CN107801876B - Artificial feed for adult cantharis, and preparation method and application thereof - Google Patents
Artificial feed for adult cantharis, and preparation method and application thereof Download PDFInfo
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- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides an artificial feed for adult cantharis, a preparation method and application thereof, and belongs to the technical field of insect breeding. The artificial feed comprises the following components in parts by weight: 70-75 parts of water, 5-10 parts of soybean meal, 5-10 parts of yeast powder, 5-10 parts of pollen, 4-5 parts of vitamin glucose, 1-5 parts of ascorbic acid, 0.01-0.02 part of preservative, 0.5-1.0 part of royal jelly, 0.5-1.0 part of honey and 1-3 parts of agar powder. The artificial feed formed by selecting the components ensures that the carbohydrate, the protein and the fatty acid required in the growth process of the adult cantharis are ensured, the adult cantharis is not dependent on flowers of leguminous families for feeding, and the normal growth and development of the adult cantharis are ensured and the service life of the adult cantharis is also ensured after the artificial feed provided by the invention is fed. Compared with natural feed, the artificial feed provided by the invention has similar effects in terms of days in the early stage of spawning and the spawning amount.
Description
Technical Field
The invention belongs to the technical field of insect breeding, and particularly relates to an artificial feed for adult cantharis, and a preparation method and application thereof.
Background
Mylabris phalerata, also known as lilac daphne genkwa, belongs to the family of the order of Coleoptera and genkwa, is generally called as Mylabris with southern Mylabris phalerata (daphne genkwa), is an animal drug recorded in Chinese pharmacopoeia (2000), has the functions of breaking up illness and dissipating stagnation, and attacking toxin and removing sores, and has the cantharidin of resisting cancers and viruses. Stimulating leucopoiesis, inhibiting fungi and the like, and is an important anticancer and antiviral traditional Chinese medicinal material at present. However, due to the destruction of forest vegetation, the change of natural environment, the reduction of the planting area of leguminous plants and cucurbitaceae plants and the disordered mass capture of human beings, the wild cantharis resources are continuously reduced and can not meet the requirements of market medicine far away, so that the cantharidin is one of the most scarce animal medicinal materials, the condition for artificially synthesizing the cantharidin is very difficult (the cantharidin needs to be carried out under the extremely high pressure of 1.520 Gpa), and the contradiction of the supply-demand relationship of the cantharidin is stronger, so the artificial scale feeding of the cantharidin insects is the key for solving the contradiction.
At present, the literature about the research of the cantharis feeding technology is few, and the reports of artificial large-scale cultivation in production practice are not available. The insects mostly occur in the field for one generation in 1 year, about 6-7 months in a lifetime are in the development stopping stage, the life cycle is long, and the reproduction speed of the cantharis is greatly reduced. At present, most scholars in China usually adopt natural feed plants which are favored by cantharis to feed adult cantharis, for example, chen jiang et al report that the feeding habits and feeding modes of adult mylabris cantoniensis and yellow black mylabris cantoniensis are studied, and find that flowers of the adult mylabris cantoniensis are favored by plants, particularly leguminous plants, and the feeding habits are as follows: soybean flower, cowpea flower, luffa flower, pumpkin flower. Wang huilong et al have conducted preliminary research on adult yellow and black cantharis feed, but the main component of the feed is to use fresh luffa flowers to be added into the feed after freeze drying, so that a large amount of luffa plants are required to be planted to pick the luffa flowers for freeze drying, and the luffa flowers are added into the feed, so that the feeding cost is greatly increased.
