CN105918256A - Simple method for artificial feeding of chinese chive maggots - Google Patents
Simple method for artificial feeding of chinese chive maggots Download PDFInfo
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- CN105918256A CN105918256A CN201610169078.5A CN201610169078A CN105918256A CN 105918256 A CN105918256 A CN 105918256A CN 201610169078 A CN201610169078 A CN 201610169078A CN 105918256 A CN105918256 A CN 105918256A
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- 235000018645 Allium odorum Nutrition 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 35
- 244000003377 Allium tuberosum Species 0.000 title abstract 6
- 235000005338 Allium tuberosum Nutrition 0.000 title abstract 6
- 235000006886 Zingiber officinale Nutrition 0.000 claims abstract description 19
- 235000008397 ginger Nutrition 0.000 claims abstract description 19
- 239000007787 solid Substances 0.000 claims abstract description 14
- 244000000383 Allium odorum Species 0.000 claims description 38
- 238000009395 breeding Methods 0.000 claims description 21
- 230000001488 breeding effect Effects 0.000 claims description 21
- 235000013601 eggs Nutrition 0.000 claims description 19
- 241000234314 Zingiber Species 0.000 claims description 18
- 229920001817 Agar Polymers 0.000 claims description 13
- 239000008272 agar Substances 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 10
- 235000013305 food Nutrition 0.000 claims description 7
- 230000012447 hatching Effects 0.000 claims description 6
- 230000001418 larval effect Effects 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 2
- 230000019617 pupation Effects 0.000 abstract description 7
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000011109 contamination Methods 0.000 abstract description 2
- 230000002538 fungal effect Effects 0.000 abstract description 2
- 230000012010 growth Effects 0.000 abstract description 2
- 244000295724 Allium chinense Species 0.000 abstract 1
- 235000016790 Allium chinense Nutrition 0.000 abstract 1
- 240000002234 Allium sativum Species 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 abstract 1
- 244000061456 Solanum tuberosum Species 0.000 abstract 1
- 235000002595 Solanum tuberosum Nutrition 0.000 abstract 1
- 208000033809 Suppuration Diseases 0.000 abstract 1
- 244000273928 Zingiber officinale Species 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 abstract 1
- 230000035558 fertility Effects 0.000 abstract 1
- 235000004611 garlic Nutrition 0.000 abstract 1
- 235000012015 potatoes Nutrition 0.000 abstract 1
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 230000032669 eclosion Effects 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 241000382353 Pupa Species 0.000 description 4
- 230000027326 copulation Effects 0.000 description 4
- 240000008654 Allium ramosum Species 0.000 description 3
- 230000003020 moisturizing effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 230000001932 seasonal effect Effects 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241000208173 Apiaceae Species 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 241001494113 Bradysia Species 0.000 description 1
- 241000613693 Bradysia odoriphaga Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000219104 Cucurbitaceae Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a simple method simple method for artificial feeding of chinese chive maggots, and belongs to the field of insect feeding. The invention uses ginger to replace the traditional artificial feeding, Chinese chives, green Chinese onions, potatoes, garlic and so on. The invention, as compared with a conventional method for feeding chinese chive maggots, has a higher survival rate, pupation rate, emergence rate and fecundity, and saves the cost and labor. In particular, the invention can avoid massive death of the chinese chive maggots or influence on the growth and development thereof caused by suppuration or rot of the above feed due to bacterial or fungal contamination, and can lay a solid foundation for a large number of tests on the chinese chive maggots and provide effective protection.
Description
Technical field
The present invention relates to the simple and easy method of a kind of artificial breeding fragrant-flowered garlic maggot, belong to insecticide and raise field.
Background technology
Fragrant-flowered garlic maggot be Bradysia odoriphaga (Bradysia odoriphagaYang and Zhang) larva, the whole nation all has
Occur, mainly take food 7 section 30 various vegetables and the melon and fruit classes such as Liliaceae, Compositae, Chenopodiaceae, Cruciferae, Cucurbitaceae, Umbelliferae
And edible fungi, especially like Folium Allii tuberosi.Worm polypide is little, reproduction speed is fast, generation overlap is serious for this, be lodged in soil in assemble point
Cloth, difficulty of prevention and cure is big, and general field underproduction 40-60 %, serious field even has no harvest.Therefore, the every research carrying out fragrant-flowered garlic maggot is
Improve the important leverage that Folium Allii tuberosi produces.
