CN102090377A - Simple method for breeding Apriona germari based on field infestation - Google Patents
Simple method for breeding Apriona germari based on field infestation Download PDFInfo
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- CN102090377A CN102090377A CN2010105993633A CN201010599363A CN102090377A CN 102090377 A CN102090377 A CN 102090377A CN 2010105993633 A CN2010105993633 A CN 2010105993633A CN 201010599363 A CN201010599363 A CN 201010599363A CN 102090377 A CN102090377 A CN 102090377A
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Abstract
The invention belongs to the technical field of insect breeding, in particular relating to a simple method for breeding Apriona germari based on field infestation. The method comprises the following steps: female Apriona germari in the spawning season is inoculated to outdoor paper mulberry branches or poplar branches to lay eggs; the Apriona germari is subjected to repeated infestation; and outdoor live hosts are intensively bred to obtain a great quantity of adults and larva with required state and ages. By utilizing the method, the problems of complex indoor Apriona germari breeding steps, multi-time inoculation, low survival rate and the like can be effectively solved, and a great quantity of test-based adults and larva with consistent physical signs are provided for comparing the field pesticide effect of chemical control.
Description
Technical field
The invention belongs to technical field of insect feeding, be specifically related to trunk borer mulberry longicorn field and connect the worm raising method.
Technical background
Mulberry longicorn (
Apriona germari) genus coleoptera Cerambycidae ditch shin longicorn subfamily.Be distributed in 25 provinces except that Heilungkiang, the Inner Mongol, Jilin, Xinjiang, Ningxia, Tibet in China.Adult, larva all can work the mischief to host plant.Larva is bored moth harm in trunk, influence the height of tree, footpath and growth in volume, reduces lumber quality, even causes trees death.Being the important trunk borer of multiple forest, fruit tree, flowers, also is one of China harm insect the most serious, the most difficult control, the cause harm serious hindrance of economic developments such as becoming China's sericulture, forestry and planting fruit trees industry of its wildness.Research and application that mulberry longicorn effectively prevents and treats method receive much concern.
The mulberry longicorn adult that physical signs is close, the mass rearing of larva are the bases of effectively screening fast, optimization, the control of evaluation mulberry longicorn medicament, technology and other researchs.1 generation of the general 2-3 of mulberry longicorn.The larval phase in 2 years 1 generations, be 22-23 month.The moth of larva food has tangible directionality in the limb, promptly downwards, to trunk moth food, and long average out to 2.77 m of 2 years 1 generations larva burrow, long average out to 3.89 m of 3 years 1 generations larva burrow.These biological propertyes have determined that the indoor feeding of this worm can only be to finish section budding raising of certain worm attitude.The difficult again individuality that obtains a large amount of physical signs unanimities of worm is adopted in the field.Therefore, a large amount of population supplies of mulberry longicorn become the main bottleneck that greatly limits this insect control technical research and popularization.At present, indoor mulberry longicorn is raised the main host of employing juggle method for breeding, phloem method for breeding, artificial feed method for breeding etc., but these methods all exist the loaded down with trivial details need of method repeatedly to transfer, connect problems such as the worm survival rate is low, seriously polluted, and only is suitable for the short-term raising.Mulberry longicorn is raised problem also ubiquity in other stem borer kind insects are raised.
For advancing the breeding technology of stem borer kind insect, reported among the present invention that a kind of mulberry longicorn field connects the worm raising method.This method is utilized its open-air host, and easy and simple to handle, cost is low, and the survival rate height is suitable for the raising of longer cycle, and administrative skill requires also lower, is convenient to obtain the neat adult of physical signs in enormous quantities, larva.
Summary of the invention
The problem that the present invention need solve is to raise for mulberry longicorn to provide a kind of field simple to operate, with low cost, stability and high efficiency to connect the worm method.
Concrete technical scheme of the present invention is as follows:
1) open-air mulberry longicorn adult gathers and raises: in the mulberry longicorn adult eclosion phase, gather the female male imago of sufficient amount, indoor feeding.
