CN112314535B - Cordyceps sinensis host larva feed and method for feeding cordyceps sinensis host larvae - Google Patents
Cordyceps sinensis host larva feed and method for feeding cordyceps sinensis host larvae Download PDFInfo
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- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 125
- 238000000034 method Methods 0.000 title claims description 54
- 241001289529 Fallopia multiflora Species 0.000 claims abstract description 15
- 239000002689 soil Substances 0.000 claims description 31
- 240000000275 Persicaria hydropiper Species 0.000 claims description 11
- 235000017337 Persicaria hydropiper Nutrition 0.000 claims description 11
- 229910052785 arsenic Inorganic materials 0.000 claims description 10
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 claims description 10
- 241000330896 Thitarodes armoricanus Species 0.000 claims description 7
- 235000008081 Rheum officinale Nutrition 0.000 claims description 6
- 240000004980 Rheum officinale Species 0.000 claims description 6
- 230000000384 rearing effect Effects 0.000 claims description 5
- 230000003749 cleanliness Effects 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 description 16
- 241000205407 Polygonum Species 0.000 description 16
- 239000000463 material Substances 0.000 description 10
- 241000190633 Cordyceps Species 0.000 description 8
- 244000057214 Stachys sieboldii Species 0.000 description 6
- 235000005116 Stachys sieboldii Nutrition 0.000 description 6
- 235000021050 feed intake Nutrition 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 229910001385 heavy metal Inorganic materials 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 241000123968 Paeonia anomala Species 0.000 description 4
- 239000004459 forage Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000758993 Equisetidae Species 0.000 description 3
- 235000018167 Reynoutria japonica Nutrition 0.000 description 3
- 240000001341 Reynoutria japonica Species 0.000 description 3
- 241000219061 Rheum Species 0.000 description 3
- 244000216000 Rheum emodi Species 0.000 description 3
- 235000014170 Rheum emodi Nutrition 0.000 description 3
- 235000009411 Rheum rhabarbarum Nutrition 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241001517299 Bulbophyllum Species 0.000 description 2
- 206010062717 Increased upper airway secretion Diseases 0.000 description 2
- 206010040844 Skin exfoliation Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000035618 desquamation Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 235000012631 food intake Nutrition 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 235000008216 herbs Nutrition 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 208000026435 phlegm Diseases 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 208000010228 Erectile Dysfunction Diseases 0.000 description 1
- 241000130660 Hepialidae Species 0.000 description 1
- 241000330899 Hepialus Species 0.000 description 1
- 244000058108 Persicaria minor Species 0.000 description 1
- 206010041497 Spermatorrhoea Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
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- 238000007726 management method Methods 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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Abstract
The invention provides a feed for host larvae of cordyceps sinensis. The feed comprises polygonum multiflorum. The feed can improve the hay weight of the cordyceps sinensis host larvae and reduce the content of As. Meanwhile, the feed is easy to obtain, can relieve the shortage of the cordyceps sinensis host plateau feed, and has wide application prospect.
Description
Technical Field
The invention relates to the field of biology, in particular to a cordyceps sinensis host larva feed and a method for feeding cordyceps sinensis host larvae.
Background
Cordyceps sinensis, name of Chinese traditional medicine. Is a complex of the stroma of Cordyceps sinensis (BerK.) Sacc, a plant of the family Clavicipitaceae, parasitic on larvae of insects of the family Hepialidae, and the cadaver of the larvae. Has effects of invigorating kidney, benefiting lung, stopping bleeding, and eliminating phlegm. Can be used for treating sexual impotence, spermatorrhea, soreness of waist and knees, cough, asthma, and phlegm blood.
According to the limit standards of heavy metals and harmful elements of botanical drugs in the current 2015 version of Chinese pharmacopoeia, arsenic (As) is the main harmful element pollutant in the cordyceps sinensis medicinal materials. In recent years, the over standard arsenic content of the cordyceps sinensis is a key point to be questioned, and the development of the cordyceps sinensis industry is influenced.
At present, most of the related technologies relate to the treatment of removing heavy metal and harmful element pollutants from cordyceps sinensis finished products, although the heavy metal content can be reduced, the process is complex, and the nutrition damage and the cost increase caused by cordyceps sinensis are immeasurable. Therefore, the effective way for solving the problem of heavy metal of the cordyceps sinensis is to scientifically cultivate the cordyceps sinensis and prevent the cordyceps sinensis in advance.
