CN106070077B - Method for breeding cordyceps sinensis host larvae in turns - Google Patents

Method for breeding cordyceps sinensis host larvae in turns Download PDF

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CN106070077B
CN106070077B CN201610519952.3A CN201610519952A CN106070077B CN 106070077 B CN106070077 B CN 106070077B CN 201610519952 A CN201610519952 A CN 201610519952A CN 106070077 B CN106070077 B CN 106070077B
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radix
raising
larva
larvae
raised
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CN106070077A (en
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李文佳
张宗耀
李全平
吕延华
魏再鸿
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YICHANG SHANCHENGSHUIDU CORDYCEPS Co.,Ltd.
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Guangdong HEC Pharmaceutical
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

Abstract

The invention provides a method for breeding cordyceps sinensis host larvae in turns. The method mainly comprises the following steps that 1, the two-year-old larvae are bred through the mixture of serpentgrass, bistorta macrophylla and rheum pumilum; 2, the 3-year-old, 4-year-old and 5-year-old larvae are bred through the mixture of salvia miltiorrhiza, stachys sieboldii and rheum pumilum; 3, the larvae getting into the final age are bred through the mixture of carrots, jerusalem artichoke, rheum pumilum and salvia miltiorrhiza. By means of cyclic breeding, the host larvae can absorb balanced nutrition at the whole growth stage, the nutrition lack disease of the larvae is solved, it can be ensured that subsequent cordyceps sinensis has high quality, and the growth period of the larvae is shortened.

Description

A kind of method that host of Cordyceps sinensis larva is raised in rotation
Technical field
The present invention relates to a kind of method that host of Cordyceps sinensis larva is raised in rotation.
Background technology
, due to being grown in 3000~5000m of height above sea level Qinghai-xizang Plateau Region, natural parasitic rate is low, to life cycle for Cordyceps Border condition is extremely harsh, so resource itself is very limited, in recent years due to the huge market demand, price Continued causes A large amount of blindly unreasonable excavations, cause wild resource to reduce increasingly, and yield declines year by year, the people of host of Cordyceps sinensis larval maturation Work breeding system is the technical problem of those skilled in the art's urgent need to resolve.
Fresh food materials common manifestation about artificial breeding's Vespertilio moth larvae is unification now, for example, only use Radix Dauci Sativae, pearl Bud knotweed etc. individually raises Vespertilio moth larvae, also has and is raised (referring to China specially using Radix Dauci Sativae, Chinese artichoke, Potentilla anserina mixing Profit, publication number:CN 102813081A), but all have ignored Vespertilio moth larvae segment 7 ages (have small part larva to have the 8th Age), each instar larvae takes food hobby and differs, and easily causes the nutritional deficiency of host larva.
It is to take food hobby for each worm age section of Hepialus armorieanus Oberthur that rotation is raised, and feeds different foods to different worm instar larvaes, makes The whole growth stage of host larva can absorb nutrition in a balanced way, solve Larval foods disappearance disease.And have no that the country has manually at present Rotation raise Vespertilio moth larvae report, only the luxurious feeding habits of Hepialus larva and take food quantity research report (Zhang Deli etc., bat children The luxurious feeding habits of worm and quantity research is taken food, southwest agricultural journal, 6 phases of volume 27 in 2014,2732-2734.), do not refer to that rotation is raised.
The content of the invention
Deficiency of the present invention according to above-mentioned background technology, takes food hobby for each worm age section of Hepialus armorieanus Oberthur, and invention is a kind of to be adapted to The method that the rotation of host of Cordyceps sinensis larva is raised.Larva is selected to like best using the hobby that takes food of each worm age section Vespertilio moth larvae Feedstuff raised, i.e., being raised by rotation enables the whole growth stage of host larva to absorb nutrition in a balanced way, solves larva Nutritional deficiency disease, also ensures that follow-up Cordyceps are possessed of good qualities;A kind of plateau feedstuff Radix Salviae Miltiorrhizae is filtered out particularly, first The raising for Vespertilio moth larvae is reported, the selection of Hepialus armorieanus Oberthur host-feeding is enriched.
