CN103110020B - Asiatic corn borer artificial feed containing Chinese herbal medicine additives and preparation method of artificial feed - Google Patents
Asiatic corn borer artificial feed containing Chinese herbal medicine additives and preparation method of artificial feed Download PDFInfo
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Abstract
The invention discloses an Asiatic corn borer artificial feed containing Chinese herbal medicine additives. The Asiatic corn borer artificial feed comprises the following components by weight: 120.0g of soybean flour, 150.0g of corn flour, 60.0g of sucrose, 40.0g-80.0g of beer yeast, 4.0g of ascorbic acid, 10.0g-20.0g of agar, 4.0g of sorbic acid, 0.15g of erythrocin, 0.6ml of formalin, 1000.0ml-1500.0ml of distilled water and 20.0ml-40.0ml of Chinese herbal medicine additives, wherein the Chinese herbal medicine additives are prepared from the following components by weight: 50.0g-150.0g of balloon flowers, 500.0g-700.0g of southernwoods, 50.0g-150.0g of roots of Chinese pulsatilla, 50.0g-150.0g of fleece-flower roots and 50.0g-150.0g of agrimony. The Asiatic corn borer artificial feed disclosed by the invention is reasonable in prescription and comprehensive in nutrient substances, and capable of enhancing the disease-preventing capacity of the Asiatic corn borer, improving the pupal weight and the egg laying amount of the adult borers and avoiding retrogression of the laboratory population.
Description
Technical field
The present invention relates to a kind of insect feedstuff and compound method thereof containing Chinese herb feed additive composition, be specifically related to a kind of Ostrinia furnacalis man-made feeds and compound method thereof containing Chinese herb feed additive composition.
Background technology
Ostrinia furnacalis Ostrinia furnacalis (Guen é e) is the most important insect of China's corn, is having a strong impact on corn yield and the quality in corn producing region.Efficiently, low toxicity chemical pesticide and novel biopesticide exploitation and transgenic insect-resistant corn breeding are three important research directions of comprehensive regulation Ostrinia furnacalis.Efficiently, the screening of low toxicity chemical pesticide and novel biopesticide is identified with mensuration, transgenic corns insect resistace and breed breeding etc. all needs to grow consistent Ostrinia furnacalis test with worm in a large number, the success or not that the indoors artificial of Ostrinia furnacalis is raised has become that impact is efficient, the restraining factors of low toxicity chemical pesticide, novel biopesticide exploitation and transgenic insect-resistant corn Breeding progress.
It is the new No. 7 man-made feeds (Song Yanying that the Zhou great Rong researcher of Plant Protection institute, Chinese Academy of Agricultral Sciences equals exploitation in 1979 that the indoors artificial of Ostrinia furnacalis is raised the most frequently used formula; Zhou great Rong; He Kang comes. " Ostrinia furnacalis is without research and the application of agar meridic diet " < < plant protection journal > >; 1999,26 (4): 324-328.).This feed formula is in the practice of the mass rearing of Ostrinia furnacalis, and along with raising increasing from generation to generation, the Ostrinia furnacalis population of raising there will be degradation phenomena in various degree, and outstanding behaviours is that pupa heavily reduces with the egg laying amount of adult and reduces.
In above-mentioned feed formula, comprise sorbic acid, formaldehyde and erythromycin, bacterium and the fungi of for feed and polypide, carrying have good control action, but ultra-clean insect box microenvironment also can cause going down of corn borer diseases prevention, resistance against diseases, thereby affect survival and the reproduction of Ostrinia furnacalis, cause pupa heavily to reduce with the egg laying amount of adult and reduce.
Therefore, solve population in insect raising and degenerate, first from insect prevention from suffering from the diseases, set about, comprehensive existing investigative technique, by add medicinal herb components in man-made feeds, strengthens the immunity of insect, strengthen the health care of insect, thereby reach, control the object that population degenerates.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Ostrinia furnacalis man-made feeds containing Chinese herb feed additive composition, feed optimizing of the present invention Ostrinia furnacalis feed ingredient proportioning and scientific and reasonable compound method is provided.
