CN108813114A - Corn borer larvae man-made feeds and its preparation method and application - Google Patents
Corn borer larvae man-made feeds and its preparation method and application Download PDFInfo
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- CN108813114A CN108813114A CN201810667004.3A CN201810667004A CN108813114A CN 108813114 A CN108813114 A CN 108813114A CN 201810667004 A CN201810667004 A CN 201810667004A CN 108813114 A CN108813114 A CN 108813114A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Abstract
The invention discloses a kind of corn borer larvae man-made feeds and its preparation method and application, which is indicated with mass fraction, by following component component：Raw 100~200 parts of wheat germ, 100~200 parts of soy meal, 25~35 parts of milk powder, 80~90 parts of yeast powder, 70~80 parts of white sugar, 7~8 parts of multi-vitamins, 20~30 parts of agar, 4~6 parts of methylparaben, 4~6 parts of sorbic acid, 6~8 parts of ascorbic acid, 30~40% 2~3 parts of formalins, 1500~2000 parts of water.The present invention devises the preparation method of the feed, additionally provides the method that the feed carries out raising corn borer.Corn borer man-made feeds material is easy to get, nutritional ingredient is comprehensive, operating process is simple, time cost is low, survival rate of larvae is high, percentage of pupation is high, pupa head is big, eclosion rate is high, egg laying amount is big, feeding effect is stable, be conducive to raise corn borer larvae on a large scale, there is very strong operability and practicability.
Description
Technical field
The present invention relates to corn borer breeding technology fields, and in particular to a kind of corn borer larvae man-made feeds and its preparation side
Method and application.
Background technique
Corn borer, also known as corn stalk borer are subordinate to Lepidoptera (Lepidoptera), Pyralidae (Pyralidae), distribution
It is the Severe pests on corn in China various regions.The lobus cardiacus of corn borer main harm corn, female fringe, filigree, the positions such as stalk.
Corn borer endangers the lobus cardiacus of corn in larva period, and there are many semitransparent thin films of irregular alignment for the maize leaves meeting after being gnawed
The hole of shape, will lead to lobus cardiacus can not grow.Until corn starts booting, corn borer meeting feeding fringe portion seriously affects corncob
Growth and development.It reeling off raw silk from cocoons grouting period in corn, corn borer meeting feeding filigree causes corn that can not be in the milk, after corn heading, corn
Snout moth's larva can seriously affect the yield of corn crop in the inside and outside feeding corncob tissue of corncob and fruit particle.In recent years,
The occurrence degree cumulative year after year of corn borer has seriously affected the normal production of corn, has at least caused 10% corn to subtract every year
It produces, the even underproduction is up to 30% when serious.
Indoors artificial raising corn borer can provide a large amount of worm ages and the consistent mark of nutrition condition for scientific experimentation research
Quasi- test worm, the basis that the breeding etc. to carry out biological characteristics, insecticide and Bt protein screening, parasitic wasp is studied provide guarantor
Barrier.
Currently, matching for Ostrinia furnacalis man-made feeds can be divided into two major classes, it is the object for condensing gelation that one kind, which is with agar,
Matter is as carrier, and the feed worked it out is able to satisfy the psychological need of borer pest feeding, and most common formula is Chinese agriculture
New No. 7 man-made feeds of the researchers such as academy of sciences Plant Protection Institute Zhou great Rong exploitation（Zhou great Rong etc., 1980）, but with
The raising generation increases, and different degrees of degradation phenomena occurs in the corn borer population of raising, and the most outstanding is pupa weight
It reduces and the egg laying amount of adult reduces.On this basis, 103110020 A of Chinese patent literature CN discloses a kind of corn borer
Man-made feeds, being mainly characterized by of the formula are added to medicinal herb components in above-mentioned formula, although which makes corn borer
Pupa weight all increase with egg laying amount, however the medicinal herb components are expensive, and cost is high, and preparation process elaborate, hardly possible
To promote.
