up the oriental tobacco budworm strain, optimize oriental tobacco budworm artificial feeding technology, provide a large amount of growths neat, healthy confession examination worm, is the basis of insect Physiology and biochemistry, entomological genetics and entomological genetics research.The indoor mass rearing of oriental tobacco budworm if wherein some link starts a leak, will cause the oriental tobacco budworm pathogenic infection, and the danger of extinction appears in population.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method of extensive raising oriental tobacco budworm, and this method can reduce the incidence of disease in the extensive feeding process of oriental tobacco budworm, guarantees that the continuous multi-generation health of examination worm is bred.
The technical scheme that the present invention adopted is:
A kind of method of extensive raising oriental tobacco budworm may further comprise the steps successively:
(1) pupa turns into adult;
(2) the adult pairing is laid eggs;
(3) gauze surface of laying eggs is disinfected;
(4) ovum hatching, larva raising;
(5) larvae pupation, the pupal cell sterilization.
Said step is put into wire mesh cage with the worm pupa in (1), and the wire mesh cage size is 20CM * 20CM * 30CM, and the wire mesh cage top covers a gauze; Pallet of below pad; Put into low amounts of water in the pallet, the wire mesh cage bed hedgehopping is placed on the pallet top, drops into 30 in each wire mesh cage to (each 30 of male and female) oriental tobacco budworm pupa; In wire mesh cage, place a culture dish, place cotton mass in the culture dish.From when oriental tobacco budworm is sprouted wings, place black cloth around the wire mesh cage, control photoperiod Wei Ri ︰ night=16 ︰ 8; Inject sucrose water to cotton mass every day, because the adult that comes out that sprouts wings needs in time to draw extra-nutrition sucrose water; Per two days with the replacing of the water in the pallet once, some scales that can be scattered because adult circles in the air.
In the said step (2) from the beginning of laying eggs, the gauze of wire mesh cage top, change once every day, adult can be with the ovum product on gauze.
In the said step (3) with the gauze of collecting in the step (2) of laying eggs; Soak 15min night with 15% pasteurization, water rinsing 3 to 5 times gently then, hang airing; Put into plastic sack; Be kept in 4 ℃ of refrigerators, according to experiment progress monitoring brooding time, the gauze of laying eggs is kept at can hatching in month in the refrigerator.
The larva that hatches from ovum cloth in the said step (4), every two are put into one and refer to administration-management, and each refers to put in the administration-management oriental tobacco budworm feed; With the administration-management of the stifled tightly finger of cotton plug; When raising for three ages, will be wherein one forward in the finger administration-management newly, guarantee that each refers to oriental tobacco budworm of administration-management; Raised for six age Mos always, raise 10 days left and right sides larvae pupations.
After 2 to 3 days, collect the worm pupa at larvae pupation in the said step (5), rinse well with deionized water earlier; Soak 5min with 1% pasteurization liquid then; Clean then, through the male and female characteristic of division, placed apart after the evaluation differentiation male and female; Pupa is placed on the paper handkerchief that remains humidity, the indoor temperature that pupa is placed in control at 27 ℃, humidity 75%.
Said step (1) is preceding fumigates with fumigant receptacle, airtight stifling more than 8 hours.
The raising apparatus that said each step is used all is the raising apparatus of sterilizing, and wherein glassware was with 120 ℃ of sterilizations of high temperature 30 hours, and other vessel are used the pasteurization liquid disinfectant, sterilizes with potassium permanganate in ground.
Compared with prior art; The present invention has following remarkable advantage and beneficial effect: the raising method of oriental tobacco budworm of the present invention at first guarantees suitable foodstuff and suitable growing condition; Next prevents the outburst of insect cause of disease in the environment; Feeding process adopts single head to raise, and feeding environment is raised the used vessel of step with each and carried out disinfection.During hatching just, the oriental tobacco budworm individual difference is little, but three ages during tube are chosen individual larva of uniform size, with the uniformity that guarantees that the later stage pupates and sprouts wings.The chance of insect transmission of pathogen has been reduced in this laboratory in feeding process, improved the survival rate of oriental tobacco budworm population.The present invention adopts the method for a kind of extensive raising oriental tobacco budworm that technique scheme provides; Collection, imago breeding and the mating of the sterilization through constantly perfect ovum, larva raising, the pupa improvement of each link such as lay eggs; Reduce the chance that the insect disease original is propagated, reduced the incidence of disease of insect in raising, guaranteed the healthy breeding of many generations of oriental tobacco budworm continous-stable; Simple to operate, significantly improved the efficient of raising.
