CN101082038A - Preparation of holothurian-benefit bacterium and method for restoring sea cucumber cultivation pool environment - Google Patents
Preparation of holothurian-benefit bacterium and method for restoring sea cucumber cultivation pool environment Download PDFInfo
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- CN101082038A CN101082038A CNA2006100271348A CN200610027134A CN101082038A CN 101082038 A CN101082038 A CN 101082038A CN A2006100271348 A CNA2006100271348 A CN A2006100271348A CN 200610027134 A CN200610027134 A CN 200610027134A CN 101082038 A CN101082038 A CN 101082038A
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- China
- Prior art keywords
- sea cucumber
- water
- seedling
- fry
- pond
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The sea cucumber benefiting bacterial preparation is composite live bacterial preparation for protecting sea cucumber fry and improving water quality in sea cucumber raising pond. It is developed based on the physiological and ecological features of sea cucumber fry and the characteristics of water and bottom material in sea cucumber raising pond and by means of microbial technology. It contains rejuvenated bacteriophagic Bdellovibrio as main probiotics, sea cucumber fry growth promoting factors, nourishing matters and immunological active matters. It is thrown regularly to sea cucumber fry pond to kill vibrio, fungi, cilibacillus and other harmful bacteria, to decompose organic pollutant in water, eliminate ammonia nitrogen, nitrite and other harmful matters, increase oxygen dissolved in water, purify water and bottom material and prevent diseases of sea cucumber fry.
Description
Benefit ginseng bacterium be special at the sea cucumber seed in the physiology of phase of growing seedlings, keep a full stand of seedings, ecological characteristic and ginseng pond water quality, substrate characteristics, the protection seedling health of using microbe engineering development, improve the compound active bacteria formulation of ginseng pond water quality.It is the main body probiotic bacterium with the bacteriophagic Bdellovibrio of selected rejuvenation, and is furnished with multiple seedling somatomedin and living nutritive substance of benefit and immunologic active material.Grow seedlings sea cucumber, during keeping a full stand of seedings, often throw in benefit ginseng bacterium to the seedling pond, can effectively engulf and kill the vibrios of Miao Chizhong, fungi, intestinal bacteria, Salmonellas, unwanted bacterias such as Aeromonas hydrophila, and decompose organic pollutant in the water body rapidly, eliminate ammonia nitrogen in the water, nitrite, objectionable impuritiess such as hydrogen sulfide, simultaneously can increase the water body dissolved oxygen, purify water and substrate, improve seedling anti-stress and resistance against diseases, seedling is infected the black matrix that occurs because of water quality deterioration and unwanted bacteria, shake the head, arrange dirty, swollen mouth, change skin, split intestines, diseases such as stiff body have the good preventing effect.Benefit ginseng bacterium is without any side effects to sea cucumber and other aquatic animals, the mankind and environment, and life-time service can not develop immunity to drugs, and is that the sea cucumber high-density is grown seedlings, kept a full stand of seedings and improves the seedling surviving rate, promotes growth, produces green seedling, the essential product of preserving the ecological environment.
[effect]
1, engulfs seedling pond unwanted bacteria, kill virulence factor;
2, improve seedling immunizing power, repair the damage of seedling somatocyte;
3, fast purification seedling pond water quality and substrate reduce and change water, reduce the cost of educating, keep a full stand of seedings;
4, all can grow well and breed in the suitable water temperature range of seedling, use throughout the year, protect seedling health throughout the year.
[physicochemical character] translucent liquid.
[main component] bacteriophagic Bdellovibrio, polypeptide, polysaccharide.
[active constituent content] every gram contains probiotic bacterium 2 * 10
7Individual.
[usage] evenly splashes with full pond, 10-20 times of Chi Shui dilution back.
[consumption]
1, grow seedlings: every cubic metre of water body 2-3 milliliter of splashing, splashed once every 2-3 days.
2, keep a full stand of seedings: every cubic metre of water body 3-5 milliliter of splashing, splashed once every 2-3 days.
