CN103416637A - Silaged forage grass preservative and preparation method thereof - Google Patents
Silaged forage grass preservative and preparation method thereof Download PDFInfo
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- CN103416637A CN103416637A CN2013103833972A CN201310383397A CN103416637A CN 103416637 A CN103416637 A CN 103416637A CN 2013103833972 A CN2013103833972 A CN 2013103833972A CN 201310383397 A CN201310383397 A CN 201310383397A CN 103416637 A CN103416637 A CN 103416637A
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Abstract
The invention discloses silaged forage grass preservative and a preparation method of the silaged forage grass preservative. The preservative comprises trichoderma crude extracts. The silaged forage grass preservative further comprises the following components, by weight, 1-3% of nisin, 2-6%o of chitosan, 1-2%o of antioxidant of bamboo leaves and 1-5% of compound phosphate, wherein the compound phosphate is composed of 29% of sodium tripolyphosphate, 55% of sodium metaphosphate, 3% of sodium pyrophosphate and 13% of sodium dihydrogen phosphate. The silaged forage grass preservative is free of any chemical preservation component, processed silaged forage grass is almost free of contaminating microorganism growth, edible safety is high, forage grass is not polluted, the problems that the forage grass is prone to going moldy, rotting and being wasted in the silaged process at present are solved, the problem of keeping green grass fresh in the storage and transportation process is solved, and the preservative has wide promotional value.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of blue or green storage forage grass anticorrisive agent and preparation method thereof.
Background technology
In the animal husbandry breeding process, the forage grass of hoarding some is a difficult problem of guaranteeing that animal health survival, the seasonal growth of solution herbage bring.After the herbage harvesting, generally take the mode of airing, pulverizing or cellar fermentation to be stored.But the herbage after dehydration can not show a candle to being of high nutritive value of green grass.For reducing the loss of forage nutrition material, improve the cattle and sheep palatability, the blue or green storage of forage grass is a main trend of animal husbandry development.
At present for solving the blue or green storage of a forage grass difficult problem, the general mode of building the cellar for storing things, storehouse that adopts is stored, under aerobic, anaerobic condition, the microorganism Fast Growth, secrete a large amount of cellulases and carry out decomposition of cellulose, proteases for decomposing albumen produces free amino acid, produce a large amount of metabolite lactic acid and a small amount of ethanol etc. to reach anticorrosion purpose, but processing technology is loaded down with trivial details and fresh-keeping effect is not good simultaneously.A difficult problem that how to solve the blue or green storage of forage grass is a great problem that animal husbandry development faces.The present invention utilizes the secondary metabolite of wooden mould generation and the blue or green storage of a kind of forage grass anticorrisive agent that the other biological active material carries out composite acquisition, has solved the difficult problem of the blue or green storage of forage grass, has high economic benefit and society generalization and is worth.
The mould Antagonistic Fungus as phytopathogen of wood, have important biological control effect, and its antimycotic metabolite is just 70 kinds of left and right.5,6-dihydro-6-amyl group-2H-pyran-2-one (5,6-dihydro-6-pentyl-2H-pyran-2-one) be a kind of secondary metabolite with coconut fragrance that Trichoderma produces, biologically active is remarkable, and the plurality of plant diseases such as wheat sharp eyespot and cotton seedling blight are had to significant prevention effect.Its antifungal mechanism is to destroy the hyphal cell wall, and intracellular matter is exosmosed, and causes the plasm cohesion of mycelia, and the mycelia fracture is disintegrated.This biological nature of the pyrones that the present invention utilizes wooden mould fermentation to produce, processed rear storage to forage grass, through the contrast experiment, extended the green grass or young crops storage phase of forage grass, reduced the mouldy, rotten of forage grass, improved the forage grass quality.
Summary of the invention
Technical problem to be solved by this invention is that the present invention also provides the preparation method of the blue or green storage of this feed anticorrisive agent in order to solve mouldy, the rotten problem of the blue or green storage of feed, a kind of greenfeed anticorrisive agent to be provided.
To achieve these goals, the present invention proposes following technical scheme: the blue or green storage of a kind of feed is anticorrosion, the mould extract of wood, and, following component by weight percentage: nisin 1-3%, shitosan 2～6 ‰, AOB 1～2 ‰, composite phosphate (sodium phosphate trimer 29%, sodium metaphosphate 55%, sodium pyrophosphate 3%, sodium dihydrogen phosphate 13%) 1～5%.
The present invention utilizes 5 in trichoderma viride LTR-2 zymotic fluid, 6-dihydro-6-amyl group-2H-pyran-2-one, add through extracting after concentrated the greenfeed anticorrisive agent that bioactivator obtains, wherein do not add any chemical preservation composition, utilize pyrones itself to the bacteriostasis of mould and other pathogenic bacteria reach make herbage fresh-keeping, antibacterial anticorrosion, extend preservation term, reduce to greatest extent the loss of nutriment in feed, improve feed quality, guarantee the feed safety edible purpose.
