CN105146229A - Rice stem borer larva artificial feed and preparation method and application thereof - Google Patents
Rice stem borer larva artificial feed and preparation method and application thereof Download PDFInfo
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- CN105146229A CN105146229A CN201510729465.5A CN201510729465A CN105146229A CN 105146229 A CN105146229 A CN 105146229A CN 201510729465 A CN201510729465 A CN 201510729465A CN 105146229 A CN105146229 A CN 105146229A
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- 241000426497 Chilo suppressalis Species 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 76
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- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 43
- 235000009566 rice Nutrition 0.000 claims abstract description 43
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 36
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 18
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 18
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 18
- 229930006000 Sucrose Natural products 0.000 claims abstract description 18
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 18
- 229960004793 sucrose Drugs 0.000 claims abstract description 18
- 239000005018 casein Substances 0.000 claims abstract description 16
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims abstract description 16
- 235000021240 caseins Nutrition 0.000 claims abstract description 16
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims abstract description 14
- 235000019743 Choline chloride Nutrition 0.000 claims abstract description 14
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims abstract description 14
- 229960003178 choline chloride Drugs 0.000 claims abstract description 14
- 229930003231 vitamin Natural products 0.000 claims abstract description 14
- 235000013343 vitamin Nutrition 0.000 claims abstract description 14
- 239000011782 vitamin Substances 0.000 claims abstract description 14
- 229940088594 vitamin Drugs 0.000 claims abstract description 14
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 14
- 229920001817 Agar Polymers 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 10
- 239000008272 agar Substances 0.000 claims abstract description 10
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000010199 sorbic acid Nutrition 0.000 claims abstract description 10
- 229940075582 sorbic acid Drugs 0.000 claims abstract description 10
- 239000004334 sorbic acid Substances 0.000 claims abstract description 10
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 9
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 9
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000011718 vitamin C Substances 0.000 claims abstract description 9
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 9
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000012153 distilled water Substances 0.000 claims abstract description 5
- 235000012054 meals Nutrition 0.000 claims description 14
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- 229930027917 kanamycin Natural products 0.000 abstract 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 abstract 1
- 229960000318 kanamycin Drugs 0.000 abstract 1
- 229930182823 kanamycin A Natural products 0.000 abstract 1
- 235000010469 Glycine max Nutrition 0.000 description 6
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
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- Fodder In General (AREA)
Abstract
The invention discloses rice stem borer larva artificial feed and a preparation method and application thereof. The feed is prepared from, by mass, 3.96-4.52% of rice stem powder, 1.97-2.26% of rice chaff powder, 0.58-0.68% of yeast powder, 2.31-2.71% of casein, 1.16-1.36% of cane sugar, 0.23-0.27% of vitamin C, 0.12-0.14% of mixed vitamin, 0.12-0.14% of vitamin E, 0.06-0.07% of cholesterol, 0.06-0.07% of choline chloride, 0.52-0.54% of sorbic acid, 0.17-0.23% of kanamycin, 1.97-2.26% of agar powder and the balance distilled water. Raw materials of the feed are easy to obtain. The preparation process is simple, the quantity of insects bred per unit is large, growth is uniform, and bioassay and other biologic tests are convenient.
Description
Technical field
The present invention relates to the man-made feeds of a kind of insect, specifically, relate to a kind of rice-stem borer larva artificial feed its preparation method and application.
Background technology
Striped rice borer (ChilosuppressalisWalker) belongs to Lepidoptera Pyralidae, be commonly called as " borer ", caused harm by larva moth stem, cause the symptom such as paddy rice " the withered heart ", " dead ears ", be one of important pests in China's Rice Production, area of causing harm every year is very huge with loss.Raise that genetic background in enormous quantities is consistent, to grow neat Chilo spp larvae be the basis of carrying out the researchs such as ecology, toxicity, behavior, indoor biometrics all the year round.Domestic about mainly using rice seedling, rice stem or other natural hosts (as water chestnut, wild rice stem, corn etc.) to raise rice-stem borer in the breeding research of rice-stem borer at present, and natural host raising has the weak point such as easy corruption, cost of labor height; Also research is had to be raise by semi-artificial or artificial formula forage, wherein most of formula forage batching is many, has the hybrid modes such as rice stem powder+soy meal, rice stem powder+wild rice stem powder, rice stem powder+corn flour, and process for preparation is still aobvious loaded down with trivial details, thus feeding cost is high, its application is by restriction to a certain extent; Moreover, from Chilo spp larvae population genetic background angle, obviously contribute to the purifying of population as main material with single host.Therefore still need optimize further rice-stem borer artificial feeding formula and method and research and develop.
