CN110257074A - A kind of preparation of wood vinegar bacillus subtilis bacterium solution soil conditioner and its application method - Google Patents
A kind of preparation of wood vinegar bacillus subtilis bacterium solution soil conditioner and its application method Download PDFInfo
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- CN110257074A CN110257074A CN201910257315.7A CN201910257315A CN110257074A CN 110257074 A CN110257074 A CN 110257074A CN 201910257315 A CN201910257315 A CN 201910257315A CN 110257074 A CN110257074 A CN 110257074A
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- bacillus subtilis
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- 239000002023 wood Substances 0.000 title claims abstract description 95
- 239000000052 vinegar Substances 0.000 title claims abstract description 94
- 235000021419 vinegar Nutrition 0.000 title claims abstract description 94
- 244000063299 Bacillus subtilis Species 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- 239000003516 soil conditioner Substances 0.000 title claims abstract description 24
- 239000002689 soil Substances 0.000 claims abstract description 88
- 239000007788 liquid Substances 0.000 claims abstract description 66
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 21
- 238000004821 distillation Methods 0.000 claims abstract description 14
- 238000001994 activation Methods 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 10
- 238000004519 manufacturing process Methods 0.000 claims abstract description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 8
- 239000007640 basal medium Substances 0.000 claims abstract description 7
- 238000010521 absorption reaction Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 58
- 238000010790 dilution Methods 0.000 claims description 21
- 239000012895 dilution Substances 0.000 claims description 21
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 19
- 150000003839 salts Chemical class 0.000 claims description 17
- 230000004048 modification Effects 0.000 claims description 14
- 238000012986 modification Methods 0.000 claims description 14
- 241000894006 Bacteria Species 0.000 claims description 12
- 230000004913 activation Effects 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 8
- 238000003763 carbonization Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 238000000197 pyrolysis Methods 0.000 claims description 7
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- 238000005259 measurement Methods 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 229960000583 acetic acid Drugs 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 3
- 239000000498 cooling water Substances 0.000 claims description 3
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- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
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- 239000011591 potassium Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 230000005855 radiation Effects 0.000 claims description 2
- 239000004016 soil organic matter Substances 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 17
- 230000001976 improved effect Effects 0.000 abstract description 12
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- 230000010355 oscillation Effects 0.000 description 3
- 230000000630 rising effect Effects 0.000 description 3
- 238000010792 warming Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 238000009833 condensation Methods 0.000 description 2
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- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
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- 230000005764 inhibitory process Effects 0.000 description 2
- 239000003621 irrigation water Substances 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 159000000003 magnesium salts Chemical class 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
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- 238000001179 sorption measurement Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000011269 tar Substances 0.000 description 2
- 239000011276 wood tar Substances 0.000 description 2
- MPDGHEJMBKOTSU-YKLVYJNSSA-N 18beta-glycyrrhetic acid Chemical compound C([C@H]1C2=CC(=O)[C@H]34)[C@@](C)(C(O)=O)CC[C@]1(C)CC[C@@]2(C)[C@]4(C)CC[C@@H]1[C@]3(C)CC[C@H](O)C1(C)C MPDGHEJMBKOTSU-YKLVYJNSSA-N 0.000 description 1
- 241001037822 Bacillus bacterium Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009305 arable farming Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
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- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000012958 reprocessing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 238000004856 soil analysis Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000011289 tar acid Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
- G01N33/245—Earth materials for agricultural purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Soil Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Environmental Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Geology (AREA)
- Remote Sensing (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Materials Engineering (AREA)
- Mechanical Engineering (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
A kind of preparation of wood vinegar bacillus subtilis bacterium solution soil conditioner and its application method, it is related to a kind of preparation of soil conditioner and its application method, the present invention is by configuring LB basal medium, preparation bacillus subtilis bacterium solution, preparing wood vinegar bacillus subtilis bacterium solution, and the activation process of bacillus subtilis, change P in soil H value, soil is improved, plant growth is promoted, improves crop growth character, improving quality；Manufacturing process is mentioned using wood vinegar essence, through decompression, distillation, absorption, improves the purity of wood vinegar, reaches acetum pyrolignosum rectificatum liquid PH≤2.5.Profitable strain and pH value of this product rich in plant growth adjust element, have certain inhibiting effect to the microorganism in soil, sterilization improving effect can be played to soil layer.It is obvious to apply microbial bacterial agent effect of increasing production.In the serious area of perniciousness harm, a kind of new and improved dose as natural green of microbial bacterial agent is degradable, is easy to be received by the soil body, has very big development potentiality.