The technical solution used in the present invention is: a kind of Ostrinia furnacalis man-made feeds containing Chinese herb feed additive composition, the component that comprises following weight: the formalin 1.6ml of soy meal 120.0g, corn flour 150.0g, sucrose 60.0g, brewer's yeast 40.0-80.0g, ascorbic acid 4.0g, agar 10.0-20.0g, sorbic acid 4.0g, erythromycin 0.15g, 35%-42% and distilled water 1000.0-1500.0ml, also comprise Chinese herb feed additive 20.0-40.0ml; Described Chinese herb feed additive is aqua, is decocted and is made: balloonflower root 50.0-150.0g, sweet wormwood 500.0-700.0g, Chinese bulbul 50.0-150.0g, fleece-flower root 50.0-150.0g, hairyvein agrimony 50.0-150.0g by the component of following weight.
The compound method of the described Ostrinia furnacalis man-made feeds containing Chinese herb feed additive composition, comprises the following steps:
(1) raw material sterilizing: soy meal, corn flour, brewer's yeast are put into abundant cooling the preservation at-20 ℃ of refrigerator-freezers afterwards of baking oven sterilizing;
(2) preparation A component: the various raw materials that take after step (1) sterilizing mix the component as A;
(3) preparation B component: take agar, with 100.0ml distilled water, dissolve, after dissolving, join in the stainless-steel pan that contains 600.0-1100.0ml distilled water, boil, cooling rear as B component;
(4) preparation C component: take sucrose, ascorbic acid, erythromycin and sorbic acid, add distilled water to dissolve, as C component;
(5) D component is Chinese herb feed additive;
(6) formalin that E component is 35%-42%;
(7) component C, D and E are joined in B component, join in A component after mixing, fully mix, room temperature cooled and solidified, makes product.
Described in preferred steps (1), oven temperature is 115-125 ℃, and sterilization time is 1-3 hour.
Described in preferred steps (3), distilled water is room temperature.Described chilling temperature is 55-65 ℃.
Described in preferred steps (4), distilled water is 300.0ml, 35-45 ℃.
The preparation of D component described in preferred steps (5) comprises the following steps:
1) balloonflower root, sweet wormwood, the Chinese bulbul, the fleece-flower root and hairyvein agrimony are taken according to proportioning, gross weight is 1000.0g;
2) add distilled water, soak and boil liquid;
3) liquid that obtains of step in filtration, naturally cooling after sterilizing at a certain temperature, obtain Chinese herb feed additive.
Preferred D component preparation steps 2) described in, water is 4000.0-5000.0ml distilled water, and described soak time is 25-35 minute, and described boiling time is 25-35 minute.
Preferred D component preparation steps 3) described in, sterilising temp is 120 ℃, and sterilization time is 20 minutes.
In order to illustrate that feed formula of the present invention can significantly improve the indexs such as the heavy and Adult worms producting eggs amount of Ostrinia furnacalis male and female pupa, has carried out feeding comparative trial by man-made feeds of the present invention and new No. 7 feeds that the Zhou great Rong researcher of Plant Protection institute, Chinese Academy of Agricultral Sciences equals to develop for 1979.
Test material and method are as follows:
For trying worm source: the Overwintering Larvae gathering from field, Gongzhuling of Jilin Province city, the Adult worms producting eggs obtaining after termination of diapause, through the population of indoors artificial feed captive breeding;
For examination feed: adopt the prepared feed of the embodiment of the present invention two and according to the new No. 7 prepared feeds of filling a prescription;
Test method: raise respectively Ostrinia furnacalis with two kinds of feeds under identical raising box and raising condition, each is raised box and connects 40, corn borer pieces of an egg, and every kind of feed raised 3 boxes is that 3 tests repeat.Whole test was raised for 3 generations continuously.To respectively repeat resulting pupa and differentiate male and female, and get respectively each 20 of female, male pupas and weigh.Then after female, the male pupa that each feed 3 times is repeated to gained mixes respectively, therefrom get at random each 150 of female, male pupas, put into altogether 3 cages of laying eggs, each each 50 of cage male and female pupa of laying eggs.Above cage, lay stencil paper is one, lays eggs while starting wait sprouting wings, and every day, piece number that record is laid eggs, calculated every average egg laying amount.The feeding effect of last and egg laying amount 3 index comprehensive evaluation DIFFERENT FEED heavy with female, male pupa.Data statistic analysis adopts DPS statistical software to carry out variance analysis and significance of difference test.
