CN103734089A - Artificial breeding method of predatory mites of phytoseiidae - Google Patents
Artificial breeding method of predatory mites of phytoseiidae Download PDFInfo
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- CN103734089A CN103734089A CN201310729519.9A CN201310729519A CN103734089A CN 103734089 A CN103734089 A CN 103734089A CN 201310729519 A CN201310729519 A CN 201310729519A CN 103734089 A CN103734089 A CN 103734089A
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- predatory mites
- phytoseiidae
- liquid feed
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Abstract
The invention discloses an artificial breeding method of predatory mites of phytoseiidae. The technological process of the artificial breeding method comprises preparing liquid feed, feeding the predatory mites after the liquid feed is packaged and breeding the predatory mites. The liquid feed is packaged into a small capsule through elongated package materials and placed into a breeding device and the predatory mites are added into the breeding device to be cultivated and bred. The liquid feed comprises 20 mg every mL of compound vitamin B5 and 20 mg every mL of vitamin C5 and also comprises the following components, by volume, 10 to 30% of egg yolk, 10 to 30% of milk, 2 to 10% of honey, 10 to 50% of fly maggot homogenate, 10 to 50% of flour mite homogenate, 0.5 to 3% of preservatives and the balance water. According to the artificial breeding method of the predatory mites of the phytoseiidae, the process operation is simple, the requirements for the breeding environment are low, the breeding effect on the predatory mites is good and the large-scale industrial production can be formed.
Description
Technical field
The invention belongs to the breeding technology field of Predatory Mites.More specifically, the rearing method that relates to a kind of Phytoseiidae Predatory Mites.
Background technology
At present, generally generation, the harm of farm-forestry crop evil mite have become worldwide problem, become the natural enemy of international joint research and the target that agricultural synthesis is administered.The control of China crops evil mite still be take chemical control as main, uses in a large number agricultural chemicals, causes evil mite pesticide resistance to increase severely, to such an extent as to the using dosage of agricultural chemicals increases or use high-toxic pesticide, seriously residue in crop, while contaminated environment, serious harm human body is healthy.
The biological control strategy of crops evil mite; especially the technology of " Using predatory mite to control pest mites " becomes the current optimal path of controlling in the world the harm of evil mite; be conducive to produce nuisanceless agricultural byproducts and preserve the ecological environment and bio-diversity, being conducive to the sustainable development of agricultural production.
China has realized the batch production of Predatory Mites and has produced, and its production technology mainly contains to be processed the early stage of the feeds such as crops leftovers, and flour mite is cultivated, and the Predatory Mites production of hybrid seeds is cultivated, and Predatory Mites such as cultivates in batches at the flow process.Whole technological process is many, specification requirement is high, labor intensive, has seriously restricted Predatory Mites applying in control of pest mite.
Summary of the invention
Technical problem to be solved by this invention is to overcome the technical deficiency that existing Predatory Mites is raised, especially the technical deficiency that Phytoseiidae Predatory Mites is raised, provides the Phytoseiidae Predatory Mites rearing method that a kind of technique is simple, low to feeding environment requirement, can form large-scale industrial production.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
The invention provides a kind of rearing method of Phytoseiidae Predatory Mites, the technological process of this rearing method comprises obtaining liq feed, will after liquid feed packing, for Predatory Mites, take food cultivation Predatory Mites.
Preferably, described Phytoseiidae Predatory Mites be amblyseius cucumeris oudeman (
neoseiulus cucumeris), Amblyseius barkeri (
amblyseius barkeri), Si Shi genus amblyseius berlese (
amblyseius swirskii) or Rui Shi genus amblyseius berlese (
neoseiulus swirskii).
More preferably, described Phytoseiidae Predatory Mites is amblyseius cucumeris oudeman or Amblyseius barkeri.
Preferably, described liquid feed packing is with lapping, liquid feed to be encapsulated, and makes folliculus, and the specification of folliculus is not done any restriction, in practical application, can determine according to situations such as feeding environment, the Predatory Mites numbers of animals raised.
Described lapping is that the mouthpart of Predatory Mites can thrust and material that liquid feed can not flow out.
Preferably, described lapping is stretchable sealed membrane or stretchable preservative film.
Described culture apparatus is through 75～90 ℃ of sterilizings.
Preferably, the condition of described cultivation is: 24～28 ℃ of temperature, relative moisture 60～90%.