Description
Technical field
The present invention relates to a kind of preparation of soil conditioner and its application methods, more particularly to a kind of wood vinegar withered grass gemma
The preparation of bacillus liquid soil conditioner and its application method.
Background technique
In modern planting technology, soil function is improved this means that improving the yield of crops, increases economic benefit.But
In crop planting management process, since soil issues, such as salt-soda soil, harmful bacteria cause plant undergrowth, pest and disease damage, subtract
Produce and so on, reduce the economic benefit of grower.
Wood vinegar is the phenol and organic acid solution based on acetic acid, it is that wood generates during charcoal is made
Gas condenses the liquid as obtained from distilling or stand again.Main component is by ketone, alcohol, vitamin, tar and minerals etc.
Various trace elements are composed.Wood vinegar is widely used in agricultural and forestry production, mainly be can be applied to promote plant growth, be prevented
Worm is antibacterial and preserving fruit and vegetable utilizing etc..The adjustable P in soil H value of wood vinegar increases beneficial microbe and improves Soil ventilation
Property, the problems such as efficiently solving the salinization of soil；Wood vinegar has inhibiting effect to the maize ear rots bacterium such as penicillin and Escherichia coli；The wooden vinegar
The phenols contained in liquid has good antioxidant activity, can be used as antistaling agent to reduce the rotting mechanism of fruit and percentage of water loss,
To reach preservation.Wood vinegar is a kind of natural materials, and processing method is simple and easy, and function and effect are significant and from a wealth of sources
It is free from environmental pollution, it can be energy saving to avoid unnecessary waste, it can be seen that, the application prospect of wood vinegar is very wide,
Major contribution certainly will be made to the agricultural development in China.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation of wood vinegar bacillus subtilis bacterium solution soil conditioner and its application sides
The preparation process of method, wood vinegar of the present invention improves the purity of wood vinegar, while effectively reducing objectionable impurities；With wood vinegar and
Bacillus subtilis bacterium solution soil conditioner as main component can effectively change P in soil H value, improve soil, inhibit germ, promote
Plant growth improves crop growth character, improving quality to improve Economic Benefits of Crops.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of wood vinegar bacillus subtilis bacterium solution soil conditioner preparation method, the wood vinegar bacillus subtilis bacterium solution soil change
Good dose of preparation method, it is characterized in that: including following preparation step:
(1) LB basal medium is configured: with HCL solution and NaOH solution the control pH value of 1mol/L between 6.9~7.1,
High pressure sterilization 15 minutes at 121 DEG C is cooled to room temperature；
(2) bacillus subtilis bacterium solution is prepared: by the bacillus subtilis of activation by 2%(mass percent) it is inoculated into above-mentioned LB
In basal medium, bottleneck is shut, 23~25h of isothermal vibration culture in 37 DEG C, 180r/min incubator；
(3) it prepares wood vinegar bacillus subtilis bacterium solution: acetum pyrolignosum rectificatum liquid is added to by 0.05%~0.2% (mass percent)
In above-mentioned bacillus subtilis bacterium solution, 37 DEG C of Yu, 7~9h of isothermal vibration culture in 180r/min incubator；Gained yellowish-brown
Bacterium solution is wood vinegar bacillus subtilis bacterium solution, and viable count is 1.7 × 109~1.8 × 109log10CFU/mL；
Mass percent shared by soil conditioner raw material are as follows: bacillus subtilis bacterium solution 99.8% -99.95%, acetum pyrolignosum rectificatum liquid
0.05%—0.2%；Mixture.