Fragrant-flowered garlic maggot is as research and controlling object, and worm source is essential.Therefore, large scale artificial breeding's technology of fragrant-flowered garlic maggot is to carry out
The basic premise of fragrant-flowered garlic maggot research.But, the existing technology raising fragrant-flowered garlic maggot mainly includes man-made feeds, Folium Allii tuberosi, Herba Alii fistulosi etc., the most easily
Because of antibacterial or fungal contamination, cause feedstuff pus or rot, cause fragrant-flowered garlic maggot mortality or affect its growth promoter.It addition, it is existing
There is the feedstuff in breeding technology all to be changed by seasonal variations, do not have seriality.Accordingly, it is possible to exist because worm source difference causes
Experimental error or inaccuracy.Meanwhile, breeding technology adds a large amount of cost of labor, especially fragrant-flowered garlic maggot transfer when feedstuff is changed
Waste time and energy, and feedstuff adds various composition and adds the cost of feedstuff.
Summary of the invention
For many deficiencies present in existing fragrant-flowered garlic maggot method for breeding, technical problem solved by the invention is to provide one
The simple and easy method of artificial breeding fragrant-flowered garlic maggot, the fresh fragrant-flowered garlic during long-term visible Rhizoma Zingiberis Recens replaces existing method for breeding on the method market
Dish, Herba Alii fistulosi and man-made feeds etc., the fragrant-flowered garlic maggot raised by the inventive method has higher survival rate, percentage of pupation, eclosion rate and product
Ovum rate, and quality is homogeneous and stable, provides worm source to ensure for carrying out the research of fragrant-flowered garlic maggot.
Present invention provide the technical scheme that the simple and easy method of a kind of artificial breeding fragrant-flowered garlic maggot, comprise the steps:
(1) feedstuff processes: Rhizoma Zingiberis Recens is cut into thread or lamellar；
(2) select clean culture dish, within it spread solid agar；
(3) take caliber size upper cover consistent with culture dish, tip upside down on culture dish, with sealed membrane, connector is sealed；
(4) again at upper cover side opening osculum, by osculum, female male imago is proceeded to, be placed in incubator, allow its mate and oviposit；
(5) wait to lay eggs complete, after adult death, take out culture dish, remove upper cover, then the shredded ginger cut is placed on pieces of an egg side, lid
Upper culture dish lid, is placed in incubator；
(6) after egg hatching, larval feeding shredded ginger, after shredded ginger has taken food, in culture dish, put into Rhizoma Zingiberis the most again, eat for it
With；
(6) until pupating, treating that fragrant-flowered garlic maggot turns into adult, then adult is shifted, repeating above-mentioned incubation.
In order to ensure the suitable humiture that fragrant-flowered garlic maggot grows, it is preferable that above-mentioned raising is all 25 ± 1 in temperatureoC is the wettest
Degree is 70 ± 2%, carries out in the incubator of photoperiod L:D=12:12；Solid agar concentration in step (2) is 2.5%
The simple and easy method of artificial breeding fragrant-flowered garlic maggot of the present invention, the most preferably, specifically includes following steps:
(1) feedstuff processes: after Rhizoma Zingiberis Recens is bought back, and cleans airing, cuts the thick thread or lamellar of 2-3mm；
(2) the clean culture dish selecting height to be 1.5cm, the solid agar of layer overlay 0.3-0.5cm within it, plays moisturizing and makees
With；
(3) plastic cup is pruned so that it is caliber size is consistent with culture dish, tip upside down on culture dish, will be even with sealed membrane
Interface seals, and prevents cup from departing from culture dish；
(4) again at plastic cup side opening osculum, by osculum, female male imago is proceeded to, be placed in incubator, allow its copulation produce
Ovum；
(5) wait to lay eggs complete, after adult death, take out culture dish, remove sealed membrane, take off plastic cup, then the shredded ginger that will cut
It is placed on pieces of an egg side, covers culture dish lid, be placed in incubator；
(6) after egg hatching, larval feeding shredded ginger, after shredded ginger has taken food, in culture dish, put into Rhizoma Zingiberis the most again, eat for it
With, until pupating, treating that fragrant-flowered garlic maggot turns into adult, then adult is transferred to the new culture dish with back-off plastic cup and solid agar
In, repeat above step and continue to cultivate.
