CN102356811A - Formula of feed for chilo suppressalis artificial feeding, preparation method of the feed, and large-scale feeding method of chilo suppressalis - Google Patents
Formula of feed for chilo suppressalis artificial feeding, preparation method of the feed, and large-scale feeding method of chilo suppressalis Download PDFInfo
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Abstract
The invention discloses a formula of feed for Chilo suppressalis artificial feeding, a preparation method of the feed, and a large-scale feeding method of chilo suppressalis, and belongs to the technical fields of insect artificial feed and large-scale feeding. The large-scale feeding method of chilo suppressalis comprises the following steps of 1, preparing the feed from 600 to 900 parts of distilled water, 12 to 18 parts of agar, 8 to 16 parts of rice stem powder, 40 to 55 parts of cooked soybean powder, 28 to 45 parts of rice chaff powder, 18 to 28 parts of casein, 4 to 9 parts of yeast powder, 8 to 16 parts of cane sugar, 1 to 4 parts of vitamin C1, 0.5 to 3 parts of 21 Jinweita, 0.5 to 3 parts of vitamin E, 0.1 to 1.2 parts of choline chloride, 3 to 8 parts of sorbic acid and 3 to 8 parts of aureomycin, 2, introducing chilo suppressalis egg masses and checking up a rate of hatchability, 3, collecting pupas, and 4, colleting eggs of chilo suppressalis adults. The formula of the feed for Chilo suppressalis artificial feeding, the preparation method of the feed, and the large-scale feeding method of chilo suppressalis have the advantages of simple processes, low cost, labor saving and time saving characteristics, easiness of operation, and good flexibility for large-scale feeding.
Description
Technical field
The invention belongs to insect artificial diet and large-scale breeding technical field, be specially feed formula and the preparation method and extensive method for breeding of striped rice borer artificial feeding.
Background technology
It is one of basic fundamental that entomologizes that man-made feeds are raised insect; Utilize this technology can raise throughout the year and breed the required experiment worm source of scientific research; Because the composition of man-made feeds can artificially be controlled; And can provide genetic background and physiological status close or consistent a large amount of worms source, thereby also be the important method (Wang Yannian etc. 1984) of the Physiology and biochemistry of research insect.Striped rice borer
Chilo suppressalis(Walker) distribute very extensively, all there is generation on ground such as Japan, Philippine, Vietnam, Thailand, Malaysia, India, and the domestic distribution of China is also very extensive, is most important borer pest on China paddy rice.In the integrated control to striped rice borer; The utilization of novel agrochemical exploitation and transgenic paddy rice (Bt paddy rice) plays an important role; And the novel biopesticide exploitation all needs genetic background similar with the biologicall test of Bt paddy rice insect resistace; A large amount of striped rice borer examination worms that development duration is consistent, the unique way that obtains such striped rice borer examination worm is that mass artificial is raised.
The history in existing more than 40 year of the research of striped rice borer man-made feeds; Also reported a collection of artificial feed formula therebetween; But these striped rice borer man-made feeds and method for breeding thereof need manual work be gathered the striped rice borer pupa in feed in feeding process; And raising scale is subject to larva raising space, comparatively labor intensive.Simultaneously because the frequent artificial larva that disturbs is unfavorable for that larva grows up.
Summary of the invention
To the problems referred to above that exist in the prior art; The objective of the invention is to design provides a kind of extensive, the easy of striped rice borer, the feed formula of economic striped rice borer artificial feeding of raising and technical scheme of preparation method and extensive method for breeding of having realized; The artificial difficult problem of labor in the pupa processing procedure during solution is raised; It is easy to have method, price economy, time saving and energy saving, advantages such as easy operating.
The feed formula of described striped rice borer artificial feeding is characterized in that being made up of following substances in parts by weight: distilled water 600-900 part, agar 12-18 part, rice stem powder 8-16 part; Ripe soy meal 40-55 part, rice husk meal 28-45 part, casein 18-28 part, dusty yeast 4-9 part; Sucrose 8-16 part, vitamin C 1-4 part, 21 gold medals are tieed up his 0.5-3 part, vitamin E 0.5-3 part; Choline Chloride 0.1-1.2 part, sorbic acid 3-8 part, aureomycin 3-8 part.