In addition a kind of formula is the substitute for finding agar, on the basis of new No. 7 man-made feeds, Chinese patent literature CN
1160494 A disclose the substance with JSMD（Sawdust）Replace agar, but Yunnan Prov Agriculture University is matched with this formula and production method
When the Ostrinia furnacalis on the ground such as Kunming, Xun Dian, Wuding County is picked up from the feed raising of system, the effect is unsatisfactory, it is believed that may be used
The ingredient of sawdust is not suitable for the feed, and then, on the basis of new No. 7 formulas, developing one kind with cornstalk powder is
The feed formula of primary raw material（With reference to 102669472 A of Chinese patent literature CN）.However, using corn stover as primary raw material
Formula the problem of being not easy there is materials；In addition, once transgenic insect-resistant corn after China Businessization plantation, safe jade
Rice stalk powder source may be unable to get guarantee.
It would therefore be highly desirable to develop a kind of material requested on the market simple easily purchase, standardization level is high, manufacturing process is simple,
Time cost is saved, while every lifes such as high survival rate, percentage of pupation are high, pupa head is big, eclosion rate is high, egg laying amount is more can be raised out
The reason corn borer larvae man-made feeds formula that index is all fairly good and feeding effect is stable.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of corn borer larvae man-made feeds and its preparation method and application,
Aim to solve the problem that current artificial corn borer feed formula raw material be not easy to obtain, complex manufacturing process, it is time-consuming and laborious and caused
Continuously rearing is unstable, percentage of pupation and eclosion rate are low, the technical problem of adult consistency difference.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
The present invention devises a kind of corn borer larvae man-made feeds, is indicated with mass fraction, by following component component：Raw wheat germ
100~200 parts, 100~200 parts of soy meal, 25~35 parts of milk powder, 80~90 parts of yeast powder, 70~80 parts of white sugar, compound dimension life
7~8 parts of element, 20~30 parts of agar, 4~6 parts of methylparaben, 4~6 parts of sorbic acid, 6~8 parts of ascorbic acid, 30~40%（Body
Product percentage）2~3 parts of formalin, 1500~2000 parts of water.
Preferably, the multi-vitamins are Lepidoptera multi-vitamins.
The present invention devises the preparation method of corn borer larvae man-made feeds described in one kind, includes the following steps：
（1）The soy meal, yeast powder are sterilized；
（2）Water is heated, the raw wheat germ, soy meal, milk powder, yeast powder, white sugar is added, stirs evenly, obtains component A；
（3）The methylparaben and sorbic acid are added into the agar, side is added waterside stirring, mixture is obtained, by institute
It obtains mixture and is heated to component all dissolutions while stirring, obtain component B；
（4）To step（3）Temperature is cooling, and component A is added in component B and is stirred evenly, the ascorbic acid, compound dimension is added
Raw element and formaldehyde continue to stir evenly,.
Preferably, in the step（2）In, the temperature control of the heating is 40~50 DEG C.
Preferably, in the step（4）In, the temperature control of the cooling is 50~60 DEG C.
The present invention provides a kind of methods for carrying out raising corn borer using the corn borer larvae man-made feeds, including
Following steps：
（1）When raising newly hatched larvae, the access of squab corn borer larvae is equipped with the corn borer larvae man-made feeds
Feeding worm plate in, be placed in that temperature is 25~27 DEG C, the photoperiod is（14~16）L：（8~10）D, relative humidity is 70~80%
Under the conditions of pupate to it；
When breeding grub, every 3~4 days corn borer larvae man-made feeds described to larva feeding, being placed in temperature is 25~27
DEG C, photoperiod 16L：8D, humidity are pupated under conditions of being 70~80% to it；
（2）Epidermis is become auburn pupa to choose, be placed in temperature be 25~27 DEG C, photoperiod 16L：8D, humidity are 70~80%
Under conditions of to its emergence；
（3）After adult eclosion, the gender of emerging adult is recorded, according to male and female 1：1 proportional paired, being placed in temperature is 25~27
DEG C, photoperiod 16L：8D, humidity are laid eggs under conditions of being 70~80% to it；
（4）It collects pieces of an egg to be placed in the valve bag of moisturizing, to egg hatching；
（5）After larva hatches, repeating said steps（1）~（4）, circulation goes down, and completes continuously rearing.
