CN1579200A - Multi-effect micro-biological feed additive and its preparation method - Google Patents
Multi-effect micro-biological feed additive and its preparation method Download PDFInfo
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- CN1579200A CN1579200A CNA031400264A CN03140026A CN1579200A CN 1579200 A CN1579200 A CN 1579200A CN A031400264 A CNA031400264 A CN A031400264A CN 03140026 A CN03140026 A CN 03140026A CN 1579200 A CN1579200 A CN 1579200A
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Abstract
The invention relates to a microbial feed additive and its preparing method, and its characteristic: it uses lactic acid bacilli that have the characters of lactobacilli and bacilli and uses special fermentation culture to prepare a fermentation liquor that has large numbers of lactic acid bacilli and high spore forming rate to act as the feed additive. It has an obvious function of nursing stomach intestine of the fed animal, and can increase the assimilation of the fed animal to the feed and enhancing the disease resisting ability of the fed animal, especially applied to act as the feed additive of domestic animal and fowl and aquatic animal.
Description
Technical field
The present invention relates to a kind of microorganism feed addictive and preparation method thereof, especially be suitable for the fodder additives of doing fowl poultry, aquatic animal.
Background technology
Microbiotic uses the history in existing 50 years as fodder additives, has made huge contribution for growth and the health of animal, is one of consumption maximum in the present feed, the widest additive.Since Japan in 1975 reports pathogenic bacteria resistance phenomenon the earliest, it is found that, owing to a large amount of and abuse of antibiotics bring a series of problem to animal itself, animal product and environment.Show that mainly animal pathogen develops immunity to drugs, cause the increase of antibiotic dosage, cause autogenous infection and superinfection.Particularly life-time service can cause the pollution of interior little ecologic disturbance of digestive tube own and environment.The residual also directly influence mankind's the immunity and healthy of microbiotic in product simultaneously.The feed microbe additive is because of having safety, environmental protection, having no side effect and characteristics such as disease-resistant, promotes growth, and be subjected to numerous scholars' attention, and carried out a large amount of research from many-sides such as the screening of microecology theory, the mechanism of action, bacterial classification and application, obtained gratifying achievement.As the substitute of antibiotics additive, the feed microbe additive at home and abroad all is widely used, has obtained very ideal effect, is one of the most promising fodder additives.The feed microbe additive belongs to the category of probiotics or probiotics.It develops with animal microecology (comprising the microecological balance theory, little ecologic disturbance theory, the theoretical and little bionomic control theory of little ecoalimental) is theoretical foundation.The feed microbe additive is meant takes in the living microorganism culture that participates in microbial balance in the intestines in the animal body, it can be by directly strengthening the restraining effect of animal to intestinal toxic microflora, perhaps come preventing disease by strengthening non-specific immune function, and the effect of playing the promotion growth of animal indirectly and improving food conversion ratio.Numerous experiment confirms have and improve weightening finish, improve feed efficiency, promote the livestock and poultry body's immunity, reduce mortality ratio and improve effect such as environment.As improve fryer surviving rate, weightening finish and feed efficiency; Improve the laying hen living environment, improve the quality of egg laying performance and egg; Improve the piglet survival ratio and the speed of growth, effectively prevent grice diarrhoea; Improve fertility big porker day weight gain, add that in the sow daily ration number pigs weaned and weanling weight are significantly improved.In aquaculture, microorganism feed addictive can improve the surviving rate of Luo Shi prawn seedling, is enhanced to shrimp, the Atlantic salmon resistibility to pathogenic bacteria; And the photosynthetic bacterium in the additive and chemoheterotrophic bacteria can degrade and enter various organic waste in the cultivating pool, eliminates the poisonous factor, stablizes the pH value, and balance bacterium and algae are built good aquatic environment mutually, reach the effect of preventing disease, healthy aquaculture.At present the probiotics that uses mainly is the preparation of single culture such as genus bacillus, lactobacillus, streptococcus faecium or composite bacteria.
The bacterial classification that microorganism feed addictive adopts mainly contains lactic acid bacteria class (Lacticacid bacteria), budding fungus (Yeasts) and bacillus category (Bacillus).
