CN107019126A - A kind of bean-pod borer man-made feeds and preparation method thereof - Google Patents
A kind of bean-pod borer man-made feeds and preparation method thereof Download PDFInfo
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- CN107019126A CN107019126A CN201710317228.7A CN201710317228A CN107019126A CN 107019126 A CN107019126 A CN 107019126A CN 201710317228 A CN201710317228 A CN 201710317228A CN 107019126 A CN107019126 A CN 107019126A
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Abstract
Field is raised the present invention relates to insect, specifically provide a kind of bean-pod borer man-made feeds and preparation method thereof, using soy meal, corn flour and wheat germ powder as main nutriment in the feed, various auxiliary materials are coordinated to be prepared, the feed obtained is suitable for bean-pod borer larval feeding, and feeding effect is better than natural host, and the nutriment needed for bean-pod borer larvae development is disclosure satisfy that in feeding process, and anti-corrosion time length, the good anti-bacterial effect of the feed, entirely it is not required to change feed from generation to generation.
Description
Technical field
Raising field the present invention relates to insect, there is provided a kind of bean-pod borer man-made feeds and preparation method thereof.
Background technology
Bean-pod borer Etiella zinckenella (Treitschke) belong to Lepidoptera Lepidoptera Pyralidaes
That Pyralidae is distribution is complicated compared with wide, host, year more, the duration length of generation, generation overlap it is worldwide
Pulse family insect, the torrid zone, subtropical zone and temperature on the south 60 degree of north latitude and to the north of 45 degree of south latitude are regional to be distributed (Peng Yuanfu
.1991;Zhou Guoyou, 2008).Current bean-pod borer is distributed in China various regions, is produced in the Yellow River and Huai He River river valley and Yangtze river basin soybean
Endanger most heavy (Zhang Xiaoyi, 1957) in area.It is reported that China's soybean acreage in 2014 has 6,590,000 hm2, and what bean-pod borer was caused
It is one of the principal element of current influence soybean yields (Yin Ruifeng etc., 2015 that soybean fallen flowers, which fall pod,;Control beautiful ripple, 2014).Beanpod
Snout moth's larva is main to be caused harm bud, beanpod, beans leaf with larva.Newly hatched larvae moth food bud, green bean, cause a large amount of bud drops, fallen flowers, fall pod.3
Larva pierces moth food beans in pod after age, and beans is stung into duct, whole beanpod is all stung sky, beanpod when causing harm serious by the lighter
Faecal pellet is accumulated outside interior and channel.Aggrieved beanpod bitter, deformity, edibility reduction;Killed beanpod easily causes rotten after the rain,
So as to cause yield to be greatly lowered and low quality.The field of preventing and treating not in time, often results in " ten pods seven eat into ", the general underproduction can
Up to 30%-50%, the serious underproduction more than 70%, it has also become outstanding problem urgently to be resolved hurrily on Soybean production (big guiding principle of bavin etc.,
2014;Yu Haixia, 2013).
Bean-pod borer is a kind of agricultural pests for being difficult to prevention and control, occurs evil with disguised, locality and fulminating feature,
It is not only relevant with the biological characteristics of its own, while also by Global climate change, the renewal of agricultural chemicals and soybean varieties and plough
Change etc. the influence of mankind's activity as mode.It is reported and has 106 years till now from bean-pod borer, but its harmfulness is only increased,
And widely dispersed.Therefore, the method for probing into out the man-made feeds and indoor feeding of bean-pod borer, and life is combined based on this
The technologies such as thing chemistry, molecular biology, it is very important to find out the effective ways of preventing and treating bean-pod borer, and in order to study beanpod
Snout moth's larva, its artificial feeding just turns into one of urgent problem to be solved.
And rational man-made feeds and method for breeding are found, it is the key point of artificial feeding insect.So far, employment
The lepidopterous insects that work is raised are up to more than 556 kinds, wherein majority is important phytophagous insect, and on beet armyworm, jade
The artificial feeding of rice snout moth's larva, lopper worm etc. is closely ripe, but the research both at home and abroad on bean-pod borer artificial feeding has no so far
Report.
