CN102771670A - Artificial diet for predative natural enemy insect-true bugs - Google Patents
Artificial diet for predative natural enemy insect-true bugs Download PDFInfo
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- CN102771670A CN102771670A CN2012102751754A CN201210275175A CN102771670A CN 102771670 A CN102771670 A CN 102771670A CN 2012102751754 A CN2012102751754 A CN 2012102751754A CN 201210275175 A CN201210275175 A CN 201210275175A CN 102771670 A CN102771670 A CN 102771670A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention discloses an artificial diet for predative natural enemy insect-true bugs. The feeding prescription comprises 2-4g of soybean flour, 15-25g of raw pig liver, 24-50g of egg yolk, 30-50ml of water, 7-12g of cane sugar, 0.1-0.3g of vitamine C, 20-50mg of compound vitamin B, and 2-4mg of gentamicin. Each biological indicator or parameter of the stinkbugs fed by the feed disclosed by the invention is mostly the same as the parameter of the stinkbugs fed by tussah pupas; the pre-oviposition period of female fed by the artificial diet is a little longer than that of the female fed by the tussah pupas, and about 4 days longer than that of the female fed by the tussah pupas; however, the feed prescription disclosed by the invention is low in cost and simple in preparation; characters of the fed stinkbugs are similar with those of the stinkbugs fed by the tussah pupas, and the artificial diet is suitable for generalization and application in the industry.
Description
Technical field
The present invention relates to a feeding predator insects - bugs kind of artificial feed, particularly candle sticks bugs artificial feed formulation.
Background technology
The predator insect is widely used in the biological control of agriculture and forestry injurious insect, in the output that ensures agriculture and forestry product with quality safety and protect environment to avoid having brought into play very big effect in the pollution of agricultural chemicals.With man-made feeds substitute natural host or prey raise natural enemy insect can solve the natural enemy insect large-scale production cycle of being caused by " plantation host plant-feed prey or host-natural enemy insect obtain " long, a series of problems such as big and cost height take up room.Especially the man-made feeds that add composition with the no insect natural enemy insect of feeding can be broken away from a series of expensive problems that waste time and energy of being brought by acquisition insect composition up hill and dale.
Candle sticks bugs can be controlled from the Lepidoptera, Coleoptera, Hemiptera, Homoptera, Hymenoptera, and other pests, is a good predator insects.Since the limitation of its distribution, the external at present report of not seeing its man-made feeds.Domestic candle sticks stink bug feeding method is used mainly feeding silkworm pupae, silkworm pupae can be purchased without their own reproduction, although the method of candle sticks bugs get various traits better, but there are still many questions:
1, relative to the candle sticks bugs in terms of individual large silkworm pupae in food do not have corruption before it can not continue to use, resulting in approximately 60% of the waste.
2, with a silkworm chrysalis while feeding multiple candle sticks, candle sticks bugs bugs will increase the proportion of self-mutilation among individuals.
3, tussah chrysalis is prone to buy in NORTH CHINA, and the south can not or be difficult to buy.This limits the regional breeding candle sticks bugs.
4, tussah chrysalis is perishable at high temperature season, and therefore necessary expense is hard to buy in summer with higher.
If 5 use the tussah chrysalis hemolymph to prepare man-made feeds as main composition, but still can not break away from the restriction of insect composition.
Summary of the invention
For solving above-mentioned existing technical problem, an object of the present invention is to provide a kind of man-made feeds that do not have the raising predator insect-stinkbug class of insect composition.
Man-made feeds provided by the invention, by comprising that following materials based on weight processes:
Live pig liver 15-25 part;
Egg yolk 24-50 part.
Also comprise following materials based on weight in the said raw material of processing man-made feeds:
Soy meal 2-4 part;
Sucrose 7-12 part.
The said raw material of processing man-made feeds preferably is made up of the component of following portions by weight:
Soy meal 2-4 part;
Live pig liver 15-25 part;
Egg yolk 24-50 part;
Sucrose 7-12 part;
Vitamin C 0.1-0.3 part;
Vitamin B compound 0.02-0.05 part;
Gentamicin 0.002-0.004 part;
Water 30-50 part.
The said raw material of processing man-made feeds most preferably is made up of the component of following portions by weight:
4 parts of soy meals;
20 parts of live pig livers;
24 parts of egg yolks;
10 parts of sucrose;
0.2 part of vitamin C;
0.04 part of vitamin B compound;
0.002 part of gentamicin;
40 parts in water.
