CN103141670A - Myllocerinus aurolineatus adult artificial feed, preparation method of the myllocerinus aurolineatus adult artificial feed, and myllocerinus aurolineatus adult breeding method - Google Patents
Myllocerinus aurolineatus adult artificial feed, preparation method of the myllocerinus aurolineatus adult artificial feed, and myllocerinus aurolineatus adult breeding method Download PDFInfo
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Abstract
The invention relates to myllocerinus aurolineatus adult artificial feed, a preparation method of the myllocerinus aurolineatus adult artificial feed, and a myllocerinus aurolineatus adult breeding method. The myllocerinus aurolineatus adult artificial feed comprises, by weight, 15 to 20% of dry tea powder, 5 to 10% of yeast powder, 1 to 3% of honey, 0.5 to 1% of a vitamin B complex, 1 to 5% of cane sugar, 5 to 10% of soybean powder, 0.1 to 0.5% of sorbic acid, 0.1 to 0.3% of nipagin, 1 to 2% of agar, 20 to 30% of soil extract and the balance distilled water. The myllocerinus aurolineatus adult artificial feed can be preserved for a long time at a low temperature, prevents season limit on fresh tea tree leaf supply, realizes myllocerinus aurolineatus indoor breeding, and fills in the blank of the myllocerinus aurolineatus adult artificial feed study. The myllocerinus aurolineatus adult artificial feed has outstanding effects of improving an average egg laying amount of each female adult and egg hatchability. The myllocerinus aurolineatus adult artificial feed adopts the easily acquired raw materials and has a low cost. The myllocerinus aurolineatus adult breeding method has simple processes. Through the myllocerinus aurolineatus adult breeding method, under the breeding environment, adult duration and hatchability are the same as the effects of fresh leaf breeding. The myllocerinus aurolineatus adult breeding method provides enough experimental population for deep research on fields of myllocerinus aurolineatus biology and myllocerinus aurolineatus prevention and control technology.
Description
Technical field
The present invention relates to the method for breeding of the preparation method of a kind of insect artificial diet and feed and the artificial procreation technical field of insect, particularly man-made feeds of the beautiful line weevil of a kind of tea adult and preparation method thereof and the beautiful line weevil of tea adult.
Background technology
The beautiful line weevil of tea has another name called the tealeaves weevil, belong to coleoptera, Culculionidae, beautiful line and resemble genus, be the important bud-leaf insect of tealeaves, be widely distributed on the south the Yangtze river basin tea district, occur more serious with province tea places such as Zhejiang, Jiangsu, Anhui, Fujian, Hunan, Hubei, Guangdong, Yunnan.
In 1 generation, occured in China 1 year in the beautiful line weevil of tea, survives the winter in tea clump rhizosphere table soil with larva, and begin to pupate mid-April next year, in Chongqing region, the May～June adult begin to sprout wings and the scholar, mid or late August sees eventually.Adult tea tree phase of causing harm reaches 3 wheat harvesting periods, the Sheng that the causes harm phase in June to July.The beautiful line weevil of tea is one of main To Leaf-feeding Insects in tea district, occurs serious throughout the year.Adult chews food tea tree tender leaf, and ferocious sticking up when occuring caused the bald arteries and veins of the residual leaf of tea tree, has both made the tea yield loss, damages again tree vigo(u)r.The made liquor color of killed tealeaves is muddy dark, and is broken at the bottom of leaf.Its host also has citrus, pears, peach, Chinese chestnut, oil tea and apple etc. except tea tree.Because the beautiful line weevil of tea larva, pupa are lived in soil, and adult is elytrum, prevents and treats it to the tea place and brings certain difficulty.At present, the field lacks effectively for the reasonable prophylactico-therapeutic measures of this insect, and sufficient stable worm source can guarantee it is carried out the system research in the fields such as Prevention Technique.
