CN103525741A - Complex microbial inoculant for greenhouse vegetable production and preparation and application thereof - Google Patents
Complex microbial inoculant for greenhouse vegetable production and preparation and application thereof Download PDFInfo
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- CN103525741A CN103525741A CN201310524057.7A CN201310524057A CN103525741A CN 103525741 A CN103525741 A CN 103525741A CN 201310524057 A CN201310524057 A CN 201310524057A CN 103525741 A CN103525741 A CN 103525741A
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Abstract
The invention discloses a complex microbial inoculant for greenhouse vegetable production and preparation and application of the complex microbial inoculant. The complex microbial inoculant is composed of mixed cultures and additives, and the mixed cultures are composed of trichoderma koningii, mucor racemosus and Bacillus subtillis. A purified and rejuvenated trichoderma koningii test tube slant culture medium, a purified and rejuvenated mucor racemosus test tube slant culture medium and a purified and rejuvenated Bacillus subtillis test tube slant culture medium are inoculated to corresponding liquid culture media respectively, and liquid bacterium seeds are manufactured through liquid fermentation; the three kinds of fermented bacterium liquid are inoculated to solid culture materials, are cultured for 48-72 hours at 28-30 DEG C and are manufactured into the mixed cultures after being dried and smashed; the additives are formed by glucose, urea and humic acid, and the mixed cultures and the additives are mixed and manufactured into the complex microbial inoculant with the weight ratio of 2.0-5.0:1.0. The mixed cultures and the additives are mixed in use, the good cooperation function is achieved, the growth and reproduction capacity and the enzyme production capacity of flora are promoted, and straw degradation is accelerated. The quality of greenhouse soil is improved, the harm of nematodes is reduced, and compared with the average product with an applied single microbial inoculant, the average product with the applied complex microbial inoculant is improved by 5-8%.
Description
Technical field
The present invention relates to the composite fungus agent in microbial technology field, particularly improve greenhouse vegetable soil quality, the composite fungus agent of producing for facilities vegetable and the application method of preparation and this microbial inoculum thereof.
Background technology
China Shi Yige large agricultural country, approximately 800,000,000 tons of every annual output agricultural crop straws.Owing to wherein containing a large amount of Mierocrystalline celluloses, be of low nutritive value, be difficult to be utilized by livestock and poultry, therefore only have and be used as on a small quantity feed, the overwhelming majority is dropped or burns, and causes pollution and the waste to resource of environment.Straw-returning is that China implements fertile soil engineering, improve constantly farmland quality, realize the important measures of agricultural sustainable development, also be the effective ways that solve crop straw burning problem, but simple directly chopping and returning, can bring disadvantageous effect to agriculture production. in going back field process, adding straw degradative bacterium is the effective ways that address this problem.
Along with the production development of facility cultivation vegetables, continuous adding straw degradative bacterium in going back field process, due to continuous cropping continuous cropping for many years, the soil organism declines, the consequent is exactly the accumulation increase of disease, worm, bacterium, as root rot, climing rot, sclerotium disease, blight and root knot nematode disease etc., cause the melon dish underproduction or withered; If reach the effect of ideal with number of chemical pesticide spraying, fumigation, pouring also difficulty, and cost is higher, again physical environment is produced and is polluted; And continuous cropping plantation can bring a lot of disease and pests, these disease and pests overwhelming majority is soil-borne disease, occurs comparatively generally in warm canopy melon dish is produced, and is also one of current world agriculture soil-borne disease insect pest difficult problem.Adopt physical method cannot thoroughly remove root knot nematode; Chemical agent easily develops immunity to drugs to root knot nematode, has residually after use, easily soil is polluted; Soil electricity sterilization operation inconvenience, price is higher.Therefore, find a kind of thoroughly effectively, health environment-friendly, easy and simple to handle, with low cost medicine or the method that can well prevent and treat root knot nematode when accelerating straw degradative be necessary.
In going back field process, adding straw decomposing inoculant is the effective ways that address this problem, in application straw decomposing inoculant, add again activator also can accelerate straw decomposing speed, but this using method is loaded down with trivial details, therefore be badly in need of finding a kind of using method simple, peasant is easy to the product of accepting, and this product can be accelerated straw degradative can prevention and elimination of disease and pests also not affect soil quality again simultaneously.
