CN101491307A - Rice leaf roller artificial feedstuff and preparation method and artificial feeding method applied on rice leaf roller - Google Patents
Rice leaf roller artificial feedstuff and preparation method and artificial feeding method applied on rice leaf roller Download PDFInfo
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- CN101491307A CN101491307A CNA2009100251808A CN200910025180A CN101491307A CN 101491307 A CN101491307 A CN 101491307A CN A2009100251808 A CNA2009100251808 A CN A2009100251808A CN 200910025180 A CN200910025180 A CN 200910025180A CN 101491307 A CN101491307 A CN 101491307A
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Abstract
The invention discloses a formulation of an artificial feed for cnaphalocrocis medinalis guenee, a method for preparing the same and a method for breeding the cnaphalocrocis medinalis guenee, which belongs to the technical field of insect feed and breeding. The formulation of the feed comprises the following components in portion by weight: 2 to 4 portions of casein, 0 to 4 portions of yeast powder, 1.5 to 3 portions of glucose, 0.5 to 2 portions of cane sugar, 2 to 5 portions of wheat-germ powder, 0.01 to 0.02 portion of vitamin B complex, 0.2 to 0.4 portion of vitamin C, 0.01 to 0.02 portion of vitamin E, 1 to 4 portions of agar, 0 to 2 portions of cellulose, 2 to 4 portions of rice leaf dry powder (which is prepared from rice leaves of tillering stage by drying and crushing at a temperature of 60 DEG C), 0.2 to 0.4 portion of 4M potassium hydroxide, 0.1 to 0.2 portion of sorbic acid, 0 to 0.2 portion of nipalgin, and 90 to 120 portions of water. The feed is prepared simply with low cost, and is favorable for the cnaphalocrocis medinalis guenee which has normal growth and development, thereby overcoming the difficulty that the cnaphalocrocis medinalis guenee cannot be bred for a successive generation artificially, achieving the artificial scale breeding of insects, and providing a material and a method for scientific experiments, chemical medicament screening and the production of a viral pesticide.
Description
Technical field
The present invention relates to insect feedstuff and breeding technology field, relate in particular to rice leaf folder man-made feeds preparation and be used for the rearing method that subculture is cultivated leaf-roller.
Background technology
Rice leaf folder (rice leaf roller), another name are scraped blue or green worm, leaf folder, white leaf worm etc., belong to Lepidoptera (Lepidopwera), and Pyralidae (Pyarlidae) is a kind of important migratory pest of China paddy rice of causing harm.The vertical leaf roll sheet of larva is got to eat and is caused harm, and only stays epidermis, forms white streak, influences the crop photosynthesis effect.In the paddy rice middle and later periods, harm top functional leaf, influence grouting causes that solid not full, empty flat rate increases, mass of 1000 kernel reduces, but general underproduction 2-3 become, heavy more than 5 one-tenths, rice field a slice is withered in vain when taking place greatly, even No kernels or seeds are gathered, as in a year of scarcity.This worm is serious day by day in China's generation since 2000, area takes place every year surpass 2,000 ten thousand hm, average annual grain loss 760,000 t.2007, be subjected to factor affecting such as worm source radix, climatic factor, planting conditions, big generation the in big portion rice district, China south, area 2,530 ten thousand hm have taken place, for rice leaf roller since nineteen ninety is taken place by the highest time of area.For carrying out rice leaf roller generation, harm and Study of Prevention Technology in a deep going way, fundamentally control the outburst harm of insect, demand setting up the rice leaf folder laboratory population urgently, artificial scale captive breeding insect, for the production of screening of scientific experimentation, chemical agent and viral insecticide provides material and method, therefore must solve man-made feeds prescription, the preparation of nursing rice leaf folder larva and use this man-made feeds whole years, the matching method of subculture raising leaf-roller.
The rice leaf roller paddy rice of mainly causing harm, barley, wheat, sugarcane, chestnut idol have harm, also can also get grassy weeds such as food barnyard grass, trip grass, lady's-grass, cogongrass, green bristlegrass, reed.The domestic resistance to the action of a drug to rice leaf roller is measured or directly introduce from the field mostly in the examination worm source that supplies of the test of pesticide effectiveness, raises from field transplanting rice seedlings to the indoor short-term of carrying out then, and is used for various tests.At present, the report of domestic still unmatchful rice leaf roller artificial feeding technology illustrates that the artificial feeding of this worm acquires a certain degree of difficulty.
