CN104095151A - Semi-artificial feed for Bactrocera minax larvae and preparation method thereof - Google Patents
Semi-artificial feed for Bactrocera minax larvae and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a semi-artificial feed for Bactrocera minax larvae and a preparation method thereof. The semi-artificial feed is composed of the following raw materials: 20-30g of soybean powder, 20-30g of corn powder, 5-10g of sugar, 4-8g of yeast, 0.3-0.4g of vitamin, 0.05-0.1mL of acid, 10-15g of fresh citrus flesh, 0-0.5g of spirulina powder, 8-12mL of fresh citrus juice, 2-5g of toilet paper, 120-140mL of water, and 0.08-0.2g of a preservative. The invention also includes the preparation method of the semi-artificial feed for Bactrocera minax larvae. The semi-artificial feed provided by the invention has the advantages of easily available formula materials, low cost and simple preparation method, and can feed Bactrocera minax larvae in large quantities.
Description
Technical field
The present invention relates to a kind of meridic diet and preparation method thereof, especially relate to a kind of citrus fruit fly larva meridic diet and preparation method thereof.
Background technology
Citrus fruit fly
bactrocera minax(Enderlein) belonging to Diptera (Diptera) Tephritidae (Tephritidae), is the crushing insect of oranges and tangerines, and this worm is mainly distributed in the oranges and tangerines producing regions such as Hunan, Hubei, Sichuan, Chongqing, Yunnan, Guizhou in China.Within 1 year, there is a generation in this worm, it is laid eggs in citrusfruit, larva is bored in moth tangerine fruit taking tangerine lobe pulp as food, cause the underdone first Huang of wormed fruit to come off in advance, lose edibility, the about 20-30% of fruit loss late, can reach 70%-80% so that 100% when production loss is serious, become the first insect pest of China's Orange Producing, the situation is tense in prevention and control.Research to citrus fruit fly at present, is mainly based upon Natural Population or utilizes natural feed (citrusfruit) to raise and study the aspects such as its biological characteristics, occurrence characteristic, behavior and prophylactico-therapeutic measures.And carry out the artificial feeding of citrus fruit fly, optimize citrus fruit fly larva artificial feeding technology, setting up stable indoor population, provide the health of a large amount of growth neat and consistent for examination worm source, is the basis of further carrying out citrus fruit fly Physiology and biochemistry, science of heredity, toxicology and study on prevention thereof.After the man-made feeds formula of starting calliphora vomitoria in 1908, the research of insect artificial feeding grows up gradually, and up to now, existing 1000 various insects can be raised and breed by using artificial feed both at home and abroad.1956, for Finney, the larva artificial feed of fresh carrot, sucrose, torula, antibiotic composition was successfully fed citrus fruit fly.A lot of scholars, through years of researches, can use the mass rearing citrus fruit flies such as corn flour, sugar, yeast, Mediterranean fruitfly and melon trypetid etc. at present.But about citrus fruit fly man-made feeds, up to the present, only there are one section of report about its adult man-made feeds (Wang little Jiao etc. " impact of man-made feeds on citrus fruit fly Life of Adult and reproductive characteristic ", see hubei agricultural science, 2012,51(11): 2222-2224), about its larva artificial feed formula and artificial feeding technology, there is not yet so far any report.
Summary of the invention
The technical problem to be solved in the present invention is, provides a kind of material to be easy to get, preparation method is simple, cost is low, citrus fruit fly larva meridic diet that can mass rearing citrus fruit fly and preparation method thereof.