Disclosure of Invention
In view of the above, the invention aims to provide an artificial feed for adult cantharis, a preparation method and an application thereof, wherein the artificial feed breaks through the habit that the adult cantharis depends on flowers of leguminous plants and cucurbitaceae plants under the natural condition, realizes the purpose of large-scale long-term subculture, and reduces the feeding cost.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an artificial feed for adult cantharis, which comprises the following components in parts by weight: 70-75 parts of water, 5-10 parts of soybean meal, 5-10 parts of yeast powder, 5-10 parts of pollen, 4-5 parts of vitamin glucose, 1-5 parts of ascorbic acid, 0.01-0.02 part of preservative, 0.5-1.0 part of royal jelly, 0.5-1.0 part of honey and 1-3 parts of agar powder.
Preferably, the composition comprises the following components in parts by weight: 72-74 parts of water, 6-8 parts of soybean meal, 6-9 parts of yeast powder, 7-9 parts of pollen, 4.2-4.8 parts of vitamin glucose, 2-4 parts of ascorbic acid, 0.012-0.018 part of preservative, 0.6-0.8 part of royal jelly, 0.6-0.8 part of honey and 1.5-2.5 parts of agar powder.
Preferably, the composition comprises the following components in parts by weight: 73 parts of water, 7 parts of soybean meal, 8 parts of yeast powder, 8 parts of pollen, 4.5 parts of multi-vitamin glucose, 3 parts of ascorbic acid, 0.015 part of preservative, 0.7 part of royal jelly, 0.7 part of honey and 2 parts of agar powder.
Preferably, the pollen is corn pollen.
Preferably, the preservative is sorbic acid and/or paraben.
The invention provides a preparation method of an artificial feed for adult cantharis, which comprises the following steps:
1) mixing soybean powder and yeast powder, and sterilizing for 30min to obtain sterilized material;
2) mixing the sterilized material obtained in the step 1) with ascorbic acid, pollen, multivitamin glucose, a preservative, royal jelly, honey and 35-40% of water to obtain a mixed material;
3) dissolving agar powder and the rest water at 100 ℃ to obtain an agar liquid;
4) mixing and stirring the mixed material obtained in the step) and the agar liquid obtained in the step 2) to obtain an artificial feed;
there is no chronological restriction between the step 1) and the step 2).
Preferably, the temperature of sterilization is 121 ℃.
Preferably, the step 3) of mixing and stirring further comprises cooling and dicing.
The invention provides the application of the artificial feed or the artificial feed prepared by the method in feeding cantharis adults.
Preferably, the cantharis is yellow black cantharis.
Preferably, the feeding amount of the artificial feed is 14.19-15.35 mg/head/time; the number of days for feeding the artificial feed is 61-68 d; feeding for 2-3 times every day.
The artificial feed for the cantharis adults provided by the invention comprises the following components in parts by weight: 70-75 parts of water, 5-10 parts of soybean meal, 5-10 parts of yeast powder, 5-10 parts of pollen, 4-5 parts of vitamin glucose, 1-5 parts of ascorbic acid, 0.01-0.02 part of preservative, 0.5-1.0 part of royal jelly, 0.5-1.0 part of honey and 1-3 parts of agar powder. In the artificial feed provided by the invention, water meets the water requirement of the adult cantharis; the soybean flour meets the requirements of cantharis on protein, fatty acid, cholesterol, tocopherol, various vitamins and other nutrients necessary for the growth of insects; the yeast powder meets the requirements of the cantharis adults on protein, nucleic acid and saccharide; the pollen provides insect protein, vitamins and microelements, balances the nutrition in vivo, and enhances metabolism; the vitamin glucose meets the requirements of the cantharides imago on carbohydrate, and the ascorbic acid is beneficial to stimulating the food intake; the royal jelly meets the requirements of the adult cantharis on amino acid, B vitamins and sterol substances; the honey satisfies the requirement of cantharides for carbohydrate. The artificial feed formed by selecting the components ensures that the carbohydrate, the protein and the fatty acid required in the growth process of the adult cantharis are ensured, the adult cantharis is not dependent on leguminous flowers to eat, and the normal growth and development of the adult cantharis are ensured and the service life of the adult cantharis is also ensured after the artificial feed provided by the invention is fed.