At present, the content of As in cordyceps sinensis and soil in a production area is seriously exceeded, and the serious pollution level is reached, so that the As pollution in cordyceps sinensis medicinal materials is inseparable from the soil. However, the whole life cycle of wild cordyceps sinensis needs 3-5 years, most of the time is in the larva growth period, and in the long period, except soil, the feed taken by the larva is one of the important factors related to cordyceps sinensis As. And As is mostly concentrated in the worm body, the stroma is less, and the side surface also verifies the point.
Therefore, the feed for the cordyceps sinensis host larvae and the method for feeding the cordyceps sinensis host larvae need to be improved.
Disclosure of Invention
The present invention aims to solve at least to some extent at least one of the technical problems of the prior art. Therefore, the invention provides a cordyceps sinensis host larva feed, a method for feeding cordyceps sinensis host larvae, a method for increasing the weight of hay of the cordyceps sinensis host larvae and a method for reducing arsenic content in cordyceps sinensis. The feed can improve the hay weight of the cordyceps sinensis host larvae and reduce the content of As. Meanwhile, the feed is easy to obtain, can relieve the shortage of the cordyceps sinensis host plateau feed, and has wide application prospect.
In one aspect of the invention, the invention provides a cordyceps sinensis host larva feed. According to an embodiment of the invention, the feed comprises: fleece-flower root. The fodder can be used for raising host larva of Cordyceps, and has effects of increasing hay weight of host larva of Cordyceps, and reducing As content. Meanwhile, the feed is easy to obtain, can relieve the shortage of the cordyceps sinensis host plateau feed, and has wide application prospect.
According to an embodiment of the invention, the feed may also have the following additional technical features:
according to an embodiment of the invention, the feed further comprises: polygonum bulbiferum, Rheum emodi, Polygonum hydropiper and/or Paeonia anomala.
According to an embodiment of the present invention, the host larvae of cordyceps sinensis are hepialus armoricanus larvae.
In another aspect of the present invention, the present invention provides a method for rearing host larvae of Cordyceps sinensis. According to an embodiment of the invention, the method comprises: feeding the cordyceps sinensis host larvae with the feed. The method for breeding the cordyceps sinensis host larvae improves the weight of the cordyceps sinensis host larvae hay, and meanwhile, the cultivated cordyceps sinensis has low content of As.
According to an embodiment of the invention, the method comprises: and alternately feeding different feeds for the cordyceps sinensis host larvae based on different growth periods of the cordyceps sinensis host larvae.
According to an embodiment of the invention, the method comprises: feeding rheum officinale to host larvae of 1-year and 2-year cordyceps sinensis; feeding polygonum hydropiper linn to host larvae of 3-year-old cordyceps sinensis; feeding 4-instar Cordyceps sinensis host larvae with radix Paeoniae; feeding 5-year and 6-year aweto host larvae with polygonum multiflorum; feeding 7-year-old host larvae of Cordyceps sinensis with Polygonum hydropiper.
According to an embodiment of the invention, the method comprises: and (3) feeding the cordyceps sinensis host larvae into a soil layer containing the feed.
According to the embodiment of the invention, the mass ratio of the feed to the soil in the soil layer is (0.5-1.2): 1, the soil is sterile plateau soil, the humidity is 25-55%, and the thickness of the soil layer is 10-30 cm.
According to the embodiment of the invention, the cordyceps sinensis host larvae are bred under the conditions that the temperature is-10-25 ℃, the humidity is 30-99% and the cleanliness is 100 ten thousand.
According to an embodiment of the present invention, the cordyceps sinensis host larvae are hepialus armoricanus larvae.
In another aspect of the present invention, the present invention provides a method for increasing the weight of cordyceps sinensis hay. According to an embodiment of the invention, the method comprises: the feed is fed to the host larvae of the cordyceps sinensis, or the host larvae of the cordyceps sinensis are fed by adopting the method. As described above, the forage for feeding the host larvae of the cordyceps sinensis provided by the embodiment of the invention can increase the hay weight of the host larvae of the cordyceps sinensis and reduce the As content.
