CN103750016B - A kind of liquid feed for raising Phytoseiidae Predatory Mites and preparation method thereof - Google Patents
A kind of liquid feed for raising Phytoseiidae Predatory Mites and preparation method thereof Download PDFInfo
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- CN103750016B CN103750016B CN201310728678.7A CN201310728678A CN103750016B CN 103750016 B CN103750016 B CN 103750016B CN 201310728678 A CN201310728678 A CN 201310728678A CN 103750016 B CN103750016 B CN 103750016B
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Abstract
The invention discloses a kind of liquid feed for raising Phytoseiidae Predatory Mites and preparation method thereof.Does described liquid feed comprise vitamin B compound? 5 ~ 20mg/mL, vitamin C? the component of 5 ~ 20mg/mL and following volumn concentration: egg yolk 10% ~ 30%, milk 10% ~ 30%, honey 2% ~ 10%, fly maggot homogenate 10% ~ 50%, flour mite homogenate 10% ~ 50% and anticorrisive agent 0.5% ~ 3%, surplus is water.After vitamin B compound and vitamin C according to dosage being ground respectively, add other materials above-mentioned mixing, surplus water polishing, stirs, and obtains described liquid feed.Liquid feed of the present invention makes simple, and cost is low, can meet the nutritional need of Phytoseiidae Predatory Mites completely, and feeding effect is fine, can be used for the large-scale industrial production of Predatory Mites.
Description
Technical field
The invention belongs to the breeding technology field of Predatory Mites.More specifically, a kind of liquid feed for raising Phytoseiidae Predatory Mites and preparation method thereof is related to.
Background technology
Generally generation, the harm that runs wild of farm-forestry crop evil mite (comprising red spider, blister mite, tarsonemid, flour mite) become worldwide problem.Small worm becomes terrible disaster Yi Shi China development trend of insect in agricultural and forestry production in recent years, such as, red spider evil mite on cotton rises to primary pest, wheat red mite harm wheat yield, tarsonemid hazard rice causes the underproduction, citrus red mite, blister mite harm citrus, have a strong impact on the yield and quality of citrus.The edible mushrooms such as the harm such as flour mite, Pu mite, tarsonemid mushroom, white fungus, mushroom, cause the crushing loss such as rotten bed, rotten kind; In addition, mite class harm city trees and shrubs all in various degree, flowers, tea place, booth vegetable.
Current China crops evil mite is still based on chemical prevention.A large amount of uses of agricultural chemicals, cause the evil mite resistance to the action of a drug to increase severely, force the using dosage of agricultural chemicals increase or use high-toxic pesticide, residual serious in crop, contaminated environment, serious harm human body is healthy.Use biological control strategy, especially " using predatory mite to control pest mites " technology becomes the current optimal path controlling the harm of evil mite in the world.
The raising of the Predatory Mites such as amblyseius cucumeris oudeman mainly relies on agricultural byproduct to cultivate flour mite, and take flour mite as food and then cultivation Predatory Mites, whole raising flow process is many, take up room large, labor intensive, seriously constrains Predatory Mites applying in control of pest mite.
Summary of the invention
The technical problem to be solved in the present invention is the technical deficiency overcoming the raising of existing Predatory Mites, there is provided a kind of, liquid feed of can be used for large-scale industrial production Predatory Mites low to feeding environment requirement, in particular for raising the liquid feed of Phytoseiidae Predatory Mites.
Another object of the present invention is to provide described for raising the preparation method of the liquid feed of Phytoseiidae Predatory Mites.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
The invention provides a kind of liquid feed for raising Phytoseiidae Predatory Mites, described liquid feed comprises component vitamin B compound 5 ~ 20mg/mL, the component of vitamin C 5 ~ 20mg/mL and following volumn concentration: egg yolk 10% ~ 30%, milk 10% ~ 30%, honey 2% ~ 10%, fly maggot homogenate 10% ~ 50%, flour mite homogenate 10% ~ 50% and anticorrisive agent, 0.5% ~ 3%, surplus is water.
Preferably, described liquid feed comprises component vitamin B compound 5 ~ 15mg/mL, the component of vitamin C 5 ~ 15mg/mL and following volumn concentration: egg yolk 10% ~ 20%, milk 10% ~ 20%, honey 2% ~ 5%, fly maggot homogenate 10% ~ 20%, flour mite homogenate 10% ~ 20% and anticorrisive agent 0.5% ~ 2%, surplus is water.
