CN108208479B - Pure artificial feed suitable for feeding and experimental analysis of tea geometrid larvae - Google Patents
Pure artificial feed suitable for feeding and experimental analysis of tea geometrid larvae Download PDFInfo
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- 241001046947 Ectropis obliqua Species 0.000 title claims abstract description 59
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 claims abstract description 29
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- 229940030275 epigallocatechin gallate Drugs 0.000 claims abstract description 29
- 239000000843 powder Substances 0.000 claims abstract description 28
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- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 10
- 230000001488 breeding effect Effects 0.000 claims description 10
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 10
- 229960003178 choline chloride Drugs 0.000 claims description 10
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 10
- 150000003839 salts Chemical class 0.000 claims description 10
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 10
- 235000019764 Soybean Meal Nutrition 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
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- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
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- 150000001875 compounds Chemical class 0.000 claims description 2
- 230000027870 phototropism Effects 0.000 claims description 2
- 244000052616 bacterial pathogen Species 0.000 claims 1
- 241001122767 Theaceae Species 0.000 abstract description 17
- 230000019617 pupation Effects 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 10
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 14
- 235000010419 agar Nutrition 0.000 description 8
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 7
- 229960001948 caffeine Drugs 0.000 description 7
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 7
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- 230000004083 survival effect Effects 0.000 description 5
- 229930003270 Vitamin B Natural products 0.000 description 4
- 239000005018 casein Substances 0.000 description 4
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 4
- 235000021240 caseins Nutrition 0.000 description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 description 4
- 235000019156 vitamin B Nutrition 0.000 description 4
- 239000011720 vitamin B Substances 0.000 description 4
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- 238000011161 development Methods 0.000 description 2
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- 235000013305 food Nutrition 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
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- 241000254173 Coleoptera Species 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 240000006023 Trichosanthes kirilowii Species 0.000 description 1
- 235000009818 Trichosanthes kirilowii Nutrition 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 125000004403 catechin group Chemical group 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229960001617 ethyl hydroxybenzoate Drugs 0.000 description 1
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 description 1
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000013538 functional additive Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- 235000013619 trace mineral Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a pure artificial feed suitable for feeding and experimental analysis of tea geometrid larvae, and belongs to the technical field of insect feeds. The artificial feed of the invention adds epigallocatechin gallate (EGCG) into the existing artificial feed formula. The artificial feed has the advantages of low cost, easy management, better effect and no limitation of feed sources, the pupation rate of the larvae can reach 76-91 percent, the average pupation weight can reach 99-130 mg, the eclosion rate can reach more than 90 percent when the artificial feed is used for feeding tea-eating lepidoptera larvae, and the artificial feed is suitable for feeding tea-eating lepidoptera larvae, has simple and definite components, is suitable for experimental treatment and can achieve the effect similar to that of the artificial feed containing tea powder.
Description
Technical Field
The invention relates to a pure artificial feed suitable for feeding and experimental analysis of tea geometrid larvae, and belongs to the technical field of insect feeds.
Background
Tea geometrid is commonly known as Arch beetle, Arch back worm and Trichosanthes Kirilowii. Is one of the main pests of Chinese tea trees. Because the insect has more generations, is fast in propagation and spread, and is easy to outbreak and cause disasters. The Yangtze river region is seriously affected by tea production in southern provinces, especially in Jiang, Zhe, Wan, Xiang and other provinces. In order to effectively develop the technical research of the tea geometrid control, a large number of tea geometrids with consistent development need to be continuously cultured indoors, so that the artificial feeding of the tea geometrids is an important basis for the technical research of the control.
