CN101427666A - Artificial breeding method for leek maggot for test - Google Patents
Artificial breeding method for leek maggot for test Download PDFInfo
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- CN101427666A CN101427666A CNA2008102387136A CN200810238713A CN101427666A CN 101427666 A CN101427666 A CN 101427666A CN A2008102387136 A CNA2008102387136 A CN A2008102387136A CN 200810238713 A CN200810238713 A CN 200810238713A CN 101427666 A CN101427666 A CN 101427666A
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Abstract
The invention relates to an artificial feeding method of tested insect, especially relates to an artificial feeding method of tested chive maggot, wherein the Chinese chive feedstuff is replaced by the artificial synthetic feedstuff, thus the survival rate, pupation rate, emergence rate, single-female fecundity are greatly increased compared with the prior tested chive maggot breeding method, therefore the good condition is provided for regular supply of tested chive maggot.
Description
Technical field
The present invention relates to a kind of rearing method, particularly a kind of rearing method for examination fragrant-flowered garlic maggot for the examination insect.
Background technology
Fragrant-flowered garlic maggot---leek eye fungus gnat Bradysia odoriphay late is the No.1 insect of leek, directly threatens and restricting safety in production and the sale of leek.For a long time, the vegetable grower mainly relies on high poison or hypertoxic insecticide, as parathion-methyl, and thimet (3911), acephatemet, the different sulphur phosphorus of methyl, this obstinate insect of control such as furans pellet, even arbitrarily strengthen dose, cause urban and rural residents' poisoning or death by accident often to take place.The Shandong Province is in recent years in Dezhou, diet repeatedly takes place and causes poisoning with leek in Boxing, ground such as Ningjin, wherein the most serious cause 70 surplus people's acute poisoning, the residual hazard problem of leek has caused social worried and concern, has become problem anxious to be solved in the vegetables.
Along with people to the concern of the general level of the health and raising that quality of life is required, in the pollution-free food pollution-free vegetable produce and the cry of agricultural sustainable development more and more stronger.Basic conception and the specification requirement that pollution-free vegetable produces be " for guaranteeing certain output and benefit, production environment is not produced pollute and the agricultural product body under the prerequisite that do not exceed standard of poisonous residuals, allow to limit the quantity of and use the chemical substance of qualification." that is to say that pollution-free vegetable produces, allow to limit the quantity of and use some chemical fertilizer, low-toxin farm chemicals etc., but the poisonous residuals in the vegetables can not surpass state-set standard.Advance most, Beijing, special requirement and regulation have all been done to the nuisanceless production of leek in big cities such as Shanghai.
Large scale artificial breeding's technology for the examination insect is one of important means of modern control of insect research.The fragrant-flowered garlic maggot is as research and controlling object; especially carry out biological control and pesticide resistance and measure research work such as steriliation by irradiation; study its synthetic feed and mass rearing propagation technique; solve examination worm supply problem; to the nuisanceless production of leek is crucial; existing fragrant-flowered garlic maggot breeding technology is generally traditional fragrant-flowered garlic maggot raising leek section; be that leek gathers; remove impurity; peeling; with the cultivated 4-6mm segment that is cut into of leek; edible for fragrant-flowered garlic maggot larva, all adopt natural leek as feed in whole feeding process, can't be removed completely owing to all have certain residue of pesticide in the existing leek; so just make the raising safety of fragrant-flowered garlic maggot can not get guaranteeing; murder by poisoning for the fragrant-flowered garlic maggot is bigger, causes the discontinuous of raising through regular meeting, makes many generations of fragrant-flowered garlic maggot raise and can't carry out; so just can't guarantee uniformity for the examination insect; reduced the confidence level and the representativeness of result of the test, and the fragrant-flowered garlic maggot susceptibility of gained is poor, result of the test is inaccurate.
Summary of the invention
At existing many deficiencies for the existence of examination fragrant-flowered garlic maggot raising method, the invention provides a kind of rearing method for examination fragrant-flowered garlic maggot, feed with synthetic has replaced the existing omnidistance raising method that uses leek as feed, after cooperating raising method of the present invention simultaneously, than existing its survival rate of examination fragrant-flowered garlic maggot raising method, pupation rate, the eclosion rate of supplying, single female egg laying amount is enhanced, for the regular supply for examination fragrant-flowered garlic maggot provides strong condition.