The invention provides a kind of method that host of Cordyceps sinensis larva is raised in rotation, comprises the following steps:
(1) 1,2 instar larvaes are raised with the mixing of Polygonum viviparum L., motion eccentric and Radix et Rhizoma Rhei;
(2) continue the mixing of 3,4,5 instar larvae Radix Salviae Miltiorrhizaes, Rhizoma Humatae Tyermanni and Radix et Rhizoma Rhei to raise;
(3) larva into last age is raised with the mixing of Radix Dauci Sativae, Jerusalem artichoke, Radix et Rhizoma Rhei and Radix Salviae Miltiorrhizae.
In some embodiments, methods described is raised using the raising frame equipped with sterilization upland soil.
In some embodiments, sterilization upland soil THICKNESS CONTROL is:Feedstuff is gone to the bottom floor height original soil thickness at least 3cm, raises Material upper epidermis plateau soil thickness 3-6cm.
In some embodiments, methods described is raised using the raising frame equipped with sterilization upland soil, and it is high to sterilize Original soil THICKNESS CONTROL is:Feedstuff is gone to the bottom floor height original soil thickness at least 3cm, feedstuff upper epidermis plateau soil thickness 3-6cm.
In some embodiments, the humid control of sterilization upland soil is in 45%-65%.
In some embodiments, between the raising that methods described is adopted, purity requirements are for 1,000,000 grades.
In some embodiments, the temperature of larva raising, humidity, percentage of possible sunshine simulate 1981 completely to 2010 Tibetans The about 205 meteorological station moon of plateau and periphery value meteorological data of that month meansigma methodss, and day and night temperature will refer to changes in temperature season respectively Historical data.
In some embodiments, the weather conditions of larva raising are simulation Qinghai-Tibet plateau climate.
In some embodiments, the temperature controlling range of larva raising is 2 DEG C -14 DEG C of ﹣.
In some embodiments, the humid control scope of larva raising is 30%-90%.
In some embodiments, the temperature controlling range of larva raising is 2 DEG C -14 DEG C of ﹣, and humid control scope is 30%-90%.
In some embodiments, the quality of the Polygonum viviparum L., motion eccentric and Radix et Rhizoma Rhei used by 1,2 instar larvaes is raised in mixing Ratio is (10%~50%):(10%~50%):(10%~50%), preferred mass ratio are (20%~40%): (20%~40%):(20%~40%), preferred mass ratio are equal proportion, and its ratio sum is equal to 100%.
In some embodiments, the quality of the Radix Salviae Miltiorrhizae, Rhizoma Humatae Tyermanni and Radix et Rhizoma Rhei used by 3,4,5 instar larvaes is raised in mixing Ratio is (10%~50%):(10%~50%):(10%~50%), preferred mass ratio are (20%~40%): (20%~40%):(20%~40%), preferred mass ratio are equal proportion, and its ratio sum is equal to 100%.
In some embodiments, Radix Dauci Sativae used by last instar larvae, Jerusalem artichoke, Radix et Rhizoma Rhei and Radix Salviae Miltiorrhizae are raised in mixing Ratio is (10%~50%):(10%~50%):(10%~50%):(10%~50%), preferred mass ratio is (20%~40%):(20%~40%):(20%~40%):(20%~40%), preferred mass ratio are equal proportion, Its ratio sum is equal to 100%.
In some embodiments, the invention provides a kind of rotation raise host of Cordyceps sinensis larva method, including Following steps:
A () newly hatched larvae 1,2 ages raise:Thickness 3cm, the sterilization upland soil that humidity is 55% are added in frame is raised, Uniformly be put into cleaning again, the Polygonum viviparum L. of equal proportion mixing, motion eccentric and Radix et Rhizoma Rhei after sterilization, then add thickness 3cm, humidity again For 55% sterilization upland soil, ovum is put into, is placed in after covering box in 1,000,000 grades of clean areas and simulates Qinghai-Tibet plateau climate raising;
B () 3,4,5 instar larvae is raised:Feedstuff is replaced by into Radix Salviae Miltiorrhizae, Rhizoma Humatae Tyermanni and the Radix et Rhizoma Rhei of equal proportion mixing;
The larva raising of (c) into last age:By feedstuff be replaced by equal proportion mixing Radix Dauci Sativae, Jerusalem artichoke, Radix et Rhizoma Rhei and Radix Salviae Miltiorrhizae.
