CN102669486A - Special animal biogen for shrimps and preparation method thereof - Google Patents
Special animal biogen for shrimps and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a special animal biogen for shrimps and a preparation method thereof. The special animal biogen comprises raw materials of feed-grade fulvic acid, total flavones from taraxaci herba and a bitter soybean meal fermentation culture, wherein the bitter soybean meal fermentation culture comprises bitter soybean meal substrates and inoculums; the bitter soybean meal substrates comprise bitter soybean meal, maize meal and beet molasses; the inoculums comprise saccharomyces cerevisiae, lactobacillus plantarum, bacillus subtillis and live bacillus licheniformis which have total sum of the number of 1*10<8> to 10*10<8>cfu/g; the total sum of the number of bacillus subtillis and live bacillus licheniformis is 3*10<8> to 8*10<8>; the number of the saccharomyces cerevisiae is 0.5*10<8> to 3*10<8>; and the number of the lactobacillus plantarum is 0.5*10<8> to 2*10<8>. With the adoption of the special animal biogen for the shrimps, the technical defects of serious drug resistance, reduction of immunity, endogenous infection, double infection, harm for human health and the like caused by unreasonable use of large amount of antibiotics in the prior art are effectively overcome, and the effects of promoting growth, improving immunity, preventing and resisting diseases and improving animal product quality are achieved by repairing, regulating and exciting a digestive system and an immune system of an animal. The special animal biogen for the shrimps is a natural green product free of hormone.
Description
Technical field
The present invention relates to a kind of animal life element and preparation method thereof, particularly relate to seed shrimp tailored version animal life element and preparation method thereof.
Background technology
Along with the fast development of shrimp aquaculture industry and improving constantly of intensive culture degree, the disease problem is serious day by day.In traditional pest control method, antibiotic and chemicals are used as main means and use with a large amount of, have caused pathogen to develop immunity to drugs, and result of treatment descends; While has a strong impact on the health and the Environmental security of consumer groups owing to medicine is residual.Because the aquatic products medicament residue exceeds standard, and causes product limited, has had a strong impact on the competitiveness of China's aquatic products, has hindered the developing of domestic and international market, has caused enormous economic loss.Along with restriction and the forbidding of antibiotic in feed, green.Non-nutritive feed nuisanceless, environmental protection more and more receives publicity.
Since the forties in last century, antibiotic came out, antibiotic was the essential drugs of control shrimp diarrhoea always, and in one period, has brought into play indelible magical effect.But in the antibiotic large-scale application, also there is following major defect in it:
(1) resistance to the action of a drug is serious.A large amount of irrational use antibiotic not only cause antibody-resistant bacterium quantity to increase, but also multiple drug resistance strain occurred.
(2) immunity degradation.A large amount of antibiotic are ingested behind the body; Can be distributed to each histoorgan such as lymph node, kidney,liver,spleen, thymus gland, lung and bone with blood circulation; Antibiotic can cause antigenic quality to reduce; Directly influence immunologic process, thereby the inoculation of vaccine is produced harmful effect, the immunocompetence of animal body also can be weakened gradually.
(3) autogenous infection and suprainfection.Antibiotic is difficult to avoid can influence in the body profitable strain growth simultaneously acting on pathogen; Especially long-term a large amount of antibiotic that uses can cause flora imbalance in the body, and microecological balance destroys; The harmful bacterium that hides in vivo takes advantage of the occasion to breed in a large number, and causes endogenous infection; Antibiotic can be eliminated sensitive bacteria in the body, causes a large amount of rooms on the certain micro-organisms attachment point in vivo, and taking advantage of a weak point for extraneous resistance germ offers an opportunity, thereby causes external source to infect.Suprainfection also is owing to when using a large amount of antibiotic to kill certain bacterium, destroy microecological balance, and one or more are endogenous in addition or the external source germ infects once more immediately that body causes.
(4) jeopardize human health.Antibiotic is distributed to almost each organ of whole body after being absorbed in the body, and most of antibiotics is excreted by ight soil, also can remain in the milk through the lactation process.After being excreted in the environment, the antibiotic of some stable in properties still can have a very long time; Thereby cause the medicament residue in the environment; These remaining medicines are slowly accumulated in human body and other plant body through livestock products and environment, finally come together in human body with various approach, cause human body to produce a large amount of antibody-resistant bacterium; Lose resistance, or body is produced toxic action because of accumulating in a large number to some disease.
