CN107295990A - A kind of Macrobrachium rosenbergii SPF(It is virus-free)Offspring breeding method - Google Patents

A kind of Macrobrachium rosenbergii SPF(It is virus-free)Offspring breeding method Download PDF

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Publication number
CN107295990A
CN107295990A CN201710531115.7A CN201710531115A CN107295990A CN 107295990 A CN107295990 A CN 107295990A CN 201710531115 A CN201710531115 A CN 201710531115A CN 107295990 A CN107295990 A CN 107295990A
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shrimp
water
pond
close
close shrimp
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Inventor
张爱英
黄旭雄
舒琥
张勇
张宇飞
侯丽萍
王飞
陈雪峰
尤洋
高强
吴年红
戴习林
林浩然
李强
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Anhui Jiafeng Fishseed Technology Co Ltd
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Anhui Jiafeng Fishseed Technology Co Ltd
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Priority to CN201710531115.7A priority Critical patent/CN107295990A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/59Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/13Prevention or treatment of fish diseases
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention relates to a kind of Macrobrachium rosenbergii SPF(It is virus-free)Offspring breeding method.Comprise the steps:Virus-free close shrimp seed selection;The reinforced cultivating of relative shrimp overwintering phase;The raising technology of virus-free seed, the inventive method can prevent the prevalence of Opaque Muscle Disease In Macrobrachium Rosenbergii, the close shrimp nutrition of reinforcing, strengthen its immunity, it it also avoid close shrimp and produce apocleisis situation, increase quantity of exchanged water and change water frequency to keep the pure and fresh of water quality, promote close shrimp sexual gland fast-ripenin and mate and oviposit is carried out in pond.

Description

A kind of Macrobrachium rosenbergii SPF(It is virus-free)Offspring breeding method
Technical field
The present invention relates to a kind of mating system of prevention Opaque Muscle Disease In Macrobrachium Rosenbergii, more particularly to a kind of Macrobrachium rosenbergii SPF(It is virus-free)Offspring breeding method.
Background technology
Opaque Muscle Disease In Macrobrachium Rosenbergii is also known as white body disease, white smear disease, is the one kind for betiding the Macrobrachium rosenbergii seed stage There is muscle gonorrhoea, hickie or white smear symptom in disease, ill shrimp body, and mortality can occur within a couple of days, and the death rate may be up to More than 60%, the main harm as current Macrobrachium rosenbergii breeding, cultivation.Research shows, the disease of Opaque Muscle Disease In Macrobrachium Rosenbergii Originally it was M.rosenbergii Nodavirus, route of transmission is based on vertical transmission, but its horizontal transmission also has extremely strong infection ability.I Company has carried out the virus-free seed breeding technology of Macrobrachium rosenbergii with the viral diagnosis technology of ELISA method and RT-PCR method Research, successfully provides virus-free seed shrimp and virus-free seed, and effectively prevent sieve for the main nursery garden of China and culture zone The prevalence of family name's pond crayfish whitish muscle diseases.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide one kind, the present invention relates to prevention Macrobrachium rosenbergii muscle gonorrhoea The prevalence of disease, and Macrobrachium rosenbergii SPF(It is virus-free)Offspring breeding method, implements Macrobrachium rosenbergii SPF(It is virus-free)Seed breeding.