Rearing method for examination fragrant-flowered garlic maggot of the present invention mainly comprises the steps:
(1) selecting diameter for use is the culture dish of 5-12cm, puts the filter paper of the smaller culture dish of a diameter within it, drips on filter paper, makes filter paper moistening;
(2) with the ovum that is produced in the same time period of fragrant-flowered garlic maggot adult, forward in the culture dish, hatch small grub certainly after, feed with the synthetic diet section, and in time remove swill and excreta;
(3) raise after 23-26 days, larva is near when aging, puts a filter paper in culture dish again, and its area is less than the filter paper described in the step 1, and larva climbs to spinning between two layers of filter paper and pupates to obtain adult;
(4) will go up step gained adult and forward in the clean container, the bottom of container is covered with moistening filter paper, makes adult carry out mating, after 12-24 hour, is that the natural leek section of 0.3-0.7cm is placed on the filter paper in the container with 2-4 length, and adult is laid eggs thereon.
Above-mentioned feeding process is 25-28 ℃ in temperature, relative moisture is to carry out in the thermostatic chamber of 75-85%, because larva grows in the process of adult, temperature and relative moisture have decisive role, slowly grow and cause the physique difference of adult in order to prevent the too fast of larva or to cross, temperature and relative moisture are controlled in the above-mentioned scope.
Be placed with nutriment on the filter paper in described step (4), this nutriment generally can adopt the little silk tops that has nutrient solution, and used nutrient solution is hydromel or syrup or other nutrient solutions.The sustainable supply of nutrition in the time of can guaranteeing the adult mating like this improves the efficient and the quality of breeding, why adopts silk tops can prevent that as carrier nutrient solution from too much causing adult by drowned; Can recycle simultaneously, reduce the cost of raising.
Clean container in the described step (4) is transparent plastic casing, generally can adopt the jelly cup that passes through after sterilizing as container, has so both guaranteed the required space of adult mate and oviposit, has made things convenient for experimenter's observation again.
Though existing raising method has similar place to method provided by the present invention, but because it adopts all are natural leek as the feed of raising, the environmental condition of raising is had relatively high expectations, and because shortcoming such as natural leek itself is perishable makes existing raising method exist bigger defective; Compare with existing disclosed raising method, though method of the present invention has similarity in form, but owing to adopted artificial feed, raising method that the present invention who is is used and existing method have had substantial difference, after adopting artificial feed, the time of guaranteeing the quality of feed increases, the ability of high-temp resisting high-humidity resisting is strengthened, therefore can it be 25-28 ℃ in temperature just with feeding process, relative moisture is to carry out in the thermostatic chamber of 75-85%, because the increase of temperature humidity has improved the growth rate of fragrant-flowered garlic maggot, the confession examination fragrant-flowered garlic maggot of turning out qualified health that compared to prior art can be faster and better.
Feed section in the described step (2) is the long synthetic mushroom feed section of 0.3-0.7cm.These artificial feed are according to the weight portion meter: mushroom powder 150-165 part, and the proportioning processing of dusty yeast 55-65 part makes, and preferably adopts 155-157 part, and the amount of selecting dusty yeast simultaneously for use is 60-63 part; Because the main nutrient composition that wherein contains is close with the nutrient component of leek, also contains nutriments such as amounts of protein simultaneously, can replace leek as the main feed in the feeding process fully.Because this synthetic diet is made by mushroom powder, do not have the residual of agricultural chemicals, so just guaranteed the safety in the feeding process, simultaneously owing to the dependence that has reduced for natural leek, making can not stop production owing to the seasonality of leek in feeding process causes the anxiety of feed, has realized the serialization raising; Simultaneously since the mushroom powder synthetic diet than natural leek, the homogeneity of its quality and stability have great raising, so just guaranteed the uniformity of the fragrant-flowered garlic maggot quality of being raised out, can not cause the big rise and big fall of fragrant-flowered garlic maggot quality owing to the relation of feed, for Bioexperiment, this uniformity is of crucial importance; Because the stimulation component that is contained in the mushroom powder synthetic diet is lower, can be with regard to each that has guaranteed to breed owing to stimulation component such as agricultural chemicals develop immunity to drugs for the fragrant-flowered garlic maggot, improved the susceptibility of fragrant-flowered garlic maggot, thereby improved the accuracy rate and the authenticity of Bioexperiment for test.