The feed formula of described striped rice borer artificial feeding is characterized in that: distilled water 700-800 part, agar 15-18 part, rice stem powder 10-15 part; Ripe soy meal 45-50 part, rice husk meal 30-40 part, casein 20-25 part, dusty yeast 5-8 part; Sucrose 10-15 part, Catergen-3 part, his 1-2 part of 21 gold medals dimension, vitamin e1-2 part; Choline Chloride 0.3-0.8 part, sorbic acid 4-6 part, aureomycin 2-5 part.
The feed formula of described striped rice borer artificial feeding is characterized in that: distilled water 720-780 part, agar 16-17 part, rice stem powder 12-14 part; Ripe soy meal 46-48 part, rice husk meal 32-35 part, casein 22-24 part, dusty yeast 6-7 part; Sucrose 12-14 part, Catergen .5-2.8 part, 21 gold medals are tieed up his 1.2-1.8 part, vitamin e1 .5-1.8 part; Choline Chloride 0.5-0.7 part, sorbic acid 4.5-5 part, aureomycin 3-4 part.
The feed formula preparation method of described striped rice borer artificial feeding is characterized in that may further comprise the steps:
1) feed formula is divided into A, B, three parts of C, the A part is made up of distillation distilled water 400-500 part, agar 12-18 part; The B part is made up of distilled water 100-200 part, rice stem powder 8-16 part, ripe soy meal 40-55 part, rice husk meal 28-45 part; B part is by his 0.5-3 part of distilled water 100-200 part, casein 18-28 part, dusty yeast 4-9 part, sucrose 8-16 part, vitamin C 1-4 part, 21 gold medals dimension, vitamin E 0.5-3 part, Choline Chloride 0.1-1.2 part, sorbic acid 3-8 part, aureomycin 3-8 part;
2) at first according to prescription weighing good various piece, then with the agar that weighs up and pour in the distilled water, heating and stirring in pot; Agar is dissolved fully, pour the B part of having mixed thoroughly then into, A, B component are fully mixed thoroughly in agitator; Treat to pour the C part into when its temperature drops to 50-58 ° of C, pour in the larva box after mixing thoroughly again, treat that it cools off naturally after; Cover lid places the refrigerator of 3-5 ° of C to preserve subsequent use.
The extensive method for breeding of described striped rice borer artificial feeding is characterized in that may further comprise the steps:
1) making of man-made feeds and preservation: feed formula is divided into A, B, three parts of C, and the A part is made up of distillation distilled water 400-500 part, agar 12-18 part; The B part is made up of distilled water 100-200 part, rice stem powder 8-16 part, ripe soy meal 40-55 part, rice husk meal 28-45 part; B part is by his 0.5-3 part of distilled water 100-200 part, casein 18-28 part, dusty yeast 4-9 part, sucrose 8-16 part, vitamin C 1-4 part, 21 gold medals dimension, vitamin E 0.5-3 part, Choline Chloride 0.1-1.2 part, sorbic acid 3-8 part, aureomycin 3-8 part; At first according to prescription weighing good various piece, then with the agar that weighs up and pour in the distilled water, heating and stirring in pot; Agar is dissolved fully, pour the B part of having mixed thoroughly then into, A, B component are fully mixed thoroughly in agitator; Treat to pour the C part into when its temperature drops to 50-58 ° of C, pour in the larva raising box after mixing thoroughly again, treat that it cools off naturally after; Cover lid places 3-5 ℃ refrigerator to preserve subsequent use;
2) insert the striped rice borer pieces of an egg: the larva raising box that man-made feeds will be housed gets warm again after a cold spell to room temperature naturally; After the rice leaf that will have blackening pieces of an egg is cut; Be suspended on the man-made feeds, the larva of hatching can fall on feed and pierce to get food automatically, and general 1ml feed inserts the striped rice borer ovum of two blackening; Place 26-30 ℃ insectary then, and check incubation rate two days later in the pieces of an egg access;
3) pupa collection: in the time of 20 days, the high instar larvae of survival is transferred to the larva raising box that contains fresh feed at larval growth, generally calculate, and insert the circle of pupating on the top of larva raising box according to the worm amount of every box 180-220 head;
4) become the worm's ovum collection: generally at larval growth during by 30-35 days, the circle of will pupating takes out, and places the adult cage of laying eggs; Place in the cage that a basin is tillered or the paddy rice of jointing stage; Female worm can lay eggs automatically on the rice leaf, and adult mating cage places 25-29 ℃: 20-24 ℃ and the dark laying coop of 12-16 light: 12-8, and the cage of laying eggs is the cylinder mould of 0.5-1 * 0.5-1 * 1 meter size; Change rice seedling every day, promptly sustainable acquisition striped rice borer pieces of an egg;
Repeating an above 1-4 step is sustainable extensive raising striped rice borer.
