CN103734089B - A kind of rearing method of Phytoseiidae Predatory Mites - Google Patents
A kind of rearing method of Phytoseiidae Predatory Mites Download PDFInfo
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- CN103734089B CN103734089B CN201310729519.9A CN201310729519A CN103734089B CN 103734089 B CN103734089 B CN 103734089B CN 201310729519 A CN201310729519 A CN 201310729519A CN 103734089 B CN103734089 B CN 103734089B
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- 241000238876 Acari Species 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 34
- 241001481699 Phytoseiidae Species 0.000 title claims abstract description 15
- 230000000384 rearing effect Effects 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 24
- 235000013312 flour Nutrition 0.000 claims abstract description 19
- 239000003755 preservative agent Substances 0.000 claims abstract description 17
- 230000002335 preservative effect Effects 0.000 claims abstract description 17
- 235000013336 milk Nutrition 0.000 claims abstract description 16
- 210000004080 milk Anatomy 0.000 claims abstract description 16
- 239000008267 milk Substances 0.000 claims abstract description 16
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 12
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 12
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 12
- 229930003270 Vitamin B Natural products 0.000 claims abstract description 12
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 12
- 235000013345 egg yolk Nutrition 0.000 claims abstract description 12
- 210000002969 egg yolk Anatomy 0.000 claims abstract description 12
- 235000012907 honey Nutrition 0.000 claims abstract description 12
- 235000019156 vitamin B Nutrition 0.000 claims abstract description 12
- 239000011720 vitamin B Substances 0.000 claims abstract description 12
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 12
- 239000011718 vitamin C Substances 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 8
- 239000006228 supernatant Substances 0.000 claims description 18
- 241001206567 Neoseiulus barkeri Species 0.000 claims description 11
- 241001455175 Neoseiulus cucumeris Species 0.000 claims description 11
- 241000406588 Amblyseius Species 0.000 claims description 10
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 claims description 10
- 239000012528 membrane Substances 0.000 claims description 8
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 claims description 5
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 claims description 5
- 229960003415 propylparaben Drugs 0.000 claims description 5
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 5
- 239000004299 sodium benzoate Substances 0.000 claims description 5
- 235000010234 sodium benzoate Nutrition 0.000 claims description 5
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 4
- 229940125898 compound 5 Drugs 0.000 claims description 4
- 239000004302 potassium sorbate Substances 0.000 claims description 4
- 235000010241 potassium sorbate Nutrition 0.000 claims description 4
- 229940069338 potassium sorbate Drugs 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 239000004287 Dehydroacetic acid Substances 0.000 claims description 2
- 241000194035 Lactococcus lactis Species 0.000 claims description 2
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 2
- 235000019258 dehydroacetic acid Nutrition 0.000 claims description 2
- JEQRBTDTEKWZBW-UHFFFAOYSA-N dehydroacetic acid Chemical compound CC(=O)C1=C(O)OC(C)=CC1=O JEQRBTDTEKWZBW-UHFFFAOYSA-N 0.000 claims description 2
- 229940061632 dehydroacetic acid Drugs 0.000 claims description 2
- PGRHXDWITVMQBC-UHFFFAOYSA-N dehydroacetic acid Natural products CC(=O)C1C(=O)OC(C)=CC1=O PGRHXDWITVMQBC-UHFFFAOYSA-N 0.000 claims description 2
- 239000004311 natamycin Substances 0.000 claims description 2
- 235000010298 natamycin Nutrition 0.000 claims description 2
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 claims description 2
- 229960003255 natamycin Drugs 0.000 claims description 2
- 229960004249 sodium acetate Drugs 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 abstract description 15
- 235000013305 food Nutrition 0.000 abstract description 4
- 238000004806 packaging method and process Methods 0.000 abstract description 3
- 230000003031 feeding effect Effects 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract description 2
- 239000008223 sterile water Substances 0.000 description 19
- 238000009395 breeding Methods 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 7
- 238000005498 polishing Methods 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 230000003442 weekly effect Effects 0.000 description 6
- 241001664794 Amblyseius swirskii Species 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
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- 241001465754 Metazoa Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
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- 239000006227 byproduct Substances 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
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- 229960003885 sodium benzoate Drugs 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of rearing method of Phytoseiidae Predatory Mites.The technological process of this rearing method comprises obtaining liq feed, takes food after liquid feed packaging for Predatory Mites, cultivates Predatory Mites.The lapping that liquid feed elongates is packaged into folliculus by the present invention, is placed in culture apparatus, accesses Predatory Mites and carry out cultivation and breed in culture apparatus.Does described liquid feed comprise vitamin B compound? 5 ~ 20mg/mL, vitamin C? the component of 5 ~ 20mg/mL and following volumn concentration: egg yolk 10% ~ 30%, milk 10% ~ 30%, honey 2% ~ 10%, fly maggot homogenate 10% ~ 50%, flour mite homogenate 10% ~ 50% and preservative 0.5% ~ 3%, surplus is water.Present invention process is simple to operate, requires low to feeding environment, fine to the feeding effect of Predatory Mites, can form large-scale industrial production.
