CN101891307A - Marine culture biological cleanser and preparation method thereof - Google Patents
Marine culture biological cleanser and preparation method thereof Download PDFInfo
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- CN101891307A CN101891307A CN 201010227191 CN201010227191A CN101891307A CN 101891307 A CN101891307 A CN 101891307A CN 201010227191 CN201010227191 CN 201010227191 CN 201010227191 A CN201010227191 A CN 201010227191A CN 101891307 A CN101891307 A CN 101891307A
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Abstract
The invention discloses a marine culture biological cleanser and a preparation method thereof. The marine culture biological cleanser comprises the following components in percentage by mass: 5-18 ppm of chitosan oligosaccharide, 50-70% of sea water, 6-12% of biological bacterium solution, 6-12% of sucrose and 15-26% kelp dreg. The biological bacterium solution is a microbial preparation which is compounded and cultured from photosynthetic bacteria, bacillus and lactic acid bacteria. The chitosan oligosaccharide is prepared by the following steps: extracting chitin from Alaska queen crab, deacetylating the chitin to prepare chitosan, and carrying out enzyme digestion on the chitosan to obtain low-molecular weight chitosan. The invention saves the fresh water resources, lowers the cost and enhances the purifying effect for marine culture.
Description
Technical field
The present invention relates to the scavenging agent field, especially a kind of marine culture biological cleanser and preparation method thereof.
Background technology
At present, along with Economic development and people's living standard improve, fishery products more and more enter into huge numbers of families, and in order to satisfy people's demand, corresponding aquaculture scale is also increasing, causes water to pollute and is on the rise.And be used for the scavenging agent that sea farming purifies now, mostly be to come the mixing match fermentation to form according to 7: 1: 2 ratio with fresh water, biological bacteria, nutritive substance, its effect is remarkable, long action time, and be pure biotechnological formulation, noresidue, but shortcoming is to expend a large amount of fresh water, and the effect of administering sea pollution is remarkable not as administering the fresh water pollution effect, the fresh water nutritive ingredient is less simultaneously, especially it is less to contain calcium ions and magnesium ions, can not supply with the required nutrition of the normal breeding of biological bacteria, must add a certain amount of carbon, nitrogen in proportion; And the deposit of fresh water has certain limit on the earth, and seawater converts that fresh water not only needs complicated technology to but also the cost that needs great number.
Summary of the invention
The technology of the present invention task is at the deficiency in the above-mentioned technology, and a kind of marine culture biological cleanser and preparation method thereof is provided.
Technical solution technical scheme that problem adopts of the present invention is: marine culture biological cleanser, the moiety of described marine culture biological cleanser and shared mass percent are: the oligochitosan of 5ppm-18ppm, the seawater of 50%-70%, the biological bacteria liquid of 6%-12%, the sucrose of 6%--12% and the kelp residue of 15%-26%, wherein said biological bacteria liquid is photosynthetic bacteria, the compound cultured microorganism preparation of genus bacillus and milk-acid bacteria, described oligochitosan is to be raw material with Alaska snow crab, chitin extraction therefrom, be prepared into chitosan by chitin through deacetylation, again chitosan be reduced to low-molecular-weight oligochitosan through enzymolysis;
Described photosynthetic bacteria is a Rhodopseudomonas palustris, and Rhodopseudomonas palustris nutrient solution prescription is: potassium primary phosphate 0.3-0.6 gram, sal epsom 0.3-0.6 gram, sodium acetate 2.0-5.0 gram, Yeast diffusion juice 1.0-2.5 gram, sterilization seawater 600-1200 milliliter.
Described genus bacillus nutrient solution prescription is: peptone 3.0-6.0 gram, extractum carnis 2.0-4.0 gram, distilled water 500ml-800ml, agar 15.0-22.0 gram.
Described milk-acid bacteria prescription is: be to add peptone 7.0-12.0 gram, extractum carnis 7.0-12.0 gram, yeast extract paste 3.0-6.0 gram, diammonium hydrogen citrate 1.0-3.5 gram, glucose 15.0-26.0 gram, tween 700.0-900.0 gram, sodium acetate 3.0-6.0 gram, dipotassium hydrogen phosphate 1.0-3.5 gram, sal epsom 0.3-0.8 gram, manganous sulfate 0.15-0.30 gram, agar 12.0-23.0 gram, distilled water 700-1200ml in the environment of 5.5-7.5 at pH.