Rice leaf roller man-made feeds raising aspect, the Teng Ji of Japan thinks can make the egg laying amount of rice leaf roller reduce with the man-made feeds raising, if egg laying amount can descend more under the short-day condition.Go up and the insect feedstuff of selling with Japan has been reported as basic feed in the field,, be equipped with the paddy rice of drying or the man-made feeds that the maize leaves powder is made, with the mass rearing and the method for breeding rice leaf roller again in the ratio of basic feed and leaf powder.But he finds, although added anticorrisive agent in feed, supports in the worm process Signs of Mould still takes place repeatedly.On the method for breeding of rice leaf roller larvae, a kind of is large glass room or guard, simulates the field environment within it and raises.Another kind of with less raisings such as container, main in order to cooperate the use of man-made feeds, the too small meeting of container causes polypide to diminish.On rice leaf roller laid eggs method, middle mountain, island etc. were put into polyethylene plastic bag with adult and are laid eggs, and used the method for scissors clip ovum attachment before the larva hatching.Gutian allows adult directly lay eggs on the rice leaf, directly gets the food rice seedlings after the larva hatching.Lei Yanyuan has compared 4 kinds of different methods and propose to adopt simple and easy carton method to lay eggs of laying eggs, but egg laying amount list female only 45, and it is loaded down with trivial details to adopt ovum, is difficult to standardized operation.
Summary of the invention
The objective of the invention is,, provide suitable man-made feeds of feeding rice leaf roller of a kind of energy and preparation method thereof at the deficiency of above-mentioned existence; Another object of the present invention be propose to use these man-made feeds carry out raising the whole year, subculture, to reach the method in year-round supply rice leaf roller standard worm source.
The object of the invention is achieved through the following technical solutions:
A kind of man-made feeds of feeding rice leaf roller, the weight portion of this man-made feeds prescription and each component is: casein 2-4 part, dusty yeast 0-4 part, glucose 1.5-3 part, sucrose 0.5-2 part, wheat germ powder 2-5 part, vitamin B compound 0.01-0.02 part, vitamin C 0.2-0.4 part, vitamin E 0.01-0.02 part, agar 1-4 part, cellulose 0-2 part, paddy rice leaf dried powder (60 ℃ of oven dry of rice plant of tillering stage blade are pulverized and made) 2-4 part, 4M potassium hydroxide 0.2-0.4 part, sorbic acid 0.1-0.2 part, nipalgin 0-0.2 part, water 90-120 part.
The preparation method of above-mentioned rice leaf roller man-made feeds carries out according to the following steps:
(1) with each component according to the prescription weighing, standby;
(2) casein, 4M KOH and low amounts of water are heated to 90 ℃, make casein dissolving after, add dusty yeast, stir and make its dissolving.
(3) the paddy rice leaf dried powder is flooded 30min in suitable quantity of water after, 80 ℃ of water-bath 10min add agar and supplementing water to total formula ratio, heating makes the agar dissolving.
(4) solution with (2) and (3) mixes, and after adding wheat germ powder, cellulose, glucose, sucrose successively, fully mixes.
When (5) solution to be mixed (4) temperature is reduced to 50-60 ℃, add vitamin B compound, vitamin C, vitamin E, sorbic acid and nipalgin successively, after stirring, pour into to be cooled in the container after the sterilization and condense, put 4-5 ℃ of fresh-keeping storage the man-made feeds product.
Using artificial feed whole year, subculture are raised the method for rice leaf folder, carry out according to following steps:
(1) cultivates the green corn seedling: will become cooked maize seed seed soaking 24h, coated with wet cloth vernalization.The corn seed that will show money or valuables one carries unintentionally places the germination box interior in 25 ℃ of illumination box germinating growths, gets required green corn seedling after 5-8 days.
(2) laying eggs of rice leaf roller: be the utensil of laying eggs with commercially available 100-125ml disposal plastic cup,, fill in the cotton balls that dips in the 5-10% honey in the hole that a diameter 5-8mm is opened in the plastic cup bottom.Insert 3 pairs of rice leaf roller adults that just sprouted wings in the cup, seal, place in 23 ℃ of biochemical incubators and lay eggs with preservative film.
(3) treat egg development to the blackhead phase, in cup, put into 1-2 and open the maize seedling blade that the larva that just incubates is automatically transferred on the maize seedling blade and gets food.Have the maize leaf of larva to move in the plastic culture dish transfer, the moisture releasing cotton balls is preserved moisture in the ware, places 25 ℃, the biochemical incubator of relative humidity 95% to raise, and changes the fresh corn blade at interval in 2-3 days.
