CN106854629A - A kind of high concentration composite bacillus and its preparation and application - Google Patents

A kind of high concentration composite bacillus and its preparation and application Download PDF

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Publication number
CN106854629A
CN106854629A CN201510901402.3A CN201510901402A CN106854629A CN 106854629 A CN106854629 A CN 106854629A CN 201510901402 A CN201510901402 A CN 201510901402A CN 106854629 A CN106854629 A CN 106854629A
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China
Prior art keywords
bacillus
concentration composite
liquid
composite bacillus
concentration
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Pending
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CN201510901402.3A
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Chinese (zh)
Inventor
邹国忠
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Wuxi Hongbao Microorganism Technology Co., Ltd
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Shanghai Hongbao Green Aquatic Product Co Ltd
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Priority to CN201510901402.3A priority Critical patent/CN106854629A/en
Publication of CN106854629A publication Critical patent/CN106854629A/en
Pending legal-status Critical Current

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Abstract

The invention provides a kind of high concentration composite bacillus, it is characterised in that be prepared from by the component of following mass percent:Polysaccharide macromolecular compound 0.5-5;Micro- 0-5;Bacterial Physiological salt solution 40-60;Liquid bacillus 25-60.The high concentration composite bacillus that the present invention is provided are applied to all seawater and fresh-water fishes, shrimp, crab, shellfish, ginseng nursery pond and growing pond, are particularly suited for the aging pond that too high cultivation density, water quality overfertilization, disease take place frequently.

