CN100508778C - Artificial feed for tubular breeding longhorn beetle larva and breeding method thereof - Google Patents
Artificial feed for tubular breeding longhorn beetle larva and breeding method thereof Download PDFInfo
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- CN100508778C CN100508778C CNB2006100178620A CN200610017862A CN100508778C CN 100508778 C CN100508778 C CN 100508778C CN B2006100178620 A CNB2006100178620 A CN B2006100178620A CN 200610017862 A CN200610017862 A CN 200610017862A CN 100508778 C CN100508778 C CN 100508778C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses an artificial fodder for feeding insect larvae of capricorn beetle, as well as the method for feeding, wherein the fodder comprises (by weight portions) agar 3-9 parts, edible plant oil 2-5 parts, 10% acetic acid 1-3 parts, 75% alcohol 2-4 parts, food preservative agent 1-3 parts, cellulose 2-8 parts, composite vitamins 1-4 parts, vitamin C 0.2-0.6 part, wheat bran 40-80 parts, yeast powder 2-8 parts, and water 100-300 parts.
Description
One, technical field: the present invention relates to a kind of insect method for breeding, particularly relate to a kind of artificial feed for tubular breeding longhorn beetle larva and method for breeding thereof.
Two, background technology: Cerambycidae Cerambycidae, belong to coleoptera Coleoptera, endanger forest and fruit tree more, minority harms the crops and fiber crops, bamboo, flowers etc., individual species harm timber and wood furniture etc. are the important pests of forestry, agricultural and building industry.Roundheaded borer phase moth food trunk, branch and root has part harm herbaceous plant, and larva moves in stem or the root, and a few species moves in and gets the food root in the soil.For example chrysanthemum little tube longicorn (Phytoecia rufiventris Gautier) has another name called chrysanthemum tiger, chrysanthemum longicorn, chrysanthemum marrow longicorn, be commonly called as " ocean folder ", moth bar insect, Phytoecia, the main harm feverfew, the host has multiple chrysanthemum, Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait, elm, European lotus, Ai Najin, Artemisia capillaris, absinth, mattress wormwood artemisia, Atractylis chinensis, the bighead atractylodes rhizome, marihuana, annual fleabane herb, Herba Lycopi, aster etc.Along with the adjustment of the structure of agricultural production, the international exchange and cooperation joint venture increased in recent years, the continuous introduction of plant variety and the extensive plantation of characteristic edible wild herbs, and longicorn harm is on the rise.Because the problems such as the resistance to the action of a drug that environmental pressure that the use chemical prevention and control method causes and insect produce utilize additive method to control the focus that this worm has become current control.Eat into the insect of property because of mostly roundheaded borer is brill, the chemical prevention effect is relatively poor, and contaminated environment, and biological control will be the effective measures of sustainable development.
Longicorn life cycle is longer, causes the artificial feeding difficulty, do not have ready-made method can reference, particularly fungi and the production and the utilization of bacterial micro-organism agricultural chemicals be restricted.At these problems, be a large amount of breeding longicorns, biological toxicity test test material not only is provided, and the protein food of breeding longhorn beetle develop and useedd technical support is provided, provide basis, worm source to producing microbial pesticide simultaneously, we have carried out invention research to the method for breeding of breeding longhorn beetle and man-made feeds prescription and preparation method.
Three, summary of the invention:
The object of the present invention is to provide man-made feeds of a kind of tubular breeding longhorn beetle and preparation method thereof, the present invention also aims to provide a kind of method for breeding of tubular breeding longhorn beetle.
Technical scheme of the present invention:
A kind of artificial feed for tubular breeding longhorn beetle larva is a unit with the weight portion, contains agar 3-9 part in the prescription, edible vegetable oil 2-5 part, 10% acetate 1-3 part, 75% alcohol 2-4 part, food is used anticorrisive agent 1-3 part, cellulose 2-8 part, B B-complex 1-4 part, vitamin C 0.2-0.6 part, wheat bran 40-80 part, dusty yeast 2-8 part, water 100-400 part.
