CN110115250A - A kind of rearing method and larva artificial feed of the full worm state of citrus fruit fly - Google Patents
A kind of rearing method and larva artificial feed of the full worm state of citrus fruit fly Download PDFInfo
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- CN110115250A CN110115250A CN201910389439.0A CN201910389439A CN110115250A CN 110115250 A CN110115250 A CN 110115250A CN 201910389439 A CN201910389439 A CN 201910389439A CN 110115250 A CN110115250 A CN 110115250A
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- 241000255588 Tephritidae Species 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000000384 rearing effect Effects 0.000 title claims abstract description 16
- 241000382353 Pupa Species 0.000 claims abstract description 25
- 239000002689 soil Substances 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 230000001418 larval effect Effects 0.000 claims abstract description 5
- 230000017448 oviposition Effects 0.000 claims abstract description 5
- 239000006188 syrup Substances 0.000 claims abstract description 5
- 235000020357 syrup Nutrition 0.000 claims abstract description 5
- 230000035800 maturation Effects 0.000 claims abstract description 3
- 239000000843 powder Substances 0.000 claims description 18
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 15
- 239000001963 growth medium Substances 0.000 claims description 14
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 13
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 12
- 235000000346 sugar Nutrition 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 229960004793 sucrose Drugs 0.000 claims description 10
- 210000003298 dental enamel Anatomy 0.000 claims description 9
- 241000207199 Citrus Species 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 5
- 239000004334 sorbic acid Substances 0.000 claims description 5
- 229940075582 sorbic acid Drugs 0.000 claims description 5
- 235000010199 sorbic acid Nutrition 0.000 claims description 5
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 4
- 229930091371 Fructose Natural products 0.000 claims description 4
- 239000005715 Fructose Substances 0.000 claims description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 239000011435 rock Substances 0.000 claims description 4
- 239000002023 wood Substances 0.000 claims description 4
- 239000008121 dextrose Substances 0.000 claims description 3
- 235000013681 dietary sucrose Nutrition 0.000 claims description 3
- 235000013601 eggs Nutrition 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 241000006364 Torula Species 0.000 claims description 2
- 239000003610 charcoal Substances 0.000 claims description 2
- 230000005070 ripening Effects 0.000 claims description 2
- 235000020097 white wine Nutrition 0.000 claims description 2
- 241000675108 Citrus tangerina Species 0.000 claims 1
- 239000006071 cream Substances 0.000 claims 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 238000002360 preparation method Methods 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 8
- 102000002322 Egg Proteins Human genes 0.000 abstract description 5
- 108010000912 Egg Proteins Proteins 0.000 abstract description 5
- 210000004681 ovum Anatomy 0.000 abstract description 5
- 241001672694 Citrus reticulata Species 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 6
- 230000019617 pupation Effects 0.000 description 5
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 235000021551 crystal sugar Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 230000005405 multipole Effects 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 240000002900 Arthrospira platensis Species 0.000 description 1
- 235000016425 Arthrospira platensis Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
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- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Feed For Specific Animals (AREA)
Abstract
A kind of rearing method and larva artificial feed of the full worm state of citrus fruit fly, the rearing method, comprising the following steps: (1) larval phase: citrus fruit fly larva is placed in the culture dish equipped with citrus fruit fly larva artificial feed and carries out artificial feeding；(2) it pupates the phase: to citrus fruit fly larval maturation, being placed in feeding soil and pupate；(3) adult stage: the citrus fruit fly adult that pupa is sprouted wings, which is placed in insectary to be raised with syrup to oviposition, enters next generation.The invention also includes described citrus fruit fly larva artificial feeds and preparation method thereof.Using method for breeding of the present invention, citrus fruit fly full worm state breeding cycle can be shortened, the survival rate of larvae conspicuousness of artificial feeding improves, it can be achieved that the complete artificial feeding continual from generation to generation of citrus fruit fly ovum, larva, pupa, adult；The raw material of the larva artificial feed is easy to get, low in cost, and preparation method is simple.