Description
Technical field
The invention belongs to the breeding technology field of Predatory Mites.More specifically, a kind of rearing method of Phytoseiidae Predatory Mites is related to.
Background technology
At present, generally generation, the harm of farm-forestry crop evil mite become worldwide problem, become the target that the natural enemy of international joint research and agricultural synthesis are administered.The control of China crops evil mite, still based on chemical control, uses agricultural chemicals in a large number, causes evil mite pesticide resistance to increase severely, to such an extent as to the using dosage of agricultural chemicals increases or uses high-toxic pesticide, seriously residue in crop, simultaneously contaminated environment, serious harm human body is healthy.
The biological control strategy of crops evil mite; especially the technology of " using predatory mite to control pest mites " becomes the current optimal path controlling the harm of evil mite in the world; be conducive to producing nuisanceless agricultural byproducts and preserving the ecological environment and bio-diversity, be conducive to the sustainable development of agricultural production.
The flow processs such as China has achieved the factorial praluction of Predatory Mites, and its production technology mainly contains the process in early stage of the feeds such as crops leftovers, and flour mite is cultivated, and the Predatory Mites production of hybrid seeds is cultivated, the cultivation of Predatory Mites batch.Whole technological process is many, technical requirement is high, labor intensive, seriously constrains Predatory Mites applying in control of pest mite.
Summary of the invention
Technical problem to be solved by this invention is the technical deficiency overcoming the raising of existing Predatory Mites, especially the technical deficiency of Phytoseiidae Predatory Mites raising, provides the Phytoseiidae Predatory Mites rearing method that a kind of technique is simple, low to feeding environment requirement, can form large-scale industrial production.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
The invention provides a kind of rearing method of Phytoseiidae Predatory Mites, the technological process of this rearing method comprises obtaining liq feed, takes food after liquid feed packaging for Predatory Mites, cultivates Predatory Mites.
Preferably, described Phytoseiidae Predatory Mites be amblyseius cucumeris oudeman (
neoseiuluscucumeris), Amblyseius barkeri (
amblyseiusbarkeri), Si Shi genus amblyseius berlese (
amblyseiusswirskii) or Rui Shi genus amblyseius berlese (
neoseiulusswirskii).
More preferably, described Phytoseiidae Predatory Mites is amblyseius cucumeris oudeman or Amblyseius barkeri.
Preferably, described liquid feed packaging is encapsulated by liquid feed with lapping, and make folliculus, the specification of folliculus does not do any restriction, can determine in practical application according to situations such as feeding environment, the Predatory Mites numbers of animals raised.
Described lapping is that the mouthpart of Predatory Mites can thrust and the material that liquid feed can not flow out.
Preferably, described lapping is stretchable sealed membrane or stretchable preservative film.
Described culture apparatus is through 75 ~ 90 DEG C of sterilizings.
Preferably, the condition of described cultivation is: temperature 24 ~ 28 DEG C, relative moisture 60 ~ 90%.
More preferably, the condition of described cultivation is temperature 25 DEG C, relative moisture 70%.
Preferably, described liquid feed comprises following component: vitamin B compound 5 ~ 20mg/mL, the component of vitamin C 5 ~ 20mg/mL and following volumn concentration: egg yolk 10% ~ 30%, milk 10% ~ 30%, honey 2% ~ 10%, fly maggot homogenate 10% ~ 50%, flour mite homogenate 10% ~ 50% and preservative 0.5% ~ 3%, surplus is water.