In some embodiments, feedstuff was once added every 60 days, and was 55% with thickness 3-6cm, humidity Sterilization upland soil is covered.
Term " last age ", in some embodiments, Hepialus armorieanus Oberthur instar larvae totally 7 ages, then last age refer to bat Hepialus larva the 6th, 7 ages;In other embodiments, Hepialus armorieanus Oberthur instar larvae totally 8 ages, then last age refer to Vespertilio Moth larvae the 6th, 7,8 ages.
The equal proportion mentioned in the present invention is equal quality ratio.Term " equal proportion " refers to the content phase of each component Together, or be close to identical (± 5% being differed with identical single component content).For example, in some embodiments, mixing is raised The mass ratio of the Polygonum viviparum L., motion eccentric and Radix et Rhizoma Rhei supported is equal proportion, i.e., the equal proportion can be 1:1.05:1.02, or 1.05:1.02:1.05, or 1:1.03:1.01, etc..
Beneficial effect of the present invention is:
Hobby is taken food for each worm age section of Hepialus armorieanus Oberthur, different foods is fed to different worm instar larvaes, is made host larva whole Individual growth stage can absorb nutrition in a balanced way, solve Larval foods disappearance disease, also ensure that follow-up Cordyceps have excellent product Matter;In addition, each worm age section larva is taking food the plant of oneself liking eating, the larval growth cycle can be accelerated, be extensive feeding Foster Vespertilio moth larvae provides new method and thinking;A kind of plateau feedstuff Radix Salviae Miltiorrhizae is filtered out particularly, reported first is used for Vespertilio The raising of moth larvae, enriches the selection of Hepialus armorieanus Oberthur host-feeding, reduces feed cost.
Specific embodiment
Described below is the preferred embodiment of the present invention, and what the present invention was protected is not limited to the following side of being preferable to carry out Formula.It should be pointed out that for a person skilled in the art on the basis of this innovation and creation design, some deformations for making and Improve, belong to protection scope of the present invention.In embodiment, raw material used can be obtained by commercial sources.
The present invention is that character is stable after the raising of Fromlingzhi, tibet plateau Xue Dou Cordyceps company limited is tamed for test worm kind Vespertilio moth larvae.
Under normal circumstances, insecticide children phase growth will slough off a skin to a certain extent, so its size or growth are entered Journey (i.e. so-called worm age or instar larvae) can make index with number of times is casted off a skin.Just come out to from egg hatching before casting off a skin for the first time Larva be referred to as the 1st instar larvae, Jing first time cast off a skin after larva be referred to as the 2nd instar larvae.Equally, 3 age, 4 ages, 5 ages, 6 ages, 7 Age, larva is respectively second and third, cast off a skin for four, five, six times after larva.
Feeding choice of the different worm age section Vespertilio moth larvaes to different plants, from Qinghai Tibet plateau cordyceps producing region and west Hide the Polygonum viviparum L. of the plant culture production base excavation Hepialus armorieanus Oberthur eating of Linzhi plateau Xue Dou Cordyceps company limited, Radix Salviae Miltiorrhizae, Radix et Rhizoma Rhei, motion eccentric, Dobinea delavayi, Jerusalem artichoke, Rhizoma Humatae Tyermanni, Radix Dauci Sativae remove impurity, fibrous root etc., and clear water is cleaned, and takes back laboratory standby With.
Reference implementation example 1. simulates temperature required Qinghai-Tibet larval growth, precipitation, percentage of possible sunshine
This research simulates used historical climate data from China Meteorological Administration of China Meteorological Administration meteorological data Room is newest《Chinese terrestrial climate data moon Value Data collection》, the data set includes the moon Value Data of national 752 meteorological sites, And quality control is carried out by China Meteorological Administration.Qinghai-Tibet Platean is arranged from the data set and about 205, periphery is meteorological The station was from the moon value meteorological data of 1981 to 2010, and chose the mode input meteorologic parameter such as precipitation, temperature, percentage of possible sunshine Monthly Value Data, sets up meteorogical phenomena database after arrangement.