Described artificial feed for tubular breeding longhorn beetle larva, agar 4-7 part wherein, edible vegetable oil 2-4 part, 10% acetate 1-3 part, 75% alcohol 2-4 part, food is used anticorrisive agent 1-3 part, cellulose 4-6 part, B B-complex 1-3 part, vitamin C 0.2-0.4 part, wheat bran 50-70 part, dusty yeast 3-6 part, water 150-260 part.
Described edible vegetable oil is any in peanut oil, corn oil, rape seed oil, the soybean oil, or any two combinations, or two or more any combinations.
Described food anticorrisive agent is a sorbic acid, or potassium sorbate, or in the Sodium Benzoate any.
Described cellulose is chemicals cellulose or crude fibre, and described B B-complex is a vitamin B compound.
A kind of preparation method of artificial feed for tubular breeding longhorn beetle larva is:
1) get 100-400 part water with clean container, to wherein adding agar 3-9 part, heating is also constantly stirred, and boils 3-5 minute, treats that agar dissolves fully, and it is cooled to 60～80 ℃;
2) in container, add vegetable edible oil 2-5 part successively, wheat bran 40-80 part, 10% acetate 1-3 part, 75% alcohol 2-4 part, food is used anticorrisive agent 1-3 part, cellulose 2-8 part, B B-complex 2-4 part, vitamin C 0.2-0.6 part, dusty yeast 2-8 part is stirred with thin rod while feeding in raw material;
3) stir, be cooled to room temperature, promptly obtain the roundheaded borer feed.
Described thin rod is aseptic thin glass bar, or aseptic thin stick.
A kind of method for breeding of tubular breeding longhorn beetle, described man-made feeds are packed into support in the scolite, compacting, in the middle of feed, bore a duct with thin rod, and on lid, make a call to an air-vent, roundheaded borer is placed in the middle duct of feed carefully, rests scolite then and place growth cabinet to raise.
The method for breeding of described tubular breeding longhorn beetle, man-made feeds are packed in the cleaning of diameter 1-3cm, the long 5-10cm plastic tube or glass tube with cover, be filled to pipe 3/5-4/5 place until feed, compacting, the duct that is equivalent to 3-4 instar larvae body thickness with thin rod brill one in the middle of feed approximately, and on lid, make a call to an air-vent, roundheaded borer is placed in the middle duct of feed carefully, resting scolite then places growth cabinet to raise, changed fresh feed every 7-10 days, larva freely grows in pipe and gets food, pupates until turning into adult.
Described thin rod is aseptic thin glass bar, or aseptic thin stick.
Positive beneficial effect of the present invention:
1) man-made feeds comprehensive nutrition of the present invention, cost of material is cheap, and its cost is low, remarkable in economical benefits.
2) man-made feeds preparation method of the present invention is simple, easy to operate, and laboratory technician or member of ordinary skill can finish feed preparation work under no special installation condition.
3) feed of the present invention can be raised multiple roundheaded borer, has very strong practicality.As can raising the little tube of mulberry borer, longicorn beetle class, chrysanthemum longicorn etc., and the roundheaded borer of raising can be used as seafood or experiment material, also can be used for producing the raw material of microbial pesticide or compound protein additive.
4) man-made feeds of the present invention are in order to raise roundheaded borer, and bionical attitude is raised, and the survival rate height is grown more neatly, and feeding effect is more stable.
5) roundheaded borer method for breeding of the present invention is convenient to test indoor feeding and is observed its history of life, and the aspect operation is easily grasped, and manages little not waste feed, and easily changes feed.
6) the foster scolite low price of using in the roundheaded borer method for breeding of the present invention can be reused, and feeding cost is lower, and foster scolite can be concentrated placement, and it is less to take up space.
