CN103918729B - A kind of seed dressing and its preparation method and application - Google Patents
A kind of seed dressing and its preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of seed dressing and its preparation method and application, this seed dressing contains the effective ingredient extracted in Anemone Vitifolia, kuh-seng, golden cypress, tarragon, Radix Angelicae Sinensis and thunder godvine, component is simple, easy to use,, noresidue pollution-free to food and cash crops seed, low to person poultry toxicity, and there is the effect of keeping away mouse, desinsection, disinfection and nourishing crop; Its preparation method is simple, flow process is easily controlled, it is easy to operate, and raw material effective ingredient yield is good, and formulation ingredients is stablized, and is easy to preserve and use; Invention provides the using method of described seed dressing, be that it is convenient to implement by rear for described seed dressing dilution and seed mix.
Description
Technical field
The present invention relates to a kind of seed dressing, particularly one is with plant material seed dressing, preparation method and application's technology of this seed dressing.
Background technology
According to late nineteen eighties FAO (Food and Agriculture Organization of the United Nation) statistical data in last century, whole world food and cash crops only because of seed by mouse, damage by disease and insect just make field lack or loss up between 20%-50%, force the annual production seed coat agent in the whole world, seed dressing or seed soaking agent annual production to reach 50-80 ten thousand tons, it is chemical pesticide mainly.Synthesized by their chemical pesticide that primary raw material is all high poison, hypertoxic and residual life is longer, in 1980-2006, they have made certain contribution to agricultural production.At the beginning of 21 century, during human life critical due to chemical pesticide that high poison, severe toxicity and residual life are long, the chemical pesticide long high poison, severe toxicity and residual life is banned by various countries, causes the most of seed coat agent of China, seed dressing is forced to abandon because of raw material producing.
At present, China's agricultural production, to the process (paddy rice) of seed, is with ten thousand clear spirits or strong chlorine oil carries out disinfection, sterilization.They are with trimerization isocyanuric acid, sodium dichloro cyanurate, the environment disinfectant that calcium hypochlorite etc. synthesize for primary raw material.Sterilization is played to crop seeds, for agricultural production and environment disinfected have made eternal exploit.But due to the formation of natural conditions, crops are when mountain region produces, seed is sown into soil will suffer causing harm of mountain region mouse and soil insect, again to bring worm, ovum, germ in seed into, seed is sown in soil will suffer mouse, the causing harm of insect and germ, assures that the sick worm plague of rats of statistics is up to more than 30% according to the United Nations grain farmer.In recent years, make again some mouse, germ, worm (ovum) ensconce in seed or soil, seed to be sowed just suffers the harm of mouse, germ, worm.So, when the ecotope that the mankind depend on for existence is by chemical substance severe contamination and disease, worm, plague of rats serious harm, imperative to the research of environment and the harmless agricultural chemicals (comprising plant resource and biopesticide) of health.
Botanical pesticide is generally divided into three major types: a class is insecticide, and two classes are bactericide, and three classes are seed dressing (comprising seed coat agent and seed soaking agents).Although concerned plant extract has the effect of strong sprout, short root, increasing evil to seed, its research seldom.And formerly study (seed coat agent) in nature strong sprout, short root, increase evil sterilization, sterilization, keep away the effect of mouse insect protected and not obvious, but plant still be it is believed that it is the best source of chemical synthetic pesticide substitute.It is won in history as far back as Early Han Dynasty's period (middle record selected to read by the agriculture book before more than 2000 year) famous agronomist Pan, by the life of government, kind of a technology of urinating " with sheep excrement for main other animal wastes and the Animal Bone juice of adding makes seed dressing through repeatedly process---be referred to as to search kind of a technology " is carried out in Guanzhong area near Chang'an, ancient capital.In recent years, the seed coat agent that China also has biological bacteria, alkaloid and flavone glycoside to synthesize is recorded, but it is subject to impact of temperature, humidity and reduces drug effect, and production can not be widely applied.
Advocating " environment friendly agricultural ", development and today of sustainable agriculture energetically; research and develop a kind of plant resource seed dressing; keep away mouse, disinfection with insect protected, strong sprout urgees root and increasing tiller is imperative, to protection of the environment, promote that agricultural development, guarantee grain security and human body health have profound significance.
Summary of the invention
An object of the present invention is to provide a kind of plant resource seed dressing, to food and cash crops seed without poisoning, noresidue, free from environmental pollution and agricultural product, and have in breeding time seed: strong sprout, short root, increase evil, effect that short photosynthesis, insect protected keep away mouse, disinfection, companion's kind of wide spectrum, with low cost.
Plant resource seed dressing provided by the invention, containing the effective ingredient extracted from following raw material, described raw material comprises: Anemone Vitifolia, kuh-seng, golden cypress, tarragon, Radix Angelicae Sinensis and thunder godvine.
Anemone Vitifolia extract killing rate can reach 88.60%, shrubby sophora extract killing rate can reach 86.10%, triperygium wilfordii extractive killing rate can reach the another extract flavone sterilizing rate of 89.68% Anemone Vitifolia can reach 58.91%, phellodendron extract sterilizing rate can reach 72.00%, Folium Artemisiae Argyi extract sterilizing rate can reach 70.30% and have the effect of sterilization, angelica extract can increase plant seed nutrition breeding time, extends embryo growth time 4-5 days, can also strong sprout, short root, increase the effect of evil, short photosynthesis.Plant resource seed dressing provided by the invention adopts that multiple components is composite to be made, and the comprehensive onset of Multiple components has insect protected and keeps away mouse, strong sprout, short root, increases the effect of evil, short photosynthesis, for agricultural foison is had laid a good foundation.The raw material of seed dressing all come from Chinese medicine to person poultry safety, have no side effect.
