CN104509494A - Artificial rearing method of tested Chinese chive maggot - Google Patents
Artificial rearing method of tested Chinese chive maggot Download PDFInfo
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- CN104509494A CN104509494A CN201410783954.4A CN201410783954A CN104509494A CN 104509494 A CN104509494 A CN 104509494A CN 201410783954 A CN201410783954 A CN 201410783954A CN 104509494 A CN104509494 A CN 104509494A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an artificial large-scale rearing method of tested insects, in particular to an artificial mass rearing method of tested Chinese chive maggot. Potatoes replace existing Chinese chive, green Chinese onion and artificial feed, and a fine sand moisturizing method replaces an agar moisturizing method and an artificial water adding moisturizing method. Compared with an existing tested Chinese chive maggot rearing method, the survival rate, the pupation rate, the emergence rate and the egg number per female of the artificial rearing method are equal to those of the existing tested Chinese chive maggot rearing method, but cost is saved, artificial operation steps are decreased, and favorable conditions are provided for normal supply of the tested Chinese chive maggot.
Description
Technical field
The present invention relates to a kind of rearing method for examination insect, particularly a kind of rearing method for examination fragrant-flowered garlic maggot.
Background technology
Fragrant-flowered garlic maggot-Bradysia odoriphaga is the No.1 insect of leek, directly threatens and govern safety in production and the sale of leek.For a long time, vegetable grower mainly relies on high poison or hypertoxic insecticide, as parathion-methyl, thimet (3911), acephatemet, the different sulphur phosphorus of methyl, this obstinate insect of the control such as Furadan, even arbitrarily strengthens dose, causes the poisoning or death by accident of urban and rural residents often to occur.Shandong Province is in recent years in Dezhou, Boxing, repeatedly there is diet and cause poisoning with leek in the ground such as Ningjin, wherein the most seriously causes more than 70 people's acute poisonings, the residual hazard problem of leek has caused worry and the concern of society, has become problem anxious to be resolved in vegetables.
Along with people are to the concern of the general level of the health and the raising to quality of life requirement, in pollution-free food, the cry of pollution-free vegetable production and agricultural sustainable development is more and more stronger.The basic conception that pollution-free vegetable produces and technical requirement are that " for ensureing certain output and benefit, under not producing to production environment the prerequisite that in pollution and agricultural product body, toxic residua matter does not exceed standard, Permissible limit uses the chemical substance limited." that is, pollution-free vegetable produces, and Permissible limit uses some chemical fertilizer, low-toxin farm chemicals etc., but the toxic residua matter in vegetables can not exceed state-set standard.Enter most, Beijing, the big cities such as Shanghai, special requirement and regulation have all been done to leek No-harmful apple orchard.
Large scale artificial breeding's technology for examination insect is one of important means of modern control of insect research.Fragrant-flowered garlic maggot is as studying and combating object, especially the research work such as biological control and pesticide resistance mensuration steriliation by irradiation is carried out, study its Prof. Du Yucang feed and mass rearing propagation technique, solve examination worm supply problem, very important to leek No-harmful apple orchard, existing fragrant-flowered garlic maggot breeding technology is generally the raising of traditional fragrant-flowered garlic maggot and uses leek section, namely leek gathers, remove impurity, peeling, leek is ploughed and is cut into 4-6mm segment, eat for fragrant-flowered garlic maggot larva, all adopt natural leek as feed in whole feeding process, owing to all cannot be removed thoroughly with certain residue of pesticide in existing leek, the raising of fragrant-flowered garlic maggot is so just made to can not be guaranteed safely, murder by poisoning for fragrant-flowered garlic maggot is larger, often can cause the discontinuous of raising, many generations of fragrant-flowered garlic maggot are made to raise and cannot carry out, so just cannot ensure the uniformity for examination insect, reduce confidence level and the representativeness of result of the test, and the fragrant-flowered garlic maggot susceptibility of gained is poor, result of the test is inaccurate.
The method (ZL200810238713.6) of fragrant-flowered garlic maggot raised by artificial feed before our unit, and can realize indoor unremitting, fragrant-flowered garlic maggot is raised in the impact not by external environment, by the innoxious raising selecting raw material can realize fragrant-flowered garlic maggot.But mainly reached the effect of moisturizing in feeding process by filter paper, need at substantial manually to carry out adding water moisturizing, choose insect, choose ovum, waste time and energy, and the agar added in feed, preservative etc. add the cost of feed.