Summary of the invention
It is plain and preparation method thereof to the purpose of this invention is to provide a seed shrimp tailored version animal life, overcomes effectively that the resistance to the action of a drug that a large amount of unreasonable use antibiotic of prior art cause is serious, immunity degradation, autogenous infection and suprainfection, the technological deficiency such as human health that jeopardizes.
For realizing above-mentioned purpose; The invention provides seed shrimp tailored version animal life element and preparation method thereof, its preparation raw material comprises following constituent: feed grade fulvic acid 45%-60%, dandelion general flavone 0.2%-0.4%, bitter bean pulp fermentation culture 39.3-60%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 85-98%, corn flour: 1-7%, beet molasses 0.5-1.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 1 * 10
8-10 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 3 * 10
8-8 * 10
8Cfu/g, S. cervisiae 0.5 * 10
8-3 * 10
8Cfu/g, Lactobacillus plantarum 0.5 * 10
8-2 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 4-7: 3-5 (W/V).
It is one or both or three kinds in brown coal, weathered coal or the peat that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal, peat or one or both or three kinds are crushed to the 60-80 order, with the raw material after pulverizing by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in immersion 2-3 hour down of 60 ℃-80 ℃ of temperature;
With said extracted liquid after centrifugal slagging-off, by 100: it is 30% hydrogen peroxide solution that the part by weight of 1-2 adds mass concentration, in 70 ℃ of-80 ℃ of following activation 1.5-2 of temperature hours, obtains activating solution;
With molar concentration be hydrochloric acid solution and the mass concentration of 1mol/L be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants; With above-mentioned activating solution and said compound precipitants by 1: the volume ratio of 50-80 mixes; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid.
The bitter bean pulp fermentation culture of said preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled; Mix afterwards and descended anaerobic fermentation 38-60 hour more first aerobic fermentation 16-36 hour for 20 ℃-40 ℃ in temperature.
A seed shrimp tailored version animal life provided by the invention is plain and preparation method thereof, has the following advantages:
Lactobacillus plantarum can purify water: particularly cultures the middle and later periods, and too much organic, water quality such as Heisui River, Lao Shui, red water.Can decompose simultaneously organic matter at the bottom of the pool, algae toxin is eliminated in deodorizing, builds good habitat.Degraded water body ammonia nitrogen, harmful substances such as nitrite reduce organic oxygen demand, and oxygenation indirectly improves water quality.Can also keep the algae phase, bacterium is balanced each other, and reduces and stablizes water pH value.
Plant lactobacillus can provide available essential amino acid and various vitamin (vitamin B complex and K etc.) for the host; Also the biologically active of mineral element be can improve, and then Nutrition and Metabolism, the directly effect of short its growth that the host carries essential nutriment, enhancing animal are reached for.Bacillus acidi lactici is the obviously phagocytosis of activating macrophage on the one hand, on the other hand because it can be equivalent to natural active immunity in the enteron aisle field planting.They can also stimulate peritoneal macrophages, induce the generation interferon, promote cell division, produce antibody and promote cellular immunity etc., thus the non-specific and specific immune response of ability enhancing body, the resistance against diseases of raising body.The animal and bird intestines environment be slant acidity could guarantee that lactic acid bacteria can secrete organic acid by harmful bacterium invasion and attack, keep normal pH value in the enteron aisle; After adding lactic acid bacteria in addition; Can also promote gemma bacterium and saccharomycetic increment, make the beneficial bacterium status of having the advantage fast, help intestinal health.
The present invention has probio, multiple Chinese herbal medicine and other nutriment that can improve functions of intestines and stomach and improve the animal immune function to be composited through the science compatibility.Can reach growth promotion through digestive system, the immune system of repairing, regulating, exciting animal body self, improve immunity, disease resistance, improve the effect of animal product quality.These article are not for containing the natural green product of any hormone.
Through embodiment, technical scheme of the present invention is done further detailed description below.
The specific embodiment
Further specify the plain technical scheme of shrimp tailored version animal life of the present invention through specific embodiment below.