Preferred: described raw material weight share matches well, comprising Anemone Vitifolia 6-12, kuh-seng 1-2, golden cypress 1-2, tarragon 1-2, Radix Angelicae Sinensis 0.5-1, thunder godvine 0.5-1.
Preferred: described effective ingredient is comprising Anemone Vitifolia alkali, matrine, phellodendrine, wilfordine, flavone glycoside and saponin.The mass fraction of Anemone Vitifolia alkali in seed dressing is 0.12%-0.24%.
Preferred: to calculate by accounting for seed dressing mass percent: Anemone Vitifolia alkali is 0.07%-0.14%, matrine is 0.01%-0.02%, phellodendrine is 0.01%-0.02%, wilfordine is 0.005%-0.01%, flavone glycoside is 0.02%-0.04%, saponin is 0.005%-0.01.
Two of the object of the invention is to provide the method preparing described plant resource seed dressing, comprises the steps:
A, cut-out, fragmentation: 1, raw material Anemone Vitifolia described in segment, the section being cut into 2-3 centimeter length through slicer is stand-by, and 2, broken: raw material described in all the other is stand-by through the fragmentation of 35-50 mesh sieve.
B, extraction: mixed with extraction solvent by raw material, under normal pressure or reduced pressure, when heating mixed material to temperature reaches close to boiling point, and be incubated 1 hour, filter residue, get leaching liquor, leaching liquor realizes by the Chinese medicine extraction equipment of routine, avoids effective ingredient enzymolysis, and a large amount of impurity leaches; Solvent can be impelled to circulate with stirring during lixiviate, to increase concentration difference, improve extracting effect, mixed system pH value is that 5-6 is advisable, and effective ingredient is stablized.
C, concentrated: concentrated under normal pressure or reduced pressure, leaching liquor is to saturated concentrate.
D, extract synthesis: 1 ~ 2% mass percent concentration slowly adding concentrate weight ratio in the concentrate that step c obtains is 37% hydrochloric acid, the molybdic acid of 0.1 ~ 0.2% and the mass percent concentration of 0.2 ~ 0.5% are 85% phosphoric acid, stir, be warming up to liquid again to boil, be incubated 40 minutes-1 hour, stirred once every 20 minutes, stop extracting when crystal appears in surface, obtain seed dressing.
Preferred: in step b, extracting solution is water, and the weight ratio of raw material and extraction solvent is 1.1-1.2:20-25.Water price is cheap to be easy to get, and research confirms, raw water extract seed dressing is effective, and effective recovery rate is high;
Preferred: step b heats mixed material under an increased pressure, extracting effect is best;
Preferred: in steps d, stop extraction adding the acceptable preservative of pharmacology, as Sodium Benzoate, potassium sorbate etc., to extend the medicament pot-life when crystal appears in liquid surface.
Three of the object of the invention extracts the using method for plant resource seed dressing, is the application technology of seed dressing or seed soaking after described seed dressing dilution.
The invention has the beneficial effects as follows: seed dressing component is simple, use safety, pollution-free to food and cash crops seed, without residual hazard, low to person poultry toxicity; Insect protected keeps away mouse, disinfection and tonic effect good, make disease worm not produce resistance, and mix, wide spectrum of soaking seed, various seed energy disinfection, insect protected are kept away to mouse and play good effect.Use rear to seed germination and growth of seedling without negative effect, be easy to preserve, in comparatively large-temperature range, all can keep stable, and with low cost, be expected to alternative traditional chemical seed dressing and be widely used in organic farming and green agriculture is produced.The simple environmental protection of preparation method provided by the invention, condition is easily controlled, and material composition recovery rate is good, and obtained formulation ingredients is stablized, and is easy to preserve and use.
Embodiment
Specific embodiment:
Below in conjunction with specific embodiment, the solution of the present invention is further elaborated, should be appreciated that specific embodiment only for illustration of technical solution of the present invention, and unrestricted.
Embodiment 1-3
Prepare seed dressing:
A, to get raw material in ratio listed by table 1 broken and to be cut into the section of 2 centimeter length for subsequent use through 35 mesh sieves.
B, weight share are got the raw material made in 1 part of a step and are mixed with 20 parts of water, and in loading cooker, also heating under reduced pressure is incubated lixiviate one hour after liquid boils, and filter, waste, gets leaching liquor for subsequent use;
C, leaching liquor being loaded in concentration tank saturated in being concentrated into, obtaining concentrate;
1% mass percent concentration slowly adding concentrate weight ratio in d, the concentrate that obtains to step c is 37% hydrochloric acid, the molybdic acid of 0.1% and the mass percent concentration of 0.2% are phosphoric acid, stir, be warming up to liquid again to boil, be incubated 40 minutes, stirred once every 20 minutes, stop extracting when crystal appears in surface, add Sodium Benzoate 0.12%, obtain seed dressing.
Parts by weights meter, the composition of raw material described in each embodiment a is as table one:
Table one:
| Embodiment | Anemone Vitifolia | Kuh-seng | Golden cypress | Tarragon | Radix Angelicae Sinensis | Thunder godvine |
| Embodiment 1 | 6 | 1 | 1 | 1 | 0.5 | 0.5 |
| Embodiment 2 | 12 | 2 | 2 | 2 | 0.75 | 0.75 |
| Embodiment 3 | 9 | 1.5 | 1.5 | 1.5 | 1 | 0.5 |
By the detection method analytic product quality in Pharmacopoeia of People's Republic of China and each embodiment products obtained therefrom extract quality mark of detection,
Result is as table two:
Table two:
Embodiment 4-6
Prepare seed dressing:
A, to get raw material in ratio listed by table 1 broken and to be cut into the section of 3 centimeter length for subsequent use through 50 mesh sieves.