At present, domestic research to the insect artificial diet mainly concentrates on two fields: one is traditional resource insect field, such as spun silk insect (as silkworm), edible and feed insect (as locust, yellow meal worm), natural enemy insect (as coccinella septempunctata), view and admire insect (as butterfly), pharmaceutical insects (as eye spot blister beetle) etc.; On the other hand, need regular to provide a large amount of growths consistent worm source in order to study the insect biological nature, such as tea geometrid, tung tree looper etc.Now existing many scholars are studied man-made feeds formula, preparation and the artificial feeding of other insect of coleoptera.
Although the man-made feeds of coleoptera class insect have had some researchs, there is not yet report for the beautiful line weevil of tea adult man-made feeds and indoor feeding technical research.Due to insect Species diversity and kind difference, the research of man-made feeds had special specific aim and complexity.
Therefore, for keeping and continue population quantity and the amount reproduction of the beautiful line weevil of tea, suitable man-made feeds preparation method and method for breeding thereof are the keys that solves the breeding of the beautiful line weevil of tea adult mass rearing.
Summary of the invention
The object of the present invention is to provide a kind of man-made feeds of feeding the beautiful line weevil of tea adult.
Another object of the present invention is to provides a kind of preparation method for the beautiful line weevil of tea adult man-made feeds.
A further object of the present invention is to adopt these man-made feeds to raise the beautiful line weevil of tea adult, make the beautiful line weevil of tea adult break away from fresh leaves of tea plant and realize purpose at indoor feeding, for the system research of going deep into of completing the fields such as biology, ecology and Prevention Technique of the beautiful line weevil of tea provides sufficient experimental population.
The objective of the invention is to realize by such technical scheme:
The man-made feeds of the beautiful line weevil of a kind of tea adult, composed of the following components by weight percentage:
Dried tea powder 15%～20%, dusty yeast 5%～10%, honey 1%～3%, vitamin B compound 0.5%～1%, sucrose 1%～5%, analysis for soybean powder 5%～10%, sorbic acid 0.1～0.5%, nipalgin 0.1～0.3%, agar 1%～2%, soil extraction 20%～30%, distilled water: surplus.
The man-made feeds of the preferred beautiful line weevil of tea adult, composed of the following components by weight percentage: dried tea powder 16%, dusty yeast 6.6%, honey 1.1%, vitamin B compound 0.8%, sucrose 1.4%, analysis for soybean powder 5.4%, sorbic acid 0.1%, nipalgin 0.2%, agar 1.9%, soil extraction 25%, distilled water: surplus.
Another object of the present invention is achieved in that the preparation method of above-mentioned man-made feeds prepares as follows:
A. the preparation of raw material:
The preparation of dried tea powder: select the ripe tea leaf of giving birth to then through 60 ℃ ± 2 ℃ oven dry, pulverize, cross 60 mesh sieves, make dried tea powder; Prepare unnecessary dried tea powder and be placed in-20 ℃ of condition lower seals preservations;
The preparation of soil extraction: take the air-dry soil that sieved through 1mm soil, this soil organic matter content is not less than 30g/kg, soil thickness 0～20cm, distilled water immersion 2h, swing gently, then be that 0.1g/ml adds the water preparation by concentration, speed with 60 times/min stirs, then standing, clarification, and supernatant is soil extraction.
The preparation of agar mixed liquor: after agar powder was heated to agar and dissolves fully with distilled water, stopped heating treated that solution is cooled to 65 ℃ ± 2 ℃, adds sorbic acid, nipalgin, conventional stirring and dissolving wherein;
B. dried tea powder, analysis for soybean powder and dusty yeast are mixed;
C. sucrose, vitamin B compound and honey are mixed;
D. the mixture with step B and step C configuration mixes;
E. add in step D together with soil extraction after the agar mixed liquor being cooled to 60 ℃ ± 2 ℃, mix and obtain the beautiful line weevil of tea adult man-made feeds.
Adopt the beautiful line weevil of the tea adult man-made feeds of technique scheme preparation, can satisfy the requirement of the beautiful line weevil of tea adult foods, can preserve more than 1 month under 4 ℃ of conditions.