The extensive method for breeding of described striped rice borer artificial feeding; It is characterized in that described larva raising box adopts cylindrical food crisper commonly used to process its diameter 10-15cm, high 5-7cm; Chiseling diameter on the lid is the 3-5cm opening; Be used for that humidity keeps in the box, and seal, prevent that larva from fleeing from the copper yarn.
The extensive method for breeding of described striped rice borer artificial feeding; It is characterized in that the described circle of pupating adopts the ordinary packing corrugated paper to process; Be cut into wide rectangular of 1.5-2.5cm, be rolled into the paper circle on the placed larva raising box top that size is fit to, and be fixed into circle with pin.
The extensive method for breeding of described striped rice borer artificial feeding; The storage temperature that it is characterized in that refrigerator in the step 1) is 3.5-4.5 ℃; Step 2) temperature of insectary is 27-29 ℃ in; The Wen Guang of laying coop is respectively 26-28 ℃ in the step 4): 21-22 ℃, Light To Dark Ratio is a 13-15 light: 11-9 is dark.
The feed formula of above-mentioned striped rice borer artificial feeding and preparation method and extensive method for breeding, it is easy to have method, price economy, time saving and energy saving, advantages such as easy operating are suitable for extensive, the subculture of standard examination worm, the economic raising.To biology, ecology, the behaviouristics research of striped rice borer, all significant to the research of the relevant biologicall test of striped rice borer and resistance thereof, hereditary mechanism rule.
The percentage composition that relates in the present specification unless otherwise indicated all refers to weight percentage.
The specific embodiment
Understand the present invention for clearer, below through specific embodiment and correlation test the present invention is made further detailed description, these only are the present invention embodiment preferably, but are not limited to these embodiment.
Embodiment 1
1) making of man-made feeds and preservation: feed formula is divided into A, B, three parts of C.The A part is made up of distilled water 500ml, agar 18g; The B part is made up of distilled water 200ml, rice stem powder 15g, ripe soy meal 50g, rice husk meal 40g; C part is by his 1.5g of distilled water 200ml, casein 25g, dusty yeast 8g, sucrose 15g, vitamin C 3g, 21 gold medals dimension, vitamin E 2g, Choline Chloride 0.8g, sorbic acid 6g, aureomycin 5g; At first according to prescription weighing good various piece, then with the agar that weighs up and pour in the distilled water, heating and stirring in pot; Agar is dissolved fully, pour the B part of having mixed thoroughly then into, A, B component are fully mixed thoroughly in agitator; Treat to pour the C part into when its temperature drops to 50 ° of C, pour in the larva raising box after mixing thoroughly again, treat that it cools off naturally after; Cover lid places 3 ℃ refrigerator to preserve subsequent use;
2) insert the striped rice borer pieces of an egg: the larva raising box that man-made feeds will be housed takes out from refrigerator also and gets warm again after a cold spell naturally to room temperature; After the rice leaf that will have blackening pieces of an egg is cut; Be suspended on the man-made feeds, the larva of hatching can fall on feed and pierce to get food automatically, and general 1ml feed inserts the striped rice borer ovum of two blackening; Place 26 ℃ insectary then, and check incubation rate two days later in the pieces of an egg access;
3) pupa collection: in the time of 20 days, the high instar larvae of survival is transferred to the larva raising box that contains fresh feed at larval growth, insert the worm amount and be generally 180 in every box, and insert the circle of pupating on the top of larva raising box;
4) become the worm's ovum collection: generally when larval growth to 30 day, the circle of will pupating takes out, and places the adult cage of laying eggs; Place in the cage that a basin is tillered or the paddy rice of jointing stage; Female worm can lay eggs automatically on the rice leaf, and adult mating cage places 27:21 degree and 16 light: 8 dark laying coops, and the cage of laying eggs is the cylinder mould of 1 * 1 * 1 meter size; Change rice seedling every day, promptly sustainable acquisition striped rice borer pieces of an egg;
Repeating an above 1-4 step is sustainable extensive raising striped rice borer.