The content of the invention
The many disadvantages existed for prior art, the invention provides a kind of bean-pod borer man-made feeds and its preparation side
Using soy meal, corn flour and wheat germ powder as main nutriment in method, the feed, coordinate various auxiliary materials to be prepared, obtained
Feed be suitable for bean-pod borer larval feeding, feeding effect is better than natural host, disclosure satisfy that in feeding process bean-pod borer children
Nutriment needed for worm development, and anti-corrosion time length, the good anti-bacterial effect of the feed, are entirely not required to change feed from generation to generation.
The particular content of the present invention is as follows:
A kind of bean-pod borer man-made feeds, its constituent is by weight percentage:Soy meal 15.0%-20.0%;It is beautiful
Ground rice 2.0%-3.0%;Wheat germ powder 1.8%-2.2%;Dusty yeast 0.8%-1.2%;Sucrose 1.8%-2.2%;Agar
2.0%-3.0%;Auxiliary material 2.7%-3.3%, water surplus;
Each component is calculated as by weight in wherein described auxiliary material:6.5-7.0 parts of methyl hydroxybenzoate;Potassium sorbate 6.5-7.0
Part;3.0-3.5 parts of cholesterol;16.0-17.0 parts of ascorbic acid;3.0-3.5 parts of vitamin B compound;Webster salt 50.0-55.0
Part;6.5-7.0 parts of Choline Chloride;3.0-3.5 parts of cephalosporin.
Protein and amino acid needed for soy meal, wheat germ powder, dusty yeast are provided in the present invention, meet bean-pod borer growth hair
Educate;Sucrose, corn flour are added as carbohydrate source, wherein corn flour also has certain shaping effect;The effect of agar is to raising
Material shaping, and with certain moisture-keeping function, the feedstuff main material of above-mentioned composition can meet the battalion of each growth period of bean-pod borer
Supporting needs;And in auxiliary material, cholesterol must be obtained from food, but excessive cholesterol has toxicity, especially for beans
For pod snout moth's larva, inventor has found that under above-mentioned cholesterol consumption optimal effect could be obtained;Vitamin B compound, Vitamin C
Acid and Choline Chloride are vitamin, are used as the part of coenzyme to participate in organism metabolism;Addition Webster salt can supplement insect
Required all kinds of inorganic salts, content is excessively very few all to influence insect growth to go through the phase;Use methyl hydroxybenzoate, potassium sorbate and cephalo
Mycin does not interfere with growing for insect as anticorrosion and bactericidal agent, its species and content, while may insure that bean-pod borer grows
During health.
After said components proportioning is obtained, the preparation method that inventor further provides man-made feeds is as follows:
(1) soy meal of prescription amount, corn flour, wheat germ powder, dusty yeast are weighed to sterilize 2h in conventional high-pressure at 121 DEG C, is gone out
Sucrose is added after bacterium to mix as A group materials;
(2) methyl hydroxybenzoate, potassium sorbate, cholesterol, ascorbic acid, vitamin B compound, Webster salt, chlorination courage are weighed
The distilled water that alkali, cephalosporin add up water 20% is well mixed as B group materials;
(3) remainder amount distilled water adds agar such as to soak 20min;
(4) agar after step (3) is soaked pours into A groups material after boiling and persistently stirred 5 minutes, and temperature is treated in truce cooling
When being down to 50-60 DEG C, B groups are poured into and stirred;
(5) preservative film is covered after said mixture is cooled down completely in 4 DEG C of storages of refrigerator.
Temperature when wherein auxiliary material is added should be controlled at 50-60 DEG C, so as not to too high temperature cause in auxiliary material part intolerant to
The auxiliary material failure of high temperature.
In summary, the feed that the present invention is provided is suitable for bean-pod borer larval feeding, and feeding effect is better than natural host,
The nutriment needed for bean-pod borer larvae development, and anti-corrosion time length, the antibacterial effect of the feed are disclosure satisfy that in feeding process
It is really good, entirely it is not required to change feed from generation to generation, and component is easy to purchase, compound method is simple, overcomes natural feed and is difficult to obtain
And easy mouldy shortcoming, can continuously rearing bean-pod borer indoors, obtain a large amount of selected insect sources.
