CN108486198A - A kind of extracting method of pillworm polypeptide and its application - Google Patents
A kind of extracting method of pillworm polypeptide and its application Download PDFInfo
- Publication number
- CN108486198A CN108486198A CN201810207179.6A CN201810207179A CN108486198A CN 108486198 A CN108486198 A CN 108486198A CN 201810207179 A CN201810207179 A CN 201810207179A CN 108486198 A CN108486198 A CN 108486198A
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- China
- Prior art keywords
- pillworm
- polypeptide
- powder
- extracting method
- degreasing
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
Abstract
The invention discloses a kind of extracting method of pillworm polypeptide and its applications, additionally provide a kind of liquid feed containing polypeptide.The pillworm polypeptide extraction process generally comprises cleaning treatment, crushes degreasing, enzymolysis and extraction and freeze-drying.It is simpler than other patents in technique using present invention preparation pillworm polypeptide, and impurity is not introduced, there can be higher quality retentivity.
Description
Technical field
The present invention relates to a kind of extracting methods of polypeptide, and in particular to a kind of side extracting polypeptide from pillworm
Method.
Background technology：
Pillworm be Crustachia Isopoda roll up Staphylinidae animal roll up beetle or Chao Chong sections animal pillworm whole worm also known as mouse it is negative,
Mouse aunt, damp worm, chicken, humidogene worm etc., be unique animal for adapting to land life completely in Crustachia.
Edible protein major function is the protein obtained after supplement addition hydrolysis in food, beverage, can be mended to human body
The compound protein for being easy to absorb, amino acid are filled, human nutrition structure is can effectively improve, especially makes the nutrition of protein matter
Structure reaches balanced ratio, builds up health to indirectly reach, and promotes development, reduces the effect of morbidity.
About the extraction of pillworm polypeptide, relevant report is less, and can see from only patent：Chinese patent
There is no progress ungrease treatments in a kind of pillworm fibrinolytic proteins of CN201610920569.9 and its application, but take the later stage
It carries out after ion exchange, gel filtration etc. repeatedly chromatograph carrying out that obtained activated protein is concentrated by ultrafiltration again, cost is higher；It is same special
Profit also has a kind of dried wood-louse extracts of Chinese patent CN201510042710.5 and its application to pass through using conventional method
Using ammonium sulfate to using dialysis to remove ammonium sulfate after protein precipitation.
Invention content：
The method that the object of the present invention is to provide a kind of to extract polypeptide from pillworm, is asked with solving above-mentioned technology
Topic.Present invention process is simple, and the polypeptide extracted has higher quality.
To achieve the above object, the present invention provides following scheme：
The present invention provides a kind of method for extracting polypeptide from pillworm, includes the following steps：
(1) it after 5~10h of pillworm Nature enemy, will then clean, dry, pulverize 60~80 mesh sieve, obtain pillworm powder
End；
(2) the pillworm powder of step (1) is taken, petroleum ether is added, 20~30min of ultrasonic extraction after filtering, is placed in 35~45
It is dry in DEG C baking oven, obtain degreasing pillworm powder；
(3) the degreasing pillworm powder for taking step (2), is added the hydrochloric acid solution of pH=2, is then added pepsin, 35~
3~5h of stirring heat preservation at 38 DEG C adjusts a pH value every 1h, and filtrate is collected in filtering；
(4) filtrate of step (3) under the conditions of 3000~3500r/min is centrifuged into 15~20min, supernatant is taken, through true
Pillworm polypeptide is obtained after empty low temperature concentration and vacuum freeze drying.
Further, the mass volume ratio (g/ml) of pillworm powder and petroleum ether is 1 in the step (2)：1~3.
Further, the mass volume ratio (g/ml) of degreasing pillworm powder and hydrochloric acid solution is 1 in the step (3)：8~
12。
Further, pH value is adjusted to 1.8~2.2 in the step (3).
Further, pillworm polypeptide obtained can apply in feed.
Further, the feed includes the liquid feed containing polypeptide.
