CN101790972B - Method for feeding Tarsonemus confusus - Google Patents
Method for feeding Tarsonemus confusus Download PDFInfo
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- CN101790972B CN101790972B CN2010101337090A CN201010133709A CN101790972B CN 101790972 B CN101790972 B CN 101790972B CN 2010101337090 A CN2010101337090 A CN 2010101337090A CN 201010133709 A CN201010133709 A CN 201010133709A CN 101790972 B CN101790972 B CN 101790972B
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Abstract
The invention relates to a method for feeding Tarsonemus confuses. In the method, plant leaves or a culture medium containing potato powder, sucrose and agar is taken as a feed for the Tarsonemus confusus; the Tarsonemus confusus is put into the feed; and the production of the Tarsonemus confusus is carried out under the condition that the temperature is between 20 and 30 DEG C and the RH is 80 to 95 percent. The method for feeding the Tarsonemus confusus has the advantages of low cost, readily available raw materials, and large-scale factory production of the Tarsonemus confusus so as to meet the requirement on scientific research, and can be applied as a feed simultaneously.
Description
Technical field
The present invention relates to a kind of raising method of acarid, specifically, relate to the raising method of a kind of random tarsonemid.
Background technology
Recent study finds that diplostomiasis such as peach, apple are to endanger institute extremely by random tarsonemid, and apple reaches 50%-90% by this mite harm incidence of disease, has had a strong impact on fruit matter and quality.For biological property, the harm feature of grasping this mite, thereby it is increasing to the research work demand of this evil mite to carry out favourable control.Yet, become the major obstacle of this worm research now, so a large amount of propagation techniques of the random tarsonemid of active demand because the restriction of season or region can not obtain experiment at any time and use worm in the past.
Summary of the invention
The objective of the invention is to solve above-mentioned random tarsonemid experiment worm problem, the raising method of the random tarsonemid of a large amount of breedings of exploitation is to satisfy the needs of research work, simultaneously as feed applications.
In order to realize the object of the invention, the invention provides the raising method of a kind of random tarsonemid, it is with plant leaf blade or contains the feed of the medium of dehydrated potato powder, sucrose and agar as random tarsonemid, in feed, insert random tarsonemid, then at temperature 20-30 ℃, under the condition of RH80-95%, cultivated 28～32 days, carry out the production of random tarsonemid.
Aforesaid raising method, wherein said plant leaf blade comprise one or more in fresh or withered apple, Chinese chestnut, the rose of Sharon, lily magnolia, grape, Amur honeysuckle, Chinese rose, oak-leaved goosefeet, peach, large cherry, China pink, grass family blade of grass, the cloves blade.
Aforesaid raising method, the preparation method of wherein said plant leaf blade feed may further comprise the steps: the 1) collection of plant leaf blade: one or more in collection apple, Chinese chestnut, the rose of Sharon, lily magnolia, grape, Amur honeysuckle, Chinese rose, oak-leaved goosefeet, peach, large cherry, China pink, grass family blade of grass, the cloves blade; 2) cleaning of plant leaf blade: with 1) the blade water of collecting cleans, and removes dust and addendum; 3) oven dry of plant leaf blade: with 2) blade of Xi Jinging places in the baking oven, and temperature is provided with 40-60 ℃, the 6-10h oven dry; 4) sterilization of plant leaf blade: with 3) Hong Gan blade is in 121 ℃, and it is interior standby that sterilization 15-20min, the blade after the sterilization are kept at the hermetically drying device; 5) plant leaf blade moistening and insert random tarsonemid: with 4) plant leaf blade that is stored in the hermetically drying device is pulverized, and places in the open-top receptacle, is positioned over 20-30 ℃, 16-18h in the environment of RH80-95%, promptly.
Aforesaid raising method, preferably starch or the dusty yeast of adding 10wt% in the blade that step 5) is pulverized.
Aforesaid raising method inserts random tarsonemid in described plant leaf blade feed, every gram blade inserts the random tarsonemid of 100-200 head.
