CN113907051B - Artificial breeding method of zanthoxylum bungeanum and tiger longicorn - Google Patents
Artificial breeding method of zanthoxylum bungeanum and tiger longicorn Download PDFInfo
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- CN113907051B CN113907051B CN202111462864.1A CN202111462864A CN113907051B CN 113907051 B CN113907051 B CN 113907051B CN 202111462864 A CN202111462864 A CN 202111462864A CN 113907051 B CN113907051 B CN 113907051B
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/195—Antibiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
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Abstract
The invention belongs to the technical field of insect feeding, and particularly relates to an artificial feeding method of zanthoxylum bungeanum and tiger longicorn, which comprises the following steps: s1, collecting pepper tiger longicorn adults; s2, hatching larvae; s3, manufacturing a feeder; s4, preparing a culture medium; s5, throwing in larvae; and S6, feeding management. The invention simulates the growth environment of the zanthoxylum bungeanum after the zanthoxylum bungeanum maxim moths into the zanthoxylum bungeanum branches by artificially manufacturing the feeder and placing the self-made culture medium in the feeder, the method can not only ensure the normal activity and growth of the zanthoxylum bungeanum maxim in the feeder which is very close to the wild environment, and the eating amount (food intake), the growth condition and the shape rule of worm ways of the zanthoxylum bungeanum and the tiger longicorn are convenient to observe, so that a basic guarantee can be effectively provided for the biological control research of the natural enemies of the zanthoxylum bungeanum and the tiger longicorn and the chemical control research of the zanthoxylum bungeanum and the tiger longicorn.
Description
Technical Field
The invention belongs to the technical field of insect feeding, and particularly relates to an artificial feeding method of zanthoxylum bungeanum and tiger longicorn.
Background
Pricklyash peel tiger longhorn beetleClytus valiandus(Fairmaire) belongs to the family Carninaceae of Coleoptera, is an important forest pest on Chinese prickly ash, and both larva and imago of the pest can cause damage to the prickly ash, especially the larva eats the base of a trunk of the prickly ash for more than 5 years and causes huge economic loss due to perennial side branches. The reported hazard rate of zanthoxylum bungeanum and tiger longicorn to zanthoxylum bungeanum in vietnamese county in Sichuan province is as high as 56.35 percent. According to the investigation of the inventor, the hazard rate of the pest in a pricklyash peel park with more extensive management in Guizhou Zunyi is estimated to be as high as 60 percent, and the hazard condition of the pest to pricklyash peel is shown in figure 1. The trunk borers are the most difficult pests to control in forestry in China, and the reasons are as follows: firstly, the insect pest control agent has strong concealment, is not beneficial to observing food taking, insect pest state, age, metamorphosis development, shape rule of insect pest channel, life habit and the like, is difficult to master the optimal control opportunity, and increases the difficulty of control research; in addition, the method is slightly influenced by external environmental factors, provides a relatively superior eating and living environment for the method, and has high propagation speed.
The artificial feeding of the insects has the obvious advantages of reducing the cost of feeding the insects in a large scale, effectively controlling the growth and development uniformity of the insects and the like, and can provide a basic guarantee for the biological control research of the natural enemies of the zanthoxylum bungeanum and the chemical control research of the zanthoxylum bungeanum and the longicorn in a laboratory. Therefore, the development of artificial breeding of zanthoxylum bungeanum and tiger longhorn beetles is very necessary. However, the existing artificial feeding technology for the zanthoxylum bungeanum and the zanthoxylum micranthum longicorn cannot accurately and effectively simulate the growth environment of the zanthoxylum micranthum and the zanthoxylum micranthum after eating the branches and the stems of the zanthoxylum micranthum, so that basic guarantee and data support are difficult to provide for the biological control research on the natural enemies of the zanthoxylum micranthum and the chemical control research on the zanthoxylum micranthum and the longhorn longicorn.