Preferably, described liquid feed comprises component vitamin B compound 5 ~ 15mg/mL, the component of vitamin C 5 ~ 15mg/mL and following volumn concentration: egg yolk 10% ~ 20%, milk 10% ~ 20%, honey 2% ~ 5%, fly maggot homogenate 10% ~ 20%, flour mite homogenate 10% ~ 20% and preservative 0.5% ~ 2%, surplus is water.
More preferably, described liquid feed comprises component vitamin B compound 5 ~ 10mg/mL, the component of vitamin C 5 ~ 10mg/mL and following volumn concentration: egg yolk 10% ~ 15%, milk 10% ~ 15%, honey 4% ~ 5%, fly maggot homogenate 10% ~ 15%, flour mite homogenate 10% ~ 15% and preservative 0.5% ~ 1%, surplus is water.
Preferably, described milk is the fresh milk of commercially available plain chocolate product, commercially available fresh milk product or the firm output of milk cow through sterilizing.
Described fly maggot homogenate is that fly maggot larva is added water, carries out homogenate, and homogenate is centrifugal, gets supernatant and namely obtains fly maggot homogenate; Described flour mite homogenate gets flour mite to add water, carries out homogenate, and homogenate is centrifugal, gets supernatant and namely obtain flour mite homogenate.
Preferably, described preservative is the combination of one or more in potassium sorbate, Sodium Benzoate, streptococcus lactis, natamycin, dehydro sodium acetate, propylparaben or dehydroacetic acid; When combinationally using, mixed proportion does not do considered critical.
More preferably, described preservative is the combination of one or more in potassium sorbate, Sodium Benzoate or propylparaben; When combinationally using, mixed proportion does not do considered critical.
Preferably, the compound method of described liquid feed is as follows:
S1. fly maggot larva is added water, carry out homogenate, homogenate is centrifugal, gets supernatant for subsequent use;
S2. get flour mite and add water, carry out homogenate, homogenate is centrifugal, gets supernatant for subsequent use;
S3. egg yolk, milk and honey is taken in proportion, for subsequent use;
S4. vitamin B compound and vitamin C are pulverized respectively, for subsequent use;
S5. by ready for S1 ~ S4 each component, mix, add preservative, surplus water polishing, stirs, and both obtains liquid feed.
Preferred as one, in the component of liquid feed of the present invention and the water preparing described liquid feed used be sterile water; Aseptic condition is more conducive to the long-term preservation of liquid feed.
The present invention has following beneficial effect:
The invention provides a kind of rearing method of Phytoseiidae Predatory Mites, the ecological conditions such as liquid feed, temperature and humidity required in artificial feeding process are through that large quantity research draws, adopt aforesaid liquid feed and raising method, successfully can breed Predatory Mites, and feeding effect is very good.The Predatory Mites of raising is used for biological control, can significantly improve output and the quality of crop, reduces residue of pesticide, is conducive to preserving the ecological environment.
In addition, the form that liquid feed is made folliculus by the present invention is used for raising Predatory Mites, during Predatory Mites feed, the surface that initiatively can be attached to folliculus takes food, what add Predatory Mites in unit volume takes food surface area, can not only space be saved, effectively can also prevent the volatilization of liquid feed, reduce costs further.
The rearing method technique of Phytoseiidae Predatory Mites of the present invention is simple, and cost is low, require low, and feeding environment is cleaner, is highly suitable for the large-scale promotion application of industrialization to feeding environment.
Embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment does not limit in any form to the present invention.Unless stated otherwise, the present invention adopts reagent, method and apparatus are the art conventional reagent, method and apparatus.
embodiment 1
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 10ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 10ml supernatant for subsequent use;
S3. egg yolk 15ml is got for subsequent use;
S4. milk 15ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 1ml potassium sorbate, surplus, stirs to 100ml with sterile water polishing.
2, the lapping (described lapping is stretchable sealed membrane or stretchable preservative film, and following embodiment is same) elongated by liquid feed makes folliculus, and the specification of folliculus is roughly 1 ~ 2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access Amblyseius barkeri (
amblyseiusbarkeri) carry out cultivation and breed.Cultivating the condition of breeding is temperature 25 DEG C, relative moisture 70%.