The genus bacillus that is used for fodder additives is a kind of aerobic non-pathogenic bacteria.It all exists with interior form of giving birth to son in enteron aisle or storage, does not consume feed nutrient, can keep quality of the fodder.After entering enteron aisle, bring back to life rapidly in upper intestines, the resurrection rate is near 100%.It can make the pH value in the jejunum descend, and ammonia concentration reduces, and consumes a large amount of oxygen, keeps the enteron aisle anaerobic environment, suppresses pathogen growth, keeps the normal eubiosis of enteron aisle, and the effect of balance and stabilizing lactic acid bacteria is arranged.It can produce active very high proteolytic enzyme, amylase, lipase and multiple amino acids.The genus bacillus strong stress resistance has characteristics such as high temperature resistant, acid and alkali-resistance, anti-extruding, can tolerate the influence of granulated feed processing.
Lactic acid bacteria class is a kind ofly can decompose the no gemma gram-positive microorganism that carbohydrate produces lactic acid, can anaerobism, the amphimicrobian growth.Milk-acid bacteria has consequence in fodder additives, having nearly 30 kinds in more than the 40 kind of microorganism fodder that U.S. FAD (1989) announces is milk-acid bacteria.It is acidproof, is still can grow in 3.0～4.5 o'clock at pH, is adapted to the sour environment of stomach and intestine.Milk-acid bacteria is the normal microflora of fish enteron aisle, and in the field planting of fish body intestinal tract, in vivo by biological antagonist, the antagonism gram negative pathogenic bacteria is kept the normal eubiosis in the enteron aisle, can reduce enteron aisle pH value, stops and suppress objectionable impurities, strengthens anti-infection ability.Contain higher superoxide-dismutase (SOD) in the viable bacteria body and in the meta-bolites, can strengthen humoral immunization and the cellular immunization of animal, the immunoregulatory activity of enhancing body promotes growth.But such bacterium does not form gemma in process of growth, resistance is poor, and easily inactivation be cannot say for sure to hide, so the application in fodder industry is subjected to certain restriction.The microcapsule coating technique is adopted in some commercially available prod at present, has improved its resistance to environment, but the also corresponding raising of production cost.
Yeast is the aerophil that happiness is grown in the slant acidity environment, breeding in a large number in enteron aisle.In water industry, can use the yeast that is separated to from fish body, water body, mainly contain mycocandida (Candida) etc.Also can use brewer's yeast (Brewer ' s yeast) and bread yeast (Baker ' s yeast) in the feed.
Summary of the invention
The objective of the invention is to develop more superior microorganism feed addictive of a kind of performance and preparation method thereof.
The present invention utilizes lactic acid bacillus except having all beneficial characteristics of ordinary lactic acid bacteria common, also have the characteristic of genus bacillus concurrently, prepare by special fermentation culture and to contain the lactic acid bacillus bacterium and count the high fermented liquid of height, gemma rate of formation, realized purpose of the present invention thereby performance is better than existing microorganism feed addictive as fodder additives.
The technical characterictic of multiple-effect microbial fodder additives of the present invention is to contain the fermented liquid that contains lactic acid bacillus that is obtained by following preparation method, and described preparation method comprises following several steps:
(a) slant strains activation: preservation inclined-plane kind lactic acid bacillus lined respectively on the inclined-plane kind substratum activate, contain peptone 10～20.0g/L in the described inclined-plane kind substratum, yeast extract paste or extractum carnis or corn steep liquor 3.0～10.0g/L, glucose 5～15.0g/L, agar 18～22g/L, all the other are water, pH7.0～7.2, ordinary method sterilization;
(b) level liquid of bacterial classification activates: above-mentioned cultured inclined-plane kind is inoculated in respectively in the level liquid substratum with transfering loop cultivates, make the bacterium number of liquid strain reach 1.0 * 10
9～5.0 * 10
9More than the CFU/mL, contain peptone 15.0～25g/L in the described level liquid substratum, yeast extract paste or extractum carnis or corn steep liquor 5.0～15.0g/L, glucose 10.0～30.0g/L, calcium salt 5.0～15g/L, sal epsom 0.1～1.0g/L, manganese salt 0.01～0.1g/L, pH7.0～7.5, all the other are water, the ordinary method sterilization;
(c) cultivation in the seeding tank: above-mentioned cultured respectively level liquid kind is shaken up in the substratum of back seeding tank of mark 1%～5% inoculum size access by volume under aseptic condition, aerated culture 24～48h treats that the bacterium number reaches 1.0 * 10
9～5.0 * 10
9More than the CFU/mL, stop to cultivate;
(d) cultivation in the big fermentor tank: with above-mentioned cultured respectively lactic acid bacillus kind by volume the inoculum size of mark 10%～20% insert in the substratum of fermentor tank and cultivate, 37～42 ℃ of temperature, rotating speed 180～250r/min is by every m
3The air of substratum is 20～30m
3/ h carries out aerated culture 48～60h, begins to add sterilized glucose concentrated solution behind fermentation 16～40h, and total additional amount is a volume fraction 1%～4%, treats that the bacterium number reaches 1.0 * 10
9～5.0 * 10
9More than the CFU/mL, stop fermentation culture;
(c) and (d) substratum described in the step contains peptone 20.0～30.0g/L, yeast extract paste or extractum carnis or corn steep liquor 5.0～15g/L, glucose 10.0～40.0g/L, calcium salt 5.0～15.0g/L, sal epsom 0.1～0.5g/L, manganese salt 0.01～0.1g/L, all the other are water, pH7.0～7.5, the ordinary method sterilization.