Most of the current artificial feed for tea geometrid contains higher content of tea powder, for example, in the research of the artificial feed for tea geometrid of plum cloud longevity in 1992, the tea powder accounts for 15.6% of the total amount, and in the research of the artificial feed for tea geometrid of Tangmeijun in 2017, CN 201710196737-the artificial feed for tea geometrid, the preparation method and the feeding method for tea geometrid, the tea powder accounts for 12.5% of the total amount. However, in the experimental study of the feeding of the tea geometrid, a single component or a known component is usually added into the feed to feed the tea geometrid so as to analyze which component affects the tea geometrid. The tea powder has complex components, and if the tea geometrid is fed by the feed containing the tea powder, the complex components in the tea powder cause troubles to the analysis problem.
201510116783.0 applied by inventor Zhang Yongan in 2005 discloses a pure artificial feed for feeding tea geometrid, which does not contain tea factors and lacks tea powder; however, the results show that the effect of the artificial feed on feeding the tea geometrid larvae is obviously reduced. For example, in example 1 of the Tangmeijun patent, the survival rate of the first experiment of the feed-fed tea geometrid is 95.7%, while the survival rate of the best tea geometrid in the feed formula for Yongan is only 73.6%. The survival rate is reduced, and the efficiency of the tea geometrid feeding research is also reduced.
Therefore, there is a need to develop a feed formula which has no tea powder with complex components and can ensure a good feeding effect of tea geometrid so as to solve the problems.
Disclosure of Invention
In order to solve the problems, the invention develops the artificial feed which is low in cost, easy to manage, better in effect, free from the limitation of feed sources and free from any tea powder, and meanwhile, the artificial feed can also be used for stably and artificially feeding tea geometrid for experimental treatment for a long time so as to achieve the feeding effect similar to that of the tea powder-containing artificial feed.
The first purpose of the invention is to provide an artificial feed for the artificial feeding and passage of tea geometrid larvae, which comprises, by mass, 12-20 parts of wheat germ, 2-3 parts of cane sugar, 4.5-5.5 parts of soybean meal, 1.5-2 parts of agar, 0.05-0.1 part of choline chloride, 0.5-1.5 parts of weber's salt, 0.03-0.4 part of sorbic acid, 0.25-0.3 part of nipagin, 0.2-1 part of ascorbic acid, 0.01-0.2 part of yeast powder, 0.5-2.5 parts of epigallocatechin gallate (EGCG), 0.05-0.15 part of cholesterol and 0.01-0.05 part of inositol.
In one embodiment of the invention, the artificial feed further comprises 66-79 parts of water.
In one embodiment of the invention, the wheat germ and the soybean meal are ground and sieved by a sieve of 80-120 meshes.
In one embodiment of the invention, the artificial feed comprises, by mass, 12 parts of wheat germ, 2 parts of sucrose, 4.5 parts of soybean meal, 2 parts of agar, 0.05 part of choline chloride, 0.5 part of wecker's salt, 0.03 part of sorbic acid, 0.25 part of nipagin, 0.2 part of ascorbic acid, 0.2 part of yeast powder, 0.5-2.5 parts of epigallocatechin gallate (EGCG), 0.1 part of cholesterol, and 0.035 part of inositol.
In the above formula, the nipagin can be methyl paraben, ethyl paraben, etc.
In one embodiment of the invention, the preparation method of the feed comprises the following specific steps:
(1) weighing agar according to a certain proportion, adding water according to a certain proportion, boiling for 1-2 min, sequentially adding wheat germ powder, soybean meal and sucrose according to a certain proportion, stirring uniformly, boiling, and cooling to 50-60 ℃;
(2) sequentially adding choline chloride, wecker salt, sorbic acid, methyl paraben, cholesterol and inositol according to a certain proportion, cooling to 20 ℃ while stirring, weighing ascorbic acid, yeast powder, choline chloride and EGCG according to a certain proportion, and uniformly stirring until the mixture is coagulated into a solid to obtain the compound feed.
In one embodiment of the invention, the EGCG is 70% pure.
The second purpose of the invention is to provide a method for feeding tea-eating lepidoptera larvae, which utilizes the artificial feed of the invention.