Compared with prior art, the beneficial technical effect of the present invention lies in：
1. corn borer man-made feeds of the present invention do not need addition medicinal herb components, rinsing is eliminated, is boiled, filtered, sterilizing waited
Journey reduces operating procedure, keeps preparation method simpler, time saving and energy saving.
2. corn borer larvae man-made feeds of the present invention contain raw wheat germ and milk powder, raw wheat germ contains a large amount of high-quality protein
Matter, crude fibre, several mineral materials, there are also the nutritional ingredients such as multivitamin；Contain protein, fat, carbon hydrate in milk powder
A variety of nutriments such as object, calcium, iron, zinc, linoleic acid meet corn borer low instar larvae so that the ingredient of feed is more comprehensive
The requirement for needing a large amount of protein is more advantageous to larva uniform growth, improves percentage of pupation, 5 instar larvaes of raising are loose, pupa weight
It improves, Adult worms producting eggs amount is big.
It is to purchase in the market 3. raw material of the present invention are easy to get, standardization level is high, even if without professional technique background
Personnel can grasp production method after simple training.
4. the invention avoids because of JMSD（Sawdust）Feeding effect does not have the standard of the ingredients such as generality and cornstalk powder
The problems such as standby manufacturing process is cumbersome, also for the commercialization of transgenic corns in future after, not can guarantee based on cornstalk powder
The safety issue of man-made feeds raising corn borer provides risk averse technology.
5. the corn borer larvae growth and development that the present invention raises is neat, consistency is good, continuously rearing effect stability, for science
Research, biological control（Such as insecticide and Bt protein screening）And production（Such as the breeding of parasitic wasp）Need etc. to provide substance guarantor
Barrier.
6. corn borer larvae man-made feeds material of the invention is easy to get, nutrition is comprehensive, and the corn borer larvae raised is pupated
Rate is high, and pupa head is big, and eclosion rate is high, single female egg laying amount it is high, it can be achieved that indoor man-made feeds continuously rearing, and then reduce and draw
Kind cost.
Detailed description of the invention
Fig. 1 is the larva photo of feeding corn borer larvae man-made feeds of the present invention；
The photo that Fig. 2 is pupated for the larva that corn borer larvae man-made feeds of the present invention are raised.
Specific embodiment
Illustrate a specific embodiment of the invention with reference to the accompanying drawings and examples, but following embodiment is used only in detail
It describes the bright present invention in detail, does not limit the scope of the invention in any way.
Related instrument and equipment is routine instrument device unless otherwise instructed in the examples below；It is related
Reagent is commercially available conventional reagent unless otherwise instructed；Related test method is unless otherwise instructed conventional method.
Embodiment one：A kind of corn borer larvae man-made feeds
1. corn borer larvae man-made feeds, are indicated with mass fraction, following components are contained in feed formula：Raw 150 parts of wheat germ,
150 parts of soy meal, 30 parts of milk powder, 85 parts of yeast powder, 75 parts of white sugar, 7.5 parts of multi-vitamins, 25 parts of agar, methylparaben 5
Part, 5 parts of sorbic acid, 7 parts of ascorbic acid, 30~40% 2.17 parts of formalins, 1700 parts of water.
Wherein, multi-vitamins are Lepidoptera multi-vitamins（Vitamin Mix Lepidoptera, by Frontier
The production of Agricultural Science company）.
2. the preparation of the man-made feeds of corn borer larvae
Component A raw material：Raw wheat germ powder 150g, soy meal 150g, milk powder 30g, yeast powder 85g, white sugar 75g, distilled water 900ml；
Component B raw material：Agar 25g, methylparaben 5g, distilled water 600ml；
Component C raw material：Ascorbic acid 7g, multi-vitamins 7.5g, 40% formaldehyde 2ml.