(b), (c) and (d) calcium salt described in the step can be lime carbonate, calcium sulfate, nitrocalcite etc., lime carbonate preferably, described manganese salt can be manganous sulfate, Manganous chloride tetrahydrate, manganous nitrate etc., preferably manganous sulfate.
Preferably also contain massfraction 2% ore powder, massfraction 2% zeolite powder, massfraction 2% wilkinite, massfraction 5% citric acid in the multiple-effect microbial fodder additives of the present invention.
The used lactic acid bacillus of the present invention also is Bacillus coagulans, and bacterium number is Bacillus coagulansAB92064, is preserved in CCTCC, Chinese typical culture collection center.This bacterial classification " Chinese typical culture collection center culture catalogue " (Tao Tianshen. Wuhan University, 1998.25.) go up open record.
The preparation method of multiple-effect microbial fodder additives of the present invention comprises above-mentioned (a) and (b), (c) and (d) step.
The preparation method of multiple-effect microbial fodder additives of the present invention is preferably in the sophisticated fermented liquid that (d) step produces and adds massfraction 2% ore powder, massfraction 2% zeolite powder, massfraction 2% wilkinite, massfraction 5% citric acid, stir, mix, promptly make the multiple-effect microbial fodder additives.
This multiple-effect microbial fodder additives can mix with feed or bait in the ratio of massfraction 1%～3%, is used for nutrition purposes, especially for feeding animals, increases feeding animals to the absorption of feed and the resistance against diseases of raising feeding animals.
The present invention has following advantage.
1, the used lactic acid bacillus of the present invention possesses following characteristic as the bacterial classification of ideal microbe additive: (1) has good security, and used bacterial classification can not make humans and animals cause a disease, and does not produce cross-fertilize seed under field conditions (factors) with pathogenic micro-organism; (2) be easy to cultivate, reproduction speed is fast; (3) good field planting ability is arranged, in low pH value and bile, can survive, and can implant intestinal mucosa; (4) can produce materials such as acid and hydrogen peroxide during the fermentation; (5) can synthesize the inhibitions of aquatic animal pathogen enterobacterias such as intestinal bacteria, vibrios, Aeromonas and do not influence oneself activity, help promoting growing and disease resistance enhancemen of host; (6) can keep vitality in whole preparation and preservation process, it is good to sneak into the feed rear stability;
2, the wilkinite of the present invention's adding, zeolite powder etc. contain a large amount of trace quantity mineral substance elements, and it can act on the feeding animals performance is following: (1) is to the direct effect of immune organ and immunocyte; (2) effect of cofactor; (3) bacteriostatic action; (4) trace quantity mineral substance element can alleviate stress reaction as effect, the anti-oxidant and anti-aging effect of trace quantity mineral substance element, trace quantity mineral substance element participation complementary reaction, the trace quantity mineral substance element of immunostimulant.