Marine culture biological cleanser, its preparation method is as follows: at first be through sterilization and disinfection, immersion, pulverizing, grinding and slurrying with kelp residue, obtain the marine alga slurry, with this marine alga slurry and biological bacteria liquid, oligochitosan, seawater and the sucrose thorough mixing, stirring, the fermentation that have prepared, finally become finished product then then.
The beneficial effect that the present invention can bring is:
(1) well saves Freshwater resources, reduced cost;
(2) improved the decontamination effect improving of sea farming.
Embodiment
The moiety of described marine culture biological cleanser and shared mass percent are: the biological bacteria liquid of the oligochitosan of 5ppm-18ppm, the seawater of 50%-70%, 6%-12%, the sucrose of 6%--12% and the kelp residue of 15%-26%; wherein said biological bacteria liquid is the compound cultured microorganism preparation of photosynthetic bacteria, genus bacillus and milk-acid bacteria; described oligochitosan is to be raw material with Alaska snow crab; chitin extraction therefrom; be prepared into chitosan by chitin through deacetylation, again chitosan be reduced to low-molecular-weight oligochitosan through enzymolysis.Alaska snow crab that lives in the deep-sea, marine site, the Arctic Ocean is natural marine organisms, nontoxic, in leaching process, do not add any toxic substance, so the oligochitosan of producing is the marine bioactivity material of natural green, nontoxic, harmless, have no side effect, belong to the made from pure-natural marine living-things goods.The oligochitosan molecular weight is between 300～2000 dalton, and molecular weight is little, can be water-soluble, contain a plurality of hydroxyls and amino active group in the structural formula, have special physiological properties and special biological activity, can restrain growing of harmful germ, the propagation probiotics, the immunizing power of raising body.
Described photosynthetic bacteria is a Rhodopseudomonas palustris, and Rhodopseudomonas palustris nutrient solution prescription is: potassium primary phosphate 0.3-0.6 gram, sal epsom 0.3-0.6 gram, sodium acetate 2.0-5.0 gram, Yeast diffusion juice 1.0-2.5 gram, sterilization seawater 600-1200 milliliter.Because photosynthetic bacteria has the metabolic type that changes flexibly along with the variation of growth conditions, so it can handle the organic waste water of up to ten thousand milliliters of BOD concentration.Generally, the BOD5 clearance can reach 95%, even reaches 98%.Rich in proteins (65%) and vitamin B group in the photosynthetic bacteria cell have great practical value simultaneously, can be used as animal and fowl fodder and fishes and shrimps ear material etc.
Described genus bacillus nutrient solution prescription is: peptone 3.0-6.0 gram, extractum carnis 2.0-4.0 gram, distilled water 500ml-800ml, agar 15.0-22.0 gram.Genus bacillus is the section of bacterium, can form the bacillus or the coccus of gemma.Comprise bacillus, Sporolactobacillus, fusobacterium, Desulfotomaculum and gemma Sarcina etc.The injurious factor resistibility is strong to external world for they, and it is wide to distribute, and is present in soil, water, air and animal intestinal etc. and locates.For aerobic or amphimicrobian bacillus, be generally Gram-positive.Under certain environment, endobacillary structure changes, and through the prespore stage, forms a complete gemma.Gemma has very strong resistibility to heat, radioactive rays and chemical substance etc.The subtilyne that produces in the genus bacillus thalli growth process, polymyxin, nystatin, linear gramicidins isoreactivity material, these active substances have the obvious suppression effect to the conditioned pathogen of pathogenic bacterium or autogenous infection; Stimulate growing of animal immune organ, activate T, bone-marrow-derived lymphocyte, improve immunoglobulin (Ig) and antibody horizontal, strengthen cellular immunization and humoral immune function, improve herd immunity; Self synthesizes enzymes such as α-Dian Fenmei, proteolytic enzyme, lipase, cellulase, together plays a role with the intravital digestive enzymes of animal in digestive tube; Multiple vitamin B group such as synthesise vitamins B1, B2, B6, nicotinic acid, the activity of raising animal body internal interference element and scavenger cell