In another aspect of the present invention, the present invention provides a method for reducing arsenic content in Cordyceps sinensis. According to an embodiment of the present invention, the method comprises feeding the above-mentioned feed to the host larvae of Cordyceps sinensis, or feeding the host larvae of Cordyceps sinensis by the above-mentioned method. As described above, the forage for feeding the host larvae of the cordyceps sinensis provided by the embodiment of the invention can increase the hay weight of the host larvae of the cordyceps sinensis and reduce the As content.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
The following describes embodiments of the present invention in detail. The following examples are illustrative only and are not to be construed as limiting the invention.
The invention provides a feed for cordyceps sinensis host larvae, a method for feeding the cordyceps sinensis host larvae, a method for increasing the weight of hay in the cordyceps sinensis host larvae and a method for reducing arsenic content in cordyceps sinensis, which are respectively described in detail below.
Cordyceps sinensis host larva feed
In one aspect of the invention, the invention provides a cordyceps sinensis host larva feed. According to an embodiment of the invention, the feed comprises: fleece-flower root. The inventor tests nearly hundreds of Chinese herbal medicines or food materials, screens plateau food material fleece-flower root from the aspects of food taking effect, quality guarantee period, larva growth parameters and the like, and feeds the cordyceps sinensis host larva by adopting the feed, so that the hay weight of the cordyceps sinensis host larva can be increased, and the As content can be reduced. Meanwhile, the feed is easy to obtain, can relieve the shortage of the cordyceps sinensis host plateau feed, and has wide application prospect.
According to an embodiment of the invention, the feed further comprises: polygonum bulbiferum, Rheum emodi, Polygonum hydropiper and/or Paeonia anomala. The inventor discovers through screening experiments that the addition of polygonum bulbiferum, rhubarb horsetails, polygonum rotundus and radix paeoniae rubra in the feed can well increase the hay weight of host larvae of cordyceps sinensis and reduce the As content.
According to an embodiment of the present invention, the host larvae of Cordyceps sinensis are hepialus armoricanus larvae.
Method for breeding host larvae of Cordyceps sinensis
In another aspect of the present invention, the present invention provides a method for rearing host larvae of Cordyceps sinensis. According to an embodiment of the invention, the method comprises: feeding the cordyceps sinensis host larvae with the feed. As mentioned above, the feed can improve the feed intake of the host larvae of the cordyceps sinensis, and the content of the cultured cordyceps sinensis As is low.
According to an embodiment of the invention, the method comprises: based on different growth periods of the cordyceps sinensis host larvae, the cordyceps sinensis host larvae are fed with different feeds alternately. The inventor finds that in the feeding process, compared with the feeding of a single feed, the alternate feeding of the feed can better improve the weight of cordyceps sinensis host larva hay, and the content of cultivated cordyceps sinensis As is low.
According to an embodiment of the invention, the method comprises: feeding rheum officinale to host larvae of 1-year and 2-year cordyceps sinensis; feeding polygonum hydropiper linn to host larvae of 3-year-old cordyceps sinensis; feeding 4-instar Cordyceps sinensis host larvae with radix Paeoniae; feeding 5-year and 6-year aweto host larvae with polygonum multiflorum; feeding 7-year-old host larvae of Cordyceps sinensis with Polygonum hydropiper. Based on the fact that the feed has different As enrichment, absorption and transfer capacities in the wild and the combination of the feed intake of larvae in respective instar stages, the inventor finds that the weight of cordyceps sinensis host larva hay can be better improved in the alternate feeding scheme, and the content of cultivated cordyceps sinensis As is low.
According to an embodiment of the invention, the method comprises: feeding Cordyceps host larva into soil layer containing feed. The Cordyceps sinensis host larvae are fed into soil and feed, which is helpful for larva growth, and can increase hay weight and reduce As content.
According to the embodiment of the invention, the mass ratio of the feed to the soil in the soil layer is (0.5-1.2): 1, the soil is sterile plateau soil, the humidity is 25-55%, and the thickness of the soil layer is 10-30 cm. The inventor finds that the feed and the soil with the mass ratio of (0.5-1.2): 1 can optimize the space distribution structure of the soil and the natural plant roots and stems to the maximum extent, so that larvae can live and eat in the mixed layer conveniently, the mixed layer with the thickness of 10-30 cm can provide the larvae with a loose physical structure state with good air permeability, can also effectively control the excrement of the larvae and maintain the microenvironment required by the larvae, and the sterile plateau soil humidity of 25-55% can provide a growth microenvironment suitable for the bat moth larvae.