B, weight share are got the raw material made in 1 part of a step and are mixed with 30 parts of water, and in loading cooker, also normal heating is incubated lixiviate one hour after liquid boils, and filter, waste, gets leaching liquor for subsequent use;
C, leaching liquor being loaded in concentration tank saturated in being concentrated into, obtaining concentrate;
2% mass percent concentration slowly adding concentrate weight ratio in d, the concentrate that obtains to step c is hydrochloric acid, the molybdic acid of 0.2% and the mass percent concentration of 0.5% are 85% phosphoric acid, stir, be warming up to liquid again to boil, be incubated 1 hour, stirred once every 20 minutes, stop extracting when crystal appears in surface, add potassium sorbate 0.15%, obtain seed dressing.
Parts by weights meter, the composition of raw material described in each embodiment a is as table three:
Table three:
| Embodiment | Anemone Vitifolia | Kuh-seng | Golden cypress | Tarragon | Radix Angelicae Sinensis | Thunder godvine |
| Embodiment 4 | 6 | 1 | 1 | 1 | 0.5 | 0.5 |
| Embodiment 5 | 12 | 2 | 2 | 2 | 0.75 | 0.75 |
| Embodiment 6 | 9 | 1.5 | 1.5 | 1.5 | 1 | 0.5 |
Table four
By the detection method analytic product quality in Pharmacopoeia of People's Republic of China and each embodiment products obtained therefrom extract quality mark of detection,
Result is as table four:
Table four:
Embodiment 7-9
Prepare seed dressing:
A, to get raw material in ratio listed by table 1 broken and to be cut into the section of 3 centimeter length for subsequent use through 42 mesh sieves.
B, weight share are got the raw material made in 1 part of a step and are mixed with 25 parts of water, and loading in cooker and to be heated to be incubated lixiviate one hour after liquid boils under 0.15mpa, filter, waste, gets leaching liquor for subsequent use;
C, leaching liquor being loaded in concentration tank saturated in being concentrated into, obtaining concentrate;
1.5% mass percent concentration slowly adding concentrate weight ratio in d, the concentrate that obtains to step c is 37% hydrochloric acid, the molybdic acid of 0.15% and the mass percent concentration of 0.35% are 85% phosphoric acid, stir, be warming up to liquid again to boil, be incubated 1 hour, stirred once every 20 minutes, stop extracting when crystal appears in surface, add potassium sorbate 0.15%, obtain seed dressing.
Parts by weights meter, the composition of raw material described in each embodiment a is as table five:
Table five:
| Embodiment | Anemone Vitifolia | Kuh-seng | Golden cypress | Tarragon | Radix Angelicae Sinensis | Thunder godvine |
| Embodiment 7 | 6 | 1 | 1 | 1 | 0.5 | 0.5 |
| Embodiment 8 | 12 | 2 | 2 | 2 | 0.75 | 0.75 |
| Embodiment 9 | 9 | 1.5 | 1.5 | 1.5 | 1 | 0.5 |
By the detection method analytic product quality in Pharmacopoeia of People's Republic of China and each embodiment products obtained therefrom extract quality mark of detection,
Result is as table six:
Table six:
Seed dressing compliance test result:
Carry out standardized assays according to Ministry of Agriculture's seed dressing (seed soaking) test rule, the data in following table are results of control treatment.
Comparative test brief summary in seedling stage between the seed dressing wheat paddock of one, system of the present invention
1-1, experiment process and kind of participating in the experiment
1, seed soaking: time less than the ratio seed soaking 4-8 of 1:200 and pesticide-seeds ratio 1:250 in liquid medicine with the seed dressing of system of the present invention.
2, dry seed live (CK): live according to the dry seed of the circulation way of peasant convention.
3, to participate in the experiment kind: the new varieties " river wheat 30 " be bred as with the new variety of wheat system " 96-D, H, 1523 " that this year, Sichuan Agricultural University was bred as and the province academy of agricultural sciences crop of having authorized popularization.
1-2, profile test
1, test site: establish two points, a point is located in the responsibility ground of village nine, sun river of Chinese chestnut a strip of land between hills township, Zizhong County, Sichuan Province society peasant household Jiang Chang thunder, and participate in the experiment kind of " 96-D.H.1523 ".Two points are located in the expensive responsibility ground of society of fiber crops Liuhe village five, Gui De town Zou Chang, participate in the experiment kind of " a river wheat 30 ".
2, test method: Chinese chestnut puts soaked seed 8 hours evening November 10, opened nest sowing on November 11, the dry seed of CK is live,
CK area 1 point of ground, seed soaking sows 4 points of ground, and sowing quantity and fertilizing amount are put on an equal footing.Gui De sowed mid-November, and slightly late a bit, minute is single-row less than brief summary.
3, sampling and measuring: Chinese chestnut puts in sampling and measuring on December 18,37 days breeding times.CK and seed soaking respectively sample 20 strains immediately and measure plant height, individual plant band tiller, main stem leaf age (a few leaf wholeheartedly), and individual plant radical (bar) and the seedling newly five indices such as heavily, measures and provide effect its strong sprout.
1-3, measurement result and effect in seedling stage (seeing attached list)
1, plant height: the average plant height 23.83cm of CK20 strain, seed soaking 20 strain average plant height 22.235cm 1.595CM shorter than CK, short by 6.7%.
2, individual plant band tiller 1.15, seed soaking 20 strain average individual plant band tiller 1.15, seed soaking 29 strain average individual plant band tiller 1.8, many 0.65 tillers of every strain average than CK, increase by 56.5%.