Disclosure of Invention
Aiming at the problems, the invention aims to provide the artificial feeding method of the zanthoxylum bungeanum maxim and the zanthoxylum bungeanum maxim, which can accurately and effectively simulate the growth environment of the zanthoxylum bungeanum maxim after being eaten into the zanthoxylum bungeanum maxim branches, and further can provide basic guarantee for the biological control research of the natural enemies of the zanthoxylum bungeanum maxim and the chemical control research of the zanthoxylum bungeanum maxim.
The purpose of the invention is realized by the following technical scheme:
an artificial feeding method of zanthoxylum bungeanum and tiger longicorn comprises the following steps:
s1, collecting adult zanthoxylum bungeanum and tiger longicorn.
S2, hatching the larvae.
Pairing the captured adult zanthoxylum bungeanum and putting the paired adult zanthoxylum bungeanum into a cage, and feeding the adult zanthoxylum bungeanum by using the zanthoxylum bungeanum branches until the female worms lay eggs and hatch larvae.
S3, manufacturing a feeder:
the feeder comprises a culture cup, a top cover and a light shield;
the culture cup is a hollow thin-wall cylinder with an opening at the top and a closed bottom and made of transparent materials;
the top cover is detachably connected to the opening at the top of the culture cup, and a plurality of air holes are formed in the top cover;
the light shield is covered outside the culture cup and the top cover and is made of breathable light-shielding materials.
S4, preparing a culture medium:
preparing a feed by utilizing a nutritional additive, pepper branch dry powder, pepper branch leaf powder and multivitamin tablet powder, measuring a certain amount of feed according to the volume of a culture cup, and putting the feed into the culture cup to prepare a columnar culture medium.
S5, larva throwing:
uncover the lens hood and open the top cap, chisel out the degree of depth hole that the degree of depth is greater than larva length on the culture medium along the plastics cup inner wall, insert the larva in the degree of depth hole, utilize the culture medium to lightly fill degree of depth hole top, cover the top cap, then at the outer lens hood of culture cup and head cap cover, raise.
S6, feeding management:
and (4) regularly uncovering the light shield, observing and recording the feeding, metamorphosis development and wormhole shape rule of the pepper tiger longhorn beetles in the transparent culture cup, and replacing the culture medium once every 15 days.
Further, the feed preparation steps are as follows:
a. and (4) preparing the nutritional additive.
b. Cutting fresh pepper branches into 5-6cm segments, treating with fresh pepper leaves in an infrared drying oven at 55 deg.C for 48 h, respectively, pulverizing into powder with a pulverizer after completely drying, and sieving with 600 mesh sieve to obtain dry pepper branch powder and pepper branch and leaf powder.
c. Mixing the nutritional additive, fructus Zanthoxyli branch dry powder and fructus Zanthoxyli branch leaf powder, sterilizing in autoclave, cooling to touch state, adding compound vitamin powder, and mixing to obtain feed.
Further, the nutritional additive is prepared by uniformly mixing 1000 mL of distilled water, 50 g of black ant powder, 14 g of tenebrio molitor powder, 10 mL of olive oil, 20g of purified agar powder, 20 mL of fresh egg white, 20 mL of fresh egg yolk, 0.22g of methyl hydroxybenzoate, 0.22g of sorbic acid and 0.22g of roxithromycin; the mass of the pepper branch and leaf powder is 0-100g, and the mass of the vitamin complex tablet powder is 0.5g, wherein the sum of the mass of the pepper branch and leaf powder is 100g.
Furthermore, the mass of the pepper branch dry powder is 80g, and the mass of the pepper branch and leaf powder is 20g.
Further, the light shield is made of breathable black cloth.
Furthermore, the top cover is made of a plastic film with a plurality of air vents, and the outer edge of the top cover is restrained at the top of the culture cup through an elastic belt or a restraining belt.
Further, the culture cup is a 300ml transparent plastic cup with a height of 8-12cm.
Further, the volume of the culture medium in the culture cup is 260ml, and the distance between the top of the culture medium and the cup opening of the culture cup is 1-2cm.