Description

A kind of high concentration composite bacillus and its preparation and application
Technical field
The present invention relates to aquatic biological cultivation field, in particular it relates to a kind of high concentration composite bacillus And its preparation and application.
Background technology
Breeding water body is the ecological environment of artificial manufacture, is a water body for synthesis, is various biological flourishing The relation of symbiosis.In this environment, all biological species are closely bound up, adjust any ring, Or the variation of any ring, can all have influence on whole water body.
It is the important embodiment that it produces various digestive ferments that bacillus can improve breeding performonce fo animals.Grind Study carefully and show, bacillus can produce various digestive ferments, help animal to digest and assimilate nutriment.Bud Spore bacillus has stronger protease, amylase and lipase active, while multiple in also having degraded feed The enzyme of miscellaneous carbohydrate, such as pectase, dextranase, cellulase, these enzymes can destroy plant The cell membrane of thing feed cell, promotes the nutriment of cell to discharge, and can eliminate anti-in feed Trophic factors, reduces the obstacle that ANFs is utilized to animal digestion.
But, for a long time liquid bacillus be difficult to preserve turn into a big technological difficulties, therefore, its There is significant limitation in aquaculture.
The content of the invention
It is contemplated that overcoming drawbacks described above, there is provided one kind be applied to all seawater and fresh-water fishes, shrimp, crab, Shellfish, ginseng nursery pond and growing pond, are particularly suited for too high cultivation density, water quality overfertilization, disease and take place frequently The high concentration composite bacillus in aging pond.
Specifically, a kind of high concentration composite bacillus that the present invention is provided, it is characterised in that by as follows The component of mass percent is prepared from:
Further, high concentration composite bacillus of the present invention, also with following features:I.e., Above-mentioned polysaccharide macromolecular compound is selected from starch, cellulose, glycogen, glucose, mannosan, half One or more in newborn glycan, acid heteroglycan.When from multiple compound of polysaccharide, all kinds of chemical combination The consumption of thing is 0.1-3%.
Further, high concentration composite bacillus of the present invention, also with following features:I.e., Above-mentioned cellulose is selected from the cellulosic cpd of sodium carboxymethylcellulose and its similar structures.
Further, high concentration composite bacillus of the present invention, also with following features:I.e., Above-mentioned acid heteroglycan is selected from the acid heteroglycan compound of xanthans and its similar physicochemical property.
Further, high concentration composite bacillus of the present invention, also with following features:I.e., Aforesaid liquid bacillus is selected from liquid bacillus subtilis, liquid bacillus cereus, liquid natto bud In spore bacillus, liquid bacillus thuringiensis, liquid bacillus licheniformis, liquid Paenibacillus polymyxa One or more.When from various bacterium, the consumption of all kinds of bacterium is 10-25%.
Further, high concentration composite bacillus of the present invention, also with following features:I.e., It is prepared from by the component of following mass percent:
Further, high concentration composite bacillus of the present invention, also with following features:I.e., Probiotics at least 2 × 10 is included in above-mentioned every gram of high concentration composite bacillus8It is individual.
Additionally, present invention also offers the preparation method of above-mentioned high concentration composite bacillus, its feature exists In:Step one, by Bacterial Physiological salt solution, liquid bacillus, micro- mixing and stirring;
Step 2, the addition polysaccharide macromolecular compound in above-mentioned mixed solution, are into after stirring Product.
Additionally, present invention also offers the application method of above-mentioned high concentration composite bacillus, its feature exists In:Finished product prepared by the above method is uniformly splashed in full pond after being diluted with 40-50 times of water.
Effect of the invention and effect:
The high concentration composite bacillus that the present invention is provided be applied to all seawater and fresh-water fishes, shrimp, crab, Shellfish, ginseng nursery pond and growing pond, are particularly suited for too high cultivation density, water quality overfertilization, disease and take place frequently Aging pond.
The present invention selects high-effect special Bacillus, microbe ultrarapture liquid fermentation technique to open The high concentration composite bacillus of hair, it overcomes liquid bacillus for a long time and is difficult to the technology for preserving Difficult point.
After delivering water body, dormancy viable bacteria is in water body, can recover quickly in the presence of the heavy-oxygen-enriched water of upper strata and Disintegration, and dominant microflora is multiplied into multiple, and spread go deep into water body, lower floor, when active Bacillus After occupying superiority in water body, can be competed by nutrient competition, place and secretion resisting substance, directly Connect or suppress harmful bacteria, the breeding of algae in water body indirectly, the organic pollution in fast decoupled water body, For beneficial algae provides nutriment, promote alga cells vigor, so as to reach rich water running water double effects.