Above-mentioned live pig liver stirs into the mud shape through agitator.
Above-mentioned soy meal, live pig liver and yolk are that feed provides carbohydrate component.Fresh egg yolk comprises about 0.30 gram (carbohydrate)/100 gram, and fresh live pig liver comprises about 1.55 gram (carbohydrate)/100 grams, and dried soybean comprises about 30.0 gram (carbohydrate)/100 grams.
Above-mentioned soy meal, live pig liver and yolk are that feed provides protein component.Fresh egg yolk comprises about 16.0 gram (protein)/100 grams, and fresh live pig liver comprises about 16.7 gram (protein)/100 grams, and dried soybean comprises about 40.0 gram (protein)/100 grams.
Above-mentioned soy meal, live pig liver and yolk are that feed provides lipid component.Fresh egg yolk comprises about 31.6 gram (lipid)/100 grams, and fresh live pig liver comprises about 3.1 gram (lipid)/100 grams, and dried soybean comprises about 20.0 gram (lipid)/100 grams.
Above-mentioned soy meal, live pig liver and yolk are that feed provides mineral composition.Fresh egg yolk comprises about 1.7g gram (mineral matter)/100 gram, and fresh live pig liver comprises about 1.4 gram (mineral matter)/100 grams, and dried soya bean comprises about 95 milligrams of (mineral matter)/100 gram.
Above-mentioned sucrose is that natural enemy insect provides main energy.In addition, sucrose has the food of helping effect to insect, and the polyphagy insect more can produce reaction to carbohydrate than the oligophagy insect, and sugared helps the food effect relevant with concentration.
Above-mentioned vitamin B compound is the mixture of several kinds of B family vitamins.Vitamin B compound has 6 kinds of Cobastabs: (folic acid, pantothenic acid, VB1, VB2, VB3, VB12).The ratio of weight and number of said folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 is 1:3:1:3:50:6.
Said vitamin C is ascorbic acid again, is a kind of water soluble vitamin.VC can promote the growth of low age insect, and prevent disease helps improving egg laying amount, improves the production performance of raising natural enemy insect.
Above-mentioned gentamicin is a kind of aminoglycoside antibiotics, is mainly used in the treatment bacterial infection, the especially infection that causes of Gram-negative bacteria.Thereby be applied to prevent in the man-made feeds feed mold.
The present invention provides an artificial diet can be used for feeding insect predatory bugs insects, bugs especially candle sticks, candle sticks bugs for various instars and the age.Experiment showed, that the adult life-span of feeding with man-made feeds of the present invention is longer than the life-span of the adult of feeding with tussah chrysalis, wherein the male worm life-span grew about 14 days, and the female worm life-span grew about 11 days.
Man-made feeds composition of the present invention source is wide, is prone to buy, and less investment, production cost is low, is suitable in industry, applying.
The specific embodiment
Experimental technique among the following embodiment if no special instructions, is conventional method.
Percentage composition among the following embodiment if no special instructions, is the quality percentage composition.
The preparation of embodiment 1, man-made feeds
The man-made feeds of present embodiment, process by the raw material of following portions by weight ratio:
20 parts of live pig livers;
30 parts of egg yolks.
Earlier be cut into the live pig liver by knife fritter, it is subsequent use to stir into the live pig Liver paste with mixer then.Get egg yolk (giving birth to) adding live pig Liver paste according to above-mentioned ratio of weight and number and stir,, put into refrigerator 4 degree and preserve with sealing the film sealing with agitator.Prepare once available week age.
The preparation of embodiment 2, man-made feeds
The man-made feeds of present embodiment, process by the raw material of following portions by weight ratio:
15 parts of live pig livers;
50 parts of egg yolks;
3 parts of soy meals;
12 parts of sucrose.
Earlier be cut into the live pig liver by knife fritter, it is subsequent use to stir into the live pig Liver paste with mixer then.Get egg yolk (give birth to) according to above-mentioned ratio of weight and number and stir to open with glass bar and stir, add sucrose, soy meal, live pig Liver paste then and stir,, put into refrigerator 4 degree preservations with sealing the film sealing with agitator.Prepare once available week age.
The preparation of embodiment 3, man-made feeds
Processing the raw material of man-made feeds is made up of following components by weight portion:
4 parts of soy meals;
20 parts of live pig livers;
24 parts of egg yolks;
10 parts of sucrose;
0.2 part of vitamin C;
0.04 part of vitamin B compound;
0.002 part of gentamicin;
40 parts in water.