The method have the advantages that the research of fragrant-flowered garlic maggot harm Rhizoma Zingiberis Recens has no report, but inventor is by Folium Allii tuberosi, Bulbus Allii, ocean
Herba Alii fistulosi and Rhizoma Zingiberis Recens are put in same culture dish, do selectivity experiment, found that fragrant-flowered garlic maggot is close to the selection rate of Rhizoma Zingiberis Recens and Folium Allii tuberosi, more individually
When raising fragrant-flowered garlic maggot with Rhizoma Zingiberis Recens, found that fragrant-flowered garlic maggot can grow by normal growth, therefore, it is entered detailed research.The present invention overcomes
Because seasonal variations causes feedstuff nervous, it is achieved that serialization is raised, and there is the advantages such as quality is homogeneous, stable, it is possible to guarantee
The concordance of Jiu Quchongyuan, accuracy and preciseness for experiment provide powerful guarantee.
Jiang Hanyou rich in protein in the present invention, saccharide, vitamin etc., and containing plant antibiotic, not only nutrition
Abundant, fragrant-flowered garlic maggot larval growth can be met and grow required；And the pollution of various mushroom can be resisted, it is ensured that its quality.
In sum, after using method for breeding of the present invention, homogeneity and the stability of fragrant-flowered garlic maggot quality have had bigger
Raising, and there is higher survival rate, percentage of pupation, eclosion rate and spawning rate, a large amount of for fragrant-flowered garlic maggot taking a firm foundation for examinations,
Powerful guarantee is provided.
Detailed description of the invention
It is further elucidated with the present invention below by the detailed description of detailed description of the invention, but is not the limit to the present invention
System, only illustrates.
Embodiment 1
The simple and easy method of a kind of artificial breeding fragrant-flowered garlic maggot, specifically includes following steps:
(1) feedstuff processes: after Rhizoma Zingiberis Recens is bought back, and cleans airing, cuts the thick thread or lamellar of 2-3mm；
(2) the clean culture dish selecting height to be 1.5cm, the solid agar of layer overlay 0.3-0.5cm within it, rise and protect
Wet effect；
(3) plastic cup is pruned so that it is caliber size is consistent with culture dish, tip upside down on culture dish, will be even with sealed membrane
Interface seals, and prevents cup from departing from culture dish；
(4) again at plastic cup side opening osculum, by osculum, female male imago is proceeded to, be placed in incubator, allow its copulation produce
Ovum；
(5) wait to lay eggs complete, after adult death, take out culture dish, remove sealed membrane, take off plastic cup, then the shredded ginger that will cut
It is placed on pieces of an egg side, covers culture dish lid, be placed in incubator；
(6) after egg hatching, larval feeding shredded ginger, after shredded ginger has taken food, in culture dish, put into Rhizoma Zingiberis the most again, eat for it
With.Until pupating, treating that fragrant-flowered garlic maggot turns into adult, then adult is transferred to the new culture dish with back-off plastic cup and solid agar
In, repeat above step and continue to cultivate.
Described cultivation the temperature inside the box is 25 ± 1 DEG C, and relative humidity is 70 ± 2%, photoperiod L:D=12:12.
Embodiment 2
The simple and easy method of a kind of artificial breeding fragrant-flowered garlic maggot, specifically includes following steps:
(1) feedstuff processes: after Rhizoma Zingiberis Recens is bought back, and cleans airing, cuts the thick thread or lamellar of 2-3mm；
(2) the clean culture dish selecting height to be 1.5cm, the solid agar of layer overlay 0.3-0.5cm within it, plays moisturizing and makees
With；
(3) plastic cup is pruned so that it is caliber size is consistent with culture dish, tip upside down on culture dish, will be even with sealed membrane
Interface seals, and prevents cup from departing from culture dish；
(4) again at plastic cup side opening osculum, by osculum, female male imago is proceeded to, be placed in incubator, allow its copulation produce
Ovum；
(5) wait to lay eggs complete, after adult death, take out culture dish, remove sealed membrane, take off plastic cup, then the shredded ginger that will cut
It is placed on pieces of an egg side, covers culture dish lid, be placed in incubator；
(6) after egg hatching, larval feeding shredded ginger, after shredded ginger has taken food, in culture dish, put into Rhizoma Zingiberis the most again, eat for it
With.Until pupating, treating that fragrant-flowered garlic maggot turns into adult, then adult is transferred to the new culture dish with back-off plastic cup and solid agar
In, repeat above step and continue to cultivate.