The technical scheme that the present invention solves its technical problem employing is:
The present invention's citrus fruit fly larva meridic diet, is made up of the raw material of following proportioning: analysis for soybean powder 20～30g, corn flour 20～30g, sugar 5～10g, yeast 4～8g, vitamin 0.3～0.4g, acid 0.05～0.1mL, fresh oranges and tangerines pulp 10～15g, spirulina powder 0～0.5g, fresh orange blossom 8～12mL, toilet paper 2～5g, water 120～140mL, anticorrisive agent 0.08～0.2g;
Described sugar can be at least one in white sugar, brown sugar, rock sugar, maltose, sucrose, glucose, preferably white sugar;
Described yeast can be yeast extract, dusty yeast, torula, brewer's yeast, white wine yeast or dry ferment, preferred yeast cream;
Described vitamin can be B B-complex, vitamin C (ascorbic acid) or 21 Jin Weita, preferably B B-complex;
Described acid can be at least one in hydrochloric acid, citric acid, malic acid, tartaric acid, oxalic acid, butanedioic acid, lactic acid, formic acid, acetic acid, butyric acid, preferably hydrochloric acid; The pH that adds acid 0.05～0.1mL regulator solution, is faintly acid;
Described fresh oranges and tangerines can be bitter orange, sweet orange, navel orange, crystal sugar orange, mandarin orange, Pon mandarin orange, red tangerine, shaddock, lemon, citron or Buddha's hand, preferably navel orange;
Described water can be distilled water, running water, mineral water, pure water or distilled water, preferably distilled water;
Described anticorrisive agent can be benzoic acid, at least one in hydroxy-methylbenzene, Sodium Benzoate, sorbic acid, potassium sorbate, calcium propionate, sodium propionate, methyl p-hydroxybenzoate, dehydroactic acid, sodium Diacetate, nitrate, nitrite, sodium lactate, sodium propionate, preferably benzoic acid and the mixture that is 1:0.8～1.2 to hydroxy-methylbenzene mass ratio;
Described corn flour, vitamin, sugar and spirulina are commercially available, and vitamin and spirulina grind to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
Described citrus fruit fly larva also can add agar with meridic diet, to utilize moulding;
Described toilet paper usable fibers element replaces.
The acquisition methods of described fresh oranges and tangerines pulp and fresh orange blossom: fresh citrusfruit is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh oranges and tangerines pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or oranges and tangerines are half-and-half cut to directly extruding, the juice of extrusion filters with 60 order gauzes, obtains filtrate and is fresh orange juice.
The preparation method of meridic diet for the present invention's citrus fruit fly larva, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20～30g, corn flour 20～30g, sugar 5～10g, yeast 4～8g, vitamin 0.3～0.4g, acid 0.05～0.1mL, fresh oranges and tangerines pulp 10～15g, spirulina powder 0～0.5g, fresh orange blossom 8～12mL, toilet paper 2～5g, water 120～140mL, anticorrisive agent 0.08～0.2g;
(2) analysis for soybean powder weighing up, corn flour and sugar are mixed;
(3) with beaker water intaking, heating, puts into subsequently and weighs the toilet paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder, corn flour and the sugar mixture that mix through step (2) are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 48～52 DEG C, admix rapidly spirulina powder, vitamin, yeast, fresh oranges and tangerines pulp, fresh orange blossom, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add acid and anticorrisive agent, fully stir evenly.
The present invention's citrus fruit fly larva meridic diet, with a certain amount of analysis for soybean powder, corn flour, sugar, yeast, vitamin, acid, fresh oranges and tangerines pulp, fresh orange blossom, spirulina, water and anticorrisive agent, can successfully raise citrus fruit fly larva indoor, this meridic diet formula materials is easy to get, cost is low, preparation method is simple, can mass rearing citrus fruit fly larva.
Detailed description of the invention
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.The amendment that the inventive method, step or condition are made or replacement, all belong to protection scope of the present invention.
Raw material used in embodiment, unless otherwise indicated, is commercial goods.