Summary of the invention
In order to overcome the defect existed in prior art, the present invention proposes a kind of rice-stem borer larva artificial feed its preparation method and application.Its technical scheme is as follows:
A kind of rice-stem borer larva artificial feed, its each component and mass percent thereof are:
Rice stem powder: 3.96-4.52%;
Rice husk meal: 1.97-2.26%;
Dusty yeast: 0.58-0.68%;
Casein: 2.31-2.71%;
Sucrose: 1.16-1.36%;
Vitamin C: 0.23-0.27%;
Mixed vitamin: 0.12-0.14%;
Vitamin E: 0.12-0.14%;
Cholesterol: 0.06-0.07%;
Choline Chloride: 0.06-0.07%;
Sorbic acid: 0.52-0.54%;
Card Na mycin: 0.17-0.23%;
Agar powder: 1.97-2.26%;
Distilled water: surplus.
The present invention also provides the preparation method of above-mentioned rice-stem borer larva meridic diet, and described rice-stem borer larva meridic diet follows these steps to preparation:
1) weighed agar powder (agar plays freezing action) according to formula, adding water to heat to 100 DEG C on electromagnetic oven boils, and makes it fully melt;
2) rice stem powder, rice husk meal, dusty yeast, casein, sucrose, mixed vitamin, vitamin C, vitamin E, Choline Chloride, cholesterol, sorbic acid, card Na mycin has been weighed (wherein according to formula, rice stem powder, rice husk meal, dusty yeast, sucrose, casein are main nutrient composition, provide carbohydrate, protein and dietary fiber; Choline Chloride, cholesterol, B B-complex, injection Vitamin B_6 are auxiliary element, and it regulates the effect of metabolism, pre-disease prevention; Sorbic acid, card Na mycin play antisepsis) Homogeneous phase mixing is former powder;
3) treat step 1) in liquid cools to 65 DEG C, add step 2) in the former powder of mixing, stir, be then distributed in multiple Cans, in each Cans pour feed into thickness be 2cm-3cm; Cover can bottleneck with black cotton, put into 4 DEG C of refrigerators, refrigerate for subsequent use.
Preferably, wherein said rice stem powder is that rice tillering contains phase rice stem in 80 DEG C of oven dry, grinds, crosses 80 mesh sieves, with the lower dry powder of sieve.Wherein said rice husk meal be fresh paddy in 80 DEG C of oven dry, grind, cross 80 mesh sieves, with the lower dry powder of sieve.
Preferably, wherein said casein powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
Preferably, wherein said cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve.
Preferably, wherein said cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
Preferably, the granularity of wherein said Choline Chloride was the particle of 16 mesh sieves.
Preferably, the granularity of wherein said mixed vitamin is 60 orders (multivitamin and minerals tablet grinds, and crosses 60 mesh sieves, with the lower dry powder of sieve).
Preferably, the volume of wherein said Cans is 500 ~ 750cm
3.
The present invention also provides the artificial formula forage of a kind of above-mentioned rice-stem borer larva raising the application in rice-stem borer larva simultaneously.Rice-stem borer larva raising condition is 26 ± 2 DEG C of temperature, 85% relative humidity, 16L:8D illumination and 2000lux intensity of illumination.