A further object of the present invention is achieved in that the method for the beautiful line weevil of the man-made feeds raising tea adult that the above-mentioned preparation method of employing obtains, and its concrete steps are as follows:
(1) gather the beautiful line weevil of tea pupa;
(2) pupa is placed in the vial that fills the thick sandy soil of 4cm～5cm waits to sprout wings, bottleneck seals with gauze, and temperature remains on 26 ℃ ± 1 ℃, relative humidity 80% ± 5%;
(3) adult that sprouts wings is put into the new vial that fills the thick sandy soil of 4cm～5cm, every bottle of raisings is female, each of male imago, and regularly feeding, usually carried big or small one of the man-made feeds of broad bean with cover glass in every 3 days and put into bottle; Bottleneck seals with gauze, and in bottle, temperature remains on 26 ℃ ± 1 ℃, relative humidity 80% ± 5%;
(4) for keeping a humidity in bottle, the next day, insert in bottle after soaking with a cotton pellet.
Owing to having adopted technique scheme, the present invention has advantages of as follows:
Beneficial effect of the present invention:
(1) after the beautiful line weevil of tea imago feeding man-made feeds of the present invention, broken away from the defective that fresh leaves of tea plant is subject to seasonal restrictions, thereby realized the purpose at indoor feeding, the research of having filled up the beautiful line weevil of tea man-made feeds is blank; Adopt the man-made feeds of the beautiful line weevil of tea adult to raise the beautiful line weevil of tea adult, every female egg laying amount, incubation rate are significantly improved; Adopt preferred man-made feeds to raise the beautiful line weevil of tea adult, adult is gone through 35.7 days phases, and 87.3 of egg laying amounts, incubation rate are 91.2%, and it is raised usefulness and has reached the effect that takes food the fresh leaves of tea plant feeding.
(2) raw material of the present invention easily obtain, and cost is low, and the preparation method is simple, and can satisfy the needs of the growth of the beautiful line weevil of tea adult, growth and mate and oviposit, have solved the problem that the beautiful line weevil of tea adult needs fresh leaves of tea plant to feed.
(3) rearing method of the present invention is simple, and the effect that adult is gone through the phase under this method for breeding and environment, incubation rate has reached the bright leaf of feeding is for the further investigation in the fields such as the biology of completing the beautiful line weevil of tea, Prevention Technique provides sufficient experimental population.
The specific embodiment
Below each embodiment only as explanation of the present invention, percentage wherein all by weight percentage, also all can change weight g, Kg or other unit of weight into without specified otherwise.
Table 1 is several embodiment components and the component weight percent content of the beautiful line weevil of tea adult man-made feeds:
Table 1
| Component | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| Dried tea powder | 15.0 | 16.0 | 16.0 | 15.0 |
| Dusty yeast | 5.4 | 6.6 | 5.4 | 7.4 |
| Honey | 2.2 | 1.1 | 1.8 | 2.6 |
| Vitamin B compound | 0.4 | 0.8 | 0.6 | 0.2 |
| Sucrose | 3.6 | 1.4 | 4.6 | 2.4 |
| Analysis for soybean powder | 8.2 | 5.4 | 6.6 | 7.2 |
| Sorbic acid | 0.1 | 0.1 | 0.3 | 0.4 |
| Nipalgin | 0.3 | 0.2 | 0.1 | 0.1 |
| Agar | 1.5 | 1.9 | 2.0 | 1.6 |
| Soil extraction | 22.0 | 25.0 | 20.0 | 28.0 |
| Water | Surplus | Surplus | Surplus | Surplus |
Embodiment 1:
The beautiful line weevil of a kind of tea adult man-made feeds are comprised of each component of following percentage by weight: dried tea powder 15%, dusty yeast 5.4%, honey 2.2%, vitamin B compound 0.4%, sucrose 3.6%, analysis for soybean powder 8.2%, sorbic acid 0.1%, nipalgin 0.3%, agar 1.5%, soil extraction 22%, distilled water: surplus.