Further, the present invention also provides a kind of liquid feed containing polypeptide, include the ingredient of following parts by weight：Mouse
6~12 parts of woman's polypeptide, 4~8 parts of polysaccharides, 1~2 part of vitamin C, 3~5 parts of glucose, sodium benzoate 0.5~1
Part, 25~40 parts of deionized water.
Compared with prior art, the present invention has following advantageous effect：
(1) pillworm polypeptide of the invention passes through petroleum ether degreasing and handles before extraction, effectively reduces lipid material pair
The protective effect of other components, while required polypeptide is dissociateed with specific enzymolysis process, it is more to effectively improve pillworm albumen
The extraction efficiency of peptide；
(2) present invention is carried out dehydrating polypeptide with vacuum freeze drying using vacuum and low temperature concentration, can reach
Preferable dehydrating effect, and do not cause too macrolesion to quality in the process, original sample can be restored to by the operation of rehydration
Son；
(3) pillworm polypeptide of the invention extracts simple, mild condition, it is easy to accomplish；
(4) pillworm polypeptide has easing pain and diminishing inflammation effect, can apply in feed, by combining other ingredients, can rise
To immunity is effectively improved, promote the health-care effects such as rehabilitation.
Specific implementation mode
With reference to specific embodiment, the present invention is described in further detail.
Embodiment 1
A method of it extracting polypeptide from pillworm, includes the following steps：
(1) it after pillworm Nature enemy 7.5h, will then clean, dry, pulverize 70 mesh sieve, obtain pillworm powder；
(2) it takes the pillworm powder of step (1), is added the petroleum ether of 2 mass volume ratios, ultrasonic extraction 25min, after filtering,
It is placed in drying in 40 DEG C of baking ovens, obtains degreasing pillworm powder；
(3) the degreasing pillworm powder of step (2) is taken, the hydrochloric acid solution of 10 mass volume ratio pH=2 is added, stomach is then added
Protease, stirring heat preservation 4h at 36.5 DEG C adjust a pH value to 2 every 1h, and filtrate is collected in filtering；
(4) filtrate of step (3) is centrifuged into 18min under the conditions of 3200r/min, takes supernatant, is concentrated through vacuum and low temperature
And pillworm polypeptide is obtained after vacuum freeze drying.
Embodiment 2
A method of it extracting polypeptide from pillworm, includes the following steps：
(1) it after pillworm Nature enemy 10h, will then clean, dry, pulverize 80 mesh sieve, obtain pillworm powder；
(2) it takes the pillworm powder of step (1), is added the petroleum ether of 3 mass volume ratios, ultrasonic extraction 30min, after filtering,
It is placed in drying in 45 DEG C of baking ovens, obtains degreasing pillworm powder；
(3) the degreasing pillworm powder of step (2) is taken, the hydrochloric acid solution of 12 mass volume ratio pH=2 is added, stomach is then added
Protease, stirring heat preservation 5h at 38 DEG C adjust a pH value to 2.2 every 1h, and filtrate is collected in filtering；
(4) filtrate of step (3) is centrifuged into 20min under the conditions of 3500r/min, takes supernatant, is concentrated through vacuum and low temperature
And pillworm polypeptide is obtained after vacuum freeze drying.
Embodiment 3
A method of it extracting polypeptide from pillworm, includes the following steps：
(1) it after pillworm Nature enemy 5h, will then clean, dry, pulverize 60 mesh sieve, obtain pillworm powder；
(2) it takes the pillworm powder of step (1), is added the petroleum ether of 1 mass volume ratio, ultrasonic extraction 20min, after filtering,
It is placed in drying in 35 DEG C of baking ovens, obtains degreasing pillworm powder；
(3) the degreasing pillworm powder of step (2) is taken, the hydrochloric acid solution of 8 mass volume ratio pH=2 is added, stomach is then added
Protease, stirring heat preservation 3h at 35 DEG C adjust a pH value to 1.8 every 1h, and filtrate is collected in filtering；
(4) filtrate of step (3) is centrifuged into 15min under the conditions of 3000r/min, takes supernatant, is concentrated through vacuum and low temperature
And pillworm polypeptide is obtained after vacuum freeze drying.