Description
Technical field
The present invention relates to a kind of method for breeding of citrus fruit fly and feeds, and in particular to a kind of full worm state of citrus fruit fly
Rearing method and larva artificial feed.
Background technique
Citrus fruit fly is caused harm with young wormy fruit, since larva is in fruit, is prevented and treated using herbal sprinkling extremely difficult.Adult
Sprout wings free living after being unearthed, supplements the nutrients and mate on orangery periphery, when oviposition enters orangery and lays eggs in fruit, larvae hatch
Afterwards, fruit is endangered.Citrusfruit loses generally 10%~20%, and up to 60%~90% when serious, Hunan Province is due to the big reality of citrus
The harm of fly causes at least 300,000,000 yuan of direct economic loss every year.Citrus fruit fly adult live range it is wide, orchard application prevention and treatment at
Imitate little, larva can not prevent and treat in fruit, and larva, which buries, to pupate, it is also difficult to prevent and treat.It is unified that therefore, it is necessary to a large amount of physical signs
Test worm carry out insect basic research and pest control technical research.But problem is citrus fruit fly pupa time in the natural environment
It is about 6 months, so long breeding cycle undoubtedly significantly limits insect basic research and pest control technical research, needs
Develop that a kind of breeding cycle is short, the high citrus fruit fly ovum of larva survival rate, larva, pupa, the complete generation artificial feeding of adult
Technology.
104095151 A of CN discloses a kind of citrus fruit fly larva meridic diet and preparation method thereof, half people
Work feed is made of the raw material of following proportion: 20~30g of soybean powder, 20~30g of corn flour, 5~10g of sugar, 4~8g of yeast, dimension
Raw 0.3~0.4g of element, 0.05~0.1mL of acid, 10~15g of fresh citrus pulp, 0~0.5g of spirulina powder, fresh orange blossom 8~
12mL, 2~5g of toilet paper, 120~140mL of water, 0.08~0.2g of preservative.Meridic diet formula is complicated, cost of material
It is higher, and without matched rearing method, breeding cycle is also longer.
104431439 A of CN discloses a kind of citrus fruit fly adult man-made feeds and the preparation method and application thereof, the hair
Bright citrus fruit fly adult man-made feeds, are prepared from the following raw materials: 24~36g of brown sugar, yeast extract or/and yeast extract or/
With 8~12g of yeast powder, 200~400g of distilled water.The man-made feeds feed, when application, need to frequently replace cotton core and plastic cup,
Higher cost pollutes environment.
Currently, being had not been reported for citrus fruit fly ovum, larva, pupa, the complete generation artificial diet technique of adult.
Summary of the invention
The technical problem to be solved by the present invention is to overcome drawbacks described above of the existing technology, provide a kind of breeding week
Phase is short, and larva survival rate is high, especially can both realize the full worm state of citrus fruit fly, including citrus fruit fly in room conditions
The rearing method of ovum, larva, pupa, adult complete generation.
The further technical problems to be solved of the present invention are to overcome drawbacks described above of the existing technology, provide a kind of original
Material is easy to get, and is formulated simpler, low in cost, citrus fruit fly larva artificial feed of high survival rate and preparation method thereof.
The technical solution adopted by the present invention to solve the technical problems is as follows: a kind of artificial feeding of the full worm state of citrus fruit fly
The method of supporting, comprising the following steps:
(1) larval phase: citrus fruit fly larva is placed in the culture dish equipped with citrus fruit fly larva artificial feed and carries out people
Work raising；
(2) pupate the phase: citrus fruit fly larva comes to the ripening period, and is placed in feeding soil and pupates；
(3) adult stage: the citrus fruit fly adult after pupa is sprouted wings is placed in insectary, with syrup artificial feeding to entrance of laying eggs
Next generation.