Rice-stem borer meridic diet composition must comprise the growth course desired nutritional material of rice-stem borer, and the Basic Biological Character such as the feeding habit meeting rice-stem borer.The feed that artificial formula reasonable in design raises rice-stem borer should possess, necessary nutrition and rational nutritive proportion; There is the composition stimulating appetite and be conducive to rice-stem borer growth; There is the effective ingredient of preventing and curing diseases; There is good antiseptic effect.Nutrient balance is the key problem of development feed, and the normal growth affecting rice-stem borer is grown by the imbalance of feed nutrition composition.
The growth course of rice-stem borer and life habit have self uniqueness and nutritional need, there is significant difference with the insect of other artificial feeding in nutriment kind and ratio.
The composition that the present invention is directed in feed have developed specific preparation method, and the physical behavior of the effective ingredient in feed and chemical analysis are protected to greatest extent.
The present invention has following beneficial effect:
1, meridic diet raw material is few, and source is easy to get, with low cost;
2, meridic diet preparation procedure is simple, only need get raw material and boil molten;
3, feeding process is simple, easy to operate;
4, unit raises worm amount greatly, grows neat, is convenient to raw survey and waits other biological to test.
Detailed description of the invention
Below in conjunction with detailed description of the invention, technical scheme of the present invention is described in more detail.
Rice-stem borer man-made feeds formula is as shown in table 1, table 2.
Table 1: rice-stem borer man-made feeds formula 1
| Formula components | Mass percent (%) | Quality (g, ml) |
| Rice stem powder | 3.96 | 34 |
| Rice husk meal | 1.97 | 17 |
| Dusty yeast | 0.58 | 5 |
| Casein powder | 2.31 | 20 |
| Cane sugar powder | 1.16 | 10 |
| Mixed vitamin (21 Jin Weita) | 0.12 | 1 |
| Vitamin C | 0.23 | 2 |
| Vitamin E | 0.12 | 1 |
| Choline Chloride | 0.06 | 0.5 |
| Cholesterol | 0.06 | 0.5 |
| Sorbic acid | 0.52 | 4.5 |
| Card Na mycin | 0.17 | 1.5 |
| Agar powder | 1.97 | 17 |
| Distilled water | 86.83 | 750 |
Described artificial feeding rice-stem borer larvae feeds formula 1 follows these steps to preparation: 1) weighed agar powder according to formula, and adding water to heat to 100 DEG C on electromagnetic oven boils, and makes it fully dissolve; 2) weighed rice stem powder, rice husk meal, dusty yeast, casein, sucrose, mixed vitamin, vitamin C, vitamin E, Choline Chloride, cholesterol, sorbic acid, card Na mycin according to formula, Homogeneous phase mixing is former powder; 3) liquid cools to 65 DEG C etc. 1), adds 2) in the former powder of mixing, stir, be then distributed in multiple Cans, in each Cans pour feed into thickness be 2cm-3cm; Cover can bottleneck with black cotton, put into 4 DEG C of refrigerators, refrigerate for subsequent use.
Described rice stem powder be tillering stage fresh rice stem in 60 DEG C of oven dry, grind, cross 80 mesh sieves, with the lower dry powder of sieve.
Described rice husk meal is grind after fresh paddy is dried in 60 DEG C, crosses 80 mesh sieves, with the lower dry powder of sieve.
Described cheese powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
Described cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve.
Described cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
The granularity of described Choline Chloride was the particle (solid) of 16 mesh sieves.
The granularity of described mixed vitamin is that multivitamin and minerals tablet (also claiming Gold Theragran, Shanghai Gui Bao pharmaceutical Co. Ltd) grinds, and crosses 60 mesh sieves, with the lower dry powder of sieve.
The volume of described Cans is 500 ~ 750cm
3.