More preferably, the condition of described cultivation is 25 ℃ of temperature, relative moisture 70%.
Preferably, described liquid feed comprises following component: vitamin B compound 5～20mg/mL, the component of vitamin C 5～20mg/mL and following volumn concentration: egg yolk 10%～30%, milk 10%～30%, honey 2%～10%, fly maggot homogenate 10%～50%, flour mite homogenate 10%～50% and preservative 0.5%～3%, surplus is water.
Preferably, described liquid feed comprises component vitamin B compound 5～15mg/mL, the component of vitamin C 5～15mg/mL and following volumn concentration: egg yolk 10%～20%, milk 10%～20%, honey 2%～5%, fly maggot homogenate 10%～20%, flour mite homogenate 10%～20% and preservative 0.5%～2%, surplus is water.
More preferably, described liquid feed comprises component vitamin B compound 5～10mg/mL, the component of vitamin C 5～10mg/mL and following volumn concentration: egg yolk 10%～15%, milk 10%～15%, honey 4%～5%, fly maggot homogenate 10%～15%, flour mite homogenate 10%～15% and preservative 0.5%～1%, surplus is water.
Preferably, described milk is commercially available plain chocolate product, commercially available fresh milk product or through the fresh milk of the firm output of milk cow of sterilizing.
Described fly maggot homogenate is that fly maggot larva is added to water, carries out homogenate, and homogenate is centrifugal, gets supernatant and obtains fly maggot homogenate; Described flour mite homogenate is to get flour mite to add water, carries out homogenate, and homogenate is centrifugal, gets supernatant and obtains flour mite homogenate.
Preferably, described preservative is one or more the combination in potassium sorbate, Sodium Benzoate, streptococcus lactis, natamycin, dehydro sodium acetate, propylparaben or dehydroacetic acid; While being used in combination, mixed proportion is not done strict restriction.
More preferably, described preservative is one or more the combination in potassium sorbate, Sodium Benzoate or propylparaben; While being used in combination, mixed proportion is not done strict restriction.
Preferably, the compound method of described liquid feed is as follows:
S1. fly maggot larva is added to water, carry out homogenate, homogenate is centrifugal, gets supernatant standby;
S2. get flour mite and add water, carry out homogenate, homogenate is centrifugal, gets supernatant standby;
S3. take in proportion egg yolk, milk and honey, standby;
S4. vitamin B compound and vitamin C are pulverized respectively, standby;
S5. by ready each component of S1～S4, mix, add preservative, surplus water polishing, stirs, and has both obtained liquid feed.
As a kind of preferably, in the component of liquid feed of the present invention and prepare described liquid feed water used and be sterile water; Aseptic condition is more conducive to the long-term preservation of liquid feed.
The present invention has following beneficial effect:
The invention provides a kind of rearing method of Phytoseiidae Predatory Mites, the ecological conditions such as liquid feed, temperature and humidity required in artificial feeding process draw through large quantity research, adopt aforesaid liquid feed and raising method, can successfully breed Predatory Mites, and feeding effect is very good.The Predatory Mites of raising, for biological control, can significantly improve output and the quality of crop, reduces residue of pesticide, is conducive to preserve the ecological environment.
In addition, the present invention makes liquid feed the form of folliculus for raising Predatory Mites, during Predatory Mites feed, the surface that can initiatively be attached to folliculus takes food, increased the surface area that takes food of the interior Predatory Mites of unit volume, not only can save space, can also effectively prevent the volatilization of liquid feed, further reduce costs.
The rearing method technique of Phytoseiidae Predatory Mites of the present invention is simple, and cost is low, low to feeding environment requirement, and feeding environment is cleaner, is highly suitable for the large-scale promotion application of industrialization.
Embodiment
Below in conjunction with specific embodiment, further illustrate the present invention, but embodiment does not limit in any form to the present invention.Unless stated otherwise, reagent, the method and apparatus that the present invention adopts is the conventional reagent of the art, method and apparatus.
embodiment 1
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added to the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 10ml supernatant standby;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 10ml supernatant standby;
S3. get egg yolk 15ml standby;
S4. get milk 15ml standby;
S5. get honey 5ml standby;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1g and be placed in container;
S7. ready each component of S1～S6 is mixed in the container of S6, adds 1ml potassium sorbate, surplus, stirs to 100ml with sterile water polishing.
