CN102771670B - Artificial diet for predative natural enemy insect-true bugs - Google Patents
Artificial diet for predative natural enemy insect-true bugs Download PDFInfo
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- CN102771670B CN102771670B CN201210275175.4A CN201210275175A CN102771670B CN 102771670 B CN102771670 B CN 102771670B CN 201210275175 A CN201210275175 A CN 201210275175A CN 102771670 B CN102771670 B CN 102771670B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses an artificial diet for predative natural enemy insect-true bugs. The feeding prescription comprises 2-4g of soybean flour, 15-25g of raw pig liver, 24-50g of egg yolk, 30-50ml of water, 7-12g of cane sugar, 0.1-0.3g of vitamine C, 20-50mg of compound vitamin B, and 2-4mg of gentamicin. Each biological indicator or parameter of the stinkbugs fed by the feed disclosed by the invention is mostly the same as the parameter of the stinkbugs fed by tussah pupas; the pre-oviposition period of female fed by the artificial diet is a little longer than that of the female fed by the tussah pupas, and about 4 days longer than that of the female fed by the tussah pupas; however, the feed prescription disclosed by the invention is low in cost and simple in preparation; characters of the fed stinkbugs are similar with those of the stinkbugs fed by the tussah pupas, and the artificial diet is suitable for generalization and application in the industry.
Description
Technical field
The present invention relates to a kind of man-made feeds of raising predatory natural enemy insect-stinkbug class, particularly the artificial feed formula of Candle-sticks stinkbug.
Background technology
Predatory natural enemy insect is widely used in the biological control of agriculture and forestry injurious insect, is ensureing that the output of agriculture and forestry product and quality safety and protection of the environment avoid having brought into play very large effect in the pollution of agricultural chemicals.With man-made feeds, substituting natural host or prey raises natural enemy insect and can solve that the natural enemy insect large-scale production cycle of being caused by " plantation host plant-feed prey or host-natural enemy insect obtain " is long, large and high in cost of production series of problems take up room.Especially use the man-made feeds that add composition without the insect natural enemy insect of feeding, can break away from up hill and dale a series of expensive problems that waste time and energy of bringing by obtaining insect composition.
Candle-sticks stinkbug can be controlled from multiple agriculture and forestry injurious insects such as Lepidoptera, coleoptera, Semiptera, Homoptera, Hymenopteras, is a kind of good predatory natural enemy insect.Due to the limitation of its distribution, have no the report of its man-made feeds at present abroad.The method for breeding of domestic Candle-sticks stinkbug is mainly to adopt tussah chrysalis to feed, and tussah chrysalis can be bought, and does not need oneself breeding, although the various proterties of Candle-sticks stinkbug that the method obtains are better, still has many problems:
1,, with respect to Candle-sticks stinkbug, tussah chrysalis individuality is larger, food not to the greatest extent before with regard to corrupt and can not continue to use, cause about 60% waste.
2, with a tussah chrysalis a plurality of Candle-sticks stinkbugs of feeding simultaneously, can strengthen the ratio of autotomy between Candle-sticks stinkbug individuality.
3, tussah chrysalis is more easily bought in NORTH CHINA, and south can not or be difficult to buy.This has just limited the region of raising Candle-sticks stinkbug.
4, tussah chrysalis is perishable at high temperature season, and necessary expense is higher, therefore in summer, is hard to buy.
If 5 prepare man-made feeds with tussah chrysalis hemolymph as main composition, but still can not depart from the restriction of insect composition.
Summary of the invention
For solving above-mentioned existing technical problem, an object of the present invention is to provide a kind of man-made feeds of the predatory natural enemy insect-stinkbug class of the raising without insect composition.
Man-made feeds provided by the invention, by the raw material that comprises following portions by weight, made:
Live pig liver 15-25 part;
Egg yolk 24-50 part.
The raw material that also comprises following portions by weight in the described raw material of making man-made feeds:
Soy meal 2-4 part;
Sucrose 7-12 part.