Embodiment 1
One seed shrimp tailored version animal life is plain and preparation method thereof, and its preparation raw material comprises following constituent: feed grade fulvic acid 50%, dandelion general flavone 0.3%, bitter bean pulp fermentation culture 49.7%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 95%, corn flour: 4%, beet molasses 1%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 8 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 6 * 10
8Cfu/g, S. cervisiae 1 * 10
8Cfu/g, Lactobacillus plantarum 1 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 6: 4 (W/V).
It is brown coal, weathered coal that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal are crushed to 70 orders, and it is in 2% the sodium hydroxide solution that the raw material after pulverizing is added mass concentration by 1: 9 part by weight, in the immersion 2.5 hours down of 70 ℃ of temperature;
After centrifugal slagging-off, adding mass concentrations by 100: 1 part by weight is 30% hydrogen peroxide solution, in 70 ℃ of following activation of temperature 2 hours, obtains activating solution with said extracted liquid;
With molar concentration is that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 2 volume ratio for hydrochloric acid solution and the mass concentration of 1mol/L; Above-mentioned activating solution is mixed with the volume ratio of said compound precipitants by 1: 65; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid of pH value=6.8.
The bitter bean pulp fermentation culture of said preparation is specially: composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 3: 10 ml/kg ratio, mixes the back in the first aerobic fermentations 25 hours down of 30 ℃ of temperature, anaerobic fermentation is 50 hours again.
Embodiment 2
One seed shrimp tailored version animal life is plain and preparation method thereof, and its preparation raw material comprises following constituent: feed grade fulvic acid 45%, dandelion general flavone 0.4%, bitter bean pulp fermentation culture 54.6%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 93%, corn flour: 5.5%, beet molasses 1.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 8.5 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 5 * 10
8Cfu/g, S. cervisiae 2 * 10
8Cfu/g, Lactobacillus plantarum 1.5 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 5: 5 (W/V).
It is brown coal, peat that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, peat dust are broken to 80 orders, and it is in 4% the sodium hydroxide solution that the raw material after pulverizing is added mass concentration by 1: 10 part by weight, in the immersion 3 hours down of 65 ℃ of temperature;
After centrifugal slagging-off, adding mass concentrations by 100: 2 part by weight is 30% hydrogen peroxide solution, in 75 ℃ of following activation of temperature 1.8 hours, obtains activating solution with said extracted liquid;
With molar concentration is that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 3 volume ratio for hydrochloric acid solution and the mass concentration of 1mol/L; Above-mentioned activating solution is mixed with the volume ratio of said compound precipitants by 1: 80; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain pH value=6.5 feed grade fulvic acids.
The bitter bean pulp fermentation culture of said preparation is specially: composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 2: 10 ml/kg ratio, mixes the back in the first aerobic fermentations 27 hours down of 20 ℃ of temperature, anaerobic fermentation is 45 hours again.
Embodiment 3
One seed shrimp tailored version animal life is plain and preparation method thereof, and its preparation raw material comprises following constituent: feed grade fulvic acid 58, dandelion general flavone 0.2%, bitter bean pulp fermentation culture 41.8%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 97%, corn flour: 2.5%, beet molasses 0.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 9 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 4 * 10
8Cfu/g, S. cervisiae 3 * 10
8Cfu/g, Lactobacillus plantarum 2 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 7: 3 (W/V).
It is brown coal, weathered coal, peat that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal, peat dust are broken to 60 orders, and it is in 3% the sodium hydroxide solution that the raw material after pulverizing is added mass concentration by 1: 8 part by weight, in the immersion 3 hours down of 60 ℃ of temperature;
After centrifugal slagging-off, adding mass concentrations by 100: 1.5 part by weight is 30% hydrogen peroxide solution, in 80 ℃ of following activation of temperature 1.5 hours, obtains activating solution with said extracted liquid;
With molar concentration is that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 1 volume ratio for hydrochloric acid solution and the mass concentration of 1mol/L; Above-mentioned activating solution is mixed with the volume ratio of said compound precipitants by 1: 70; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain pH value=7 feed grade fulvic acids.
The bitter bean pulp fermentation culture of said preparation is specially: composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 4: 10 ml/kg ratio, mixes the back in the first aerobic fermentations 32 hours down of 35 ℃ of temperature, anaerobic fermentation is 40 hours again.
In the technical scheme of plain embodiment 1 to 3 embodiment of the invention described above shrimp tailored version animal life, said bitter dregs of beans is the residue of Sophora alopecuroide after extracting matrine, available from Inner Mongol Huimin biotech firm.
