CN103271247A - Artificial feed of cyrtorhinus lividipennis - Google Patents

Artificial feed of cyrtorhinus lividipennis Download PDF

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CN103271247A
CN103271247A CN2013102180502A CN201310218050A CN103271247A CN 103271247 A CN103271247 A CN 103271247A CN 2013102180502 A CN2013102180502 A CN 2013102180502A CN 201310218050 A CN201310218050 A CN 201310218050A CN 103271247 A CN103271247 A CN 103271247A
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vitamin
parts
made feeds
green plant
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CN103271247B (en
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郑许松
何晶晶
吕仲贤
徐红星
杨亚军
朱平阳
刘桂良
陈桂华
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Zhejiang Academy of Agricultural Sciences
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Abstract

The invention discloses an artificial feed formula of cyrtorhinus lividipennis. The artificial feed of cyrtorhinus lividipennis comprises the following components: tussah pupa homogenate, egg yolk, whole milk powder, cane sugar, lecithin, rice ketone, beer yeast, vitamin C, composite vitamin B, choline chloride, nipagin and water. The artificial feed is suitable for feeding a great amount of adults and nymphs of cyrtorhinus lividipennis artificially, is high in survival rate in feeding cyrtorhinus lividipennis, is rapid in growth and development process, is high in isofemale average production, and has the characteristics of long guarantee period, time conservation and labor conservation, simplicity and easiness in operation, and high practicability.

Description

The man-made feeds of a kind of black porch green plant bug
Technical field
The present invention relates to insect and raise the field, specifically, relate to a kind of man-made feeds of raising fleahopper, more particularly, relate to a kind of man-made feeds of raising black porch green plant bug adult and nymph.
Background technology
Paddy rice is the staple food of China's 60% above population, and Rice Production rich owed the grain security that surplus is directly connected to China.Since entering this century, planthopper occurrence scope and generation quantity further rise.According to statistics, surplus 2005-2007 annual generation hazard area reaches 2,670 ten thousand hectares, reach historical high (Xia Jingyuan, 2008).Planthopper has become and has jeopardized China's grain security and the most serious biological epidemics of ecological safety.The biological control technology of crop pest has high selectivity, and is free from environmental pollution to people, animal and beneficial organism and crop safety, to sick worm advantages such as longer inhibitory action are arranged, utilizing natural enemy to come the prevention target insect is the Key Strategy of biological control.
Black porch green plant bug Cyrtorrhinus lividipennis, belong to the Semiptera Miridae, ovum and low age nymph with plant hopper and leafhopper are food, are the important predators of rice field plant hopper and leafhopper class insect, and the black porch green plant bug reaches 20-60% to the field predation rate of planthopper.But the black porch green plant bug is a kind of moving property of companion natural enemy, and with the planthopper Dao Qu that moves into, so its field initial population is very low, often just plays a significant role to the late rice middle and later periods.And its population propagation limits by food again, and when foodstuff and subalimentation, its fertility is severely limited.Solve the food problems of black porch green plant bug, have important use to be worth for improving the control potential of black porch green plant bug to planthopper.
Begin to carry out the artificial feeding of fleahopper class insect both at home and abroad the eighties in last century.Pollen can make short and small long ridge fleahopper Macrolophus pygmaeus(Bjorn and Patrick, 2010) finish from generation to generation and grow, but egg laying amount significantly reduces than nature food.Mediterranean flour moth (Ephestia kuehniella) ovum is used in raises black dull long ridge fleahopper (Macrolophus caliginosus) (Cristina and Rafael, 2005; Bjorn et al., 2006), Ta Mani hunts fleahopper (Dicyphus tamaninii) (Rafael et al., 2005), short and small long ridge fleahopper (Macrolophus pygmaeus) (Bjorn and Patrick, 2010; Barbara L.et al., 2011), rice moth egg is used to raise black porch green plant bug (Cyrtorhinus lividipennis) (Liu Jiali etc., 2009), but all is time-consuming, or feeding effect is not very good.