Embodiment
Below in conjunction with embodiment the present invention is made further specific descriptions, but be not limited to this.
A kind of method of extensive raising oriental tobacco budworm may further comprise the steps successively:
(1) before raising, receptacle is fumigated with fumigant, airtight stifling more than 8 hours; Raising apparatus to using in the feeding process carries out disinfection, and wherein glassware was with 120 ℃ of sterilizations of high temperature 30 hours, and other vessel are used the pasteurization liquid disinfectant, sterilizes with potassium permanganate in ground.
(2) pupa turns into adult: the worm pupa is put into wire mesh cage, and the wire mesh cage size is 20CM * 20CM * 30CM, and the wire mesh cage top covers a gauze; Pallet of below pad; Put into low amounts of water in the pallet, the wire mesh cage bed hedgehopping is placed on the pallet top, drops into 30 in each wire mesh cage to (each 30 of male and female) oriental tobacco budworm pupa; In wire mesh cage, place a culture dish, place cotton mass in the culture dish.From when oriental tobacco budworm is sprouted wings, place black cloth around the wire mesh cage, control photoperiod Wei Ri ︰ night=16 ︰ 8 injects sucrose water to cotton mass every day, the water in the pallet is changed once in per two days.
(3) the adult pairing is laid eggs: the beginning of laying eggs certainly, and the gauze of wire mesh cage top, change once every day, and adult can produce ovum on gauze, and mark is changed the date.
(4) surface sterilization of ovum cloth is handled: with the gauze of collecting in the step (2) of laying eggs; Soak 15min night with 15% pasteurization, water rinsing 3 to 5 times gently then, hang airing; Put into plastic sack; Be kept in 4 ℃ of refrigerators, according to experiment progress monitoring brooding time, the gauze of laying eggs is kept at can hatching in month in the refrigerator.
(5) ovum hatching, larva raising: from the larva that ovum cloth hatches, every two is put into one and is referred to administration-management; Each refers to put in the administration-management oriental tobacco budworm feed, with the administration-management of the stifled tightly finger of cotton plug, when raising for three ages; Will be wherein one forward in the finger administration-management newly; Guarantee that each refers to oriental tobacco budworm of administration-management, raised for six age Mos always, raise 10 days left and right sides larvae pupations.
(6) larvae pupation, pupal cell sterilization: after 2 to 3 days, collect the worm pupa at larvae pupation; Rinse well earlier, soak 5min, cleaning then with 1% pasteurization liquid then with deionized water; Through the male and female characteristic of division; Identify to distinguish after the male and female placed apartly, pupa be placed on the paper handkerchief that remains humidity, the indoor temperature that pupa is placed in control at 27 ℃, humidity 75%.
The above embodiment of the present invention is can not be used to limit the present invention to explanation of the present invention, and implication suitable with claims of the present invention and any change in the scope all should be thought to be included in the scope of claims.
Claims (8)
1. extensive method of raising oriental tobacco budworm is characterized in that: may further comprise the steps successively:
(1) pupa turns into adult;
(2) the adult pairing is laid eggs;
(3) gauze surface of laying eggs is disinfected;
(4) ovum hatching, larva raising;
(5) larvae pupation, the pupal cell sterilization.