3, because the animal stomach is a sour environment, bacterium wherein mostly is acid-producing bacteria and aciduric bacteria, growing animal secretion hydrochloric acid in gastric juice ability a little less than, and make foreign bacteria survival and breeding easily.The citric acid that the present invention adds can improve gastric juice acidity as souring agent, promote the big breedings of aciduric bacteria such as lactic acid bacillus, milk-acid bacteria, the status of bacterium makes it to keep on top under one's belt, foreign bacterias such as intestinal bacteria then can not adapt to sour environment, and be subjected to milk-acid bacteria etc. " squeezing " and can not the field planting survival, therefore can reduce the incidence of the rational diarrhoea of zoonosis.Souring agent can also be adjusted immune system response by the help machine, strengthens the disease resistance of poultry.
4, preparation method of the present invention makes the lactic acid bacillus bacterium number in the fermented liquid reach 1.0 * 10
9More than the CFU/mL, the gemma rate of formation is more than 70%.Because gemma rate of formation height, make lactic acid bacillus AB92064 to pass stomach smoothly and be colonizated in enteron aisle, lactic acid bacillus is except that having all beneficial characteristics of ordinary lactic acid bacteria common, also have the characteristic of genus bacillus concurrently, at resist drying, high temperature resistance, antiacid degree aspect is better than ordinary lactic acid bacteria, therefore can a large amount of field planting in enteron aisle.This bacterium has tangible conditioning functions to the feeding animals gi tract, can increase feeding animals to the absorption of feed and the resistance against diseases of raising feeding animals.
Embodiment
Embodiment 1:
Use 2m
3Fermentor tank is produced the multiple-effect microbial fodder additives and is comprised with several steps:
(a) slant strains activation: line on the inclined-plane kind substratum of new preparation with bacterial classification lactic acid bacillus AB92064 inoculation one ring of inoculating needle with preservation, the chemical constitution of inclined-plane kind substratum following (g/L): peptone 15.0, yeast extract paste 5.0, glucose 10.0, agar 20, all the other are water, pH7.0; Each is planted respectively to connect and props up, and cultivates 48 hours in 40 ℃;
(b) level liquid of bacterial classification is cultivated: cultured inclined-plane kind is inoculated one respectively with transfering loop encircle in the level liquid activation medium, level liquid substratum chemical constitution following (g/L): peptone 20.0, yeast extract paste 10.0, glucose 20.0, CaCO
310.0, MgSO
47H
2O 0.2, MnSO
44H
2O 0.05, pH7.2; Substratum is loaded in the 1000mL triangular flask, and the triangular flask loading amount is 40mL, and each kind respectively connects 4 bottles, and in 40 ℃ of reciprocating type shaking table shaking culture 36～48 hours, shaking speed was 120r/min, and stroke is 75mm, makes the bacterium number of liquid strain reach 1.0 * 10
9More than the CFU/mL;
(c) cultivation in the 250L seeding tank: above-mentioned cultured respectively level liquid kind is shaken up during the back is blended under aseptic condition in the connecing of an aseptic 1L bottle, inserting seeding tank then cultivates, the chemical constitution of seed tank culture base following (g/L): peptone 25.0, yeast extract paste 10.0, glucose 40.0, CaCO
310.0, MgSO
47H
2O 0.2, MnSO
4H
2O 0.05, and all the other are water, pH7.2; The canned 160L substratum of 250L seed, 38 ℃, rotating speed 200r/min, air quantity are 2m
3/ h carries out aerated culture, keeps tank pressure 0.1MPa, treats that the bacterium number reaches 1.0 * 10
9More than the CFU/mL, stop to cultivate;
(d) 2m
3Cultivation in the big fermentor tank: above-mentioned cultured respectively kind of 160L inserted to be equipped with in the big fermentor tank that disinfects substratum cultivate fermention medium component (g/L): peptone 25.0, yeast extract paste 10.0, glucose 40.0, CaCO
310.0, MgSO
47H
2O 0.2, MnSO
44H
2O 0.05, and all the other are water, pH7.2; 2m
3The canned 1300L substratum of big fermentation, 38～40 ℃, rotating speed 220r/min, air quantity are air 140m
3/ h fermented 24 hours, added the aseptic Glucose Liquid 28L of 500g/L, fermented 48 hours, added the aseptic Glucose Liquid 28L of 500g/L again, treated that bacteria concentration reaches 1.0 * 10
9More than the CFU/mL, stop fermentation culture;
(e) make finished product: after stopping fermentation culture, at first close vent-pipe, turn off agitator motor, open manhole, add 28 kilograms of ore powders, 28 kilograms of zeolite powders, 28 kilograms of wilkinites, citric acid 70L; Cover manhole again, open agitator motor, fully stirred 10 minutes, reduce mixing speed, begin to put jar, put into the jar of packing of different volumes directly, fast, promptly make finished product with 2 flexible pipes to 80r/min with 160r/min.