For omnivorousness fleahopper class, the exploration of rearing method and the research work of man-made feeds both at home and abroad mainly concentrates on Chinese aculea fleahopper Campylomma chinensis and cigarette fleahopper Nesidiocoris tenuis.And be in space state about the research field of black porch green plant bug man-made feeds always.Thereby development is suitable for the man-made feeds prescription of black porch green plant bug nymph and adult, carries out a large amount of artificial feedings the laboratory in, and the worm of guarantee test usefulness worm is consistent with nutrition condition age, and the man-made feeds of developing a kind of suitable black porch green plant bug are very necessary.
The research of black porch green plant bug man-made feeds and artificial propagation is conducive to all many-sides such as scientific research, biological control.
Summary of the invention
On the one hand, the object of the present invention is to provide a kind of raising predator insect-black porch green plant bug adult and nymph man-made feeds.
The man-made feeds of black porch green plant bug adult provided by the invention and nymph, by comprising that following materials based on weight makes:
Tussah chrysalis homogenate 20-40 part; Egg yolk 7-21 part; Whole milk powder 1-4 part; Sucrose 3-8 part; Lecithin 0.8-1.5 part; Choline Chloride 0.05-0.12 part; Beer yeast powder 0.2-0.4 part; Rice ketone 0.1-0.3 part; Vitamin C 0.1-0.3 part; Vitamin B compound 0.02-0.05 part; Nipalgin 0.001-0.004 part; Water 30-50 part.
The described raw material of making man-made feeds most preferably is made up of the component of following portions by weight:
30 parts of tussah chrysalis homogenates; 14 parts of egg yolks; 2.6 parts of whole milk powders; 5 parts of sucrose; 1.5 parts in lecithin; 0.2 part of rice ketone; 0.08 part of Choline Chloride; 0.3 part of beer yeast powder; 0.2 part of vitamin C; 0.04 part of vitamin B compound; 0.002 part of pool gold; 40 parts in water.
On the other hand, the invention provides a kind of method of preparing above-mentioned black porch green plant bug adult and nymph man-made feeds, said method comprising the steps of: the preparation of (1) tussah chrysalis homogenate: fresh tussah chrysalis is removed dead silkworm, impurity, wash repeatedly with running water, be neutral until cleaning solution pH value, water-bath was taken out in 30 minutes in 60 ℃ of water-baths, cut open along the outside of belly and to remove midgut, take out hemolymph, put into refiner and carry out homogenate, obtain the silkworm chrysalis homogenate; (2) fresh hen egg shells, goes egg white, gets yolk in the yolk peplos, puts into the refiner stirring and shakes up, and the egg yolk that takes by weighing described amount then places the beaker of step (1); (3) whole milk powder adds water and is made into 10% concentration, and sterilization in 10 minutes is boiled in heating, measures the solution that described deal milk power solution places step (2) to make; (4) take by weighing sucrose, lecithin, Choline Chloride, beer yeast powder, rice ketone, vitamin C, vitamin B compound, nipalgin respectively, and each solid constituent that will take by weighing is dissolved in the beaker that described deal sterilized water is housed, mix, join the prepared mixed liquor of step (3), stir, namely obtain described feed;
The present invention also provides a kind of method of using above-mentioned black porch green plant bug adult and nymph man-made feeds to raise the black porch green plant bug, said method comprising the steps of: (1) inserts adult and the nymph of black porch green plant bug at the vessel of putting into above-mentioned black porch green plant bug man-made feeds; (2) relative humidity 80-90%, the photoperiod is L:D=14:10; (3) changed a feed every 3-5 days.In preferred scheme, described black porch green plant bug man-made feeds are with sealing the film parcel, and in preferred scheme, the described film that seals is that Parafilm seals film.
Beneficial effect
Man-made feeds provided by the present invention can be used for raising predator insect black porch green plant bug, are suitable for adult and the nymph of black porch green plant bug, also are applicable to carnivorous, the omnivorousness stinkbug class insect of other kinds.Man-made feeds of the present invention are used in evidence, the code test that can obtain worm age and physiological situation unanimity with the black porch green plant bug become, nymph; It is a kind of method for breeding of producing the black porch green plant bug in batches; The black porch green plant bug that the black porch green plant bug of raising with man-made feeds of the present invention is raised than rice moth egg, nymph go through the phase to lack, the nymph survival rate significantly improves, adult life-span significant prolongation, fertility significantly improve, simultaneously, with the black porch green plant bug groupy phase ratio that the brown paddy plant hopper ovum is raised, above-mentioned biological parameter is close.
