CN102178095A - Artificial feed for mylabris cichorii imagoes and preparation method thereof - Google Patents
Artificial feed for mylabris cichorii imagoes and preparation method thereof Download PDFInfo
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- CN102178095A CN102178095A CN2011101044253A CN201110104425A CN102178095A CN 102178095 A CN102178095 A CN 102178095A CN 2011101044253 A CN2011101044253 A CN 2011101044253A CN 201110104425 A CN201110104425 A CN 201110104425A CN 102178095 A CN102178095 A CN 102178095A
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Abstract
The invention discloses an artificial feed for mylabris cichorii imagoes, which comprises the following components in percentage by weight: 7-15% of soybean meal, 3-10% of yeast powder, 4-10% of dry loofah flower, 3-7% of multivitamin glucose, 1-3% of pollen, 1-3% of honey, 60-70% of water, 1-2% of agar, and 0.1-0.5% of sorbic acid. The invention also provides a method for preparing the artificial feed for mylabris cichorii imagoes.
Description
Technical field
The present invention relates to a kind of feed of artificial feeding eye spot blister beetle adult and preparation method of this kind feed of being used for.
Background technology
Eye spot blister beetle (
Mylabris cichoriiLinnaeus) having another name called yellow black mylabris cichorii, is the animal-use drug that is written into China's pharmacopeia, belongs to Insecta coleoptera Meloidae insect.Eye spot blister beetle belongs to the hypermetamorphism insect, and its history of life is unique and complicated, and experience ovum, larva, pupa and four stages of development of adult all one's life.Eye spot blister beetle life cycle is long, and next is only field condition and took place for 1 generation, with hibernate of ovum.Eye spot blister beetle adult occurs in China general 6～August, is food with fabaceous flower, also can get the flower of food cucurbitaceous plant; Eye spot blister beetle larva has 6 stages of development, lives in the soil, and be food with the locust ovum, be effective natural enemy resource insect.
Eye spot blister beetle contains cantharidin (Cantharidin, C because of its adult and larva body
10H
12O
4) and have important medical value.Modern medicine study finds that cantharidin is a kind of effective natural anti-cancer drugs, simultaneously some difficult and complicated cases is also had unique curative effect.Cantharidin is evident in efficacy to the treatment primary carcinoma of liver, also be used to treatment or diseases such as supplemental treatment intestinal cancer, lung cancer, breast cancer, cancer of the esophagus, cirrhosis and neurodermatitis, can be used for treating scytitises such as mange simultaneously, be to use more broad spectrum activity anticarcinogen and external preparation for skin medicine a few days ago.To studies show that of cantharidin and antitumaous effect thereof, the cantharidin anticancer mechanism mainly is by suppressing the activity of phosphorylase in the cancer cell, thereby regulates protein expression, causes cell-cycle arrest, to reach the growth that suppresses tumour cell and the purpose of breeding.
Along with cantharidin application more and more widely, demand to the Meloidae insect also increases day by day, but because the change of ecological environment and excessive the collection are constantly reduced wild cantharidin resource, cause its wild resource scarcity, hindered the development and utilization of cantharidin resource.Therefore artificial large-scale cultivation becomes the inexorable trend that solves cantharidin shortage of resources problem.But the food of following spot blister beetle adult of natural conditions is the flower of fabaceous flower or cucurbitaceous plant, because spending of these plants can not all can be opened throughout the year, even flower is arranged, the cost of plucking legume is very high, also can influence the output of beans after perianth is plucked; In addition, the nutritive loss in the preservation of fresh flower and the storage life also is a difficult problem.Therefore, the research and development of eye spot blister beetle adult man-made feeds and preparation are problems that realizes that at present eye spot blister beetle large-scale breeding is badly in need of solving.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of spot blister beetle adult man-made feeds prescription, this prescription can live, can raise in the anniversary so that an eye spot blister beetle is broken away from fresh flower and feeding cost low; The present invention simultaneously also provides the preparation method of this kind feed.
