CN103110020A - Asiatic corn borer artificial feed containing Chinese herbal medicine additives and preparation method of artificial feed - Google Patents
Asiatic corn borer artificial feed containing Chinese herbal medicine additives and preparation method of artificial feed Download PDFInfo
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Abstract
The invention discloses an Asiatic corn borer artificial feed containing Chinese herbal medicine additives. The Asiatic corn borer artificial feed comprises the following components by weight: 120.0g of soybean flour, 150.0g of corn flour, 60.0g of sucrose, 40.0g-80.0g of beer yeast, 4.0g of ascorbic acid, 10.0g-20.0g of agar, 4.0g of sorbic acid, 0.15g of erythrocin, 0.6ml of formalin, 1000.0ml-1500.0ml of distilled water and 20.0ml-40.0ml of Chinese herbal medicine additives, wherein the Chinese herbal medicine additives are prepared from the following components by weight: 50.0g-150.0g of balloon flowers, 500.0g-700.0g of southernwoods, 50.0g-150.0g of roots of Chinese pulsatilla, 50.0g-150.0g of fleece-flower roots and 50.0g-150.0g of agrimony. The Asiatic corn borer artificial feed disclosed by the invention is reasonable in prescription and comprehensive in nutrient substances, and capable of enhancing the disease-preventing capacity of the Asiatic corn borer, improving the pupal weight and the egg laying amount of the adult borers and avoiding retrogression of the laboratory population.
Description
Technical field
The present invention relates to a kind of insect feedstuff and compound method thereof that contains the Chinese herb feed additive composition, be specifically related to a kind of Ostrinia furnacalis man-made feeds and compound method thereof that contains the Chinese herb feed additive composition.
Background technology
Ostrinia furnacalis Ostrinia furnacalis (Guen é e) is the most important insect of China's corn, is having a strong impact on corn yield and the quality in corn producing region.Efficiently, the exploitation of low toxicity chemical pesticide and novel biopesticide and transgenic insect-resistant corn breeding are three important research directions of comprehensive regulation Ostrinia furnacalis.Efficiently, the screening of low toxicity chemical pesticide and novel biopesticide is identified with mensuration, transgenic corns insect resistace and breed breeding etc. all needs to grow consistent Ostrinia furnacalis test and use worm in a large number, the success or not that the indoors artificial of Ostrinia furnacalis is raised has become that impact is efficient, the low toxicity chemical pesticide, novel biopesticide is developed and the restraining factors of transgenic insect-resistant corn Breeding progress.
It is the new No. 7 man-made feeds (Song Yanying that the Zhou Darong researcher of Plant Protection institute, Chinese Academy of Agricultral Sciences equals exploitation in 1979 that the indoors artificial of Ostrinia furnacalis is raised the most frequently used formula; Zhou Darong; He Kang comes. and " Ostrinia furnacalis is without research and the application of agar meridic diet " " plant protection journal "; 1999,26 (4): 324-328.).This feed formula is in the practice of the mass rearing of Ostrinia furnacalis, and along with raising increasing from generation to generation, degradation phenomena in various degree can appear in the Ostrinia furnacalis population of raising, and outstanding behaviours is that the egg laying amount that pupa heavily reduces with adult reduces.
Comprise sorbic acid, formaldehyde and erythromycin in above-mentioned feed formula, bacterium and the fungi of carrying for feed and polypide have good control action, but ultra-clean insect box microenvironment also can cause going down of corn borer diseases prevention, resistance against diseases, thereby affect survival and the reproduction of Ostrinia furnacalis, cause pupa heavily to reduce with the egg laying amount of adult and reduce.
Therefore, solve population degeneration in the insect raising, at first set about from the insect prevention from suffering from the diseases, comprehensive existing investigative technique by add medicinal herb components in man-made feeds, strengthens the immunity of insect, strengthen the health care of insect, control thereby reach the purpose that population degenerates.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Ostrinia furnacalis man-made feeds that contain the Chinese herb feed additive composition, feed optimizing of the present invention Ostrinia furnacalis feed ingredient proportioning and scientific and reasonable compound method is provided.
The technical solution used in the present invention is: a kind of Ostrinia furnacalis man-made feeds that contain the Chinese herb feed additive composition, the component that comprises following weight: the formalin 1.6ml of soy meal 120.0g, corn flour 150.0g, sucrose 60.0g, brewer's yeast 40.0-80.0g, ascorbic acid 4.0g, agar 10.0-20.0g, sorbic acid 4.0g, erythromycin 0.15g, 35%-42% and distilled water 1000.0-1500.0ml also comprise Chinese herb feed additive 20.0-40.0ml; Described Chinese herb feed additive is aqua, is decocted by the component of following weight to make: balloonflower root 50.0-150.0g, sweet wormwood 500.0-700.0g, Chinese bulbul 50.0-150.0g, fleece-flower root 50.0-150.0g, hairyvein agrimony 50.0-150.0g.