The preparation method who prepares the beautiful line weevil of above-mentioned tea adult man-made feeds:
The preparation of raw material:
The preparation of dried tea powder: select the ripe tea leaf of giving birth to then through 60 ℃ ± 2 ℃ oven dry, pulverize, cross 60 mesh sieves, make dried tea powder;
The preparation of soil extraction: take the air-dry soil that sieved through 1mm soil, this soil organic matter content is not less than 30g/kg, soil thickness 20cm, distilled water immersion 2h, swing gently, then be that 0.1g/ml adds the water preparation by concentration, speed with 60 times/min stirs, then standing, clarification, and supernatant is soil extraction.
The man-made feeds of the beautiful line weevil of above-mentioned tea adult take following component by weight percentage:
A. the dried tea powder of 30g, 16.4g analysis for soybean powder and the 10.8g dusty yeast that takes mixed;
B. with 7.2g sucrose, 0.8g vitamin B compound and 4.4g honey, stir with the distilled water 20g of 40 ℃ of bacterium of going out;
C. the mixture with steps A and step B configuration mixes;
D. with the 3g agar powder with 62.8g distilled water mixing after, after being heated to agar and dissolving fully, stopped heating treats that solution is cooled to 65 ℃ ± 2 ℃, adds 0.2g sorbic acid, 0.6g nipalgin, stirring and dissolving wherein;
E. add in step C together with soil extraction 44g after the mixed liquor of step D being prepared is cooled to 60 ℃ ± 2 ℃, stir and obtain the beautiful line weevil of tea adult man-made feeds.
Adopt the method for breeding of the beautiful line weevil of above-mentioned tea adult man-made feeds, its concrete steps are as follows:
The field gathers 300 of the beautiful line weevil of tea pupas;
Put 10 pupas and wait to sprout wings in one fills the glass preserving jar of the thick sandy soil of 4cm～5cm, fill altogether 30 bottles, bottleneck seals with white cotton gauze, and temperature remains on 26 ℃, relative humidity 80%;
Select the adult male and female pairing of above-mentioned new emergence, put into a new glass preserving jar that fills the thick sandy soil of 4cm～5cm, feed 30 bottles, bottleneck seals with white cotton gauze, and temperature remains on 26 ℃, relative humidity 80%;
Carried one of man-made feeds of broad bean size with cover glass in every 3 days and put into bottle, to keep the fresh of feed; Be to keep a humidity in bottle, the next day, insert in bottle after soaking with a cotton pellet.
Embodiment 2:
The beautiful line weevil of a kind of tea adult man-made feeds, each component by following percentage by weight forms: dried tea powder 16%, dusty yeast 6.6%, honey 1.1%, vitamin B compound 0.8%, sucrose 1.4%, analysis for soybean powder 5.4%, sorbic acid 0.1%, nipalgin 0.2%, agar 1.9%, soil extraction 25%, distilled water: surplus.
The preparation method who prepares the beautiful line weevil of above-mentioned tea adult man-made feeds:
The preparation of raw material: see the preparation of the raw material in the preparation method of man-made feeds of the beautiful line weevil of the embodiment 1 above-mentioned tea of preparation adult.
Prepare above-mentioned man-made feeds and take by weight percentage following component:
A. the dried tea powder of 32g, 10.8g analysis for soybean powder and the 13.2g dusty yeast that takes mixed;
B. with 2.8g sucrose, 1.6g vitamin B compound and 2.2g honey, stir with the distilled water 20g of 40 ℃ of bacterium of going out;
C. the mixture with steps A and B configuration mixes;
D. with the 3.8g agar powder with 63g distilled water mixing after, after being heated to agar and dissolving fully, stopped heating treats that solution is cooled to 65 ℃ ± 2 ℃, adds 0.2g sorbic acid, 0.4g nipalgin, stirring and dissolving wherein;
E. add in step C together with soil extraction 50g after the mixed liquor of step D being prepared is cooled to 60 ℃ ± 2 ℃, stir and obtain the beautiful line weevil of tea adult man-made feeds.