Embodiment 2: the product application effect test
The multiple-effect microbial fodder additives that embodiment 1 makes, carry out carp and grass carp are carried out application test, the result is as follows: add massfraction 0.1% fish microbe additive group of the present invention in 15～17g carp fry bait, equal rate of body weight gain of its tail and surviving rate are respectively than control group many 8.5% and 14.1%; Cabrage 49～53g carp adds massfraction 0.1% fish microbe additive group of the present invention in the feed, the equal rate of body weight gain of its tail is than control group many 8.64%; The grass carp of the carp of cabrage 51～61g and cabrage 98～116g is raised together with, it is many respectively 14.5% and 8.5% than control group to add massfraction 0.1% fish microbe additive group equal rate of body weight gain of carp tail of the present invention and surviving rate in the bait, and equal rate of body weight gain of grass carp tail and surviving rate are than control group respectively many 30.2% and 44.6%; To high, medium and low three horizontal comparison tests of food nutrition, best with the equal rate of body weight gain of tail of the middle trophic level group of digestible energy 3800 kilocalorie/kilograms and crude protein 32%, the higher trophic level group is many 31%, Duos 0.13% than the low nutrient level group; Additive as pig feed is fed the seedling pig, prevents and treats yellow and white dysentery of piglet effectively, reduces diarrhea frequency more than 65%, improves piglet beverage transformation efficiency 16%, and day weight gain improves 21%.When using in the full phase of store pig, average daily gain can reach more than the 780g at least, improves more than 15% than control group; Feed laying hen and fryer as the additive of chicken feed, can make laying rate of laying hen improve about 15%～20%, it is about 8% that fryer is increased weight the same period, improves the surviving rate of seedling chicken, shortens breeding cycle, delivers for sale in advance, reduces feedstuff-meat ratio; And, can make the prawn seedling rate improve about 30% as the fodder additives of fishes and shrimps.The result shows that lactic acid bacillus has security as microbe additive, and can promote growth, improve bait utilization and culture surviving rate, and in or the effect of adding the multiple-effect microbial fodder additives in the feed of low plane of nutrition show more obviously, effect is better than high-plane of nutrition, so the present invention is a kind of ideal fodder additives.
Claims (6)
1. a multiple-effect microbial fodder additives is characterized in that containing the fermented liquid that contains lactic acid bacillus that is obtained by following preparation method, and described preparation method comprises following several steps:
(a) slant strains activation: preservation inclined-plane kind lactic acid bacillus lined respectively on the inclined-plane kind substratum activate, contain peptone 10～20.0g/L in the described inclined-plane kind substratum, yeast extract paste or extractum carnis or corn steep liquor 3.0～10.0g/L, glucose 5～15.0g/L, agar 18～22g/L, all the other are water, and pH 7.0～7.2, the ordinary method sterilization;
(b) level liquid of bacterial classification activates: above-mentioned cultured inclined-plane kind is inoculated in respectively in the level liquid substratum with transfering loop cultivates, make the bacterium number of liquid strain reach 1.0 * 10
9～5.0 * 10
9More than the CFU/mL, contain peptone 15.0～25g/L in the described level liquid substratum, yeast extract paste or extractum carnis or corn steep liquor 5.0～15.0g/L, glucose 10.0～30.0g/L, calcium salt 5.0～15g/L, sal epsom 0.1～1.0g/L, manganese salt 0.01～0.1g/L, pH 7.0～7.5, and all the other are water, the ordinary method sterilization;
(c) cultivation in the seeding tank: above-mentioned cultured respectively level liquid kind is shaken up in the substratum of back seeding tank of mark 1%～5% inoculum size access by volume under aseptic condition, aerated culture 24～48h treats that the bacterium number reaches 1.0 * 10
9～5.0 * 10
9More than the CFU/mL, stop to cultivate;
(d) cultivation in the big fermentor tank: with above-mentioned cultured respectively lactic acid bacillus kind by volume the inoculum size of mark 10%～20% insert in the substratum of fermentor tank and cultivate, 37～42 ℃ of temperature, rotating speed 180～250r/min is by every m
3The air of substratum is 20～30m
3/ h carries out aerated culture 48～60h, begins to add sterilized glucose concentrated solution behind fermentation 16～40h, and total additional amount is a volume fraction 1%～4%, treats that the bacterium number reaches 1.0 * 10
9～5.0 * 10
9More than the CFU/mL, stop fermentation culture;
Described (c) and (d) substratum described in the step contain peptone 20.0～30.0g/L, yeast extract paste or extractum carnis or corn steep liquor 5.0～15g/L, glucose 10.0～40.0g/L, calcium salt 5.0～15.0g/L, sal epsom 0.1～0.5g/L, manganese salt 0.01～0.1g/L, all the other are water, pH 7.0～7.5, the ordinary method sterilization.