According to the method, the feeder is manufactured artificially, and the self-made culture medium is placed in the feeder to simulate the growth environment of the zanthoxylum bungeanum longicorn after being eaten into the zanthoxylum bungeanum branches, so that the normal activity and growth of the zanthoxylum bungeanum longicorn in the feeder which is very close to the wild environment can be ensured, the eating quality (food intake), the growth condition and the worm way shape rule of the zanthoxylum bungeanum longicorn are conveniently observed, and further, the basic guarantee can be effectively provided for the biological control research of the natural enemies of the zanthoxylum bungeanum longicorn and the chemical control research of the zanthoxylum bungeanum longicorn.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings.
FIG. 1 is a moth-eaten drawing of branches and trunks of pricklyash peel, tiger and longicorn;
FIG. 2 is a diagram showing the comparison between the feeding of Zanthoxylum bungeanum on the branches and the feeding of Zanthoxylum bungeanum in the culture cup, wherein diagrams a and b are diagrams showing the state of feeding on the branches and the feeding of Zanthoxylum bungeanum in the culture cup, and diagrams c and d are diagrams showing the state of feeding in the culture cup.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure. The described embodiments are only some embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be understood that the structures, proportions, and dimensions shown in the drawings and described herein are for illustrative purposes only and are not intended to limit the scope of the present invention, which is defined by the claims, but rather by the claims. In addition, the terms such as "upper", "lower", "left", "right" and "middle" used in the present specification are for convenience of description only, and are not intended to limit the scope of the present invention, and changes or modifications of the relative relationship thereof may be regarded as the scope of the present invention without substantial changes in the technical contents.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1:
an artificial feeding method of zanthoxylum bungeanum and tiger longicorn is characterized by comprising the following steps:
s1, collecting pepper tiger longicorn adults:
collecting and capturing 40 heads of zanthoxylum bungeanum adults from a zanthoxylum bungeanum garden.
S2, hatching larvae.
Selecting 22 healthy adults with consistent individual size from the captured adult zanthoxylum bungeanum, carrying out male-female pairing, putting the adults into an autonomously sewed steel wire mesh insect cage (the length is about 90 cm, the diameter is about 20 cm), continuously collecting fresh zanthoxylum bungeanum branches for supplementing nutrition, and putting coarse branches in the insect cage for spawning. After 2 weeks, after the female adults lay eggs and the larvae are hatched, the branch barks are carefully split, and the initially hatched larvae with the same individual size are collected for testing.
S3, manufacturing a feeder:
the feeder comprises a culture cup, a top cover and a light shield.
The culture cup is a hollow thin-wall cylinder body which is opened at the top and closed at the bottom and is made of transparent materials. The culture cup is a transparent plastic cup (cylindrical shape, simulating the shape of pepper branches) with the volume of 300ml, and the height of the cup is 10cm. A plurality of air outlets can also be arranged on the top of the culture cup near the cup mouth.
The top cover is detachably connected to an opening at the top of the culture cup, and a plurality of air holes are formed in the top cover; the top cover is made of a plastic film with a plurality of air vents, and the outer edge of the top cover is restrained at the top of the culture cup through an elastic belt or a restraining belt.
The light shield is arranged outside the culture cup and the top cover and is made of breathable light-shielding materials, and the light shield is made of breathable black cloth.
S4, preparing a culture medium:
preparing a feed by using a nutritional additive, pepper branch dry powder, pepper branch leaf powder and vitamin complex tablet powder, measuring 260ml of the feed, and putting the feed into a 300ml culture cup to prepare a columnar culture medium. The distance between the top of the culture medium and the cup mouth of the culture cup is 1-2cm.