3, main stem leaf age: in CK20 strain four leaves wholeheartedly 1 strain account for 5%, two leaves wholeheartedly 13 strains account for 65%, and three leaves wholeheartedly 6 strains account for
30%; In seed soaking 20 strain four leaves wholeheartedly 16 strains account for 80%, two leaves wholeheartedly 3 strains account for 15%, and three leaves wholeheartedly 1 strain account for 5%.
4, radical (bar): CK20 strain mean elements 8.75, seed soaking 20 strain mean elements 9.75, every strain many 1 average than CK is followed, and increases by 11.4%.
5, shoot root fresh weight: CK20 young plant root fresh weight 22.8g, seed soaking 20 young plant root fresh weight 27.5g, 4.7g heavier than CK, increases by 20.6%.
Several index determinings and indoor measurement tool repeatability above.
Two, the seed dressing of system of the present invention is to the root inducing effect test brief summary in strong sprout of wheat
1, for examination material
The seed dressing of 1-1, system of the present invention is provided by trial (demonstration) plant of plant medicine company research institute, Zizhong County, Sichuan Province.
1-2, wheat breed 1572 are provided by Zizhong County, Sichuan Province seeds company.
2, test method
Wheat Species mixes by the seed dressing of the pesticide-seeds ratio of 1:250 system of the present invention, and enter paper bed morning March 21, do not dress seed clear water CK, and normal temperature laboratory measures, and does not use any means of heating, and normal temperature laboratory is between 12-18 degree Celsius.Seedling, root proterties is measured when waiting until the full exhibition of wheat seeding on April 9 80% liang of leaf after investigation on March 28 germination rate.
3, result of the test
3-1, germination rate: seed dressing and CK are 97%.
3-2, planting percent: Dressing is 93%, CK is 92%, dress seed higher than CK 1 percentage point.
3-3, average individual plant root are long: Dressing 25.75cm, CK24.5cm.Dress seed 1.25cm longer than CK, and long 5.1%.
3-4, average individual plant radical: Dressing 4.87, CK5.38 bar, dresses seed fewer than CK 0.682, and few 12.6%.
3-5, hundred seedling fresh weights: Dressing 27.4g, CK27.2g, dress seed 0.2g heavier than CK, weighs 2.7%
Indoor measurement result table:
4, brief comment
This seed dressing strong sprout, root inducing effect are more obvious, although fewer than CK 0.68 of its radical seed dressing, few 12.6%, but the long 1.25cm longer than CK of root, long 5.1%, prove the more lower bundle of seed dressing its root system of process, water absorbing capacity in soil is stronger, more drought-enduring, more deeply pricks 1.25cm, water-absorption surface is wider, and its drought-resistance ability is just stronger.The wheat seeding of Dressing and root system are all more strong, because height of seedling is than CK height 0.5m, high by 2.7%, hundred careless fresh weights 0.2g heavier than CK, weighs 2.7%, although so the radical of Dressing is a little less, but seedling fresh weight and height of seedling all increase 2.7% than CK, prove that its shoot root is all more strong than CK.Such result shows, this seed dressing can use over a large area.Supporting series green environment-friendly pesticide, the sick worm of bactericidal agent for preventing and treating again during crop growth, then can produce green cereal, veterinary antibiotics etc., for the mankind benefit.Its yield increasing effect measures in the controlling small plot experiment of field.
Three, seed dressing wheat application field of the present invention production test is summed up
1, for examination material
The seed dressing of 1-1, system of the present invention is provided by trial (demonstration) plant of plant medicine company research institute, Zizhong County, Sichuan Province.
1-2, wheat breed 1572 are provided by Zizhong County, Sichuan Province seeds company.
2, indignant condition is tested
2-1, experimental field: select in the responsibility ground of village nine, sun river of Chinese chestnut Ya township, Zizhong County, Sichuan Province society peasant household Jiang Chang thunder.Area 0.4 mu, middle fertility, on the sunny side, surrounding, without concealment, causes harm without birds and beasts physical features.
2-2, experimental scheme: first identify (existing brief summary) root inducing effect in its in indoor, and to dress seed plantation 0.2 mu with 1:250 in field, do not dress seed (CK) plants 0.2 mu and carries out great Qu and compare in strong sprout.
2-3, cultivation management: November 5, code opened nest sowing, adopted 5 chis to open railway carriage or compartment, 2.5*2.5 chi kind 4 row wheat, reserved corn is capable, and mu plants wheat 10,000 nest, every nest sowing 14-15 grain, its this seedling of mu 11-12 ten thousand seedling.Fertilising adopts the end, chases after clear mode together.Ear with green completely pesticide treatment aphid, the first tenday period of a month in May gather in the crops.
2-4, the current year wheat growing stage climatic characteristic: the current year, wheat growing stage (the 11-4 month) rainfall was on the low side, by drought impact, wheat wheat plant becomes short, Tiller ears is affected, November rainfall 22.6mm, December rainfall 11.0mm, January rainfall 6.8mm, February rainfall 3mm, March rainfall 13.7mm, wheat seedling and five months Adult plant 11-3 months co-falling rainfall 57.1mm.Strong wind after April 1 daily rain amount 40.2mm rain, causes wheat large area to lodge, April 13 rainfall 29.7mm again, after rain, wind is larger, and the wheat that April 1 was lodged lodges again, has increased the weight of lodging degree.Fortunately within 3-5 days in advance, ear compared with last year because sky drought affects wheat this year, early April has been Wheat Plant in Late Filling Period, but grain plumpness is also under some influence.