The described raw material of making man-made feeds is preferably comprised of the component of following portions by weight:
Soy meal 2-4 part;
Live pig liver 15-25 part;
Egg yolk 24-50 part;
Sucrose 7-12 part;
Vitamin C 0.1-0.3 part;
Vitamin B compound 0.02-0.05 part;
Gentamicin 0.002-0.004 part;
Water 30-50 part.
The described raw material of making man-made feeds is most preferably comprised of the component of following portions by weight:
4 parts of soy meals;
20 parts of live pig livers;
24 parts of egg yolks;
10 parts of sucrose;
0.2 part of vitamin C;
0.04 part of vitamin B compound;
0.002 part of gentamicin;
40 parts, water.
Above-mentioned live pig liver stirs into mud shape through agitator.
Above-mentioned soy meal, live pig liver and yolk provide carbohydrate component for feed.Fresh egg yolk comprises about 0.30 gram of (carbohydrate)/100 gram, and fresh live pig liver comprises about 1.55 grams of (carbohydrate)/100 gram, and dry soybean comprises about 30.0 grams of (carbohydrate)/100 gram.
Above-mentioned soy meal, live pig liver and yolk provide protein component for feed.Fresh egg yolk comprises about 16.0 grams of (protein)/100 gram, and fresh live pig liver comprises about 16.7 grams of (protein)/100 gram, and dry soybean comprises about 40.0 grams of (protein)/100 gram.
Above-mentioned soy meal, live pig liver and yolk provide lipid component for feed.Fresh egg yolk comprises about 31.6 grams of (lipid)/100 gram, and fresh live pig liver comprises about 3.1 grams of (lipid)/100 gram, and dry soybean comprises about 20.0 grams of (lipid)/100 gram.
Above-mentioned soy meal, live pig liver and yolk provide mineral composition for feed.Fresh egg yolk comprises about 1.7g gram of (mineral matter)/100 gram, and fresh live pig liver comprises about 1.4 grams of (mineral matter)/100 gram, and dry soya bean comprises approximately 95 milligrams of (mineral matter)/100 gram.
Above-mentioned sucrose provides main energy for natural enemy insect.In addition, sucrose has the food of helping effect to insect, and polyphagy insect more can produce reaction to carbohydrate compared with oligophagy insect, and sugared helps food effect relevant with concentration.
Above-mentioned vitamin B compound is the mixture of several B family vitamins.Vitamin B compound has 6 kinds of Cobastabs: (folic acid, pantothenic acid, VB1, VB2, VB3, VB12).The ratio of weight and number of described folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 is 1:3:1:3:50:6.
Said vitamin C, is again ascorbic acid, is a kind of water soluble vitamin.VC can promote the growth of low age insect, and prevent disease is conducive to improve egg laying amount, improves the production performance of raising natural enemy insect.
Above-mentioned gentamicin is a kind of aminoglycoside antibiotics, is mainly used in treating bacterium and infects, the infection that especially Gram-negative bacteria causes.Thereby be applied to prevent in man-made feeds feed mold.
Man-made feeds provided by the present invention can be for raising natural enemy insect predatory bug insect, particularly Candle-sticks stinkbug, applicable each worm state of Candle-sticks stinkbug and each length of time.Experiment showed, that the Life of Adult of feeding with man-made feeds of the present invention is longer than the life-span of the adult of feeding with tussah chrysalis, wherein longevity of male grows approximately 14 days, and the female worm life-span grows approximately 11 days.
Man-made feeds composition of the present invention source is wide, easily buys, and less investment, production cost is low, is suitable for applying in industry.
The specific embodiment
Experimental technique in following embodiment, if no special instructions, is conventional method.
Percentage composition in following embodiment, if no special instructions, is quality percentage composition.
The preparation of embodiment 1, man-made feeds
The man-made feeds of the present embodiment, by the raw material of following portions by weight ratio, made:
20 parts of live pig livers;
30 parts of egg yolks.
First be cut into live pig liver by knife fritter, then with mixer, stir into live pig Liver paste standby.According to above-mentioned ratio of weight and number, get egg yolk (life) and add live pig Liver paste to stir with agitator, with sealed membrane sealing, put into refrigerator 4 degree and preserve.Preparation is available week age once.