Except above-mentioned major ingredient, also be added with in this synthetic diet: preservative 4-6 part, agar 20-30 part.Add man-hour, concrete steps are as follows:
(1) with mushroom powder, dusty yeast is mixing in proportion, adds the water of 2-3 times of weight, stirs;
(2) agar powder is proportionally joined in the water of 15-25 times of weight, after the heating for dissolving, proportionally add preservative again, stir, make agar solution;
(3) agar solution that makes in (2) is poured in the mixed liquor that makes in (1), stirred, it is standby that feed is made in cooling.
Before raising beginning, need feeding environment is carried out disinfection, for the culture dish of breeding grub, tweezers, cutter etc., bromogeramine can be made thimerosal for 15 times with the sterile distilled water dilution, afterwards used utensil is put into thimerosal, soaked 24 hours, and washed down with running water again and can use after sky is done.
Filter paper described in the step 1 is being carried out when wetting, needing the control water consumption, preventing that the minute surface reflection from appearring in the filter paper surface, add dilutional hyponatremia when the explanation of minute surface reflex time occurring, larva is easily drowned in culture dish, reduces final survival rate and yield, and generally controlling its amount of water is that 9-13 drips.
Because temperature and the relative moisture that will raise have been controlled in the above-mentioned scope, larva generally will stop to get food behind 23-26 aging, and the larva filter paper fibre of can weaving silk and break by the teeth is done in wherein to pupate.Cause larva to build up to pupate easily like this, building up the inconvenience of pupating for fear of larva observes and causes death, reduce the survival rate of raising, therefore treating that larva is near when aging, put a fritter filter paper in the ware, larva can spin between two layers of filter paper and pupate like this, thereby has reduced the quantity that larva builds up, and has improved the survival rate of larva simultaneously so that observe.
Through the fragrant-flowered garlic maggot adult that above-mentioned steps is raised, can be used as using of test for the examination insect; Also can make adult continue mate and oviposit and obtain 2 generation body or more offspring's body for deliberation, when adult carries out the mating breeding, the above-mentioned step (4) of general employing is operated and is got final product, owing to also well do not lure the method that fragrant-flowered garlic maggot adult lays eggs now into, therefore adopting 2-4 length is the method that the natural leek section of 0.3-0.7cm is put into container, luring that fragrant-flowered garlic maggot adult lays eggs on natural leek section into gets final product, the avirulence that so only needs to guarantee natural leek section in this step gets final product, simultaneously since this natural leek not as the feed of larva, just can not influence continuity and the quality that the fragrant-flowered garlic maggot is raised yet, improve quality for examination fragrant-flowered garlic maggot.
In sum, adopt above-mentioned raising method after, than existing for examination fragrant-flowered garlic maggot raising method, the homogeneity of gained fragrant-flowered garlic maggot quality and stability with and survival rate, pupation rate, eclosion rate, single female egg laying amount is enhanced, for the regular supply for examination fragrant-flowered garlic maggot provides strong condition.Improved the susceptibility of fragrant-flowered garlic maggot simultaneously for test, thereby improved the accuracy rate and the authenticity of Bioexperiment, and this raising method, owing to adopted the synthetic feed not to be subject to seasonal restrictions, and be not subjected to the influence of remains of pesticide and quality in the leek, improved the continuity of raising.
Embodiment
Embodiment 1
A kind of rearing method for examination fragrant-flowered garlic maggot mainly comprises the steps:
(1) selecting diameter for use is the culture dish of 8cm, puts the filter paper that a diameter is 7cm within it, and the 10-12 that drips on filter paper drips, and makes filter paper moistening;
(2) with the ovum that is produced in the same time period of fragrant-flowered garlic maggot adult, forward in the culture dish, hatch small grub certainly after, feed with the synthetic diet section, and in time remove swill and excreta;
(3) raise after 24-25 days, larva is near when aging, puts a filter paper in culture dish, and its diameter is 6.5cm, and larva climbs to spinning between two layers of filter paper and pupates to obtain adult;
(4) will go up step gained adult forwards in the clean transparent jelly cup, the bottom of jelly cup is covered with moistening filter paper, be placed with the little silk tops of a band hydromel on the filter paper, make adult on filter paper, carry out mating, after 20-24 hour, with 2 length is that the natural leek section of 0.3-0.5cm is placed on the filter paper in the container, and adult is laid eggs thereon.