Summary of the invention
The object of the invention is to provide a kind of greenhouse vegetable soil quality, the composite fungus agent of producing for facilities vegetable and application method of preparation and this microbial inoculum thereof of improving.This composite fungus agent can not only improve the degradation speed of stalk effectively, and this composite fungus agent be manured into soil after by the continuous growth and breeding of viable bacteria, the metabolite producing (or parasitic) can also kill root-knot nematode egg, thereby reduce the harm of root knot nematode, and improve soil aggregate, raising utilization rate of fertilizer, reach the effect of increasing both production and income.
For achieving the above object, the present invention grows again to gathering in the crops underground this composite fungus agent of Straw Amendment that is embedded in whole process from planting to for facility cultivation crop, this composite fungus agent is a kind of three kinds of good single cultures of stalk effect that become thoroughly decomposed to be combined, add again additive, after mixing, become a kind of product, make to produce synergy after mixed bacterial in composite fungus agent and additive combination, simultaneously under membrane covering condition, can make stalk degrade and become thoroughly decomposed fast, not only changed the microbial biomass of soil, improved the content of soil enzyme, and increase substantially the output of vegetables, control for root knot nematode simultaneously has good effect, simple to operate, peasant uses more convenient.
The present invention proposes following technical scheme: a kind of composite fungus agent of producing for facilities vegetable, this composite fungus agent is comprised of mixed strains and additive, it is characterized in that: described mixed strains is mould by healthy and free from worry wood
(Trichoderma koningii), Mucor racemosus
(Mucor racemosus)and Bacillus subtillis
(Bacillus cereus.Frankland)form; The healthy and free from worry wood that the purification and rejuvenation is good is mould, Mucor racemosus, Bacillus subtillis test tube slant substratum are inoculated in respectively in corresponding liquid nutrient medium, through liquid fermenting, make respectively liquid spawn;
The mould substratum weight part of healthy and free from worry wood forms: Semen Maydis powder 2.0, yeast extract paste 1.0, potassium primary phosphate 0.05, sal epsom 0.05, calcium chloride 0.01, water 100, pH nature;
Bacillus subtilis bacterium culture medium weight part forms: glucose 2.0, peptone 1.5, sodium-chlor 0.5, extractum carnis 0.05, agar 2.0, water 100, pH nature;
Mucor racemosus substratum weight part forms: peptone 3.0, Zulkovsky starch 0.4, sal epsom 0.2, potassium primary phosphate 0.2, water 100, pH nature;
Three kinds of healthy and free from worry wood of liquid spawn that above-mentioned fermentation is made are mould, subtilis and Mucor racemosus are inoculated in solid culture medium, cultivate 48 ~ 72 hours in 28-30 ℃, then dry, pulverize under 50 ~ 60 ℃ of conditions, make mixed strains standby;
The weight part of described solid culture medium forms: wheat bran 55～75, and Semen Maydis powder 5～15, dregs of beans 3.0, rice husk 15～25, potassium primary phosphate 0.1, ammonium sulfate 1.0, the weight ratio of solid culture medium and water is 1:0.5～0.6;
Described additive is comprised of glucose, urea and humic acids, by glucose: urea: it is standby that humic acids is made 60～80 order particles according to the weight ratio ground and mixed of 3:1:0.1 after evenly;
The preparation of composite fungus agent: after the mixed strains preparing is mixed according to the weight ratio of 2.0～5.0:1.0 with additive, pack, the shady and cool dry place of sealing preserve.
Three kinds of healthy and free from worry wood of liquid spawn that described fermentation is made are mould: subtilis: after Mucor racemosus mixes according to the weight proportion of 1.2:1.0:0.8, by the weight ratio of liquid spawn and solid culture medium 1:10, be inoculated in immediately in solid culture medium, in 28 ~ 30 ℃ of cultivations 48 ~ 72 hours, make solid spawn; Again solid spawn is inoculated in solid culture medium to solid spawn: the weight ratio of solid culture medium is 1:10, in 28-30 ℃ of cultivation 48 hours, and dry, pulverize under 50 ~ 60 ℃ of conditions, make mixed strains standby.