A kind of wood vinegar bacillus subtilis bacterium solution soil conditioner preparation, the activation side of the bacillus subtilis
Method: implementing vessel and LB liquid medium for all glass and carry out high pressure sterilization, with alcohol wipe aseptic operating platform and with ultraviolet
The sterilizing of line light irradiation；And it operates as follows:
(1) it activates the first generation: 100 ml LB liquid mediums is poured into respectively in four test tubes, it is withered that each test tube is inoculated with a needle
Careless bacillus carries out isothermal vibration (37 DEG C of 180r/min) culture for 24 hours；
(2) activate the second generation: 100 ml LB liquid mediums of production are poured into respectively in four test tubes, in every absorption generation
500 μ L bacterium solutions carry out isothermal vibration (37 DEG C of 180r/min) culture for 24 hours；
(3) second generation amplification cultivation also can be regarded as the activation third generation: 300 ml LB liquid mediums of production draw second generation bacterium solution
6mL carries out isothermal vibration culture (180r/min37 DEG C)；The first generation of activation, the second generation are put into -80 DEG C of refrigerators save it is standby
With；The third generation is implemented as activated bacillus subtilis.
A kind of described wood vinegar bacillus subtilis bacterium solution soil conditioner and its preparation, the preparation of the acetum pyrolignosum rectificatum liquid
Method are as follows:
A. wooden stick is placed in closed anoxybiotic drying in drying kettle, and the wooden stick prepared is put into carbonization kettle, dry through four stages
It evaporates pyrolysis, the cooling gas-liquid separation of three-level, distill obtained crude pyroligneous acid；The liquid that gas-liquid separation is gone out, i.e. oil liquid (burnt oil and vinegar
Acid) it is transported in distillation reactor, it is crude pyroligneous acid after distillation；
B. the crude pyroligneous acid is conveyed into distiller, gaseous material is conveyed into rectifier, and rectifier will be after above-mentioned rectifying
Gaseous material is conveyed into condenser and becomes liquid, as acetum pyrolignosum rectificatum liquid.
A kind of wood vinegar bacillus subtilis bacterium solution soil conditioner application method, the method are applied to spray in soil
It applies or fills and apply；150-160 kilograms of modifying agent is preferably applied per acre.
A kind of wood vinegar bacillus subtilis bacterium solution soil conditioner application method, the application method, including toothed oak
The preparation of the wooden wood vinegar biological modification agent: drying toothed oak wood sawdust extrusion forming is put into retort and is heated, in condensing unit
It is passed through cooling water, collects wood vinegar, by filtering, distillation, biological treatment；Then wood vinegar biological modification dilution agent is bottled
It is spare.
A kind of wood vinegar bacillus subtilis bacterium solution soil conditioner application method, the application method, application side
Case and parameter apolegamy are as follows: soil ratio 1:5 extracts soil extraction, takes out 30mL solution and is placed in small beaker, is evaporated baking
Dry measure total salt；The solution such as 20mL, 25mL measurement soil ion is taken to calculate SAR, ESP；Soil ratio 1:1 dissolves soil, surveys
Measure pH value；Potassium bichromate-concentrated sulfuric acid oxidation, additional oil bath pan boil, and measure the organic matter variation of soil, measure soil
Physicochemical property；2mm sieve 250g soil was taken, adds 25mL wood vinegar dilution, stirs evenly and be fitted into beaker, was placed on same
Etc. cultivate under external environments, the dilution of addition in three days takes a soil test analysis for one week.
The advantages and effects of the present invention are:
1. wood vinegar prepared by the present invention be by the processing waste of forest tree, waste wood (material) and be pyrolyzed through biomass carbonization
Preparation, there is positive effect in the reprocessing and comprehensive utilization of waste.
2. wood vinegar bacillus subtilis bacterium solution of the invention is by acetum pyrolignosum rectificatum liquid and bacillus subtilis bacterium bacterium solution certain
Under the conditions of cultivate.P in soil H can effectively be changed with wood vinegar and bacillus subtilis bacterium solution soil conditioner as main component
Value improves soil, inhibits germ, promotes plant growth, improves crop growth character, improving quality.
Detailed description of the invention
The influence curve figure that Fig. 1 various concentration wood vinegar grows bacillus subtilis bacterium.
Specific embodiment
The present invention is described in detail for illustrated embodiment with reference to the accompanying drawing.