Embodiment 2
1) making of man-made feeds and preservation: feed formula is divided into A, B, three parts of C, and the A part is made up of distilled water 400ml, agar 15g; The B part is made up of distilled water 100ml, rice stem powder 10g, ripe soy meal 45g, rice husk meal 30g; C part is by his 1.3g of distilled water 100ml, casein 20g, dusty yeast 5g, sucrose 10g, vitamin C 2g, 21 gold medals dimension, vitamin E 1g, Choline Chloride 0.3g, sorbic acid 4g, aureomycin 2g; At first according to prescription weighing good various piece, then with the agar that weighs up and pour in the distilled water, heating and stirring in pot; Agar is dissolved fully, pour the B part of having mixed thoroughly then into, A, B component are fully mixed thoroughly in agitator; Treat to pour the C part into when its temperature drops to 50 ° of C, pour in the larva raising box after mixing thoroughly again, treat that it cools off naturally after; Cover lid places 4 ℃ refrigerator to preserve subsequent use;
2) insert the striped rice borer pieces of an egg: the larva raising box that man-made feeds will be housed takes out from refrigerator also and gets warm again after a cold spell naturally to room temperature; After the rice leaf that will have blackening pieces of an egg is cut; Be suspended on the man-made feeds, the larva of hatching can fall on feed and pierce to get food automatically, and general 1ml feed inserts the striped rice borer ovum of three blackening; Place 28 ℃ insectary then, and check incubation rate two days later in the pieces of an egg access;
3) pupa collection: in the time of 20 days, the high instar larvae of survival is transferred to the larva raising box that contains fresh feed at larval growth, generally calculate, and insert the circle of pupating on the top of larva raising box according to the worm amount of 180 in every box;
4) become the worm's ovum collection: generally when larval growth to 32 day, the circle of will pupating takes out, and places the adult cage of laying eggs; Place in the cage that a basin is tillered or the paddy rice of jointing stage; Female worm can lay eggs automatically on the rice leaf, and adult mating cage places 27:21 degree and 16 light: 8 dark laying coops, and the cage of laying eggs is the cylinder mould of 0.5 * 0.5 * 1 meter size; Change rice seedling every day, promptly sustainable acquisition striped rice borer pieces of an egg;
Embodiment 3
1) making of man-made feeds and preservation: feed formula is divided into A, B, three parts of C, and the A part is made up of distilled water 450ml, agar 13g; The B part is made up of distilled water 120ml, rice stem powder 11g, ripe soy meal 46g, rice husk meal 31g; C part is by his 1.2g of distilled water 110ml, casein 22g, dusty yeast 6g, sucrose 11g, vitamin C 3g, 21 gold medals dimension, vitamin E 1.3g, Choline Chloride 0.4g, sorbic acid 4.5g, aureomycin 3g; At first according to prescription weighing good various piece, then with the agar that weighs up and pour in the distilled water, heating and stirring in pot; Agar is dissolved fully, pour the B part of having mixed thoroughly then into, A, B component are fully mixed thoroughly in agitator; Treat to pour the C part into when its temperature drops to 50 ° of C, pour in the larva raising box after mixing thoroughly again, treat that it cools off naturally after; Cover lid places 5 ℃ refrigerator to preserve subsequent use;
2) insert the striped rice borer pieces of an egg: the larva raising box that man-made feeds will be housed takes out from refrigerator also and gets warm again after a cold spell naturally to room temperature; After the rice leaf that will have blackening pieces of an egg is cut; Be suspended on the man-made feeds, the larva of hatching can fall on feed and pierce to get food automatically, and general 1ml feed inserts the striped rice borer ovum of two blackening; Place 27 ℃ insectary then, and check incubation rate two days later in the pieces of an egg access;
3) pupa collection: in the time of 20 days, the high instar larvae of survival is transferred to the larva raising box that contains fresh feed at larval growth, generally calculate, and insert the circle of pupating on the top of larva raising box according to the worm amount of 180 in every box;
4) become the worm's ovum collection: generally when larval growth to 35 day, the circle of will pupating takes out, and places the adult cage of laying eggs; Place in the cage that a basin is tillered or the paddy rice of jointing stage; Female worm can lay eggs automatically on the rice leaf, and adult mating cage places 27:21 degree and 16 light: 8 dark laying coops, and the cage of laying eggs is the cylinder mould of 0.5 * 0.5 * 1 meter size; Change rice seedling every day, promptly sustainable acquisition striped rice borer pieces of an egg;