Man-made feeds composition of the present invention source is wide, easily buys, and less investment, production cost is low, and no season limit is suitable for using in the industry popularization.
The specific embodiment
Those skilled in the art will the present invention be described in further detail in the mode of embodiment below, so that can put into practice the present invention.Should be appreciated that and to adopt other embodiments, and can make suitable change and without departing from the spirit and scope of the present invention.For fear of for making those skilled in the art can put into practice unnecessary details the present invention, specification may omit some known to those skilled in the art information.Therefore, below describe in detail and should not understand with restrictive meaning, and scope of the present invention is only defined by claims.
Employed each raw material among the following embodiment, except particularly pointing out, all can be by commercially available acquisition.
Experimental technique among the following embodiment if no special instructions, is conventional method.
Percentage composition among the following embodiment if no special instructions, is the quality percentage composition.
The preparation of embodiment 1, man-made feeds
The man-made feeds of present embodiment, made by the raw material of following portions by weight ratio:
30 parts of tussah chrysalis homogenates; 14 parts of egg yolks.
Earlier fresh tussah chrysalis is rinsed well with running water, water-bath was taken out in 30 minutes in 60 ℃ of water-baths, cut open along the outside of belly and removed midgut, took out hemolymph, put into refiner and carried out homogenate, and it is standby to obtain the silkworm chrysalis homogenate.Get fresh hen egg and shell, go egg white, get yolk in the yolk peplos.Take by weighing tussah chrysalis homogenate and the egg yolk of above-mentioned ratio of weight and number, stir with agitator, to seal the film sealing, put into 4 ℃ of preservations of refrigerator, preparation is available week age once.
The preparation of embodiment 2, man-made feeds
The man-made feeds of present embodiment, made by the raw material of following portions by weight ratio:
25 parts of tussah chrysalis homogenates; 17 parts of egg yolks; 2.6 parts of whole milk powders; 5 parts of sucrose; 1.5 parts in lecithin.
Earlier fresh tussah chrysalis is rinsed well with running water, water-bath was taken out in 30 minutes in 60 ℃ of water-baths, cut open along the outside of belly and removed midgut, took out hemolymph, put into refiner and carried out homogenate, and it is standby to obtain the silkworm chrysalis homogenate.Get fresh hen egg and shell, go egg white, get yolk in the yolk peplos.Whole milk powder adds water and is made into 10% concentration, and sterilization in 10 minutes is boiled in heating.Stir and evenly mix than with tussah chrysalis homogenate, egg yolk, whole milk powder solution glass bar according to above-mentioned deal, add sucrose then, lecithin stirs with agitator, to seal the film sealing, put into 4 ℃ of preservations of refrigerator, preparation is available week age once.
The preparation of embodiment 3, man-made feeds
The man-made feeds of present embodiment, made by the raw material of following portions by weight ratio:
37.5 parts of tussah chrysalis homogenates; 7.5 parts of egg yolks; 3.75 parts of whole milk powders; 6 parts of sucrose; 1.0 parts in lecithin; 0.25 part of rice ketone; 0.05 part of Choline Chloride; 0.25 part of beer yeast powder; 0.25 part of vitamin C; 0.04 part of vitamin B compound; 0.002 part of nipalgin; 40 parts in water.
The mixture that above-mentioned vitamin B compound is made up of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, the ratio of weight and number of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 are 1:3:1:3:40:5.