A kind of growth promotion wood vinegar bacillus subtilis bacterium bacterium solution additive, mass percent shared by the additive raw material
Are as follows: bacillus subtilis bacterium bacterium solution 99.9%, acetum pyrolignosum rectificatum liquid 0.1%；
The preparation method of growth promotion wood vinegar bacillus subtilis bacterium bacterium solution additive, includes the following steps:
(1) it configures LB basal medium: pH value being adjusted to 7 with the HCl solution of 1mol/L and NaOH solution, the high pressure at 121 DEG C
Sterilizing 15min, it is cooled to room temperature；
(2) bacillus subtilis bacterium bacterium solution is prepared: by the bacillus subtilis bacterium of activation by 2%(mass percent) it is inoculated into
It states in LB basal medium, bottleneck is shut, isothermal vibration culture is for 24 hours in 37 DEG C, 180r/min incubator；Bacillus subtilis
Bacterium bacterium is purchased from market, and producer is Yangzhou Haicheng Biotechnology Co., Ltd.
(3) wood vinegar bacillus subtilis bacterium bacterium solution is prepared: by acetum pyrolignosum rectificatum liquid by 0.1%(mass percent) in addition
It states in bacillus subtilis bacterium bacterium solution, cultivates 8h in 37 DEG C, 180r/min concussion and cultivate case；Gained yellowish-brown bacterium solution is wood
Vinegar liquid bacillus bacterium solution, viable count are 1.8*109 ㏒10CFU/mL；
The activation method of above-mentioned bacillus subtilis: implementing vessel for all glass and LB liquid medium carry out high pressure sterilization,
With alcohol wipe aseptic operating platform and with ultraviolet radiator radiation sterilization；(1) first generation is activated: by 100mlLB fluid nutrient medium point
It does not pour into four test tubes, each test tube is inoculated with a needle bacillus subtilis and carries out (180r/min37 DEG C) of constant temperature oscillation culture
24h；(2) activate the second generation: production 100mlLB fluid nutrient medium is poured into respectively in four test tubes, 500 in every absorption generation
μ L bacterium solution carries out (180r/min37 DEG C) of constant temperature oscillation culture for 24 hours；(3) second generation amplification cultivation also can be regarded as the activation third generation:
300mlLB fluid nutrient medium is made, second generation bacterium solution 6mL is drawn and carries out constant temperature oscillation (180r/min37 DEG C)；By the of activation
A generation, the second generation are put into -80 DEG C of refrigerators and save backup: the third generation is implemented as activated bacillus subtilis.
Above-mentioned acetum pyrolignosum rectificatum liquid the preparation method comprises the following steps:
(1) preparation of wooden stick: with fruit tree, toothed oak wood give up branch, sawdust, floor leftover pieces one or more for raw material, be put into drying
In kettle, closed anoxybiotic carries out humidity discharging processing using outer heating；
(2) preparation of crude pyroligneous acid: the wooden stick prepared is put into carbonization kettle, cold through four stage carbonizations, three-level
But, crude pyroligneous acid is made in gas-liquid separation, distillation；Four stage carbonizations method particularly includes: the first stage, pyrolysis temperature was by normal
Warm uniformly 150 DEG C of heating；Second stage pyrolysis temperature is uniformly warming up to 260 DEG C by 150 DEG C；Phase III, pyrolysis temperature was by 260
DEG C or so be uniformly warming up to 370 DEG C；Fourth stage pyrolysis temperature is uniformly warming up to 700 DEG C by 370 DEG C or so；When temperature reaches
Air inlet, anoxycausis are closed at 400 DEG C.Three-level cooling method particularly includes: by gas-liquid mixture caused by carbonization
It is transported to three-level cooling device, level-one cooling makes gas-liquid mixture temperature will drop to 370 DEG C；Second level cooling makes gas-liquid mixture temperature
Degree is down to 200 DEG C；Three-level cooling makes gas-liquid mixture temperature be down to 65 DEG C.Gas-liquid separation method particularly includes: three-level is cooling
The air-liquid of generation is transported to gas-liquid separator, opens the air inlet of pedestal, and gas is made a gift to someone air accumulator, and liquid is burnt oil and vinegar
Liquid realizes gas-liquid separation；Liquid enters oil-liquid separator；Distillation method particularly includes: the liquid that will be isolated in gas-liquid separator
Body, i.e. oil liquid (tar and acetic acid) convey in distillation reactor, isolate wood tar and wood vinegar stoste；Wherein bottom oil in kettle
Shape object is wood tar, and top is that liquid is wood vinegar stoste；The pressure of distillation still is controlled in negative pressure 3 or so；
(3) prepared by acetum pyrolignosum rectificatum liquid: gained crude pyroligneous acid being distilled, rectifying, condensation, filtering, obtains yellowish-brown liquid, for essence
Wood vinegar processed；The crude pyroligneous acid is conveyed into distiller by distillation process, and crude pyroligneous acid is changed into gaseous state by liquid；Distillation process
Gaseous material is conveyed into rectifier；
Gaseous material after above-mentioned rectifying is conveyed people's condenser by condensation process, and gaseous material becomes liquid；
Filter process liquid is filtered through strainer and active carbon；
Acetum pyrolignosum rectificatum liquid PH2.5；Tar and dust (mg/m3) < 9；255(kg/ tons of yield) hydrogen sulfide
(mg/m3) 19 <
It can be sprayed using this product and filling is applied.It is applied together in conjunction with pouring water.150-160 kilograms is applied per acre.