Further, in step (1), the citrus fruit fly larva artificial feed in the culture dish every 5~10 days (preferably
7 days) replace primary, 10~15 DEG C of the cultivation temperature of artificial feeding；
Further, in step (2), after the big trypetid larva of tangerine enters the stage of pupating, 3~5cm of laying is thick in pupa raising ware
Bottom feeding soil, then the citrus fruit fly larva in stage of pupating will be entered and be placed on bottom feeding soil, and spread 2~3cm thickness
Feeding soil covering, controlling initial pupa and arriving the cultivation temperature of mature pupa is 10~25 DEG C, and cultivating humidity is 50%~70%.
Further, in step (2), the feeding soil be sandy soil, wood sawdust and charcoal according to 1: 2: 1 mass ratio mixing and
At.
Further, in step (3), the adult that the maturation pupa is sprouted wings is placed in insectary, at 24~28 DEG C of temperature, relatively
In the environment of 40%~60% artificial feeding of humidity, entered with 2~15% syrup raising citrus fruit fly adult to oviposition next
From generation to generation.
Further, in step (3), the sugar be white sugar, brown sugar, rock sugar, maltose, sucrose, glucose, in fructose extremely
Few one kind.
The present invention further solves technical solution used by its technical problem: a kind of citrus fruit fly larva it is artificial
Feed is prepared from the following raw materials: a kind of man-made feeds of citrus fruit fly larva are prepared from the following raw materials: soybean powder 20~
40g, 80~120g of citrus pulp, 22~50g of sucrose, 3~5g of agar powder, 1.5~5.5g of yeast, 1~2g of sorbic acid, formaldehyde
0.5~1.5mL, 400~600ml of water.
Further, the sucrose is replaced using at least one of brown sugar, rock sugar, maltose, glucose or fructose.
Further, the yeast be yeast extract, yeast powder, torula, dry ferment, brewer's yeast or white wine yeast in extremely
Few one kind.
Further, the man-made feeds of the citrus fruit fly larva, are made in accordance with the following methods:
(a) raw material is weighed by formula, agar powder, dextrose and saccharose is fitted into Enamel jar, addition is equivalent to enamel cylinder volume
1/3~1/2 water, heating are stirred continuously until boiling；Then soybean powder and remaining water are poured into beaker, is stirred
Uniformly, it pours into the Enamel jar boiled, heats, boil again, citrus pulp is added, continues to boil, keep boiling >=5min, obtain
To culture medium；
(b) 65~75 DEG C are cooled to culture medium obtained by step (a), yeast powder is poured into culture medium, and is stirred evenly；
(c) 45~55 DEG C are cooled to culture medium obtained by step (b), sorbic acid and formaldehyde are poured into culture medium, stirred evenly,
Obtain the man-made feeds of citrus fruit fly larva.(1) larval phase: citrus fruit fly larva is placed in equipped with citrus fruit fly larva people
Artificial feeding is carried out in the culture dish of work feed；
The invention has the advantages that: compared with the prior art, using the rearing method of the full worm state of the citrus fruit fly of the present invention,
The breeding cycle of the full worm state of citrus fruit fly substantially shortens, and pupa time foreshortens to 1~2 month, the entire citrus fruit fly generation cycle
4~5 months are reduced, the larva survival rate conspicuousness of artificial feeding improves, and survival rate of larvae can reach 96.7%, percentage of pupation
Up to 97%, it can be achieved that the complete artificial feeding continual from generation to generation of citrus fruit fly ovum, larva, pupa, adult；The children of the present invention
The raw material of worm man-made feeds is easy to get, and formula is simpler, low in cost, and preparation method is simple.
Specific embodiment
Below with reference to embodiment, the invention will be further described.
Chemical reagent used in the embodiment of the present invention is obtained by routine business approach unless otherwise specified.