The preparation of embodiment 2, man-made feeds
The man-made feeds of the present embodiment, by the raw material of following portions by weight ratio, made:
15 parts of live pig livers;
50 parts of egg yolks;
3 parts of soy meals;
12 parts of sucrose.
First be cut into live pig liver by knife fritter, then with mixer, stir into live pig Liver paste standby.According to above-mentioned ratio of weight and number, get egg yolk (raw) and with glass bar, stir to open and stir evenly, then add sucrose, soy meal, live pig Liver paste to stir with agitator, with sealed membrane sealing, put into refrigerator 4 degree and preserve.Preparation is available week age once.
The preparation of embodiment 3, man-made feeds
The raw material of making man-made feeds is comprised of the component of following portions by weight ratio:
4 parts of soy meals;
20 parts of live pig livers;
24 parts of egg yolks;
10 parts of sucrose;
0.2 part of vitamin C;
0.04 part of vitamin B compound;
0.002 part of gentamicin;
40 parts, water.
The mixture that above-mentioned vitamin B compound is comprised of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, the ratio of weight and number of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 is 1:3:1:3:50:6.
Wherein, live pig liver stirs into mud shape through agitator.
Concrete preparation method is as described below:
First be cut into live pig liver by knife fritter, then with mixer, stir into live pig Liver paste standby.According to above-mentioned ratio of weight and number, get egg yolk (raw) and add with glass bar, to stir to open after water and stir evenly, then add sucrose, VC, VB, soy meal, live pig Liver paste and gentamicin, finally with agitator, stir, with sealed membrane sealing, put into refrigerator 4 degree and preserve.Preparation is available week age once.
The preparation of embodiment 4, man-made feeds
The raw material of making man-made feeds is comprised of the component of following portions by weight ratio:
The described raw material of making man-made feeds is preferably comprised of the component of following portions by weight ratio:
2 parts of soy meals;
25 parts of live pig livers;
24 parts of egg yolks;
12 parts of sucrose;
0.3 part of vitamin C;
0.05 part of vitamin B compound;
0.004 part of gentamicin;
50 parts, water.
The mixture that above-mentioned vitamin B compound is comprised of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, the ratio of weight and number of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 is 1:3:1:3:50:6.
Wherein, live pig liver stirs into mud shape through agitator.
Concrete preparation method is as described below:
First be cut into live pig liver by knife fritter, then with mixer, stir into live pig Liver paste standby.According to above-mentioned ratio of weight and number, get egg yolk (raw) and enter with glass bar, to stir to open after water and stir evenly, then add sucrose, VC, VB, soy meal, live pig Liver paste and gentamicin, finally with agitator, stir, with sealed membrane sealing, put into refrigerator 4 degree and preserve.Preparation is available week age once.
The preparation of embodiment 5, man-made feeds
The raw material of making man-made feeds is comprised of the component of following portions by weight ratio:
The described raw material of making man-made feeds is preferably comprised of the component of following portions by weight ratio:
4 parts of soy meals;
15 parts of live pig livers;
50 parts of egg yolks;
7 parts of sucrose;
0.1 part of vitamin C;
0.02 part of vitamin B compound;
0.002 part of gentamicin;
30 parts, water.
The mixture that above-mentioned vitamin B compound is comprised of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, the ratio of weight and number of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 is 1:3:1:3:50:6.
Wherein, live pig liver stirs into mud shape through agitator.
Concrete preparation method is as described below:
First be cut into live pig liver by knife fritter, then with mixer, stir into live pig Liver paste standby.According to above-mentioned ratio of weight and number, get egg yolk (raw) and enter with glass bar, to stir to open after water and stir evenly, then add sucrose, VC, VB, soy meal, live pig Liver paste and gentamicin, finally with agitator, stir, with sealed membrane sealing, put into refrigerator 4 degree and preserve.Preparation is available week age once.