Four, the specific embodiment:
Embodiment one:
1. artificial feed for tubular breeding longhorn beetle larva prescription:
With the weight portion is unit, 4 parts in agar, 3 parts of peanut oil, 1 part of 10% acetate, 2 parts of 75% alcohol, 2 parts of sorbic acids, 4 parts of chemicals celluloses, 2 parts of vitamin B compounds, 0.2 part of vitamin C, 50 parts in wheat bran, 4 parts of dusty yeasts, 100 parts in water;
2. the concrete preparation steps of man-made feeds is:
1) get 100 parts water with clean beaker, add 4 parts in agar, heating is also constantly stirred, and boils 3 minutes, treats that agar dissolves fully, is cooled to 60 ℃;
2) add 3 parts of peanut oil then successively, 50 parts in wheat bran, 1 part of 10% acetate, 2 parts of 75% alcohol, 4 parts of chemicals celluloses, 2 parts of vitamin B compounds, 0.2 part of vitamin C, 2 parts of sorbic acids, 4 parts of dusty yeasts stir while feeding in raw material with aseptic thin glass bar;
3) stir, be cooled to room temperature, obtain the roundheaded borer feed, the foster scolite of can packing into.
4) be distributed into bag,, can deposit 6～8 days airtight depositing below 4 ℃.
3. the method for breeding of tubular breeding longhorn beetle:
Above-mentioned man-made feeds are packed into support in the scolite, compacting is bored a duct with thin rod, and make a call to an air-vent on lid in the middle of feed, roundheaded borer is placed in the middle duct of feed carefully, rests scolite then and places growth cabinet to raise.
Embodiment two:
1. artificial feed for tubular breeding longhorn beetle larva prescription:
With the weight portion is unit, 5 parts in agar, 4 parts of corn oils, 2 parts of 10% acetate, 2 parts of 75% alcohol, 3 parts of potassium sorbates, 6 parts of chemicals celluloses, 3 parts of vitamin B compounds, 0.4 part of vitamin C, 70 parts in wheat bran, 5 parts of dusty yeasts, 200 parts in water;
2. artificial feed for tubular breeding longhorn beetle larva preparation steps:
1) get 200 parts water with clean plastic tub, add 5 parts in agar, heating is also constantly stirred, and boils 5 minutes, treats that agar dissolves fully, is cooled to 80 ℃;
2) add 4 parts of corn oils then successively, 70 parts in wheat bran, 2 parts of 10% acetate, 2 parts of 75% alcohol, 6 parts of chemicals celluloses, 3 parts of vitamin B compounds, 0.4 part of vitamin C, 3 parts of potassium sorbates, 5 parts of dusty yeasts stir while feeding in raw material with aseptic thin stick;
3) stir, be cooled to room temperature, obtain the roundheaded borer feed, the foster scolite of can packing into.
4) be distributed into bag,, can deposit 6～8 days airtight depositing below 4 ℃.
3. the method for breeding of tubular breeding longhorn beetle: the man-made feeds of being joined are packed in the cleaning of diameter 3cm, the long 10cm glass tube with cover, be filled to pipe 4/5 place until feed, compacting, the duct that is equivalent to 3-4 instar larvae body thickness with thin rod brill one in the middle of feed approximately, and on lid, make a call to an air-vent, roundheaded borer is placed in the middle duct of feed carefully, resting scolite then places growth cabinet to raise, changed fresh feed every 10 days, larva freely grows in pipe and gets food, pupates until turning into adult.
Embodiment three:
1. artificial feed for tubular breeding longhorn beetle larva prescription,
8 parts in agar, 5 parts of rape seed oils, 2 parts of 10% acetate, 3 parts of 75% alcohol, 3 parts in benzoic acid, 8 parts of crude fibres, 3 parts of vitamin B compounds, 0.5 part of vitamin C, 80 parts in wheat bran, 6 parts of dusty yeasts, 300 parts in water.
2. press the step of above-mentioned formulated man-made feeds:
1) with 300 parts of clean plastic tub water intakings, add 8 parts in agar, heating is also constantly stirred, and boils 4 minutes, treats that agar dissolves fully, is cooled to 70 ℃;
2) add corn oil by prescription successively then, wheat bran, 10% acetate, 75% alcohol, the chemicals cellulose, vitamin B compound, vitamin C, potassium sorbate, dusty yeast stirs while feeding in raw material with aseptic thin stick;
3) stir, be cooled to room temperature, obtain the roundheaded borer feed, the foster scolite of can packing into.
4) be distributed into bag,, can deposit 6～8 days airtight depositing below 4 ℃.