According to the embodiment of the invention, the cordyceps sinensis host larvae are bred under the conditions that the temperature is-10-25 ℃, the humidity is 30-99% and the cleanliness is 100 ten thousand. The inventor finds that the feeding conditions simulate the climate environment required by larva growth, and are more suitable for the growth of cordyceps sinensis host larvae.
It should be noted that the features and advantages described above for the feed for the host larvae of Cordyceps sinensis are also applicable to the method for feeding the host larvae of Cordyceps sinensis.
Method for increasing weight of cordyceps sinensis hay
In another aspect of the present invention, the present invention provides a method for increasing the weight of cordyceps sinensis hay. According to an embodiment of the invention, the method comprises feeding the host larvae of the cordyceps sinensis, or feeding the host larvae of the cordyceps sinensis by using a feeding method. As described above, the forage for feeding the host larvae of the cordyceps sinensis provided by the embodiment of the invention can increase the hay weight of the host larvae of the cordyceps sinensis and reduce the As content.
It should be noted that the features and advantages described above for the cordyceps sinensis host larva fodder and the method for raising cordyceps sinensis host larva are also applicable to the method for increasing cordyceps sinensis hay weight, and are not described herein again.
Method for reducing arsenic content in cordyceps sinensis
In another aspect of the present invention, the present invention provides a method for reducing arsenic content in Cordyceps sinensis. According to an embodiment of the invention, the method comprises feeding the host larvae of cordyceps sinensis, or feeding the host larvae of cordyceps sinensis by using a feeding method. As described above, the forage for feeding the host larvae of the cordyceps sinensis provided by the embodiment of the invention can increase the hay weight of the host larvae of the cordyceps sinensis and reduce the As content.
It should be noted that normally, an insect larva will be skinned once after it has grown to a certain extent, so that its size or progress of growth (so-called age or age of the larva) can be indicated by the number of skinnings. Larvae just hatched before the first molt are called larvae at age 1, and larvae after the first molt are called larvae at age 2. Similarly, the larvae of 3 rd, 4 th, 5 th, 6 th and 7 th are those after the second, third, fourth, fifth and sixth molting respectively.
It should be noted that the features and advantages described above for the cordyceps sinensis host larva feed and the method for feeding cordyceps sinensis host larvae are also applicable to the method for reducing arsenic content in cordyceps sinensis, and are not described herein again.
The scheme of the invention will be explained with reference to the examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples, where specific techniques or conditions are not indicated, are to be construed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are conventional products which are commercially available, and are not indicated by manufacturers.
Example 1 study of the Effect of different food materials or medicinal materials on the annual survival Rate of larvae
The inventor takes the feeding effect, the quality guarantee period, the larva growth parameters and the like of the larva as indexes to investigate more than one hundred kinds of Chinese herbal medicines or food materials. Table 1 shows how many herbs with relatively high annual survival rate of larvae are Polygonum multiflorum, Polygonum bulborum, Polygonum rotundus, Paeonia anomala, Stachys sieboldii and Rheum emodi. It can be seen that the annual survival rate of the larvae eating polygonum multiflorum is higher than that of other 5 medicinal materials.
TABLE 1 Effect of different herbs on the annual survival of larvae
| Feed stuff | Polygonum bulbophyllum | Polygonum multiflorum Thunb | Polygonum hydropiper (L.) Druce | Radix Paeoniae | Stachys sieboldii (Hemsl.) Merr | Radix et rhizoma Rhei |
| Annual survival rate of larvae% | 53.46 | 49.13 | 47.13 | 47.10 | 43.55 | 43.87 |
Example 2 rootstock As content of plants
In a plant cultivation production base of Tibet Linzhi plateau Xuediu Cordyceps, class III soil (soil quality basically does not cause harm and pollution to plants and environment) meeting the soil environment quality standard (GB15618-1995) is selected, a plurality of cultivation greenhouses are established, Polygonum bulbiferum, Polygonum multiflorum, rheum officinale, Polygonum rotundus, Paeonia anomala and Stachys sieboldii are respectively planted in a sowing season, 5 parts of the whole plant of the plant are collected after rhizomes are mature (used for parallel sample determination), overground parts and fibrous roots are removed, and the plant is cleaned by clear water and taken back to a laboratory for later use.