2. the method for a kind of extensive raising oriental tobacco budworm according to claim 1 is characterized in that: said step is put into wire mesh cage with the worm pupa in (1), and the wire mesh cage size is 20CM * 20CM * 30CM; The wire mesh cage top covers a gauze; Pad pallet in below is put into low amounts of water in the pallet, drop into 30 pairs of oriental tobacco budworm pupas in each wire mesh cage; In wire mesh cage, place a culture dish, place cotton mass in the culture dish; From when oriental tobacco budworm is sprouted wings, place black cloth around the wire mesh cage, control photoperiod Wei Ri ︰ night=16 ︰ 8 injects sucrose water to cotton mass every day, the water in the pallet is changed once in per two days.
3. the method for a kind of extensive raising oriental tobacco budworm according to claim 1 is characterized in that: from laying eggs beginning, the gauze of wire mesh cage top is changed once every day in the said step (2).
4. the method for a kind of extensive raising oriental tobacco budworm according to claim 1; It is characterized in that: with the gauze of collecting in the step (2) of laying eggs, soak 15min night in the said step (3), water rinsing 3 to 5 times gently then with 15% pasteurization; Hang airing; Put into plastic sack, be kept in 4 ℃ of refrigerators, according to experiment progress monitoring brooding time.
5. the method for a kind of extensive raising oriental tobacco budworm according to claim 1 is characterized in that: the larva that hatches from ovum cloth in the said step (4), every two are put into one and refer to administration-management; Each refers to put in the administration-management oriental tobacco budworm feed, with the administration-management of the stifled tightly finger of cotton plug, when raising for three ages; Will be wherein one forward in the finger administration-management newly; Each refers to oriental tobacco budworm of administration-management, raises for six age Mos always, raises 10 days left and right sides larvae pupations.
6. the method for a kind of extensive raising oriental tobacco budworm according to claim 1 is characterized in that: after 2 to 3 days, collect the worm pupa at larvae pupation in the said step (5); Rinse well with deionized water earlier; Soak 5min with 1% pasteurization liquid then, clean then, identify distinguish after the male and female placed apart; Pupa is placed on the paper handkerchief that remains humidity, the indoor temperature that pupa is placed in control at 27 ℃, humidity 75%.
7. the method for a kind of extensive raising oriental tobacco budworm according to claim 1 is characterized in that: said step (1) is preceding fumigates with fumigant receptacle, airtight stifling more than 8 hours.
8. the method for a kind of extensive raising oriental tobacco budworm according to claim 1; It is characterized in that: the raising apparatus that said each step is used all is the raising apparatus of sterilizing; Wherein glassware was with 120 ℃ of sterilizations of high temperature 30 hours; Other vessel are used the pasteurization liquid disinfectant, sterilize with potassium permanganate in ground.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103867560A CN102487897A (en) | 2011-11-29 | 2011-11-29 | Method for raising tobacco budworms massively |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103867560A CN102487897A (en) | 2011-11-29 | 2011-11-29 | Method for raising tobacco budworms massively |
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| Publication Number | Publication Date |
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| CN102487897A true CN102487897A (en) | 2012-06-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103867560A Pending CN102487897A (en) | 2011-11-29 | 2011-11-29 | Method for raising tobacco budworms massively |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355257A (en) * | 2013-07-15 | 2013-10-23 | 云南大学 | Breeding method and breeding box for insect larvae |
| CN103931566A (en) * | 2014-04-25 | 2014-07-23 | 浙江农林大学天目学院 | Method for inducing diapause of oriental tobacco budworms |
-
2011
- 2011-11-29 CN CN2011103867560A patent/CN102487897A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 张勇等: "人工饲料与天然饲料饲养条件下烟青虫的生长发育和繁殖力比较", 《昆虫知识》 * |
| 李咏军等: "烟青虫人工大量饲养技术的研究", 《核农学报》 * |
| 杨录明等: "烟青虫生物学特性观察", 《云南农业大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355257A (en) * | 2013-07-15 | 2013-10-23 | 云南大学 | Breeding method and breeding box for insect larvae |
| CN103931566A (en) * | 2014-04-25 | 2014-07-23 | 浙江农林大学天目学院 | Method for inducing diapause of oriental tobacco budworms |
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Application publication date: 20120613 |