Embodiment 4
1) making of man-made feeds and preservation: feed formula is divided into A, B, three parts of C, and the A part is made up of distilled water 470ml, agar 14g; The B part is made up of distilled water 140ml, rice stem powder 13g, ripe soy meal 48g, rice husk meal 35g; C part is by his 1.5g of distilled water 140ml, casein 23g, dusty yeast 6.5g, sucrose 13g, vitamin C 2.5g, 21 gold medals dimension, vitamin E 1.3g, Choline Chloride 0.6g, sorbic acid 4.7g, aureomycin 8g; At first according to prescription weighing good various piece, then with the agar that weighs up and pour in the distilled water, heating and stirring in pot; Agar is dissolved fully, pour the B part of having mixed thoroughly then into, A, B component are fully mixed thoroughly in agitator; Treat to pour the C part into when its temperature drops to 50 ° of C, pour in the larva raising box after mixing thoroughly again, treat that it cools off naturally after; Cover lid places 3 ℃ refrigerator to preserve subsequent use;
2) insert the striped rice borer pieces of an egg: the larva raising box that man-made feeds will be housed takes out from refrigerator also and gets warm again after a cold spell naturally to room temperature; After the rice leaf that will have blackening pieces of an egg is cut; Be suspended on the man-made feeds, the larva of hatching can fall on feed and pierce to get food automatically, and general 1ml feed inserts the striped rice borer ovum of two blackening; Place 28 ℃ insectary then, and check incubation rate two days later in the pieces of an egg access;
3) pupa collection: in the time of 20 days, the high instar larvae of survival is transferred to the larva raising box that contains fresh feed at larval growth, generally calculate, and insert the circle of pupating on the top of larva raising box according to the worm amount of 180 in every box;
4) become the worm's ovum collection: generally when larval growth to 30 day, the circle of will pupating takes out, and places the adult cage of laying eggs; Place in the cage that a basin is tillered or the paddy rice of jointing stage; Female worm can lay eggs automatically on the rice leaf, and adult mating cage places 27:21 degree and 16 light: 8 dark laying coops, and the cage of laying eggs is the cylinder mould of 0.5 * 0.5 * 1 meter size; Change rice seedling every day, promptly sustainable acquisition striped rice borer pieces of an egg;
Embodiment 5
1) making of man-made feeds and preservation: feed formula is divided into A, B, three parts of C, and the A part is made up of distillation distilled water 480ml, agar 13g; The B part is made up of distilled water 130ml, rice stem powder 14g, ripe soy meal 45g, rice husk meal 30g; C part is by his 3g of distilled water 140ml, casein 18g, dusty yeast 4g, sucrose 16g, vitamin C 4g, 21 gold medals dimension, vitamin E 1.3g, Choline Chloride 1.2g, sorbic acid 4g, aureomycin 3.6g; At first according to prescription weighing good various piece, then with the agar that weighs up and pour in the distilled water, heating and stirring in pot; Agar is dissolved fully, pour the B part of having mixed thoroughly then into, A, B component are fully mixed thoroughly in agitator; Treat to pour the C part into when its temperature drops to 50 ° of C, pour in the larva raising box after mixing thoroughly again, treat that it cools off naturally after; Cover lid places 3 ℃ refrigerator to preserve subsequent use;
2) insert the striped rice borer pieces of an egg: the larva raising box that man-made feeds will be housed takes out from refrigerator also and gets warm again after a cold spell naturally to room temperature; After the rice leaf that will have blackening pieces of an egg is cut; Be suspended on the man-made feeds, the larva of hatching can fall on feed and pierce to get food automatically, and general 1ml feed inserts the striped rice borer ovum of three blackening; Place 28 ℃ insectary then, and check incubation rate two days later in the pieces of an egg access;
3) pupa collection: in the time of 20 days, the high instar larvae of survival is transferred to the larva raising box that contains fresh feed at larval growth, generally calculate, and insert the circle of pupating on the top of larva raising box according to the worm amount of 180 in every box;