More preferably, described liquid feed comprises component vitamin B compound 5 ~ 10mg/mL, the component of vitamin C 5 ~ 10mg/mL and following volumn concentration: egg yolk 10% ~ 15%, milk 10% ~ 15%, honey 4% ~ 5%, fly maggot homogenate 10% ~ 15%, flour mite homogenate 10% ~ 15% and anticorrisive agent 0.5% ~ 1%, surplus is water.
Preferably, described milk is the fresh milk of commercially available plain chocolate product, commercially available fresh milk product or the firm output of milk cow through sterilizing.
Described fly maggot homogenate is that fly maggot larva is added water, carries out homogenate, and homogenate is centrifugal, gets supernatant and namely obtains fly maggot homogenate; Described flour mite homogenate gets flour mite to add water, carries out homogenate, and homogenate is centrifugal, gets supernatant and namely obtain flour mite homogenate.
Preferably, described Phytoseiidae Predatory Mites be amblyseius cucumeris oudeman (
neoseiuluscucumeris), Amblyseius barkeri (
amblyseiusbarkeri), Si Shi amblyseius as predatory mite (
amblyseiusswirskii) or Rui Shi amblyseius as predatory mite (
neoseiulusswirskii).
More preferably, described Phytoseiidae Predatory Mites is amblyseius cucumeris oudeman or Amblyseius barkeri.
Preferably, described anticorrisive agent is the combination of one or more in potassium sorbate, Sodium Benzoate, streptococcus lactis, natamycin, dehydro sodium acetate, propylparaben or dehydroacetic acid; When combinationally using, mixed proportion does not do considered critical.
More preferably, described anticorrisive agent is the combination of one or more in potassium sorbate, Sodium Benzoate or propylparaben; When combinationally using, mixed proportion does not do considered critical.
Present invention also offers a kind of described for raising the preparation method of the liquid feed of Phytoseiidae Predatory Mites, step is as follows:
S1. fly maggot larva is added water, carry out homogenate, homogenate is centrifugal, gets supernatant for subsequent use;
Get flour mite and add water, carry out homogenate, homogenate is centrifugal, gets supernatant for subsequent use;
Take egg yolk, milk and honey in proportion, for subsequent use;
Vitamin B compound and vitamin C are pulverized respectively, for subsequent use;
S2. by ready for S1 each component, mix, add anticorrisive agent, surplus water polishing, stirs, and both obtains liquid feed.
Preferred as one, in the component of liquid feed of the present invention and the water preparing described liquid feed used be sterilized water; Aseptic condition is more conducive to the long-term preservation of liquid feed.
The present invention has following beneficial effect:
The invention solves technical problem and the deficiencies such as the flow process that the Predatory Mites artificial diet technique such as amblyseius cucumeris oudeman exists is many, large, the labor intensive of taking up room, provide a kind of liquid feed for raising Phytoseiidae Predatory Mites, and this feedstuff is easily looked for, cost is low, making is simple, and nutrition is more rationally abundant.
Liquid feed of the present invention for raising Predatory Mites, can normal spawning, hatching reaching maturity, feeding effect is good; And it is little to take up room, feeding environment is cleaner, is applicable to the large-scale industrial production of the Predatory Mites such as amblyseius cucumeris oudeman or Amblyseius barkeri.
Detailed description of the invention
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment does not limit in any form to the present invention.Unless stated otherwise, the present invention adopts reagent, method and apparatus are the art conventional reagent, method and apparatus.
embodiment 1
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterilized water, then that homogenate is centrifugal, get 10ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 15ml sterilized water, then that homogenate is centrifugal, get 10ml supernatant for subsequent use;
S3. egg yolk 15ml is got for subsequent use;
S4. milk 15ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 1ml potassium sorbate, surplus, stirs to 100ml with sterilized water polishing.
2, (described lapping is stretchable sealed membrane or stretchable preservative film to the lapping elongated by liquid feed, following embodiment is same) (specification of folliculus is roughly 1 ~ 2cm to make folliculus, but therefore do not limit the present invention) after, be placed in culture apparatus, access Amblyseius barkeri (
amblyseiusbarkeri) carry out cultivation and breed.Cultivating the condition of breeding is temperature 25 DEG C, relative humidity 70%.