Embodiment 1
Enforcement place: Hebei province；Implement kind: winter wheat；Implement area: 1 mu of batch, repeats 3, contrast groups are same.
Implement purpose: reducing the saline and alkaline harm for crops in salt-soda soil, reduces salt-soda soil pH value.
Implementation principle: the fertility that soil can be improved in wood vinegar microorganism modifying agent promotes the growth and production of plant.Wood
Vinegar liquid biological modification agent is a kind of degradable substance, is pyrolyzed gained, as plant acid by plant residue, in recent years, mainly
Influence and restraining and sterilizing bacteria and promotion Effects on Plant Growth using wood vinegar biological modification agent to edaphon are still
It is also fewer to the Upgrading of soil.In order to improve Cangzhou City salt-soda soil, this implementation uses northern toothed oak carpentery workshop for raw material system
The wooden vinegar microorganism modifying agent is taken, salinization of soil analysis and preliminary exploratory developemnt are carried out with Cangzhou Typical crops wheat cultivation soil
The optium concentration of wood vinegar biological modification agent improvement, provides foundation for the improvement in salt-soda soil.
Implementation process and equipment:
1 materials and methods
1.1 implement material and instrument
Wood vinegar biological modification agent raw material: toothed oak wood sawdust wood shavings are derived from Fushun relief troops town factory floor.
Pedotheque: picking up from the soil of Meng Cun autonomous county Xu Cun, and 0~10cm is topsoil, i.e. h 1；10~
20cm is Gossypium arboreum×G. bickii, i.e. h 2.
Implement instrument: GC/-MS combined instrument is power & light company, the U.S. (Ther-mo Finnigan) production, model
For Trace GC-2000/DSQ；Thunder magnetic PHS-3C, pH acidometer；Analyze electronic balance, upper current chart level device instrument
Table Co., Ltd, model FA1004；HH-S digital display thermostatical oil bath, Jintan City bank head beneficial friend implement instrument plant；Electricity
Hot blast drying box, Medical Equipment Plant, Shanghai Boxun Industrial Co., Ltd., model GZX-9140MBE.
1.2 implementation method
1.2.1 the preparation of toothed oak wood wood vinegar biological modification agent
Drying toothed oak wood sawdust extrusion forming is put into retort and is heated, cooling water is passed through in condensing unit, collects the wooden vinegar
Liquid, by filtering, distillation, biological treatment.Then by 50 times of wood vinegar biological modification dilution agent, 400 times, 750 times, 800
Times；4.83,0.976,8.21 yellowish green of toothed oak wood sawdust, muddiness 1.48,83.7；1000 times, 1200 times are attached separately to palm fibre
It is spare in the small reagent bottle of color.
1.2.2 the preparation of soil raw material
After air-drying using wooden mallet large clod being broken into pieces gently, then cross the sieve of 1mm, 0.15mm, be respectively charged into self-styled
Bag, finishes number, spare.
1.2.3 implementation method
Soil ratio 1:5 extracts soil extraction, takes out 30mL solution and is placed in small beaker, is evaporated drying measurement total salt；
The solution such as 20mL, 25mL measurement soil ion is taken to calculate SAR, ESP；Soil ratio 1:1 dissolves soil, measures pH value；
Potassium bichromate-concentrated sulfuric acid oxidation, additional oil bath pan boil, and measure the organic matter variation of soil, measure the physicochemical property of soil.
