CN101082026A

CN101082026A - Preparation of holothurian-nourishing bacterium and method for restoring sea cucumber cultivation pool environment

Info

Publication number: CN101082026A
Application number: CNA2006100271329A
Authority: CN
Inventors: 邹国忠
Original assignee: Shanghai Hongbao Green Aquatic Product Science & Technology Development Co Ltd
Current assignee: Shanghai Hongbao Green Aquatic Product Science & Technology Development Co Ltd
Priority date: 2006-05-31
Filing date: 2006-05-31
Publication date: 2007-12-05
Anticipated expiration: 2026-05-31
Also published as: CN101082026B

Abstract

The sea cucumber nourishing bacterial preparation is environment friendly composite live bacterial preparation developed based on the physiological and ecological features of sea cucumber and the characteristics of water in sea cucumber raising pond and by means of modern biotechnology and for nourishing and protecting sea cucumber. The sea cucumber nourishing bacterial preparation is prepared through sorting and culturing live bacteria, adding Chinese herbal medicine extract to the culture medium, liquid or solid fermenting, and adding protectant and Chinese herbal medicine supplementary material. After being thrown into sea cucumber raising pond, the dormant live bacteria are revived and disintegrated fast to decompose organic pollutant in water, eliminate harmful matter, inhibit pathogenic bacteria, balance the flora in sea cucumber's intestinal tract and promote sea cucumber's growth.

Description

Support preparation of ginseng bacterium and the method for repairing the holothruian cultures pool environment

Support the ginseng bacterium and be specially at sea cucumber physiology, growth characteristic and ginseng pond water quality characteristics, the environmental type composite bacteria of using the modern biotechnology exploitation is supported ginseng, is protected the ginseng product.By meticulous choosing training viable bacteria bacterial classification; according to the utilizability of specified strain to natural medium; Chinese herbal medicine extract is used as in the substratum of specified strain; cultivate through liquid state or solid state fermentation; and acquisition contains the active bacteria formulation of multiple prebiotic substance; add protective material and specific herbal medicine auxiliary material again, last composite forming.After foster ginseng bacterium is rendered to the ginseng pond, the dormancy viable bacteria can breed in very fast recovery, decompose excessive organic pollutant in the ginseng pond rapidly, transform and eliminate objectionable impurities in the water, the benefit materials of active bacteria formulation self and be dissolved in the herbal medicine particulate of water body can play the effect that suppresses sea cucumber pathogenic bacterium field planting in the ginseng pond and kill the sea cucumber virulence factor.Support the ginseng bacterium and also can regulate micro-ecological bacterial group balance in the Intestinum Stichopi japonici road, the promotion sea cucumber digests and assimilates feed, and appetite stimulator strengthens the sea cucumber immunologic function.In the ginseng pond of being everlasting, splash and support the ginseng bacterium or cooperate holothurian feed to throw something and feed, can obviously shorten sea cucumber hibernation and aestivation time, increase the effective growth time of sea cucumber relatively, promote that sea cucumber grows fast, shorten sea cucumber and form the cycle.It is without any side effects to aquatic animal, the mankind and environment to support the ginseng bacterium, and life-time service can not develop immunity to drugs, and is that high-density breeding improves sea cucumber vitality, repairs ginseng pond ecological environment, produces the essential product of green sea cucumber.

[effect]

1, shortens sea cucumber hibernation and aestivation time, promote the sea cucumber growth, shorten and form the cycle;

2, suppress ginseng pond pathogenic bacterium field planting and invasion and attack, kill the sea cucumber virulence factor;

3, have adjusting metabolism of fat, anti-hereditary material DNA variation improves ginseng body immunizing power, repairs the function of ginseng somatocyte damage;

4, have the Green Tea Extract damage, reduce lipid peroxide, suppress the necrosis of ginseng body internal organ, the function of maintenance ginseng body health;

5, have the secretion of the endogenous of promotion digestive ferment, improve sea cucumber appetite, strengthen the effect that feed is digested and assimilated.

[physicochemical character] tawny powder has sharp flavor.

[main component] photosynthetic bacterium, milk-acid bacteria, yeast, actinomycetes, thread fungus, alkaloid, biological enzyme, flavonoid, VITAMIN, trace element, carbohydrate.

[active constituent content] every gram contains probiotic bacterium 20 * 10 ⁸Individual.

[use range] is applicable to all sea cucumber nursery ponds and growing pond (comprise and raise together with the pond), be specially adapted to culture sea cucumber for many years, mud is more, " gonorrhoea water ", " red water ", " black smelly water ", " old clear water " appear, and cause that sea cucumber is off one's feed, malnutrition, unusual, the slow growing aging pond of body colour.

[usage] evenly splashes with full pond, 10-20 times of Chi Shui dilution back or mixed feed is thrown something and fed.

[consumption]

1, maintenance sea cucumber: use every mu of water surface (1 meter of the depth of water) first with supporting ginseng bacterium 250-500 gram; Use every mu of water surface (1 meter of the depth of water) with supporting ginseng bacterium 100 grams later on, splashed once multiplicable splashing during water quality deterioration every 5-7 days.

2, grow seedlings: every cubic metre of water body 5 grams of splashing, splashed once every 2-3 days.