Example I
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 20g, white sugar 5g, yeast extract 4g, B B-complex 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 10g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 20g, white sugar 5g, yeast extract 4g, B B-complex 0.3g, hydrochloric acid 0.05mL, fresh navel orange pulp 10g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Example II
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 30g, corn flour 20g, white sugar 5g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid (concentration of hydrochloric acid is 36wt%) 0.1mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 30g, corn flour 20g, white sugar 5g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid 0.1mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g;
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
EXAMPLE III
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 30g, corn flour 20g, white sugar 10g, yeast extract 8g, B B-complex 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 30g, corn flour 20g, white sugar 10g, yeast extract 8g, B B-complex 0.3g, hydrochloric acid 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
EXAMPLE IV
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 20g, white sugar 10g, yeast extract 8g, B B-complex 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.1mL, fresh navel orange pulp 10g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 20g, white sugar 10g, yeast extract 8g, B B-complex 0.4g, hydrochloric acid 0.1mL, fresh navel orange pulp 10g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
EXAMPLE V
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 30g, corn flour 30g, white sugar 5g, yeast extract 8g, B B-complex 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 10g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 30g, corn flour 30g, white sugar 5g, yeast extract 8g, B B-complex 0.4g, hydrochloric acid 0.05mL, fresh navel orange pulp 10g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Example VI
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 30g, white sugar 5g, yeast extract 8g, B B-complex 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.1mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 30g, white sugar 5g, yeast extract 8g, B B-complex 0.3g, hydrochloric acid 0.1mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 52 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Example VII A
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 48 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Example VII A I
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 30g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.1mL, fresh navel orange pulp 10g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 30g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.3g, hydrochloric acid 0.1mL, fresh navel orange pulp 10g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Example I X
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 15g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.3g, hydrochloric acid 0.05mL, fresh navel orange pulp 15g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Embodiment X
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 15g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid 0.05mL, fresh navel orange pulp 15g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Embodiment XI
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.1g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.1g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Embodiment XII
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.2g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.2g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Embodiment XIII
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.3g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.3g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Embodiment XIV
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.4g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Embodiment XV
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.5g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
Described corn flour, B B-complex, white sugar are commercially available, and B B-complex grinds to form powdery;
Described analysis for soybean powder is commercially available soya bean and breaks into powdery;
The acquisition methods of described fresh navel orange pulp and fresh naval orange juice: fresh Navel Orange Fruits is removed to pericarp, take out tangerine lobe and remove endocarp, obtain fresh navel orange pulp; The tangerine lobe taking out is wrapped up with 60 order gauzes, extruding, or navel orange is half-and-half cut to directly extruding, and the juice of extrusion filters with 60 order gauzes), obtain filtrate and be fresh navel orange fruit juice.
The preparation method of meridic diet for the citrus fruit fly larva of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, corn flour 30g, white sugar 10g, yeast extract 4g, B B-complex 0.4g, hydrochloric acid 0.05mL, fresh navel orange pulp 15g, spirulina powder 0.5g, fresh naval orange juice 10mL, napkin paper 3g, distilled water 120mL, anticorrisive agent 0.12g(benzoic acid and to the each 0.06g of hydroxy-methylbenzene);
(2) analysis for soybean powder weighing up, corn flour and white sugar are mixed;
(3) get distilled water with beaker, heating, puts into subsequently and weighs the napkin paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder mixing through step (2), corn flour and white sugar mixture are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 50 DEG C, admix rapidly spirulina powder, B B-complex, yeast extract, fresh navel orange pulp, fresh naval orange juice, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add hydrochloric acid and anticorrisive agent, fully stir evenly, to obtain final product.
Feed experiment:
(every 10 is a repetition to feed citrus fruit fly larva with embodiment of the present invention I～XV gained meridic diet, repeat 6～15 times), the long average increasing amount of body of 10d after the average survival rate of 10d, raising after the larva number that each embodiment feeds and raising, and average always percentage of pupation is in table 1.
Table 1 example I～XV gained meridic diet is fed the result data of citrus fruit fly larva
Note: in table 1, every 10 is a repetition.Wherein,
Average survival rate (%): represent in each repetition, survival larva number accounts for the percentage for examination larva number;
The long average increasing amount of body (mm/ head): represent in each repetition the long increment of average body (millimeter/head) of survival larva;
Average total percentage of pupation (%): represent in each repetition, the borer population of pupating accounts for the percentage for examination larva number;
In table, with lowercase difference person after column data, represent through the poor multiple comparison method inspection of the new multipole of SPSS Software of Data Statistics Duncan ' s, remarkable at 0.05 level difference.