Earlier fresh tussah chrysalis is rinsed well with running water, water-bath was taken out in 30 minutes in 60 ℃ of water-baths, cut open along the outside of belly and removed midgut, took out hemolymph, put into refiner and carried out homogenate, and it is standby to obtain the silkworm chrysalis homogenate.Get fresh hen egg and shell, go egg white, get yolk in the yolk peplos.Whole milk powder adds water and is made into 10% concentration, and sterilization in 10 minutes is boiled in heating.Stir and evenly mix than with tussah chrysalis homogenate, egg yolk, whole milk powder solution glass bar according to above-mentioned deal, add sucrose, lecithin, rice ketone, Choline Chloride, beer yeast powder, vitamin C, vitamin B compound and nipalgin then, stir with agitator, to seal the film sealing, put into 4 ℃ of preservations of refrigerator, preparation is available week age once.
The preparation of embodiment 4, man-made feeds
The man-made feeds of present embodiment, made by the raw material of following portions by weight ratio:
28 parts of tussah chrysalis homogenates; 10 parts of egg yolks; 2.5 parts of whole milk powders; 6 parts of sucrose; 0.2 part of 1.2 portions of rice of lecithin ketone; 0.05 part of Choline Chloride; 0.25 part of beer yeast powder; 0.25 part of vitamin C; 0.03 part of vitamin B compound; 0.002 part of nipalgin; 30 parts in water.
The mixture that above-mentioned vitamin B compound is made up of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, the ratio of weight and number of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 are 1:3:1:3:40:5.
Earlier fresh tussah chrysalis is rinsed well with running water, water-bath was taken out in 30 minutes in 60 ℃ of water-baths, cut open along the outside of belly and removed midgut, took out hemolymph, put into refiner and carried out homogenate, and it is standby to obtain the silkworm chrysalis homogenate.Get fresh hen egg and shell, go egg white, get yolk in the yolk peplos.Whole milk powder adds water and is made into 10% concentration, and sterilization in 10 minutes is boiled in heating.Stir and evenly mix than with tussah chrysalis homogenate, egg yolk, whole milk powder solution glass bar according to above-mentioned deal, add sucrose, lecithin, rice ketone, Choline Chloride, beer yeast powder, vitamin C, vitamin B compound and nipalgin then, stir with agitator, to seal the film sealing, put into 4 ℃ of preservations of refrigerator, preparation is available week age once.
The preparation of embodiment 5, man-made feeds
The man-made feeds of present embodiment, made by the raw material of following portions by weight ratio:
30 parts of tussah chrysalis homogenates; 14 parts of egg yolks; 2.6 parts of whole milk powders; 5 parts of sucrose; 1.5 parts in lecithin; 0.2 part of rice ketone; 0.08 part of Choline Chloride; 0.3 part of beer yeast powder; 0.2 part of vitamin C; 0.04 part of vitamin B compound; 0.002 part of nipalgin; 40 parts in water.
The mixture that above-mentioned vitamin B compound is made up of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, the ratio of weight and number of folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 are 1:3:1:3:40:5.
Earlier fresh tussah chrysalis is rinsed well with running water, water-bath was taken out in 30 minutes in 60 ℃ of water-baths, cut open along the outside of belly and removed midgut, took out hemolymph, put into refiner and carried out homogenate, and it is standby to obtain the silkworm chrysalis homogenate.Get fresh hen egg and shell, go egg white, get yolk in the yolk peplos.Whole milk powder adds water and is made into 10% concentration, and sterilization in 10 minutes is boiled in heating.Stir and evenly mix than with tussah chrysalis homogenate, egg yolk, whole milk powder solution glass bar according to above-mentioned deal, add sucrose, lecithin, rice ketone, Choline Chloride, beer yeast powder, vitamin C, vitamin B compound and nipalgin then, stir with agitator, to seal the film sealing, put into 4 ℃ of preservations of refrigerator, preparation is available week age once.
It will be appreciated that each step of described compound method is not limited to above order, carry out each step in proper order with other and also can realize the present invention.
The feeding effect experiment of embodiment 6 feeds of the present invention
The black porch green plant bug of feeding is used, seal the man-made feeds parcel that film (all there is sale in biological chemical reagent company) will prepare in right amount with Parafilm, and with the fine rule wrapping, guaranteeing that feed does not spill, the one-tenth of black porch green plant bug, nymph penetrate film by sucking mouth parts and get anthropophagy worker's feed.With fresh rice moth egg with produce brown paddy plant hopper ovum feed in contrast on the paddy rice seedling.