3, seedling transportation: per 100 kilograms of fortune seedling water bodys add 100 milliliters of bacterium liquid.
4, seedling sterilization: add 200 milliliters of immersions of bacterium liquid seedling with 100 kilograms of Chi Shui and put into aquaculture water after 15-30 minute.
[attention]
1, throws in " benefit ginseng bacterium " first three day, ban use of the pharmaceutical chemicals and the antibiotic medicine of germicidal action in back five days;
2, use benefit ginseng bacterium not to be subjected to the influence of weather, water temperature, often use can guarantee that water quality is good, promotes the seedling growth;
3, benefit ginseng bacterium is safe in utilization, and multiplicable use when water quality deterioration is serious is used and can be changed water after 3 days.
[summary of the invention]
Probiotics of the present invention and preparation method thereof, as follows:
1. get peptone 5%, yeast leach liquor 5%, SODIUM PHOSPHATE, MONOBASIC 2%, Sodium phosphate dibasic 3%, sodium-chlor 5%, water 80%, it is standby to make nutrient solution.
2. get the bacteriophagic Bdellovibrio bacterial classification and 1. nutrient solution pour in the fermentor tank in 1: 49 ratio, keep pH6.5-8.5, temperature 25-32 ℃, fermentation culture 15-20 hour, after sampling detects, make bacteriophagic Bdellovibrio, standby.
3. yeast bacterium liquid is standby.
4. the xanthan gum of 80 mesh sieves choosing is standby.
5. get 2. and 3. and mix in 9: 1 ratio is composite.
6. get 4. by the 0.1-0.3% addition and add 5. in the mixed bacteria liquid, stir, place bottling after 2 hours.
The present invention has strong operability, with low cost, effect is remarkable, to the water surrounding non-secondary pollution, sea cucumber is had no side effect, the holothruian cultures bottom environment there are advantages such as obvious repairing effect, be to comply with current social back to nature, treasure water and life, pursue green food, the development healthy aquaculture, the only way of realizing healthy consumption.
Claims (1)
- Benefit ginseng bacterium be special at the sea cucumber seed in the physiology of phase of growing seedlings, keep a full stand of seedings, ecological characteristic and ginseng pond water quality, substrate characteristics, the protection seedling health of using microbe engineering development, improve the compound active bacteria formulation of ginseng pond water quality.It is the main body probiotic bacterium with the bacteriophagic Bdellovibrio of selected rejuvenation, and is furnished with multiple seedling somatomedin and living nutritive substance of benefit and immunologic active material.Grow seedlings sea cucumber, during keeping a full stand of seedings, often throw in this product to the seedling pond, can effectively engulf and kill the vibrios of Miao Chizhong, fungi, intestinal bacteria, Salmonellas, unwanted bacterias such as Aeromonas hydrophila, and decompose organic pollutant in the water body rapidly, eliminate ammonia nitrogen in the water, nitrite, objectionable impuritiess such as hydrogen sulfide, simultaneously can increase the water body dissolved oxygen, purify water and substrate, improve seedling anti-stress and resistance against diseases, seedling is infected the black matrix that occurs because of water quality deterioration and unwanted bacteria, shake the head, arrange dirty, swollen mouth, change skin, split intestines, diseases such as stiff body have the good preventing effect.