Aforesaid raising method, the preparation method of wherein said plant leaf blade feed can also adopt following method, and it may further comprise the steps: the 1) collection of plant leaf blade: one or more in collection fresh apple, peach, the large cherry blade; 2) cleaning of plant leaf blade: with 1) blade of Shou Jiing cleans with running water, removes dust and addendum; 3) drying of plant leaf surface: inhale defoliation face moisture content with blotting paper, place in the open-top receptacle, promptly.
When utilizing in fresh apple, peach, the large cherry blade one or more as feed, aforesaid raising method inserts random tarsonemid in described plant leaf blade feed, and every square decimeter of blade inserts the random tarsonemid of 80-150 head.
Aforesaid raising method, the wherein said preparation method who contains the medium feed of dehydrated potato powder, sucrose and agar may further comprise the steps: 1) culture medium preparation: take by weighing raw material dehydrated potato powder, sucrose and agar in proportion, be dissolved in water, make that dehydrated potato powder concentration is 180-200g/L, sucrose concentration is 18-20g/L, and agar concentration is 18-20g/L; 2) the medium sterilization of medium: with 1) places autoclave interior in 121 ℃, sterilization 15-20min, and culture medium after sterilization is kept in the refrigerator standby; 3) processing of medium: with the dilution nitrile bacterium azoles missible oil be sprayed onto media surface equably, treat the media surface moisture drying after, promptly.
Aforesaid raising method, wherein nitrile bacterium azoles missible oil dilute with water is 2000 times.Nitrile bacterium azoles missible oil is commercial product, and concentration is generally 12-12.5%.
When utilizing above-mentioned medium as feed, aforesaid raising method inserts random tarsonemid in the described medium feed that contains dehydrated potato powder, sucrose and agar, and every square decimeter of medium inserts the random tarsonemid of 80-150 head.
By technique scheme, the present invention has following advantage and beneficial effect at least:
(1) the present invention is used to raise the raw material of the feed of random tarsonemid from plant leaf blade or easy purchase, and cost is low.
(2) raising method of the random tarsonemid of the present invention can carry out the large-scale industrialized production of random tarsonemid, to satisfy the needs of research work, simultaneously as feed applications.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
The technological process of carrying out the batch production production of the random tarsonemid of the present invention may further comprise the steps in order:
1) plant leaf blade is collected: summer, autumn gather or the collection of winter in spring comprises blades such as apple, Chinese chestnut, the rose of Sharon, lily magnolia, grape, Amur honeysuckle, Chinese rose, oak-leaved goosefeet, peach, large cherry, China pink, grass family blade of grass and cloves;
2) plant leaf blade cleans: with 1) blade collected cleans with running water, removes dust and addendum gets final product;
3) plant leaf blade oven dry: with 2) blade of cleaning places in the baking oven, and temperature is provided with 50 ℃, and 8h can be dried;
4) plant leaf blade sterilization: with 3) blade of oven dry is wrapped up in bag with 10 layers of newspaper and is placed on sterilization in the autoclave sterilization pot, and 121 ℃, 20min, the blade after the sterilization are kept in the hermetically drying device standby;
5) plant leaf blade is moistening and insert random tarsonemid: with 4) plant leaf blade that is stored in the hermetically drying device pulverizes, the starch that in the blade of pulverizing, adds 10wt% afterwards, place that diameter 9cm's remove to cover culture dish, place 25 ℃, in the environment of RH93%, behind the 16h (this moment leaf water content be about 25%-35%) can insert random tarsonemid, every gram blade inserts about 150 random tarsonemids, 26 ℃, can reach every gram blade in 30 days and contain about 4500 random tarsonemids, also can determine to insert the quantity of random tarsonemid according to actual needs and raising temperature is set;
6) cultivation of random tarsonemid: with 5) plant leaf blade that has been connected to random tarsonemid places 25 ℃ together with culture dish, cultivates breeding 30 days in the environment of RH93%, can obtain a large amount of random tarsonemids.