3, test result analysis
3-1, output: paid 50 kilograms of 0.2 mu of Dressing 1:250, folding per mu yield 250 kilograms.Paid 46 kilograms of 0.2 mu of ground that CK does not dress seed, folding per mu yield 230 kilograms.Dress seed than CK every mu volume increase 20 kilograms, volume increase 8.7%.
The real grain of 3-2, fringe and thousand kernel weight: the real grain 37 of Dressing fringe, CK36 grain; Dressing thousand kernel weight 50.5g, CK46.5g, many 1 of the every fringe of Dressing, increases 2.8%; And the many 4g of thousand kernel weight, increase 8.6%
Four, brief comment
In sum, seed dressing, than volume increase 8.7% of not dressing seed, is by a number increasing 2.8%, grain heavily increase 8.6% and come, and these two indexs are also the more lower bundles of the long increase of root 5.1%, more drought resisting and strong sprout effect and established volume increase basis, seed dressing of the present invention can be promoted the use of on large area is produced.
Indoor measurement result table
Output and Major Economic result table
Four, seed dressing of the present invention is to the root inducing effect test brief summary in strong sprout of corn
1, for examination material
The seed dressing of 1-1, system of the present invention.
1-2, corn variety become single 14 to be provided for examination by Zizhong County, Sichuan Province seed.
2, test method
Become in single 14 corns and mix by the pesticide-seeds ratio of the seed dressing 1:300 of system of the present invention, paper bed is entered in March 2 day the morning 10 time, clear water of not dressing seed contrast is CK normal temperature laboratory mensuration, do not use any means of heating, normal temperature laboratory is between 10-15 degree Celsius, add soil after investigation on March 15 germination rate to cover and observe root inducing effect in its in strong sprout, in April 2 corn seedling 80% to reach two leaves be wholeheartedly measure root system.
3, result of the test
3-1, germination rate: Dressing 84%, CK83%, dress seed than CK height one percentage point.
3-2, planting percent: Dressing 64%, CK54%, dress seed than CK high ten percentage points.
3-3, average individual plant root are long: Dressing 82.8cm, CK80.4cm, and dress seed 2.4cm longer than CK, and long 3%.
3-4, average individual plant radical: Dressing 10.75, CK9.7 bar, dresses seed more than CK 1.05, many 10.8%.
3-5, average individual plant seedling fresh weight: seed dressing 1.41g, CK0,926g, dress seed 0.48g heavier than CK, weighs 52.3%
Indoor measurement table
4, brief comment
This seed dressing strong sprout, root inducing effect are obvious, and nuisanceless again, and noresidue, belongs to environmental type agricultural chemicals, is expected to produce green cereal over a large area, green vegetables, green fruit.This series green environment-protection pesticide will be given full play to one's remarkable skill in 21 century, make the green century that 21 century becomes real, for the mankind benefit.
Five, the seed dressing corn application field production test of system of the present invention is summed up
1, for examination material
1-1, seed dressing of the present invention.
1-2, corn variety " one-tenth single 14 " are provided by Zizhong County, Sichuan Province seeds company.
2, profile test
2-1, experimental field: select in the responsibility ground of village nine, sun river of Chinese chestnut a strip of land between hills township, Zizhong County, Sichuan Province society peasant household Jiang Chang thunder.Area 0.4 mu, middle fertility, on the sunny side, surrounding, without concealment, causes harm without poultry physical features, the reserved row of previous crops wheat.
2-2, experimental scheme: first in indoor to its root inducing effect qualification in strong sprout (existing brief summary), and to dress seed plantation 0.2 mu with 1:300 in field, do not dress seed (CK) plants 0.2 mu and carries out great Qu and compare.
2-3, cultivation management: examination ground digs reserved row in late March, March 25, rope code opened nest sowing, and mu plants 1800 nests, 3600 strains, and fertilizer application frequency is chase after at an end three, and July 21 gathered in the crops.
2-4, weather conditions: April in this year rainfall 92.9mm, be beneficial to seedling growth of corn, May rainfall 66.3mm, reduce about 30mm compared with the same period of last year, June, rainfall 103.9mm, reduced 111mm compared with the same period of last year, 5, gas dry days in summer appears in June.Early June rainfall
51.3mm, and overcast and rainy day is more, when at a time when corn take out hero, weave silk, flowering pollinated stage, it is " rainwater fills with smallpox, fills with red palpus " that the saying of peasant is called, and affects corn solid.So the general bald point of corn in this year increases.Strong wind after early July rain, causes corn large area to lodge.
Fortunately it is in the majority to be that root arrives, and seldom fractures.
3, test result analysis
3-1, output: paid 86 kilograms of 0.2 mu of Dressing 1:300, folding per mu yield 430 kilograms.Paid 78 kilograms of 0.2 mu of ground that CK does not dress seed, folding per mu yield 390 kilograms.To dress seed receipts 40 kilogram more than CK every mu, volume increase 10.3%.
The real grain of 3-2, fringe and thousand kernel weight: Dressing fringe examples explain is 466.7, CK424.1 grain, and seed dressing increases by 10.0% than CK; Seed dressing process thousand kernel weight 260g, CK236g, dress seed than CK increasing 10.2%.
In sum: seed dressing is than volume increase 10.3% of not dressing seed, and its based sources increases to some extent in fringe examples explain and thousand kernel weight, and two indexs then derive from strong sprout, root inducing effect becomes positive correlation with yield increasing effect for this.As can be seen here, the seed dressing of system of the present invention can be promoted the use of on large area is produced.
Indoor measurement result table
Output and Major Economic result table
Six, the seed dressing of system of the present invention is to the root inducing effect in strong sprout of rice seedling phase
Indoor measurement experiment brief summary
1, test process and kind of participating in the experiment
1-1, seed soaking: time less than the ratio seed soaking 4-8 of 1:250:200 in medicine, water, kind with the seed dressing of system of the present invention, enter illumination germination insulating box, Observe and measure is the root inducing effect in strong sprout such as root of hair amount, height of seedling altogether.