The mixture that above-mentioned vitamin B compound is made up of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, the ratio of weight and number of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 are 1:3:1:3:50:6.
Wherein, the live pig liver stirs into the mud shape through agitator.
Concrete preparation method is described below:
Earlier be cut into the live pig liver by knife fritter, it is subsequent use to stir into the live pig Liver paste with mixer then.Get egg yolk (give birth to) according to above-mentioned ratio of weight and number and add after the entry to stir to open and stir, add sucrose, VC, VB, soy meal, live pig Liver paste and gentamicin then, stir with agitator at last,, put into refrigerator 4 degree preservations with sealing the film sealing with glass bar.Prepare once available week age.
The preparation of embodiment 4, man-made feeds
Processing the raw material of man-made feeds is made up of following components by weight portion:
The said raw material of processing man-made feeds preferably is made up of following components by weight portion:
2 parts of soy meals;
25 parts of live pig livers;
24 parts of egg yolks;
12 parts of sucrose;
0.3 part of vitamin C;
0.05 part of vitamin B compound;
0.004 part of gentamicin;
50 parts in water.
The mixture that above-mentioned vitamin B compound is made up of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, the ratio of weight and number of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 are 1:3:1:3:50:6.
Wherein, the live pig liver stirs into the mud shape through agitator.
Concrete preparation method is described below:
Earlier be cut into the live pig liver by knife fritter, it is subsequent use to stir into the live pig Liver paste with mixer then.Get after egg yolk (the give birth to) entry to stir to open according to above-mentioned ratio of weight and number and stir, add sucrose, VC, VB, soy meal, live pig Liver paste and gentamicin then, stir with agitator at last,, put into refrigerator 4 degree preservations with sealing the film sealing with glass bar.Prepare once available week age.
The preparation of embodiment 5, man-made feeds
Processing the raw material of man-made feeds is made up of following components by weight portion:
The said raw material of processing man-made feeds preferably is made up of following components by weight portion:
4 parts of soy meals;
15 parts of live pig livers;
50 parts of egg yolks;
7 parts of sucrose;
0.1 part of vitamin C;
0.02 part of vitamin B compound;
0.002 part of gentamicin;
30 parts in water.
The mixture that above-mentioned vitamin B compound is made up of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, the ratio of weight and number of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 are 1:3:1:3:50:6.
Wherein, the live pig liver stirs into the mud shape through agitator.
Concrete preparation method is described below:
Earlier be cut into the live pig liver by knife fritter, it is subsequent use to stir into the live pig Liver paste with mixer then.Get after egg yolk (the give birth to) entry to stir to open according to above-mentioned ratio of weight and number and stir, add sucrose, VC, VB, soy meal, live pig Liver paste and gentamicin then, stir with agitator at last,, put into refrigerator 4 degree preservations with sealing the film sealing with glass bar.Prepare once available week age.
The feeding effect experiment of embodiment 6, feed of the present invention
Candle sticks bugs an instar nymphs only distilled water can develop to the second instar, without providing food.Experiment five groups and fed prepared in Example 1-5 artificial feed, each according to the following method: feeding second instar, third instar, fourth instar, fifth instar nymphs have carried out single-cup (300 ml cup) feeding, feeding a candle sticks per cup bugs, cup with gauze covering to prevent escape.Raise the borer population amount and be 150 of each worm of each processing ages.Every nymph every day in each length of time is feeding feed stuff 40 microlitres, 40 microlitres, 80 microlitres, 120 microlitres respectively.The adult (a pair of) of the pairing 320 microlitre feeds of feeding every day.Feed is for sealing the capsule shape man-made feeds of film plastic packaging with plastic sheeting and Parafilm, and every feed capsule is a hemispherical, and the capsule volume is 40 microlitres, and capsule is respectively the man-made feeds that prepare among the embodiment 1-5, when feeding feed is placed on the gauze to get final product.Change man-made feeds every day one time.Control of feeding silkworm pupae candle sticks bugs, their farming methods and feeding quantity fed artificial feed candle sticks with the same bug.The adult of each nymph and pairing single tussah chrysalis of feeding was changed a tussah chrysalis in 7-12 days in the length of time.
Table 1. Feeding silkworm pupae and artificial feed candle sticks bug weight (mg) and body length (mm) (Mean ± SD).
Table 2. Feeding silkworm pupae and artificial feed candle sticks bug nymph survival rate (%), nymphal developmental period (days), spawning early (days), adult longevity (days) and fecundity (tablets) (Mean ± SD).