Specific embodiment
Embodiment one, high concentration composite bacillus 1# are prepared from by the component of following mass percent:
The preparation method of above-mentioned high concentration composite bacillus 1# is:By Bacterial Physiological salt solution, liquid natto After Bacillus, liquid bacillus subtilis, micro- mixing and stirring;Add carboxymethylcellulose calcium Receive, glycogen, after stirring be finished product.
Embodiment two, high concentration composite bacillus 2# are prepared from by the component of following mass percent:
The preparation method of above-mentioned high concentration composite bacillus 2# is:By Bacterial Physiological salt solution, liquid natto After Bacillus, micro- mixing and stirring;Mannosan, starch are added, is into after stirring Product.
Embodiment three, high concentration composite bacillus 3# are prepared from by the component of following mass percent:
The preparation method of above-mentioned high concentration composite bacillus 3# is:By Bacterial Physiological salt solution, liquid natto After Bacillus, liquid bacillus subtilis, liquid bacillus licheniformis mixing and stirring;Add carboxylic first Ji Xianweisuna, is finished product after stirring.
Example IV, high concentration composite bacillus 4# are prepared from by the component of following mass percent:
The preparation method of above-mentioned high concentration composite bacillus 4# is:By Bacterial Physiological salt solution, liquid natto After Bacillus, liquid bacillus subtilis, micro- mixing and stirring;Add glucose, xanthan Glue, sodium carboxymethylcellulose, are finished product after stirring.
Embodiment five, high concentration composite bacillus 5# are prepared from by the component of following mass percent:
The preparation method of above-mentioned high concentration composite bacillus 5# is:By Bacterial Physiological salt solution, liquid natto After Bacillus, liquid bacillus subtilis mixing and stirring;CMC, xanthans are added, It is finished product after stirring.
Embodiment six, high concentration composite bacillus 6# are prepared from by the component of following mass percent:
The preparation method of above-mentioned high concentration composite bacillus 6# is:By Bacterial Physiological salt solution, liquid natto After Bacillus, liquid bacillus subtilis, liquid bacillus thuringiensis mixing and stirring;Add sweet Dew glycan, is finished product after stirring.
Embodiment seven, high concentration composite bacillus 7# are prepared from by the component of following mass percent:
CMC 4;
Bacterial Physiological salt solution 36;
Liquid bacillus subtilis 60.
The preparation method of above-mentioned high concentration composite bacillus 7# is:By Bacterial Physiological salt solution, liquid withered grass After bacillus mixing and stirring;CMC is added, is finished product after stirring.
The application method of above-mentioned 1-7# products:
This product is diluted 40-50 times with water, full pond is uniformly splashed after stirring evenly.
1st, water quality is improved:Every mu of water surface (1 meter of the depth of water) uses 250-500 milliliters of this product, is sprinkled every 7-10 days Spill once, first using or multiplicable during water quality deterioration splash.
2nd, nursery:Every cube water splashing 3-5 milliliters.
Points for attention:
1st, " Bacillus " first three day is delivered, the chemicals of bactericidal action is prohibitted the use of in five days afterwards and is resisted Raw element medicine;
2nd, during use, dissolved oxygen in water should be maintained at more than 3 mg/litres;
3rd, using it is preceding first bacterium solution in bottle is shaken up after uncap use again, please be timely use after uncapping;
4th, weather, water temperature and illumination effect are not received using this product, commonly using can ensure that water quality is good;
5th, using effect is more preferably for this product cooperation " deep precious EM bacterium ".
Embodiment is related to the using effect of product:
This product is applied to the cultivating pools such as all seas, fresh-water fishes, shrimp, crab, shellfish, sea cucumber, especially fits Close the more aging pond of mud.
Residual feed and excreta in this product energy efficient-decomposition water, eliminates sludge underwater, purifies water;Promote Swim the growth of unicellular alga, balance algae phase, suppress the breeding of harmful algae;Effective elimination ammonia nitrogen, The harmful substances such as nitrite, hydrogen sulfide;Alleviate the aquatic livestock caused by pond toxin is accumulated not take food, Vigor is weak, and body colour is light, the reaction of the distress such as growth is slow;Moreover it is possible to reduce making for antibiotic medicine With improvement aquatic products body colour and quality.
In 25 DEG C -28 DEG C of culturing pool, the product effectively suppresses harmful algae breeding after 3 days is delivered, Sampling inspection results display inhibiting rate relatively contrasts pond (not adding this product) and improves more than 15%, ammonia nitrogen, Asia The content of the harmful substances such as nitrate is the 50-65% for contrasting pond content, (is reduced to later using one week 10-15%, is 0-5% using after two weeks), the speed of growth reduction by more than 28% of harmful algae in water body, One culture-cycle aquatic livestock speed of growth increases 15-20%.