Meanwhile, the artificial feed for the adult cantharis provided by the invention enables the early stage of egg laying of the raised adult cantharis to reach 21.2 days, the egg laying amount to be 17.85 pieces, and the average egg grain of each piece is 31.4 grains; after the adult cantharis is fed by the luffa flower, the average service life of the adult cantharis is 60.5 days, the early stage of egg laying is 20.28 days, the egg laying amount is 18.15 pieces, and the average egg grain of each piece is 32.15 grains. Compared with natural feed, the artificial feed provided by the invention has similar effects on days in the early stage of spawning and the spawning amount. Therefore, the artificial feed provided by the invention can meet the requirement of adult cantharis on nutrition, solves the problems of time, labor, cost and the like required by planting, picking and fresh-keeping of the luffa flower crops, is not limited by seasons, has rich raw material sources, is easy to obtain, greatly reduces the cantharis cultivation cost, and gradually realizes the large-scale cantharis cultivation.
Detailed Description
The invention provides an artificial feed for adult cantharis, which comprises the following components in parts by weight: 70-75 parts of water, 5-10 parts of soybean meal, 5-10 parts of yeast powder, 5-10 parts of pollen, 4-5 parts of vitamin glucose, 1-5 parts of ascorbic acid, 0.01-0.02 part of preservative, 0.5-1.0 part of royal jelly, 0.5-1.0 part of honey and 1-3 parts of agar powder.
The artificial feed for adult cantharis provided by the invention comprises soybean meal. The soybean flour is 5-10 parts by weight, preferably 6-8 parts by weight, and more preferably 7 parts by weight. The particle size of the soybean powder is preferably 50-100 meshes, and more preferably 80 meshes. The soybean powder meets the requirements of cantharis on protein, fatty acid, cholesterol, tocopherol, various vitamins and other nutrients necessary for the growth of insects. The source of the soy flour is not particularly limited in the present invention and may be any source known in the art.
The artificial feed for adult cantharis provided by the invention comprises pollen. The pollen is 5-10 parts by weight, preferably 7-9 parts by weight, and more preferably 8 parts by weight. The pollen is preferably corn pollen. Pollen provides insect protein, vitamins, and microelements, balances nutrition in vivo, and enhances metabolism. The source of the pollen is not particularly limited in the present invention, and any known pollen source in the art may be used. In the embodiment of the invention, the pollen is purchased from Yimeng Liuchangkun big bee field company.
The artificial feed for the cantharis adults provided by the invention comprises yeast powder. The yeast powder is 5-10 parts by weight, preferably 6-9 parts by weight, and more preferably 8 parts by weight. The yeast powder meets the requirements of the cantharis adults on protein, nucleic acid and saccharide. The source of the yeast powder is not particularly limited in the present invention, and the yeast powder known in the art can be used. In the embodiment of the invention, the yeast powder is Angel yeast powder.
The artificial feed for the cantharis adults provided by the invention comprises multi-vitamin glucose. The multivitamin glucose is 4-5 parts by weight, preferably 4.2-4.8 parts by weight, and more preferably 4.5 parts by weight. The multidimensional glucose meets the requirement of the cantharis imago on carbohydrate. The source of the multidimensional glucose is not particularly limited in the present invention, and any source of multidimensional glucose known in the art may be used. In the embodiment of the invention, the multidimensional glucose is purchased from Chongqing and Ping pharmaceutical Co.
The artificial feed for the cantharis adults provided by the invention comprises ascorbic acid. The ascorbic acid is 1-5 parts by weight, preferably 2-4 parts by weight, and more preferably 3 parts by weight. The ascorbic acid is a nutrient component necessary for the growth of the adult cantharis and is also a food stimulant. The source of the ascorbic acid in the present invention is not particularly limited, and any ascorbic acid known in the art may be used. In the examples of the present invention, the ascorbic acid was purchased from a chemical reagent factory of Syngnathus.