(4) be folded into the corrugation of wide 2cm, high 0.5cm with glassine paper, place the plastic culture dish of sterilization back diameter 9-11cm, cutting the thick man-made feeds thin slice of 2-4mm places on the corrugation, to insert in the culture dish with the larva that the maize seedling blade was raised 5-6 days, every ware connects worm about 50, be covered with antiseptic hygiene paper after, cover completely with ware, place 25 ℃, the biochemical incubator of relative humidity 95% to raise, changed a feed at interval in 6-7 days.
(5) larva is after 3 ages, and every ware stocking density remains on the 20-30 head, and mature larva is cocoond in feed bottom or toilet paper and pupated.
(6) continue after pupa is sprouted wings by above-mentioned steps (1), (2), (3), the continuous generation raising of (4) and (5) cycling.
Advantage of the present invention is:
(1) this artificial feedstuff source is sufficient, and manufacture craft is simple, and cost is low, and 1 yuan of every 100g feed cost can be raised 1000 above rice leaf folder larvas.
(2) this artificial feed good palatability, rice leaf roller larvae happiness food, it is normal to grow, and through the raising of going down to posterity of continuous 3 generations, larva is on average gone through phase 24.3d, survival rate 73.8%, pupa is gone through phase 6.9d, survival rate 94.7%, 87.3 of egg laying amounts/female, incubation rate 90.1%.
(3) adopt the method for breeding of the artificial feed of the present invention, overcome the high shortcoming of the newly hatched larvae death rate, be built into the knot bud by glassine paper corrugation and the simulation of man-made feeds thin slice simultaneously, get food, sheltered situation for larva provides in conjunction with the maize seedling tender leaf.
(4) adopt the method that cup is laid eggs of moulding in the method for breeding of the present invention, the cotton balls that dips in honey keeps humidity in the cup again both for the rice leaf roller adult provides nutrition, has improved egg laying amount, and female the laying eggs of the highest list answered 117.6.
The specific embodiment
Embodiment 1:(rice leaf roller man-made feeds preparation 1)
Each amounts of components of this example is: casein 3.75g, wheat germ powder 2.5g, sorbic acid 0.2g, sucrose 1.25g, glucose 2.5g, agar 1.5g, 4M KOH 0.3g, vitamin C 0.4g, vitamin E 0.02g, vitamin B compound 0.02g, water 100g, rice leaf dried powder 3g.Be mixed with the rice leaf folder man-made feeds according to following steps:
(1) casein, 4M KOH and 30ml water are heated to 90 ℃, make the casein dissolving.
(2) the paddy rice leaf dried powder is flooded 30min in 30ml water after, 80 ℃ of water-bath 10min add agar and supplementing water 2.5ml, heating makes the agar dissolving.
(3) solution with (1) and (2) mixes, and after adding wheat germ powder, glucose, sucrose successively, fully mixes.
When (4) solution to be mixed (3) temperature is reduced to 50-60 ℃, add vitamin B compound, vitamin C, vitamin E, sorbic acid successively, after stirring, pour into to be cooled in the container after the sterilization and solidify, put 4-5 ℃ of fresh-keeping storage the man-made feeds product.
Embodiment 2:(rice leaf roller man-made feeds preparation 2)
Each amounts of components of this example is: casein 2g, dusty yeast 2g, wheat germ powder 2.5g, sorbic acid 0.16g, nipalgin 0.16g, sucrose 1g, glucose 2g, agar 2g, cellulose 1g, 4MKOH 0.3g, vitamin C 0.4g, vitamin E 0.02g, vitamin B compound 0.02g, water 100g, paddy rice leaf dried powder 2.5g.Step according to embodiment 1 is mixed with the rice leaf folder man-made feeds.
The artificial feeding of embodiment 3:(rice leaf roller larvae)
Man-made feeds with embodiment 1, embodiment 2 preparations are fed rice leaf roller larvae in conjunction with the maize seedling blade, raise separately with the maize seedling blade simultaneously to compare.Specifically carry out according to the following steps:
(1) cultivation of green corn seedling: will become cooked maize seed seed soaking 24h, coated with wet cloth vernalization.The corn seed that will show money or valuables one carries unintentionally places the germination box interior in 25 ℃ of illumination box germinating growths, gets required green corn seedling after 5-8 days.
(2) transfer there is the maize leaf of just incubating rice leaf roller larvae move in the diameter 9cm plastic culture dish, add the fresh corn seedling leaf, the moisture releasing cotton balls is preserved moisture in the ware, place 25 ℃, the biochemical incubator of relative humidity 95% to raise, every ware connects 100 of worms, changes the fresh corn blade at interval in 2-3 days.