Summary of the invention
For the existing many deficiencies existed for examination fragrant-flowered garlic maggot raising method, the invention provides a kind of extensive rearing method for examination fragrant-flowered garlic maggot, the existing raising method using the artificial feed of fresh leek, complexity as feed is instead of with the potato easily obtained, after coordinating raising method of the present invention simultaneously, than existing for its survival rate of examination fragrant-flowered garlic maggot raising method, pupation rate, eclosion rate, single female egg laying amount is close, for providing favourable conditions for the regular supply of examination fragrant-flowered garlic maggot.
Rearing method for examination fragrant-flowered garlic maggot of the present invention, specifically comprises the steps:
(1) feed pretreatment: peeled by potato, cleaning, cuts 1-2cm
3block, scalds 10-15 second with boiling water;
(2) selecting is highly the clean container of 10-25cm, and the fine sand of layer overlay 5-7cm within it, fine sand evenly spreads the pretreated feed of one deck, and then is covered with the thin fine sand of one deck 0.5-1cm;
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, grow for Adult worms producting eggs and larval growth, until pupate, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container that feed and fine sand are housed, repeat above step and continue to cultivate.
As everyone knows, the host of fragrant-flowered garlic maggot composes narrow, more single, the mainly liliaceous plant of green onion garlic class, fragrant-flowered garlic maggot harm potato has no report, comprise plant and the fruit of potato, but inventor surprisingly finds under study for action, potato is adopted to do forage feed fragrant-flowered garlic maggot, good effect can be obtained, and potato is root crop, be easy to four seasons preservation, price is low, residue of pesticide are low, especially for agricultural chemicals residual lower of conventional control fragrant-flowered garlic maggot, and it is minimum for the murder by poisoning of fragrant-flowered garlic maggot, ensure that raising continuity, and then ensure that the uniformity supplying examination insect, enhance confidence level and the representativeness of result of the test, result of the test is more accurate.Take food potato for the ease of fragrant-flowered garlic maggot, need to carry out pretreatment to potato, peel by potato, cleaning, cuts 1-2cm
3block, scalds 10-15 second with boiling water.Because this feed is through pretreatment, further avoid the problem of the residue of pesticide of conventional control fragrant-flowered garlic maggot, this ensures that there the safety in feeding process, just ensure that breeding out each can not develop immunity to drugs due to stimulation component such as agricultural chemicals for fragrant-flowered garlic maggot, improve the susceptibility of fragrant-flowered garlic maggot for test, thus improve accuracy rate and the authenticity of Bioexperiment.Simultaneously owing to reducing the dependence for natural leek, make the anxiety that can not cause feed in feeding process because the seasonality of leek stops production, achieve serialization and raise; Potato in the method, the homogeneity of its quality and stability have great raising, this ensures that there the uniformity of fragrant-flowered garlic maggot quality of raising out, the big rise and big fall of fragrant-flowered garlic maggot quality can not be caused due to the relation of feed, for Bioexperiment, this uniformity is of crucial importance.
Above-mentioned feeding process is 25-28 DEG C in temperature, relative moisture is carry out in the box for feeding pests of 75-85%, and because larva grows in the process of adult, temperature and relative moisture have decisive role, in order to prevent fragrant-flowered garlic maggot retarded growth, temperature and relative moisture are controlled in above-mentioned scope.
The present invention has abandoned the method that the conventional means such as employing filter paper, agar, absorbent cotton carry out raising, and have employed fine sand, and fine sand not only can be grown for fragrant-flowered garlic maggot larval growth provides place, and for larval growth provides moist environment, and moistening effect is good.Fine sand is that Adult worms producting eggs provides place simultaneously, avoids transfer and the waste of the ovum of raising method in the past, improves efficiency and the quality of breeding; Fine sand cost is lower, and can recycle, and decreases the cost of raising.The fine sand water content that the present invention adopts is 70%-80%, namely holds agglomerating, but a not water outlet comes, and hand pine group does not scatter entirely.Fine sand particle diameter of the present invention is 0.25-0.35mm.
Fine sand is placed pretreated potato, this feed is rich in protein and each seed amino acid, nutritious, can meet needed for the growth of fragrant-flowered garlic maggot larval growth, can ensure the sustainable supply of nutrition during adult mating, improves efficiency and the quality of breeding; Can recycle simultaneously, decrease the cost of raising.
Clean container of the present invention is generally transparent plastic lunch box, also the large plastic cup after sterilization can be adopted, ensure that the space needed for adult mate and oviposit, larger living space is provided to large quantities of larva, and transparent plastic lunch box, the easy to operate observation facilitating again experimenter.