3. the method for breeding of tubular breeding longhorn beetle: the man-made feeds of being joined are packed in the cleaning of diameter 1cm, the long 5cm plastic tube with cover, be filled to pipe 3/5 place until feed, compacting, the duct that is equivalent to 3-4 instar larvae body thickness with thin rod brill one in the middle of feed approximately, and on lid, make a call to an air-vent, roundheaded borer is placed in the middle duct of feed carefully, resting scolite then places growth cabinet to raise, changed fresh feed every 7 days, larva freely grows in pipe and gets food, pupates until turning into adult.
Embodiment four:
1. artificial feed for tubular breeding longhorn beetle larva prescription,
3 parts in agar, 2 parts of rape seed oils, 1 part of 10% acetate, 2 parts of 75% alcohol, 1 part in benzoic acid, 2 parts of crude fibres, 1 part of vitamin B compound, 0.2 part of vitamin C, 40 parts in wheat bran, 2 parts of dusty yeasts, 120 parts in water.
2. the artificial feed for tubular breeding longhorn beetle larva preparation steps no longer repeats with embodiment one.
3. the method for breeding of tubular breeding longhorn beetle: the man-made feeds of being joined are packed in the cleaning of diameter 2cm, the long 8cm plastic tube with cover, be filled to pipe 3/5 place until feed, compacting, the duct that is equivalent to 3-4 instar larvae body thickness with aseptic thin glass bar brill one in the middle of feed approximately, and on lid, make a call to an air-vent, roundheaded borer is placed in the middle duct of feed carefully, resting scolite then places growth cabinet to raise, changed fresh feed every 8 days, larva freely grows in pipe and gets food, pupates until turning into adult.
Embodiment five: the artificial feed for tubular breeding longhorn beetle larva prescription is a unit with the weight portion,
9 parts in agar, 5 parts of soybean oils, 3 parts of 10% acetate, 4 parts of 75% alcohol, 3 parts of Sodium Benzoates, 8 parts of crude fibres, 4 parts of vitamin B compounds, 0.6 part of vitamin C, 80 parts in wheat bran, 8 parts of dusty yeasts, 300 parts in water.
Artificial feed for tubular breeding longhorn beetle larva preparation steps and method for breeding no longer repeat with embodiment two.
Embodiment six: the artificial feed for tubular breeding longhorn beetle larva prescription is a unit with the weight portion, 7 parts in agar, 4 parts of soybean oils, 2 parts of 10% acetate, 4 parts of 75% alcohol, 2 parts of Sodium Benzoates, 7 parts of crude fibres, 3 parts of vitamin B compounds, 0.5 part of vitamin C, 70 parts in wheat bran, 6 parts of dusty yeasts, 280 parts in water.
Artificial feed for tubular breeding longhorn beetle larva preparation steps and method for breeding no longer repeat with embodiment two.
Embodiment seven: the artificial feed for tubular breeding longhorn beetle larva prescription is a unit with the weight portion, 4 parts in agar, 4 parts of soybean oils, 2 parts of 10% acetate, 3 parts of 75% alcohol, 2 parts of potassium sorbates, 3 parts of crude fibres, 2 parts of vitamin B compounds, 0.4 part of vitamin C, 50 parts in wheat bran, 3 parts of dusty yeasts, 120 parts in water.
Artificial feed for tubular breeding longhorn beetle larva preparation steps and method for breeding no longer repeat with embodiment three.
Embodiment eight: artificial feed for tubular breeding longhorn beetle larva prescription, 7 parts in agar, 3 parts of peanut oil, 2 parts of 10% acetate, 3 parts of 75% alcohol, 2 parts of potassium sorbates, 6 parts of crude fibres, 2 parts of vitamin B compounds, 0.4 part of vitamin C, 60 parts in wheat bran, 5 parts of dusty yeasts, 350 parts in water, 7 parts of sawdust powder.
Sawdust powder adds with wheat bran during the feed preparation, and all the other steps no longer repeat with embodiment one.