Adopt these man-made feeds to raise the method for breeding of the beautiful line weevil of tea adult with embodiment 1.
Embodiment 3:
Prepare above-mentioned feed and take by weight percentage following component: dried tea powder 16%, dusty yeast 5.4 %, honey 1.8%, vitamin B compound 0.6%, sucrose 4.6, analysis for soybean powder 6.6%, sorbic acid 0.3%, nipalgin 0.1%, agar 2%, soil extraction 20%, distilled water are surplus.
The preparation method who prepares the beautiful line weevil of above-mentioned tea adult man-made feeds:
The preparation of raw material: see the preparation of the raw material in the preparation method of the beautiful line weevil of the embodiment 1 above-mentioned tea of preparation adult man-made feeds.
Prepare above-mentioned feed and take by weight percentage following component:
A. the dried tea powder of 32g, 13.2g analysis for soybean powder and the 10.8g dusty yeast that takes mixed;
B. with 9.2g sucrose, 1.2g vitamin B compound and 3.6g honey, stir with the distilled water 25g of 40 ℃ of bacterium of going out;
C. the mixture with steps A and B configuration mixes;
D. with the 4g agar powder with 60.4g distilled water mixing after, after being heated to agar and dissolving fully, stopped heating treats that solution is cooled to 65 ℃ ± 2 ℃, adds wherein 0.6g sorbic acid, 0.2g nipalgin stirring and dissolving;
E. add in step C together with soil extraction 40g after the mixed liquor of step D being prepared is cooled to 60 ℃ ± 2 ℃, stir and obtain the beautiful line weevil of tea adult man-made feeds.
Adopt above-mentioned man-made feeds to raise the method for breeding of the beautiful line weevil of tea adult with embodiment 1.
Embodiment 4:
Feed is comprised of each component of following percentage by weight: dried tea powder 15%, dusty yeast 7.4%, honey 2.6%, vitamin B compound 0.2%, sucrose 2.4%, analysis for soybean powder 7.2%, sorbic acid 0.4%, nipalgin 0.1%, agar 1.6%, soil extraction 28%, distilled water are surplus.
The preparation method who prepares the man-made feeds of the beautiful line weevil of above-mentioned tea adult:
The preparation of raw material: see the preparation of the raw material in the preparation method of the beautiful line weevil of the embodiment 1 above-mentioned tea of preparation adult man-made feeds.
Prepare above-mentioned feed and take by weight percentage following component:
A. the dried tea powder of 30g, 14.4g analysis for soybean powder and the 14.8g dusty yeast that takes mixed;
B. with 4.8g sucrose, 0.4g vitamin B compound and 5.2g honey, stir with the distilled water 20g of 40 ℃ of bacterium of going out;
C. the mixture with steps A and B configuration mixes;
D. with the 3.2g agar powder with 50.2g distilled water mixing after, after being heated to agar and dissolving fully, stopped heating treats that solution is cooled to 65 ℃ ± 2 ℃, adds 0.8g sorbic acid, 0.2g nipalgin, stirring and dissolving wherein;
E. add in step C together with soil extraction 56g after the mixed liquor of step D being prepared is cooled to 60 ℃ ± 2 ℃, stir and obtain the beautiful line weevil of tea adult man-made feeds.
Table 2 be according to table 1 embodiment raise the beautiful line weevil of tea adult to feed and the natural feed (bright leaf) of weight percentages of components preparation adult go through the phase, the comparable situation of every female egg laying amount and incubation rate.
Table 2
| Component | Adult go through the phase (my god) | Every female egg laying amount (grain) | Incubation rate (%) |
| Embodiment 1 | 34.1 | 81.3 | 85.6 |
| Embodiment 2 | 35.7 | 87.3 | 91.2 |
| Embodiment 3 | 33.5 | 83.2 | 82.8 |
| Embodiment 4 | 33.6 | 85.5 | 90.3 |
| Bright leaf | 37.2 | 90.6 | 93.6 |
Data analysis by table 1 and table 2, can find out: raise the beautiful line weevil of tea adult according to the man-made feeds of table 1 component and weight percentages of components preparation and all can grow and lay eggs: it has reached the effect of the bright leaf of feeding at aspects such as Life of Adult, egg laying amount, incubation rates, wherein especially take embodiment 2 as best.