4) become the worm's ovum collection: generally when larval growth to 34 day, the circle of will pupating takes out, and places the adult cage of laying eggs; Place in the cage that a basin is tillered or the paddy rice of jointing stage; Female worm can lay eggs automatically on the rice leaf, and adult mating cage places 27:21 degree and 16 light: 8 dark laying coops, and the cage of laying eggs is the cylinder mould of 0.5 * 0.5 * 1 meter size; Change rice seedling every day, promptly sustainable acquisition striped rice borer pieces of an egg;
The larva raising box is processed its diameter 10-15cm, high 5-7cm for cylindrical food crisper commonly used; Chiseling diameter on the lid is the 3-5cm opening, is used for humidity maintenance in the box, and seals with the copper yarn; Prevent that larva from fleeing from, a crisper approximately can obtain 100 above striped rice borer pupas.
Pupate to enclose and adopt common corrugated wrapping to process; Be cut into wide rectangular of 1.5-2.5cm; Be rolled into the paper circle on suitable the placed larva raising box top of size; And be fixed into circle with pin, and the circle of pupating has greatly improved the larvae pupation place, and striped rice borer is paid in the death rate and the manual labor in the stage of pupating when having alleviated artificial feeding greatly; What will have pupa pupates to enclose the adult that places the adult laying coop just can obtain to sprout wings; Place a basin rice seedling in the laying coop and can obtain pieces of an egg; Treat that it is after natural blackening on the rice leaf; Cut in the direct access larva raising box and get final product, thereby avoided artificial writing brush to connect the dead and loaded down with trivial details hand labor of larva that worm causes.
Primary Calculation, with a manual work 30 tapes being set has the striped rice borer pieces of an egg to calculate, and makes 30 box man-made feeds approximately 4 hours consuming time, inserts pieces of an egg and needs 1 hour; Larva with survival in the time of 20 days is transferred in the fresh feed, makes 30 box feeds approximately 4 hours consuming time; Shift high instar larvae and add and pupated to enclose 10 hours consuming time, make 30 and pupated to enclose 2 hours; Add about 3 hours of the sterilization clearance time of supporting worm instrument place.Can obtain 100 pupas with each insect box and calculate, obtain 3000 striped rice borer pupas and only need 24 man-hour/people.The invention still further relates to the circle of pupating of gathering the striped rice borer pupa, pierce automatically during larvae pupation in the circle of pupating, the circle of therefore pupating not only can highly effective gathering, can also effectively protect the individuality of having pupated.
Each component that contains in the feed formula of the present invention all can directly be buied from market, and all components all can long term storage, has effectively solved the stable problem that obtains of component of raising winter, more helps extensive subculture and raises.Component in the feed formula of the present invention is common material, is easy to obtain and preserve, and in whole larval phase, only needs to change man-made feeds, and saved artificial collection pupa grain consumed time, the striped rice borer loss of having avoided artificial collection pupa grain to cause.
Test one: the striped rice borer pupa that gather in the 6th generation of artificial feeding of embodiment 1 and field heavily compares, and the result is as shown in table 1.
Table 1: the heavy comparison sheet of the striped rice borer male and female pupa that gather in artificial feeding and rice field
| ? | Female pupa heavy (mg) | Male pupa heavy (mg) |
| The 1st generation of artificial feeding | 71.01 | 58.34 |
| The 2nd generation of artificial feeding | 79.47 | 59.04 |
| The 3rd generation of artificial feeding | 70.99 | 52.34 |
| The 4th generation of artificial feeding | 73.81 | 56.39 |
| The 5th generation of artificial feeding | 76.39 | 42.34 |
| The 6th generation of artificial feeding | 77.06 | 49.79 |
| The field population | 91.91 | 36.69 |
Result of the test shows: the striped rice borer of artificial feeding male pupa weight is higher than the field population, and female pupa weight then is lower than the field population.The result thinks that this rearing method can continue the large-scale breeding striped rice borer, and the pupa that obtains is heavy and the field population does not have notable difference.The striped rice borer pupa of gathering with artificial feeding striped rice borer and the field of embodiment 2-5 heavily compares, and result of the test is similar with embodiment 1.
