CN101433276A - Artificial synthetic feed containing leek maggot - Google Patents
Artificial synthetic feed containing leek maggot Download PDFInfo
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- CN101433276A CN101433276A CNA2008102387140A CN200810238714A CN101433276A CN 101433276 A CN101433276 A CN 101433276A CN A2008102387140 A CNA2008102387140 A CN A2008102387140A CN 200810238714 A CN200810238714 A CN 200810238714A CN 101433276 A CN101433276 A CN 101433276A
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Abstract
The invention relates to artificial synthetic feed, in particular to artificial synthetic feed of chive maggot. The feed consists of mushroom powder taken as a main composition and yeast powder as an auxiliary material. The feed with the mixture ratio is used to feed the chive maggot; the survival rate, the pupation rate, the eclosion rate and the egg laying amount of a single female are quite close to the chive maggot fed by natural feed; and the average pupae weight of male and female is higher than the chive maggot fed by natural feed, thereby favorable condition for normal supply for the tested chive maggot.
Description
Technical field
The present invention relates to a kind of artificial synthetic diet, be specifically related to a kind of synthetic diet on probation during for artificial propagation fragrant-flowered garlic maggot.
Background technology
Fragrant-flowered garlic maggot---leek eye fungus gnat Bradysia odoriphay late is the No.1 insect of leek, directly threatens and restricting safety in production and the sale of leek.For a long time, the vegetable grower mainly relies on high poison or hypertoxic pesticide, as parathion-methyl, and thimet (3911), acephatemet, the different sulphur phosphorus of methyl, this obstinate insect of control such as furans pellet, even arbitrarily strengthen dose, cause urban and rural residents' poisoning or death by accident often to take place.The Shandong Province is in recent years in Dezhou, diet repeatedly takes place and causes poisoning with leek in Boxing, ground such as Ningjin, wherein the most serious cause 70 surplus people's acute poisoning, the residual hazard problem of leek has caused social worried and concern, has become problem anxious to be solved in the vegetables.
Along with people to the concern of the general level of the health and raising that quality of life is required, in the pollution-free food pollution-free vegetable produce and the cry of agricultural sustainable development more and more stronger.Basic conception and the specification requirement that pollution-free vegetable produces be " for guaranteeing certain output and benefit, production environment is not produced pollute and the agricultural product body under the prerequisite that do not exceed standard of poisonous residuals, allow to limit the quantity of and use the chemical substance of qualification." that is to say that pollution-free vegetable produces, allow to limit the quantity of and use some chemical fertilizer, low-toxin farm chemicals etc., but the poisonous residuals in the vegetables can not surpass state-set standard.Nearest Beijing, special requirement and regulation have all been done to the nuisanceless production of leek in big cities such as Shanghai.
Large scale artificial breeding's technology for the examination insect is one of important means of modern control of insect research.The fragrant-flowered garlic maggot is as research and controlling object, especially carry out research work such as the biological control and resistance to the action of a drug mensuration, steriliation by irradiation, study its artificial synthetic diet and mass rearing reproduction technique, solve examination worm supply problem, to the nuisanceless production of leek is crucial, and the feed that existing fragrant-flowered garlic maggot captive breeding technology is adopted is generally natural leek, so just cause seasonal feed shortage, be unfavorable for the development of fragrant-flowered garlic maggot captive breeding technology.
Summary of the invention
Many deficiencies at existing natural leek feed existence, the invention provides the artificial synthetic diet of a kind of fragrant-flowered garlic maggot, this feed as major ingredients, is aided with auxiliary material compositions such as dusty yeast by mushroom powder, adopt the feed of this proportioning that the fragrant-flowered garlic maggot is raised, its survival rate, percentage of pupation, eclosion rate, single female egg laying amount, all the fragrant-flowered garlic maggot of feeding with natural feed is very approaching, and the average pupa of male and female heavily is higher than the fragrant-flowered garlic maggot that natural feed is fed, for the regular supply for examination fragrant-flowered garlic maggot provides strong condition.
Artificial synthetic diet of the present invention, its proportioning is counted according to weight portion: mushroom powder 150-165 part, dusty yeast 55-65 part adopts water as solvent.
The inventor finds after deliberation, contain multiple nutritional labeling in the mushroom, can satisfy the needs of fragrant-flowered garlic maggot growth fully, but will pester as main nutriment and after forming feed, if the content of mushroom is low excessively, or cause the nutritional labeling deficiency, and cause the death of fragrant-flowered garlic maggot, after the same consumption when mushroom is too high, the meeting toxigenicity, thus cause fragrant-flowered garlic maggot food poisoning and dead, and therefore the consumption with mushroom powder is limited in weight portion 150-165 part, preferred employing 155-157 part, the amount of selecting dusty yeast simultaneously for use is 60-63 part; And be that best proportioning adopts 157 parts of mushroom powders, 63 parts of dusty yeasts concrete the use.