Although existing raising method is different resolutely from method provided by the present invention, what adopted is all natural leek in the past, shallot or Prof. Du Yucang feed to be to raise fragrant-flowered garlic maggot, the requirement for environmental conditions of raising is higher, and due to shortcomings such as natural leek itself are perishable, change feed, keep humidity etc. to need at substantial manpower.The fresh leek price in winter is higher, and nucleopolyhedrovirus is various, virtually adds feeding cost.The most important thing is that these methods are all unfavorable for extensive raising at present, when needs try worm in a large number time, be difficult to supply.These make existing raising method there is larger defect; Compared with existing disclosed raising method, method of the present invention formally there occurs radical change, have employed modal potato and fine sand raises fragrant-flowered garlic maggot as raw material, has had very large difference between the raising method making the present invention used and existing method.Fine sand moistening effect is good, and appropriateness is more stable, and decrease the number of times manually added water, potato is easily preserved, nutritious, does not need to increase extra composition, and does not almost have residue of pesticide.Therefore this method have cost low, simple to operate, save the advantages such as artificial, and bulky plastic lunch box is that the growth of fragrant-flowered garlic maggot provides the space of breeding on a large scale, therefore can be just 25-28 DEG C in temperature by feeding process, relative moisture is carry out in the insectary of 75-85%, improve the growth rate of fragrant-flowered garlic maggot, compared to prior art can greater amount, faster turn out qualified health for examination fragrant-flowered garlic maggot.
In sum, after adopting above-mentioned raising method, than existing for examination fragrant-flowered garlic maggot raising method, homogeneity and the stability of gained fragrant-flowered garlic maggot quality are enhanced, and its survival rate, pupation rate, eclosion rate, single female egg laying amount and fresh leek, artificial feed are suitable, for providing favourable conditions for the regular supply of examination fragrant-flowered garlic maggot.Improve the susceptibility of fragrant-flowered garlic maggot for test simultaneously, thus improve accuracy rate and the authenticity of Bioexperiment, and this raising method, because the potato adopted is not subject to seasonal restrictions, and be not subject to the impact of remains of pesticide and quality in leek, improve the continuity of raising.
Embodiment
Embodiment 1
For a rearing method for examination fragrant-flowered garlic maggot, it is characterized in that: specifically comprise the steps:
(1) feed pretreatment: peeled by potato, cleaning, cuts 1-2cm
3block, scalded for 10 seconds with boiling water;
(2) selecting is highly the clean container of 25cm, and the fine sand of layer overlay 5cm within it, fine sand evenly spreads the pretreated feed of one deck, and then is covered with the thin fine sand of one deck 1cm;
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, grow for Adult worms producting eggs and larval growth, until pupate, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container that feed and fine sand are housed, repeat above step and continue to cultivate.
In described box for feeding pests, temperature is 25 DEG C, and relative moisture is 85%.
Embodiment 2
For a rearing method for examination fragrant-flowered garlic maggot, it is characterized in that: specifically comprise the steps:
(1) feed pretreatment: peeled by potato, cleaning, cuts 1-2cm
3block, scalded for 15 seconds with boiling water;
(2) selecting is highly the clean container of 10cm, and the fine sand of layer overlay 6cm within it, fine sand evenly spreads the pretreated feed of one deck, and then is covered with the thin fine sand of one deck 0.5cm;
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, grow for Adult worms producting eggs and larval growth, until pupate, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container that feed and fine sand are housed, repeat above step and continue to cultivate.
In described box for feeding pests, temperature is 28 DEG C, and relative moisture is 75%.
Embodiment 3
For a rearing method for examination fragrant-flowered garlic maggot, it is characterized in that: specifically comprise the steps:
(1) feed pretreatment: peeled by potato, cleaning, cuts 1-2cm
3block, scalded for 11 seconds with boiling water;
(2) selecting is highly the clean container of 15cm, and the fine sand of layer overlay 7cm within it, fine sand evenly spreads the pretreated feed of one deck, and then is covered with the thin fine sand of one deck 1cm;
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, grow for Adult worms producting eggs and larval growth, until pupate, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container that feed and fine sand are housed, repeat above step and continue to cultivate.
In described box for feeding pests, temperature is 26 DEG C, and relative moisture is 80%.