Technical scheme of the present invention is: a kind of spot blister beetle adult man-made feeds, its prescription is made up of each component of following percentage by weight: analysis for soybean powder 7%～15%, dusty yeast 3%～10%, shredded dried bean curd melon spend 4%～10%, multivita-glucose 3%～7%, pollen 1%～3%, honey 1%～3%, water 60%～70%, agar 1%～2% and sorbic acid 0.1%～0.5%.
Adopt above-mentioned feed formula, analysis for soybean powder wherein can provide protein for eye spot blister beetle; Dusty yeast provides nutrition such as organic nitrogen source and vitamin, auxin for eye spot blister beetle; Multivita-glucose provides carbohydrate and vitamin; Honey can provide multiple organic acids such as inorganic salts, vitamin, iron, calcium, copper, manganese, potassium, phosphorus and some trace elements and fructose, glucose, amylase, oxidizing ferment, reductase or the like.And sponge gourd flower is the natural feed of eye spot blister beetle, wherein contains vitamin, protein, organic acid, carbohydrate and attracts eye spot blister beetle to get the attractant etc. of food.Therefore product of the present invention can substitute the food of fresh flower as eye spot blister beetle, and more nutritious than fresh flower, can satisfy the needs of growth, growth and the mate and oviposit of eye spot blister beetle.
The feed that technique scheme is made with the analysis for soybean powder that is easy to get, dusty yeast etc. replaces a large amount of fresh flowers, has saved cost greatly; Simultaneously, sponge gourd flower flower is big, male flower can not grow fruit, and therefore appropriateness is plucked the output that can not influence sponge gourd, and raw material conveniently is easy to get.
Agar makes that above-mentioned various nutriment can the solidify out into solid in the such scheme, is easy to store, and an eye spot blister beetle imago feeding is convenient; Sorbic acid has the corrosion-resistant effect, makes product to preserve use by longer-term.
Described shredded dried bean curd melon flower can not influence the nutritional labeling of sponge gourd flower like this for male sponge gourd flower petal and the pistil of newly taking obtains through after the freeze-drying.
The preparation method of above-mentioned feed prepares as follows:
(a) get analysis for soybean powder and dusty yeast and pack into and mix in first beaker, seal laggard horizontal high voltage sterilization;
(b) get shredded dried bean curd melon flower, grind to form powdery, in first beaker behind the adding autoclaving, stirring and evenly mixing;
(c) multivita-glucose, pollen and honey are added in second beaker, add the distilled water after sterilizing then, fully mixing obtains first mixed liquor;
(d) first mixed liquor among the step c is poured in first beaker, with second beaker of distilled water flushing of sterilization, the distilled water of flushing usefulness is also poured in first beaker then, mix and obtain second mixed liquor;
(e) agar powder is added in the 3rd beaker, add and to be heated to behind the distilled water mixing after agar dissolves fully, stop heating, when treating that solution is cooled to 65-70 ℃, to wherein adding sorbic acid, stirring and dissolving obtains the 3rd mixed liquor;
(f) add in second mixed liquor after the 3rd mixed liquor is cooled to 55-60 ℃, stir and obtain product, be stored in 4 ℃ the refrigerator standby.
Adopt the eye spot blister beetle adult man-made feeds of technique scheme preparation, can satisfy a spot blister beetle adult nutritional requirement, under 4 ℃ of conditions, can preserve more than 1 month.
In technique scheme: the autoclaving condition in the step (a) is 121 ℃ of temperature, pressure 1.03 * 10
5-1.24 * 10
5High pressure steam sterilization 20～40min under the Pa.
Beneficial effect: raw material of the present invention are easy to get, and cost is low, and can satisfy the needs of the growth of eye spot blister beetle adult, growth and mate and oviposit, have solved the problem that eye spot blister beetle adult needs fresh flower to live.
The specific embodiment
Further the present invention is illustrated below in conjunction with embodiment.
Shredded dried bean curd melon flower among the present invention can not influence the nutritional labeling of sponge gourd flower like this for male sponge gourd flower petal and the pistil of newly taking obtains through after the freeze-drying.
Contain Cobastab in the multivita-glucose that uses among the present invention
1, Cobastab
2, vitamin C
2, vitamin D
2, nicotinic acid and glucose.