Embodiment
Embodiment
A kind of bean-pod borer man-made feeds, its constituent is:
Its preparation method comprises the following steps:
(1) soy meal of prescription amount, corn flour, wheat germ powder, dusty yeast are weighed to sterilize 2h in conventional high-pressure at 121 DEG C, is gone out
Sucrose is added after bacterium to mix as A group materials;
(2) methyl hydroxybenzoate, potassium sorbate, cholesterol, ascorbic acid, vitamin B compound, Webster salt, chlorination courage are weighed
The distilled water that alkali, cephalosporin add up water 20% is well mixed as B group materials;
(3) remainder amount distilled water adds agar such as to soak 20min;
(4) agar after step (3) is soaked pours into A groups material after boiling and persistently stirred 5 minutes, and temperature is treated in truce cooling
When being down to 50-60 DEG C, B groups are poured into and stirred;
(5) preservative film is covered after said mixture is cooled down completely in 4 DEG C of storages of refrigerator.
Test example
The identification of feeding effect:The bean-pod borer man-made feeds prepared with above example 1 and the raising of natural feed soybean
Effect is compared.
In the 30ml plastic measuring glass with cover that the ovum newly produced is moved into, often pipe 1, is put into 28 ± 1 DEG C of temperature, relative humidity
70% ± 10%, L:D=14:In 10 growth cabinet, raised, often located with soybean and the gained man-made feeds of embodiment 1 respectively
3 repetitions are managed, are often repeated for examination larva 30.Observation daily is once developed and survival condition, counts each worm state development duration.Treat
Larva cocoons pupate after continue to observe the development duration of pupa, after adult eclosion, female male worm single pair mating places fresh beanpod in cup
For its spawning, the cotton balls of 10wt% hydromels and B B-complex is moistened with as Adult supplement nutrient source.Observe and record daily
The survival condition and female adult egg laying amount of female male worm, until adult is dead.Analysis draws its major vital table parameter (table 2), as a result
Show, the 1st generation and the ovum in the 3rd generation, the phase of going through of pupa that man-made feeds are raised are below natural feed, precocious stage survival rate, pupa
Eclosion rate is above natural feed, and female adult average life span and male worm average life span are all higher than natural feed;What man-made feeds were raised
The female egg laying amount of list in 1st generation and the 3rd generation higher than natural feed and difference is not notable, larva go through the phase, egg hatching rate, preoviposition period with
What natural feed was raised has no significant difference.Preferably, one epoch of completion are not required to change feed the antiseptic effect of man-made feeds, and
Natural feed soybean is easily rotted, and need to be changed daily;A large amount of fresh soyabean increases need to be freezed by being raised simultaneously with natural feed soybean
Cost, and man-made feeds are prepared and not limited by environmental condition.To sum up, this research man-made feeds have more compared to natural feed soybean
Good feeding effect, disclosure satisfy that bean-pod borer grows and bred, and obtains raised growth and develops consistent test worm.
The man-made feeds of table 2 raise the life table parameters of bean-pod borer with natural feed
Note:Data represent that different letters represent to examine in p through Tukey methods after average value ± standard error, colleague's data in table
=0.05 level difference is notable.
Claims (2)
1. a kind of bean-pod borer man-made feeds, it is characterised in that:Its constituent is by weight percentage:Soy meal 15.0%-
20.0%;Corn flour 2.0%-3.0%;Wheat germ powder 1.8%-2.2%;Dusty yeast 0.8%-1.2%;Sucrose 1.8%-2.2%;
Agar 2.0%-3.0%;Auxiliary material 2.7%-3.3%, water surplus;
Each component is calculated as by weight in wherein described auxiliary material:6.5-7.0 parts of methyl hydroxybenzoate;6.5-7.0 parts of potassium sorbate;Courage
3.0-3.5 parts of sterol;16.0-17.0 parts of ascorbic acid;3.0-3.5 parts of vitamin B compound;50.0-55.0 parts of Webster salt;Chlorine
Change 6.5-7.0 parts of choline;3.0-3.5 parts of cephalosporin.