In one embodiment of the invention, the method for feeding tea-feeding lepidopteran larvae comprises:
(1) cutting the prepared artificial feed into slices, and inversely sticking the slices on the upper layer of a clean insect breeding box;
(2) transferring the 1 st larva hatched initially to the larva breeding box;
(3) adding new feed according to water loss condition and feed taste until larva pupates;
(4) the pupae were weighed and transferred to a jar containing sand, sprayed with appropriate amount of water to keep moist until eclosion.
In one embodiment of the present invention, in the step (3), the frequency of replacing the feedstuff is 2 to 3 days.
In an embodiment of the present invention, the method specifically includes: cutting the prepared feed into slices, inversely sticking the slices on the upper layer of the disinfected insect box, transferring the 1 st larva hatched initially into the insect box, and taking the feed by utilizing the phototropism of the ectropis obliqua. And (3) putting the insect breeding box into a climate chamber with the temperature of 23 +/-1 ℃ and the relative humidity of 75 +/-5 percent and the photoperiod L: D of 16:8 until the larvae pupate. The pupae were weighed and transferred to a jar containing sand, sprayed with appropriate amount of water to keep moist until eclosion.
The third purpose of the invention is to provide a method for analyzing the influence of unknown ingredients on the feeding effect of tea geometrid, the method comprises the steps of adding ingredients to be evaluated into the artificial feed to obtain mixed feed, feeding tea-eating lepidoptera larvae with the mixed feed, and comparing the feeding effects of the mixed feed and the artificial feed to obtain the influence of the ingredients to be evaluated on the feeding of the tea geometrid.
The invention has the beneficial effects that:
the pure artificial feed has the following advantages:
(1) low cost, easy management, better effect and no limitation of feed sources.
(2) The feed is used for feeding tea-eating lepidoptera larvae, the pupation rate of the larvae can reach 76-91 percent, the average pupation weight can reach 99-130 mg, and the eclosion rate can reach more than 90 percent, so the feed is suitable for feeding tea-eating lepidoptera larvae.
(3) The components are simple and clear, and the artificial feed is suitable for experimental treatment and can achieve the effect similar to that of the tea powder-containing artificial feed.
Detailed Description
The wheat germ, the casein and the ascorbic acid are all feed grades and are purchased in feed trade companies or retail stores; EGCG, caffeine, vitamin B, sorbic acid, methyl paraben and agar are all food grade and purchased from food additive company or store.
Example 1:
the improvement process of the pure artificial feed for the tea geometrid is divided into several stages. Firstly, the inventor finds that the growth condition of tea geometrid is obviously improved after the casein is replaced by the soybean meal; the inventor then replaces and increases the trace elements in the formula, and the improvement process is as follows:
the preparation method of the tea geometrid larva feed by adopting the formula (the formula 5 with the highest survival rate in the table 1, hereinafter referred to as the Zhang Yong original feed or the original feed) in 201510116783.0 and the improved feed is as follows:
the formula of the raw feed comprises: crushing the wheat germ, sieving the crushed wheat germ with a sieve of 80-120 meshes, weighing agar according to a proportion, adding water according to a proportion, boiling for 1-2 min, sequentially adding wheat germ powder and casein according to a proportion, stirring uniformly, boiling, and cooling to 50-60 ℃; sequentially adding the weberian salt, sorbic acid, methyl paraben and inositol according to a certain proportion, cooling to 20 ℃ while stirring, weighing and adding the ascorbic acid and the vitamin B according to a certain proportion, uniformly stirring until the ascorbic acid and the vitamin B are coagulated into solid, and storing at 4 ℃.
The improved feed formula comprises: crushing the wheat germ, sieving the crushed wheat germ with a sieve of 80-120 meshes, weighing agar according to a proportion, adding water according to a proportion, boiling for 1-2 min, sequentially adding wheat germ powder and soybean powder according to a proportion, stirring uniformly, boiling, and cooling to 50-60 ℃; sequentially adding sucrose, cholesterol, wecker salt, sorbic acid, methyl paraben and inositol in proportion, cooling to 20 ℃ while stirring, weighing ascorbic acid, choline chloride and yeast powder in proportion, uniformly stirring until the mixture is coagulated into solid, and storing at 4 ℃.