Referring to the general rule of the Chinese pharmacopoeia 2015 edition (four parts), the variation of heavy metal As of various plant roots is measured by inductively coupled plasma mass spectrometry, and the content of rhizome As is shown in Table 2.
TABLE 2 rootstock As content (unit mg/kg) of different plants
| Feed stuff | Polygonum bulbophyllum | Polygonum multiflorum Thunb | Polygonum hydropiper (L.) Druce | Radix Paeoniae | Stachys sieboldii (Hemsl.) Merr | Radix et rhizoma Rhei |
| Content of As | 0.59 | 0.48 | 0.61 | 0.66 | 0.73 | 0.79 |
As can be seen from Table 2, under the same soil conditions, the enrichment, absorption and transport capacities of different plant rootstock As are different, wherein the content of Polygonum multiflorum As is the lowest.
Example 3 diet measurement and alternate feeding scheme design for 6 plants at different ages
The test insect species are hepialus armoricanus larvae, 100 larvae are treated and raised in each instar group, the larvae are raised in a culture dish in a single head, the culture dish is placed in a constant-temperature incubator at 12 ℃, polygonum bulboruifolium, polygonum multiflorum, rheum officinale, polygonum rotundus, radix paeoniae, and stachys sieboldii are respectively raised, insect feces are cleaned and feeds are replaced according to conditions, and the larva desquamation date and desquamation time are observed and recorded. When the feed is replaced, A, B parts of feed with the same quality and the same quantity are weighed each time, wherein A part is used for feeding larvae, and B part is baked in an oven at 55 ℃ until the weight is constant for later use. And when the feed is replaced next time, collecting the part which is not eaten completely in the part A and processing the part A according to the method of the part B. The total feed intake (dry matter) of the insect in the same age is obtained by subtracting the constant weight of the rest feed in the A from the constant weight of the B feed in the same age. Namely, the food intake (dry matter mass) of the larva at a certain age is calculated according to the following formula:
the feed intake of the larva of a certain age is equal to the total constant weight (g) of B parts of feed in the whole period of the certain age, and the total constant weight (g) of the rest part of A parts of feed in the certain age.
The feed intake data of the hepialus larva at each age are shown in table 3.
TABLE 3 Bat moth larval instar food intake (g)
The accumulated amount of As of the rootstock of different plants eaten by the larvae of a certain age is the content of As of the rootstock of the corresponding plant eaten by the larvae of a certain age.
The accumulation of As in the different plant rootstocks from Hepialus armoricanus is shown in Table 4.
TABLE 4 accumulation of As from different plant rootstocks taken by hepialus armoricanus (ug)
As can be seen from tables 3 and 4, the larvae of 1 and 2 years old are bred by rhubarb horsetails, the larvae of 3 years old are bred by polygonum minus, the larvae of 4 years old are bred by radix paeoniae rubra, the larvae of 5 and 6 years old are bred by polygonum multiflorum, and the larvae of 7 years old are bred by polygonum bulbosum, so that the total feed intake amount of the whole larvae in the instar stage is the most, but the theoretical accumulation amount of As is 3.7147, and is less than that of other known plateau feeds except polygonum multiflorum.
Example 4 alternate feeding of Cordyceps sinensis host larvae
In the embodiment, the cordyceps sinensis host larvae are fed in an alternate feeding mode, the specific alternate feeding process is that larvae of 1 and 2 ages are fed with rhubarb horsetails, larvae of 3 ages are fed with polygonum hydropiper, larvae of 4 ages are fed with radix paeoniae, larvae of 5 and 6 ages are fed with polygonum multiflorum respectively, and larvae of 7 ages are fed with polygonum bulbosum. Meanwhile, a control group is set, wherein the control groups 1, 2, 3, 4, 5 and 6 are respectively used for feeding larvae with polygonum bulbiferum, new feed polygonum multiflorum, polygonum rotundus, radix paeoniae, stachys sieboldii and rheum officinale separately in the whole instar.
The used breeding frame is 40 cm long, 28 cm wide and 27 cm high.