Described milk-acid bacteria prescription is: be to add peptone 7.0-12.0 gram, extractum carnis 7.0-12.0 gram, yeast extract paste 3.0-6.0 gram, diammonium hydrogen citrate 1.0-3.5 gram, glucose 15.0-26.0 gram, tween 700.0-900.0 gram, sodium acetate 3.0-6.0 gram, dipotassium hydrogen phosphate 1.0-3.5 gram, sal epsom 0.3-0.8 gram, manganous sulfate 0.15-0.30 gram, agar 12.0-23.0 gram, distilled water 700-1200ml in the environment of 5.5-7.5 at pH.Milk-acid bacteria can make uncorrupted organic matter fermentation, is converted into available nutrient, and to produce the thing carbohydrate with photosynthetic bacterium be matrix, produces lactic acid.Lactic acid has very strong sterilizing ability, particularly can suppress the harmful microbe breeding, and organic rapid corrupt the decomposition.The organism that milk-acid bacteria can make difficulties such as xylogen, Mierocrystalline cellulose decompose decomposes easily, and eliminates all drawbacks that undecomposed organism produces, and therefore can play a significant role aspect the organic matter fermentation decomposition.
Marine culture biological cleanser, its preparation method is as follows: at first be through sterilization and disinfection, immersion, pulverizing, grinding and slurrying with kelp residue, obtain the marine alga slurry, with this marine alga slurry and biological bacteria liquid, oligochitosan, seawater and the sucrose thorough mixing, stirring, the fermentation that have prepared, finally become finished product then then.
The present invention utilizes seawater to replace fresh water to cultivate biological cleanser and administers the sea farming pollution, better than fresh water cultivation biological cleanser improvement sea farming pollution effects, seawater cultivation biological cleanser improvement sea farming pollution effects is more remarkable, save the Freshwater resources of preciousness on the earth simultaneously, reduced production cost.
Claims (5)
1. marine culture biological cleanser; it is characterized in that: the moiety of described marine culture biological cleanser and shared mass percent are: the oligochitosan of 5ppm-18ppm; the seawater of 50%-70%; the biological bacteria liquid of 6%-12%; the sucrose of 6%--12% and the kelp residue of 15%-26%; wherein said biological bacteria liquid is photosynthetic bacteria; the compound cultured microorganism preparation of genus bacillus and milk-acid bacteria; described oligochitosan is to be raw material with Alaska snow crab; chitin extraction therefrom; be prepared into chitosan by chitin through deacetylation then, again chitosan finally be reduced to low-molecular-weight oligochitosan through enzymolysis.
2. marine culture biological cleanser according to claim 1, it is characterized in that: described photosynthetic bacteria is a Rhodopseudomonas palustris, and Rhodopseudomonas palustris nutrient solution prescription is: potassium primary phosphate 0.3-0.6 gram, sal epsom 0.3-0.6 gram, sodium acetate 2.0-5.0 gram, Yeast diffusion juice 1.0-2.5 gram, sterilization seawater 600-1200 milliliter.
3. marine culture biological cleanser according to claim 1 is characterized in that: described genus bacillus nutrient solution prescription is: peptone 3.0-6.0 gram, extractum carnis 2.0-4.0 gram, distilled water 500ml-800ml, agar 15.0-22.0 gram.
4. marine culture biological cleanser according to claim 1 is characterized in that: described milk-acid bacteria prescription is: be to add peptone 7.0-12.0 gram, extractum carnis 7.0-12.0 gram, yeast extract paste 3.0-6.0 gram, diammonium hydrogen citrate 1.0-3.5 gram, glucose 15.0-26.0 gram, tween 700.0-900.0 gram, sodium acetate 3.0-6.0 gram, dipotassium hydrogen phosphate 1.0-3.5 gram, sal epsom 0.3-0.8 gram, manganous sulfate 0.15-0.30 gram, agar 12.0-23.0 gram, distilled water 700-1200ml in the environment of 5.5-7.5 at pH.