The preparation steps of the feed are as follows:
a. preparing a nutritional additive:
mixing 1000 mL of distilled water, 50 g of black ant powder, 14 g of tenebrio molitor powder, 10 mL of olive oil, 20g of purified agar powder, 20 mL of fresh egg white, 20 mL of fresh egg yolk, 0.22g of methyl hydroxybenzoate, 0.22g of sorbic acid and 0.22g of roxithromycin uniformly to obtain the nutritional additive.
b. Placing fresh pepper branches and fresh pepper leaves which are cut into 5-6cm segments in an infrared drying oven for processing at 55 ℃ for 48 h, respectively crushing the fresh pepper branches and the fresh pepper leaves into powder by a crusher after the fresh pepper branches and the fresh pepper leaves are completely dried, and sieving the powder by a 600-mesh sieve to prepare pepper branch dry powder and pepper branch leaf powder; the mass of the pepper branch dry powder is 0g, and the mass of the pepper branch and leaf powder is 100g.
c. And (3) fully and uniformly mixing the nutrient additive, the pepper branch dry powder and the pepper branch leaf powder, placing the mixture in an autoclave for sterilization, taking out the mixture, cooling the mixture until the back of the hand is not scalded by touch, adding 0.5g of compound vitamin powder, and uniformly mixing the mixture to prepare the feed.
Namely, the formula of the feed (formula 1 for short) in this embodiment is:
1000 mL of distilled water, 50 g of black ant powder, 14 g of tenebrio molitor powder, 10 mL of olive oil, 20g of purified agar powder, 20 mL of fresh egg white, 20 mL of fresh egg yolk, 0.22g of methyl hydroxybenzoate, 0.22g of sorbic acid, 0.22g of roxithromycin, 0.5g of composite vitamin powder, 0g of pepper branch dry powder and 100g of pepper branch and leaf powder.
S5, larva throwing:
uncover the lens hood and open the top cap, chisel out the degree of depth hole that the degree of depth is greater than larva length on the culture medium along the plastics cup inner wall, insert the larva in the degree of depth hole, utilize the culture medium to lightly fill degree of depth hole top, cover the top cap, then at the outer lens hood of culture cup and head cap cover, raise.
S6, feeding management:
and (5) regularly uncovering the light shield, observing and recording the feeding, metamorphosis development and the shape rule of the wormholes of the pepper tiger longicorn in the transparent culture cup, and replacing the culture medium every 15 days.
Example 2:
this example differs from example 1 in that:
in the feed formula in the step S4, the mass of the pepper branch dry powder is 20g, and the mass of the pepper branch and leaf powder is 80g.
Namely, the formula of the feed (formula 2 for short) in this embodiment is:
1000 mL of distilled water, 50 g of black ant powder, 14 g of tenebrio molitor powder, 10 mL of olive oil, 20g of purified agar powder, 20 mL of fresh egg white, 20 mL of fresh egg yolk, 0.22g of methyl hydroxybenzoate, 0.22g of sorbic acid, 0.22g of roxithromycin, 0.5g of composite vitamin powder, 20g of pepper branch dry powder and 80g of pepper branch and leaf powder.
Example 3:
this example differs from example 1 in that:
in the feed in the step S4, the mass of the used pepper branch dry powder is 40g, and the mass of the pepper branch and leaf powder is 60g.
Namely, the formula of the feed (formula 3 for short) in this embodiment is:
1000 mL of distilled water, 50 g of black ant powder, 14 g of tenebrio molitor powder, 10 mL of olive oil, 20g of purified agar powder, 20 mL of fresh egg white, 20 mL of fresh egg yolk, 0.22g of methyl hydroxybenzoate, 0.22g of sorbic acid, 0.22g of roxithromycin, 0.5g of composite vitamin powder, 40g of pepper branch and leaf powder and 60g of pepper branch and leaf powder.
Example 4:
this example differs from example 1 in that:
in the feed in the step S4, the mass of the pepper branch dry powder is 60g, and the mass of the pepper branch and leaf powder is 40g.
Namely, the formula (formula 4 for short) of the feed in this embodiment is:
1000 mL of distilled water, 50 g of black ant powder, 14 g of tenebrio molitor powder, 10 mL of olive oil, 20g of purified agar powder, 20 mL of fresh egg white, 20 mL of fresh egg yolk, 0.22g of methyl hydroxybenzoate, 0.22g of sorbic acid, 0.22g of roxithromycin, 0.5g of composite vitamin powder, 60g of pepper branch and leaf powder and 40g of pepper branch and leaf powder.