The technical scheme is that:Comprise the steps:
First, virus-free close shrimp seed selection:1)Seed selects:Every year by the end of April~5 month at the beginning of, from from numerous or there is no morbidity history nursery The high-quality health desalination seed of production, specification is in long more than the 0.82cm of body, and individual is uniform, physique healthy and strong, and with stronger inverse Trip ability, and the shrimp seedling being negative by M.rosenbergii Nodavirus TAS-ELISA method detection;
2)Shrimp seedling is temporarily supported:Tu Chi is cultivated in cement pit or greenhouse of the selection with quarantine measures and Jing Guo strict sterilization, Breeding density in cement pit is that the breeding density in native pond is 2500~2950 tails/m2 in 4500~4950 tails/m2, greenhouse, 25 DEG C of water temperature, feed uses egg custard, prawn slice, Macrobrachium rosenbergii mixed fodder with better nutritivity value etc.;During temporarily supporting, daily Shrimp seedling active situation, situation of ingesting etc. are examined, suspicious shrimp seedling is selected in time and carries out Viral diagnosis, in 1 month temporarily support Viral diagnosis and testing result in phase at least through 8 times are negative;After May 20, outer pool water temperature is more than 22 DEG C, by shrimp Seedling is put into the pond culture with quarantine measures, and the pond should be without morbidity history, and requires before shrimp seedling is put in a suitable place to breed by strictly disappearing Poison;
3)Pond culture:Carried out between June~October in pond culture, parent culture area surrounding 1000m without Macrobrachium rosenbergii culturing pool, Cultivate area periphery and isolation is blocked with protection network, be both parent culture area, be Conservation area again;Single 20 mu of left sides of culture pond specification The right side, more than 2.5 meters of the depth of water, water inlet enters pond with close eye screen cloth bag filtration to prevent external shrimp;Discharge outlet net cloth cover, to prevent pond Shrimp is fled;
4)Disinfection and isolation measure during shrimp seed rearing:Cultivation during shrimp seedling temporarily foster, pond culture is with water with cistern pair Water source is precipitated and disinfected, while noting being used as breeding feed without using of unknown origin frost wild fishes;
5)Close shrimp selects:Early October carries out close shrimp and selected;Select that physically fit, appendage is complete, shrimp body of the whole body without focus, and it is female Male ratio is 2~3:l;
2nd, the reinforced cultivating of relative shrimp overwintering phase:
1)The pretreatment of wintering pond:Wintering pond is soaked more than l weeks using preceding with 30ppm liquor potassic permanganates, is scrubbed after cleaning up the pond Totally, it is stand-by;
2)The regulation and control of control environment condition:During surviving the winter, water temperature is controlled at 2l DEG C -22 DEG C;At the initial stage of surviving the winter, because shrimp body has just entered Enter greenhouse and mechanical damage is heavier, water temperature can be properly increased to l DEG C~2 DEG C, after shrimp body food ration increases and recovers constitution Water temperature of surviving the winter is reduced to again;
3)Feedstuff feeding:Feed is the basis of close shrimp gonad development, and feedstuff feeding should ensure the nutritional need of close shrimp, protect again Its palatability is demonstrate,proved, during surviving the winter, can use based on artifical compound feed, be supplemented being fed with animal bait, and it is fixed Phase adds the close shrimp nutrition of the reinforcing such as B B-complex, immune polysaccharide in feed, strengthens its immunity, it also avoid close shrimp and produces Apocleisis situation;
4)Strengthen management:The water source of breeding water will be ensured not by pathogen contamination, and periodically water body of surviving the winter is carried out disinfection, daily Strict sterilization should also be carried out to the instrument in greenhouse and workshop;
5)Viral diagnosis periodically is carried out to close shrimp:The daily close shrimp of observation ingests situation, active situation etc., such as finds suspicious close shrimp Viral diagnosis is carried out immediately, during surviving the winter, monthly at least ensure there is the Viral diagnosis record of more than 1 time;
3rd, the raising technology of virus-free seed:
1)The pretreatment at water source:Pond water filling when water temperature is relatively low in the winter time, and water is carried out disinfection with quick lime, by 1 Month~precipitation of 2 months, disinfect, the water quality index of water can reach preferable state in nursery season;
2)The reinforced cultivating of close shrimp:In whole nursery stage, feeding for animal bait should be increased, and add compound in feed The materials such as vitamin, immune polysaccharide, day feeding volume is the 5%~7% of shrimp body body weight, and adjusted at any time according to shrimp body situation of ingesting Feeding volume, increases quantity of exchanged water and changes water frequency to keep the pure and fresh of water quality, promotes close shrimp sexual gland fast-ripenin and is carried out in pond Mate and oviposit;
3)Disease control:Using putting prevention first, anti-to overweight the comprehensive preventive health measures controlled, nursery pond is with seedling-cultivating tool using front and rear Strict washing and sterilizing is wanted, after each shrimping operation, with the close shrimp ponds of the potassium permanganate thorough disinfection of high concentration, water for larval nursing is in water inlet The yarn thin,tough silk of the mesh of Shi Keyong 200 is filtered to water source, periodically carries out Viral diagnosis to the young (the young shrimp after particularly desalinating).