1-2, clear water (CK) are soaked seed: process the equal water yield with 1 and grain weight compares.
1-3, kind of participating in the experiment: with the new rice variety " II excellent 6078 " of Extend culture in large area.
Seed soaking agent is provided by trial (demonstration) plant of plant pesticide research institute, Zizhong County, Sichuan Province.
Participating in the experiment to plant is provided by Zizhong County, Sichuan Province seeds company.
2, test method
Press seed soaking each parameter during 27 days 13 December in 1999 with medicine, water, kind than seed soaking 100g, soak seed after 7 hours enters paper bed in 20 time, is positioned over illumination germination insulating box.CK enters simultaneously, respectively puts two paper beds, if twice repetition.
3, result of the test
Ru Chuan 5 January 1 in 2000 Tianzhen County is at all, observes when entering bed 7 days on January 3, finds that the root of hair gesture of seed soaking is stronger than CK, and directly encircleed upwards again by its root system after suppressing, CK root system is then more flat, and as in field, it is then strong than CK that it encircles native power.Power-off on January 8 stops constant temperature, makes it grow under normal temperature laboratory, two days later in each random sampling 50 seedling in repeating on January 10 at every turn, namely often processes 100 seedlings, measure three indexs such as its height of seedling, root of hair amount (number), hundred seedling fresh weights and enter to compare.Consequently: CK hundred seedling mean height 6.95cm, average 11.9 of individual plant root of hair amount, hundred seedling fresh weight 8.2g; Seed soaking hundred seedling mean height is 6.66cm, and 0.29cm shorter than CK is short by 4.17%; Average 13.5 of individual plant root of hair amount, increases 13.4% by more than CK 1.6; Fully demonstrate root inducing effect.This is primary root, and with visually observing, its secondary root is obviously more than CK many, just too short, mostly can only under the microscope could number clear.Hundred seedling fresh weight 8.1g, 0.1g lighter than CK are light by 1.2%; According to convention, seed soaking rice shoot is shorter than CK by 4.17%, and its weight also should be light by 4.17%, but reality is only light by 1.2%, and this just reflects that seed soaking rice shoot has the effect in strong sprout of 2.97%.Seed soaking root inducing effect in its then reaches 16.37% in strong sprout.
4, brief comment
In sum, the seed dressing of system of the present invention seed soaking sowing rice shoot can be made to go out seedling is strong, root of hair is fast, well developed root system, metabolism are accelerated, be conducive to rice shoot early growth and quick tiller, cultivate many tillers and strengthen seedling, for paddy rice high yield puts up shelf, lay the foundation.According to observations, seed soaking is the first root of hair of CK comparatively, and rear germination, emerges slower than CK about 1-2 days at variable rate technology, have observe further in field.Seven. seed dressing field of the present invention demonstration brief summary
On the basis of seed dressing indoor measurement of the present invention, can play production-increasing function and the promotion and application in agricultural production thereof for exploring this seed dressing further in agricultural production, on 7500 mu of paddy rice, do 3 demonstrations this year, its result brief summary is in rear.
1, sample plot rice cultivation production overview over the years
Long-term rice cultivation area 8650 mu, based on two sections of seedling, has comparatively small part open country seedling, and long-term seed treatment is all sterilized for main seed soaking with ten thousand disappear peaceful or strong chlorine oils; In annual seedling process, more or less all can occur a part of seedling rot, rotten kind, few some problems such as grade of tillering, causing the phenomenon that Miao Zuhuo by the time looks for rice shoot to plant, is also leaf blast, the multiple district of fringe rice blast, long-term paddy rice per mu yield about 1000 jin.
2, exemplary scenario and process
Demonstration area 7500 mu, distribute 20 villages, demonstration kind " II excellent 838 ", makes up a prescription at random reclaim in the lump when sending out and planting.When breeding, utilize broadcast, meeting repeatedly to peasant household's publicity practical technique and method, namely by every mu of consumption, grain weight medication (5 milliliters every mu, seed consumption jin/2.5 milliliter per jin), seed per jin adds 1 jin of water, and seed soaking 8 was as a child, change water and continue bubble kind, draw after abundance until seed moisture content and sow.After planting manage routinely.
3, demonstration result
Observed by demonstration, compare it with seedling situation over the years and show to be:
3-1, emergence rate are high, emerge neat, without seedling rot phenomenon;
3-2, rice shoot are short strong, tiller early (about 5 days), tiller concentrated, tiller many (more long-term many 1.5-1.8)
3-3, seedling pest and tiller pest obviously comparatively reduce former years;
3-4, owing to tillering early, many, rice shoot is short strong, and formation seedling quality is good;
3-5, root system increase, and grow vigorous, facilitate the formation of seedling quality;
4, brief comment
All there is scarce seedling phenomenon in sample plot, and this year, every household all remained rice shoot over the years, and peasant household reflects: " being what reason? " owing to have employed seed dressing of the present invention seed soaking paddy rice, for increasing production of rice lays the first stone, every mu is increased production about 30 kilograms, is compared by demonstration, and seed dressing of the present invention has promotion budding rate and increases, root system increases, tiller early, concentrate, seedling gesture is short strong, strengthens seedling quality, disinfection, nontoxic novel crop seed soaking, seed dressing.This demonstration conforms to substantially with indoor measurement of the present invention, awaits spread and uses.