2mm sieve 250g soil was taken, 25mL wood vinegar dilution is added, stirs evenly and is fitted into beaker, was placed on same outer
It is cultivated under boundary's environment, the dilution of addition in three days, takes within one week a soil test analysis.
1.2.4 data statistical approach
Software statistics data are analyzed using origin and draw correlogram.
The salinity of wheatland topsoil is mainly gathered in surface, and surface contains residual branch and loses leaf, gives edaphon
Good existence place is provided.
Existing variation tendency is analyzed, SAR, ESP in first week, in 50 times of wood vinegar dilution processing soil
Fall is maximum；There is rise trend in second week, and at 750 times, SAR, ESP value are minimum；Third Zhou Shiyu
First week similar, and SAR, ESP value decline, and 750 times of extension rate is best.Illustrate it is not that concentration is got over
Greatly, most suitable to the sodium adsorption ratio of soil and basicity improvement.PH of 750 times of the wooden vinegar dilution to soil at first week
Value influences maximum, and 50 times of effect is placed in the middle；Second and third comprehensive week, the soil pH value of 750 times of wooden vinegar dilution processing and
Initial value difference is maximum, and improved effect is best.First week phenomenon is completely contradicted with corresponding period improved effect, and first week
When 400 times of wooden vinegar dilution processing soil in salt content it is maximum, 750 times of salt content is minimum；Second week has decline
Trend, 750 times of values are minimum, and 400 times are taken second place, and 50 times of salt content maximums, with time change, 750 times in third
In week, value added is minimum, and 50 times and 400 times increase to identical value.Salt content depends primarily on the composition of soil intermediate ion
Ingredient, topsoil salt content is maximum, the addition of wood vinegar biological modification agent, with soil constituent reacting forming complex but excessively
Salt can replace the salt of soil again, form a kind of new salt, stay in the soil body.Organic matter changing rule is complicated, and first week
When, organic matter decline at only 400 times illustrates that the microorganism in such soil adapts to 400 times of the wooden vinegar dilution
Performance is worst, and the breeding and growth that 50 times, 750 times can temporarily stimulate microorganism, decompose the broken branches and fallen leaves formed it is organic
Matter；In second week, 400 times of organic matter rises, 50 times, 750 times of organic matter decline, it may be possible to due to adapting to 400 times
Microorganism and adapt to 50 times, 750 times of microorganism competitiveness it is strong, it is suppressed that the growth of corresponding microorganism in third week, is owned
Organic matter all decline, 400 times decline 50 times of Amplitude Ratios, 750 times decline amplitudes want it is small and decline end value
It is equal, illustrate to be added for a long time wood vinegar biological modification agent (concentration is relatively large) may cause microorganism cannot be in the soil body
Existence, so that organic matter decline is even also smaller than initial value.In summary regular, it is within one week best improvement time, 750
Extension rate improved effect is best.Certain rule is presented in all variations, and variation tendency is consistent.ESP is with the time
Variation, presentation first reduce the variation tendency for rising decline again afterwards.SAR sodium adsorption ratio is identical as ESP variation tendency, only
What is be worth is in different size.SAR, ESP, which are reduced, illustrates that the content of the calcium and magnesium salt in soil in the soil increases, and then relative reduction
Soluble sodium ion in soil is relatively suitble to plant growth, but as time increases, continues that wood is added toward inside soil
Vinegar dilution is mutually set calcium and magnesium salt and sodium salt due to the something qualitative response in a small amount of substance and the soil body in wood vinegar again
It changes, therefore SAR, ESP are in increase trend again, then in cycles, are risen again after reduction, the process reduced again after rising.