The application process of composite fungus agent of the present invention is: by the weight ratio of water: composite fungus agent=10:1, soak in advance 30 minutes before using, during every 10 minutes, stir once; Ditching paving stalk in canopy, stalk usage quantity is 2000 kgs/acre, water pipe, in the watering of stalk surface, drenches stalk surface; Again soaked composite fungus agent suspension is evenly sprayed in stalk surface, earthing, overlay film, transplantation of seedlings by 0.2～0.3% of stalk weight.
The present invention has following principal feature:
1, composite fungus agent solid state fermentation, sectional material supplementing, produces enzyme stable, and the peak value length of holding time, guarantees that product enzyme system forms and enzyme is alive;
2, mixed strains mixes with additive to use and has good synergy, promotes the growth and breeding of flora, strengthens flora enzymatic productivity, accelerates straw degradative, and there will not be, mutually suppresses phenomenon between bacterial classification;
3, mixed strains adds after additive, the metabolite that flora (or parasitic) produces in continuous growth and breeding process can kill root-knot nematode egg and larva, reduce the harm of nematode, increasing both production and income, uses composite fungus agent and compares mean yield raising 5 ~ 10% with single administration activator;
4, the straw degradative enzyme such as microbial inoculum cellulase, hemicellulase, proteolytic enzyme etc. form stable, synergy significantly, fast degradation stalk, accelerate mineral and the humify of stalk, increase facility soil organic matter content, be beneficial to plant and absorb, improve soil quality.
Embodiment
One, composite fungus agent forms and preparation:
1, the composition of liquid spawn and preparation:
The healthy and free from worry wood that the purification and rejuvenation is good is mould
(Trichoderma koningii),mucor racemosus
(Mucor racemosus),bacillus subtillis
(Bacillus cereus.Frankland)test tube slant substratum is inoculated in respectively in corresponding liquid nutrient medium, through liquid fermenting, makes respectively liquid spawn;
The weight of the mould substratum of healthy and free from worry wood forms: Semen Maydis powder 2.0g, yeast extract paste 1.0g, potassium primary phosphate 0.05g, sal epsom 0.05g, calcium chloride 0.01g, water 100ml, pH nature.
The weight of bacillus subtilis bacterium culture medium forms: glucose 2.0g, peptone 1.5g, sodium-chlor 0.5g, extractum carnis 0.05g, agar 2.0g, water 100ml, pH nature.
The weight of Mucor racemosus substratum forms: peptone 3.0g, Zulkovsky starch 0.4g, sal epsom 0.2g, potassium primary phosphate 0.2g, water 100ml, pH nature.
2, the composition of mixed strains and preparation:
Three kinds of healthy and free from worry wood of liquid spawn that above-mentioned fermentation is made are mould: subtilis: after Mucor racemosus mixes according to the weight proportion of 1.2:1.0:0.8, by the liquid spawn mixing and the weight ratio of solid culture medium 1:10, be inoculated in immediately in solid culture medium, and in 28-30 ℃ of cultivation 48 ~ 72 hours, make solid spawn; Again the solid spawn obtaining is inoculated in solid culture medium and (expands numerous production), solid spawn: the weight ratio of solid culture medium is 1:10, in 28 ~ 30 ℃ of cultivations 48 hours, and dry, pulverize under 50 ~ 60 ℃ of conditions, make mixed strains standby;
3, solid culture medium forms and proportioning: (preparation solid spawn is identical with the solid culture medium that expands numerous production use)
formula 1:wheat bran 55kg, Semen Maydis powder: 15kg, dregs of beans 3.0kg, rice husk 25kg, potassium primary phosphate 0.1kg, ammonium sulfate 1.0kg, water 50 kg.
formula 2:wheat bran 60kg, Semen Maydis powder 10kg, dregs of beans 3.0kg, rice husk 25kg, potassium primary phosphate 0.1 kg, ammonium sulfate 1.0kg, water 50 kg.
formula 3:wheat bran 65 kg, Semen Maydis powder 10 kg, dregs of beans 3.0kg, rice husk 20kg, potassium primary phosphate 0.1 kg, ammonium sulfate 1.0kg, water 51 kg.
formula 4:wheat bran 75kg, Semen Maydis powder 5kg, dregs of beans 3.0 kg, rice husk 15kg, potassium primary phosphate 0.1kg, ammonium sulfate 1.0kg, water 53kg.