Claims (8)
1. the feed formula of striped rice borer artificial feeding is characterized in that being made up of following substances in parts by weight: distilled water 600-900 part, agar 12-18 part, rice stem powder 8-16 part; Ripe soy meal 40-55 part, rice husk meal 28-45 part, casein 18-28 part, dusty yeast 4-9 part; Sucrose 8-16 part, vitamin C 1-4 part, 21 gold medals are tieed up his 0.5-3 part, vitamin E 0.5-3 part; Choline Chloride 0.1-1.2 part, sorbic acid 3-8 part, aureomycin 3-8 part.
2. the feed formula of striped rice borer artificial feeding as claimed in claim 1 is characterized in that: distilled water 700-800 part, agar 15-18 part, rice stem powder 10-15 part; Ripe soy meal 45-50 part, rice husk meal 30-40 part, casein 20-25 part, dusty yeast 5-8 part; Sucrose 10-15 part, Catergen-3 part, his 1-2 part of 21 gold medals dimension, vitamin e1-2 part; Choline Chloride 0.3-0.8 part, sorbic acid 4-6 part, aureomycin 2-5 part.
3. the feed formula of striped rice borer artificial feeding as claimed in claim 1 is characterized in that: distilled water 720-780 part, agar 16-17 part, rice stem powder 12-14 part; Ripe soy meal 46-48 part, rice husk meal 32-35 part, casein 22-24 part, dusty yeast 6-7 part; Sucrose 12-14 part, Catergen .5-2.8 part, 21 gold medals are tieed up his 1.2-1.8 part, vitamin e1 .5-1.8 part; Choline Chloride 0.5-0.7 part, sorbic acid 4.5-5 part, aureomycin 3-4 part.
4. the feed formula preparation method of striped rice borer artificial feeding as claimed in claim 1 is characterized in that may further comprise the steps:
1) feed formula is divided into A, B, three parts of C, the A part is made up of distillation distilled water 400-500 part, agar 12-18 part; The B part is made up of distilled water 100-200 part, rice stem powder 8-16 part, ripe soy meal 40-55 part, rice husk meal 28-45 part; B part is by his 0.5-3 part of distilled water 100-200 part, casein 18-28 part, dusty yeast 4-9 part, sucrose 8-16 part, vitamin C 1-4 part, 21 gold medals dimension, vitamin E 0.5-3 part, Choline Chloride 0.1-1.2 part, sorbic acid 3-8 part, aureomycin 3-8 part;
2) at first according to prescription weighing good various piece, then with the agar that weighs up and pour in the distilled water, heating and stirring in pot; Agar is dissolved fully, pour the B part of having mixed thoroughly then into, A, B component are fully mixed thoroughly in agitator; Treat to pour the C part into when its temperature drops to 50-58 ° of C, pour in the larva box after mixing thoroughly again, treat that it cools off naturally after; Cover lid places the refrigerator of 3-5 ° of C to preserve subsequent use.