Embodiment nine: the artificial feed for tubular breeding longhorn beetle larva prescription is a unit with the weight portion, 7 parts in agar, 3 parts of peanut oil, 2 parts of 10% acetate, 3 parts of 75% alcohol, 2 parts of potassium sorbates, 6 parts of crude fibres, 2 parts of vitamin B compounds, 0.4 part of vitamin C, 60 parts in wheat bran, 5 parts of dusty yeasts, 200 parts in water, 0.05 part in antibiotic.
Antibiotic adds after feed is cooled to room temperature during the feed preparation, and stirs evenly, and all the other steps no longer repeat with embodiment one.
Embodiment ten: the artificial feed for tubular breeding longhorn beetle larva prescription is a unit with the weight portion, 7 parts in agar, 3 parts of peanut oil, 2 parts of 10% acetate, 3 parts of 75% alcohol, 2 parts of potassium sorbates, 6 parts of crude fibres, 2 parts of vitamin B compounds, 0.4 part of vitamin C, 60 parts in wheat bran, 5 parts of dusty yeasts, 250 parts in water, 0.05 part in penicillin, 5 parts of sawdust powder.
Feed when preparation sawdust powder adds with wheat bran, and penicillin adds after feed is cooled to room temperature and stirs evenly, and all the other steps do not repeat with embodiment two.
Embodiment 11: the artificial feed for tubular breeding longhorn beetle larva prescription is a unit with the weight portion, 4 parts in agar, 5 parts of rape seed oils, 2 parts of 10% acetate, 4 parts of 75% alcohol, 1 part of potassium sorbate, 4 parts of chemicals celluloses, 3 parts of vitamin B compounds, 0.3 part of vitamin C, 45 parts in wheat bran, 6 parts of dusty yeasts, 350 parts in water, 0.1 part in penicillin, 10 parts of sawdust powder.
Feed when preparation sawdust powder adds with wheat bran, and penicillin adds after feed is cooled to room temperature and stirs evenly, and all the other steps do not repeat with embodiment three.
Embodiment 12: the artificial feed for tubular breeding longhorn beetle larva prescription is a unit with the weight portion, 5 parts in agar, 3 parts of peanut oil, 3 parts of 10% acetate, 3 parts of 75% alcohol, 2 parts of potassium sorbates, 4 parts of crude fibres, 3 parts of vitamin B compounds, 0.3 part of vitamin C, 65 parts in wheat bran, 6 parts of dusty yeasts, 400 parts in water, 0.08 part in penicillin, 10 parts of sawdust powder.
Feed when preparation sawdust powder adds with wheat bran, and penicillin adds after feed is cooled to room temperature and stirs evenly, and all the other steps do not repeat with embodiment three.
Claims (10)
1. artificial feed for tubular breeding longhorn beetle larva, it is characterized in that: with the weight portion is unit, contains agar 3-9 part in the prescription, edible vegetable oil 2-5 part, 10% acetate 1-3 part, 75% alcohol 2-4 part, food is used anticorrisive agent 1-3 part, cellulose 2-8 part, vitamin B compound 1-4 part, vitamin C 0.2-0.6 part, wheat bran 40-80 part, dusty yeast 2-8 part, water 100-400 part.
2. artificial feed for tubular breeding longhorn beetle larva according to claim 1, it is characterized in that: agar 4-7 part wherein, edible vegetable oil 2-4 part, 10% acetate 1-3 part, 75% alcohol 2-4 part, food is used anticorrisive agent 1-3 part, cellulose 4-6 part, vitamin B compound 1-3 part, vitamin C 0.2-0.4 part, wheat bran 50-70 part, dusty yeast 3-6 part, water 150-260 part.
3. artificial feed for tubular breeding longhorn beetle larva according to claim 1 and 2 is characterized in that: described edible vegetable oil is any in peanut oil, corn oil, rape seed oil, the soybean oil, or any two combinations, or two or more any combinations.
4. artificial feed for tubular breeding longhorn beetle larva according to claim 1 and 2 is characterized in that: described food anticorrisive agent is a sorbic acid, or potassium sorbate, or in the Sodium Benzoate any.
5. artificial feed for tubular breeding longhorn beetle larva according to claim 1 and 2 is characterized in that: described cellulose is chemicals cellulose or crude fibre.