PH pH inside first week is decreased obviously, and fall is maximum；In second week, pH value is gone up, but still smaller than initial value, still
There is apparent improved effect；Third week, 1000 times of the wooden vinegar dilution handle the pH value in soil and decline, 800 times,
1200 times still in rising trend.Illustrate to be best in the improved effect of first week wood vinegar, and 1000 times of dilution times
The pH improved effect of several pairs of soil is best.After 1200 times of wooden vinegar dilution improvement, the soil salt variety rate of soil is maximum
, at first and second week, total salt quantity is gradually reduced, and third week total salt quantity is gone up.In addition wood vinegar for a long time, wood vinegar and soil
Soluble organic fraction redistribute, cause soluble-salt to increase.Organic matter first week, second week it is lower than initial value, and
Third week increases considerably, all higher than initially, and organic matter is minimum at 800 times, concentration highest, to edaphon inhibitory effect
It is most obvious, it is best to 1000 times of adaptation effects, and 1200 times of adaptability is placed in the middle, but 1200 times organic when third week
Matter content is maximum.The organic matter variation of soil is mainly related with microorganism, does not adapt to wood vinegar in the last fortnight microorganism
It is added, and after two weeks, microorganism gradually adapts to environment, makes full use of wood vinegar, and organic matter is caused to rise, and illustrates the last fortnight wood
The growth and respiratory capacity of vinegar liquid inhibition microorganism.In summary it analyzes, 1200 times of extension rate is best, and
Improved effect is best within one week, but the increase in order to guarantee the soil organism simultaneously, it is also necessary to other ameliorative measures is added, in addition water
The desalinization of soil by flooding or leaching effect；The phenomenon that salt branch is gradually reduced, and does not have rise.
It analyzes based on the above results, it is dilute using wood vinegar biological modification agent when improveing the topsoil of emergence rate 60%
Salinity increases considerably when releasing liquid inside first week, this is not identical as expected result, illustrates that wood vinegar improvement saline-alkali soil is
A very complicated process.But on forefathers' Research foundation, the desalinization of soil by flooding or leaching effect of irrigation water can reduce field salt content, due to
Wood vinegar and Zinc fractions complex compound, in conjunction with irrigation water, the two complements each other, and undoubtedly can reduce the salinity on surface, is suitable for
Object growth.The Gossypium arboreum×G. bickii for improveing emergence rate 60%, index value can be lower than the value before improvement in first week, illustrates wood vinegar
Dilution can significantly improve saline-alkali soil.The topsoil and Gossypium arboreum×G. bickii for improveing wheatland emergence rate 60% must use difference
The wood vinegar of concentration, the optium concentration of topsoil improvement is 750 times of dilutions, and the best improvement concentration of Gossypium arboreum×G. bickii
For 1200 times of dilutions, best improvement time is one week.So in practical applications, according to the difference of arable farming depth,
Select suitable wood vinegar dense.
Application wood vinegar biological modification agent uniformly sprays in the soil body, and best spraying time is before transplanting crop
One week.Wheatland application, is irrigated according to scheme per acre, and different times measure the plant height of wheat, blade, photosynthetic rate.Harvest and comparison
The comparison of wheatland yield and quality.According to the changing rule of organic matter, it can learn that addition wood vinegar is equal to the microorganism in soil
There is certain inhibiting effect, has plenty of and be not reflected inside first week.Comprehensive three weeks situations, microorganism is by different degrees of
Inhibition, illustrate the improvement to different soil, apply the wood vinegar of various concentration, bactericidal effect can be played.Various concentration
Wood vinegar is inconsistent to the inhibitory effect of the microorganism of same soil layer, illustrates wood vinegar to the inhibitory effects of different microorganisms not
Together, complicated strain is included in the soil body.Currently, saline-alkali soil uses a variety of modification methods, technology all comparative maturities, especially in salt
Alkali endangers very serious area, a kind of new and improved dose as natural green of wood vinegar, degradable, is easy to be received by the soil body,
Undoubtedly there is very big development potentiality.By implementing to apply upper definite effect in improvement salt-soda soil certainly with data analysis.
Claims (6)
1. a kind of wood vinegar bacillus subtilis bacterium solution soil conditioner preparation method, which is characterized in that the wood vinegar withered grass bud
The preparation method of spore bacillus liquid soil conditioner, it is characterized in that: including following preparation step:
(1) LB basal medium is configured: with HCL solution and NaOH solution the control pH value of 1mol/L between 6.9~7.1,
High pressure sterilization 15 minutes at 121 DEG C is cooled to room temperature；
(2) bacillus subtilis bacterium solution is prepared: by the bacillus subtilis of activation by 2%(mass percent) it is inoculated into above-mentioned LB
In basal medium, bottleneck is shut, 23~25h of isothermal vibration culture in 37 DEG C, 180r/min incubator；
(3) it prepares wood vinegar bacillus subtilis bacterium solution: acetum pyrolignosum rectificatum liquid is added to by 0.05%~0.2% (mass percent)
In above-mentioned bacillus subtilis bacterium solution, 37 DEG C of Yu, 7~9h of isothermal vibration culture in 180r/min incubator；Gained yellowish-brown
Bacterium solution is wood vinegar bacillus subtilis bacterium solution, and viable count is 1.7 × 109~1.8 × 109log10CFU/mL；
Mass percent shared by soil conditioner raw material are as follows: bacillus subtilis bacterium solution 99.8% -99.95%, acetum pyrolignosum rectificatum liquid
0.05%—0.2%；Mixture.