A kind of man-made feeds Examples 1 to 3 of citrus fruit fly
A kind of man-made feeds Examples 1 to 3 of citrus fruit fly of the present embodiment, is prepared from the following raw materials:
A kind of preparation method Examples 1 to 3 of the man-made feeds of citrus fruit fly
The acquisition methods of the crystal sugar orange pulp: removing pericarp for crystal sugar orange fruit, and taking out tangerine valve and going peel is crystal sugar orange pulp；
The citrus fruit fly larva artificial feed preparation method of the present embodiment, comprising the following steps:
(1) raw material is weighed by formula, agar powder, dextrose and saccharose is fitted into 1L Enamel jar, addition is equivalent to enamel cylinder body
The water of product 1/3, heating are stirred continuously until boiling；Then soybean powder and remaining water are poured into beaker, is stirred
It is even, it pours into the Enamel jar boiled, heats, boil again, citrus pulp is added, continues to boil, keep boiling 5min, trained
Support base；
(2) 75 DEG C are cooled to culture medium obtained by step (1), yeast powder is poured into culture medium, and is stirred evenly；
(3) 50 DEG C are cooled to culture medium obtained by step (2), sorbic acid and formaldehyde is poured into culture medium, stirred evenly, mandarin orange is obtained
The man-made feeds Examples 1 to 3 of the full worm state of the big trypetid of tangerine.
A kind of rearing method Examples 1 to 3 of citrus fruit fly
The citrus fruit fly pupa rearing method of the present embodiment, comprising the following steps: will be from the mandarin orange of citrus fruit fly egg hatching
The big trypetid larva of tangerine, which is placed in the culture dish equipped with citrus fruit fly man-made feeds, carried out artificial feeding, every 7 days replacement citruses
Big trypetid larva artificial feed；After citrus fruit fly larva enters the stage of pupating, the bottom of laying 3cm thickness in pupa raising ware
The citrus fruit fly larva for entering the stage of pupating (river sand: wood sawdust: turf=1: 2: 1), then is placed on bottom and raised by feeding soil
On soil, spread 2cm thickness feeding soil (river sand: wood sawdust: turf=1: 2: 1) cover, then respectively control larvae pupation temperature
For the comparison of 10 DEG C, 15 DEG C, 20 DEG C, 25 DEG C progress percentage of pupations, after initial pupa becomes mature pupa, the incubation temperature for controlling pupa is
23 ± 2 DEG C, hatching humidity is 60%；The adult that mature pupa is sprouted wings is placed in insectary, air-conditioning temperature control, at 25 ± 2 DEG C of temperature,
Under 50% environment of relative humidity, enter next generation with 5% sucrose water raising citrus fruit fly adult to oviposition.
Citrus fruit fly larva artificial feed 1~3 is compared with 1~2 man-made feeds feeding effect of comparative example in 2 embodiment of table
Note: in table 2, every 10 larvas are a repetition, 10 repetitions.Wherein, in the formula such as Zhou Baichun, 30d survival rate: table is raised
Show in long miscellaneous bacteria and in not long miscellaneous bacteria feeding formula larva average viability；Small letter after raising 30d survival rate data in table
It is female different, it indicates to examine through the new multipole difference multiple comparison graph of SPSS Software of Data Statistics Duncan ' s, it is aobvious in 0.05 level difference
It writes."-" indicates that data do not count.
3 embodiment citrus fruit fly pupa artificial feeding different temperatures culture percentage of pupation result data of table
Note: in table 3, every 50 repeat for one.Wherein, total percentage of pupation (%): indicating in each repetition, and larva number of pupating is accounted for for examination
The percentage of larva number；In table, with lowercase difference person after column data, indicate through SPSS Software of Data Statistics Duncan ' s
New multipole difference multiple comparison graph is examined, significant in 0.05 level difference.
Claims (10)
1. a kind of rearing method of the full worm state of citrus fruit fly, which comprises the following steps:
(1) larval phase: citrus fruit fly larva is placed in the culture dish equipped with citrus fruit fly larva artificial feed and carries out people
Work raising；
(2) pupate the phase: citrus fruit fly larva comes to the ripening period, and is placed in feeding soil and pupates；
(3) adult stage: the citrus fruit fly adult after pupa is sprouted wings is placed in insectary, with syrup artificial feeding to entrance of laying eggs
Next generation.