The preparation method of the blue or green storage of feed provided by the invention anticorrisive agent comprises the following steps:
1, the preparation of wooden mould crude extract
(1) by trichoderma viride LTR-2 inoculation PDB culture medium, 28 ℃ of shaking tables are cultivated, and rotating speed is that 180r/min cultivates 2-4d;
(2) zymotic fluid after 30-60 minute, adds a small amount of flocculant protein precipitation through ultrasonic processing, and the diatomite that adds 0.5-1% is that then filter aid is crossed and filtered out mycelium and Partial Protein through vacuum drum;
(3) above-mentioned processing after fermentation liquid adds 30% butyl acetate extraction, then extracts anhydrous sodium sulfate dehydration through 3% NaHCO buffer solution, charcoal absorption, filter, finally the treatment fluid reduced pressure concentration is obtained to the mould crude extract of wood containing 5,6-dihydro-6-amyl group-2H-pyran-2-one;
(4) gas chromatograph-mass spectrometer (GC-MS) quantitatively detects the pyrones extracted;
2, anticorrisive agent is composite
According to testing result, with pure water, by the mould crude extract dilution of wood, be the aqueous solution that pyrones content is 0.2-0.5mg/L, add nisin 1-3%, shitosan 2～6 ‰, AOB 1～2 ‰, composite phosphate (sodium phosphate trimer 29%, sodium metaphosphate 55%, sodium pyrophosphate 3%, sodium dihydrogen phosphate 13%) 1～5%, fully mix.
3, the spraying effect control experiment of anticorrisive agent
1000 times of anticorrisive agent dilute with waters evenly are sprayed on to herbage surface to be gathered in, after within 1-2 hour, drying, are gathered in, tie up;
Herbage after processing is put at different temperature and places and observe from undressed herbage.
The specific embodiment
Below by embodiment, technical scheme of the present invention is described further, but can not be interpreted as limitation of the present invention.
The trichoderma viride LTR-2 that present embodiment is used is given by Shandong Scientific Research Academy.Makings connection spectrometer device: model is Agilent 6890-5973N GC-MS; Testing conditions is: ion gun is EI, electron energy is 70eV, and ion source temperature is 230 ℃, and interface temperature is 280 ℃, the mass scanning scope is 29～500amu, chromatographic column is HP-5MS(30m * 0.25mm * 0.25 μ m), 50 ℃ of initial column temperatures, be warmed up to 300 ℃ with 5 ℃/min after constant temperature 5min, temperature of vaporization chamber is 300 ℃, carrier gas is helium, and split ratio is 10:1, and sample size is 1 μ L.
Embodiment 1
The preparation of the mould crude extract of wood:
(1) by trichoderma viride LTR-2 inoculation PDB culture medium, connecing the bacterium amount is that 0.5%, 28 ℃ of shaking table is cultivated, and rotating speed is that 180r/min cultivation 4d is the highest to the mycelia amount;
(2) zymotic fluid after 30 minutes, adds a small amount of flocculant protein precipitation through ultrasonic processing, and adding 0.5% diatomite is that then filter aid is crossed and filtered out mycelium and Partial Protein through vacuum drum;
(3) above-mentioned processing after fermentation liquid adds 30% butyl acetate extraction, then extracts anhydrous sodium sulfate dehydration through 3% NaHCO buffer solution, charcoal absorption, filter, finally the treatment fluid reduced pressure concentration is obtained to the mould crude extract of wood containing 5,6-dihydro-6-amyl group-2H-pyran-2-one;
(4) gas chromatograph-mass spectrometer (GC-MS) is detected the pyrones extracted.Adopt areas of peak normalization method to determine the percentage composition position 0.35mg/ml of 5,6-dihydro-6-amyl group-2H-pyran-2-one;
Anticorrisive agent composite: according to testing result, with pure water, by the mould crude extract dilution of wood, be the aqueous solution that pyrones content is 2mg/L, add nisin 1.5%, shitosan 2 ‰, AOB 1 ‰, composite phosphate (sodium phosphate trimer 29%, sodium metaphosphate 55%, sodium pyrophosphate 3%, sodium dihydrogen phosphate 13%) 3%, fully mix.
The spraying effect control experiment of anticorrisive agent
Anticorrisive agent is diluted with water to 100 times and evenly is sprayed on herbage surface to be gathered in, after within 1 hour, drying, gathered in, tie up;
Herbage after processing and undressed herbage are placed in respectively to-20 ℃, 4 ℃, 10 ℃, 25 ℃, 30 ℃ placement observations;
Forage grass after observation sprays anticorrisive agent of the present invention all do not go mouldy after 30 days, rot, and the blank group that does not spray this anticorrisive agent raises along with temperature, and rotten efficiency progressively raises, all fully rotten after 30 days.Visible anticorrisive agent of the present invention is remarkable for the preservation and effect that extends blue or green storage herbage.