The described compound method that contains the Ostrinia furnacalis man-made feeds of Chinese herb feed additive composition comprises the following steps:
(1) raw material sterilization: soy meal, corn flour, brewer's yeast are put into abundant cooling the preservation at-20 ℃ of refrigerator-freezers afterwards of baking oven sterilization;
(2) preparation A component: the various raw materials that take after step (1) is sterilized mix the component as A;
(3) preparation B component: take agar, with the dissolving of 100.0ml distilled water, join after dissolving in the stainless-steel pan that contains 600.0-1100.0ml distilled water, boil, cooling rear as the B component;
(4) preparation C component: take sucrose, ascorbic acid, erythromycin and sorbic acid, add the distilled water dissolving, as the C component;
(5) the D component is Chinese herb feed additive;
(6) the E component is the formalin of 35%-42%;
(7) component C, D and E are joined in the B component, join after mixing in the A component, fully mix, the room temperature cooled and solidified makes product.
Described in preferred steps (1), oven temperature is 115-125 ℃, and sterilization time is 1-3 hour.
Described in preferred steps (3), distilled water is room temperature.Described chilling temperature is 55-65 ℃.
Distilled water described in preferred steps (4) is 300.0ml, 35-45 ℃.
The preparation of D component described in preferred steps (5) comprises the following steps:
1) balloonflower root, sweet wormwood, the Chinese bulbul, the fleece-flower root and hairyvein agrimony are taken according to proportioning, gross weight is 1000.0g;
2) add distilled water, soak and boil liquid;
3) liquid that obtains of step in filtration is sterilized after naturally cooling at a certain temperature, namely gets Chinese herb feed additive.
Preferred D component preparation steps 2) described in, water is 4000.0-5000.0ml distilled water, and described soak time is 25-35 minute, and described boiling time is 25-35 minute.
Preferred D component preparation steps 3) described in, sterilising temp is 120 ℃, and sterilization time is 20 minutes.
In order to illustrate that feed formula of the present invention can significantly improve Ostrinia furnacalis male and female pupa and heavily reach the indexs such as Adult worms producting eggs amount, new No. 7 feeds that man-made feeds of the present invention and the Zhou Darong researcher of Plant Protection institute, Chinese Academy of Agricultral Sciences equaled exploitation in 1979 have carried out the feeding comparative trial.
Test material and method are as follows:
Confession examination worm source: from the Overwintering Larvae that field, Gongzhuling of Jilin Province city gathers, the Adult worms producting eggs that obtains after termination of diapause is through the population of indoors artificial feed captive breeding;
For the examination feed: adopt the prepared feed of the embodiment of the present invention two and according to the new No. 7 prepared feeds of filling a prescription;
Test method: raise respectively Ostrinia furnacalis with two kinds of feeds under identical raising box and raising condition, each is raised box and connects 40, corn borer pieces of an egg, and every kind of feed raised 3 boxes, is that 3 tests repeat.Whole test was raised for 3 generations continuously.To respectively repeat resulting pupa and differentiate male and female, and get respectively each 20 of female, male pupas and weigh.Then after female, the male pupa that each feed 3 times is repeated gained mixes respectively, therefrom get at random each 150 of female, male pupas, put into altogether 3 cages of laying eggs, each each 50 of cage male and female pupa of laying eggs.Above cage, the lay stencil paper is one, and when laying eggs beginning wait sprouting wings, every day, piece number that record is laid eggs, calculated every average egg laying amount.The feeding effect of last and egg laying amount 3 index comprehensive evaluation DIFFERENT FEED heavy with female, male pupa.Data statistic analysis employing DPS statistical software carries out variance analysis and significance of difference test.
Experimental result:
Table 1 man-made feeds of the present invention and the Zhou Darong researcher of Plant Protection institute, Chinese Academy of Agricultral Sciences equal new No. 7 feeds of exploitation in 1979 and raise the Ostrinia furnacalis effect relatively
The data declaration of test list 1, feed formula of the present invention raise 3 generation Ostrinia furnacalis male and female pupa compare with new No. 7 feeds that pupa is heavy all to be significantly increased, both significant differences; The egg laying amount of female moth also is significantly increased.