50 eggs are put in each frame, and 100g of feed and sterile plateau soil are replaced at each age.
Feeding the seven treated feed groups (alternate feeding group and control group 1-6) to the same batch of primarily hatched larvae according to respective feeding processes, and repeating each treatment for 5 times.
The test strains are provided by Xizang Linzhi plateau Xuediu Cordyceps, and refer to "Cordyceps cultivation technical Specification" (DB 42/T1272-2017) to perform Cordyceps inoculation, management and harvest after inoculation, and finally perform hay weight determination and corresponding As content determination on each treated Cordyceps, As shown in Table 5 below.
TABLE 5 hay weight and As content
As can be seen from Table 5, the As content of the finished Cordyceps sinensis products obtained from the alternate rearing group and the control groups 1-6 both meet the Chinese pharmacopoeia standard (less than or equal to 2ppm), but the alternate rearing group has the fastest growth and development because the total amount of the feed ingested by the whole larva at the age stage is the largest, so that the life time of the cordyceps sinensis products in microenvironment such As excrement and soil is the shortest, and the As content is obviously lower than that of the control groups 1-6. Research reports that most As of wild cordyceps sinensis exceeds the standard. The total amount of the feed ingested by the whole larva in the instar period of the alternate feeding group is the largest, so that the corresponding hay has the heaviest weight, the commercial value of the hay is greatly improved, and the alternate feeding scheme provided by the invention is comprehensively considered and is more suitable for cultivating high-quality cordyceps sinensis with low As.
In the description of the specification, reference to the description of "one embodiment," "some embodiments," "an example," "a specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (4)
1. A method for rearing host larvae of Cordyceps sinensis to reduce arsenic content in Cordyceps sinensis, said method comprising:
feeding rheum officinale to host larvae of 1-year and 2-year cordyceps sinensis;
feeding polygonum hydropiper linn to host larvae of 3-year-old cordyceps sinensis;
feeding 4-instar Cordyceps sinensis host larvae with radix Paeoniae;
feeding 5-year and 6-year aweto host larvae with polygonum multiflorum;
feeding 7-year-old host larvae of Cordyceps sinensis with Polygonum hydropiper.
2. The method according to claim 1, characterized in that it comprises:
feeding the cordyceps sinensis host larvae into a soil layer containing a feed, wherein the feed comprises: polygoni Multiflori radix, herba Polygoni Avicularis, radix et rhizoma Rhei Palmati, herba Polygoni Avicularis and radix Paeoniae;
the mass ratio of the feed to the soil in the soil layer is 0.5-1.2: 1, the soil is sterile plateau soil, the humidity is 25-55%, and the thickness of the soil layer is 10-30 cm;
the cordyceps sinensis host larvae are bred under the conditions that the temperature is-10-25 ℃, the humidity is 30-99% and the cleanliness is 100 ten thousand.
3. The method of claim 1, wherein said host larvae of Cordyceps sinensis are larvae of hepialus armoricanus.
4. A method for increasing the weight of cordyceps sinensis hay, which is characterized by comprising the following steps:
the method for feeding host larvae of Cordyceps sinensis as claimed in any one of claims 1 to 3 to reduce arsenic content in Cordyceps sinensis.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103110020A (en) * | 2013-03-08 | 2013-05-22 | 吉林省农业科学院 | Asiatic corn borer artificial feed containing Chinese herbal medicine additives and preparation method of artificial feed |
| CN106070077A (en) * | 2016-07-02 | 2016-11-09 | 广东东阳光药业有限公司 | The method of host of Cordyceps sinensis larva is raised in a kind of rotation |
| CN106343276A (en) * | 2016-09-23 | 2017-01-25 | 王云 | Feed for feeding cordyceps sinensis host larvae and method for feeding host larvae |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103110020A (en) * | 2013-03-08 | 2013-05-22 | 吉林省农业科学院 | Asiatic corn borer artificial feed containing Chinese herbal medicine additives and preparation method of artificial feed |
| CN106070077A (en) * | 2016-07-02 | 2016-11-09 | 广东东阳光药业有限公司 | The method of host of Cordyceps sinensis larva is raised in a kind of rotation |
| CN106343276A (en) * | 2016-09-23 | 2017-01-25 | 王云 | Feed for feeding cordyceps sinensis host larvae and method for feeding host larvae |
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