Owing to pester unlike leek for too high restriction is arranged season, and nutritional labeling is more abundant than leek, therefore the quick healthy growth that helps the fragrant-flowered garlic maggot, can adopt use mushroom commonly used during concrete the use, as mushroom, straw mushroom, flat mushroom and mushroom etc., and in use, whole thalline of whole mushroom all can be processed into mushroom powder and use, reduced production cost, in use no matter the fresh mushroom or the dried mushrooms that adopt, all the weight with its dry weight is the use standard, prevents in the fresh mushroom moisture more and cause the deficiency of nutriment.
Except above-mentioned major ingredient, also be added with in this synthetic diet: anticorrisive agent 4-6 part, agar 20-30 part.
Wherein anticorrisive agent adopts benzoic acid or Sodium Benzoate or sorbic acid or its mixture, and when adopting three's mixture, its weight ratio is 1.2-1.3: 3.5-4: 1, and preferred weight ratio is 1.25: 3.75: 1.Except above-mentioned various anticorrisive agents, can also adopt the various edible anticorrisive agents on the market, can effectively prolong the resting period of artificial synthetic diet after the employing anticorrisive agent, made things convenient for the mass rearing of each time period, controlled this consumption simultaneously and can not produce again and poison and the growth of fragrant-flowered garlic maggot is exerted an influence for the fragrant-flowered garlic maggot.Adopt agar as additive, at first can be used as stabilizing agent each component in the above-mentioned prescription is stablized.
Add man-hour, concrete steps are as follows:
(1) with mushroom powder, dusty yeast is mixing in proportion, adds the water of 2-3 times of weight, stirs;
(2) agar powder is proportionally joined in the water of 15-25 times of weight, after the heating for dissolving, proportionally add anticorrisive agent again, stir, make agar solution;
(3) agar solution that makes in (2) is poured in the mixed liquor that makes in (1), stirred, it is standby that feed is made in cooling.
The artificial synthetic diet that adopts this method to make, wherein each kind of nutritional labeling can not influence each other, can play coordinative role preferably, simultaneously owing to adopted anti-corrosion measure preferably, make the holding time of feed further prolong, reached all-year uninterrupted use, reduced degree of dependence for pure natural leek feed, utilized the major ingredient of this more handy resource of pestering simultaneously as feed, the diversity of abundant fragrant-flowered garlic maggot feed, the while also provides contrast preferably for the culture effect of fragrant-flowered garlic maggot under the different feeding environment.
In sum, the novel synthetic fragrant-flowered garlic maggot feed that adopts said ratio and processing method to make, effectively reduced for the degree of dependence of examination fragrant-flowered garlic maggot raising for leek, realized that whole year is for the raising for examination fragrant-flowered garlic maggot, this synthetic diet is to raising for examination fragrant-flowered garlic maggot simultaneously, its survival rate, percentage of pupation, eclosion rate, single female egg laying amount, all the fragrant-flowered garlic maggot of feeding with natural feed is very approaching, and the average pupa of male and female heavily is higher than the fragrant-flowered garlic maggot that natural feed is fed, for the regular supply for examination fragrant-flowered garlic maggot provides strong condition.Owing to adopted mushroom powder as main nutriment, both guaranteed that each nutritional labeling had enough amounts, also reached mutual balance to each other; Owing to can not contain residual components such as agricultural chemicals in the artificial synthetic feed, therefore improved survival rate and the breeding potential of fragrant-flowered garlic maggot, reduced the risk of breeding.
The specific embodiment
Embodiment 1
A kind of synthetic diet on probation during for artificial propagation fragrant-flowered garlic maggot, its proportioning is: mushroom powder 9g, leek powder 2g, dusty yeast 3.36g, agar powder 1.25g, benzoic acid 0.05g, Sodium Benzoate 0.15g, honey 0.25g, sorbic acid 0.04g.
The concrete compound method of feed is:
(1) with mushroom powder, leek powder, dusty yeast be according to aforementioned proportion, adds in 35 ml waters to stir;
(2) agar powder with aforementioned proportion adds in 25 ml waters, after the heating for dissolving, adds benzoic acid again, Sodium Benzoate, and sorbic acid and honey stir, and make agar solution;
(3) with agar solution to going in the mixed liquor that makes in (1), stir, to make feed standby in cooling.