The invention has the beneficial effects as follows that man-made feeds recipe ingredient of the present invention and content thereof are reasonable, nutriment is comprehensive, has improved pupa heavily and the egg laying amount of adult.Due to Chinese herbal medicine is used for the insect artificial diet, help to improve the diseases prevention of larva, resistance against diseases simultaneously.
The specific embodiment
Below in conjunction with specific embodiment, the artificial feedstuff composition of the present invention and preparation thereof are described.
Table 2 Ostrinia furnacalis man-made feeds of the present invention formula and new No. 7 feed formulas
Table 3 Chinese herb feed additive constituent
New No. 7 feed formulas:
Each component of new No. 7 feeds is with reference to table 2, and compound method comprises the steps:
(1) raw material sterilization: put into 120 ℃ of sterilizations of baking oven 2 hours after soy meal, corn flour and brewer's yeast are packed with brown paper, abundant cooling-20 ℃ of refrigerator-freezers preservations of putting into afterwards;
(2) preparation A component: soy meal 120.0g, corn flour 154.0g and brewer's yeast 72.0g part of taking in the first step are put into the raising box, mix, as the A component;
(3) preparation B component: take 12.0g agar, with the dissolving of 100.0ml room temperature distilled water, drop into and contain in the stainless-steel pan of 600.0ml distilled water, boil, be cooled to 60 ℃ as the B component;
(4) preparation C component: take sucrose 60.0g, ascorbic acid 4.0g, erythromycin 0.15g(pulverizes) and sorbic acid 4.0g in a clean beaker, add 300.0ml, 40 ℃ of distilled water to dissolve, as the C component;
(5) measure the formalin 1.6ml of 35%-42% as the E component
(6) component C and E are joined in the B component, be injected in the A component after evenly mixing rapidly, fully mix the room temperature cooled and solidified.
Embodiment 1
Each component of the preparation of man-made feeds is with reference to table 2, and compound method comprises the steps:
(1) raw material sterilization: put into 115 ℃ of sterilizations of baking oven 3 hours after soy meal, corn flour and brewer's yeast are packed with brown paper, abundant cooling-20 ℃ of refrigerator-freezers preservations of putting into afterwards;
(2) preparation A component: soy meal 120.0g, the corn flour 150.0g and the brewer's yeast 40.0g that take in the first step put into the raising box, mix, as the A component;
(3) preparation B component: take 10.0g agar, with the dissolving of 100.0ml room temperature distilled water, drop into and contain in the stainless-steel pan of 600.0ml distilled water, boil, be cooled to 55 ℃ as the B component;
(4) preparation C component: take sucrose 60.0g, ascorbic acid 4.0g, erythromycin 0.15g(pulverizes) and sorbic acid 4.0g in a clean beaker, add 300.0ml, 35 ℃ of distilled water to dissolve, as the C component;
(5) measure Chinese herb feed additive 20.0ml as the D component;
(6) measure the formalin 1.6ml of 35%-42% as the E component
(7) component C, D and E are joined in the B component, be injected in the A component after evenly mixing rapidly, fully mix the room temperature cooled and solidified.
Wherein the preparation method of the Chinese herb feed additive in step 5 is as follows, reference table 3:
1) take balloonflower root 50.0g, sweet wormwood 500.0g, Chinese bulbul 150.0g, fleece-flower root 150.0g, hairyvein agrimony 150.0g, gross weight is 1000.0g, at the bottom of after rinsing, uniform spreading is padded on stainless-steel pan;
2) add 4000.0ml distilled water in stainless-steel pan, soak and boiled afterwards in 25 minutes, kept 35 minutes;
3) use the filtered through gauze liquid, be sub-packed in conical flask after slightly cooling, 120 ℃ of sterilizations 20 minutes are standby.
Embodiment 2
(1) raw material sterilization: put into 120 ℃ of sterilizations of baking oven 2 hours after soy meal, corn flour and brewer's yeast are packed with brown paper, abundant cooling-20 ℃ of refrigerator-freezers preservations of putting into afterwards;
(2) preparation A component: soy meal 120.0g, corn flour 150.0g, brewer's yeast 75.0g part of taking in the first step are put into the raising box, mix, as the A component;
(3) preparation B component: take 15.0g agar, with the dissolving of 100.0ml room temperature distilled water, drop into and contain in the stainless-steel pan of 850.0ml distilled water, boil, be cooled to 60 ℃ as the B component;
(4) preparation C component: take sucrose 60.0g, ascorbic acid 4.0g, erythromycin 0.15g(pulverizes) and sorbic acid 4.0g in a clean beaker, add 300.0ml, 40 ℃ of distilled water to dissolve, as the C component;
(5) measure Chinese herb feed additive 30.0ml as the D component;
(6) measure the formalin 1.6ml of 35%-42% as the E component
(7) component C, D and E are joined in the B component, be injected in the A component after evenly mixing rapidly, fully mix the room temperature cooled and solidified.