Table 2: rice-stem borer man-made feeds formula 2
| Formula components | Percentage by weight (%) | Weight (g, ml) |
| Rice stem powder | 3.96 | 34 |
| Soy meal | 1.97 | 17 |
| Dusty yeast | 0.58 | 5 |
| Casein powder | 2.31 | 20 |
| Cane sugar powder | 1.16 | 10 |
| Mixed vitamin (21 Jin Weita) | 0.12 | 1 |
| Vitamin C | 0.23 | 2 |
| Vitamin E | 0.12 | 1 |
| Choline Chloride | 0.06 | 0.5 |
| Cholesterol | 0.06 | 0.5 |
| Sorbic acid | 0.52 | 4.5 |
| Card Na mycin | 0.17 | 1.5 |
| Agar powder | 1.97 | 17 |
| Distilled water | 86.83 | 750 |
Described artificial feeding rice-stem borer larvae feeds formula 2 follows these steps to preparation:
1) weighed agar powder according to formula, adding water to heat to 100 DEG C on electromagnetic oven boils, and makes it fully dissolve;
2) weighed rice stem powder, rice husk meal, dusty yeast, casein, sucrose, mixed vitamin, vitamin C, vitamin E, Choline Chloride, cholesterol, sorbic acid, card Na mycin according to formula, Homogeneous phase mixing is former powder;
3) treat step 1) in liquid cools to 65 DEG C, add step 2) in the former powder of mixing, stir, be then distributed in multiple Cans, in each Cans pour feed into thickness be 2cm-3cm; Cover can bottleneck with black cotton, put into 4 DEG C of refrigerators, refrigerate for subsequent use.
Described rice stem powder be tillering stage fresh rice stem in 60 DEG C of oven dry, grind, cross 80 mesh sieves, with the lower dry powder of sieve.
Described soy meal is grind after commercial soybean is dried in 60 DEG C, crosses 80 mesh sieves, with the lower dry powder of sieve.
Described cheese powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
Described cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve.
Described cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
The granularity of described Choline Chloride was the particle (solid) of 16 mesh sieves.
The granularity of described mixed vitamin is that multivitamin and minerals tablet (also claiming Gold Theragran, Shanghai Gui Bao pharmaceutical Co. Ltd) grinds, and crosses 60 mesh sieves, with the lower dry powder of sieve.
The volume of described Cans is 500 ~ 750cm
3.
Table 3: rice-stem borer man-made feeds formula and fresh rice stem feeding effect contrast table (26 DEG C)
As can be seen from Table 3, supplying under examination borer population same case, rice-stem borer survival rate of larvae is raised higher than natural feed (fresh rice stem) 3-4% with rice-stem borer man-made feeds formula 1 and formula 2, percentage of pupation, eclosion rate are also close with natural feed (fresh rice stem), and namely its feeding effect and natural feed (fresh rice stem) are raised quite.But rice-stem borer man-made feeds formulation operations is simple, saves cultured space, easily control, the large-scale breeding for rice-stem borer larva provides indispensable condition.Fill a prescription in addition and 1,2 to compare, 2 add soybean composition owing to filling a prescription, and in current commercial soybean, transformed variety is a lot, is unfavorable for the population purifying of striped rice borer.
The above; be only the present invention's preferably detailed description of the invention; protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses, the simple change of the technical scheme that can obtain apparently or equivalence are replaced and are all fallen within the scope of protection of the present invention.
Claims (10)
1. a rice-stem borer larva artificial feed, is characterized in that, its each component and mass percent thereof are:
Rice stem powder: 3.96-4.52%;
Rice husk meal: 1.97-2.26%;
Dusty yeast: 0.58-0.68%;
Casein: 2.31-2.71%;
Sucrose: 1.16-1.36%;
Vitamin C: 0.23-0.27%;
Mixed vitamin: 0.12-0.14%;
Vitamin E: 0.12-0.14%;
Cholesterol: 0.06-0.07%;
Choline Chloride: 0.06-0.07%;
Sorbic acid: 0.52-0.54%;
Card Na mycin: 0.17-0.23%;
Agar powder: 1.97-2.26%;
Distilled water: surplus.