Embodiment 2
A kind of synthetic diet on probation during for artificial propagation fragrant-flowered garlic maggot, its proportioning is: mushroom powder 8g, dusty yeast 2.81g, agar powder 1.45g, benzoic acid 0.06g, Sodium Benzoate 0.18g, sorbic acid 0.05g.
The concrete compound method of feed is:
(1) with mushroom powder and dusty yeast according to aforementioned proportion, add in 31 ml waters and stir;
(2) agar powder with aforementioned proportion adds in 36 ml waters, after the heating for dissolving, adds benzoic acid again in micro-wave oven, and Sodium Benzoate and sorbic acid stir, and make agar solution;
(3) with agar solution to going in the mixed liquor that makes in (1), stir, to make feed standby in cooling.
Embodiment 3
A kind of synthetic diet on probation during for artificial propagation fragrant-flowered garlic maggot, its proportioning is: mushroom powder 9g, leek powder 2g, dusty yeast 3.38g.
The concrete compound method of feed is:
(1) with mushroom powder, leek powder, dusty yeast be according to aforementioned proportion, adds in 37 ml waters to stir, and it is standby that feed is made in cooling.
Embodiment 4
A kind of synthetic diet on probation during for artificial propagation fragrant-flowered garlic maggot, its proportioning is: mushroom powder 10g, dusty yeast 3.81g, agar powder 1.48g, benzoic acid 0.05g, Sodium Benzoate 0.16g, sorbic acid 0.04g.
The concrete compound method of feed is:
(1) with mushroom powder, dusty yeast is according to aforementioned proportion, adds in 39 ml waters to stir;
(2) agar powder with aforementioned proportion adds in 26 ml waters, after the heating for dissolving, adds benzoic acid again in micro-wave oven, and Sodium Benzoate and sorbic acid stir, and make agar solution;
(3) with agar solution to going in the mixed liquor that makes in (1), stir, to make feed standby in cooling.
Embodiment 5
A kind of synthetic diet on probation during for artificial propagation fragrant-flowered garlic maggot, its proportioning is: mushroom powder 9g, dusty yeast 2.81g, benzoic acid 0.07g, Sodium Benzoate 0.21g, honey 0.1g, sorbic acid 0.05g.
The concrete compound method of feed is:
(1) with mushroom powder, dusty yeast is according to aforementioned proportion, adds in 24 ml waters to stir;
(2) in the mixing material of aforementioned proportion, add benzoic acid, Sodium Benzoate, sorbic acid and honey stir, and it is standby that feed is made in cooling.
Embodiment 6
A kind of synthetic diet on probation during for artificial propagation fragrant-flowered garlic maggot, its proportioning is: mushroom powder 9g, dusty yeast 3.36g.
The concrete compound method of feed is:
With mushroom powder, dusty yeast is according to aforementioned proportion, adds in 24 ml waters to stir; It is standby that feed is made in cooling.
Comparative example:
Under identical condition, feed the fragrant-flowered garlic maggot with the artificial synthetic diet 1 of natural feed (leek) and employing embodiment 2 used proportionings, each is handled and repeats 10 times.Connect 200～300 ovum in each culture dish, guarantee that the interior larva number of each culture dish is greater than 100.Every day in pupa time, 24h divided three periods to observe, and in time collected pupa, performed record, every kind of processing is repeated resulting pupa all differentiate male and female, divided another name male and female pupa heavy.Then with the every kind of pupa that arrives mixing of handling to repeat institute for 10 times, therefrom get 100 pairs of male and female pupas or 200 pairs at random, each is that the culture dish of 3cm is laid eggs to putting into diameter, culture dish is spread moistening filter paper, adds the cotton pellet of being with hydromel.Calculate the survival rate of fragrant-flowered garlic maggot, percentage of pupation, eclosion rate, the heavy and single female egg laying amount of pupa.The results are shown in following table
Table is raised the effect of fragrant-flowered garlic maggot
By comparative example as can be seen: the fragrant-flowered garlic maggot that the feed 1 that adopts embodiment 2 to make is fed, survival rate, percentage of pupation, eclosion rate, single female egg laying amount, all the fragrant-flowered garlic maggot of feeding with natural feed is very approaching and all have and exceed, and also all is higher than the fragrant-flowered garlic maggot that natural feed is fed and the average pupa of male and female is heavy.But since natural feed to be subjected to the influence in season very big, therefore can't use for a long time, but not fresh leek is because its loss of nutrient substances, its effect has bigger gap than artificial synthetic diet of the present invention; All contain residues of pesticides in the simultaneously fresh leek feed, security is much lower than synthetic diet of the present invention in use.