Experimental result:
Table 1 man-made feeds of the present invention and the Zhou great Rong researcher of Plant Protection institute, Chinese Academy of Agricultral Sciences equal new No. 7 feeds of exploitation in 1979 and raise the comparison of Ostrinia furnacalis effect
The data declaration of test list 1, feed formula of the present invention raise 3 generation Ostrinia furnacalis male and female pupa compare with new No. 7 feeds that pupa is heavy to be all significantly increased, both significant differences; The egg laying amount of female moth is also significantly increased.
The invention has the beneficial effects as follows that man-made feeds recipe ingredient of the present invention and content thereof are reasonable, nutriment is comprehensive, has improved pupa heavily and the egg laying amount of adult.Due to Chinese herbal medicine is used for to insect artificial diet, contribute to improve the diseases prevention of larva, resistance against diseases simultaneously.
The specific embodiment
Below in conjunction with specific embodiment, the artificial feedstuff composition of the present invention and preparation thereof are described.
Table 2 Ostrinia furnacalis man-made feeds formula of the present invention and new No. 7 feed formulas
Table 3 Chinese herb feed additive constituent
New No. 7 feed formulas:
Each component of new No. 7 feeds is with reference to table 2, and compound method comprises the steps:
(1) raw material sterilizing: put into 120 ℃ of sterilizings of baking oven 2 hours after soy meal, corn flour and brewer's yeast are packed with brown paper, abundant cooling-20 ℃ of refrigerator-freezers preservations of putting into afterwards;
(2) preparation A component: soy meal 120.0g, corn flour 154.0g and brewer's yeast 72.0g part of taking in the first step are put into raising box, mix, as A component;
(3) preparation B component: take 12.0g agar, dissolve with 100.0ml room temperature distilled water, drop in the stainless-steel pan that contains 600.0ml distilled water, boil, be cooled to 60 ℃ as B component;
(4) preparation C component: take sucrose 60.0g, ascorbic acid 4.0g, erythromycin 0.15g(pulverizes) and sorbic acid 4.0g in a clean beaker, add 300.0ml, 40 ℃ of distilled water dissolvings, as C component;
(5) the formalin 1.6ml that measures 35%-42% is as E component
(6) component C and E are joined in B component, after evenly mixing rapidly, be injected in A component, fully mix room temperature cooled and solidified.
Embodiment 1
Each component of the preparation of man-made feeds is with reference to table 2, and compound method comprises the steps:
(1) raw material sterilizing: put into 115 ℃ of sterilizings of baking oven 3 hours after soy meal, corn flour and brewer's yeast are packed with brown paper, abundant cooling-20 ℃ of refrigerator-freezers preservations of putting into afterwards;
(2) preparation A component: soy meal 120.0g, the corn flour 150.0g and the brewer's yeast 40.0g that take in the first step put into raising box, mix, as A component;
(3) preparation B component: take 10.0g agar, dissolve with 100.0ml room temperature distilled water, drop in the stainless-steel pan that contains 600.0ml distilled water, boil, be cooled to 55 ℃ as B component;
(4) preparation C component: take sucrose 60.0g, ascorbic acid 4.0g, erythromycin 0.15g(pulverizes) and sorbic acid 4.0g in a clean beaker, add 300.0ml, 35 ℃ of distilled water dissolvings, as C component;
(5) measure Chinese herb feed additive 20.0ml as D component;
(6) the formalin 1.6ml that measures 35%-42% is as E component
(7) component C, D and E are joined in B component, after evenly mixing rapidly, be injected in A component, fully mix room temperature cooled and solidified.
Wherein the preparation method of the Chinese herb feed additive in step 5 is as follows, reference table 3:
1) take balloonflower root 50.0g, sweet wormwood 500.0g, Chinese bulbul 150.0g, fleece-flower root 150.0g, hairyvein agrimony 150.0g, gross weight is 1000.0g, at the bottom of after rinsing, uniform spreading is padded on stainless-steel pan;
2) in stainless-steel pan, add 4000.0ml distilled water, soak and boil afterwards for 25 minutes, keep 35 minutes;
3) use filtered through gauze liquid, be sub-packed in conical flask after slightly cooling, 120 ℃ of sterilizings 20 minutes are standby.