5. marine culture biological cleanser according to claim 1, its preparation method is as follows: at first be through sterilization and disinfection, immersion, pulverizing, grinding and slurrying with kelp residue, obtain the marine alga slurry, with this marine alga slurry and biological bacteria liquid, oligochitosan, seawater and the sucrose thorough mixing, stirring, the fermentation that have prepared, finally become finished product then then.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102765776A (en) * | 2012-07-05 | 2012-11-07 | 昆明豪原特自控有限公司 | Active biological water purifying agent and preparation method thereof |
| CN103461237A (en) * | 2013-08-30 | 2013-12-25 | 中国科学院东北地理与农业生态研究所 | Estimation method for wetland adult-crab productive potential |
| CN104445639A (en) * | 2014-11-28 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Purifying agent for river crab aquaculture water |
| CN104710003A (en) * | 2015-02-12 | 2015-06-17 | 宁波市海洋与渔业研究院 | Purifying agent for improving shellfish cultivation environment |
| CN115011649A (en) * | 2022-07-07 | 2022-09-06 | 山东昌瑞生物科技有限公司 | Production method for preparing high-activity chitosan oligosaccharide by taking alaska crabs as raw materials |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1605571A (en) * | 2003-10-08 | 2005-04-13 | 上海创博生态工程有限公司 | Modifying agent for microbiological drinking water for cultivated animal and making method thereof |
| CN101691258A (en) * | 2009-10-15 | 2010-04-07 | 日照海韵环保生物科技发展有限公司 | Novel biological water purifying sphere and preparation process thereof |
| CN101691546A (en) * | 2009-10-15 | 2010-04-07 | 日照益康有机农业科技发展有限公司 | Liquid seaweed bio-fungus and preparation process thereof |
-
2010
- 2010-07-12 CN CN 201010227191 patent/CN101891307B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1605571A (en) * | 2003-10-08 | 2005-04-13 | 上海创博生态工程有限公司 | Modifying agent for microbiological drinking water for cultivated animal and making method thereof |
| CN101691258A (en) * | 2009-10-15 | 2010-04-07 | 日照海韵环保生物科技发展有限公司 | Novel biological water purifying sphere and preparation process thereof |
| CN101691546A (en) * | 2009-10-15 | 2010-04-07 | 日照益康有机农业科技发展有限公司 | Liquid seaweed bio-fungus and preparation process thereof |
Non-Patent Citations (1)
| Title |
|---|
| 《内陆水产》 20091231 张婷婷等 壳聚糖及其衍生物对水产养殖水质的净化作用 第47-49页 1-5 , 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102765776A (en) * | 2012-07-05 | 2012-11-07 | 昆明豪原特自控有限公司 | Active biological water purifying agent and preparation method thereof |
| CN103461237A (en) * | 2013-08-30 | 2013-12-25 | 中国科学院东北地理与农业生态研究所 | Estimation method for wetland adult-crab productive potential |
| CN104445639A (en) * | 2014-11-28 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Purifying agent for river crab aquaculture water |
| CN104710003A (en) * | 2015-02-12 | 2015-06-17 | 宁波市海洋与渔业研究院 | Purifying agent for improving shellfish cultivation environment |
| CN115011649A (en) * | 2022-07-07 | 2022-09-06 | 山东昌瑞生物科技有限公司 | Production method for preparing high-activity chitosan oligosaccharide by taking alaska crabs as raw materials |
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| CN101891307B (en) | 2013-01-16 |
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Effective date of registration: 20200902 Address after: Canzhuang Zhen Xiao Zhu Liu Cun, Zhaoyuan City, Yantai City, Shandong Province Patentee after: Shandong Pasteur Biotechnology Co., Ltd Address before: Ji'nan road 276800 in Shandong Province, Rizhao City Donggang District No. 77 Rhine sunshine Environmental Biological Technology Development Co. Ltd. Patentee before: RIZHAO YIKANG ORGANIC AGRICULTURAL TECHNOLOGY DEVELOPMENT Co.,Ltd. |
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