CN102987165A

CN102987165A - South Asia trypetid larva artificial feed

Info

Publication number: CN102987165A
Application number: CN2012105771374A
Authority: CN
Inventors: 季清娥; 陈家骅; 潘靖; 洪静芳; 郑概英
Original assignee: Fujian Agriculture and Forestry University
Current assignee: Fujian Agriculture and Forestry University
Priority date: 2012-12-27
Filing date: 2012-12-27
Publication date: 2013-03-27

Abstract

The invention relates to the field of artificial feeding of insects and particularly relates to an artificial feed for South Asia trypetid larvae. The artificial feed for South Asia trypetid larvae comprises the following raw materials by weight percent: 25-30% of wheat bran, 9-11% of cane sugar, 3.5-6.0% of beer yeast, 0.1-0.2% of sodium benzoate, 0.1-0.2% of methyl parahydroxybenzoats and the balance of water. The artificial feed for South Asia trypetid larvae is low cost and wide in sources of raw materials and can meet the growth and development requirement of the South Asia trypetid larvae. Thus, lots of healthy and regularly developed insect sources for test can be supplied for the research and preventive treatment of physiology, genetics, toxicology and the like.

Description

A kind of Bactrocera tau larva artificial feed

Technical field

The present invention relates to insect artificial feeding field, be specifically related to a kind of Bactrocera tau larva artificial feed.

Background technology

Bactrocera tau Bactrocera tau(walker) belong to Diptera Diptera, Tephritidae Tephritidae, Bactrocera BactroceraMacquart, can endanger the 70 various fruits vegetables such as the melon and citrus of 13 kinds of sponge gourd, pumpkin, cucumber, balsam pear, cucurbita pepos etc., mango, tomato, pawpaw guava, a kind of important fruit, vegetable-crop pest-insect, at present in China Taiwan and provinces and regions, 14 continents distribution is arranged, be on the rise in the harm of China south China, southwest, Central China.

The artificial feeding of Bactrocera tau larva still mainly adopts at present the fruit of its natural host plant such as sponge gourd, cucumber, pumpkin, balsam pear etc. to raise, but raise the restriction that Bactrocera tau is subject to host, season with natural feed, cost is high, can not satisfy the needs of every research; On the other hand, owing to using in a large number of agricultural chemicals, the control of the difficult quality of every batch natural host fruit, thereby be unable to supply the experiment unified, health of a large amount of physiological standards and use worm.

Summary of the invention

A kind of Bactrocera tau larva artificial feed provided by the present invention, cost is low, raw material sources are wide, can satisfy the needs that grow of Bactrocera tau larva.

The present invention adopts following scheme:

A kind of Bactrocera tau larva artificial feed, its raw material consists of: 25～30% wheat bran, 9～11% sucrose, 3.5～6.0% brewer's yeasts, 0.1～0.2% Sodium Benzoate, 0.1～0.2% methyl p-hydroxybenzoate, surplus are water.

More specifically, comprise 750～850g wheat bran, 250～350g sucrose, 100～200g brewer's yeast, 2.5～3.5g Sodium Benzoate, 3～4g methyl p-hydroxybenzoate and 1.5～2L water in every part of man-made feeds.

As preferably, every part of man-made feeds contain 800g wheat bran, 300g sucrose, 150g yeast, 3g Sodium Benzoate, 3.5g methyl p-hydroxybenzoate and 1.5L water.First 3g Sodium Benzoate, 3.5g methyl p-hydroxybenzoate are dissolved with a small amount of warm water, then mix with other compositions, be tiled in the larva raising dish after adding 1.5L running water is mixed thoroughly.

Beneficial effect:

The Bactrocera tau larva artificial feed that the present invention adopts such scheme to provide, cost is low, raw material sources are wide, can reduce the artificial feeding cost of Bactrocera tau, and can fully satisfy the needs that grow of Bactrocera tau larva, the research and the preventing and controlling that can be the aspects such as physiology, science of heredity, toxicology provide a large amount of health, neat the supplying of growth to try the worm source.

The specific embodiment

Embodiment 1 most preferred embodiment

The artificial feeding step of Bactrocera tau comprises that adopting ovum → ovum hatches into larva → larva artificial feeding → larvae pupation → pupa and sprout wings into adult → imago breeding etc.

One, man-made feeds preparation

Fill a prescription according to the Bactrocera tau larva artificial feed, the 800g wheat bran, 300g sucrose, the 150g beer waste yeast that weigh up are poured in larger plastic tub or the bucket, taking by weighing 3g Sodium Benzoate, 3.5g methyl p-hydroxybenzoate pours in the little container (plastic cup or glass) and to pour in the said vesse after dissolving with a small amount of warm water, add the 1.5L running water, after stirring, pour larva raising dish (plastic material into, 49 cm X32 cm X5cm), every dish is poured 2 parts of man-made feeds into, about 5.5kg, manual decurl, and at centre position layer overlay toilet paper, for subsequent use.