Claims (9)

1. a kind of high concentration composite bacillus, it is characterised in that be prepared from by the component of following mass percent:
2. a kind of high concentration composite bacillus as claimed in claim 1, it is characterised in that:
The polysaccharide macromolecular compound is selected from starch, cellulose, glycogen, glucose, mannosan, gala One or more in glycan, acid heteroglycan.
3. a kind of high concentration composite bacillus as claimed in claim 1, it is characterised in that:
The cellulose is selected from sodium carboxymethylcellulose.
4. a kind of high concentration composite bacillus as claimed in claim 1, it is characterised in that:
The acid heteroglycan is selected from xanthans.
5. a kind of high concentration composite bacillus as claimed in claim 1, it is characterised in that:
The liquid bacillus is selected from liquid bacillus subtilis, liquid bacillus cereus, liquid natto gemma In bacillus, liquid bacillus thuringiensis, liquid bacillus licheniformis, liquid Paenibacillus polymyxa one Plant or several.
6. a kind of high concentration composite bacillus as claimed in claim 1, it is characterised in that by following quality hundred The component of ratio is divided to be prepared from:
7. a kind of high concentration composite bacillus as described in claim 1-6 is any, it is characterised in that:
Probiotics at least 2 × 10 is included in every gram of high concentration composite bacillus8It is individual.
8. a kind of preparation method of the high concentration composite bacillus as described in claim 1-6 is any, its feature exists In:
Step one, by Bacterial Physiological salt solution, liquid bacillus, micro- mixing and stirring;
Step 2, the addition polysaccharide macromolecular compound in above-mentioned mixed solution, are finished product after stirring.
9. a kind of application method of the high concentration composite bacillus as described in claim 1-6 is any, its feature exists In:
Finished product prepared by method as claimed in claim 8 is uniformly splashed in full pond after being diluted with 40-50 times of water.
CN201510901402.3A 2015-12-08 2015-12-08 A kind of high concentration composite bacillus and its preparation and application Pending CN106854629A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108048340A (en) * 2017-10-10 2018-05-18 盐城裕达饲料有限公司 A kind of bacillus cereus and its application
CN108815200A (en) * 2018-07-09 2018-11-16 江西省科学院生物资源研究所 A kind of bacillus combination preparation and its preparation method and application
CN108815202A (en) * 2018-07-09 2018-11-16 江西省科学院生物资源研究所 A kind of chitosan microbial compound preparation and its preparation method and application
CN110741981A (en) * 2019-12-04 2020-02-04 范秀娟 Water fertilizing method for aquaculture water body

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011142300A1 (en) * 2010-05-12 2011-11-17 天野エンザイム株式会社 Reducing agent from microorganism belonging to genus bacillus and application for same
CN102517237A (en) * 2011-12-30 2012-06-27 湖南农业大学 Composite micro-ecological water-based suspension concentrate for aquaculture
CN102925377A (en) * 2011-11-28 2013-02-13 上海海洋大学 Bacillus licheniformi, screening method and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011142300A1 (en) * 2010-05-12 2011-11-17 天野エンザイム株式会社 Reducing agent from microorganism belonging to genus bacillus and application for same
CN102892882A (en) * 2010-05-12 2013-01-23 天野酶株式会社 Reducing agent from microorganism belonging to genus bacillus and application for same
CN102925377A (en) * 2011-11-28 2013-02-13 上海海洋大学 Bacillus licheniformi, screening method and uses thereof
CN102517237A (en) * 2011-12-30 2012-06-27 湖南农业大学 Composite micro-ecological water-based suspension concentrate for aquaculture

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108048340A (en) * 2017-10-10 2018-05-18 盐城裕达饲料有限公司 A kind of bacillus cereus and its application
CN108048340B (en) * 2017-10-10 2021-05-04 盐城裕达饲料有限公司 Bacillus cereus and application thereof
CN108815200A (en) * 2018-07-09 2018-11-16 江西省科学院生物资源研究所 A kind of bacillus combination preparation and its preparation method and application
CN108815202A (en) * 2018-07-09 2018-11-16 江西省科学院生物资源研究所 A kind of chitosan microbial compound preparation and its preparation method and application
CN108815202B (en) * 2018-07-09 2019-07-30 江西省科学院生物资源研究所 A kind of chitosan microbial compound preparation and its preparation method and application
CN108815200B (en) * 2018-07-09 2020-08-28 江西省科学院生物资源研究所 Bacillus composite preparation and preparation method and application thereof
CN110741981A (en) * 2019-12-04 2020-02-04 范秀娟 Water fertilizing method for aquaculture water body

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Effective date of registration: 20191118

Address after: E Hu Zhen San Xin Cun Hong Bao Yuan, Xishan District, Wuxi City, Jiangsu Province

Applicant after: Wuxi Hongbao Microorganism Technology Co., Ltd

Address before: 200233 room 33, building 680, No. 303-1, Guiping Road, Shanghai, Xuhui District

Applicant before: SHANGHAI HONGBAO GREEN AQUATIC PRODUCT CO., LTD.

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Application publication date: 20170616

RJ01 Rejection of invention patent application after publication