Described feeding process is 28 ℃ in temperature, and relative moisture is to carry out in 80% the thermostatic chamber; The feed section that adopts in the step (2) is the long synthetic mushroom feed section of 0.3-0.5cm, and its proportioning is by weight: 8 parts of mushroom powders, 2.81 parts of dusty yeasts, 1.45 parts of agar powders, 0.06 part in benzoic acid, 0.18 part of Sodium Benzoate, 0.05 part of sorbic acid.
The concrete compound method of feed is:
(1) with mushroom powder and dusty yeast according to aforementioned proportion, add in 31 parts of water and stir;
(2) agar powder with aforementioned proportion adds in 36 parts of water, after the heating for dissolving, adds benzoic acid again in micro-wave oven, and Sodium Benzoate and sorbic acid stir, and make agar solution;
(3) with agar solution to going in the mixed liquor that makes in (1), stir, to make feed standby in cooling.
Embodiment 2
A kind of rearing method for examination fragrant-flowered garlic maggot mainly comprises the steps:
(1) selecting diameter for use is the culture dish of 12cm, puts the filter paper that a diameter is 10cm within it, and the 11-13 that drips on filter paper drips, and makes filter paper moistening;
(2) with the ovum that is produced in the same time period of fragrant-flowered garlic maggot adult, forward in the culture dish, hatch small grub certainly after, feed with the synthetic diet section, and in time remove swill and excreta;
(3) raise after 23-25 days, larva is near when aging, puts a filter paper in culture dish, and its diameter is 9cm, and larva climbs to spinning between two layers of filter paper and pupates to obtain adult;
(4) will go up step gained adult forwards in the clean transparent jelly cup, the bottom of jelly cup is covered with moistening filter paper, be placed with the little silk tops of a band hydromel on the filter paper, make adult on filter paper, carry out mating, after 18-20 hour, with 4 length is that the natural leek section of 0.5-0.7cm is placed on the filter paper in the container, and adult is laid eggs thereon.
Described feeding process is 27 ℃ in temperature, and relative moisture is to carry out in 85% the thermostatic chamber; The feed section that adopts in the step (2) is the long synthetic mushroom feed section of 0.5-0.7cm, and its proportioning is: 9 parts of mushroom powders, 3.36 parts of dusty yeasts, 1.25 parts of agar powders, 0.05 part in benzoic acid, 0.15 part of Sodium Benzoate, 0.25 part of honey, 0.04 part of sorbic acid.
The concrete compound method of feed is:
(1) with mushroom powder, dusty yeast is according to aforementioned proportion, adds in 35 parts of water to stir;
(2) agar powder with aforementioned proportion adds in 25 parts of water, after the heating for dissolving, adds benzoic acid again, Sodium Benzoate, and sorbic acid and honey stir, and make agar solution;
(3) with agar solution to going in the mixed liquor that makes in (1), stir, to make feed standby in cooling.
Embodiment 3
A kind of rearing method for examination fragrant-flowered garlic maggot mainly comprises the steps:
(1) selecting diameter for use is the culture dish of 5cm, puts the filter paper that a diameter is 4.5cm within it, and the 9-10 that drips on filter paper drips, and makes filter paper moistening;
(2) with the ovum that is produced in the same time period of fragrant-flowered garlic maggot adult, forward in the culture dish, hatch small grub certainly after, feed with the synthetic diet section, and in time remove swill and excreta;
(3) raise after 24-26 days, larva is near when aging, puts a filter paper in culture dish, and its diameter is 4cm, and larva climbs to spinning between two layers of filter paper and pupates to obtain adult;
(4) will go up step gained adult forwards in the clean transparent plastic cup, the bottom of plastic cup is covered with moistening filter paper, be placed with the little silk tops of a band syrup on the filter paper, make adult on filter paper, carry out mating, after 22-24 hour, with 3 length is that the natural leek section of 0.3-0.5cm is placed on the filter paper in the container, and adult is laid eggs thereon.