The artificial feed for the cantharis adults provided by the invention comprises a preservative. The preservative is 0.01-0.02 part by weight, preferably 0.012-0.018 part by weight, and more preferably 0.015 part by weight. The antiseptic is used for feed preservation. When the preservative is sorbic acid and nipagin, the mass ratio of the sorbic acid to the nipagin is preferably 1: 1. The source of the preservative is not particularly limited in the present invention, and any source of preservatives known in the art may be used. In the embodiment of the invention, the preservative is purchased from a Dougulong chemical reagent factory.
The artificial feed for adult cantharis provided by the invention comprises royal jelly. The royal jelly is 0.5-1.0 part by weight, preferably 0.6-0.8 part by weight, and more preferably 0.7 part by weight. The royal jelly meets the requirements of the adult cantharis on amino acid, B vitamins and sterol substances. The source of the royal jelly is not particularly limited, and the source of the royal jelly known in the field can be adopted. In the embodiment of the invention, the royal jelly is purchased from Yipinchongfang bee products company.
The artificial feed for adult cantharis provided by the invention comprises honey. The honey accounts for 0.5-1.0 part by weight, preferably 0.6-0.8 part by weight, and more preferably 0.7 part by weight. The honey satisfies the requirement of cantharides for carbohydrate. The source of the honey is not particularly limited in the present invention, and any source of honey known in the art may be used. In the examples of the present invention, the honey was purchased from guangyuan bee products, ltd.
The artificial feed for adult cantharis provided by the invention comprises agar powder. The agar powder is 1-3 parts by weight, preferably 1.5-2.5 parts by weight, and more preferably 2 parts by weight. The agar powder provides a solidifying agent for the feed and provides water for the feed. The source of the agar powder is not particularly limited in the present invention, and any known source of agar powder in the art may be used. In the embodiment of the invention, the agar powder is purchased from a chemical reagent factory of Doxolone.
The artificial feed for the adult cantharis provided by the invention comprises water. The water accounts for 70-75 parts by weight, and preferably 72 parts by weight. The water is used to dissolve other components while providing moisture required for the growth of cantharis. The source of the water is not particularly limited in the present invention, and any source of water known in the art may be used.
The invention provides a preparation method of an artificial feed for adult cantharis, which comprises the following steps:
1) mixing soybean powder and yeast powder under high pressure sterilization at 121 deg.C for 30min to obtain sterilized material;
2) mixing the sterilized material obtained in the step 1) with pollen, multivitamin glucose, ascorbic acid, a preservative, royal jelly, honey and 35-40% of water to obtain a mixed material;
3) dissolving agar powder and the rest water at 100 ℃ to obtain an agar liquid;
4) mixing and stirring the mixed material obtained in the step) and the agar liquid obtained in the step 2) to obtain an artificial feed;
there is no chronological restriction between the step 1) and the step 2).
The soybean flour and the yeast powder are mixed and sterilized for 30min under the high-pressure sterilization condition of 121 ℃, so that the sterilized material is obtained.
The method of sterilization is not particularly limited in the present invention, and a sterilization protocol well known to those skilled in the art may be used. The soybean flour generates aromatic flavor after being sterilized, which is beneficial to attracting the adult cantharis to eat; meanwhile, the soybean powder eliminates the toxicity of the soybean ketone in the soybean after sterilization, so that the cantharides is fed by the soybean powder more safely. The temperature for sterilization is preferably 121 ℃. The time for sterilization is preferably 30 min. After the sterilized materials are obtained, the invention mixes the sterilized materials with ascorbic acid, preservative, royal jelly and honey to obtain mixed materials. The mixing method is not particularly limited in the present invention, and a mixing scheme well known to those skilled in the art may be employed.
After the mixed material and the agar liquid are obtained, the mixed material and the agar liquid are mixed and stirred to obtain the artificial feed. The purpose of mixing and stirring is to uniformly mix the mixed material and the agar liquid.