Solid culture preparation method for material: the wheat bran in solid material, Semen Maydis powder, dregs of beans, rice husk are taken respectively by formula ratio, mix; Then the potassium primary phosphate of proportional quantity and ammonium sulfate are added in the water that is less than total amount and dissolved, then potassium primary phosphate and ammonium sulfate solution and solid culture medium are puddled, and add the water of residual content to mix rear pack, after sterilizing, cool standby.
4, the composition of additive and preparation: it is 60～80 orders that glucose, urea, humic acids are made to granularity by the weight ratio ground and mixed of 3:1:0.1 after evenly, mixes rear standby.
5, composite fungus agent forms and preparation:
formula 5:mixed strains: additive=2.0:1.0
formula 6:mixed strains: additive=3.0:1.0
formula 7:mixed strains: additive=4.0:1.0
formula 8:mixed strains: additive=5.0:1.0
After the mixed strains preparing is mixed by above-mentioned weight ratio with additive, pack, the shady and cool dry place of sealing preserve.
Two, composite fungus agent is used
Before using, in the ratio of water: composite fungus agent=10:1, soak.Prior to ditching paving stalk in canopy, stalk usage quantity is 2000 kgs/acre, and water pipe, in the watering of stalk surface, drenches stalk surface as far as possible.Composite fungus agent adds before water extraction and soaks 30 minutes, during every 10 minutes, stir once, soaked composite fungus agent suspension is evenly sprayed in stalk surface, earthing, overlay film, transplantation of seedlings by 0.2% of stalk weight.
The present invention uses composite fungus agent and does not use the simultaneous test that composite fungus agent has carried out index of correlation facilities vegetable (take cucumber as example), uses composite fungus agent and uses single microbial inoculum and compare mean yield and improve 5 ~ 8%.Following table has been listed the correlation detection data (solid culture material assembly is selected formula 3) of using composite fungus agent of the present invention.
Remarks: preventive effect is the prevention effect to root knot nematode after use bacterial classification.
Claims (3)
1. a composite fungus agent of producing for facilities vegetable, this composite fungus agent is comprised of mixed strains and additive, it is characterized in that: described mixed strains is mould by healthy and free from worry wood
(Trichoderma koningii), Mucor racemosus
(Mucor racemosus)and Bacillus subtillis
(Bacillus cereus.Frankland)form; The healthy and free from worry wood that the purification and rejuvenation is good is mould, Mucor racemosus, Bacillus subtillis test tube slant substratum are inoculated in respectively in corresponding liquid nutrient medium, through liquid fermenting, make respectively liquid spawn;
The mould substratum weight part of healthy and free from worry wood forms: Semen Maydis powder 2.0, yeast extract paste 1.0, potassium primary phosphate 0.05, sal epsom 0.05, calcium chloride 0.01, water 100, pH nature;
Bacillus subtilis bacterium culture medium weight part forms: glucose 2.0, peptone 1.5, sodium-chlor 0.5, extractum carnis 0.05, agar 2.0, water 100, pH nature;
Mucor racemosus substratum weight part forms: peptone 3.0, Zulkovsky starch 0.4, sal epsom 0.2, potassium primary phosphate 0.2, water 100, pH nature;
Three kinds of healthy and free from worry wood of liquid spawn that above-mentioned fermentation is made are mould, subtilis and Mucor racemosus are inoculated in solid culture medium, cultivate 48 ~ 72 hours in 28-30 ℃, then dry, pulverize under 50 ~ 60 ℃ of conditions, make mixed strains standby;
The weight part of described solid culture medium forms: wheat bran 55～75, and Semen Maydis powder 5～15, dregs of beans 3.0, rice husk 15～25, potassium primary phosphate 0.1, ammonium sulfate 1.0, the weight ratio of solid culture medium and water is 1:0.5～0.6;
Described additive is comprised of glucose, urea and humic acids, by glucose: urea: it is standby that humic acids is made 60～80 order particles according to the weight ratio ground and mixed of 3:1:0.1 after evenly;
The preparation of composite fungus agent: after the mixed strains preparing is mixed according to the weight ratio of 2.0～5.0:1.0 with additive, pack, the shady and cool dry place of sealing preserve.