Result shows, and raise about 10 days, Amblyseius barkeri can expand rapidly numerous, and the amount of Amblyseius barkeri approximately increases by 2 ~ 10 times weekly.
embodiment 2
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 13ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 10ml sterile water, then that homogenate is centrifugal, get 13ml supernatant for subsequent use;
S3. egg yolk 12ml is got for subsequent use;
S4. milk 12ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 0.8g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 0.8ml Sodium Benzoate, surplus, stirs to 100ml with sterile water polishing.
2, the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) that liquid feed elongates is made folliculus, the specification of folliculus is roughly 1 ~ 2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access amblyseius cucumeris oudeman (
neoseiuluscucumeris) carry out cultivation and breed.Cultivating the condition of breeding is temperature 26 DEG C, relative moisture 80%.
Result shows, and raise about 10 days, amblyseius cucumeris oudeman can expand rapidly numerous, and the amount of amblyseius cucumeris oudeman approximately increases by 2 ~ 10 times weekly.
embodiment 3
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 11ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 10ml sterile water, then that homogenate is centrifugal, get 11ml supernatant for subsequent use;
S3. egg yolk 14ml is got for subsequent use;
S4. milk 14ml is got for subsequent use;
S5. honey 4ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 0.5g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 1ml propylparaben, surplus, stirs to 100ml with sterile water polishing.
2, the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) that liquid feed elongates is made folliculus, the specification of folliculus is roughly 1 ~ 2cm(but does not therefore limit the present invention).
3, be placed in culture apparatus by folliculus, access Amblyseius barkeri carries out cultivation and breeds.Cultivating the condition of breeding is temperature 27 DEG C, relative moisture 70%.
Result shows, and raise about 10 days, Amblyseius barkeri can expand rapidly numerous, and the amount of Amblyseius barkeri approximately increases by 2 ~ 10 times weekly.
embodiment 4
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 12ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 13ml sterile water, then that homogenate is centrifugal, get 15ml supernatant for subsequent use;
S3. egg yolk 15ml is got for subsequent use;
S4. milk 15ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1.5g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 2ml Sodium Benzoate, surplus, stirs to 100ml with sterile water polishing.
2, the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) that liquid feed elongates is made folliculus, the specification of folliculus is roughly 1 ~ 2cm(but does not therefore limit the present invention).
3, be placed in culture apparatus by folliculus, access amblyseius cucumeris oudeman carries out cultivation and breeds.Cultivating the condition of breeding is temperature 28 DEG C, relative moisture 70%.
Result shows, and raise about 10 days, amblyseius cucumeris oudeman can expand rapidly numerous, and the amount of amblyseius cucumeris oudeman approximately increases by 2 ~ 10 times weekly.
embodiment 5
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 13ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 13ml supernatant for subsequent use;
S3. egg yolk 10ml is got for subsequent use;
S4. milk 10ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1.5g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 2.5ml propylparaben, surplus, stirs to 100ml with sterile water polishing.
2, the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) that liquid feed elongates is made folliculus, the specification of folliculus is roughly 1 ~ 2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access Rui Shi genus amblyseius berlese (
neoseiulusswirskii) carry out cultivation and breed.Cultivating the condition of breeding is temperature 24 DEG C, relative moisture 70%.
Result shows, and raise about 10 days, Rui Shi genus amblyseius berlese can expand rapidly numerous, and the amount of Rui Shi genus amblyseius berlese approximately increases by 2 ~ 10 times weekly.
embodiment 6
1, obtaining liq feed
The compound method of liquid feed is as follows:
S1. 20 fly maggot larvas are added the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 10ml supernatant for subsequent use;
S2. get 5g flour mite and add the homogenate in electric homogenate machine of 15ml sterile water, then that homogenate is centrifugal, get 15ml supernatant for subsequent use;
S3. egg yolk 11ml is got for subsequent use;
S4. milk 11ml is got for subsequent use;
S5. honey 5ml is got for subsequent use;
S6., after vitamin B compound and vitamin C being pulverized respectively, according to dosage respectively get 1g and be placed in container;
S7. be mixed in the container of S6 by ready for S1 ~ S6 each component, add 1ml dehydro sodium acetate, surplus, stirs to 100ml with sterile water polishing.
2, the lapping (described lapping is stretchable sealed membrane or stretchable preservative film) that liquid feed elongates is made folliculus, the specification of folliculus is roughly 1 ~ 2cm(but does not therefore limit the present invention).