2) the lay eggs selection of female adult worm: by the adjusting of indoor feeding time, female worm egg-laying time of egg-laying period was concentrated on after at 3 in afternoon, and select more stable individual standby of day egg laying amount.
3) field connects worm host's preparation: the mulberry longicorn adult is the extra-nutrition host with moraceae plants such as paper mulberry, mulberry treies mainly, the host of laying eggs is mainly willow, paper mulberry, mulberry tree etc., therefore, can select large stretch of wild paper mulberry woods or artificial cultivation poplar forest as meeting the worm host.
4) limb connects the making of worm cage: the branch of mulberry longicorn more options diameter 1-3 cm or limb and close bifurcation are laid eggs.Adopt branch or limb cover cage method to finish the worm work that connects in a large number in field.
1. connect the selection of worm cage material: it is stronger that the mulberry longicorn adult gnaws ability, and the material that connects the worm cage should harder anti-gnawing.
2. connect designing and producing of worm cage: consider paper mulberry or poplar-branch or limb thickness, polypide activity space size, and whether convenient factors such as be installed, will connect the worm cage and be designed to cylindrical shape.
5) field connects the worm technology: install on host's branch and connect the worm cage, insert female worm, and put into 1-2 root paper mulberry branch and get food for it.Nature mulberry longicorn population, every the female worm 3-4 grain of laying eggs every night, all one's life, the common property ovum was about 132.Therefore, can select to adapt to weather condition, use to connect the worm cage, divide 2 groups of fields of hocketing to concentrate in enormous quantities standby female worm and connect worm according to required mulberry longicorn population quantity.
The present invention compared with prior art its beneficial effect is: can utilize the outdoor host plant of mulberry longicorn to concentrate to connect worm and raise, need not switching, the survival rate height can obtain a large amount of required worm attitudes, worm one-tenth, the larva in age, the also field of suitable medicament drug effect screening.
Description of drawings
Fig. 1: design drawing 1. ropes 2. stainless steel window screenings 3. double-deck selvedge 4. slider of zipper fasteners 5. slide fasteners that connect the worm cage.
Fig. 2: the field connects worm cage design sketch.
Embodiment
1. open-air mulberry longicorn adult gathers and raises: 7-8 is when the mulberry longicorn adult sprouts wings in a large number between the month, and the female male imago of field acquisition sufficient amount utilizes water cutting paper mulberry or mulberry branch to raise in indoor (25 ℃, humidity 75%).Male worm is concentrated and raises, and female worm single head is raised.Change fresh branch 1 time every day, note health in the cage.Insert male worm 2-3 time in every female worm cage, finish mating to guarantee female worm.
2. the lay eggs selection of female adult worm: pick out the female worm that post-coitum enters the egg-laying period, single cage is raised.Be fixed on the fresh branch of replacing between 1-3 point in afternoon every day, raised for 1 week continuously.The female worm of the observed and recorded situation of laying eggs every day, and the reaction of changing female worm behind the bar.Change to have behind the bar and obviously sting the groove behavior, and day egg laying amount is more stable, can connect the worm use for the field.
3. the field connects worm host's preparation: the mulberry longicorn adult is the extra-nutrition host with moraceae plants such as paper mulberry, mulberry treies mainly, and the host of laying eggs is mainly willow, paper mulberry, mulberry tree etc., therefore, can select large stretch of wild paper mulberry or artificial cultivation willow as meeting the worm host.The wild paper mulberry aboundresources, but should select to be positioned at the quite more weak paper mulberry of sylvan life, growth potential as meeting the worm host, in order to avoid cause the fragmentation of ovum because of its quick growth.If meet the worm host with the artificial cultivation willow, the willow kind should be based on growth more slowly.
4. limb connects the making of worm cage: adopt branch or limb cover cage method to finish the worm work that connects in a large number in field, connect the worm cage and promptly want convenient disassembly, be suitable for using repeatedly, be convenient for carrying again, and satisfy the lay eggs needs of behavior of female worm.