The results show that the present invention Example 1 and Example 2 can be manually feed feeding to adult bugs candle sticks, but the present invention is artificial feed Example 3 Example 4 Example 5 and reared biological indicators of candle sticks bug silkworm chrysalis or parameter with a parameter fed candle sticks mostly the same bug.Embodiment 3 feeding effects are best, and the nymphal development of feeding with embodiment 3 man-made feeds is gone through the nymph that the phase is longer than the tussah chrysalis of feeding slightly; Female worm the preoviposition period than the female worm of feeding with tussah chrysalis the preoviposition period long slightly, approximately long 4 days; The egg laying amount of female worm of embodiment 3 man-made feeds of feeding is less than the egg laying amount of the female worm of the tussah chrysalis of feeding.But the adult life-span with embodiment 3 man-made feeds are fed is longer than the life-span of the adult of feeding with tussah chrysalis, and wherein the male worm life-span grew about 14 days, and the female worm life-span grew about 11 days.
Claims (9)
1. man-made feeds, by comprising that following materials based on weight processes:
Live pig liver 15-25 part;
Egg yolk 24-50 part.
2. man-made feeds according to claim 1 is characterized in that: also comprise following materials based on weight in the said raw material of processing man-made feeds:
Soy meal 2-4 part;
Sucrose 7-12 part.
3. man-made feeds according to claim 2 is characterized in that: the said raw material of processing man-made feeds is made up of the component of following portions by weight:
Soy meal 2-4 part;
Live pig liver 15-25 part;
Egg yolk 24-50 part;
Sucrose 7-12 part;
Vitamin C 0.1-0.3 part;
Vitamin B compound 0.02-0.05 part;
Gentamicin 0.002-0.004 part;
Water 30-50 part.
4. man-made feeds according to claim 3 is characterized in that: the said raw material of processing man-made feeds is made up of following components by weight portion:
4 parts of soy meals;
20 parts of live pig livers;
24 parts of egg yolks;
10 parts of sucrose;
0.2 part of vitamin C;
0.04 part of vitamin B compound;
0.002 part of gentamicin;
40 parts in water.
5. according to any described man-made feeds among the claim 1-4, it is characterized in that: said vitamin B compound comprises folic acid, pantothenic acid, VB1, VB2, VB3 and VB12.
6. man-made feeds according to claim 5; It is characterized in that: said vitamin B compound is made up of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, and the ratio of weight and number of said folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 is 1:3:1:3:50:6.
7. according to any described man-made feeds among the claim 1-6, it is characterized in that: said live pig liver stirs into the mud shape through agitator.
8. the application of any described man-made feeds in raising predatism stinkbug class insect among the claim 1-7.
As claimed in claim 8, characterized in that: said predatory bugs Heteroptera of candle sticks.
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| CN103060465A (en) * | 2013-01-28 | 2013-04-24 | 中国农业科学院植物保护研究所 | Method for detecting hatchability of insect eggs by using spermatin gene |
| CN103060466A (en) * | 2013-01-28 | 2013-04-24 | 中国农业科学院植物保护研究所 | Method for detecting self-mutilation rate of insects by using phylaxin gene |
| CN103088143A (en) * | 2013-01-28 | 2013-05-08 | 中国农业科学院植物保护研究所 | Method for detecting egg laying amount of insects by spermatine gene |
| CN103088141A (en) * | 2013-01-28 | 2013-05-08 | 中国农业科学院植物保护研究所 | Method for detecting egg laying amount of insects by heat shock protein 83-1 gene |
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| CN108402343B (en) * | 2018-04-09 | 2022-03-22 | 华南农业大学 | Artificial feed formula for harris baccarum |
| CN108653302A (en) * | 2018-06-28 | 2018-10-16 | 上海市第六人民医院 | B solid pharmaceutical preparations and its preparation method and application are tieed up greatly in a kind of celebrating for treating stomach trouble |
| CN108936102A (en) * | 2018-07-25 | 2018-12-07 | 中国农业科学院植物保护研究所 | A kind of Candle-sticks stinkbug man-made feeds and preparation method thereof |
| CN112385608A (en) * | 2019-08-19 | 2021-02-23 | 云南省烟草公司大理州公司 | Breeding method of lugworm |
| CN115956642A (en) * | 2022-11-30 | 2023-04-14 | 贵州省烟草科学研究院 | Armoracia chinensis artificial feed and preparation method thereof |
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