2, the lapping elongating for liquid feed (described lapping is stretchable sealed membrane or stretchable preservative film, following embodiment with) is made to folliculus, the specification of folliculus is roughly 1～2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access Amblyseius barkeri (
amblyseius barkeri) cultivate and breed.The condition that cultivation is bred is 25 ℃ of temperature, relative moisture 70%.
Result demonstration, raises about 10 days, and Amblyseius barkeri can expand rapidly numerous, and the amount of Amblyseius barkeri approximately increases weekly 2～10 times.
embodiment 2
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added to the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 13ml supernatant standby;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 10ml sterile water, then that homogenate is centrifugal, get 13ml supernatant standby;
S3. get egg yolk 12ml standby;
S4. get milk 12ml standby;
S5. get honey 5ml standby;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 0.8g and be placed in container;
S7. ready each component of S1～S6 is mixed in the container of S6, adds 0.8ml Sodium Benzoate, surplus, stirs to 100ml with sterile water polishing.
2, liquid feed is made to folliculus with the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongating, the specification of folliculus is roughly 1～2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access amblyseius cucumeris oudeman (
neoseiulus cucumeris) cultivate and breed.The condition that cultivation is bred is 26 ℃ of temperature, relative moisture 80%.
Result demonstration, raises about 10 days, and amblyseius cucumeris oudeman can expand rapidly numerous, and the amount of amblyseius cucumeris oudeman approximately increases weekly 2～10 times.
embodiment 3
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added to the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 11ml supernatant standby;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 10ml sterile water, then that homogenate is centrifugal, get 11ml supernatant standby;
S3. get egg yolk 14ml standby;
S4. get milk 14ml standby;
S5. get honey 4ml standby;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 0.5g and be placed in container;
S7. ready each component of S1～S6 is mixed in the container of S6, adds 1ml propylparaben, surplus, stirs to 100ml with sterile water polishing.
2, liquid feed is made to folliculus with the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongating, the specification of folliculus is roughly 1～2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access Amblyseius barkeri is cultivated and is bred.The condition that cultivation is bred is 27 ℃ of temperature, relative moisture 70%.
Result demonstration, raises about 10 days, and Amblyseius barkeri can expand rapidly numerous, and the amount of Amblyseius barkeri approximately increases weekly 2～10 times.
embodiment 4
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added to the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 12ml supernatant standby;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 13ml sterile water, then that homogenate is centrifugal, get 15ml supernatant standby;
S3. get egg yolk 15ml standby;
S4. get milk 15ml standby;
S5. get honey 5ml standby;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1.5g and be placed in container;
S7. ready each component of S1～S6 is mixed in the container of S6, adds 2ml Sodium Benzoate, surplus, stirs to 100ml with sterile water polishing.
2, liquid feed is made to folliculus with the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongating, the specification of folliculus is roughly 1～2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access amblyseius cucumeris oudeman is cultivated and is bred.The condition that cultivation is bred is 28 ℃ of temperature, relative moisture 70%.
Result demonstration, raises about 10 days, and amblyseius cucumeris oudeman can expand rapidly numerous, and the amount of amblyseius cucumeris oudeman approximately increases weekly 2～10 times.
embodiment 5
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added to the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 13ml supernatant standby;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 13ml supernatant standby;
S3. get egg yolk 10ml standby;
S4. get milk 10ml standby;
S5. get honey 5ml standby;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1.5g and be placed in container;
S7. ready each component of S1～S6 is mixed in the container of S6, adds 2.5ml propylparaben, surplus, stirs to 100ml with sterile water polishing.
2, liquid feed is made to folliculus with the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongating, the specification of folliculus is roughly 1～2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access Rui Shi genus amblyseius berlese (
neoseiulus swirskii) cultivate and breed.The condition that cultivation is bred is 24 ℃ of temperature, relative moisture 70%.
Result demonstration, raises about 10 days, and Rui Shi genus amblyseius berlese can expand rapidly numerous, and the amount of Rui Shi genus amblyseius berlese approximately increases weekly 2～10 times.
embodiment 6
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added to the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 10ml supernatant standby;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 15ml supernatant standby;
S3. get egg yolk 11ml standby;
S4. get milk 11ml standby;
S5. get honey 5ml standby;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1g and be placed in container;
S7. ready each component of S1～S6 is mixed in the container of S6, adds 1ml dehydro sodium acetate, surplus, stirs to 100ml with sterile water polishing.