Shrimp tailored version animal life element of the present invention can be admixed the shrimp of feeding in the feed according to the ratio of 3-5 kg/ton and get final product.
Technical scheme through the plain embodiment 1 to embodiment 3 of the invention described above shrimp tailored version animal life can be found out; Compared with prior art, shrimp tailored version animal life element of the present invention has the feeding environment of improvement and pond water quality, promotes that shrimp grows; Improve food conversion ratio; Enhancing body immunity, disease resistance, advantages such as additional income.
Through concrete test the remarkable efficacy that shrimp tailored version animal life of the present invention is plain is described below.
Choose the shrimp culture pond that 4 areas equate basically, 1 meter of mean depth is divided into the test combinations control group at random, puts the freshwater shrimp seedling that originates from same batch of same plant in a suitable place to breed through after the early-stage preparations, and breeding density is 1,800,000 tail/hectares, and specification is 8000 tail/kilograms.The control group daily ration of normally feeding, test group add the special-purpose animal life element of shrimp in 0.5% ratio and feed in daily ration.Feeding time is 4 months altogether when fishing for.The result is following:
Table 1 is respectively cultured the production performance of pond freshwater shrimp
It should be noted last that; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although the present invention is specified with reference to preferred embodiment; Those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention, and do not break away from the spirit and the scope of technical scheme of the present invention.
Claims (4)
1. shrimp tailored version animal life is plain and preparation method thereof, and it is characterized in that: its preparation raw material comprises following constituent: feed grade fulvic acid 45%-60%, dandelion general flavone 0.2%-0.4%, bitter bean pulp fermentation culture 39.3-60%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 85-98%, corn flour: 1-7%, beet molasses 0.5-1.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 1 * 10
8-10 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 3 * 10
8-8 * 10
8Cfu/g, S. cervisiae 0.5 * 10
8-3 * 10
8Cfu/g, Lactobacillus plantarum 0.5 * 10
8-2 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 4-7: 3-5 (W/V).
2. shrimp tailored version animal life according to claim 1 is plain and preparation method thereof, it is characterized in that: it is one or both or three kinds in brown coal, weathered coal or the peat that said fulvic acid extracts raw material.
3. shrimp tailored version animal life according to claim 1 is plain and preparation method thereof, and it is characterized in that: the extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal, peat or one or both or three kinds are crushed to the 60-80 order, with the raw material after pulverizing by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in immersion 2-3 hour down of 60 ℃-80 ℃ of temperature;
With said extracted liquid after centrifugal slagging-off, by 100: it is 30% hydrogen peroxide solution that the part by weight of 1-2 adds mass concentration, in 70 ℃ of-80 ℃ of following activation 1.5-2 of temperature hours, obtains activating solution;
With molar concentration be hydrochloric acid solution and the mass concentration of 1mol/L be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants; With above-mentioned activating solution and said compound precipitants by 1: the volume ratio of 50-80 mixes; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid.
4. shrimp tailored version animal life according to claim 1 is plain and preparation method thereof; It is characterized in that: the bitter bean pulp fermentation culture of said preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled; Mix afterwards and descended anaerobic fermentation 38-60 hour more first aerobic fermentation 16-36 hour for 20 ℃-40 ℃ in temperature.
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| CN104450582A (en) * | 2014-12-10 | 2015-03-25 | 北京世纪新探生物科技有限公司 | Lactobacillus plantarum, feed premix containing lactobacillus plantarum and preparation method of lactobacillus plantarum |
| CN108142642A (en) * | 2018-03-15 | 2018-06-12 | 山东碧蓝生物科技有限公司 | A kind of nutrition immune type prawn soft pellet diet and preparation method thereof |
| CN114223810A (en) * | 2021-12-13 | 2022-03-25 | 南京宝辉生物饲料有限公司 | Soft pellet feed for freshwater shrimp culture and preparation method thereof |
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| CN108142642A (en) * | 2018-03-15 | 2018-06-12 | 山东碧蓝生物科技有限公司 | A kind of nutrition immune type prawn soft pellet diet and preparation method thereof |
| CN114223810A (en) * | 2021-12-13 | 2022-03-25 | 南京宝辉生物饲料有限公司 | Soft pellet feed for freshwater shrimp culture and preparation method thereof |
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