TABLE 1 proportions of Yongan raw feed and improved feed
| Raw feed ingredients | Mass percent (%) | Improved feed | Mass percent (%) |
| Wheat germ powder | 13.8 | Wheat germ powder | 12 |
| Casein as a food additive | 8 | Soybean powder | 4.5 |
| Sucrose | 2 | ||
| Agar-agar | 1.8 | Agar-agar | 2 |
| Choline chloride | 0.05 | ||
| Wechsler salt | 2.6 | Wechsler salt | 0.5 |
| Sorbic acid | 0.6 | Sorbic acid | 0.03 |
| Nipagin | 0.5 | Nipagin | 0.25 |
| Ascorbic acid | 1.0 | Ascorbic acid | 0.2 |
| Vitamin B | 0.9 | Yeast powder | 0.2 |
| Cholesterol | 0.1 | ||
| Inositol | 0.8 | Inositol | 0.035 |
| Water (W) | 70 | Water (W) | 78 |
Respectively cutting the prepared feed into slices, putting the slices into an insect breeding box, and transferring the 1 st larva hatched initially into the insect breeding box; and (4) changing new feed into the feed every 2-3 days until the larva pupates. The pupae were weighed and transferred to a jar containing sand, sprayed with appropriate amount of water to keep moist until eclosion.
The situation of using different formulations for feeding tea geometrid is shown in table 2:
TABLE 2 Life parameters for feeding tea geometrid larvae with different feeds
According to the table 2, after the Zhang Yong' an feed formula is optimized, the pupation rate, the average pupation weight and the eclosion rate of the tea geometrid larvae are slightly improved, and the duration of the larvae is correspondingly shortened.
Example 2:
although the feeding effect of example 1 is obviously improved, the effect of the artificial feed for the tea geometrid cannot be expected. The inventor unexpectedly finds that the feeding effect of the tea geometrid larvae is obviously improved by adding the EGCG into the feed, and the aim of continuously supplying a large amount of tea geometrid required by experimental treatment all year round can be achieved.
The epigallocatechin gallate (EGCG for short) which is a main additive in the pure artificial feed for the larvae of the tea geometrid in the embodiment is a catechin monomer separated from tea leaves and is a main component of the biological activity of tea polyphenol. EGCG is used in food industry for antioxidation, freshness preservation and deodorization, and in medicine industry for antibiosis, anticancer and anti-aging, and is used as a special functional additive in daily chemical products. However, in the insect world, polyphenol is generally toxic to insects, and due to the toxicity of polyphenol, no artificial feed suitable for subculture of insects is added with polyphenol at present. However, in the research process of the inventor, the EGCG is unexpectedly found that the addition of the EGCG to the feed for feeding the tea geometrid can effectively improve the survival rate of the tea geometrid, which is probably related to the EGCG improving the activity of the immune related enzyme phenol oxidase in the larvae of the tea geometrid. Therefore, in the present example, the inventors tried to add EGCG, a phenolic substance that is toxic in general knowledge, to artificial feed.
Through experimental verification of a preparation method and a feeding method similar to those in example 1, according to tables 3 and 4, when the addition amount of the EGCG in the feed reaches 0.5%, the feeding state of the tea geometrid larvae starts to be obviously improved, and the effect is better along with the increase of the addition amount of the EGCG. However, when the addition amount of the EGCG reaches 3%, the feeding effect of the tea geometrid larvae begins to be greatly reduced. Therefore, we finally determined that the optimal EGCG addition ratio ranges from 0.5% to 2.5%.