Example 5:
this example differs from example 1 in that:
in the feed in the step S4, the mass of the used pepper branch dry powder is 80g, and the mass of the pepper branch leaf powder is 20g.
Namely, the formula (formula 5 for short) of the feed in this embodiment is:
1000 mL of distilled water, 50 g of black ant powder, 14 g of tenebrio molitor powder, 10 mL of olive oil, 20g of purified agar powder, 20 mL of fresh egg white, 20 mL of fresh egg yolk, 0.22g of methyl hydroxybenzoate, 0.22g of sorbic acid, 0.22g of roxithromycin, 0.5g of composite vitamin powder, 80g of pepper branch dry powder and 20g of pepper branch and leaf powder.
Example 6:
the present example differs from example 1 in that:
in the feed of the step S4, the mass of the used pepper branch dry powder is 100g, and the mass of the pepper branch leaf powder is 0g.
Namely, the formula of the feed (formula 6 for short) in this embodiment is:
1000 mL of distilled water, 50 g of black ant powder, 14 g of tenebrio molitor powder, 10 mL of olive oil, 20g of purified agar powder, 20 mL of fresh egg white, 20 mL of fresh egg yolk, 0.22g of methyl hydroxybenzoate, 0.22g of sorbic acid, 0.22g of roxithromycin, 0.5g of composite vitamin powder, 100g of pepper branch and leaf powder and 0g of pepper branch and leaf powder.
It can be seen that the differences between examples 1 to 6 are mainly due to the different feeding formulations. Wherein the formulation described in example 5 is the optimal formulation.
Fig. 1 shows a worm-eating graph of pepper tree branches and trunks by zanthoxylum bungeanum and horsfoot, and it can be known from the graph that the worm-eating amount (food intake), the growth condition, the shape rule of worm ways, the activity condition and the like are difficult to effectively observe because of being shielded by the pepper branches and trunks, and the pepper branches and trunks need to be cut if the research is needed, which is obviously impossible, so the research on the pepper tree branch and trunk prevention and control technology is not beneficial to the research on the pepper tree and trunk prevention and control technology.
FIG. 2 is a diagram showing the comparison between the feeding of Zanthoxylum bungeanum on the branches and the feeding of Zanthoxylum bungeanum in the culture cup, wherein diagrams a and b are diagrams showing the state of feeding on the branches and the feeding of Zanthoxylum bungeanum, and diagrams c and d are diagrams showing the state of feeding in the culture cup. In the a picture and the b picture, because of being shielded by the pepper branches and trunks, the food intake (food intake), the growth condition, the shape rule of the worm way, the activity condition and the like are difficult to be effectively observed, and the food intake (food intake), the growth condition, the shape rule of the worm way, the activity condition and the like can be effectively observed by adopting the feeding in the transparent culture cup. In order to more accurately master the food intake (food intake), growth condition, wormy shape rule, activity condition and the like of the zanthoxylum bungeanum and tiger longicorn, the artificial feeding is necessary, but the artificial feeding is required to ensure that the artificially simulated growth environment is close to the wild growth environment, the problem is well solved in the embodiment 5 of the application, the feeder and the design of the culture medium imitate the structure of the branches and the stems of zanthoxylum bungeanum, wherein the transparent culture cup can conveniently observe the food intake (food intake), growth condition, wormy shape rule, activity condition and the like of the zanthoxylum bungeanum and tiger longicorn, the components of the culture medium are accurate and effectively simulate the diet of the zanthoxylum bungeanum and tiger, therefore, the wild growth environment of the zanthoxylum bungeanum and tiger can be simulated in a laboratory, the data of the food intake (food intake), growth condition, wormy shape rule, activity condition and the like observed in the laboratory are closer to the data under the wild growth environment, and accurate data support is further provided for the research of the zanthoxylum bungeanum prevention and control technology.