This research simulation Qinghai-Tibet Platean carries out larva raising, and larval growth is temperature required, precipitation, percentage of possible sunshine parameter setting take Tibetan Plateau Meteorology data base this month meansigma methodss, monthly change a clean area temperature, humidity and percentage of possible sunshine ginseng according to data base Number, wherein the plateau temperature difference is adjusted according to changes in temperature season daily, i.e. cold season (October~March), and day and night temperature sets 2 DEG C, warm season (April~September), day and night temperature set 5 DEG C.
2. Radix Salviae Miltiorrhizae of reference implementation example is screened
Screening process:More than 100 kinds of Chinese herbal medicine or food materials are tested, from taking food effect, shelf-life, larval growth parameter etc. Pick out the following 8 kinds food materials that disclosure satisfy that large-scale production.Radix Salviae Miltiorrhizae and Polygonum viviparum L., Radix et Rhizoma Rhei, motion eccentric, Dobinea delavayi, chrysanthemum Taro, Rhizoma Humatae Tyermanni, Radix Dauci Sativae compare feeding effect such as following table.
Feedstuff Polygonum viviparum L. Radix Dauci Sativae Motion eccentric Radix Salviae Miltiorrhizae Jerusalem artichoke Rhizoma Humatae Tyermanni Radix et Rhizoma Rhei Dobinea delavayi
Larva annual survival rate % 53.46 50.75 47.13 45.39 45.10 43.55 43.87 40.26
1 different selections of the worm age section Vespertilio moth larvae to feedstuff of embodiment
The filter paper for being used for moisturizing is placed in the transparent plastic culture dish of a diameter 12cm, optional food materials rhizome It is cut into 1cm3Square block size, and uniformly directly one circle of placement is on filter paper, every kind of in each device along culture dish surrounding Plant roots and stems are 2 pieces, and such as in this programme, larva carries out feeding choice to 8 kinds of plant roots and stems, and its placement order is ABCDEFGHABCDEFGH.When experiment starts, supplying Jing after Nature enemy 24h is tried 1,2,3,4,5,6,7 instar larvae of Hepialus armorieanus Oberthur The central authorities of 7 moistening filter paper are respectively placed in, and cover culture dish lid respectively, be subsequently placed in 12 DEG C of temperature, humidity 80%, illumination Intensity 15W, L:D=14h:Selectivity is determined under conditions of 10h (light application time within a day and dark processing time ratio), often Individual culture dish places 30 larvas, arranges 5 repetitions.After 6,12,24,48h, on each plant roots and stems of observed and recorded and its around The borer population that falls in the range of 1cm, four observing time node the percentage rate of selection sum reflect worm age section larva to food Preference.
1 different selection percentage rate of the worm age section Vespertilio moth larvae to feedstuff of table
Note:In table, data are mean+SD.With in 0.05 level of different lowercase letters after group data Significant difference.
As seen from the above table, 1,2 instar larvaes to Polygonum viviparum L., motion eccentric and Radix et Rhizoma Rhei feeding choice rate be higher than other feedstuffs, 3, 4th, 5 instar larvae preference Radix Salviae Miltiorrhizaes, Rhizoma Humatae Tyermanni and Radix et Rhizoma Rhei, 6,7 instar larvaes start preference Radix Dauci Sativae, Jerusalem artichoke, Radix et Rhizoma Rhei and Radix Salviae Miltiorrhizae.