TABLE 3 tea geometrid larva pure artificial feed with different proportions of EGCG
TABLE 4 influence of different ECGC addition amounts on tea geometrid
Meanwhile, the inventor also compares the influence of other tea extracts on tea geometrid. The inventor adds caffeine to the feed formula in table 3 to replace EGCG, and as a result, the inventor finds that the growth and development of tea geometrid is influenced by feeding tea geometrid (the addition amount of EGCG and caffeine in the feed is between the content range of the agricultural anti-early fresh leaves). Specific results are shown in table 5.
TABLE 5 Effect of caffeine on the Artificial rearing of tea geometrid larvae
As can be seen from Table 5, when caffeine was added to the feed to feed tea geometrid, the feeding effect was rather decreased. When the caffeine is added into the feed to feed the tea geometrid larvae, the pupation rate of the larvae is obviously reduced and even far lower than that of the original feed. The caffeine added into the feed can make the tea geometrid larva grow abnormally, pupate in the later stage of 5 years, shorten the pupation period, reduce pupal weight and make the adult deformed.
Example 3:
selecting 90 heads of tea geometrid larvae which are hatched initially in the same batch, and breeding under the climate chamber conditions of the temperature (23 +/-1) DEG C, the relative humidity of 75 +/-5 percent and the photoperiod L: D of 16: 8. And (3) inoculating the newly hatched larvae into an insect breeding box (30 heads in each box), feeding the artificial feed according to the formula 3, and changing new feed into the newly hatched larvae every 2-3 days after the water loss condition and the feed taste are shown until the larvae pupate. The pupae were weighed and transferred to a jar containing sand, sprayed with appropriate amount of water to keep moist until eclosion.
The pupation rate of the larva fed by the formula is 84.6%, the gradual period of the larva is 22.3 days, the average pupal weight is 101.6mg, and the eclosion rate is 92.3%.
Example 4:
the same batch of tea geometrid is selected to hatch 90 heads of first instar larvae for the first time, and the artificial feed, the breeding method and the conditions are the same as those of the example 3 according to the formula 4.
The pupation rate of the larvae fed by the formula is 88.2 percent, the gradual period of the larvae is 21.3 days, the average pupal weight is 109.6mg, and the eclosion rate is 92.5 percent.
Example 5:
the same batch of tea geometrid is selected to hatch 90 heads of first instar larvae for the first time, and the artificial feed, the breeding method and the conditions are the same as those of the example 3.
The pupation rate of the larva fed by the formula is 90.6%, the gradual period of the larva is 21.8 days, the average pupal weight is 121.4mg, and the eclosion rate is 95.3%.
Claims (10)
1. The artificial feed for artificial feeding of tea geometrid larvae is characterized by comprising 12-20 parts by mass of wheat germs, 2-3 parts by mass of sucrose, 4.5-5.5 parts by mass of soybean meal, 1.5-2 parts by mass of agar, 0.05-0.1 part by mass of choline chloride, 0.5-1.5 parts by mass of wechsler's salt, 0.03-0.4 part by mass of sorbic acid, 0.25-0.3 part by mass of nipagin, 0.2-1 part by mass of ascorbic acid, 0.01-0.2 part by mass of yeast powder, 1-2.5 parts by mass of epigallocatechin gallate (EGCG), 0.05-0.15 part by mass of cholesterol and 0.01-0.05 part by mass of inositol.
2. The artificial feed according to claim 1, wherein the artificial feed further comprises 66-79 parts of water.
3. The artificial feed according to claim 1, wherein the wheat germ and the soybean meal are crushed and sieved with a sieve of 80-120 meshes.
4. The artificial feed according to claim 1, which comprises, in parts by mass, 12 parts of wheat germ, 2 parts of sucrose, 4.5 parts of soybean meal, 2 parts of agar, 0.05 part of choline chloride, 0.5 part of wechsler's salt, 0.03 part of sorbic acid, 0.25 part of nipagin, 0.2 part of ascorbic acid, 0.2 part of yeast powder, 1-2.5 parts of epigallocatechin gallate (EGCG), 0.1 part of cholesterol, and 0.035 part of inositol.