Eight, plant pesticide seed dressing wheat seed seed dressing experiment field trial measures brief summary
Seed dressing of the present invention is formed by multiple pure purification of tcm.Based on vegetable alkaloid, there is effect of desinsection, sterilization, nourishing, not containing least bit chemicals composition, pollution-free, without carcinogenic substance, people, animal entrance is nontoxic.For measuring its strong sprout to crops, short root and yield increasing effect, spy arranges this test, to show the field trial measurement result brief summary of wheat seed seed dressing in rear:
1, test process and tested variety
1-1, seed soaking: time less than immersion 8 by 1:200 times of liquid medicine kind.
1-2, contrast clear water are soaked seed 8 hours on an equal basis.
Three repetitions are established, plot area, 0.02 mu, every community, 6 communities in 1-3, field, and by every mu of 20,000 cave planting densities, line-spacing 6 cun is pressed in every community, and 400 caves, apart from 5 cun, are planted in cave.
1-4, kind of participating in the experiment: select 94-335, by every mu of sowing quantity 20 jin of criterion calculation, 0.4 jin, every community.
1-5, seed treatment: soak by community sowing quantity and contrast sowing quantity Unified Set, point community consumption sowing.
1-6, fertilizing amount: conventional amount of application every mu 20 jin, urea, 60 jin, phosphate fertilizer, water excrement 20 is chosen.Every community fertilising 0.4 jin, urea, 1.2 jin, phosphate fertilizer, water excrement 0.4 is chosen, conventional lid kind after fertilising.
1-7, field management: on March 20th, 2000, once, the process dispenser of Dui in April 6 community is once for artificial weeding, with bactericide mu with 50 milliliters, vegetable insecticide mu is with 50 milliliters, and contrast medication oxidation fruit mu is with 80 milliliters, and powder becomes rusty the 100 grams of dispensers of clever mu once.
1-8, described inspection target are temporally, by season, timing is investigated.
1-9, experiment place: responsibility ground (in double-crop field) that society of White Tower village one, dan dish town, Jianyang Xue is kept burning day and night, the husky mud of soil property amount half (previous crop paddy rice), area 0.4 mu.
2, test result analysis
The wheat of 2-1, use seed dressing seed soaking of the present invention, effective fringe 12 fringe, effective fringe 10.76 fringe of contrast, increase by 11.52%, the grain number per spike of seed soaking 49.7, the grain number per spike of contrast 45.4, increases by 9.4%, the thousand kernel weight of seed soaking 42.46 grams, the thousand kernel weight 40.06 grams of contrast, increases by 5.95%, the cell production of process 20.27 jin, the cell production 15.66 jin of contrast, volume increase 29.44%.Amount to per mu yield, the wheat average yield per mu of process 1013.5 jin, the wheat per mu yield of contrast 783 jin, per mu yield increases by 29.44%.
The wheat of 2-2, use seed dressing seed soaking of the present invention, plant height 93.43 centimetres, the plant height of contrast 99.13 centimetres, short by 5.75%; The spike length of seed soaking 10.42 centimetres, the spike length of contrast 9.67 centimetres, increases by 5.87%; The spikelet number of seed soaking 21.56 centimetres, the spikelet number of contrast 19.16 centimetres, increases grain several 12.53%.
Above interpretation of result proves to crops, the seed soaking of plant seed dressing can promote that seedling is neat, effect of strong sprout, short root, resistant to lodging and volume increase.It is the environment friendly agricultural that world today's natural environment ecological balance is praised highly.
3, result of the test table
Seed dressing field trial structure of output table (inspection target) unit of the present invention: mu, gram, jin, centimetre
Nine, seed dressing of the present invention is to target extermination of disease and insect pest effect assessment
1. seed dressing of the present invention is to preventing vertical withered, withered, damping off effect assessment
1.1 reagent agents: green plants seed dressing, in being provided by Sichuan Province, plant medicine company research institute provides.
1.2 for examination pathogen:
Cucumber damping off Pythiumaphanidermatum (Eds.) Fitzp
Cucumber rhizoctonia rot RhizoctoniasolaniKuhn
Cucumber fusarium axysporum Fusariumoxysporum (Schl.) f.sp.CucumerinumOwen
1.3 test methods:
When cucumber seedling grows 1-2 sheet true leaf, by cultured damping off, damping off, it is (dense that Pathogen of Fusarium Wilt is made into spore suspension
Degree is 2x10
5) carry out the inoculation of filling root, often kind of germ process 60 strain.Connect bacterium after 24 hours, fill with root with 200 times of green plants seed dressings
Wherein every 30 strains are root irrigation, and 30 strains are contrast), filled with root once every 5 days, continuous three times.Every day all wants moisturizing at dusk, the
Connect canopy two day morning to ventilate.When instituting an inquiry after contrast morbidity.
2, investigation index
2.1 thing security surveys: after each dispenser, Continuous Observation is for examination plant, and whether blade produces poisoning, and keeps a record.
Poisoning grade scale:
-be not injured
+ extremely slight
++ mitigated injury
+++ affect plant growth
2.2 effect investigation
Record leaf incidence respectively according to following grade scale, calculate according to disease index, and press column index formulae discovery and go out control efficiency.
In formula: CK
0disease index before the dispenser of-blank district
CK
1disease index after the dispenser of-blank district
Pt
0disease index before the dispenser of-chemicals treatment district
Pt
1disease index after the dispenser of-chemicals treatment district
Insect population decreases rate (%)=(pt
0borer population-pt
1borer population) ÷ pt
0borer population × 100
In formula: CK
0borer population before the dispenser of-blank district
CK
1borer population after the dispenser of-blank district
Pt
0borer population before the dispenser of-chemicals treatment district
Pt
1borer population after the dispenser of-chemicals treatment district
3, result and analysis
3.1. plant seed dressing is to the evaluation of cucumber damping off, damping off, fusarium wilt control efficiency
Green plants seed dressing is to cucumber damping off, damping off, fusarium wilt control efficiency from table 1, and green plants seed dressing is harnessed the Yellow River to anti-
Melon damping off does not show preventive effect, slightly imitates, but show not obvious to preventing and treating fusarium wilt when third time is investigated.And higher control efficiency is shown to damping off, the first investigation preventive effect is the 27.96%, the second investigation preventive effect is 36.18%, and third time investigation imitation reaches 40.93%, reaches
Arrive the object of effective suppression disease development.