Embodiment 2
(1) raw material sterilizing: put into 120 ℃ of sterilizings of baking oven 2 hours after soy meal, corn flour and brewer's yeast are packed with brown paper, abundant cooling-20 ℃ of refrigerator-freezers preservations of putting into afterwards;
(2) preparation A component: soy meal 120.0g, corn flour 150.0g, brewer's yeast 75.0g part of taking in the first step are put into raising box, mix, as A component;
(3) preparation B component: take 15.0g agar, dissolve with 100.0ml room temperature distilled water, drop in the stainless-steel pan that contains 850.0ml distilled water, boil, be cooled to 60 ℃ as B component;
(4) preparation C component: take sucrose 60.0g, ascorbic acid 4.0g, erythromycin 0.15g(pulverizes) and sorbic acid 4.0g in a clean beaker, add 300.0ml, 40 ℃ of distilled water dissolvings, as C component;
(5) measure Chinese herb feed additive 30.0ml as D component;
(6) the formalin 1.6ml that measures 35%-42% is as E component
(7) component C, D and E are joined in B component, after evenly mixing rapidly, be injected in A component, fully mix room temperature cooled and solidified.
Wherein the compound method of the herbal liquid medicine in step 5 is as follows:
1) take balloonflower root 100.0g, sweet wormwood 600.0g, Chinese bulbul 50.0g, fleece-flower root 100.0g, hairyvein agrimony 150.0g, gross weight is 1000.0g, at the bottom of after rinsing, uniform spreading is padded on stainless-steel pan;
2) in stainless-steel pan, add 4500.0ml distilled water, soak and boil afterwards for 30 minutes, keep 30 minutes;
3) use filtered through gauze liquid, be sub-packed in conical flask after slightly cooling, 121 ℃ of sterilizings 20 minutes are standby.
Embodiment 3
(1) raw material sterilizing: put into 125 ℃ of sterilizings of baking oven 1 hour after soy meal, corn flour and brewer's yeast are packed with brown paper, abundant cooling-20 ℃ of refrigerator-freezers preservations of putting into afterwards;
(2) preparation A component: soy meal 120.0g, the corn flour 150.0g and the brewer's yeast 80.0g that take in the first step put into raising box, mix, as A component;
(3) preparation B component: take 20.0g agar, dissolve with 100.0ml room temperature distilled water, drop in the stainless-steel pan that contains 1100.0ml distilled water, boil, be cooled to 65 ℃ as B component;
(4) preparation C component: take sucrose 60.0g, ascorbic acid 4.0g, erythromycin 0.15g(pulverizes) and sorbic acid 4.0g part in a clean beaker, add 300.0ml, 45 ℃ of distilled water dissolvings, as C component;
(5) measure medium-height grass additive 40.0ml as D component;
(6) the formalin 1.6ml that measures 35%-42% is as E component
(7) component C, D and E are joined in B component, after evenly mixing rapidly, be injected in A component, fully mix room temperature cooled and solidified.
Wherein the preparation method of the Chinese herb feed additive in step 5 is as follows:
1) take balloonflower root 150.0g, sweet wormwood 700.0g, Chinese bulbul 50.0g, fleece-flower root 50.0g, hairyvein agrimony 50.0g, gross weight is 1000.0g, at the bottom of after rinsing, uniform spreading is padded on stainless-steel pan;
2) in stainless-steel pan, add 5000.0ml distilled water, soak and boil afterwards for 35 minutes, keep 25 minutes;
3) use filtered through gauze liquid, be sub-packed in conical flask after slightly cooling, 120 ℃ of sterilizings 20 minutes are standby.