Embodiment 4
A kind of liquid feed containing polypeptide, it is specific such as table 1.
Table 1：
| Liquid feed containing polypeptide | Parts by weight |
| Pillworm polypeptide | 9.0 |
| Polysaccharides | 6.0 |
| Vitamin C | 1.5 |
| Glucose | 4.0 |
| Sodium benzoate | 0.8 |
| Deionized water | 33 |
Embodiment 5
A kind of liquid feed containing polypeptide, it is specific such as table 2.
Table 2：
Comparative example 1
A method of it extracting polypeptide from pillworm, includes the following steps：
(1) it after pillworm Nature enemy 7.5h, will then clean, dry, pulverize 70 mesh sieve, obtain pillworm powder；
(2) the pillworm powder of step (1) is taken, the hydrochloric acid solution of 10 mass volume ratio pH=2 is added, stomach cardia is then added
Enzyme, stirring heat preservation 4h at 36.5 DEG C adjust a pH value to 2 every 1h, and filtrate is collected in filtering；
(3) filtrate of step (2) is centrifuged into 18min under the conditions of 3200r/min, takes supernatant, is concentrated through vacuum and low temperature
And pillworm polypeptide is obtained after vacuum freeze drying.
Comparative example 2
A method of it extracting polypeptide from pillworm, includes the following steps：
(1) it after pillworm Nature enemy 7.5h, will then clean, dry, pulverize 70 mesh sieve, obtain pillworm powder；
(2) it takes the pillworm powder of step (1), is added the petroleum ether of 2 mass volume ratios, ultrasonic extraction 25min, after filtering,
It is placed in drying in 40 DEG C of baking ovens, obtains degreasing pillworm powder；
(3) the degreasing pillworm powder of step (2) is taken, the distilled water of 10 mass volume ratio pH=2 is added, uses 1mol/LHCl
PH=4 is adjusted, room temperature extracts 12h, repeats after extracting 2 times, and 15min is centrifuged with 3000r/min；Add 1mol/LNaOH solution
PH=9 is adjusted, room temperature extracts 12h, repeats to centrifuge after extracting 2 times, it is made fully to precipitate；
(4) 18min will be centrifuged obtained by step (3) under the conditions of 3200r/min, is freeze-dried after gained precipitation dialysis 48h
Obtain pillworm polypeptide.
Pillworm polypeptide extract yield is evaluated
Preferably to prove that it is excellent that a kind of pillworm polypeptide extracting method provided by the present invention has the effect of, choose
The extracting method of embodiment 1,2,3 and comparative example 1,2 extracts pillworm polypeptide respectively, to pillworm polypeptide extract yield into
It is measured described in row following table.
Table 3：The performance test results of embodiment 1-3 and comparative example 1-2：
The result shows that：
From table 3 it is observed that more by the pillworm albumen that the pillworm polypeptide extracting method of the present invention is extracted
Peptide extract yield is higher, and is better than comparative example.Without petroleum ether degreasing handle comparative example 1 compared with Example 1, pillworm
Polypeptide extract yield is relatively low, shows that, using the method for petroleum ether degreasing processing, the pillworm polypeptide of high yield can be obtained；
Without enzymolysis process comparative example 2 compared with Example 1, pillworm polypeptide extract yield is significant lower, shows with specific
Enzymolysis process can dissociate required polypeptide, effectively improve the extraction efficiency of pillworm polypeptide.
The present invention is described by way of example above, but the present invention is not limited to above-mentioned specific embodiment, all to be based on
Any changes or modifications that the present invention is done belong to the scope of protection of present invention.