The inventive method can prevent the prevalence of Opaque Muscle Disease In Macrobrachium Rosenbergii, and the close shrimp nutrition of reinforcing strengthens its immunity, Avoid close shrimp and produce apocleisis situation, increase quantity of exchanged water and change water frequency to keep the pure and fresh of water quality, promote close shrimp sexual gland quick It is ripe and mate and oviposit is carried out in pond.
Embodiment
A kind of Macrobrachium rosenbergii SPF(It is virus-free)Offspring breeding method, comprises the steps:
First, virus-free close shrimp seed selection:1)Seed selects:Every year by the end of April~5 month at the beginning of, from from numerous or there is no morbidity history nursery The high-quality health desalination seed of production, specification is in long more than the 0.82cm of body, and individual is uniform, physique healthy and strong, and with stronger inverse Trip ability, and the shrimp seedling being negative by M.rosenbergii Nodavirus TAS-ELISA method detection;
2)Shrimp seedling is temporarily supported:Tu Chi is cultivated in cement pit or greenhouse of the selection with quarantine measures and Jing Guo strict sterilization, Breeding density in cement pit is that the breeding density in native pond is 2500~2950 tails/m2 in 4500~4950 tails/m2, greenhouse, 25 DEG C of water temperature, feed uses egg custard, prawn slice, Macrobrachium rosenbergii mixed fodder with better nutritivity value etc.;During temporarily supporting, daily Shrimp seedling active situation, situation of ingesting etc. are examined, suspicious shrimp seedling is selected in time and carries out Viral diagnosis, in 1 month temporarily support Viral diagnosis and testing result in phase at least through 8 times are negative;After May 20, outer pool water temperature is more than 22 DEG C, by shrimp Seedling is put into the pond culture with quarantine measures, and the pond should be without morbidity history, and requires before shrimp seedling is put in a suitable place to breed by strictly disappearing Poison;
3)Pond culture:Carried out between June~October in pond culture, parent culture area surrounding 1000m without Macrobrachium rosenbergii culturing pool, Cultivate area periphery and isolation is blocked with protection network, be both parent culture area, be Conservation area again;Single 20 mu of left sides of culture pond specification The right side, more than 2.5 meters of the depth of water, water inlet enters pond with close eye screen cloth bag filtration to prevent external shrimp;Discharge outlet net cloth cover, to prevent pond Shrimp is fled;
4)Disinfection and isolation measure during shrimp seed rearing:Cultivation during shrimp seedling temporarily foster, pond culture is with water with cistern pair Water source is precipitated and disinfected, while noting being used as breeding feed without using of unknown origin frost wild fishes;
5)Close shrimp selects:Early October carries out close shrimp and selected;Select that physically fit, appendage is complete, shrimp body of the whole body without focus, and it is female Male ratio is 2~3:l;
2nd, the reinforced cultivating of relative shrimp overwintering phase:
1)The pretreatment of wintering pond:Wintering pond is soaked more than l weeks using preceding with 30ppm liquor potassic permanganates, is scrubbed after cleaning up the pond Totally, it is stand-by;
2)The regulation and control of control environment condition:During surviving the winter, water temperature is controlled at 2l DEG C -22 DEG C;At the initial stage of surviving the winter, because shrimp body has just entered Enter greenhouse and mechanical damage is heavier, water temperature can be properly increased to l DEG C~2 DEG C, after shrimp body food ration increases and recovers constitution Water temperature of surviving the winter is reduced to again;
3)Feedstuff feeding:Feed is the basis of close shrimp gonad development, and feedstuff feeding should ensure the nutritional need of close shrimp, protect again Its palatability is demonstrate,proved, during surviving the winter, can use based on artifical compound feed, be supplemented being fed with animal bait, and it is fixed Phase adds the close shrimp nutrition of the reinforcing such as B B-complex, immune polysaccharide in feed, strengthens its immunity, it also avoid close shrimp and produces Apocleisis situation;
4)Strengthen management:The water source of breeding water will be ensured not by pathogen contamination, and periodically water body of surviving the winter is carried out disinfection, daily Strict sterilization should also be carried out to the instrument in greenhouse and workshop;