The man-made feeds preparation process of corn borer larvae includes the following steps：
（1）Soy meal, yeast powder are sterilized 30 minutes at 120 DEG C；
（2）Weigh component A each component according to mass fraction, heat water to 40~50 DEG C, be then added raw wheat germ, soy meal,
Yeast powder, milk powder, white sugar, stir evenly, and obtain component A；
（3）Methylparaben and sorbic acid are added into agar, side is added waterside and is stirred, obtains mixture, gained is mixed
It closes object and is heated to component all dissolutions while stirring, obtain component B；
（4）To step（3）When temperature is cooled to 50~60 DEG C, component A is added in component B and is stirred evenly, is eventually adding anti-
Bad hematic acid, multi-vitamins and formaldehyde continue to stir evenly.
3. the method for the man-made feeds raising corn borer using corn borer larvae：
（1）The careful access of squab corn borer larvae is equipped in the feeding worm plate of man-made feeds, a hole by newly hatched larvae raising
It one, closes the lid, being placed in temperature is 25~27 DEG C, and the photoperiod is（14~16）L：（8~10）D, relative humidity are 70~80%
Under conditions of, it pupates to it, turn into adult；
（2）Larva raising replaces feed to larva, or replaces feed as the case may be, is placed in temperature for every 3~4 days during raising
Degree is 25~27 DEG C, photoperiod 16L：8D, humidity are pupated to raise in 70~80% incubator；
（3）Epidermis is become auburn pupa and chosen, is placed in test tube or plate by the collection of pupa, and one, a hole pupa, the inside is put one and is stained with
Wet cotton balls provides the emergence that humidity facilitates pupa, and temperature is 25~27 DEG C, photoperiod 16L：8D, humidity are 70~80%
In incubator；
（4）Adult eclosion records the gender of each emerging adult, according to male and female 1：The every 30 first groups of pairings of 1 ratio allow it to produce
Ovum mixes female male imago, and the sucrose water or hydromel of 8~10% concentration is added, and provides nutrition point, insect box surrounding for adult
Paraffin paper is posted, provides place for corn borer oviposition, being placed in temperature is 25~27 DEG C, photoperiod 16L：8D, humidity are 70~80%
Incubator in；
（5）The collection of ovum, the paraffin paper after collecting oviposition, can be cut into small pieces, pieces of an egg are placed in the valve bag of sealing, filter paper
Being placed in valve bag makes its moisturizing, and being subsequently placed in temperature is 25~27 DEG C, photoperiod 16L：8D, the training that humidity is 70~80%
It supports in case, facilitates the hatching of ovum.
（6）Monoculture after larva hatches, repeats step（1）~（5）, circulation goes down, and completes continuously rearing；
（7）The feed cut is placed in insect box by group rearing, and cassette bottom spreads one layer of filter paper both moisturizing or the moisture absorption, and blackhead pieces of an egg are put
On feed, larva go out worm can direct feeding, pupate, repeat step（4）,（5）.
Embodiment two：A kind of corn borer larvae man-made feeds
With embodiment one the difference is that：
Corn borer larvae man-made feeds, are indicated with mass fraction, and following components are contained in feed formula：Raw 100 parts of wheat germ, soybean
200 parts of powder, 25 parts of milk powder, 80 parts of yeast powder, 80 parts of white sugar, 7 parts of multi-vitamins, 20 parts of agar, 4 parts of methylparaben, mountain
6 parts of pears acid, 8 parts of ascorbic acid, 30~40% 2 parts of formalins, 1500 parts of water.
Embodiment three：A kind of corn borer larvae man-made feeds
With embodiment one the difference is that：
Corn borer larvae man-made feeds, are indicated with mass fraction, and following components are contained in feed formula：Raw 200 parts of wheat germ, soybean
100 parts of powder, 35 parts of milk powder, 90 parts of yeast powder, 70 parts of white sugar, 8 parts of multi-vitamins, 30 parts of agar, 6 parts of methylparaben, mountain
4 parts of pears acid, 6 parts of ascorbic acid, 30~40% 3 parts of formalins, 2000 parts of water.