Wherein the compound method of the herbal liquid medicine in step 5 is as follows:
1) take balloonflower root 100.0g, sweet wormwood 600.0g, Chinese bulbul 50.0g, fleece-flower root 100.0g, hairyvein agrimony 150.0g, gross weight is 1000.0g, at the bottom of after rinsing, uniform spreading is padded on stainless-steel pan;
2) add 4500.0ml distilled water in stainless-steel pan, soak and boiled afterwards in 30 minutes, kept 30 minutes;
3) use the filtered through gauze liquid, be sub-packed in conical flask after slightly cooling, 121 ℃ of sterilizations 20 minutes are standby.
Embodiment 3
(1) raw material sterilization: put into 125 ℃ of sterilizations of baking oven 1 hour after soy meal, corn flour and brewer's yeast are packed with brown paper, abundant cooling-20 ℃ of refrigerator-freezers preservations of putting into afterwards;
(2) preparation A component: soy meal 120.0g, the corn flour 150.0g and the brewer's yeast 80.0g that take in the first step put into the raising box, mix, as the A component;
(3) preparation B component: take 20.0g agar, with the dissolving of 100.0ml room temperature distilled water, drop into and contain in the stainless-steel pan of 1100.0ml distilled water, boil, be cooled to 65 ℃ as the B component;
(4) preparation C component: take sucrose 60.0g, ascorbic acid 4.0g, erythromycin 0.15g(pulverizes) and sorbic acid 4.0g part in a clean beaker, add 300.0ml, 45 ℃ of distilled water to dissolve, as the C component;
(5) measure medium-height grass additive 40.0ml as the D component;
(6) measure the formalin 1.6ml of 35%-42% as the E component
(7) component C, D and E are joined in the B component, be injected in the A component after evenly mixing rapidly, fully mix the room temperature cooled and solidified.
Wherein the preparation method of the Chinese herb feed additive in step 5 is as follows:
1) take balloonflower root 150.0g, sweet wormwood 700.0g, Chinese bulbul 50.0g, fleece-flower root 50.0g, hairyvein agrimony 50.0g, gross weight is 1000.0g, at the bottom of after rinsing, uniform spreading is padded on stainless-steel pan;
2) add 5000.0ml distilled water in stainless-steel pan, soak and boiled afterwards in 35 minutes, kept 25 minutes;
3) use the filtered through gauze liquid, be sub-packed in conical flask after slightly cooling, 120 ℃ of sterilizations 20 minutes are standby.
Claims (8)
1. Ostrinia furnacalis man-made feeds that contain the Chinese herb feed additive composition, the component that comprises following weight: the formalin 1.6ml of soy meal 120.0g, corn flour 150.0g, sucrose 60.0g, brewer's yeast 40.0-80.0g, ascorbic acid 4.0g, agar 10.0-20.0g, sorbic acid 4.0g, erythromycin 0.15g, 35%-42% and distilled water 1000.0-1500.0ml is characterized in that: also comprise Chinese herb feed additive 20.0-40.0ml; Described Chinese herb feed additive is aqua, is decocted by the component of following weight to make: balloonflower root 50.0-150.0g, sweet wormwood 500.0-700.0g, Chinese bulbul 50.0-150.0g, fleece-flower root 50.0-150.0g, hairyvein agrimony 50.0-150.0g.
2. the compound method that contains the Ostrinia furnacalis man-made feeds of Chinese herb feed additive composition as claimed in claim 1, is characterized in that: comprise the following steps:
(1) raw material sterilization: soy meal, corn flour, brewer's yeast are put into abundant cooling the preservation at-20 ℃ of refrigerator-freezers afterwards of baking oven sterilization;
(2) preparation A component: the various raw materials that take after step (1) is sterilized mix the component as A;
(3) preparation B component: take agar, with the dissolving of 100.0ml distilled water, be added to after dissolving in the container that contains 600.0-1100.0ml distilled water, boil, cooling rear as the B component;
(4) preparation C component: take sucrose, ascorbic acid, erythromycin and sorbic acid, add the distilled water dissolving, as the C component;
(5) the D component is Chinese herb feed additive;
(6) the E component is the formalin of 35%-42%;
(7) component C, D and E are joined in the B component, join after mixing in the A component, fully mix, the room temperature cooled and solidified makes product.