The man-made feeds of the beautiful line weevil of tea of the present invention adult are simple and easy to system, and cost is also not high, and wherein dried tea powder can producing the preservation of preparation in season oneself low temperature, also can be bought on market; But the required tea garden soil ready access upon use of soil extraction is unrestricted.
Claims (8)
1. the beautiful line weevil of tea adult man-made feeds, it is characterized in that: described man-made feeds are composed of the following components by weight percentage:
Dried tea powder: 15%～20%
Dusty yeast: 5%～10%
Honey: 1%～3%
Vitamin B compound: 0.5%～1%
Sucrose: 1%～5%
Analysis for soybean powder: 5%～10%
Sorbic acid: 0.1～0.5%
Nipalgin: 0.1～0.3%
Agar; 1%～2%
Soil extraction: 20%～30%
Distilled water: surplus.
2. the beautiful line weevil of tea as claimed in claim 1 adult man-made feeds, it is characterized in that: described man-made feeds are composed of the following components by weight percentage: dried tea powder 16%, dusty yeast 6.6%, honey 1.1%, vitamin B compound 0.8%, sucrose 1.4%, analysis for soybean powder 5.4%, sorbic acid 0.1%, nipalgin 0.2%, agar 1.9%, soil extraction 25%, distilled water: surplus.
3. the preparation method of the beautiful line weevil of tea as claimed in claim 1 or 2 adult man-made feeds is characterized in that:
A. the preparation of raw material:
The preparation of dried tea powder: select the ripe tea leaf of giving birth to then through 60 ℃ ± 2 ℃ oven dry, pulverize, cross 60 mesh sieves, make dried tea powder;
The preparation of soil extraction: take the air-dry soil through sieving, this soil organic matter content is not less than 30g/kg, soil thickness 0～20cm, distilled water immersion 2h, swing gently, then be that 0.1g/ml adds the water preparation by concentration, with the speed stirring of 60 times/min, then standing, clarification, supernatant is soil extraction;
The preparation of agar mixed liquor: after in described agar powder, interpolation distilled water is heated to dissolve, be cooled to 65 ℃ ± 2 ℃, add described sorbic acid, described nipalgin, mixed dissolution;
B. described dried tea powder, described analysis for soybean powder and described dusty yeast are mixed;
C. described sucrose, described vitamin B compound and described honey are mixed;
D. the mixture with step B and C preparation mixes;
E. add in step D together with described soil extraction after described agar mixed liquor being cooled to 60 ℃ ± 2 ℃, mix and obtain the beautiful line weevil of tea adult man-made feeds.
4. the preparation method of the beautiful line weevil of tea as claimed in claim 3 adult man-made feeds is characterized in that:
A. the preparation of raw material:
The preparation of dried tea powder: select the ripe tea leaf of giving birth to then through 60 ℃ ± 2 ℃ oven dry, pulverize, cross 60 mesh sieves, make dried tea powder;
The preparation of soil extraction: take the air-dry soil that sieved through 1mm soil, this soil organic matter content is not less than 30g/kg, soil thickness 20cm, distilled water immersion 2h, swing gently, then be that 0.1g/ml adds the water preparation by concentration, with the speed stirring of 60 times/min, then standing, clarification, supernatant is soil extraction
The preparation of agar mixed liquor: after in described agar powder, interpolation distilled water is heated to dissolve, be cooled to 65 ℃, add described sorbic acid, described nipalgin, mixed dissolution;
B. described dried tea powder, described analysis for soybean powder and described dusty yeast are mixed;
C. described sucrose, described vitamin B compound and described honey are mixed;
D. the mixture with step B and C configuration mixes;
E. add in step D together with described soil extraction after described agar mixed liquor being cooled to 60 ℃ ± 2 ℃, mix and obtain the beautiful line weevil of tea adult man-made feeds.