2. the rearing method of the full worm state of citrus fruit fly according to claim 1, which is characterized in that in step (1), institute
State that citrus fruit fly larva artificial feed in culture dish is primary every replacement in 5~10 days, the cultivation temperature 10 of artificial feeding~
15℃。
3. the rearing method of the full worm state of citrus fruit fly according to claim 1 or claim 2, which is characterized in that step (2)
In, after the big trypetid larva of the tangerine enters the stage of pupating, the bottom feeding soil of laying 3~5cm thickness in pupa raising ware, then will be into
The citrus fruit fly larva for entering the stage of pupating is placed on bottom feeding soil, spreads the feeding soil covering of 2~3cm thickness, control is just
The cultivation temperature of beginning pupa to mature pupa is 10~25 DEG C, and cultivating humidity is 50%~70%.
4. the rearing method of citrus fruit fly described according to claim 1~one of 3, which is characterized in that step (2)
In, the feeding soil is that sandy soil, wood sawdust and charcoal are mixed according to 1: 2: 1 mass ratio.
5. the rearing method of the full worm state of citrus fruit fly described according to claim 1~one of 4, which is characterized in that step
(3) in, the adult that the maturation pupa is sprouted wings is placed in insectary, and at 24~28 DEG C of temperature, relative humidity 40%~60% is manually raised
Under feeding environment, enter next generation with 2~15% syrup raising citrus fruit fly adult to oviposition.
6. the rearing method of the full worm state of citrus fruit fly described according to claim 1~one of 4, which is characterized in that step
(3) in, the sugar is at least one of white sugar, brown sugar, rock sugar, maltose, sucrose, glucose, fructose.
7. a kind of man-made feeds of the citrus fruit fly larva as described in one of claim 1~6, which is characterized in that by following original
Material is made: 20~40g of soybean powder, 80~120g of citrus pulp, 22~50g of sucrose, 3~5g of agar powder, 1.5~5.5g of yeast,
1~2g of sorbic acid, 0.5~1.5mL of formaldehyde, 400~600ml of water.
8. the man-made feeds of citrus fruit fly larva according to claim 7, which is characterized in that the sucrose is using red
At least one of sugar, rock sugar, maltose, glucose or fructose are replaced.
9. the man-made feeds of citrus fruit fly larva according to claim 7 or 8, which is characterized in that the yeast is ferment
At least one of female cream, yeast powder, torula, dry ferment, brewer's yeast or white wine yeast.
10. the man-made feeds of citrus fruit fly larva according to one of claim 7~9, which is characterized in that according to lower section
Method is made:
(a) raw material is weighed by formula, agar powder, dextrose and saccharose is fitted into Enamel jar, addition is equivalent to enamel cylinder volume
1/3~1/2 water, heating are stirred continuously until boiling；Then soybean powder and remaining water are poured into beaker, is stirred
Uniformly, it pours into the Enamel jar boiled, heats, boil again, citrus pulp is added, continues to boil, keep boiling >=5min, obtain
To culture medium；
(b) 65~75 DEG C are cooled to culture medium obtained by step (a), yeast powder is poured into culture medium, and is stirred evenly；
(c) 45~55 DEG C are cooled to culture medium obtained by step (b), sorbic acid and formaldehyde are poured into culture medium, stirred evenly,
Obtain the man-made feeds of citrus fruit fly larva.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111316959A (en) * | 2020-03-25 | 2020-06-23 | 广西壮族自治区农业科学院 | Indoor propagation method for bactrocera dorsalis |
| CN114868708A (en) * | 2022-04-15 | 2022-08-09 | 中南大学 | Breeding and domesticating method for Boettcherisca peregrina |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640867A (en) * | 2012-04-28 | 2012-08-22 | 中国热带农业科学院环境与植物保护研究所 | Artificial feed for feeding fruit fly and method for feeding fruit fly |
| CN103222448A (en) * | 2013-02-05 | 2013-07-31 | 东莞市盛唐化工有限公司 | Breeding method of bactrocera cucurbitae |
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