2. according to composite fungus agent claimed in claim 1, it is characterized in that: three kinds of healthy and free from worry wood of liquid spawn that described fermentation is made are mould: subtilis: after Mucor racemosus mixes according to the weight proportion of 1.2:1.0:0.8, by the weight ratio of liquid spawn and solid culture medium 1:10, be inoculated in immediately in solid culture medium, in 28 ~ 30 ℃ of cultivations 48 ~ 72 hours, make solid spawn; Again solid spawn is inoculated in solid culture medium to solid spawn: the weight ratio of solid culture medium is 1:10, in 28-30 ℃ of cultivation 48 hours, and dry, pulverize under 50 ~ 60 ℃ of conditions, make mixed strains standby.
3. according to the application method of the composite fungus agent described in claim 1 or 2, it is characterized in that: the application process of described composite fungus agent is: by the weight ratio of water: composite fungus agent=10:1, soak in advance 30 minutes before using, during every 10 minutes, stir once; Ditching paving stalk in canopy, stalk usage quantity is 2000 kgs/acre, water pipe, in the watering of stalk surface, drenches stalk surface; Again soaked composite fungus agent suspension is evenly sprayed in stalk surface, earthing, overlay film, transplantation of seedlings by 0.2～0.3% of stalk weight.
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| CN201310524057.7A Pending CN103525741A (en) | 2013-08-12 | 2013-10-31 | Complex microbial inoculant for greenhouse vegetable production and preparation and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103865843A (en) * | 2014-02-13 | 2014-06-18 | 河北省科学院生物研究所 | Compound microbial bacteria and application thereof in prevention and treatment of vegetable fungal diseases |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106007824B (en) * | 2016-05-23 | 2019-12-27 | 泰谷生态科技集团股份有限公司 | Composite bacterial fertilizer and preparation method and application thereof |
| CN109005927A (en) * | 2018-07-11 | 2018-12-18 | 烟台市稻香商贸有限公司 | A kind of method of straw-returning |
| CN110616156B (en) * | 2019-10-22 | 2021-06-01 | 慕恩（广州）生物科技有限公司 | Trichoderma harzianum solid state fermentation culture medium, trichoderma harzianum conidium, biological agent comprising conidium and application of biological agent |
| CN111333443B (en) * | 2019-12-03 | 2021-11-26 | 内蒙古自治区农牧业科学院 | Semi-solid fermentation powder formula of compost leavening agent and preparation method thereof |
| CN111848242A (en) * | 2020-07-29 | 2020-10-30 | 福建三炬生物科技股份有限公司 | Compound microbial agent with biocontrol function and preparation method and application thereof |
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| CN102533553A (en) * | 2011-12-29 | 2012-07-04 | 湖南省微生物研究所 | Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof |
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| CN101870596B (en) * | 2010-06-12 | 2013-01-16 | 暨南大学 | Method of preparing preparation for promoting pollination and fruit setting of xeromorphic crop by Trichodermaharzianum solid fermentation |
| CN101891548B (en) * | 2010-07-21 | 2014-06-18 | 神州汉邦（北京)生物技术有限公司 | Multiple-effect microbial fertilizer prepared by three strains of bacillus licheniformis |
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Patent Citations (3)
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| CN101823906A (en) * | 2010-04-08 | 2010-09-08 | 辽宁省微生物科学研究院 | Culture activator and application thereof |
| CN102531766A (en) * | 2011-09-15 | 2012-07-04 | 北京世纪阿姆斯生物技术股份有限公司 | Microbial decomposing agent and production method thereof |
| CN102533553A (en) * | 2011-12-29 | 2012-07-04 | 湖南省微生物研究所 | Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103865843A (en) * | 2014-02-13 | 2014-06-18 | 河北省科学院生物研究所 | Compound microbial bacteria and application thereof in prevention and treatment of vegetable fungal diseases |
| CN103865843B (en) * | 2014-02-13 | 2016-03-09 | 河北省科学院生物研究所 | A kind of microbial composite bacteria and the application in control vegetable fungi disease thereof |
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Application publication date: 20140122 |