6. the preparation method of an artificial feed for tubular breeding longhorn beetle larva comprises raw material is mixed, and stirs, and it is characterized in that:
1) get 100-400 part water with clean container, to wherein adding agar 3-9 part, heating is also constantly stirred, and boils 3-5 minute, treats that agar dissolves fully, and it is cooled to 60～80 ℃;
2) in container, add vegetable edible oil 2-5 part successively, wheat bran 40-80 part, 10% acetate 1-3 part, 75% alcohol 2-4 part, food is used anticorrisive agent 1-3 part, cellulose 2-8 part, vitamin B compound 2-4 part, vitamin C 0.2-0.6 part, dusty yeast 2-8 part is stirred with thin rod while feeding in raw material;
3) stir, be cooled to room temperature, promptly obtain the roundheaded borer feed.
7. the preparation method of artificial feed for tubular breeding longhorn beetle larva according to claim 6, it is characterized in that: described thin rod is aseptic thin glass bar, or aseptic thin stick.
8. the method for breeding of a tubular breeding longhorn beetle comprises that man-made feeds pack into to support in the scolite to raise insect, and the periodic replacement feed, it is characterized in that:
The described artificial feed for tubular breeding longhorn beetle larva of claim 1 packed into support in the scolite, compacting is bored a duct with thin rod, and make a call to an air-vent on lid in the middle of feed, roundheaded borer is placed in the middle duct of feed carefully, rests scolite then and place growth cabinet to raise.
9. the method for breeding of tubular breeding longhorn beetle according to claim 8, it is characterized in that: with the man-made feeds diameter 1-3cm that packs into, in the plastic tube or glass tube that the cleaning of long 5-10cm is with cover, be filled to pipe 3/5-4/5 place until feed, compacting, the duct that is equivalent to 3-4 instar larvae body thickness with thin rod brill one in the middle of feed approximately, and on lid, make a call to an air-vent, roundheaded borer is placed in the middle duct of feed carefully, resting scolite then places growth cabinet to raise, changed fresh feed every 7-10 days, larva freely grows in pipe and gets food, pupates until turning into adult.
10. the method for breeding of tubular breeding longhorn beetle according to claim 8, it is characterized in that: described thin rod is aseptic thin glass bar, or aseptic thin stick.
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| CNB2006100178620A CN100508778C (en) | 2006-05-30 | 2006-05-30 | Artificial feed for tubular breeding longhorn beetle larva and breeding method thereof |
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| CNB2006100178620A CN100508778C (en) | 2006-05-30 | 2006-05-30 | Artificial feed for tubular breeding longhorn beetle larva and breeding method thereof |
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| CN100508778C true CN100508778C (en) | 2009-07-08 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101433276B (en) * | 2008-12-22 | 2011-10-05 | 山东省农业科学院植物保护研究所 | Artificial synthetic feed containing leek maggot |
| CN102090377A (en) * | 2010-12-22 | 2011-06-15 | 金陵科技学院 | Simple method for breeding Apriona germari based on field infestation |
| CN102318758B (en) * | 2011-08-19 | 2012-10-31 | 天津北林新苑绿化工程有限公司 | Artificial feedstuff for thyestillagebleri larvae, and preparation method thereof |
| CN102613411B (en) * | 2012-04-09 | 2013-04-10 | 天津师范大学 | Artificial feed for chlorophorus diadema motschulsky and preparation method for artificial feed |
| KR101222469B1 (en) * | 2012-05-23 | 2013-01-15 | 이대암 | Methods of making artificial diet for callipogon relictus, and mass breeding callipogon relictus using thereof |
| CN103349183A (en) * | 2013-07-17 | 2013-10-16 | 天津拓华生物科技有限责任公司 | Feed for artificially feeding thyestilla gebleri adults and application thereof |
| CN108157688A (en) * | 2017-12-08 | 2018-06-15 | 重庆乾富科技有限公司 | A kind of feed for cultivating chrysanthemum roundheaded borer and preparation method thereof |
| CN113907051B (en) * | 2021-12-03 | 2022-11-04 | 遵义市林业科学研究所 | Artificial breeding method of zanthoxylum bungeanum and tiger longicorn |
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