3. the compound method that contains the Ostrinia furnacalis man-made feeds of Chinese herb feed additive composition according to claim 2 is characterized in that:
In described step (1), oven temperature is 115-125 ℃, and sterilization time is 1-3 hour.
4. the compound method that contains the Ostrinia furnacalis man-made feeds of Chinese herb feed additive composition according to claim 2, it is characterized in that: described in step (3), distilled water is room temperature, described chilling temperature is 55-65 ℃.
5. the compound method that contains the Ostrinia furnacalis man-made feeds of Chinese herb feed additive composition according to claim 2, it is characterized in that: described in step (4), distilled water is 300.0ml, 35-45 ℃.
6. the described compound method that contains the Ostrinia furnacalis man-made feeds of Chinese herb feed additive composition of any one according to claim 2-5, it is characterized in that: the preparation of D component described in step (5) comprises the following steps:
1) balloonflower root, sweet wormwood, the Chinese bulbul, the fleece-flower root and hairyvein agrimony are taken according to proportioning, gross weight is 1000.0g;
2) add distilled water, soak, boil liquid;
3) liquid that obtains of step in filtration is sterilized after naturally cooling at a certain temperature, namely gets Chinese herb feed additive.
7. the compound method that contains the Ostrinia furnacalis man-made feeds of Chinese herb feed additive composition according to claim 6, it is characterized in that: step 2) described in water be 4000.0-5000.0ml distilled water, described soak time is 25-35 minute, and described boiling time is 25-35 minute.
8. the compound method that contains the Ostrinia furnacalis man-made feeds of Chinese herb feed additive composition according to claim 6, it is characterized in that: sterilising temp described in step 3) is 120 ℃, sterilization time is 20 minutes.
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Cited By (6)
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| CN103355485A (en) * | 2013-06-27 | 2013-10-23 | 山东省农业科学院植物保护研究所 | Phytase transgenic corn freeze-dried feed and preparation method thereof |
| CN103478493A (en) * | 2013-09-25 | 2014-01-01 | 北京农学院 | Formula of artificial feed for dichocrocis punctiferalis, and manufacturing method of artificial feed |
| CN104522345A (en) * | 2014-11-24 | 2015-04-22 | 桑金全 | Eupolyphoge sinensis feed used for egg-carrying period |
| CN110583913A (en) * | 2019-10-14 | 2019-12-20 | 中国医学科学院药用植物研究所云南分所 | Feed for raising yellow-eared moth and preparation method and application thereof |
| CN112314535A (en) * | 2020-11-27 | 2021-02-05 | 东莞市东阳光冬虫夏草研发有限公司 | Cordyceps sinensis host larva feed and method for feeding cordyceps sinensis host larvae |
| CN114391516A (en) * | 2022-02-18 | 2022-04-26 | 吉林省农业科学院 | Medium for artificial inoculation of Asiatic corn borers in field and preparation method and application thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355485A (en) * | 2013-06-27 | 2013-10-23 | 山东省农业科学院植物保护研究所 | Phytase transgenic corn freeze-dried feed and preparation method thereof |
| CN103478493A (en) * | 2013-09-25 | 2014-01-01 | 北京农学院 | Formula of artificial feed for dichocrocis punctiferalis, and manufacturing method of artificial feed |
| CN104522345A (en) * | 2014-11-24 | 2015-04-22 | 桑金全 | Eupolyphoge sinensis feed used for egg-carrying period |
| CN110583913A (en) * | 2019-10-14 | 2019-12-20 | 中国医学科学院药用植物研究所云南分所 | Feed for raising yellow-eared moth and preparation method and application thereof |
| CN110583913B (en) * | 2019-10-14 | 2022-07-01 | 中国医学科学院药用植物研究所云南分所 | Feed for raising yellow-eared moth and preparation method and application thereof |
| CN112314535A (en) * | 2020-11-27 | 2021-02-05 | 东莞市东阳光冬虫夏草研发有限公司 | Cordyceps sinensis host larva feed and method for feeding cordyceps sinensis host larvae |
| CN112314535B (en) * | 2020-11-27 | 2022-08-12 | 东莞市东阳光冬虫夏草研发有限公司 | Cordyceps sinensis host larva feed and method for feeding cordyceps sinensis host larvae |
| CN114391516A (en) * | 2022-02-18 | 2022-04-26 | 吉林省农业科学院 | Medium for artificial inoculation of Asiatic corn borers in field and preparation method and application thereof |
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| CN103110020B (en) | 2014-02-05 |
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