Two, adopt ovum, spread ovum, pupate

The Bactrocera tau Adult Development during to sexal maturity (about 20d sexal maturity behind the adult eclosion) carry out mating, lay eggs.The ovum artificial collection method is to put into the artificial feeding cage with adopting egg apparatus, take out after about 5-6 hour and adopt egg apparatus, water is gone out ovum from adopt egg apparatus, the ovum amount that arrives with the graduated cylinder measurement collection, the about 6ml ovum of every dish feed Pan Pu, ovum is tiled on the toilet paper, with the watering can watering ovum is roughly disperseed, then rearing tray is placed on the springtail frame, springtail frame below is placed with sand table, at 26 ± 1 ℃, raise under RH70 ± 10% condition, after about 1 week, mature larva can be grown in sand table from the rearing tray introskip and pupated, and behind about 4d pupa is sifted out from sand.

Three, quality-monitoring

Raised for 3 generations continuously with man-made feeds, the host who adopts Bactrocera tau to have a liking for most simultaneously----sponge gourd breeding grub compares (CK), to per generation the quality index such as egg hatching rate, survival rate of larvae, pupa weight, eclosion rate, the rate that flies out monitor.

Egg hatching rate:

Get at random 100 on each generation and CK offspring ovum, place on the moistening filter paper of culture dish, place under (25 ± 1) ℃, RH 65% ± 5%, the L:D=12:12 condition, check unhatched ovum at microscopically after 48 hours and count L, calculate egg hatching rate H%, H%=(100-L)/100 * 100.Three groups of repetitions are established in experiment.

Survival rate of larvae:

Get at random each generation and CK and just hatched 100 of larvas, place respectively in the plastics tray that man-made feeds and sponge gourd are housed, again vinyl disc is placed the bottom to be covered with in the plastic tub of fine sand.Under (25 ± 1) ℃, RH 65% ± 5%, L:D=12:12 condition, raise, jump in the sand and the rear sieve of all pupating is husky, check pupa quantity P alive, calculating survival rate of larvae S%, S%=P/100 * 100 until larva is aging.Three groups of repetitions are established in experiment.

Pupa is heavy: get at random 100 of the pupas in 10 days pupa ages, weigh, average;

Eclosion rate, rate flies out: at 1.2 m * 0.6 m * 1.2 m dependent insect cage (aluminum alloy frames, the nylon grenadine) put 5 diameter 10 ㎝ in, high 20 ㎝ inwalls scribble talcous black plastic cylinder, put into the pupa in 100 10 days pupa ages in every, place under (25 ± 1) ℃, RH 65% ± 5%, the L:D=12:12 condition, the respectively dead pupa quantity A in the recording drum, partly sprout wings quantity B, lopsided trypetid quantity C, the normal but trypetid quantity D of the cylinder of failing to fly out after 6 days are calculated respectively eclosion rate E%, the rate that flies out F%.

E%＝(100-A-B)/100×100

F%＝(100-A-B-C-D)/100×100。

Four, results and analysis

Table 1 Bactrocera tau man-made feeds are raised the quality index of raising with sponge gourd and are compared

Figure 2012105771374100002DEST_PATH_IMAGE001

Annotate: data are average ± standard error in the table.Adopt the Tukey multiple ratio, with different letter representation significant differences behind the column data, wherein lowercase be illustrated in 0.05 ( P＜0.05) significant difference on the level, capitalization be illustrated in 0.01 ( P＜0.01) significant difference on the level.

As can be seen from Table 1, man-made feeds are raised the incubation rate and contrast there was no significant difference of the ovum in 3 generations continuously, and all more than 90%; There is not significant difference in survival rate of larvae with contrast yet, all greater than 80%.

The pupa weight average is heavy the heaviest greater than the pupa of contrast and 1st generation, has significant difference with 2nd generation and contrast, but different not remarkable with the 3rd generation-difference; 2nd, 3 generations and contrast poor between without significant difference.

1st generation eclosion rate and the rate that flies out are the highest, and the eclosion rate in the 2nd, 3 generations, the rate that flies out all slightly are lower than contrast, but the eclosion rate in the 1st, 2,3 generations and the difference that flies out that there are no significant between rate and the contrast.

The result show Bactrocera tau quality that man-made feeds raise continuously and host's raising without significant difference, can be with this larva artificial feed mass rearing Bactrocera tau.

Embodiment 2(following examples are in order to support better claim)

Comprise 750g wheat bran, 250g sucrose, 150g brewer's yeast, 2.5g Sodium Benzoate, 3.5g methyl p-hydroxybenzoate and 1.5L water in every part of man-made feeds.

First Sodium Benzoate, methyl p-hydroxybenzoate are dissolved with a small amount of warm water, then mix with other compositions, be tiled in the larva raising dish after adding 1.5L running water is mixed thoroughly.

Embodiment 3

Comprise 850g wheat bran, 350g sucrose, 200g brewer's yeast, 3.5g Sodium Benzoate, 4g methyl p-hydroxybenzoate and 2L water in every part of man-made feeds.

First Sodium Benzoate, methyl p-hydroxybenzoate are dissolved with a small amount of warm water, then mix with other compositions, be tiled in the larva raising dish after adding 2L running water is mixed thoroughly.

Claims

1. Bactrocera tau larva artificial feed, it is characterized in that: the raw material of described Bactrocera tau larva artificial feed consists of: 25～30% wheat bran, 9～11% sucrose, 3.5～6.0% brewer's yeasts, 0.1～0.2% Sodium Benzoate, 0.1～0.2% methyl p-hydroxybenzoate, surplus are water.