Embodiment 2
The place that this embodiment is different from embodiment 1 is: 1, the preparation of wooden mould crude extract (2) zymotic fluid carries out sedimentation and filtration through ultrasonic processing after 60 minutes.2, the composite middle interpolation nisin 2% of anticorrisive agent, shitosan 4 ‰, AOB 2 ‰, composite phosphate (sodium phosphate trimer 29%, sodium metaphosphate 55%, sodium pyrophosphate 3%, sodium dihydrogen phosphate 13%) 2%, fully mix.3, the spraying effect control experiment of anticorrisive agent: (1) is diluted with water 100 times by anticorrisive agent and evenly is sprayed on herbage surface to be gathered in, and after within 1 hour, drying, is gathered in, ties up.(2) herbage after processing and undressed herbage are placed in respectively to-20 ℃, 4 ℃, 10 ℃, 25 ℃, 30 ℃ placements 30 days, observed result is as follows: the forage grass after observation sprays anticorrisive agent of the present invention does not all go mouldy after 30 days, rots, and the blank group that does not spray this anticorrisive agent raises along with temperature, rotten efficiency progressively raises, all fully rotten after 30 days.Visible anticorrisive agent of the present invention is remarkable for the preservation and effect that extends blue or green storage herbage.
Claims (5)
1. a green grass or young crops stores up forage grass anticorrisive agent and preparation method thereof, it is characterized in that, the mould crude extract of wood, and, following component by weight percentage: nisin 1-3%, shitosan 2～6 ‰, AOB 1～2 ‰, composite phosphate (sodium phosphate trimer 29%, sodium metaphosphate 55%, sodium pyrophosphate 3%, sodium dihydrogen phosphate 13%) 1～5%.
2. the blue or green storage of a kind of forage grass according to claim 1 anticorrisive agent and preparation method thereof, is characterized in that, comprises altogether the preparation of wooden mould crude extract and composite two large steps of anticorrisive agent.
3. a kind of forage grass green grass or young crops according to claim 1 stores up anticorrisive agent and preparation method thereof, it is characterized in that, the preparation process of the mould crude extract of wood is:, by trichoderma viride LTR-2 inoculation PDB culture medium, 28 ℃ of shaking tables are cultivated (1), and rotating speed is that 180r/min cultivates 2-4d; (2) zymotic fluid after 30-60 minute, adds a small amount of flocculant protein precipitation through ultrasonic processing, and the diatomite that adds 0.5-1% is that then filter aid is crossed and filtered out mycelium and Partial Protein through vacuum drum; (3) above-mentioned processing after fermentation liquid adds 30% butyl acetate extraction, then extracts anhydrous sodium sulfate dehydration through 3% NaHCO buffer solution, charcoal absorption, filter, finally the treatment fluid reduced pressure concentration is obtained to the mould crude extract of wood containing 5,6-dihydro-6-amyl group-2H-pyran-2-one; (4) gas chromatograph-mass spectrometer (GC-MS) quantitatively detects the pyrones extracted.
4. a kind of forage grass green grass or young crops according to claim 1 stores up anticorrisive agent and preparation method thereof, the composite step that it is characterized in that anticorrisive agent is: according to testing result, with pure water, by the mould crude extract dilution of wood, be the aqueous solution that pyrones content is 0.2-0.5mg/L, add nisin 1-3%, shitosan 2～6 ‰, AOB 1～2 ‰, composite phosphate (sodium phosphate trimer 29%, sodium metaphosphate 55%, sodium pyrophosphate 3%, sodium dihydrogen phosphate 13%) 1～5%, fully mix.
5. the blue or green storage of a kind of forage grass according to claim 1 is anticorrosion and preparation method thereof, it is characterized in that the mode that adopts liquid dosage form directly to spray.
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| CN2013103833972A CN103416637A (en) | 2013-08-29 | 2013-08-29 | Silaged forage grass preservative and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106721891A (en) * | 2016-12-23 | 2017-05-31 | 五河县盛源畜牧养殖专业合作社 | A kind of poultry greenfeed processing harmful bacteria growth inhibition additive |
| CN107897632A (en) * | 2017-11-10 | 2018-04-13 | 雷笑天 | A kind of preparation method of high-moisture herbage bundle natural fungicidal agent |
| CN110050886A (en) * | 2019-05-15 | 2019-07-26 | 北京波尔莱特饲料有限公司 | A kind of dry and wet mixing complete feed of long shelf-life and preparation method thereof |
| CN112006174A (en) * | 2020-08-21 | 2020-12-01 | 安徽绿保源养殖有限公司 | Preparation method of preservative for feed |
-
2013
- 2013-08-29 CN CN2013103833972A patent/CN103416637A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106721891A (en) * | 2016-12-23 | 2017-05-31 | 五河县盛源畜牧养殖专业合作社 | A kind of poultry greenfeed processing harmful bacteria growth inhibition additive |
| CN107897632A (en) * | 2017-11-10 | 2018-04-13 | 雷笑天 | A kind of preparation method of high-moisture herbage bundle natural fungicidal agent |
| CN110050886A (en) * | 2019-05-15 | 2019-07-26 | 北京波尔莱特饲料有限公司 | A kind of dry and wet mixing complete feed of long shelf-life and preparation method thereof |
| CN112006174A (en) * | 2020-08-21 | 2020-12-01 | 安徽绿保源养殖有限公司 | Preparation method of preservative for feed |
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Application publication date: 20131204 |