Embodiment 2
1) collection of plant leaf blade: summer, autumn gather or the collection of winter in spring comprises blades such as apple, Chinese chestnut, the rose of Sharon, lily magnolia, grape, Amur honeysuckle, Chinese rose, oak-leaved goosefeet, peach, large cherry, China pink, grass family blade of grass and cloves;
2) cleaning of plant leaf blade: with 1) blade of Shou Jiing cleans with running water, removes dust and addendum gets final product;
3) oven dry of plant leaf blade: with 2) blade of Xi Jinging places in the baking oven, and temperature is provided with 50 ℃, and 8h can be dried;
4) sterilization of plant leaf blade: with 3) Hong Gan blade is wrapped up in bag with 10 layers of newspaper and is placed on sterilization in the autoclave sterilization pot, and 121 ℃, 15min, the blade after the sterilization are kept in the hermetically drying device standby;
5) plant leaf blade moistening and insert random tarsonemid: with 4) plant leaf blade that is stored in the hermetically drying device is pulverized, the dusty yeast that in the blade of pulverizing, adds 10wt% afterwards, place that diameter 9cm's remove to cover culture dish, place 25 ℃, in the environment of RH93%, behind the 16h (this moment leaf water content be about 25%-35%) can insert random tarsonemid, every gram blade inserts about 200 random tarsonemids, 26 ℃, can reach every gram blade in 30 days and contain about 5000 random tarsonemids, also can determine to insert the quantity of random tarsonemid according to actual needs and raising temperature is set;
6) cultivation of random tarsonemid: with 5) plant leaf blade that has been connected to random tarsonemid places 25 ℃ together with culture dish, cultivates breeding 30 days in the environment of RH93%, can obtain a large amount of random tarsonemids.
Embodiment 3
1) collection of plant leaf blade: gather in land and the greenhouse plant leaf blades such as fresh apple, peach, large cherry one or more;
2) plant leaf blade cleaning treatment: with 1) blade of Shou Jiing cleans with running water, removes dust and addendum;
3) drying on plant leaf blade surface: the blade of cleaning is inhaled defoliation face moisture content with blotting paper;
4) insert random tarsonemid: with 3) blade place diameter 9cm remove to cover culture dish, place 25 ℃, in the environment of RH93%, insert random tarsonemid behind the 16h, every square decimeter of blade inserts the random tarsonemid of 80-150 head.
5) cultivation of random tarsonemid: with 4) plant leaf blade that has been connected to random tarsonemid places 25 ℃ together with container, cultivates breeding 30 days in the environment of RH93%, can obtain a large amount of random tarsonemids.
Embodiment 4
1) preparation of medium: use dehydrated potato powder 200g, sucrose 20g, agar 20g, water 1000ml, uncomfortable pH value is made medium;
2) medium of making is placed in the autoclave in 121 ℃, sterilization 20min, culture medium after sterilization is kept in the refrigerator standby;
3), handle medium with the nitrile bacterium azoles missible oil after the dilution with 2000 times of the nitrile bacterium azoles missible oil dilute with waters of 12.5wt%;
4) insert random tarsonemid: treat 3) the media surface moisture drying after insert random tarsonemid, every ware (the culture dish specification is 90*15mm) inserts 60 random tarsonemids;
5) culture dish that the is connected to random tarsonemid cultivation of random tarsonemid: with 4) places 25 ℃, cultivates breeding 30 days in the environment of RH93%, can obtain a large amount of random tarsonemids.