2. a multiple-effect microbial fodder additives is characterized in that described calcium salt is a lime carbonate, and described manganese salt is manganous sulfate.
3. according to claim 1,2 described a kind of multiple-effect microbial fodder additivess is characterized in that containing massfraction 2% ore powder, massfraction 2% zeolite powder, massfraction 2% wilkinite, massfraction 5% citric acid.
4. the preparation method of a multiple-effect microbial fodder additives is characterized in that comprising following several steps:
(a) slant strains activation: preservation inclined-plane kind lactic acid bacillus lined respectively on the inclined-plane kind substratum activate, contain peptone 10～20.0g/L in the described inclined-plane kind substratum, yeast extract paste or extractum carnis or corn steep liquor 3.0～10.0g/L, glucose 5～15.0g/L, agar 18～22g/L, all the other are water, and pH 7.0～7.2, the ordinary method sterilization;
(b) level liquid of bacterial classification activates: above-mentioned cultured inclined-plane kind is inoculated in respectively in the level liquid substratum with transfering loop cultivates, make the bacterium number of liquid strain reach 1.0 * 10
9～5.0 * 10
9More than the CFU/mL, contain peptone 15.0～25g/L in the described level liquid substratum, yeast extract paste or extractum carnis or corn steep liquor 5.0～15.0g/L, glucose 10.0～30.0g/L, calcium salt 5.0～15g/L, sal epsom 0.1～1.0g/L, manganese salt 0.01～0.1g/L, pH 7.0～7.5, and all the other are water, the ordinary method sterilization;
(c) cultivation in the seeding tank: above-mentioned cultured respectively level liquid kind is shaken up in the substratum of back seeding tank of mark 1%～5% inoculum size access by volume under aseptic condition, aerated culture 24～48h treats that the bacterium number reaches 1.0 * 10
9～5.0 * 10
9More than the CFU/mL, stop to cultivate;
(d) cultivation in the big fermentor tank: with above-mentioned cultured respectively lactic acid bacillus kind by volume the inoculum size of mark 10%～20% insert in the substratum of fermentor tank and cultivate, 37～42 ℃ of temperature, rotating speed 180～250r/min is 20～30m by the air of every m3 substratum
3/ h carries out aerated culture 48～60h, begins to add sterilized glucose concentrated solution behind fermentation 16～40h, and total additional amount is a volume fraction 1%～4%, treats that the bacterium number reaches 1.0 * 10
9～5.0 * 10
9More than the CFU/mL, stop fermentation culture;
Described (c) and (d) substratum described in the step contain peptone 20.0～30.0g/L, yeast extract paste or extractum carnis or corn steep liquor 5.0～15g/L, glucose 10.0～40.0g/L, calcium salt 5.0～15.0g/L, sal epsom 0.1～0.5g/L, manganese salt 0.01～0.1g/L, all the other are water, pH 7.0～7.5, the ordinary method sterilization.
5. the preparation method of a multiple-effect microbial fodder additives is characterized in that described calcium salt is a lime carbonate, and described manganese salt is manganous sulfate.