Embodiment 4
For a rearing method for examination fragrant-flowered garlic maggot, it is characterized in that: specifically comprise the steps:
(1) feed pretreatment: peeled by potato, cleaning, cuts 1-2cm
3block, scalded for 12 seconds with boiling water;
(2) selecting is highly the clean container of 20cm, and the fine sand of layer overlay 5cm within it, fine sand evenly spreads the pretreated feed of one deck, and then is covered with the thin fine sand of one deck 1cm;
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, grow for Adult worms producting eggs and larval growth, until pupate, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container that feed and fine sand are housed, repeat above step and continue to cultivate.
In described box for feeding pests, temperature is 27 DEG C, and relative moisture is 75%.
Embodiment 5
For a rearing method for examination fragrant-flowered garlic maggot, it is characterized in that: specifically comprise the steps:
(1) feed pretreatment: peeled by potato, cleaning, cuts 1-2cm
3block, scalded for 15 seconds with boiling water;
(2) selecting is highly the clean container of 25cm, and the fine sand of layer overlay 7cm within it, fine sand evenly spreads the pretreated feed of one deck, and then is covered with the thin fine sand of one deck 0.5cm;
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, grow for Adult worms producting eggs and larval growth, until pupate, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container that feed and fine sand are housed, repeat above step and continue to cultivate.
In described box for feeding pests, temperature is 25 DEG C, and relative moisture is 75%.
Comparative example 1
At identical conditions, adopt natural feed (leek) and artificial feed (feed described in ZL200810238713.6) as the method nursing fragrant-flowered garlic maggot described in the conventional raising method of feed and embodiment 1, the survival rate of fragrant-flowered garlic maggot, pupation rate, eclosion rate is as follows with single female egg laying amount result:
As can be seen from above relatively: the fragrant-flowered garlic maggot of the raising method gained adopting embodiment 1 to adopt, survival rate, pupation rate, eclosion rate all exceeds the fragrant-flowered garlic maggot adopting natural feed (leek) and artificial feed (feed described in ZL200810238713.6) as the conventional raising method nursing of feed, although single female egg laying amount, a little less than existing raising method, still can meet the further use in later stage.And the present invention adopts potato as feed, residue of pesticide are few, and the high and feeding cost of safety is well below the raising method adopting artificial feed (feed described in ZL200810238713.6).
Claims (3)
1., for a rearing method for examination fragrant-flowered garlic maggot, it is characterized in that: specifically comprise the steps:
(1) feed pretreatment: peeled by potato, cleaning, cuts 1-2cm
3block, scalds 10-15 second with boiling water;
(2) selecting is highly the clean container of 10-25cm, and the fine sand of layer overlay 5-7cm within it, fine sand evenly spreads the pretreated feed of one deck, and then is covered with the thin fine sand of one deck 0.5-1cm;
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, grow for Adult worms producting eggs and larval growth, until pupate, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container that feed and fine sand are housed, repeat above step and continue to cultivate.
2. raising method according to claim 1, is characterized in that: the fine sand water content in described step (2) is 70%-80%.
3. raising method according to claim 1, is characterized in that: in described box for feeding pests, temperature is 25-28 DEG C, and relative moisture is 75-85%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957116A (en) * | 2015-07-08 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for luring and collecting Chinese chive maggot imagoes for laying eggs |
| CN105145488A (en) * | 2015-07-08 | 2015-12-16 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring Chinese chive maggot larvae |
| CN105918256A (en) * | 2016-03-23 | 2016-09-07 | 中国农业科学院蔬菜花卉研究所 | Simple method for artificial feeding of chinese chive maggots |
| CN107736307A (en) * | 2017-10-24 | 2018-02-27 | 河北省农林科学院植物保护研究所 | A kind of method that fungus gnat is raised using sweet potato |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104957116A (en) * | 2015-07-08 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for luring and collecting Chinese chive maggot imagoes for laying eggs |
| CN105145488A (en) * | 2015-07-08 | 2015-12-16 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring Chinese chive maggot larvae |
| CN104957116B (en) * | 2015-07-08 | 2018-06-15 | 山东省农业科学院植物保护研究所 | A kind of method for trapping fragrant-flowered garlic maggot Adult worms producting eggs |
| CN105145488B (en) * | 2015-07-08 | 2019-09-06 | 山东省农业科学院植物保护研究所 | A method of it traps and monitors bradysia odoriphaga larva |
| CN105918256A (en) * | 2016-03-23 | 2016-09-07 | 中国农业科学院蔬菜花卉研究所 | Simple method for artificial feeding of chinese chive maggots |
| CN107736307A (en) * | 2017-10-24 | 2018-02-27 | 河北省农林科学院植物保护研究所 | A kind of method that fungus gnat is raised using sweet potato |
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