Used pollen is outsourcing pollen (pollen is the sporidiole of flowering plant) in the present invention's prescription, and the kind of spending is not required, and can be the pollen of various flowers.
Embodiment 1
Feed is made up of each component of following percentage by weight: analysis for soybean powder 10.7%, dusty yeast 6.4%, shredded dried bean curd melon spend 7.2%, multivita-glucose 5.4%, pollen 2.2%, honey 2.2%, water 64%, agar 1.4% and sorbic acid 0.22%.
The method for preparing above-mentioned feed is:
Take by weighing 15g analysis for soybean powder and 9g dusty yeast, be placed in first beaker, seal the back at 121 ℃, 1.03 * 10 with gauze
5～1.24 * 10
5Pa autoclave sterilization 30min.It is cooled to 40 ℃ then, adds the powdered shredded dried bean curd melon flower of 10g, in time stirring and evenly mixing prevents that mixture unity is on wall of cup.
Other gets second beaker, adds 3g honey, 3g pollen and 7.5g multivita-glucose, and stirring with the distilled water 20ml of about the 40 ℃ bacterium of going out obtains first mixed liquor.
First mixed liquor in second beaker is poured in first beaker,, the distilled water of flushing also added to stir in first beaker in the lump obtain second mixed liquor with went out second beaker of distilled water flushing of bacterium of 10ml.
Get the 3rd beaker again, add the 2g agar powder, be heated to agar behind the 60ml distilled water mixing and dissolve fully and stop heating, this moment, the temperature of solution was about 100 ℃, treated to add the 0.3g sorbic acid when solution is cooled to 65 ℃, and stirring and dissolving gets the 3rd mixed liquor.(this moment, agar solution did not also solidify) poured the 3rd mixed liquor in second mixed solution into when grade in an imperial examination three mixed liquors were cooled to 60 ℃, mixed rapidly, obtained product after the cooling.Encapsulate in back 4 ℃ of refrigerators preserve standby.
Eye spot blister beetle adult forage according to the preparation of this preferred embodiment is a kind of yellowish-brown pasty masses, and soya bean fragrance is arranged.
Embodiment 2
Feed is made up of each component of following percentage by weight: analysis for soybean powder 7%, dusty yeast 8%, shredded dried bean curd melon spend 4%, multivita-glucose 5.5%, pollen 2%, honey 1%, water 70%, agar 2% and sorbic acid 0.5%.
The method for preparing above-mentioned feed is:
Take by weighing 11g analysis for soybean powder and 12.6g dusty yeast, be placed in first beaker, seal the back at 121 ℃, 1.03 * 105～1.24 * 105Pa autoclave sterilization 20min with gauze.It is cooled to 50 ℃ then, adds the powdered shredded dried bean curd melon flower of 6.3g, timely stirring and evenly mixing, and prevent that mixture unity is on wall of cup.
Other gets second beaker, adds 1.6g honey, 3.2g pollen and 8.7g multivita-glucose, and stirring with the distilled water 25ml of about the 40 ℃ bacterium of going out obtains first mixed liquor.
First mixed liquor in second beaker is poured in first beaker,, the distilled water of flushing also added to stir in first beaker in the lump obtain second mixed liquor with went out second beaker of distilled water flushing of bacterium of 10ml.
Get the 3rd beaker again, be heated to agar powder behind adding 3.0g agar powder, the 70ml distilled water mixing and dissolve fully, this moment, the temperature of solution was about 100 ℃, stopped heating, treated to add the 0.8g sorbic acid when solution is cooled to 70 ℃, and stirring and dissolving gets the 3rd mixed liquor.(this moment, agar solution did not solidify) poured the 3rd mixed liquor in second mixed liquor into when grade in an imperial examination three mixed liquors were cooled to 55 ℃, mixed, and obtained product.Sealing is preserved standby in 4 ℃ of refrigerators.
Eye spot blister beetle adult forage according to the preparation of this preferred embodiment is a kind of yellowish-brown pasty masses, and soya bean and yeast fragrance are arranged.