3, mixed feed uses: every double centner feed uses 500 grams.

[attention]

1, throws in " supporting the ginseng bacterium " bans use of germicidal action in first three day, back five days pharmaceutical chemicals and antibiotic medicine;

2, use foster ginseng bacterium not to be subjected to the influence of weather, water temperature, often use can guarantee that water quality is good, promotes growth;

3, foster ginseng bacterium is safe in utilization, and multiplicable use when water quality is aging serious is used and can be changed water after 3 days.

[summary of the invention]

Probiotics of the present invention and preparation method thereof, as follows:

A, get PS positively charged ion gel starch and be dissolved in water, dilute 10 times, sterilize after being made into 10% solution.

B, the A solution after will dissolving fully add in the cultured EM bacterium bacterium liquid by the 1-3% addition, stirred several minutes, treat to have in the bacterium liquid thalline throw out to produce, after supernatant liquor and the concentrated throw out solid-liquid separation, go to collect somatic cells after the most of moisture with the nylon filter cloth.

C, get B ultrasonic and concentrate EM bacterium somatic cells and add in the dry powder ooze, stirred several minutes by the 5-10% addition.

1. D, 60 mesh sieves select the powder kuh-seng, standby.2. 60 mesh sieves select the powdery ginger, standby.3. 60 mesh sieves select the powdery Radix Astragali, standby.

E, liquid genus bacillus, photosynthetic bacterium, milk-acid bacteria, yeast, actinomycetes mixed solution.

F, 60 mesh sieves select the powder rice bran, and are standby.

G, get that D is standby 1., standby 2., standby 3., E, F, brown sugar and water, in 10: 25: 10: 2: 27: 1: the composite mixing and stirring of 25 ratios.

H, pour G into fermentation hole, sealing and fermenting 10-15 days, oven dry was pulverized then, made herbal medicine composite bacteria auxiliary material through the choosing of 80 mesh sieves.

I, with C gained bacterium ooze and H gained herbal medicine composite bacteria auxiliary material in 6: 4 composite mixing of ratio.

The present invention has strong operability, with low cost, effect is remarkable, to the water surrounding non-secondary pollution, sea cucumber is had no side effect, the holothruian cultures bottom environment there are advantages such as obvious repairing effect, be to comply with current social back to nature, treasure water and life, pursue green food, the development healthy aquaculture, the only way of realizing healthy consumption.

Claims

Support the ginseng bacterium and be specially at sea cucumber physiology, growth characteristic and ginseng pond water quality characteristics, the environmental type composite bacteria of using the modern biotechnology exploitation is supported ginseng, is protected the ginseng product.By meticulous choosing training viable bacteria bacterial classification; according to the utilizability of specified strain to natural medium; Chinese herbal medicine extract is used as in the substratum of specified strain; cultivate through liquid state or solid state fermentation; and acquisition contains the active bacteria formulation of multiple prebiotic substance; add protective material and specific herbal medicine auxiliary material again, last composite forming.After rendering to the ginseng pond, the dormancy viable bacteria can breed in very fast recovery, decompose excessive organic pollutant in the ginseng pond rapidly, transform and eliminate objectionable impurities in the water, the benefit materials of active bacteria formulation self and be dissolved in the herbal medicine particulate of water body can play the effect that suppresses sea cucumber pathogenic bacterium field planting in the ginseng pond and kill the sea cucumber virulence factor.Support the ginseng bacterium and also can regulate micro-ecological bacterial group balance in the Intestinum Stichopi japonici road, the promotion sea cucumber digests and assimilates feed, and appetite stimulator strengthens the sea cucumber immunologic function.

[summary of the invention]

Probiotics of the present invention and preparation method thereof, as follows:

A, get PS positively charged ion gel starch and be dissolved in water, dilute 10 times, sterilize after being made into 10% solution.

B, the A solution after will dissolving fully add in the cultured EM bacterium bacterium liquid by the 1-3% addition, stirred several minutes, treat to have in the bacterium liquid thalline throw out to produce, after supernatant liquor and the concentrated throw out solid-liquid separation, go to collect somatic cells after the most of moisture with the nylon filter cloth.

C, get B ultrasonic and concentrate EM bacterium somatic cells and add in the dry powder ooze, stirred several minutes by the 5-10% addition.

1. D, 60 mesh sieves select the powder kuh-seng, standby.2. 60 mesh sieves select the powdery ginger, standby.3. 60 mesh sieves select the powdery Radix Astragali, standby.

E, liquid genus bacillus, photosynthetic bacterium, milk-acid bacteria, yeast, actinomycetes mixed solution.

F, 60 mesh sieves select the powder rice bran, and are standby.

G, get that D is standby 1., standby 2., standby 3., E, F, brown sugar and water, in 10: 25: 10: 2: 27: 1: the composite mixing and stirring of 25 ratios.

H, pour G into fermentation hole, sealing and fermenting 10-15 days, oven dry was pulverized then, made herbal medicine composite bacteria auxiliary material through the choosing of 80 mesh sieves.

I, with C gained bacterium ooze and H gained herbal medicine composite bacteria auxiliary material in 6: 4 composite mixing of ratio.