And (3) comparison test:
in order to explore the optimal components of the artificial breeding culture medium of the zanthoxylum bungeanum, the influence of the formula of the culture medium on the survival rate, the pupation rate and the emergence rate of the zanthoxylum bungeanum larvae is explored through a comparison test, the indexes of the survival rate, the pupation rate and the emergence rate are analyzed and compared, and the influence of different branch powder on the development of the zanthoxylum bungeanum larvae is determined.
1. Treatment of test groups:
6 test groups were set up and the 6 test groups were treated as described in examples 1-6, with 15 beetles each, for a total of 3 biological replicates. Treatments for 6 test groups can be considered as treatments for them with different formulations. I.e., the 6 test groups were treated separately using recipes 1-6, one test group for each recipe.
2. Treatment of the control group:
fresh pepper branches (80 cm in length and about 3 cm in diameter at both ends) were inoculated at one end as a control.
Specifically, firstly, the two ends of the prepared fresh branches are sealed by melted solid paraffin wax. Then, a rectangle with only three sides is lightly scribed at a position 15 cm away from the lower end by a knife, the rectangle is lightly uncovered, and the short side of the upper end is reserved. And drilling slightly longer than the insect pore channel by using an electric drill at an inclination angle of 45 degrees, pouring out the wood chips, and inoculating single-head larvae after the pore channel is cooled to room temperature. And finally, covering the rectangular wood part, dipping the melted paraffin by using a brush, brushing the paraffin along the periphery for a circle, and winding the paraffin for 3-4 circles by using a safety film. Each 15 pests are treated, 3 biological repetitions are set, the pests are upwards placed on a sterilized super-clean workbench along the growth direction of the branches, direct sunlight and wind convection are avoided, evaporation of moisture is reduced, an air conditioner, a heater and the like are not started, and the temperature is close to the natural environment temperature as far as possible.
3. Observing and recording survival condition, pupation condition and eclosion condition of zanthoxylum nitidum longicorn larvae respectively
3.1 the effect of the feed formulation on the survival number of larvae is shown in Table 1:
TABLE 1 Effect of feed formulation on survival number of larvae
As can be seen from table 1, the survival rate of the larvae of the zanthoxylum bungeanum longicorn bred by the formula 5 is the closest to that of the control group, i.e. it can be preliminarily considered that the formula and the method described in example 5 are most suitable for breeding the zanthoxylum bungeanum longicorn in the larval stage by simulating the wild environment.
3.2 The influence of the feed formulation on the normal pupation of larvae is shown in Table 2
TABLE 2 influence of feed formulation on Normal pupation of larvae
As can be seen from table 2, the normal pupation rate of the larvae of the zanthoxylum bungeanum longicorn bred by using the formula 5 is the closest to the normal pupation rate of the larvae of the control group, i.e., it can be further considered that the formula and the method described in example 5 are most suitable for breeding zanthoxylum bungeanum longicorn in the pupation period in a wild environment simulation manner.
3.3 The effect of the feed formulation on the normal eclosion of pupae into adults is shown in Table 3
TABLE 3 influence of feed formulation on the normal eclosion of pupae into adults
As can be seen from table 3, the normal pupa emergence rate of the zanthoxylum bungeanum longicorn bred by using the formula 5 is the closest to the normal pupa emergence rate of the control group, i.e. it can be further considered that the formula and the method described in the example 5 are most suitable for simulating the wild environment to breed zanthoxylum bungeanum longicorn in the emergence period.
In conclusion, the formula and the method in the embodiment 5 are most suitable for breeding zanthoxylum bungeanum and tiger longicorn in a simulated wild environment.
Other parts of the invention not described in detail are conventional techniques known to the person skilled in the art.
It should be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The scope of the present invention is not limited to the technical solutions disclosed in the embodiments, and any modifications, equivalent substitutions, improvements, etc. made to the above embodiments according to the technical spirit of the present invention fall within the scope of the present invention.