Result shows, and raise about 10 days, Amblyseius barkeri can expand rapidly numerous, and the amount of Amblyseius barkeri approximately increases by 2 ~ 10 times weekly.
embodiment 2
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterilized water, then that homogenate is centrifugal, get 13ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 10ml sterilized water, then that homogenate is centrifugal, get 13ml supernatant for subsequent use;
S3. egg yolk 12ml is got for subsequent use;
S4. milk 12ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 0.8g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 0.8ml Sodium Benzoate, surplus, stirs to 100ml with sterilized water polishing.
2, (specification of folliculus is roughly 1 ~ 2cm to the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongated by liquid feed to make folliculus, but therefore do not limit the present invention) after, be placed in culture apparatus, access amblyseius cucumeris oudeman (
neoseiuluscucumeris) carry out cultivation and breed.Cultivating the condition of breeding is temperature 26 DEG C, relative humidity 80%.
Result shows, and raise about 10 days, amblyseius cucumeris oudeman can expand rapidly numerous, and the amount of amblyseius cucumeris oudeman approximately increases by 2 ~ 10 times weekly.
embodiment 3
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterilized water, then that homogenate is centrifugal, get 11ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 10ml sterilized water, then that homogenate is centrifugal, get 11ml supernatant for subsequent use;
S3. egg yolk 14ml is got for subsequent use;
S4. milk 14ml is got for subsequent use;
S5. honey 4ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 0.5g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 1ml propylparaben, surplus, stirs to 100ml with sterilized water polishing.
2, (specification of folliculus is roughly 1 ~ 2cm to the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongated by liquid feed to make folliculus, but therefore do not limit the present invention) after, be placed in culture apparatus, access Amblyseius barkeri carries out cultivation and breeds.Cultivating the condition of breeding is temperature 27 DEG C, relative humidity 70%.
Result shows, and raise about 10 days, Amblyseius barkeri can expand rapidly numerous, and the amount of Amblyseius barkeri approximately increases by 2 ~ 10 times weekly.
embodiment 4
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterilized water, then that homogenate is centrifugal, get 12ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 13ml sterilized water, then that homogenate is centrifugal, get 15ml supernatant for subsequent use;
S3. egg yolk 15ml is got for subsequent use;
S4. milk 15ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1.5g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 2ml Sodium Benzoate, surplus, stirs to 100ml with sterilized water polishing.
2, (specification of folliculus is roughly 1 ~ 2cm to the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongated by liquid feed to make folliculus, but therefore do not limit the present invention) after, be placed in culture apparatus, access amblyseius cucumeris oudeman carries out cultivation and breeds.Cultivating the condition of breeding is temperature 28 DEG C, relative humidity 70%.
Result shows, and raise about 10 days, amblyseius cucumeris oudeman can expand rapidly numerous, and the amount of amblyseius cucumeris oudeman approximately increases by 2 ~ 10 times weekly.
embodiment 5
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterilized water, then that homogenate is centrifugal, get 13ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 15ml sterilized water, then that homogenate is centrifugal, get 13ml supernatant for subsequent use;
S3. egg yolk 10ml is got for subsequent use;
S4. milk 10ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1.5g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 2.5ml propylparaben, surplus, stirs to 100ml with sterilized water polishing.
2, (specification of folliculus is roughly 1 ~ 2cm to the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongated by liquid feed to make folliculus, but therefore do not limit the present invention) after, be placed in culture apparatus, access Rui Shi amblyseius as predatory mite (
neoseiulusswirskii) carry out cultivation and breed.Cultivating the condition of breeding is temperature 24 DEG C, relative humidity 70%.
Result shows, and raise about 10 days, Rui Shi amblyseius as predatory mite can expand rapidly numerous, and the amount of Rui Shi amblyseius as predatory mite approximately increases by 2 ~ 10 times weekly.
embodiment 6
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterilized water, then that homogenate is centrifugal, get 10ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 15ml sterilized water, then that homogenate is centrifugal, get 15ml supernatant for subsequent use;
S3. egg yolk 11ml is got for subsequent use;
S4. milk 11ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 1ml dehydro sodium acetate, surplus, stirs to 100ml with sterilized water polishing.