Embodiment 3
Feed is made up of each component of following percentage by weight: analysis for soybean powder 15%, dusty yeast 5.9%, shredded dried bean curd melon spend 10%, multivita-glucose 3%, pollen 1%, honey 3%, water 60%, agar 2% and sorbic acid 0.1%.
The method for preparing above-mentioned feed is:
Take by weighing 21g analysis for soybean powder and 8.3g dusty yeast, be placed in first beaker, seal the back at 121 ℃, 1.03 * 105～1.24 * 105Pa autoclave sterilization 40min with gauze.It is cooled to 60 ℃ then, adds the powdered dried male sponge gourd flower of 14g, in time stirring and evenly mixing prevents that mixture unity is on wall of cup.
Other gets second beaker, adds 4.2g honey, and 1.4g pollen and 4.2g multivita-glucose obtain first mixed liquor with the distilled water 20ml stirring and dissolving of about the 40 ℃ bacterium of going out.
First mixed liquor in second beaker is poured in first beaker,, the distilled water of flushing also added to stir in first beaker in the lump obtain second mixed liquor with went out second beaker of distilled water flushing of bacterium of 9ml.
Get the 3rd beaker again, add the 2.8g agar powder, 55ml distilled water is heated to agar powder and dissolves fully, and this moment, the temperature of solution was about 100 ℃, stopped heating, treats to add the 0.14g sorbic acid when solution is cooled to 68 ℃, and stirring and dissolving gets the 3rd mixed liquor.Grade in an imperial examination three mixed liquors are cooled to 58 ℃ (this moment, agar solution did not solidify), and the 3rd mixed solution is poured in second mixed liquor, mix, and obtain product, and sealing is preserved standby in 4 ℃ of refrigerators.
Eye spot blister beetle adult forage according to the preparation of this preferred embodiment is a kind of yellowish-brown pasty masses, and soya bean fragrance is arranged.
Claims (4)
1. eye spot blister beetle adult man-made feeds is characterized in that being made up of each component of following percentage by weight: analysis for soybean powder 7%～15%, dusty yeast 3%～10%, shredded dried bean curd melon spend 4%～10%, multivita-glucose 3%～7%, pollen 1%～3%, honey 1%～3%, water 60%～70%, agar 1%～2% and sorbic acid 0.1%～0.5%.
2. according to described spot blister beetle of claim 1 adult man-made feeds, it is characterized in that: described shredded dried bean curd melon flower obtains through after the freeze-drying for male sponge gourd flower petal and the pistil of newly taking.
3. method for preparing eye as claimed in claim 1 spot blister beetle adult man-made feeds is characterized in that: preparation as follows:
(a) get analysis for soybean powder and dusty yeast and pack into and mix in first beaker, seal laggard horizontal high voltage sterilization;
(b) get shredded dried bean curd melon flower, grind to form powdery, in first beaker behind the adding autoclaving, stirring and evenly mixing;
(c) multivita-glucose, pollen and honey are added in second beaker, add the distilled water after sterilizing then, fully mixing obtains first mixed liquor;
(d) first mixed liquor among the step c is poured in first beaker, with second beaker of distilled water flushing of sterilization, the distilled water of flushing usefulness is also poured in first beaker then, mix and obtain second mixed liquor;
(e) agar powder is added in the 3rd beaker, add distilled water and be heated to after agar dissolves fully, stop heating, when treating that solution is cooled to 65-70 ℃, to wherein adding sorbic acid, stirring and dissolving obtains the 3rd mixed liquor;
(f) add in second mixed liquor after the 3rd mixed liquor is cooled to 55-60 ℃, stir and obtain product, product is stored in 4 ℃ the refrigerator standby.
4. according to the described method for preparing eye spot blister beetle adult man-made feeds of claim 3, it is characterized in that: the autoclaving condition in the step (a) is 121 ℃ of temperature, pressure 1.03 * 10
5-1.24 * 10
5High pressure steam sterilization 20～40min under the Pa.