5. the rearing method that adopts the beautiful line weevil of the described tea of claim 1 or 2 adult man-made feeds to raise the beautiful line weevil of tea adult is characterized in that:
(1) gather the beautiful line weevil of tea pupa;
(2) pupa is placed in the vial that fills the thick sandy soil of 4cm～5cm waits to sprout wings, bottleneck seals with gauze, and temperature remains on 26 ℃ ± 1 ℃, relative humidity 80% ± 5%;
(3) adult that sprouts wings is put into fill the new vial of the thick sandy soil of 4cm～5cm, every bottle of raisings is female, each of male imago, regular feeding; Bottleneck seals with gauze, and temperature remains on 26 ℃ ± 1 ℃, relative humidity 80% ± 5%.
6. the rearing method of the beautiful line weevil of tea as claimed in claim 5 adult is characterized in that: the next day with inserting moisturizing in vial after a cotton pellet immersion.
7. the rearing method of the beautiful line weevil of tea as described in claim 5 or 6 adult is characterized in that: carrying one of man-made feeds of broad bean size with cover glass in every 3 days puts into bottle.
8. adopt the method for the beautiful line weevil of the beautiful line weevil of the tea adult man-made feeds artificial feeding tea adult that the described preparation method of claim 3 or 4 makes, it is characterized in that:
(1) gather the beautiful line weevil of tea pupa;
(2) pupa is placed in the vial that fills the thick sandy soil of 4cm～5cm waits to sprout wings, bottleneck seals with gauze, and temperature remains on 26 ℃ ± 1 ℃, relative humidity 80% ± 5%; The next day with inserting moisturizing in vial after a cotton pellet immersion;
(3) adult that sprouts wings is put into the new vial that fills the thick sandy soil of 4cm～5cm, every bottle of raisings is female, each of male imago, carries one of man-made feeds of broad bean size with cover glass in every 3 days to put into a bottle feeding; Bottleneck seals with gauze, and temperature remains on 26 ℃ ± 1 ℃, relative humidity 80% ± 5%, the next day with inserting moisturizing in vial after a cotton pellet immersion.
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| CN103704508A (en) * | 2013-12-19 | 2014-04-09 | 北京林业大学 | Semi-artificial feed for eucryptorrhynchus chinenis and artificial breeding method |
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| CN107969576A (en) * | 2017-12-11 | 2018-05-01 | 四川省农业科学院植物保护研究所 | A kind of man-made feeds of Larva of Mylabris phalerata and its preparation method and application |
| CN110115318A (en) * | 2019-04-09 | 2019-08-13 | 上海师范大学 | A kind of versatility beetle jelly and preparation method thereof |
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| CN103704508A (en) * | 2013-12-19 | 2014-04-09 | 北京林业大学 | Semi-artificial feed for eucryptorrhynchus chinenis and artificial breeding method |
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| CN107969576A (en) * | 2017-12-11 | 2018-05-01 | 四川省农业科学院植物保护研究所 | A kind of man-made feeds of Larva of Mylabris phalerata and its preparation method and application |
| CN107969576B (en) * | 2017-12-11 | 2021-01-19 | 四川省农业科学院植物保护研究所 | Artificial feed for cantharis larvae, and preparation method and application thereof |
| CN110115318A (en) * | 2019-04-09 | 2019-08-13 | 上海师范大学 | A kind of versatility beetle jelly and preparation method thereof |
| CN111149770A (en) * | 2020-01-10 | 2020-05-15 | 乐山师范学院 | Device and method for collecting excrement of bamboo weevils |
| CN111149770B (en) * | 2020-01-10 | 2022-02-01 | 乐山师范学院 | Method for collecting excrement of bamboo weevil |
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| CN103141670B (en) | 2013-12-25 |
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