In plastics crisper (long 20cm, high 15cm, high 8cm), put into Parafilm respectively and seal the prepared prescription feed of the present invention of an amount of above-described embodiment and the control feed that film wraps, every box inserts the black porch green plant bug just incubates 100 of nymphs, every kind of feed connects 3 boxes, namely carries out 3 repetitions.The plastics crisper that inserts nymph is covered with four layers of hospital gauze, and restraint and go up rubber band, prevent the nymph escape, then the plastics crisper is placed illumination box, temperature is 26 ℃ ± 1 ℃, relative humidity 80%-90%, and the photoperiod is L:D=14:10.Record black porch green plant bug nymph survival condition and development progress every day.Changed a feed in per 3 days.
Just incubate nymph with above all feeds in above-mentioned condition raising, up to emergence.The adult pairing of just having sprouted wings is inserted the teat glass that is placed with fresh water rice seedling (oviposition substrate), place the man-made feeds of Parafilm film parcel in the test tube, every kind of feed repeats 30 times.Changed fresh rice seedling in per 3 days, record ovum amount is dissected, checked to the rice seedling that changes under stereomicroscope, all dead until all female worms.
Table 1 get anthropophagy worker's feed and rice moth egg, the nymph survival rate (%) of brown paddy plant hopper ovum, nymphal development go through the phase (my god), adult life-span sky (my god) and egg laying amount (grain)
Figure BDA00003290098800061
The result shows, the black porch green plant bug is on the feed of man-made feeds formulated of the present invention, survival rate height, adult life-span be long, grow fast, egg laying amount is high, These parameters all close to the black porch green plant bug of raising with natural feed brown paddy plant hopper ovum, is significantly higher than the black porch green plant bug of raising with the natural feed rice moth egg.Illustrate that black porch green plant bug man-made feeds of the present invention are suitable for the mass rearing of black porch green plant bug nymph and adult.Wherein best with the feeding effect of embodiment 5 again.Black porch green plant bug man-made feeds of the present invention have excellent economy and experimental.
In sum; being preferred embodiment of the present invention only below, is not for restriction protection scope of the present invention, therefore; all any modifications of doing within the spirit and principles in the present invention, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (10)

1. the man-made feeds of a black porch green plant bug adult or nymph comprise following materials based on weight: tussah chrysalis homogenate 20-40 part; Egg yolk 7-21 part.
2. black porch green plant bug adult according to claim 1 or nymph man-made feeds is characterized in that, also comprise following materials based on weight in the described raw material of making man-made feeds: whole milk powder 1-4 part; Sucrose 3-8 part; Lecithin 0.8-1.5 part.
3. a black porch green plant bug adult or nymph man-made feeds, it is characterized in that: the described raw material of making man-made feeds is made up of the component of following portions by weight: tussah chrysalis homogenate 20-40 part; Egg yolk 7-21 part; Whole milk powder 1-4 part; Sucrose 3-8 part; Lecithin 0.8-1.5 part; Choline Chloride 0.05-0.12 part; Beer yeast powder 0.2-0.4 part; Rice ketone 0.1-0.3 part; Vitamin C 0.1-0.3 part; Vitamin B compound 0.02-0.05 part; Nipalgin 0.001-0.004 part; Water 30-50 part.
4. black porch green plant bug adult according to claim 3 or nymph man-made feeds, it is characterized in that: the described raw material of making man-made feeds is made up of the component of following portions by weight: 30 parts of tussah chrysalis homogenates; 14 parts of egg yolks; 2.6 parts of whole milk powders; 5 parts of sucrose; 1.5 parts in lecithin; 0.2 part of rice ketone; 0.08 part of Choline Chloride; 0.3 part of beer yeast powder; 0.2 part of vitamin C; 0.04 part of vitamin B compound; 0.002 part of nipalgin; 40 parts in water.
5. according to any described black porch green plant bug adult or nymph man-made feeds among the claim 1-4, it is characterized in that described vitamin B compound is folic acid, pantothenic acid, VB1, VB2, VB3 or VB12.