Host of Cordyceps sinensis larva is raised in 2 rotation of embodiment
The rotation raising group A flow process of present invention design is that 1,2 instar larvaes adopt Polygonum viviparum L., motion eccentric and Radix et Rhizoma Rhei equal proportion Mixing is raised and followed by changes Radix Salviae Miltiorrhizae, Rhizoma Humatae Tyermanni and Radix et Rhizoma Rhei equal proportion mixing raising into 5 ages, and the larva into last age is adopted Raised together with Radix Dauci Sativae, Jerusalem artichoke, Radix et Rhizoma Rhei and Radix Salviae Miltiorrhizae equal proportion;Matched group B, C, D, E, F, G, H are respectively to larva in whole age list Private Polygonum viviparum L., motion eccentric, Radix et Rhizoma Rhei, Radix Salviae Miltiorrhizae, Rhizoma Humatae Tyermanni, Radix Dauci Sativae, Jerusalem artichoke are raised;Matched group I, J, K are respectively to larva whole Individual age is raised with the mixing of Polygonum viviparum L., motion eccentric and Radix et Rhizoma Rhei equal proportion, and the mixing of Radix Salviae Miltiorrhizae, Rhizoma Humatae Tyermanni and Radix et Rhizoma Rhei equal proportion is raised Support, the mixing of Radix Dauci Sativae, Jerusalem artichoke, Radix et Rhizoma Rhei and Radix Salviae Miltiorrhizae equal proportion is raised.
40 centimetres of raising frame length used, it is wide 28 centimetres, it is high 27 centimetres;Every piece of plant roots and stems cut into 1cm3Cube block Shape, it is standby.
The feedstuff injected volume in different worm ages:1st, 2 instar larvaes are small, food-intake is few, 200, ovum are thrown in per frame, every 60 Its addition corresponding feed 100g;3rd, 4,5 instar larvae food-intakes are big, add corresponding feed 200g per 60 days;6th, 7 instar larvaes start into Enter prepupal period, add corresponding feed 100g every 60 days.
The foster worm treatment group A flow process of rotation is specific as follows,
A () newly hatched larvae 1,2 ages raise:Thickness 3cm, the sterilization upland soil that humidity is 55% are added in frame is raised, Cleaning, the Polygonum viviparum L. of equal proportion mixing after sterilization, motion eccentric and the common 100g of Radix et Rhizoma Rhei are uniformly put into, then add thickness again 3cm, humidity are 55% sterilization upland soil, 200 ovum are thrown in per frame, is placed in 1,000,000 grades of clean areas and raises after being covered box, clean Net area's environmental simulation Tibetan Plateau Meteorology data base this month meansigma methodss, environmental Kuznets Curves are -2 DEG C -14 DEG C of temperature, humidity 30%- 90%;Every 60 days addition correspondence stages mixed fodder 100g, and covered with the sterilization upland soil that thickness 3cm, humidity are 55%;
B () 3,4,5 instar larvae is raised:Feedstuff is changed into Radix Salviae Miltiorrhizae, Rhizoma Humatae Tyermanni and the common 200g of Radix et Rhizoma Rhei of equal proportion mixing; Every 60 days addition correspondence stages mixed fodder 200g, and covered with the sterilization upland soil that thickness 3cm, humidity are 55%;
The larva raising of (c) into last age:By feedstuff be replaced by equal proportion mixing Radix Dauci Sativae, Jerusalem artichoke, Radix et Rhizoma Rhei and The common 100g of Radix Salviae Miltiorrhizae;Every 60 days addition correspondence stages mixed fodder 100g, and covered with the sterilization upland soil that 3cm humidity is 55% Lid.
11 process feedstuff groups (rotation processes raising group and 10 matched groups) flow process feeding will be raised according to respective above Feed with a collection of newly hatched larvae, each processes 5 repetitions, according to the larva in different worm ages, is raised using DIFFERENT FEED, feedstuff Once added every 60 days, and covered with the sterilization upland soil that thickness 3cm, humidity are 55%, each is processed gained pupa list Solely weigh, and carry out emergence mating, calculate female adult eggs on average rate and egg laying amount, by each process egg hatching, continue live box and obtain Larva.