Described feeding process is 25 ℃ in temperature, and relative moisture is to carry out in 75% the thermostatic chamber; The feed section that adopts in the step (2) is the long synthetic mushroom feed section of 0.3-0.5cm, and its proportioning is: 9 parts of mushroom powders, 3.36 parts of dusty yeasts.
The concrete compound method of feed is:
With mushroom powder, dusty yeast is according to aforementioned proportion, adds in 24 parts of water to stir; It is standby that feed is made in cooling.
Comparative example:
Under identical condition, adopt natural feed (leek) to feed the fragrant-flowered garlic maggot as the conventional raising method and the embodiment 1 described method of feed, each is handled and repeats 10 times.Connect 200～300 ovum in each culture dish, guarantee that the interior larva number of each culture dish is greater than 100.Every day in pupa time, 24h divided three periods to observe, and in time collected pupa, performed record, every kind of processing is repeated resulting pupa all differentiate male and female, divided another name male and female pupa heavy.Then with the every kind of pupa that arrives mixing of handling to repeat institute for 10 times, therefrom get 100 pairs of male and female pupas or 200 pairs at random, each is that the culture dish of 3cm is laid eggs to putting into diameter, culture dish is spread moistening filter paper, adds the cotton pellet of being with hydromel.Calculate the survival rate of fragrant-flowered garlic maggot, pupation rate, eclosion rate, the heavy and single female egg laying amount of pupa.The results are shown in following table
Table is raised the effect of fragrant-flowered garlic maggot
By comparative example as can be seen: the fragrant-flowered garlic maggot of the raising method gained that employing embodiment 1 is adopted, survival rate, pupation rate, eclosion rate, single female egg laying amount, all very approaching and all have and exceed with the fragrant-flowered garlic maggot of adopting natural feed (leek) to feed as the conventional raising method of feed, also all be higher than the fragrant-flowered garlic maggot that natural feed is fed and the average pupa of male and female is heavy.But since natural feed to be subjected to the influence in season very big, therefore existing raising method can't use for a long time, but not fresh leek is because its loss of nutrient substances, its effect has bigger gap than synthetic feed of the present invention; All contain residue of pesticide in the simultaneously fresh leek feed, safety is much lower than raising method of the present invention in use.
Claims (5)
1. the rearing method for examination fragrant-flowered garlic maggot is characterized in that: mainly comprise the steps:
(1) selecting diameter for use is the culture dish of 5-12cm, puts the filter paper of the smaller culture dish of a diameter within it, drips on filter paper, makes filter paper moistening;
(2) with the ovum that is produced in the same time period of fragrant-flowered garlic maggot adult, forward in the culture dish, hatch small grub certainly after, feed with the synthetic diet section, and in time remove swill and excreta;
(3) raise after 23-26 days, larva is near when aging, puts a filter paper in culture dish again, and its area is less than the filter paper described in the step 1, and larva climbs to spinning between two layers of filter paper and pupates to obtain adult;
(4) will go up step gained adult and forward in the clean container, the bottom of container is covered with moistening filter paper, makes adult carry out mating, after 12-24 hour, is that the natural leek section of 0.3-0.7cm is placed on the filter paper in the container with 2-4 length, and adult is laid eggs thereon.
2. raising method according to claim 1 is characterized in that: described feeding process is 25-28 ℃ in temperature, and relative moisture is to carry out in the thermostatic chamber of 75-85%.
3. raising method according to claim 1 and 2 is characterized in that: be placed with nutriment on the filter paper in the described step (4).
4. raising method according to claim 1 and 2 is characterized in that: the clean container in the described step (4) is transparent plastic casing.
5. raising method according to claim 1 and 2 is characterized in that: the synthetic diet section in the described step (2) is the long synthetic mushroom feed section of 0.3-0.7cm.