Seed dressing of the present invention is to cucumber damping off, damping off, fusarium wilt control efficiency
Seed dressing of the present invention is on the result of the impact of cucumber plant seedling growth
4, crop safety
Use to cucumber plant safety after seed dressing of the present invention, blade face comparatively blank without poisoning.
Four, conclusion and discussion
1. pass through seed dressing of the present invention to the evaluation of target extermination of disease and insect pest effect, better to control vegetables damping off effect, control efficiency is not shown to damping off, fusarium wilt.Shown by the result of the test of the impact on cucumber plant seedling growth, seed coat agent, seed dressing are to promotion plant
Leaf photosynthesis, promotes plant strain growth, strengthens plant growing way tool and has certain effect.
Ten, protection against rodents experiment
1, test process and tested variety
1-1,100g maize seed mixes by the seed dressing of the pesticide-seeds ratio of 1:250 system of the present invention
1-2, contrast 100g maize seed are untreated
2, experimental technique
The above-mentioned maize seed handled well and blank are respectively got 50g, and spaced apart, in same iron wire cylinder mould, has been caught 3 mountain mouse and has been positioned in cage simultaneously in field.
Remaining each 50g is repeated above-mentioned experimental procedure once.
3, result
One week observed result: contrast 100g is all eaten up, and the corn seed of seed dressing process has only been moved 6 by mouse but all do not broken by the teeth.
4, interpretation of result
Keep away mouse successful according to the known seed dressing protection against rodents of the present invention of the above results, reason may be raw material Anemone Vitifolia of the present invention, kuh-seng, golden cypress, tarragon, Radix Angelicae Sinensis, thunder godvine have special smell and taste, therefore there is the effect that protection against rodents keeps away mouse.
Claims (7)
1. a seed dressing, is characterized in that: the raw material be made up of following weight parts forms: Anemone Vitifolia 6-12 part, kuh-seng 1-2 part, golden cypress 1-2 part, tarragon 1-2 part, Radix Angelicae Sinensis 0.5-1 part, thunder godvine 0.5-1 part; The mass fraction of summation in seed dressing that active ingredient comprises Anemone Vitifolia alkali, phellodendrine, matrine, wilfordine, flavone glycoside and saponin is 0.12%-0.24%.
2. a kind of seed dressing according to claim 1, it is characterized in that, calculating by accounting for seed dressing mass percent: Anemone Vitifolia alkali is 0.07%-0.14%, matrine is 0.01%-0.02%, phellodendrine is 0.01%-0.02%, wilfordine is 0.005%-0.01%, flavone glycoside is 0.02%-0.04%, saponin is 0.005%-0.01%.
3. a kind of preparation method of seed dressing according to claim 1 or 2, is characterized in that, comprise the following steps:
A, segment and fragmentation: 1, raw material Anemone Vitifolia described in segment, the section being cut into 2-3 centimetre through slicer is stand-by, and 2, broken: by broken for raw material described in all the other stand-by through 35-50 mesh sieve;
B, solvent extraction: by the raw material after step a process by weight 1:20-30 and leaching liquor solvent even, mixed material is heated to temperature under the condition of normal pressure or decompression to reach or close to being incubated one hour during boiling point, filter residue, extract, and regulate immersion liquid pH value to be 5-6, obtain leaching liquor;
C, concentrated: the leaching liquor that concentration step b obtains under normal pressure or reduced pressure, to saturated, obtains concentrate;
D, extract synthesis: 1 ~ 2% mass percent concentration slowly adding concentrate weight ratio in the concentrate that step c obtains is the hydrochloric acid of 37%, the molybdic acid of 0.1 ~ 0.2% and 0.2 ~ 0.5% mass percent concentration are 85% phosphoric acid, stir, be warming up to liquid again to boil, be incubated 40 minutes-1 hour, stirred once every 20 minutes, stop extracting when crystal appears in surface, obtain seed dressing.
4. the preparation method of a kind of seed dressing according to claim 3, is characterized in that: in step b, and lixiviate liquor is water, and the weight ratio of raw material and lixiviate liquor is: 1.1-1.2:20-25.
5. a kind of preparation method of seed dressing according to claim 3 or 4, is characterized in that: step b heats mixed material under an increased pressure.
6. a kind of preparation method of seed dressing according to claim 3 or 4, it is characterized in that: in steps d, concentrate adds the acceptable preservative of pharmacology.
7. the application of a kind of seed dressing described in claim 1 or 2, is characterized in that, by seed dressing or seed soaking after described seed dressing dilution.
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| CN102217659A (en) * | 2011-04-28 | 2011-10-19 | 刘荣常 | Botanical pesticide and its preparation method and use |
| CN102217661A (en) * | 2011-04-28 | 2011-10-19 | 刘荣常 | Botanical bactericide and preparation method and application thereof |
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| CN1451282A (en) * | 2002-04-15 | 2003-10-29 | 四川省本草生物科技有限公司 | Plant source seed coating agent and preparing method thereof |
| CN102217659A (en) * | 2011-04-28 | 2011-10-19 | 刘荣常 | Botanical pesticide and its preparation method and use |
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