(3) be folded into the corrugation of wide 2cm, high 0.5cm with glassine paper, place the plastic culture dish of sterilization back diameter 9cm, cutting the preparation 1 of the thick man-made feeds of 2mm and man-made feeds respectively prepares on the corrugation that 2 thin slices place different culture dishes, to insert in the different disposal with the larva that the maize seedling blade was raised 6 days, every ware connects 50 of worms, be covered with antiseptic hygiene paper after, cover completely with ware, place 25 ℃, relative humidity 95% biochemical incubator to raise, changed a feed in 6-7 days at interval, until all pupating.
(5) the independent processing of raising of maize seedling is made feed with the maize seedling blade always, changes fresh blade in 2-3 days at interval, and every ware stocking density is controlled in 20 after 2 ages, and the built-in wet cotton ball of ware is preserved moisture, until pupate comprehensively.
The raising result such as the following table of DIFFERENT FEED:
Claims (3)
1, a kind of man-made feeds of feeding rice leaf roller is characterized in that the weight portion of this man-made feeds prescription and each component is: casein 2-4 part, dusty yeast 0-4 part, glucose 1.5-3 part, sucrose 0.5-2 part, wheat germ powder 2-5 part, vitamin B compound 0.01-0.02 part, vitamin C 0.2-0.4 part, vitamin E 0.01-0.02 part, agar 1-4 part, cellulose 0-2 part, paddy rice leaf dried powder 2-4 part, 4M potassium hydroxide 0.2-0.4 part, sorbic acid 0.1-0.2 part, nipalgin 0-0.2 part, water 90-120 part.
2, the method for preparing claim 1 man-made feeds is characterized in that carrying out according to the following steps:
(1) with each component according to the prescription weighing, standby;
(2) casein, 4M KOH and low amounts of water are heated to 90 ℃, make casein dissolving after, add dusty yeast, stir and make its dissolving;
(3) the paddy rice leaf dried powder is flooded 30min in suitable quantity of water after, 80 ℃ of water-bath 10min add agar and supplementing water to total formula ratio, heating makes the agar dissolving;
(4) solution with (2) and (3) mixes, and after adding wheat germ powder, cellulose, glucose, sucrose successively, fully mixes;
When (5) solution to be mixed (4) temperature is reduced to 50-60 ℃, add vitamin B compound, vitamin C, vitamin E, sorbic acid and nipalgin successively, after stirring, pour into to be cooled in the container after the sterilization and solidify, put 4-5 ℃ of fresh-keeping storage the man-made feeds product.
3, application rights requires the man-made feeds whole year of 1,2 preparations, the method that subculture is raised rice leaf folder, it is characterized in that carrying out according to following steps:
(1) cultivate the green corn seedling: will become cooked maize seed seed soaking 24h, coated with wet cloth vernalization, the corn seed that will show money or valuables one carries unintentionally places the germination box interior in 25 ℃ of illumination box germinating growths, gets required green corn seedling after 5-8 days;
(2) laying eggs of rice leaf roller: be the utensil of laying eggs with commercially available 125ml disposal plastic cup, the hole of opening a diameter 5-8mm in the plastic cup bottom, fill in the cotton balls that dips in the 5-10% honey, insert 3 pairs of rice leaf roller adults that just sprouted wings in the cup, seal with preservative film, place in the 23-25 ℃ of biochemical incubator and lay eggs;
(3) treat that egg development is to the blackhead phase, in cup, put into 1-2 and open the maize seedling blade, the larva that just incubates is automatically transferred on the maize seedling blade and gets food, there is the maize leaf of larva to move in the plastic culture dish transfer, the moisture releasing cotton balls is preserved moisture in the ware, place 25 ℃, the biochemical incubator of relative humidity 95% to raise, changed the fresh corn blade at interval in 2-3 days;
(4) be folded into the corrugation of wide 2cm, high 0.5cm with glassine paper, place the plastic culture dish of sterilization back diameter 9-11cm, cutting the thick man-made feeds thin slice of 2-4mm places on the corrugation, to insert in the culture dish with the larva that the maize seedling blade was raised 5-6 days, every ware connects worm about 50, be covered with antiseptic hygiene paper after, cover completely with ware, place 25 ℃, the biochemical incubator of relative humidity 95% to raise, changed a feed at interval in 6-7 days;
(5) larva is after 3 ages, and every ware stocking density remains on the 20-30 head, and mature larva is cocoond in feed bottom or toilet paper and pupated;
(6) continue after pupa is sprouted wings by above-mentioned steps (1), (2), (3), the continuous generation raising of (4) and (5) cycling.
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