2, liquid feed is made to folliculus with the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongating, the specification of folliculus is roughly 1～2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access Si Shi genus amblyseius berlese (
amblyseius swirskii) cultivate and breed.The condition that cultivation is bred is 25 ℃ of temperature, relative moisture 60%.
Result demonstration, raises about 10 days, and Si Shi genus amblyseius berlese can expand rapidly numerous, and the amount of Si Shi genus amblyseius berlese approximately increases weekly 2～10 times.
Claims (10)
1. a rearing method for Phytoseiidae Predatory Mites, is characterized in that, the technological process of this rearing method comprises obtaining liq feed, will after liquid feed packing, for Predatory Mites, take food cultivation Predatory Mites.
2. the rearing method of Phytoseiidae Predatory Mites according to claim 1, is characterized in that, described Phytoseiidae Predatory Mites is amblyseius cucumeris oudeman, Amblyseius barkeri, Si Shi genus amblyseius berlese or Rui Shi genus amblyseius berlese.
3. the rearing method of Phytoseiidae Predatory Mites according to claim 1, is characterized in that, described Phytoseiidae Predatory Mites is amblyseius cucumeris oudeman or Amblyseius barkeri.
4. the rearing method of Phytoseiidae Predatory Mites according to claim 1, is characterized in that, described liquid feed packing is with lapping, liquid feed to be encapsulated, and makes folliculus.
5. the rearing method of Phytoseiidae Predatory Mites according to claim 1, is characterized in that, the cultivation of described Predatory Mites is that packaged folliculus is placed in culture apparatus, accesses Predatory Mites in culture apparatus, cultivates.
6. the rearing method of Phytoseiidae Predatory Mites according to claim 1 or 5, is characterized in that, the condition of described cultivation is 24～28 ℃ of temperature, relative moisture 60～90%.
7. the rearing method of Phytoseiidae Predatory Mites according to claim 1 or 5, is characterized in that, the condition of described cultivation is 25 ℃ of temperature, relative moisture 70%.
8. the rearing method of Phytoseiidae Predatory Mites according to claim 4, is characterized in that, the mouthpart that described lapping is Predatory Mites can thrust and material that liquid feed can not flow out.
9. the rearing method of Phytoseiidae Predatory Mites according to claim 4, is characterized in that, described lapping is stretchable sealed membrane or stretchable preservative film.
10. the rearing method of Phytoseiidae Predatory Mites according to claim 5, is characterized in that, described culture apparatus is through 75～90 ℃ of sterilizings.
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Cited By (5)
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| CN104222661A (en) * | 2014-09-26 | 2014-12-24 | 张可池 | Nutrition-supplementing additive for predatory mite |
| CN105746254A (en) * | 2016-03-14 | 2016-07-13 | 福建艳璇生物防治技术有限公司 | Method for preventing red spiders, thrips and aleyrodids on strawberries by amblyseius cucumeris |
| CN107361027A (en) * | 2017-08-31 | 2017-11-21 | 中国农业科学院植物保护研究所 | A kind of device and method using man-made feeds circulation sterilization raising predatism stinkbug |
| CN107432388A (en) * | 2017-07-24 | 2017-12-05 | 广西南亚热带农业科学研究所 | A kind of cassava nutrient solution of efficiently breeding Tetranychus cinnabarinus |
| CN107927356A (en) * | 2017-12-06 | 2018-04-20 | 中国农业科学院植物保护研究所 | A kind of gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation |
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| CN107432388A (en) * | 2017-07-24 | 2017-12-05 | 广西南亚热带农业科学研究所 | A kind of cassava nutrient solution of efficiently breeding Tetranychus cinnabarinus |
| CN107432388B (en) * | 2017-07-24 | 2020-08-14 | 广西南亚热带农业科学研究所 | Cassava nutrient solution for efficiently propagating tetranychus cinnabarinus |
| CN107361027A (en) * | 2017-08-31 | 2017-11-21 | 中国农业科学院植物保护研究所 | A kind of device and method using man-made feeds circulation sterilization raising predatism stinkbug |
| CN107927356A (en) * | 2017-12-06 | 2018-04-20 | 中国农业科学院植物保护研究所 | A kind of gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation |
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