In the present invention, after the mixing, it is preferable that the mixing further comprises: cooling and cutting the obtained feed liquid into blocks. Cooling the mixture to 20-35 ℃ and cutting into blocks. The particle size of the cut blocks is a cube with the side length of 0.8-1.2 cm.
The invention provides the application of the artificial feed or the artificial feed prepared by the method in feeding cantharis adults.
In the invention, the cantharis is preferably yellow black cantharis.
In the invention, the feeding amount of the artificial feed is preferably 14.19-15.35 mg/mg, and more preferably 14.20-15.10 mg/mg. The number of days for feeding the artificial feed is preferably 61-68 d, and more preferably 65 d. The number of times of feeding the artificial feed every day is 2-3.
The artificial feed for adult cantharis provided by the present invention, the preparation method and the application thereof are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Weighing the following components by weight: 70g of water, 10g of soybean meal, 5g of yeast powder, 10g of corn pollen, 4g of multivitamin glucose, 5g of ascorbic acid, 0.01g of sorbic acid, 1.0g of royal jelly, 0.5g of honey and 3g of agar powder.
(II) the preparation method of the artificial feed for the cantharis adults comprises the following steps:
1) sterilizing soybean powder and yeast powder at 121 deg.C for 30min to obtain sterilized material;
2) mixing the sterilized material obtained in the step 1) with pollen, multivitamin glucose, ascorbic acid, a preservative, royal jelly, honey and water accounting for 35% of the total mass to obtain a mixed material;
3) dissolving agar powder and the rest water at 100 ℃, mixing and stirring the obtained mixed solution and the mixed material, cooling and cutting into pieces to obtain the artificial feed.
Example 2
Weighing the following components by weight: 75g of water, 5g of soybean meal, 10g of yeast powder, 5g of corn pollen, 5g of multivitamin glucose, 1g of ascorbic acid, 0.02g of nipagin, 0.5g of royal jelly, 1.0g of honey and 1g of agar powder.
(II) the preparation method of the artificial feed for the cantharis adults comprises the following steps:
1) sterilizing soybean powder and yeast powder at 121 deg.C for 30min to obtain sterilized material;
2) mixing the sterilized material obtained in the step 1) with pollen, multivitamin glucose, ascorbic acid, a preservative, royal jelly, honey and water accounting for 40% of the total mass to obtain a mixed material;
3) dissolving agar powder and the rest water at 100 ℃, mixing and stirring the obtained mixed solution and the mixed materials, cooling and cutting into pieces to obtain the artificial feed.
Example 3
Weighing the following components by weight: 73g of water, 7g of soybean meal, 8g of yeast powder, 8g of corn pollen, 4.5g of multi-vitamin glucose, 3g of ascorbic acid, 0.015g of nipagin, 0.7g of royal jelly, 0.7g of honey and 2g of agar powder.
(II) the preparation method of the artificial feed for the cantharis adults comprises the following steps:
1) sterilizing soybean powder and yeast powder at 121 deg.C for 30min to obtain sterilized material;
2) mixing the sterilized material obtained in the step 1) with pollen, multivitamin glucose, ascorbic acid, a preservative, royal jelly, honey and 38% of water by mass of the total mass to obtain a mixed material;
3) dissolving agar powder and the rest water at 100 ℃, mixing and stirring the obtained mixed solution and the mixed materials, cooling and cutting into pieces to obtain the artificial feed.
Comparative example 1
Weighing the following components by weight: 73g of water, 7g of soybean meal, 8g of towel gourd pollen, 4.5g of multivitamin glucose, ascorbic acid, a preservative, 0.7g of honey, royal jelly and 2g of agar powder.