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Cited By (10)
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| CN104187195A (en) * | 2014-09-26 | 2014-12-10 | 张可池 | Additive for mylabris phalerata pallas adult artificial feed |
| CN105707491A (en) * | 2016-03-17 | 2016-06-29 | 四川汪氏动物保健有限责任公司 | Nutritional cream for pet dogs or cats and preparation method thereof |
| CN107484929A (en) * | 2017-09-30 | 2017-12-19 | 孝感市鲁建农业开发有限公司 | A kind of breeding feed for improving Chinese blister beetle quality and preparation method thereof |
| CN107594111A (en) * | 2017-09-15 | 2018-01-19 | 孝感市鲁建农业开发有限公司 | A kind of Chinese blister beetle breeding feed and preparation method thereof |
| CN107751661A (en) * | 2017-10-18 | 2018-03-06 | 孝感市鲁建农业开发有限公司 | A kind of bionical feed for Chinese blister beetle cultivation and preparation method thereof |
| CN107801876A (en) * | 2017-12-11 | 2018-03-16 | 甘孜藏族自治州农业科学研究所 | A kind of man-made feeds of Chinese blister beetle adult and its preparation method and application |
| CN107969576A (en) * | 2017-12-11 | 2018-05-01 | 四川省农业科学院植物保护研究所 | A kind of man-made feeds of Larva of Mylabris phalerata and its preparation method and application |
| CN108244060A (en) * | 2018-04-24 | 2018-07-06 | 四川省农业科学院植物保护研究所 | A kind of mating system of the black mylabris cichorii of Huang |
| WO2020062979A1 (en) * | 2018-09-28 | 2020-04-02 | 广东省生物资源应用研究所 | Artificial diet for small hive beetle |
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2011
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| CN104187195A (en) * | 2014-09-26 | 2014-12-10 | 张可池 | Additive for mylabris phalerata pallas adult artificial feed |
| CN104187195B (en) * | 2014-09-26 | 2016-06-15 | 张可池 | A kind of additive of Mylabris phalarata adult man-made feeds |
| CN105707491A (en) * | 2016-03-17 | 2016-06-29 | 四川汪氏动物保健有限责任公司 | Nutritional cream for pet dogs or cats and preparation method thereof |
| CN107594111A (en) * | 2017-09-15 | 2018-01-19 | 孝感市鲁建农业开发有限公司 | A kind of Chinese blister beetle breeding feed and preparation method thereof |
| CN107484929A (en) * | 2017-09-30 | 2017-12-19 | 孝感市鲁建农业开发有限公司 | A kind of breeding feed for improving Chinese blister beetle quality and preparation method thereof |
| CN107751661A (en) * | 2017-10-18 | 2018-03-06 | 孝感市鲁建农业开发有限公司 | A kind of bionical feed for Chinese blister beetle cultivation and preparation method thereof |
| CN107801876A (en) * | 2017-12-11 | 2018-03-16 | 甘孜藏族自治州农业科学研究所 | A kind of man-made feeds of Chinese blister beetle adult and its preparation method and application |
| CN107969576A (en) * | 2017-12-11 | 2018-05-01 | 四川省农业科学院植物保护研究所 | A kind of man-made feeds of Larva of Mylabris phalerata and its preparation method and application |
| CN107969576B (en) * | 2017-12-11 | 2021-01-19 | 四川省农业科学院植物保护研究所 | Artificial feed for cantharis larvae, and preparation method and application thereof |
| CN108244060A (en) * | 2018-04-24 | 2018-07-06 | 四川省农业科学院植物保护研究所 | A kind of mating system of the black mylabris cichorii of Huang |
| CN108244060B (en) * | 2018-04-24 | 2020-03-24 | 四川省农业科学院植物保护研究所 | Breeding method of yellow black mylabris |
| WO2020062979A1 (en) * | 2018-09-28 | 2020-04-02 | 广东省生物资源应用研究所 | Artificial diet for small hive beetle |
| CN116349805A (en) * | 2023-05-04 | 2023-06-30 | 贵州大学 | Pegyne flatus-genkwa adult feed and preparation method thereof |
| CN116349805B (en) * | 2023-05-04 | 2024-03-19 | 贵州大学 | Pegyne flatus-genkwa adult feed and preparation method thereof |
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Application publication date: 20110914 |