Claims (9)
1. a citrus fruit fly larva meridic diet, it is characterized in that, formed by the raw material of following proportioning: analysis for soybean powder 20～30g, corn flour 20～30g, sugar 5～10g, yeast 4～8g, vitamin 0.3～0.4g, acid 0.05～0.1mL, fresh oranges and tangerines pulp 10～15g, spirulina powder 0～0.5g, fresh orange blossom 8～12mL, toilet paper 2～5g, water 120～140mL, anticorrisive agent 0.08～0.2g;
Described anticorrisive agent is benzoic acid, at least one in hydroxy-methylbenzene, Sodium Benzoate, sorbic acid, potassium sorbate, calcium propionate, sodium propionate, methyl p-hydroxybenzoate, dehydroactic acid, sodium Diacetate, nitrate, nitrite, sodium lactate, sodium propionate.
2. citrus fruit fly larva meridic diet according to claim 1, is characterized in that, described sugar is at least one in white sugar, brown sugar, rock sugar, maltose, sucrose, glucose.
3. citrus fruit fly larva meridic diet according to claim 1 and 2, is characterized in that, described yeast is yeast extract, dusty yeast, torula, dry ferment, brewer's yeast or white wine yeast.
4. citrus fruit fly larva meridic diet according to claim 1 and 2, is characterized in that, described vitamin is B B-complex, vitamin C or 21 Jin Weita.
5. citrus fruit fly larva meridic diet according to claim 1 and 2, is characterized in that, described acid is at least one in hydrochloric acid, citric acid, malic acid, tartaric acid, oxalic acid, butanedioic acid, lactic acid, formic acid, acetic acid, butyric acid.
6. citrus fruit fly larva meridic diet according to claim 1 and 2, is characterized in that, described fresh oranges and tangerines are bitter orange, sweet orange, navel orange, crystal sugar orange, mandarin orange, Pon mandarin orange, red tangerine, shaddock, lemon, citron or Buddha's hand.
7. citrus fruit fly larva meridic diet according to claim 1 and 2, is characterized in that, described water is distilled water, running water, mineral water, pure water or distilled water.
8. citrus fruit fly larva meridic diet according to claim 1 and 2, is characterized in that, described anticorrisive agent is benzoic acid and the mixture that is 1:0.8～1.2 to hydroxy-methylbenzene mass ratio.
9. a method of preparing the citrus fruit fly larva meridic diet as described in one of claim 1-8, is characterized in that, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20～30g, corn flour 20～30g, sugar 5～10g, yeast 4～8g, vitamin 0.3～0.4g, acid 0.05～0.1mL, fresh oranges and tangerines pulp 10～15g, spirulina powder 0～0.5g, fresh orange blossom 8～12mL, toilet paper 2～5g, water 120～140mL, anticorrisive agent 0.08～0.2g;
(2) analysis for soybean powder weighing up, corn flour and sugar are mixed;
(3) with beaker water intaking, heating, puts into subsequently and weighs the toilet paper of getting well and tearing up, and stirs, until water boiling while boiling;
(4) analysis for soybean powder, corn flour and the sugar mixture that mix through step (2) are poured in step (3) gained boiling water, stirred evenly, obtain culture medium;
(5) by naturally cooling step (4) gained culture medium, in the time that temperature is down to 48～52 DEG C, admix rapidly spirulina powder, vitamin, yeast, fresh oranges and tangerines pulp, fresh orange blossom, stir evenly;
(6) after the culture medium of processing through step (5) is completely cooling, add acid and anticorrisive agent, fully stir evenly.
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| CN110024748A (en) * | 2019-03-11 | 2019-07-19 | 安徽师范大学 | A kind of Drosophila medium and its preparation and method for filling |
| CN110115250A (en) * | 2019-05-10 | 2019-08-13 | 湖南农业大学 | A kind of rearing method and larva artificial feed of the full worm state of citrus fruit fly |
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