1. connect the selection of worm cage material: the mulberry longicorn adult has the ability of gnawing more by force, and the material that connects the worm cage should harder anti-gnawing, with metal material for well.Connect the worm cage and need entangle limb, should be light, package back limb and should be positioned at the central authorities of cage, and the shape of cage should be more fixing, can not wave and be out of shape with branch.Consider above factor, finally select for use commercially available stainless steel window screening as connecing the main manufacturing materials of worm cage.Other materials also comprises common slide fastener, cotton bar, rope etc.
2. connect designing and producing of worm cage: the worm cage that connects that is enclosed within on the branch is designed to cylindrical shape.The stainless steel window screening is cut into the rectangle of 40cm * 60cm, a side of on two long limits, same secondary slide fastener being installed respectively, two minor faces seal into double-deck limit with the cotton bar respectively, and penetrate rope therein, the fag end of reservation certain-length.Concrete shape is seen accompanying drawing 1.
5. the field connects the worm technology: choose on the host in the open air connect the worm branch after, rectangle is connect the worm cage is layered on the limb, make long limit parallel with limb.Wherein side minor face branch bifurcation and then around one week of branch, is taken out tight rope, forms the bottom that connects the worm cage.Form the top that connects the worm cage with quadrat method.Then, the opposite side insertion slider of zipper fastener with slide fastener inserts 1-2 female worm, and puts into the fresh branch of 1-2 root and get food for it, has drawn slide fastener.Connect the worm cage and install (seeing accompanying drawing 2).Nature mulberry longicorn population, every the female worm 3-4 grain of laying eggs every night, all one's life, the common property ovum was about 132, and female worm life-span and egg-laying period are all longer.Connecing the worm time can be selected in 7-8 and carry out no rain the time between the month.Standby female worm is divided into 2 groups, all before at 3 in afternoon, received on the host of field for every group, spend the night in the field, second day early 6:30 female worm is fetched indoor feeding, carry out the field every other day again and connect worm.
Claims (1)
1. a mulberry longicorn field connects the simple and easy method that worm is raised, and it is characterized in that being made of following steps:
1, open-air mulberry longicorn adult gathers and raises: 7-8 is when the mulberry longicorn adult sprouts wings in a large number between the month, the female male imago of field acquisition sufficient amount, under indoor 25 ℃, humidity 75% condition, utilize water cutting paper mulberry or mulberry branch to raise, male worm is concentrated and raises, and female worm single head is raised, change fresh branch 1 time every day, note health in the cage, insert male worm 2-3 time in every female worm cage, finish mating to guarantee female worm;
2, the lay eggs selection of female adult worm: pick out the female worm that post-coitum enters the egg-laying period, single cage is raised, be fixed on the fresh branch of replacing between 1-3 point in afternoon every day, raised for 1 week continuously, the female worm of the observed and recorded situation of laying eggs every day, and the reaction of changing female worm behind the bar are changed to have behind the bar and are obviously stung the groove behavior, and day egg laying amount is more stable, can connect worm for the field and use;
3, the field connects worm host's preparation: the mulberry longicorn adult is the extra-nutrition host with moraceae plants such as paper mulberry, mulberry treies mainly, the host of laying eggs is mainly willow, paper mulberry, mulberry tree etc., therefore, can select large stretch of wild paper mulberry or artificial cultivation willow as meeting the worm host, the wild paper mulberry aboundresources, but should select to be positioned at the quite more weak paper mulberry of sylvan life, growth potential as meeting the worm host, in order to avoid cause the fragmentation of ovum because of its quick growth, if meet the worm host with the artificial cultivation willow, the willow kind should be based on growth more slowly;
4, limb connects the making of worm cage: adopt branch or limb cover cage method to finish the worm work that connects in a large number in field, connect the worm cage and promptly want convenient disassembly, be suitable for using repeatedly, be convenient for carrying again, and satisfy the lay eggs needs of behavior of female worm;