Example IV：Corn borer larvae man-made feeds are detected to the development index of corn borer larvae and the shadow of adult reproductive capacity
It rings
1. selected insect source
Corn borer is the population in the acquisition of Henan Xinxiang Yuanyang area in 2015, is risen indoors with conventional manual's feed captive breeding
The population come.
2. test grouping
Two kinds of feeds raising that man-made feeds of the invention are recorded with new No. 7 formulas and 103110020 A of patent document CN
The development index and adult reproductive capacity of larva are compared.
3. test method
The corn borer man-made feeds of embodiment one are cut into small pieces, 24 holes is put into and supports in worm plate, the corn borer larvae just incubated is connect
Enter to 24 holes and support in worm plate, one, a hole closes the lid, and each processing there are 96 larvas, and it is 26 that larva, which is then placed in temperature,
± 0.5oC, photoperiod 16L：8D, humidity is in 70~80% incubator.In triplicate.
Larvae alive is recorded, is pupated the time, pupa weight, emergence time, by each processing according to male and female 1 are claimed：1 ratio is put into
In insect box, stencil paper is around sticked, every 30 first groups allow it to lay eggs.The absorbent cotton of 10% sucrose water or hydromel will be soaked with
It is put into culture dish, culture dish is put into insect box, provide nutrition for adult.The pieces of an egg on primary oviposition paper are collected within every 2 days,
Pieces of an egg quantity is recorded, is then placed in pieces of an egg in the valve bag of sealing.3 generations of continuous raising, statistics survival rate of larvae, larva are gone through
The indexs such as phase, percentage of pupation, pupa time, pupa weight, eclosion rate, male and female Life of Adult, single female eggs on average amount.
Variance analysis and significance of difference test are carried out using SPSS20.0 statistical software.It, will be continuous for the ease of comparing
The three generations of raising is averaged, and then statisticallys analyze.
4. the influence of the development index of pair corn borer larvae
The results are shown in Table 1：
The corn borer larvae development index parameter of several formula raisings of table 1
。
It (indicates：Data are mean value ± SE in table 1, and going together, interior letter is different to indicate significant difference,P=0.05, similarly hereinafter)
As can be seen from the table, the female pupa weight of Three feed formula raising, male pupa weight, there were significant differences for egg laying amount（Female pupaF 2,8=13.801,P=0.006；Male pupaF 2,8=8.854,P=0.034；Egg laying amountF 2,8=197.269,P=0.000）.Embodiment one
The male and female pupa weight of feed raising and single female egg laying amount are all remarkably higher than new No. 7 feed formulas, with patent document CN 103110020
A record formula raising without significant difference.Illustrate, feed formula of the present invention is not although add medicinal herb components, raising effect
Fruit significantly improves, and has still achieved the effect that the formula raising for adding medicinal herb components.Feed of the invention both ensure that raising effect
Fruit, and greatly reduce and be produced into book, simplify preparation process.
5. the influence pair adult reproductive capacity
Observation and the record that the other indexs of larva of feed formula of the present invention raising have also been carried out with continuous three generations, as a result such as table 2
It is shown：
The other development index parameters of corn borer larvae of this formula raising of table 2
。
As shown in Table 2, larva averagely goes through the phase in 24d or so, and percentage of pupation is 85~86% or so, pupa time 9d or so, eclosion rate
96% or so, female moth service life 13d, male moth service life 12d, without significant difference between three generations（P> 0.05）, continuously rearing indoors
Effect stability, there is not degradation phenomena.
The larva cultivated is as shown in Figure 1, corn borer larvae is delithted with the feed, the remaining nothing that the feed being put into is eaten
Several, corn borer larvae is loose and healthy.
As shown in Fig. 2, corn borer pupa head is big after the larvae pupation cultivated, pupa weight is high, and uniformity is good, and is actively good for
Health.