6. according to claim 4, the preparation method of 5 described a kind of multiple-effect microbial fodder additivess, it is characterized in that in the sophisticated fermented liquid that described (d) step produces, adding massfraction 2% ore powder, massfraction 2% zeolite powder, massfraction 2% wilkinite, massfraction 5% citric acid, stir, mix, promptly make the multiple-effect microbial fodder additives.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1957737B (en) * | 2005-10-31 | 2010-11-17 | 朱维奇 | Formula of health care feedstuff, and production method |
| CN101390571B (en) * | 2007-09-21 | 2011-03-23 | 北京大北农科技集团股份有限公司 | Coagulation bacillus feedstuff supplement and preparation method thereof |
| CN101548731B (en) * | 2008-05-14 | 2012-05-09 | 北京劲能生物科技股份公司 | Commercial pig green feed |
| CN102726606A (en) * | 2012-03-20 | 2012-10-17 | 重庆市畜牧科学院 | Micro-ecological synbiotic feed addictive, preparation method and application thereof |
| CN103849582A (en) * | 2013-12-30 | 2014-06-11 | 青岛碧水蓝天生物技术有限公司 | Lactobacillus strain with high productivity |
| CN105211632A (en) * | 2014-06-25 | 2016-01-06 | 都江堰惠农生物技术有限责任公司 | A kind of multiple-effect microbial feed addictive and preparation method thereof |
| CN105524855A (en) * | 2015-02-04 | 2016-04-27 | 江苏省苏微微生物研究有限公司 | Bacillus coagulans having aquatic pathogen antagonistic characteristic and application of bacillus coagulans |
| CN105795113A (en) * | 2016-04-12 | 2016-07-27 | 江苏益品实业有限公司 | Method for efficiently preparing bacillus coagulans pet food and application of bacillus coagulans pet food |
| CN107794241A (en) * | 2017-12-08 | 2018-03-13 | 亚太星原农牧科技海安有限公司 | One boar source lactic acid bacillus, probiotics and preparation method and application |
| CN114651915A (en) * | 2022-04-20 | 2022-06-24 | 湖北凯利特生物科技有限公司 | Metazoan capable of improving immunity and promoting growth, preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1957737B (en) * | 2005-10-31 | 2010-11-17 | 朱维奇 | Formula of health care feedstuff, and production method |
| CN101390571B (en) * | 2007-09-21 | 2011-03-23 | 北京大北农科技集团股份有限公司 | Coagulation bacillus feedstuff supplement and preparation method thereof |
| CN101548731B (en) * | 2008-05-14 | 2012-05-09 | 北京劲能生物科技股份公司 | Commercial pig green feed |
| CN102726606A (en) * | 2012-03-20 | 2012-10-17 | 重庆市畜牧科学院 | Micro-ecological synbiotic feed addictive, preparation method and application thereof |
| CN102726606B (en) * | 2012-03-20 | 2013-11-20 | 重庆市畜牧科学院 | Micro-ecological synbiotic feed addictive, preparation method and application thereof |
| CN103849582A (en) * | 2013-12-30 | 2014-06-11 | 青岛碧水蓝天生物技术有限公司 | Lactobacillus strain with high productivity |
| CN105211632A (en) * | 2014-06-25 | 2016-01-06 | 都江堰惠农生物技术有限责任公司 | A kind of multiple-effect microbial feed addictive and preparation method thereof |
| CN105524855A (en) * | 2015-02-04 | 2016-04-27 | 江苏省苏微微生物研究有限公司 | Bacillus coagulans having aquatic pathogen antagonistic characteristic and application of bacillus coagulans |
| CN105795113A (en) * | 2016-04-12 | 2016-07-27 | 江苏益品实业有限公司 | Method for efficiently preparing bacillus coagulans pet food and application of bacillus coagulans pet food |
| CN107794241A (en) * | 2017-12-08 | 2018-03-13 | 亚太星原农牧科技海安有限公司 | One boar source lactic acid bacillus, probiotics and preparation method and application |
| CN107794241B (en) * | 2017-12-08 | 2020-07-24 | 亚太星原农牧科技海安有限公司 | Porcine lactobacillus, microecological preparation, preparation method and application |
| CN114651915A (en) * | 2022-04-20 | 2022-06-24 | 湖北凯利特生物科技有限公司 | Metazoan capable of improving immunity and promoting growth, preparation method and application |
| CN114651915B (en) * | 2022-04-20 | 2023-12-19 | 湖北凯利特生物科技有限公司 | Method for preparing metazoan for improving immunity and promoting growth and application of metazoan |
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