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| CN2008102387136A CN101427666B (en) | 2008-12-22 | 2008-12-22 | Artificial breeding method for leek maggot for test |
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| CN101796939B (en) * | 2010-02-25 | 2012-11-28 | 浙江省农业科学院 | Egg laying device of cnaphalocrocis medinalis guenee and use method thereof |
| CN103355257A (en) * | 2013-07-15 | 2013-10-23 | 云南大学 | Breeding method and breeding box for insect larvae |
| CN104509494A (en) * | 2014-12-16 | 2015-04-15 | 山东省农业科学院植物保护研究所 | Artificial rearing method of tested Chinese chive maggot |
| CN104542498A (en) * | 2014-12-16 | 2015-04-29 | 山东省农业科学院植物保护研究所 | Artificial feeding method for tested bradysia odoriphaga |
| CN104664155A (en) * | 2015-02-10 | 2015-06-03 | 长江大学 | Culture medium for manually and rapidly feeding Chinese chive maggots and preparation method of culture medium |
| CN104824018A (en) * | 2015-05-22 | 2015-08-12 | 山东省农业科学院植物保护研究所 | Chinese chive maggot processing method |
| CN104957116A (en) * | 2015-07-08 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for luring and collecting Chinese chive maggot imagoes for laying eggs |
| CN105028361A (en) * | 2015-07-08 | 2015-11-11 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring adult Chinese chive maggots |
| CN105145488A (en) * | 2015-07-08 | 2015-12-16 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring Chinese chive maggot larvae |
| CN105918256A (en) * | 2016-03-23 | 2016-09-07 | 中国农业科学院蔬菜花卉研究所 | Simple method for artificial feeding of chinese chive maggots |
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| CN101796939B (en) * | 2010-02-25 | 2012-11-28 | 浙江省农业科学院 | Egg laying device of cnaphalocrocis medinalis guenee and use method thereof |
| CN103355257A (en) * | 2013-07-15 | 2013-10-23 | 云南大学 | Breeding method and breeding box for insect larvae |
| CN104509494A (en) * | 2014-12-16 | 2015-04-15 | 山东省农业科学院植物保护研究所 | Artificial rearing method of tested Chinese chive maggot |
| CN104542498A (en) * | 2014-12-16 | 2015-04-29 | 山东省农业科学院植物保护研究所 | Artificial feeding method for tested bradysia odoriphaga |
| CN104509494B (en) * | 2014-12-16 | 2017-01-25 | 山东省农业科学院植物保护研究所 | Artificial rearing method of tested Chinese chive maggot |
| CN104542498B (en) * | 2014-12-16 | 2016-11-30 | 山东省农业科学院植物保护研究所 | A kind of rearing method for examination fragrant-flowered garlic maggot |
| CN104664155A (en) * | 2015-02-10 | 2015-06-03 | 长江大学 | Culture medium for manually and rapidly feeding Chinese chive maggots and preparation method of culture medium |
| CN104824018A (en) * | 2015-05-22 | 2015-08-12 | 山东省农业科学院植物保护研究所 | Chinese chive maggot processing method |
| CN105145488A (en) * | 2015-07-08 | 2015-12-16 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring Chinese chive maggot larvae |
| CN105028361A (en) * | 2015-07-08 | 2015-11-11 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring adult Chinese chive maggots |
| CN104957116A (en) * | 2015-07-08 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for luring and collecting Chinese chive maggot imagoes for laying eggs |
| CN104957116B (en) * | 2015-07-08 | 2018-06-15 | 山东省农业科学院植物保护研究所 | A kind of method for trapping fragrant-flowered garlic maggot Adult worms producting eggs |
| CN105145488B (en) * | 2015-07-08 | 2019-09-06 | 山东省农业科学院植物保护研究所 | A method of it traps and monitors bradysia odoriphaga larva |
| CN105918256A (en) * | 2016-03-23 | 2016-09-07 | 中国农业科学院蔬菜花卉研究所 | Simple method for artificial feeding of chinese chive maggots |
| CN106719475A (en) * | 2016-12-13 | 2017-05-31 | 浙江省农业科学院 | The producing method for seed of Female silkworm parthenogenetic clones |
| CN108902059A (en) * | 2018-07-30 | 2018-11-30 | 岭南师范学院 | The scale hierarchical culture method of extra large cockroach |
| CN108902059B (en) * | 2018-07-30 | 2020-12-08 | 岭南师范学院 | Large-scale grading culture method for sea cockroaches |
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