The present invention is described in detail above in conjunction with drawings and examples, still, those of skill in the art
Member is it is understood that without departing from the purpose of the present invention, can also carry out each design parameter in above-described embodiment
Change, forms multiple specific embodiments, is common variation range of the invention, is no longer described in detail one by one herein.
Claims (6)
1. a kind of corn borer larvae man-made feeds, which is characterized in that indicated with mass fraction, by following component component：Raw wheat germ
100~200 parts, 100~200 parts of soy meal, 25~35 parts of milk powder, 80~90 parts of yeast powder, 70~80 parts of white sugar, compound dimension life
7~8 parts of element, 20~30 parts of agar, 4~6 parts of methylparaben, 4~6 parts of sorbic acid, 6~8 parts of ascorbic acid, 30~40% first
2~3 parts of aldehyde aqueous solution, 1500~2000 parts of water.
2. corn borer larvae man-made feeds according to claim 1, which is characterized in that the multi-vitamins are Lepidoptera
Multi-vitamins.
3. a kind of preparation method of corn borer larvae man-made feeds described in claim 1, which is characterized in that including following step
Suddenly：
（1）The soy meal, yeast powder are sterilized；
（2）Water is heated, the raw wheat germ, soy meal, milk powder, yeast powder, white sugar is added, stirs evenly, obtains component A；
（3）The methylparaben and sorbic acid are added into the agar, side is added waterside stirring, mixture is obtained, by institute
It obtains mixture and is heated to component all dissolutions while stirring, obtain component B；
（4）To step（3）Temperature is cooling, and component A is added in component B and is stirred evenly, the ascorbic acid, compound dimension is added
Raw element and formaldehyde continue to stir evenly,.
4. the preparation method of corn borer larvae man-made feeds according to claim 3, which is characterized in that in the step
（2）In, the temperature control of the heating is 40~50 DEG C.
5. the preparation method of corn borer larvae man-made feeds according to claim 3, which is characterized in that in the step
（4）In, the temperature control of the cooling is 50~60 DEG C.
6. a kind of method for carrying out raising corn borer using corn borer larvae man-made feeds described in claim 1, feature exist
In including the following steps：
（1）When raising newly hatched larvae, the access of squab corn borer larvae is equipped with corn borer larvae described in claim 1
In the feeding worm plate of man-made feeds, it is placed in that temperature is 25~27 DEG C, the photoperiod is（14~16）L：（8~10）D, relative humidity is 70
It pupates under conditions of~80% to it；
When breeding grub, corn borer larvae man-made feeds described in claim 1 are fed to larva within every 3~4 days, be placed in temperature
For 25~27 DEG C, photoperiod 16L：8D, humidity are pupated under conditions of being 70~80% to it；
（2）Epidermis is become auburn pupa to choose, be placed in temperature be 25~27 DEG C, photoperiod 16L：8D, humidity are 70~80%
Under conditions of to its emergence；
（3）After adult eclosion, the gender of emerging adult is recorded, according to male and female 1：1 proportional paired, being placed in temperature is 25~27
DEG C, photoperiod 16L：8D, humidity are laid eggs under conditions of being 70~80% to it；
（4）Collect pieces of an egg be placed in the valve bag of moisturizing, be placed in temperature be 25~27 DEG C, photoperiod 16L：8D, humidity 70
To egg hatching under conditions of~80%；
（5）After larva hatches, repeating said steps（1）~（4）, circulation goes down, and completes continuously rearing.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109965088A (en) * | 2019-03-01 | 2019-07-05 | 河南省农业科学院植物保护研究所 | Spodopterafrugiperda larva artificial feed, preparation method and application |
| CN114391516A (en) * | 2022-02-18 | 2022-04-26 | 吉林省农业科学院 | Medium for artificial inoculation of Asiatic corn borers in field and preparation method and application thereof |
| CN116530621A (en) * | 2023-06-14 | 2023-08-04 | 烟台大学 | Mythimna separata feed and indoor mythimna separata feeding method |
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| CN116530621A (en) * | 2023-06-14 | 2023-08-04 | 烟台大学 | Mythimna separata feed and indoor mythimna separata feeding method |
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