5)Viral diagnosis periodically is carried out to close shrimp:The daily close shrimp of observation ingests situation, active situation etc., such as finds suspicious close shrimp Viral diagnosis is carried out immediately, during surviving the winter, monthly at least ensure there is the Viral diagnosis record of more than 1 time;
3rd, the raising technology of virus-free seed:
1)The pretreatment at water source:Pond water filling when water temperature is relatively low in the winter time, and water is carried out disinfection with quick lime, by 1 Month~precipitation of 2 months, disinfect, the water quality index of water can reach preferable state in nursery season;
2)The reinforced cultivating of close shrimp:In whole nursery stage, feeding for animal bait should be increased, and add compound in feed The materials such as vitamin, immune polysaccharide, day feeding volume is the 5%~7% of shrimp body body weight, and adjusted at any time according to shrimp body situation of ingesting Feeding volume, increases quantity of exchanged water and changes water frequency to keep the pure and fresh of water quality, promotes close shrimp sexual gland fast-ripenin and is carried out in pond Mate and oviposit;
3)Disease control:Using putting prevention first, anti-to overweight the comprehensive preventive health measures controlled, nursery pond is with seedling-cultivating tool using front and rear Strict washing and sterilizing is wanted, after each shrimping operation, with the close shrimp ponds of the potassium permanganate thorough disinfection of high concentration, water for larval nursing is in water inlet The yarn thin,tough silk of the mesh of Shi Keyong 200 is filtered to water source, periodically carries out Viral diagnosis to the young (the young shrimp after particularly desalinating).

Claims (1)

1. a kind of Macrobrachium rosenbergii SPF(It is virus-free)Offspring breeding method, it is characterised in that:Comprise the steps:
First, virus-free close shrimp seed selection:1)Seed selects:Every year by the end of April~5 month at the beginning of, from from numerous or there is no morbidity history nursery The high-quality health desalination seed of production, specification is in long more than the 0.82cm of body, and individual is uniform, physique healthy and strong, and with stronger inverse Trip ability, and the shrimp seedling being negative by M.rosenbergii Nodavirus TAS-ELISA method detection;
2)Shrimp seedling is temporarily supported:Tu Chi is cultivated in cement pit or greenhouse of the selection with quarantine measures and Jing Guo strict sterilization, Breeding density in cement pit is that the breeding density in native pond is 2500~2950 tails/m2 in 4500~4950 tails/m2, greenhouse, 25 DEG C of water temperature, feed uses egg custard, prawn slice, Macrobrachium rosenbergii mixed fodder with better nutritivity value etc.;During temporarily supporting, daily Shrimp seedling active situation, situation of ingesting etc. are examined, suspicious shrimp seedling is selected in time and carries out Viral diagnosis, in 1 month temporarily support Viral diagnosis and testing result in phase at least through 8 times are negative;After May 20, outer pool water temperature is more than 22 DEG C, by shrimp Seedling is put into the pond culture with quarantine measures, and the pond should be without morbidity history, and requires before shrimp seedling is put in a suitable place to breed by strictly disappearing Poison;
3)Pond culture:Carried out between June~October in pond culture, parent culture area surrounding 1000m without Macrobrachium rosenbergii culturing pool, Cultivate area periphery and isolation is blocked with protection network, be both parent culture area, be Conservation area again;Single 20 mu of left sides of culture pond specification The right side, more than 2.5 meters of the depth of water, water inlet enters pond with close eye screen cloth bag filtration to prevent external shrimp;Discharge outlet net cloth cover, to prevent pond Shrimp is fled;
4)Disinfection and isolation measure during shrimp seed rearing:Cultivation during shrimp seedling temporarily foster, pond culture is with water with cistern pair Water source is precipitated and disinfected, while noting being used as breeding feed without using of unknown origin frost wild fishes;
5)Close shrimp selects:Early October carries out close shrimp and selected;Select that physically fit, appendage is complete, shrimp body of the whole body without focus, and it is female Male ratio is 2~3:l;
2nd, the reinforced cultivating of relative shrimp overwintering phase:
1)The pretreatment of wintering pond:Wintering pond is soaked more than l weeks using preceding with 30ppm liquor potassic permanganates, is scrubbed after cleaning up the pond Totally, it is stand-by;
2)The regulation and control of control environment condition:During surviving the winter, water temperature is controlled at 2l DEG C -22 DEG C;At the initial stage of surviving the winter, because shrimp body has just entered Enter greenhouse and mechanical damage is heavier, water temperature can be properly increased to l DEG C~2 DEG C, after shrimp body food ration increases and recovers constitution Water temperature of surviving the winter is reduced to again;
3)Feedstuff feeding:Feed is the basis of close shrimp gonad development, and feedstuff feeding should ensure the nutritional need of close shrimp, protect again Its palatability is demonstrate,proved, during surviving the winter, can use based on artifical compound feed, be supplemented being fed with animal bait, and it is fixed Phase adds the close shrimp nutrition of the reinforcing such as B B-complex, immune polysaccharide in feed, strengthens its immunity, it also avoid close shrimp and produces Apocleisis situation;
4)Strengthen management:The water source of breeding water will be ensured not by pathogen contamination, and periodically water body of surviving the winter is carried out disinfection, daily Strict sterilization should also be carried out to the instrument in greenhouse and workshop;
5)Viral diagnosis periodically is carried out to close shrimp:The daily close shrimp of observation ingests situation, active situation etc., such as finds suspicious close shrimp Viral diagnosis is carried out immediately, during surviving the winter, monthly at least ensure there is the Viral diagnosis record of more than 1 time;
3rd, the raising technology of virus-free seed:
1)The pretreatment at water source:Pond water filling when water temperature is relatively low in the winter time, and water is carried out disinfection with quick lime, by 1 Month~precipitation of 2 months, disinfect, the water quality index of water can reach preferable state in nursery season;
2)The reinforced cultivating of close shrimp:In whole nursery stage, feeding for animal bait should be increased, and add compound in feed The materials such as vitamin, immune polysaccharide, day feeding volume is the 5%~7% of shrimp body body weight, and adjusted at any time according to shrimp body situation of ingesting Feeding volume, increases quantity of exchanged water and changes water frequency to keep the pure and fresh of water quality, promotes close shrimp sexual gland fast-ripenin and is carried out in pond Mate and oviposit;
3)Disease control:Using putting prevention first, anti-to overweight the comprehensive preventive health measures controlled, nursery pond is with seedling-cultivating tool using front and rear Strict washing and sterilizing is wanted, after each shrimping operation, with the close shrimp ponds of the potassium permanganate thorough disinfection of high concentration, water for larval nursing is in water inlet The yarn thin,tough silk of the mesh of Shi Keyong 200 is filtered to water source, periodically carries out Viral diagnosis to the young (the young shrimp after particularly desalinating).
CN201710531115.7A 2017-07-03 2017-07-03 A kind of Macrobrachium rosenbergii SPF(It is virus-free)Offspring breeding method Pending CN107295990A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112293322A (en) * 2020-11-26 2021-02-02 武汉新河湾农业科技有限公司 Improved variety breeding method for shrimp larvae
CN113349126A (en) * 2021-07-12 2021-09-07 安徽雨泉农业科技有限公司 Macrobrachium rosenbergii paddy field culture and wild trash fish physical removal method
CN113841638A (en) * 2021-08-24 2021-12-28 中国水产科学研究院珠江水产研究所 Virus-free fry breeding method for micropterus salmoides
CN113841637A (en) * 2021-08-24 2021-12-28 中国水产科学研究院珠江水产研究所 Hybrid snakehead rhabdovirus-free fry breeding method
CN116349629A (en) * 2023-04-25 2023-06-30 浙江省淡水水产研究所 Indoor intensified cultivation method for macrobrachium rosenbergii

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112293322A (en) * 2020-11-26 2021-02-02 武汉新河湾农业科技有限公司 Improved variety breeding method for shrimp larvae
CN113349126A (en) * 2021-07-12 2021-09-07 安徽雨泉农业科技有限公司 Macrobrachium rosenbergii paddy field culture and wild trash fish physical removal method
CN113841638A (en) * 2021-08-24 2021-12-28 中国水产科学研究院珠江水产研究所 Virus-free fry breeding method for micropterus salmoides
CN113841637A (en) * 2021-08-24 2021-12-28 中国水产科学研究院珠江水产研究所 Hybrid snakehead rhabdovirus-free fry breeding method
CN116349629A (en) * 2023-04-25 2023-06-30 浙江省淡水水产研究所 Indoor intensified cultivation method for macrobrachium rosenbergii

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