5. The artificial feed according to claim 1, wherein the feed preparation method comprises the following specific steps:
(1) weighing agar according to a certain proportion, adding water according to a certain proportion, boiling for 1-2 min, sequentially adding wheat germ powder, soybean meal and sucrose according to a certain proportion, stirring uniformly, boiling, and cooling to 50-60 ℃;
(2) sequentially adding choline chloride, wecker salt, sorbic acid, methyl paraben, cholesterol and inositol according to a certain proportion, cooling to 20 ℃ while stirring, weighing ascorbic acid, yeast powder, choline chloride and EGCG according to a certain proportion, and uniformly stirring until the mixture is coagulated into a solid to obtain the compound feed.
6. A method of feeding tea-feeding lepidopteran larvae, which comprises using the artificial feed according to any one of claims 1 to 5.
7. The method according to claim 6, characterized in that the method is:
(1) cutting the prepared artificial feed into slices, and inversely sticking the slices on the upper layer of a clean insect breeding box;
(2) transferring the 1 st larva hatched initially to the larva breeding box;
(3) adding new feed according to water loss condition and feed taste until larva pupates;
(4) the pupae were weighed and transferred to a jar containing sand, sprayed with appropriate amount of water to keep moist until eclosion.
8. The method according to claim 7, characterized in that the method is: in the step (3), the frequency of replacing the feed is once every 2 to 3 days.
9. The method according to claim 6, characterized in that it is in particular: cutting the prepared feed into slices, inversely sticking the slices on the upper layer of the disinfected insect box, transferring the 1 st larva hatched initially into the insect box, and taking the feed by utilizing the phototropism of the ectropis obliqua; putting the insect breeding box into a climate chamber with the temperature of 23 +/-1 ℃ and the relative humidity of 75 +/-5 percent and the photoperiod L: D =16:8 until the larva pupates; the pupae were weighed and transferred to a jar containing sand, sprayed with appropriate amount of water to keep moist until eclosion.
10. A method for analyzing the influence of unknown ingredients on the feeding effect of tea geometrid is characterized in that the ingredients to be evaluated are added into the artificial feed according to any one of claims 1 to 5 to obtain mixed feed, the mixed feed is used for feeding tea-eating lepidoptera larvae, and the feeding effect of the ingredients to be evaluated on the feeding of the tea geometrid is obtained by comparing the feeding effect of the mixed feed and the feeding effect of the artificial feed.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104920868A (en) * | 2015-06-10 | 2015-09-23 | 华南农业大学 | Application of epi-gallocatechin-gallate (EGCG) in respect of preparing pig feed additive |
| CN106819664A (en) * | 2017-03-30 | 2017-06-13 | 青岛海之星生物科技有限公司 | A kind of tea geometrid larvae artificial feeding feed and preparation method thereof |
| CN106962695A (en) * | 2017-03-29 | 2017-07-21 | 中国农业科学院茶叶研究所 | A kind of tea geometrid man-made feeds, preparation method and tea geometrid method for breeding |
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| CN104920868A (en) * | 2015-06-10 | 2015-09-23 | 华南农业大学 | Application of epi-gallocatechin-gallate (EGCG) in respect of preparing pig feed additive |
| CN106962695A (en) * | 2017-03-29 | 2017-07-21 | 中国农业科学院茶叶研究所 | A kind of tea geometrid man-made feeds, preparation method and tea geometrid method for breeding |
| CN106819664A (en) * | 2017-03-30 | 2017-06-13 | 青岛海之星生物科技有限公司 | A kind of tea geometrid larvae artificial feeding feed and preparation method thereof |
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| 茶尺蠖人工饲料营养成分及饲养结果的分析;袁争等;《茶业通报》;20171231;第39卷(第1期);第28-33页 * |
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