2, (specification of folliculus is roughly 1 ~ 2cm to the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) elongated by liquid feed to make folliculus, but therefore do not limit the present invention) after, be placed in culture apparatus, access Si Shi amblyseius as predatory mite (
amblyseiusswirskii) carry out cultivation and breed.Cultivating the condition of breeding is temperature 25 DEG C, relative humidity 60%.
Result shows, and raise about 10 days, Si Shi amblyseius as predatory mite can expand rapidly numerous, and the amount of Si Shi amblyseius as predatory mite approximately increases by 2 ~ 10 times weekly.
Claims (5)
1. one kind for raising the liquid feed of Phytoseiidae Predatory Mites, it is characterized in that, described liquid feed comprises component vitamin B compound 5 ~ 15mg/mL, the component of vitamin C 5 ~ 15mg/mL and following volumn concentration: egg yolk 10% ~ 20%, milk 10% ~ 20%, honey 2% ~ 5%, fly maggot homogenate 10% ~ 20%, flour mite homogenate 10% ~ 20% and anticorrisive agent 0.5% ~ 2%, surplus is water;
Described fly maggot homogenate is that fly maggot larva is added water, carries out homogenate, and homogenate is centrifugal, gets supernatant and namely obtains fly maggot homogenate; Described flour mite homogenate gets flour mite to add water, carries out homogenate, and homogenate is centrifugal, gets supernatant and namely obtain flour mite homogenate;
Described Phytoseiidae Predatory Mites is amblyseius cucumeris oudeman, Amblyseius barkeri, Si Shi amblyseius as predatory mite or Rui Shi amblyseius as predatory mite;
Described anticorrisive agent is the combination of one or more in potassium sorbate, Sodium Benzoate, streptococcus lactis, natamycin, dehydro sodium acetate, propylparaben or dehydroacetic acid.
2. the liquid feed for raising Phytoseiidae Predatory Mites according to claim 1, it is characterized in that, described liquid feed comprises component vitamin B compound 5 ~ 10mg/mL, the component of vitamin C 5 ~ 10mg/mL and following volumn concentration: egg yolk 10% ~ 15%, milk 10% ~ 15%, honey 4% ~ 5%, fly maggot homogenate 10% ~ 15%, flour mite homogenate 10% ~ 15% and anticorrisive agent 0.5% ~ 1%, surplus is water.
3. the liquid feed for raising Phytoseiidae Predatory Mites according to claim 1 and 2, is characterized in that, described Phytoseiidae Predatory Mites is amblyseius cucumeris oudeman or Amblyseius barkeri.
4. the liquid feed for raising Phytoseiidae Predatory Mites according to claim 1 and 2, is characterized in that, described anticorrisive agent is the combination of one or more in potassium sorbate, Sodium Benzoate or propylparaben.
5. according to claim 1 or 2 for raising the liquid feed of Phytoseiidae Predatory Mites, it is characterized in that, the preparation method of this liquid feed comprises the steps:
S1. fly maggot larva is added water, carry out homogenate, homogenate is centrifugal, gets supernatant for subsequent use;
Get flour mite and add water, carry out homogenate, homogenate is centrifugal, gets supernatant for subsequent use;
Take egg yolk, milk and honey in proportion, for subsequent use;
Vitamin B compound and vitamin C are pulverized respectively, for subsequent use;
S2. by ready for S1 each component, mix, add anticorrisive agent, surplus water polishing, stirs, obtains liquid feed.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108486198A (en) * | 2018-03-13 | 2018-09-04 | 佛山汇沐化学科技有限公司 | A kind of extracting method of pillworm polypeptide and its application |
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|---|---|---|---|---|
| CN104222661A (en) * | 2014-09-26 | 2014-12-24 | 张可池 | Nutrition-supplementing additive for predatory mite |
| CN104770573A (en) * | 2015-02-07 | 2015-07-15 | 广州大学 | Application of natamycin in preparation of liquid feed |
| CN109699834A (en) * | 2017-10-25 | 2019-05-03 | 石河子大学 | A kind of man-made feeds of the new small peaceful mite of double tails |
| CN107927356B (en) * | 2017-12-06 | 2021-07-20 | 中国农业科学院植物保护研究所 | Colloidal artificial improved feed for indoor propagation of amblyseius cucumeris population |
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