2. a kind of wood vinegar bacillus subtilis bacterium solution soil conditioner preparation according to claim 1, which is characterized in that institute
It states the activation method of bacillus subtilis: all glass being implemented into vessel and LB liquid medium carries out high pressure sterilization, use alcohol
Wipe aseptic operating platform and with ultraviolet radiator radiation sterilization；And it operates as follows:
(1) it activates the first generation: 100 ml LB liquid mediums is poured into respectively in four test tubes, it is withered that each test tube is inoculated with a needle
Careless bacillus carries out isothermal vibration (37 DEG C of 180r/min) culture for 24 hours；
(2) activate the second generation: 100 ml LB liquid mediums of production are poured into respectively in four test tubes, in every absorption generation
500 μ L bacterium solutions carry out isothermal vibration (37 DEG C of 180r/min) culture for 24 hours；
(3) second generation amplification cultivation also can be regarded as the activation third generation: 300 ml LB liquid mediums of production draw second generation bacterium solution
6mL carries out isothermal vibration culture (180r/min37 DEG C)；The first generation of activation, the second generation are put into -80 DEG C of refrigerators save it is standby
With；The third generation is implemented as activated bacillus subtilis.
3. a kind of wood vinegar bacillus subtilis bacterium solution soil conditioner according to claim 1 and its preparation, feature exist
In, the acetum pyrolignosum rectificatum liquid the preparation method comprises the following steps:
A. wooden stick is placed in closed anoxybiotic drying in drying kettle, and the wooden stick prepared is put into carbonization kettle, dry through four stages
It evaporates pyrolysis, the cooling gas-liquid separation of three-level, distill obtained crude pyroligneous acid；The liquid that gas-liquid separation is gone out, i.e. oil liquid (burnt oil and vinegar
Acid) it is transported in distillation reactor, it is crude pyroligneous acid after distillation；
B. the crude pyroligneous acid is conveyed into distiller, gaseous material is conveyed into rectifier, and rectifier will be after above-mentioned rectifying
Gaseous material is conveyed into condenser and becomes liquid, as acetum pyrolignosum rectificatum liquid.
4. a kind of wood vinegar bacillus subtilis bacterium solution soil conditioner application method, which is characterized in that the method is applied to soil
It can spray or fill in earth and apply；150-160 kilograms of modifying agent is preferably applied per acre.
5. a kind of wood vinegar bacillus subtilis bacterium solution soil conditioner application method according to claim 4, feature exist
In the preparation including toothed oak wood wood vinegar biological modification agent: drying toothed oak wood sawdust extrusion forming is put into destructive distillation by the application method
It is heated in kettle, cooling water is passed through in condensing unit, collect wood vinegar, by filtering, distillation, biological treatment；It then will be wooden
The bottling of vinegar liquid biological modification dilution agent is spare.
6. a kind of wood vinegar bacillus subtilis bacterium solution soil conditioner application method according to claim 4, feature exist
In, the application method, application program and parameter apolegamy are as follows: soil ratio 1:5 extracts soil extraction, and taking-up 30mL is molten
Liquid is placed in small beaker, is evaporated drying measurement total salt；The solution such as 20mL, 25mL measurement soil ion is taken to calculate SAR, ESP；
Soil ratio 1:1 dissolves soil, measures pH value；Potassium bichromate-concentrated sulfuric acid oxidation, additional oil bath pan boil, and measure soil
Organic matter variation, measures the physicochemical property of soil；2mm sieve 250g soil was taken, 25mL wood vinegar dilution is added, was stirred
It is uniformly fitted into beaker, is placed under same external environment and cultivates, the dilution of addition in three days takes a soil test for one week
Analysis.
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Application publication date: 20190920 |