The raising that three larvas by a definite date go through the phase, the maintenance data such as following table of Hepialus armorieanus Oberthur are carried out:
As seen from table, in terms of survival rate of larvae:Rotation treatment group is significantly higher than other ten matched groups;Average pupa heavy prescription Face:Four treatment groups do not have a notable difference, spawning rate, eggs on average amount and complete three larvas and go through phase permitted average time side Face:Rotation raising group is better than ten matched groups;From result, it is adapted to Vespertilio using the rotation feeding strategies that the present invention is provided Moth larvae grows, and the short period can just complete the circulation of one growth cycle of Hepialus armorieanus Oberthur, be conducive to Cordyceps industrialization to carry out.

Claims (10)

1. a kind of method that host of Cordyceps sinensis larva is raised in rotation, comprises the following steps:
(1) 1,2 instar larvaes are raised with the mixing of Polygonum viviparum L., motion eccentric and Radix et Rhizoma Rhei, wherein mix the mass ratio of raising for etc. Ratio;
(2) continue the mixing of 3,4,5 instar larvae Radix Salviae Miltiorrhizaes, Rhizoma Humatae Tyermanni and Radix et Rhizoma Rhei to raise, wherein mixing the mass ratio of raising For equal proportion;
(3) larva into last age is raised with the mixing of Radix Dauci Sativae, Jerusalem artichoke, Radix et Rhizoma Rhei and Radix Salviae Miltiorrhizae, wherein mixing the quality of raising Ratio is equal proportion.
2. method according to claim 1, wherein, methods described is raised using the raising frame equipped with sterilization upland soil Support, and upland soil THICKNESS CONTROL of sterilizing is:Feedstuff is gone to the bottom floor height original soil thickness at least 3cm, feedstuff upper epidermis plateau soil thickness 3- 6cm。
3. method according to claim 2, wherein, the humid control of upland soil of sterilizing is in 45%-65%.
4. method according to claim 1, wherein, between the raising that methods described is adopted, purity requirements are 1,000,000 grades.
5. method according to claim 1, the weather conditions of larva raising are simulation Qinghai-Tibet plateau climate.
6. method according to claim 1, wherein, the temperature controlling range of larva raising is -2 DEG C -14 DEG C.
7. method according to claim 1, wherein, the humid control scope of larva raising is 30%-90%.
8. method according to claim 1, wherein, the temperature controlling range of larva raising is -2 DEG C -14 DEG C, humid control Scope is 30%-90%.
9. method according to claim 1, comprises the following steps:
A () newly hatched larvae 1,2 ages raise:Addition thickness 3cm, the sterilization upland soil that humidity is 55% in frame is raised, then It is even be put into cleaning, the Polygonum viviparum L. of equal proportion mixing, motion eccentric and Radix et Rhizoma Rhei after sterilization, then adding thickness 3cm, humidity again is 55% sterilization upland soil, ovum is put into, and is placed in 1,000,000 grades of clean areas and simulates Qinghai-Tibet plateau climate raising after covering box;
B () 3,4,5 instar larvae is raised:Feedstuff is replaced by into Radix Salviae Miltiorrhizae, Rhizoma Humatae Tyermanni and the Radix et Rhizoma Rhei of equal proportion mixing;
The larva raising of (c) into last age:Feedstuff is replaced by into Radix Dauci Sativae, Jerusalem artichoke, Radix et Rhizoma Rhei and the pellet of equal proportion mixing Ginseng.
10. method according to claim 9, wherein, feedstuff was once added every 60 days, and with thickness 3-6cm, wet Spend the sterilization upland soil covering for 55%.
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CN107198060B (en) * 2017-06-23 2020-12-22 东莞市东阳光冬虫夏草研发有限公司 Cordyceps sinensis host larva feed and preparation method thereof
CN108184773B (en) * 2018-03-15 2020-03-31 安发(福建)生物科技有限公司 Method for improving survival rate of low-altitude bred bat moth larvae
CN112314535B (en) * 2020-11-27 2022-08-12 东莞市东阳光冬虫夏草研发有限公司 Cordyceps sinensis host larva feed and method for feeding cordyceps sinensis host larvae
CN112586454A (en) * 2020-12-15 2021-04-02 青海珠峰冬虫夏草工程技术研究有限公司 Large-scale breeding method for cordyceps sinensis host hepialus armoricanus larvae

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