2. the preparation method of rice-stem borer larva artificial feed described in a claim 1, it is characterized in that, comprise the following steps: 1) formula has weighed agar powder according to claim 1, adding water to heat to 100 DEG C on electromagnetic oven boils, and makes it fully melt;
2) formula has weighed rice stem powder according to claim 1, rice husk meal, dusty yeast, casein, sucrose, mixed vitamin, vitamin C, vitamin E, Choline Chloride, cholesterol, sorbic acid, card Na mycin Homogeneous phase mixing be former powder;
3) treat step 1) in liquid cools to 65 DEG C, add step 2) described in mix former powder, stir, be then distributed in multiple Cans, in each Cans pour feed into thickness be 2cm-3cm; Cover can bottleneck with black cotton, put into 4 DEG C of refrigerators, refrigerate for subsequent use.
3. the preparation method of rice-stem borer larva artificial feed according to claim 2, it is characterized in that, described rice stem powder is that rice tillering contains phase rice stem in 80 DEG C of oven dry, grind, cross 80 mesh sieves, with the lower dry powder of sieve, described rice husk meal is that fresh paddy is in 80 DEG C of oven dry, grind, cross 80 mesh sieves, with the lower dry powder of sieve.
4. the preparation method of rice-stem borer larva artificial feed according to claim 2, it is characterized in that, described casein powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
5. the preparation method of rice-stem borer larva artificial feed according to claim 2, it is characterized in that, described cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve.
6. the preparation method of rice-stem borer larva artificial feed according to claim 2, it is characterized in that, cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
7. the preparation method of rice-stem borer larva artificial feed according to claim 2, it is characterized in that, the granularity of Choline Chloride was the particle of 16 mesh sieves.
8. the preparation method of rice-stem borer larva artificial feed according to claim 2, it is characterized in that, the granularity of described mixed vitamin is 60 orders.
9. the preparation method of rice-stem borer larva artificial feed according to claim 2, it is characterized in that, the volume of described Cans is 500 ~ 750cm
3.
10. rice-stem borer larva artificial feed described in claim 1 is raising the application in rice-stem borer larva.
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| CN201510729465.5A CN105146229A (en) | 2015-10-30 | 2015-10-30 | Rice stem borer larva artificial feed and preparation method and application thereof |
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| CN201510729465.5A CN105146229A (en) | 2015-10-30 | 2015-10-30 | Rice stem borer larva artificial feed and preparation method and application thereof |
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| CN105146229A true CN105146229A (en) | 2015-12-16 |
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| CN201510729465.5A Pending CN105146229A (en) | 2015-10-30 | 2015-10-30 | Rice stem borer larva artificial feed and preparation method and application thereof |
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| CN106841632A (en) * | 2016-12-24 | 2017-06-13 | 华中农业大学 | The bioassay method that a kind of trans Bt gene crops foreign protein environment withholds |
| CN109156671A (en) * | 2018-10-17 | 2019-01-08 | 溧阳益植生物技术有限公司 | A kind of feed and preparation method thereof of artificial feeding corn borer |
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| CN101507472A (en) * | 2009-03-19 | 2009-08-19 | 中国农业科学院植物保护研究所 | Artificial feedstuff of striped rice borer, preparation method thereof and breeding method |
| CN102356811A (en) * | 2011-09-22 | 2012-02-22 | 中国水稻研究所 | Formula of feed for chilo suppressalis artificial feeding, preparation method of the feed, and large-scale feeding method of chilo suppressalis |
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| CN101507472A (en) * | 2009-03-19 | 2009-08-19 | 中国农业科学院植物保护研究所 | Artificial feedstuff of striped rice borer, preparation method thereof and breeding method |
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|---|---|---|---|---|
| CN106841632A (en) * | 2016-12-24 | 2017-06-13 | 华中农业大学 | The bioassay method that a kind of trans Bt gene crops foreign protein environment withholds |
| CN106841632B (en) * | 2016-12-24 | 2019-01-18 | 华中农业大学 | A kind of bioassay method that trans Bt gene crops foreign protein environment is withheld |
| CN109156671A (en) * | 2018-10-17 | 2019-01-08 | 溧阳益植生物技术有限公司 | A kind of feed and preparation method thereof of artificial feeding corn borer |
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Application publication date: 20151216 |