5. the extensive method for breeding of striped rice borer artificial feeding as claimed in claim 1 is characterized in that may further comprise the steps:
1) making of man-made feeds and preservation: feed formula is divided into A, B, three parts of C, and the A part is made up of distillation distilled water 400-500 part, agar 12-18 part; The B part is made up of distilled water 100-200 part, rice stem powder 8-16 part, ripe soy meal 40-55 part, rice husk meal 28-45 part; B part is by his 0.5-3 part of distilled water 100-200 part, casein 18-28 part, dusty yeast 4-9 part, sucrose 8-16 part, vitamin C 1-4 part, 21 gold medals dimension, vitamin E 0.5-3 part, Choline Chloride 0.1-1.2 part, sorbic acid 3-8 part, aureomycin 3-8 part; At first according to prescription weighing good various piece, then with the agar that weighs up and pour in the distilled water, heating and stirring in pot; Agar is dissolved fully, pour the B part of having mixed thoroughly then into, A, B component are fully mixed thoroughly in agitator; Treat to pour the C part into when its temperature drops to 50-58 ° of C, pour in the larva raising box after mixing thoroughly again, treat that it cools off naturally after; Cover lid places 3-5 ℃ refrigerator to preserve subsequent use;
2) insert the striped rice borer pieces of an egg: the larva raising box that man-made feeds will be housed gets warm again after a cold spell to room temperature naturally; After the rice leaf that will have blackening pieces of an egg is cut; Be suspended on the man-made feeds, the larva of hatching can fall on feed and pierce to get food automatically, and general 1ml feed inserts the striped rice borer ovum of two blackening; Place 26-30 ℃ insectary then, and check incubation rate two days later in the pieces of an egg access;
3) pupa collection: in the time of 20 days, the high instar larvae of survival is transferred to the larva raising box that contains fresh feed at larval growth, generally calculate, and insert the circle of pupating on the top of larva raising box according to the worm amount of every box 180-220 head;
4) become the worm's ovum collection: generally at larval growth during by 30-35 days, the circle of will pupating takes out, and places the adult cage of laying eggs; Place in the cage that a basin is tillered or the paddy rice of jointing stage; Female worm can lay eggs automatically on the rice leaf, and adult mating cage places 25-29 ℃: 20-24 ℃ and the dark laying coop of 12-16 light: 12-8, and the cage of laying eggs is the cylinder mould of 0.5-1 * 0.5-1 * 1 meter size; Change rice seedling every day, promptly sustainable acquisition striped rice borer pieces of an egg;
Repeating an above 1-4 step is sustainable extensive raising striped rice borer.
6. the extensive method for breeding of striped rice borer artificial feeding as claimed in claim 5; It is characterized in that described larva raising box adopts cylindrical food crisper commonly used to process its diameter 10-15cm, high 5-7cm; Chiseling diameter on the lid is the 3-5cm opening; Be used for that humidity keeps in the box, and seal, prevent that larva from fleeing from the copper yarn.
7. the extensive method for breeding of striped rice borer artificial feeding as claimed in claim 5; It is characterized in that the described circle of pupating adopts the ordinary packing corrugated paper to process; Be cut into wide rectangular of 1.5-2.5cm; Be rolled into the paper circle on suitable the placed larva raising box top of size, and be fixed into circle with pin.
8. the extensive method for breeding of striped rice borer artificial feeding as claimed in claim 5; The storage temperature that it is characterized in that refrigerator in the step 1) is 3.5-4.5 ℃; Step 2) temperature of insectary is 27-29 ℃ in; The Wen Guang of laying coop is respectively 26-28 ℃ in the step 4): 21-22 ℃, Light To Dark Ratio is a 13-15 light: 11-9 is dark.
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| CN104920824A (en) * | 2015-05-18 | 2015-09-23 | 广州甘蔗糖业研究所 | Feed for raising chilo venosatus walkers and preparation method of feed |
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| US20180368444A1 (en) * | 2016-10-25 | 2018-12-27 | Guangdong Institute Of Applied Biological Resources | Artificial diet for ghost moth larvae and preparation method thereof |
| US10681921B2 (en) * | 2016-10-25 | 2020-06-16 | Guangdong Institute Of Applied Biological Resources | Artificial diet for ghost moth larvae and preparation method thereof |
| CN107167583A (en) * | 2017-07-25 | 2017-09-15 | 江西省农业科学院植物保护研究所 | Method for detecting drug resistance of chilo suppressalis |
| CN107167583B (en) * | 2017-07-25 | 2019-08-13 | 江西省农业科学院植物保护研究所 | Method for detecting drug resistance of chilo suppressalis |
| CN109548755A (en) * | 2018-12-10 | 2019-04-02 | 江西省农业科学院植物保护研究所 | A kind of method for breeding of striped rice borer |
| CN109548755B (en) * | 2018-12-10 | 2021-06-08 | 江西省农业科学院植物保护研究所 | Chilo suppressalis breeding method |
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| CN110604102A (en) * | 2019-11-05 | 2019-12-24 | 江苏省农业科学院 | Chilo suppressalis standardized feeding and management method |
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