Claims (6)
1. An artificial feeding method of zanthoxylum bungeanum and tiger longicorn is characterized by comprising the following steps:
s1, collecting pepper tiger longicorn adults:
s2, larva hatching:
pairing the captured adult zanthoxylum bungeanum and putting the paired adult zanthoxylum bungeanum into a cage, and feeding the adult zanthoxylum bungeanum by using the zanthoxylum bungeanum branches until female worms lay eggs and hatch larvae;
s3, manufacturing a feeder:
the feeder comprises a culture cup, a top cover and a light shield;
the culture cup is a hollow thin-wall cylinder body with an opening at the top and a closed bottom and made of transparent materials;
the top cover is detachably connected to an opening at the top of the culture cup, and a plurality of air holes are formed in the top cover;
the light shield is covered outside the culture cup and the top cover and is made of breathable light-shielding materials;
s4, preparing a culture medium:
preparing a feed by utilizing a nutritional additive, pepper branch dry powder, pepper branch leaf powder and multivitamin tablet powder, measuring the feed according to the volume of a culture cup, and putting the feed into the culture cup to prepare a columnar culture medium;
the preparation steps of the feed are as follows:
a. preparing a nutritional additive:
mixing 1000 mL of distilled water, 50 g of black ant powder, 14 g of tenebrio molitor powder, 10 mL of olive oil, 20g of purified agar powder, 20 mL of fresh egg white, 20 mL of fresh egg yolk, 0.22g of methyl hydroxybenzoate, 0.22g of sorbic acid and 0.22g of roxithromycin uniformly to prepare the nutritional additive;
b. placing fresh pepper branches and fresh pepper leaves which are cut into 5-6cm small sections in an infrared drying oven for processing for 48 h at 55 ℃, respectively crushing the fresh pepper branches and the fresh pepper leaves into powder by a crusher after the fresh pepper branches and the fresh pepper leaves are completely dried, and sieving the powder by a 600-mesh sieve to prepare dry pepper branch powder and dry pepper branch and leaf powder; the mass of the pepper branch dry powder is 0-100g, the mass of the pepper branch and leaf powder is 0-100g, and the sum of the mass of the pepper branch dry powder and the mass of the pepper branch and leaf powder is 100g;
c. fully and uniformly mixing the nutritional additive, the pepper branch dry powder and the pepper branch leaf powder, placing the mixture into an autoclave for sterilization, taking out the mixture, cooling the mixture until the back of the hand is not hot, adding 0.5g of compound vitamin powder, and uniformly mixing the mixture to prepare the feed;
s5, larva throwing:
uncovering the light shield and opening the top cover, chiseling a depth hole with the depth larger than the length of the larva on the culture medium along the inner wall of the plastic cup, inserting the larva into the depth hole, filling the top of the depth hole with the culture medium, covering the top cover, and then, covering the light shield outside the culture cup and the top cover for feeding;
s6, feeding management:
the light shield is periodically uncovered, the feeding, metamorphosis development and the shape rule of the wormholes of the pepper tiger longhorn in the transparent culture cup are observed and recorded, and the culture medium is replaced every 15 days.
2. The artificial feeding method of zanthoxylum bungeanum and tiger longicorn as claimed in claim 1, wherein: the mass of the pepper branch dry powder is 80g, and the mass of the pepper branch and leaf powder is 20g.
3. The artificial feeding method of zanthoxylum bungeanum and tiger longicorn as claimed in claim 1, wherein: the light shield is made of breathable black cloth.
4. The artificial feeding method of zanthoxylum bungeanum and tiger longicorn as claimed in claim 1, wherein: the top cover is made of a plastic film with a plurality of air holes, and the outer edge of the top cover is restrained at the top of the culture cup through an elastic belt or a restraining belt.
5. The artificial feeding method of zanthoxylum bungeanum and tiger longicorn according to any one of claims 1 to 4, which is characterized in that: the culture cup is a 300ml transparent plastic cup, and the height of the culture cup is 8-12cm.
6. The artificial feeding method of zanthoxylum bungeanum and tiger longicorn as claimed in claim 5, wherein: the volume of the culture medium in the culture cup is 260ml, and the distance between the top of the culture medium and the cup opening of the culture cup is 1-2cm.
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