Though above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (6)
1. the raising method of a random tarsonemid, it is characterized in that plant leaf blade or contain the feed of the medium of dehydrated potato powder, sucrose and agar as random tarsonemid, in feed, insert random tarsonemid, then at temperature 20-30 ℃, under the condition of RH80-95%, cultivated 28～32 days, and carried out the production of random tarsonemid; Wherein said plant leaf blade comprises one or more in fresh or withered apple, Chinese chestnut, the rose of Sharon, lily magnolia, grape, Amur honeysuckle, Chinese rose, oak-leaved goosefeet, peach, large cherry, China pink, grass family blade of grass and the cloves blade; The preparation method of described plant leaf blade feed may further comprise the steps:
1) collection of plant leaf blade: one or more in collection apple, Chinese chestnut, the rose of Sharon, lily magnolia, grape, Amur honeysuckle, Chinese rose, oak-leaved goosefeet, peach, large cherry, China pink, grass family blade of grass and the cloves blade;
2) cleaning of plant leaf blade: with 1) the blade water of collecting cleans, and removes dust and addendum;
3) oven dry of plant leaf blade: with 2) blade of Xi Jinging places in the baking oven, and temperature is provided with 40-60 ℃, the 6-10h oven dry;
4) sterilization of plant leaf blade: with 3) Hong Gan blade is in 121 ℃, and it is interior standby that sterilization 15-20min, the blade after the sterilization are kept at the hermetically drying device;
5) plant leaf blade moistening and insert random tarsonemid: with 4) plant leaf blade that is stored in the hermetically drying device is pulverized, and places in the open-top receptacle, is positioned over 20-30 ℃, 16-18h in the environment of RH80-95%, promptly.
2. raising method according to claim 1 is characterized in that adding starch or the dusty yeast of 10wt% in the blade that step 5) is pulverized.
3. raising method according to claim 1 and 2 is characterized in that inserting random tarsonemid in described plant leaf blade feed, every gram blade inserts the random tarsonemid of 100-200 head.
4. raising method according to claim 1 is characterized in that the wherein said preparation method who contains the medium feed of dehydrated potato powder, sucrose and agar may further comprise the steps:
1) culture medium preparation: take by weighing raw material dehydrated potato powder, sucrose and agar in proportion, be dissolved in water, make that dehydrated potato powder concentration is 180-200g/L, sucrose concentration is 18-20g/L, and agar concentration is 18-20g/L;
2) the medium sterilization of medium: with 1) places autoclave interior in 121 ℃, sterilization 15-20min, and culture medium after sterilization is kept in the refrigerator standby;
3) processing of medium: with the dilution nitrile bacterium azoles missible oil be sprayed onto media surface equably, treat the media surface moisture drying after, promptly.
5. raising method according to claim 4 is characterized in that wherein 2000 times of nitrile bacterium azoles missible oil dilute with waters.
6. according to claim 4 or 5 described raising method, it is characterized in that in the described medium feed that contains dehydrated potato powder, sucrose and agar, inserting random tarsonemid, every square decimeter of medium inserts the random tarsonemid of 80-150 head.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101337090A CN101790972B (en) | 2010-03-15 | 2010-03-15 | Method for feeding Tarsonemus confusus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101337090A CN101790972B (en) | 2010-03-15 | 2010-03-15 | Method for feeding Tarsonemus confusus |
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| CN101790972A CN101790972A (en) | 2010-08-04 |
| CN101790972B true CN101790972B (en) | 2011-11-16 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103734089B (en) * | 2013-12-26 | 2016-04-06 | 中山大学 | A kind of rearing method of Phytoseiidae Predatory Mites |
| CN107494625A (en) * | 2017-09-26 | 2017-12-22 | 江苏菇本堂生物科技股份有限公司 | A kind of acarid for planting edible mushroom detects liquid |
| CN108633842A (en) * | 2018-04-10 | 2018-10-12 | 首伯农(北京)生物技术有限公司 | A method of raising tarsonemid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1440646A (en) * | 2002-02-24 | 2003-09-10 | 张艳璇 | Artificial raising method of cuculber amblyseius predatory mite |
| CN101049098A (en) * | 2007-03-15 | 2007-10-10 | 福建农林大学 | Culture medium for preserving species of mites |
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2010
- 2010-03-15 CN CN2010101337090A patent/CN101790972B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1440646A (en) * | 2002-02-24 | 2003-09-10 | 张艳璇 | Artificial raising method of cuculber amblyseius predatory mite |
| CN101049098A (en) * | 2007-03-15 | 2007-10-10 | 福建农林大学 | Culture medium for preserving species of mites |
Non-Patent Citations (1)
| Title |
|---|
| 郅军锐等.侧多食跗线螨的生物学和生态学特性.《山地农业生物学报》.2001,第20卷(第2期),106-109. * |
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