6. black porch green plant bug adult according to claim 5 or nymph man-made feeds, it is characterized in that: described vitamin B compound is by folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, and the ratio of weight and number of described folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 is 1:3:1:3:40:5.
7. according to any described black porch green plant bug adult or nymph man-made feeds among the claim 1-7, wherein, collocation method may further comprise the steps:
The preparation of tussah chrysalis homogenate: fresh tussah chrysalis is removed dead silkworm, impurity, wash repeatedly with running water, be neutral until cleaning solution pH value, water-bath was taken out in 30 minutes in 60 ℃ of water-baths, cut open along the outside of belly and to remove midgut, take out hemolymph, put into refiner and carry out homogenate, obtain the silkworm chrysalis homogenate;
Fresh hen egg shells, goes egg white, gets yolk in the yolk peplos, puts into the refiner stirring and shakes up, and the egg yolk that takes by weighing described amount then places the beaker of step (1);
Whole milk powder adds water and is made into 10% concentration, and sterilization in 10 minutes is boiled in heating, measures the solution that described deal milk power solution places step (2) to make;
Take by weighing sucrose, lecithin, Choline Chloride, beer yeast powder, rice ketone, vitamin C, vitamin B compound, nipalgin respectively, and each solid constituent that will take by weighing is dissolved in the beaker that described deal sterilized water is housed, mix, join the prepared mixed liquor of step (3), stir, namely obtain described feed.
8. the application of any described man-made feeds in raising black porch green plant bug insect among the claim 1-7.
9. according to right 8 described application, it is characterized in that: the described raw material of making man-made feeds is made up of the component of following portions by weight: 30 parts of tussah chrysalis homogenates; 14 parts of egg yolks; 2.6 parts of whole milk powders; 5 parts of sucrose; 1.5 parts in lecithin; 0.2 part of rice ketone; 0.08 part of Choline Chloride; 0.3 part of beer yeast powder; 0.2 part of vitamin C; 0.04 part of vitamin B compound; 0.002 part of nipalgin; 40 parts in water.
10. according to right 8 described application, it is characterized in that: described vitamin B compound is by folic acid, pantothenic acid, VB1, VB2, VB3 and VB12, and the ratio of weight and number of described folic acid, pantothenic acid, VB1, VB2, VB3 and VB12 is 1:3:1:3:40:5.
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CN103609529A (en) * 2013-09-18 2014-03-05 中国农业科学院植物保护研究所 Method for evaluating influences of biological protein on apolygus lucorum
CN104351503A (en) * 2014-10-16 2015-02-18 中国农业大学 Artificial feeds for heteroptera:Miridae
CN104351503B (en) * 2014-10-16 2019-01-29 中国农业大学 A kind of green plant bug man-made feeds
CN104304682A (en) * 2014-11-03 2015-01-28 杨征 Nutrient solution for artificially culturing golden cicada with effect of nourishing qi and blood
CN105851720A (en) * 2016-04-27 2016-08-17 浙江省农业科学院 Artificial feed for tytthus chinensis
CN107927356A (en) * 2017-12-06 2018-04-20 中国农业科学院植物保护研究所 A kind of gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation
CN108402343A (en) * 2018-04-09 2018-08-17 华南农业大学 A kind of man-made feeds formula of Predacious function
CN108402343B (en) * 2018-04-09 2022-03-22 华南农业大学 Artificial feed formula for harris baccarum
CN109329608A (en) * 2018-10-29 2019-02-15 扬州大学 A kind of man-made feeds and its preparation method and application for Mirid, cyrtorhinus
CN109329608B (en) * 2018-10-29 2022-03-29 扬州大学 Artificial feed for lygus lucorum, and preparation method and application thereof
CN109757441A (en) * 2019-03-13 2019-05-17 中国农业科学院植物保护研究所 A method of storage Candle-sticks stinkbug
CN115956642A (en) * 2022-11-30 2023-04-14 贵州省烟草科学研究院 Armoracia chinensis artificial feed and preparation method thereof

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