3, folliculus is placed in culture apparatus, access Si Shi genus amblyseius berlese (
amblyseiusswirskii) carry out cultivation and breed.Cultivating the condition of breeding is temperature 25 DEG C, relative moisture 60%.
Result shows, and raise about 10 days, Si Shi genus amblyseius berlese can expand rapidly numerous, and the amount of Si Shi genus amblyseius berlese approximately increases by 2 ~ 10 times weekly.
Claims (3)
1. the rearing method of a Phytoseiidae Predatory Mites, it is characterized in that, the technological process of this rearing method comprises: obtaining liq feed, with lapping, liquid feed is encapsulated, make folliculus, folliculus is placed in the culture apparatus of 75 ~ 90 DEG C of sterilizings, in culture apparatus, accesses Predatory Mites, cultivate;
Wherein, described lapping is stretchable sealed membrane or stretchable preservative film;
Described liquid feed comprises following component: vitamin B compound 5 ~ 20mg/mL, the component of vitamin C 5 ~ 20mg/mL and following volumn concentration: egg yolk 10% ~ 30%, milk 10% ~ 30%, honey 2% ~ 10%, fly maggot homogenate 10% ~ 50%, flour mite homogenate 10% ~ 50% and preservative 0.5% ~ 3%, surplus is water;
Described fly maggot homogenate is that fly maggot larva is added water, carries out homogenate, and homogenate is centrifugal, gets supernatant and namely obtains fly maggot homogenate; Described flour mite homogenate gets flour mite to add water, carries out homogenate, and homogenate is centrifugal, gets supernatant and namely obtain flour mite homogenate;
Described preservative is the combination of one or more in potassium sorbate, Sodium Benzoate, streptococcus lactis, natamycin, dehydro sodium acetate, propylparaben or dehydroacetic acid;
Described Phytoseiidae Predatory Mites is amblyseius cucumeris oudeman, Amblyseius barkeri, Si Shi genus amblyseius berlese or Rui Shi genus amblyseius berlese.
2. the rearing method of Phytoseiidae Predatory Mites according to claim 1, is characterized in that, the condition of described cultivation is temperature 24 ~ 28 DEG C, relative moisture 60 ~ 90%.
3. the rearing method of Phytoseiidae Predatory Mites according to claim 1, is characterized in that, the condition of described cultivation is temperature 25 DEG C, relative moisture 70%.
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| CN104222661A (en) * | 2014-09-26 | 2014-12-24 | 张可池 | Nutrition-supplementing additive for predatory mite |
| CN105746254A (en) * | 2016-03-14 | 2016-07-13 | 福建艳璇生物防治技术有限公司 | Method for preventing red spiders, thrips and aleyrodids on strawberries by amblyseius cucumeris |
| CN107432388B (en) * | 2017-07-24 | 2020-08-14 | 广西南亚热带农业科学研究所 | Cassava nutrient solution for efficiently propagating tetranychus cinnabarinus |
| CN107361027A (en) * | 2017-08-31 | 2017-11-21 | 中国农业科学院植物保护研究所 | A kind of device and method using man-made feeds circulation sterilization raising predatism stinkbug |
| CN107927356B (en) * | 2017-12-06 | 2021-07-20 | 中国农业科学院植物保护研究所 | Colloidal artificial improved feed for indoor propagation of amblyseius cucumeris population |
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|---|---|---|---|---|
| CN101049098A (en) * | 2007-03-15 | 2007-10-10 | 福建农林大学 | Culture medium for preserving species of mites |
| CN101049097A (en) * | 2007-03-15 | 2007-10-10 | 福建农林大学 | Conservation method for parent species of predatory mites |
| CN101268772B (en) * | 2008-05-23 | 2012-03-21 | 北京依科曼生物技术有限公司 | Predatory mites producing method |
| CN101406167A (en) * | 2008-09-25 | 2009-04-15 | 福建农林大学 | Method for considerably feeding and using ascidae |
| WO2010106138A1 (en) * | 2009-03-20 | 2010-09-23 | Novartis Ag | Pesticide composition |
| CN101790972B (en) * | 2010-03-15 | 2011-11-16 | 于丽辰 | Method for feeding Tarsonemus confusus |
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2013
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