1. connect the selection of worm cage material: the mulberry longicorn adult has the ability of gnawing more by force, the material that connects the worm cage should harder anti-gnawing,, connect the worm cage and need entangle limb for well with metal material, should be light, package the central authorities that back limb should be positioned at cage, and the shape of cage should be more fixing, can not wave and be out of shape with branch, considers above factor, finally select for use commercially available stainless steel window screening as connecing the main manufacturing materials of worm cage, other materials also has common slide fastener, cotton bar, rope;
2. connect designing and producing of worm cage: the worm cage that connects that is enclosed within on the branch is designed to cylindrical shape, the stainless steel window screening is cut into the rectangle of 40cm * 60cm, one side of on two long limits, same secondary slide fastener being installed respectively, two minor faces seal into double-deck limit with the cotton bar respectively, and penetrate rope therein, reserve the fag end of certain-length;
5, the field connects the worm method: choose on the host in the open air connect the worm branch after, rectangle is connect the worm cage is layered on the limb, make long limit parallel with limb, wherein side minor face branch bifurcation and then around one week of branch, is taken out tight rope, formation connects the bottom of worm cage, form the top that connects the worm cage with quadrat method, then, with the opposite side insertion slider of zipper fastener of slide fastener, insert 1-2 female worm, and put into the fresh branch of 1-2 root and get food for it, slide fastener connects the worm cage and installs; Nature mulberry longicorn population, every female worm 3-4 grain of laying eggs every night, all one's life, the common property ovum was about 132, female worm life-span and egg-laying period are all longer, connect the worm time can be selected in 7-8 and carry out no rain the time between the month, and standby female worm is divided into 2 groups, every group was all inserted in the cage before at 3 in afternoon, spend the night in the field, second day morning, 6:30 fetched indoor feeding with female worm, carried out the field every other day again and connect worm.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103385221A (en) * | 2013-07-30 | 2013-11-13 | 天津拓华生物科技有限责任公司 | Outdoor artificial feeding method for thyestilla gebleri |
CN105284784A (en) * | 2015-11-26 | 2016-02-03 | 河北省农林科学院植物保护研究所 | Artificial inoculation method of Athetis lepigone |
CN106508813A (en) * | 2016-11-01 | 2017-03-22 | 金华市森林之宝农林科技开发有限公司 | Imitation wild propagation method of mysorethorn moths |
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CN1875744A (en) * | 2006-05-30 | 2006-12-13 | 河南农业大学 | Artificial feed for tubular breeding longhorn beetle larva and breeding method thereof |
CN101228853A (en) * | 2007-01-23 | 2008-07-30 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feeding and culture transfer of monochamus alternatus hope and artificial feed for larva thereof |
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2010
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1875744A (en) * | 2006-05-30 | 2006-12-13 | 河南农业大学 | Artificial feed for tubular breeding longhorn beetle larva and breeding method thereof |
CN101228853A (en) * | 2007-01-23 | 2008-07-30 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feeding and culture transfer of monochamus alternatus hope and artificial feed for larva thereof |
Non-Patent Citations (2)
Title |
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《中国博士学位论文全文数据库农业科技辑》 20080915 金凤 桑天牛产卵分泌物组成、来源和功能的研究 第D049-5页 1 , 第9期 2 * |
《昆虫知识》 19851231 许文华 桑天牛幼虫的人工饲养 第187-189页 1 , 第4期 2 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103385221A (en) * | 2013-07-30 | 2013-11-13 | 天津拓华生物科技有限责任公司 | Outdoor artificial feeding method for thyestilla gebleri |
CN103385221B (en) * | 2013-07-30 | 2015-05-20 | 天津拓华生物科技有限责任公司 | Outdoor artificial feeding method for thyestilla gebleri |
CN105284784A (en) * | 2015-11-26 | 2016-02-03 | 河北省农林科学院植物保护研究所 | Artificial inoculation method of Athetis lepigone |
CN105284784B (en) * | 2015-11-26 | 2017-12-08 | 河北省农林科学院植物保护研究所 | A kind of Methods of Artificial Inoculation of Anoplophora glabripennis of 2 committee noctuid insect |
CN106508813A (en) * | 2016-11-01 | 2017-03-22 | 金华市森林之宝农林科技开发有限公司 | Imitation wild propagation method of mysorethorn moths |
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Application publication date: 20110615 |