[summary of the invention]Probiotics of the present invention and preparation method thereof, as follows:1. get peptone 5%, yeast leach liquor 5%, SODIUM PHOSPHATE, MONOBASIC 2%, Sodium phosphate dibasic 3%, sodium-chlor 5%, water 80%, it is standby to make nutrient solution.2. get the bacteriophagic Bdellovibrio bacterial classification and 1. nutrient solution pour in the fermentor tank in 1: 49 ratio, keep pH6.5-8.5, temperature 25-32 ℃, fermentation culture 15-20 hour, after sampling detects, make bacteriophagic Bdellovibrio, standby.3. yeast bacterium liquid is standby.4. the xanthan gum of 80 mesh sieves choosing is standby.5. get 2. and 3. and mix in 9: 1 ratio is composite.6. get 4. by the 0.1-0.3% addition and add 5. in the mixed bacteria liquid, stir, place bottling after 2 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100271348A CN101082038A (en) | 2006-05-31 | 2006-05-31 | Preparation of holothurian-benefit bacterium and method for restoring sea cucumber cultivation pool environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100271348A CN101082038A (en) | 2006-05-31 | 2006-05-31 | Preparation of holothurian-benefit bacterium and method for restoring sea cucumber cultivation pool environment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101082038A true CN101082038A (en) | 2007-12-05 |
Family
ID=38911823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2006100271348A Withdrawn CN101082038A (en) | 2006-05-31 | 2006-05-31 | Preparation of holothurian-benefit bacterium and method for restoring sea cucumber cultivation pool environment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101082038A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102017914A (en) * | 2010-08-31 | 2011-04-20 | 华南理工大学 | Energy-saving emission-reducing sea cucumber factory culture method |
| CN102017908A (en) * | 2010-08-31 | 2011-04-20 | 华南理工大学 | Water-saving sea cucumber culturing method |
| CN102037916A (en) * | 2010-08-31 | 2011-05-04 | 华南理工大学 | Application of bacterium solution of bdellovibrio bacteriovorus bdelloplasts in holothurian seedling raising |
| CN102057883A (en) * | 2010-08-31 | 2011-05-18 | 华南理工大学 | Application of bdellovibro swimmer bacterial liquid in culturing young sea cucumbers |
| CN102173529A (en) * | 2010-11-30 | 2011-09-07 | 福建凤竹纺织科技股份有限公司 | Deodorization method in treatment of printing and dyeing wastewater |
| CN102726634A (en) * | 2012-06-26 | 2012-10-17 | 格特生物制药（天津）有限公司 | Microecology feed for sea cucumbers, and preparation method thereof |
| CN108884336A (en) * | 2016-02-02 | 2018-11-23 | 法能集团 | Method for preventing biofouling on materials in contact with aqueous media |
| CN113564077A (en) * | 2021-07-28 | 2021-10-29 | 华侨大学 | A kind of Vibrio halophilic phage vibrio agent and preparation method and application thereof |
-
2006
- 2006-05-31 CN CNA2006100271348A patent/CN101082038A/en not_active Withdrawn
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102017914A (en) * | 2010-08-31 | 2011-04-20 | 华南理工大学 | Energy-saving emission-reducing sea cucumber factory culture method |
| CN102017908A (en) * | 2010-08-31 | 2011-04-20 | 华南理工大学 | Water-saving sea cucumber culturing method |
| CN102037916A (en) * | 2010-08-31 | 2011-05-04 | 华南理工大学 | Application of bacterium solution of bdellovibrio bacteriovorus bdelloplasts in holothurian seedling raising |
| CN102057883A (en) * | 2010-08-31 | 2011-05-18 | 华南理工大学 | Application of bdellovibro swimmer bacterial liquid in culturing young sea cucumbers |
| CN102017908B (en) * | 2010-08-31 | 2012-12-05 | 华南理工大学 | Water-saving sea cucumber culturing method |
| CN102017914B (en) * | 2010-08-31 | 2013-09-25 | 华南理工大学 | Energy-saving emission-reducing sea cucumber factory culture method |
| CN102173529A (en) * | 2010-11-30 | 2011-09-07 | 福建凤竹纺织科技股份有限公司 | Deodorization method in treatment of printing and dyeing wastewater |
| CN102726634A (en) * | 2012-06-26 | 2012-10-17 | 格特生物制药（天津）有限公司 | Microecology feed for sea cucumbers, and preparation method thereof |
| CN108884336A (en) * | 2016-02-02 | 2018-11-23 | 法能集团 | Method for preventing biofouling on materials in contact with aqueous media |
| CN113564077A (en) * | 2021-07-28 | 2021-10-29 | 华侨大学 | A kind of Vibrio halophilic phage vibrio agent and preparation method and application thereof |
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