Claims (6)
1. artificial synthetic diet of fragrant-flowered garlic maggot, it is characterized in that: its proportioning is counted according to weight portion: mushroom powder 150-165 part, dusty yeast 55-65 part.
2. synthetic diet according to claim 1 is characterized in that: its proportioning is counted according to weight portion: mushroom powder 155-159 part, dusty yeast 60-65 part.
3. synthetic diet according to claim 1 and 2 is characterized in that: its proportioning also comprises by weight: anticorrisive agent 4-6 part, agar 20-30 part.
4. synthetic diet according to claim 3 is characterized in that: described anticorrisive agent is benzoic acid or Sodium Benzoate or sorbic acid or its mixture, and when adopting three's mixture, its weight ratio is 1.2-1.3:3.5-4:1.
5. synthetic diet according to claim 4 is characterized in that: when the mixture that adopts benzoic acid, Sodium Benzoate and sorbic acid during as anticorrisive agent, the weight ratio that the three mixes is 1.25:3.75:1.
6. process the method for synthetic diet according to claim 1, it is characterized in that: mainly comprise the steps:
(1) with mushroom powder, dusty yeast is mixing in proportion, adds the water of 2-3 times of weight, stirs;
(2) agar powder is proportionally joined in the water of 15-25 times of weight, after the heating for dissolving, proportionally add anticorrisive agent again, stir, make agar solution;
(3) agar solution that makes in (2) is poured in the mixed liquor that makes in (1), stirred, it is standby that feed is made in cooling.
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| CN103385386A (en) * | 2013-07-22 | 2013-11-13 | 山东省农业科学院植物保护研究所 | Artificial synthesized feed for proxenuslepigone moschler and preparation method thereof |
| CN103399126A (en) * | 2013-08-02 | 2013-11-20 | 山东农业大学 | Indoor bioassay method of bradysia odoriphaga larvae |
| CN104509494A (en) * | 2014-12-16 | 2015-04-15 | 山东省农业科学院植物保护研究所 | Artificial rearing method of tested Chinese chive maggot |
| CN104542498A (en) * | 2014-12-16 | 2015-04-29 | 山东省农业科学院植物保护研究所 | Artificial feeding method for tested bradysia odoriphaga |
| CN104664155A (en) * | 2015-02-10 | 2015-06-03 | 长江大学 | Culture medium for manually and rapidly feeding Chinese chive maggots and preparation method of culture medium |
| CN104957116A (en) * | 2015-07-08 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for luring and collecting Chinese chive maggot imagoes for laying eggs |
| CN105028361A (en) * | 2015-07-08 | 2015-11-11 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring adult Chinese chive maggots |
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| CN103385386B (en) * | 2013-07-22 | 2014-12-17 | 山东省农业科学院植物保护研究所 | Artificial synthesized feed for proxenuslepigone moschler and preparation method thereof |
| CN103340181A (en) * | 2013-07-22 | 2013-10-09 | 山东省农业科学院植物保护研究所 | Method for artificially rearing athetis lepigone for test |
| CN103399126A (en) * | 2013-08-02 | 2013-11-20 | 山东农业大学 | Indoor bioassay method of bradysia odoriphaga larvae |
| CN104542498B (en) * | 2014-12-16 | 2016-11-30 | 山东省农业科学院植物保护研究所 | A kind of rearing method for examination fragrant-flowered garlic maggot |
| CN104509494A (en) * | 2014-12-16 | 2015-04-15 | 山东省农业科学院植物保护研究所 | Artificial rearing method of tested Chinese chive maggot |
| CN104542498A (en) * | 2014-12-16 | 2015-04-29 | 山东省农业科学院植物保护研究所 | Artificial feeding method for tested bradysia odoriphaga |
| CN104509494B (en) * | 2014-12-16 | 2017-01-25 | 山东省农业科学院植物保护研究所 | Artificial rearing method of tested Chinese chive maggot |
| CN104664155A (en) * | 2015-02-10 | 2015-06-03 | 长江大学 | Culture medium for manually and rapidly feeding Chinese chive maggots and preparation method of culture medium |
| CN105028361A (en) * | 2015-07-08 | 2015-11-11 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring adult Chinese chive maggots |
| CN104957116A (en) * | 2015-07-08 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for luring and collecting Chinese chive maggot imagoes for laying eggs |
| CN104957116B (en) * | 2015-07-08 | 2018-06-15 | 山东省农业科学院植物保护研究所 | A kind of method for trapping fragrant-flowered garlic maggot Adult worms producting eggs |
| CN105918256A (en) * | 2016-03-23 | 2016-09-07 | 中国农业科学院蔬菜花卉研究所 | Simple method for artificial feeding of chinese chive maggots |
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