Claims (5)
1. Ostrinia furnacalis man-made feeds containing Chinese herb feed additive composition, the component that comprises following weight: the formalin 1.6ml of soy meal 120.0g, corn flour 150.0g, sucrose 60.0g, brewer's yeast 40.0-80.0g, ascorbic acid 4.0g, agar 10.0-20.0g, sorbic acid 4.0g, erythromycin 0.15g, 35%-42% and distilled water 1000.0-1500.0ml, is characterized in that: also comprise Chinese herb feed additive 20.0-40.0ml; Described Chinese herb feed additive is aqua, is decocted and is made: balloonflower root 50.0-150.0g, sweet wormwood 500.0-700.0g, Chinese bulbul 50.0-150.0g, fleece-flower root 50.0-150.0g, hairyvein agrimony 50.0-150.0g by the component of following weight
The preparation of wherein said Chinese herb feed additive comprises the following steps:
1) balloonflower root, sweet wormwood, the Chinese bulbul, the fleece-flower root and hairyvein agrimony are taken according to proportioning, gross weight is 1000.0g;
2) add distilled water, soak, boil liquid;
3) liquid that obtains of step in filtration, naturally cooling after sterilizing at a certain temperature, obtain Chinese herb feed additive;
Above-mentioned steps 2) water described in is 4000.0-5000.0ml distilled water, and described soak time is 25-35 minute, and described boiling time is 25-35 minute;
Above-mentioned steps 3) sterilising temp described in is 120 ℃, and sterilization time is 20 minutes.
2. the compound method of the Ostrinia furnacalis man-made feeds containing Chinese herb feed additive composition as claimed in claim 1, is characterized in that: comprise the following steps:
(1) raw material sterilizing: soy meal, corn flour, brewer's yeast are put into abundant cooling the preservation at-20 ℃ of refrigerator-freezers afterwards of baking oven sterilizing;
(2) preparation A component: the various raw materials that take after step (1) sterilizing mix the component as A;
(3) preparation B component: take agar, with 100.0ml distilled water, dissolve, after dissolving, be added in the container that contains 600.0-1100.0ml distilled water, boil, cooling rear as B component;
(4) preparation C component: take sucrose, ascorbic acid, erythromycin and sorbic acid, add distilled water to dissolve, as C component;
(5) D component is Chinese herb feed additive;
(6) formalin that E component is 35%-42%;
(7) component C, D and E are joined in B component, join in A component after mixing, fully mix, room temperature cooled and solidified, makes product.
3. the compound method of the Ostrinia furnacalis man-made feeds containing Chinese herb feed additive composition according to claim 2, is characterized in that:
In described step (1), oven temperature is 115-125 ℃, and sterilization time is 1-3 hour.
4. the compound method of the Ostrinia furnacalis man-made feeds containing Chinese herb feed additive composition according to claim 2, is characterized in that: described in step (3), distilled water is room temperature, and described chilling temperature is 55-65 ℃.
5. the compound method of the Ostrinia furnacalis man-made feeds containing Chinese herb feed additive composition according to claim 2, is characterized in that: described in step (4), distilled water is 300.0ml, 35-45 ℃.
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| CN103355485A (en) * | 2013-06-27 | 2013-10-23 | 山东省农业科学院植物保护研究所 | Phytase transgenic corn freeze-dried feed and preparation method thereof |
| CN103478493B (en) * | 2013-09-25 | 2014-11-26 | 北京农学院 | Formula of artificial feed for dichocrocis punctiferalis, and manufacturing method of artificial feed |
| CN104522345A (en) * | 2014-11-24 | 2015-04-22 | 桑金全 | Eupolyphoge sinensis feed used for egg-carrying period |
| CN110583913B (en) * | 2019-10-14 | 2022-07-01 | 中国医学科学院药用植物研究所云南分所 | Feed for raising yellow-eared moth and preparation method and application thereof |
| CN112314535B (en) * | 2020-11-27 | 2022-08-12 | 东莞市东阳光冬虫夏草研发有限公司 | Cordyceps sinensis host larva feed and method for feeding cordyceps sinensis host larvae |
| CN114391516B (en) * | 2022-02-18 | 2023-03-17 | 吉林省农业科学院 | Medium for artificial inoculation of Asiatic corn borers in field and preparation method and application thereof |
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| CN1160494A (en) * | 1997-01-27 | 1997-10-01 | 中国农业科学院植物保护研究所 | Feed for large-scale raising of corn borer |
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