The feeding effect experiment of embodiment 6, feed of the present invention
Candle-sticks stinkbug nymph in one age only provides distilled water just can grow to two ages, does not need to provide food.Experiment is carried out five groups, the man-made feeds of feeding respectively and preparing in embodiment 1-5, every group is carried out as follows: two ages of feeding, three ages, four ages, five age nymph all carry out single glass (300 milliliters of dixie cups) and raise, i.e. the every glass of Candle-sticks stinkbug of feeding, rim of a cup covers in case escape with gauze.Raise borer population amount for each processes 150 of each worm ages.Nymph every day in every of each length of time is feeding feed stuff 40 microlitres, 40 microlitres, 80 microlitres, 120 microlitres respectively.The adult (a pair of) of the pairing 320 microlitre feeds of feeding every day.Feed is the capsule shape man-made feeds with plastic sheeting and Parafilm sealed membrane plastic packaging, and every feed capsule is hemispherical, and capsule volume is 40 microlitres, and capsule is respectively the man-made feeds of preparing in embodiment 1-5, while feeding, feed is placed on gauze.Change man-made feeds every day one time.Contrast is for taking food the Candle-sticks stinkbug of tussah chrysalis, and its method for breeding is identical with the Candle-sticks stinkbug that takes food man-made feeds with raising quantity.The adult of each nymph and pairing single tussah chrysalis of feeding is changed a tussah chrysalis for 7-12 days in length of time.
Table 1. takes food Candle-sticks stinkbug body weight (milligram) and long (millimeter) (Mean ± SD) of body of tussah chrysalis and man-made feeds.
Table 2. take food Candle-sticks stinkbug nymph survival rate (%), the nymphal development of tussah chrysalis and man-made feeds go through the phase (my god), preoviposition period (my god), Life of Adult (my god) and egg laying amount (grain) (Mean ± SD).
Result shows, with the embodiment of the present invention 1 and embodiment 2 man-made feeds, Candle-sticks stinkbug can not be raised to adult, but the every biological indicator of Candle-sticks stinkbug or the parameter of with the artificial feed embodiment 3 of the present invention, embodiment 4 and embodiment 5, raising are mostly identical with the parameter of the Candle-sticks stinkbug of feeding with tussah chrysalis.Embodiment 3 feeding effects are best, with the nymphal development that embodiment 3 man-made feeds are fed, go through the nymph that the phase is slightly longer than the tussah chrysalis of feeding; Female worm preoviposition period than the female worm of feeding with tussah chrysalis preoviposition period slightly long, approximately long 4 days; The egg laying amount of female worm of embodiment 3 man-made feeds of feeding is less than the egg laying amount of the female worm of the tussah chrysalis of feeding.But the Life of Adult of feeding with embodiment 3 man-made feeds is longer than the life-span of the adult of feeding with tussah chrysalis, wherein longevity of male grows approximately 14 days, and the female worm life-span grows approximately 11 days.
Claims (4)
1. man-made feeds, is characterized in that: the raw material of making man-made feeds is comprised of the component of following portions by weight:
Soy meal 2-4 part;
Live pig liver 15-25 part;
Egg yolk 24-50 part;
Sucrose 7-12 part;
Vitamin C 0.1-0.3 part;
Vitamin B compound 0.02-0.05 part;
Gentamicin 0.002-0.004 part;
Water 30-50 part;
Described vitamin B compound is by folic acid, pantothenic acid, VB
1, VB
2, VB
3and VB
12form described folic acid, pantothenic acid, VB
1, VB
2, VB
3and VB
12ratio of weight and number be 1:3:1:3:50:6.
2. man-made feeds according to claim 1, is characterized in that: the raw material of making man-made feeds is comprised of the component of following portions by weight ratio:
4 parts of soy meals;
20 parts of live pig livers;
24 parts of egg yolks;
10 parts of sucrose;
0.2 part of vitamin C;
0.04 part of described vitamin B compound;
0.002 part of gentamicin;
40 parts, water.
3. man-made feeds according to claim 1 and 2, is characterized in that: live pig Liver Channel is crossed agitator and stirred into mud shape.
4. the application of the man-made feeds described in any one in raising predatory bug insect in claim 1-3, described predatory bug is Candle-sticks stinkbug.
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| CN103088143B (en) * | 2013-01-28 | 2014-02-05 | 中国农业科学院植物保护研究所 | Method for detecting egg laying amount of insects by spermatine gene |
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