2. the preparation method of bean-pod borer man-made feeds described in claim 1, it is comprised the following steps that:
(1) weigh the soy meal of prescription amount, corn flour, wheat germ powder, dusty yeast to sterilize 2h in conventional high-pressure at 121 DEG C, after sterilizing
Sucrose is added to mix as A group materials;
(2) methyl hydroxybenzoate, potassium sorbate, cholesterol, ascorbic acid, vitamin B compound, Webster salt, Choline Chloride, head are weighed
The distilled water that p0-357 adds up water 20% is well mixed as B group materials;
(3) remainder amount distilled water adds agar such as to soak 20min;
(4) agar after step (3) is soaked pours into A groups material after boiling and persistently stirred 5 minutes, and truce cooling treats that temperature is down to
At 50-60 DEG C, B groups are poured into and stirred;
(5) preservative film is covered after said mixture is cooled down completely in 4 DEG C of storages of refrigerator.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107535676A (en) * | 2017-09-19 | 2018-01-05 | 福建农林大学 | Man-made feeds of yellow top borer and preparation method thereof |
CN108013209A (en) * | 2017-11-28 | 2018-05-11 | 中国热带农业科学院环境与植物保护研究所 | A kind of artificial fermentation's feed for being used to raise rufivena walker larva |
CN108739678A (en) * | 2018-05-29 | 2018-11-06 | 西北农林科技大学 | Tip bark beetle in angle cultivates feed and rearing method in gram |
CN108740611A (en) * | 2018-05-24 | 2018-11-06 | 河南省农业科学院植物保护研究所 | Dichocrocis punctiferalis larva artificial feed, preparation method and dichocrocis punctiferalis method for breeding |
CN108813114A (en) * | 2018-06-26 | 2018-11-16 | 河南省农业科学院植物保护研究所 | Corn borer larvae man-made feeds and its preparation method and application |
CN112471368A (en) * | 2020-11-04 | 2021-03-12 | 湖北省农业科学院植保土肥研究所 | Artificial feed for cowpea pod borer larvae and preparation method thereof |
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CN102669472A (en) * | 2012-05-30 | 2012-09-19 | 云南农业大学 | Asiatic corn borer agar-free semi-artificial feed adopting cornstalk powder as major raw materials and preparation method of feed |
CN102907577A (en) * | 2012-09-28 | 2013-02-06 | 南京农业大学 | Artificial feed of rice leaf folder and preparation method of artificial feed |
CN103110009A (en) * | 2013-02-05 | 2013-05-22 | 中国水稻研究所 | Semi-artificial feedstuff for sesamie inferens and preparation method of feedstuff |
CN103652441A (en) * | 2013-12-02 | 2014-03-26 | 中国计量学院 | Chilo suppressalis artificial feed and preparation method and application thereof |
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CN102669472A (en) * | 2012-05-30 | 2012-09-19 | 云南农业大学 | Asiatic corn borer agar-free semi-artificial feed adopting cornstalk powder as major raw materials and preparation method of feed |
CN102907577A (en) * | 2012-09-28 | 2013-02-06 | 南京农业大学 | Artificial feed of rice leaf folder and preparation method of artificial feed |
CN103110009A (en) * | 2013-02-05 | 2013-05-22 | 中国水稻研究所 | Semi-artificial feedstuff for sesamie inferens and preparation method of feedstuff |
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Cited By (8)
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CN107535676A (en) * | 2017-09-19 | 2018-01-05 | 福建农林大学 | Man-made feeds of yellow top borer and preparation method thereof |
CN108013209A (en) * | 2017-11-28 | 2018-05-11 | 中国热带农业科学院环境与植物保护研究所 | A kind of artificial fermentation's feed for being used to raise rufivena walker larva |
CN108740611A (en) * | 2018-05-24 | 2018-11-06 | 河南省农业科学院植物保护研究所 | Dichocrocis punctiferalis larva artificial feed, preparation method and dichocrocis punctiferalis method for breeding |
CN108740611B (en) * | 2018-05-24 | 2021-10-29 | 河南省农业科学院植物保护研究所 | Artificial feed for dichocrocis punctiferalis larvae, preparation method of artificial feed and dichocrocis punctiferalis breeding method |
CN108739678A (en) * | 2018-05-29 | 2018-11-06 | 西北农林科技大学 | Tip bark beetle in angle cultivates feed and rearing method in gram |
CN108813114A (en) * | 2018-06-26 | 2018-11-16 | 河南省农业科学院植物保护研究所 | Corn borer larvae man-made feeds and its preparation method and application |
CN108813114B (en) * | 2018-06-26 | 2021-07-30 | 河南省农业科学院植物保护研究所 | Artificial feed for corn borer larvae as well as preparation method and application thereof |
CN112471368A (en) * | 2020-11-04 | 2021-03-12 | 湖北省农业科学院植保土肥研究所 | Artificial feed for cowpea pod borer larvae and preparation method thereof |
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Application publication date: 20170808 |