(II) the preparation method of the artificial feed for the cantharis adults comprises the following steps:
1) sterilizing soybean powder and yeast powder at 121 deg.C for 30min to obtain sterilized material;
2) mixing the sterilized material obtained in the step 1) with pollen, multivitamin glucose, ascorbic acid, a preservative, royal jelly, honey and water accounting for 37% of the total mass to obtain a mixed material;
3) and (3) mixing and stirring mixed liquid obtained by dissolving agar powder and the rest water at the temperature of 100 ℃ with the mixed material, cooling and cutting into blocks to obtain the artificial feed.
Example 4
The artificial feed for the cantharis adults prepared in the examples 1 to 3 is used for feeding 100 cantharis adults in 2L containers respectively at the indoor temperature of 30 ℃ and the illumination time of 15 hours, feeding the 100 cantharis adults with fresh luffa flowers as a positive control group once every 10:00 am and 16:00 pm, wherein the feeding amount is 15.35 g every day, and counting the service life and the egg laying amount of the cantharis adults fed in each group. The results are shown in Table 1.
TABLE 1 situation of the Mylabris adults fed with the artificial feed for Mylabris adults prepared in examples 1 to 3
The results in table 1 show that the artificial feed for cantharis adults has an average life of 61 days per container, 21.20 days in the early stage of egg laying, an egg laying amount of 17.85 pieces, an average number of 31.40 eggs per piece, a control luffa flower, an average adult life of 60.5 days per container, an average egg laying amount of 18.15 pieces, and an average number of 32.15 eggs per piece.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. The application of the artificial feed in prolonging the service life of the adult cantharis is characterized in that the artificial feed comprises the following components in parts by weight: 70-75 parts of water, 5-10 parts of soybean meal, 5-10 parts of yeast powder, 5-10 parts of corn pollen, 4-5 parts of multivitamin glucose, 1-5 parts of ascorbic acid, 0.01-0.02 part of nipagin, 0.5-1.0 part of royal jelly, 0.5-1.0 part of honey and 1-3 parts of agar powder.
2. The use according to claim 1, characterized by comprising the following components in parts by weight: 72-74 parts of water, 6-8 parts of soybean meal, 6-9 parts of yeast powder, 7-9 parts of corn pollen, 4.2-4.8 parts of multivitamin glucose, 2-4 parts of ascorbic acid, 0.012-0.018 part of nipagin, 0.6-0.8 part of royal jelly, 0.6-0.8 part of honey and 1.5-2.5 parts of agar powder.
3. The use according to claim 2, characterized by comprising the following components in parts by weight: 73 parts of water, 7 parts of soybean meal, 8 parts of yeast powder, 8 parts of corn pollen, 4.5 parts of multi-vitamin glucose, 3 parts of ascorbic acid, 0.015 part of nipagin, 0.7 part of royal jelly, 0.7 part of honey and 2 parts of agar powder.
4. The use according to claim 1, in a process for the preparation of artificial feed, comprising the steps of:
1) mixing soybean powder and yeast powder, and sterilizing for 30min to obtain sterilized material; mixing the sterilized material with ascorbic acid, corn pollen, multivitamin glucose, a preservative, royal jelly, honey and 35-40% of water to obtain a mixed material;
2) dissolving agar powder and the rest water at 100 ℃ to obtain an agar liquid;
3) mixing and stirring the mixed material obtained in the step 1) and the agar liquid obtained in the step 2) to obtain an artificial feed;
there is no chronological restriction between the step 1) and the step 2).
5. Use according to claim 4, wherein the temperature of sterilization in step 1) is 121 ℃.
6. The use of claim 1, wherein cantharis is yellow black cantharis.
7. The use of claim 1 or 6, wherein the artificial feed is fed in an amount of 14.19 to 15.35 mg/dose; the days for feeding the artificial feed are 61-68 d, and the artificial feed is fed for 2-3 times every day.
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| CN103621822B (en) * | 2013-11-19 | 2015-06-03 | 重庆市中药研究院 | Artificial feed for adult mylabris phalerata pallas and preparation method thereof |
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