Claims (7)
1. a kind of extracting method of pillworm polypeptide, which is characterized in that include the following steps：
(1) it after 5~10h of pillworm Nature enemy, will then clean, dry, pulverize 60~80 mesh sieve, obtain pillworm powder；
(2) the pillworm powder of step (1) is taken, petroleum ether is added, 20~30min of ultrasonic extraction after filtering, is placed in 35~45 DEG C of bakings
It is dry in case, obtain degreasing pillworm powder；
(3) the degreasing pillworm powder for taking step (2), is added the hydrochloric acid solution of pH=2, is then added pepsin, 35~38 DEG C
Lower stirring keeps the temperature 3~5h, adjusts a pH value every 1h, filtrate is collected in filtering；
(4) filtrate of step (3) under the conditions of 3000~3500r/min is centrifuged into 15~20min, takes supernatant, it is low through vacuum
Pillworm polypeptide is obtained after temperature concentration and vacuum freeze drying.
2. extracting method according to claim 1, which is characterized in that pillworm powder and petroleum ether in the step (2)
Mass volume ratio (g/ml) is 1：1~3.
3. extracting method according to claim 1, which is characterized in that degreasing pillworm powder and hydrochloric acid in the step (3)
The mass volume ratio (g/ml) of solution is 1：8~12.
4. extracting method according to claim 1, which is characterized in that pH value is adjusted to 1.8~2.2 in the step (3).
5. according to application of the pillworm polypeptide in feed made from any one of Claims 1 to 4 extracting method.
6. application according to claim 5, which is characterized in that the feed is the liquid feed containing polypeptide.
7. a kind of liquid feed containing polypeptide, which is characterized in that include the ingredient of following parts by weight：Such as Claims 1 to 4
6~12 parts of any one pillworm polypeptide, 4~8 parts of polysaccharides, 1~2 part of vitamin C, 3~5 parts of glucose, benzene first
0.5~1 part of sour sodium, 25~40 parts of deionized water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810207179.6A CN108486198A (en) | 2018-03-13 | 2018-03-13 | A kind of extracting method of pillworm polypeptide and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810207179.6A CN108486198A (en) | 2018-03-13 | 2018-03-13 | A kind of extracting method of pillworm polypeptide and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108486198A true CN108486198A (en) | 2018-09-04 |
Family
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810207179.6A Pending CN108486198A (en) | 2018-03-13 | 2018-03-13 | A kind of extracting method of pillworm polypeptide and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112992276A (en) * | 2021-02-05 | 2021-06-18 | 滨州学院 | Model and method for predicting chitosan yield in preparation of pillbug at different water bath temperatures |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750016B (en) * | 2013-12-26 | 2015-11-25 | 中山大学 | A kind of liquid feed for raising Phytoseiidae Predatory Mites and preparation method thereof |
| CN106755245A (en) * | 2016-12-30 | 2017-05-31 | 潍坊医学院 | A kind of method for extraction and purification of ground bettle glycoprotein, the ground bettle glycoprotein for preparing and its application |
| CN107223810A (en) * | 2017-06-15 | 2017-10-03 | 周代伟 | The preparation method of penaeus vannamei boone feed |
-
2018
- 2018-03-13 CN CN201810207179.6A patent/CN108486198A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750016B (en) * | 2013-12-26 | 2015-11-25 | 中山大学 | A kind of liquid feed for raising Phytoseiidae Predatory Mites and preparation method thereof |
| CN106755245A (en) * | 2016-12-30 | 2017-05-31 | 潍坊医学院 | A kind of method for extraction and purification of ground bettle glycoprotein, the ground bettle glycoprotein for preparing and its application |
| CN107223810A (en) * | 2017-06-15 | 2017-10-03 | 周代伟 | The preparation method of penaeus vannamei boone feed |
Non-Patent Citations (2)
| Title |
|---|
| 赵国玉 等: "昆虫蛋白的价值、应用及提取工艺", 《环境昆虫学报》 * |
| 魏美才 等: "昆虫蛋白质资源的开发与研究进展", 《中南林学院学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112992276A (en) * | 2021-02-05 | 2021-06-18 | 滨州学院 | Model and method for predicting chitosan yield in preparation of pillbug at different water bath temperatures |
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