Described cultivation the temperature inside the box is 25 ± 1 DEG C, and relative humidity is 60 ± 2%, photoperiod L:D=10:14.
Embodiment 3
The simple and easy method of a kind of artificial breeding fragrant-flowered garlic maggot, specifically includes following steps:
(1) feedstuff processes: after Rhizoma Zingiberis Recens is bought back, and cleans airing, cuts the thick thread or lamellar of 2-3mm；
(2) the clean culture dish selecting height to be 1.5cm, the solid agar of layer overlay 0.3-0.5cm within it, plays moisturizing and makees
With；
(3) plastic cup is pruned so that it is caliber size is consistent with culture dish, tip upside down on culture dish, will be even with sealed membrane
Interface seals, and prevents cup from departing from culture dish；
(4) again at plastic cup side opening osculum, by osculum, female male imago is proceeded to, be placed in incubator, allow its copulation produce
Ovum；
(5) wait to lay eggs complete, after adult death, take out culture dish, remove sealed membrane, take off plastic cup, then the shredded ginger that will cut
It is placed on pieces of an egg side, covers culture dish lid, be placed in incubator；
(6) after egg hatching, larval feeding shredded ginger, after shredded ginger has taken food, in culture dish, put into Rhizoma Zingiberis the most again, eat for it
With.Until pupating, treating that fragrant-flowered garlic maggot turns into adult, then adult is transferred to the new culture dish with back-off plastic cup and solid agar
In, repeat above step and continue to cultivate.
Described cultivation the temperature inside the box is 25 ± 1 DEG C, and relative humidity is 80 ± 2%, photoperiod L:D=14:10.
Comparative example 1
Under the same conditions, man-made feeds (preparing with reference to the method in ZL200810238713.6), Folium Allii tuberosi cauloid, Rhizoma Solani tuber osi are used
Fragrant-flowered garlic maggot is raised, the statistics survival rate of fragrant-flowered garlic maggot, percentage of pupation, pupa substance, eclosion rate and single the most female lay eggs with the method described in embodiment 1
Amount, result is as follows:
As can be seen from the above results: use embodiment 1 method to raise fragrant-flowered garlic maggot, there is higher survival rate, percentage of pupation, pupa list
Weight, eclosion rate and single female egg laying amount.Wherein, fragrant-flowered garlic maggot survival rate a little higher than Folium Allii tuberosi cauloid and the people of the raising of embodiment 1 method are used
The fragrant-flowered garlic maggot of work diet, but there is no significant difference；Percentage of pupation, average pupa weight, eclosion rate and single female egg laying amount closely fragrant-flowered garlic
The percentage of pupation of dish raising and average pupa weight；And the most a little higher than man-made feeds.Meanwhile, utilize Jiang Zuowei feedstuff, saving of work and time, save
Cost, is a kind of recommendable good method.
Claims (7)
1. the simple and easy method of an artificial breeding fragrant-flowered garlic maggot, it is characterised in that: specifically include following steps:
(1) feedstuff processes: Rhizoma Zingiberis Recens is cut into thread or lamellar；
(2) select clean culture dish, within it spread solid agar；
(3) take caliber size upper cover consistent with culture dish, tip upside down on culture dish, with sealed membrane, connector is sealed；
(4) again at upper cover side opening osculum, by osculum, female male imago is proceeded to, be placed in incubator, allow its mate and oviposit；
(5) wait to lay eggs complete, after adult death, take out culture dish, remove upper cover, then the shredded ginger cut is placed on pieces of an egg side, lid
Upper culture dish lid, is placed in incubator；
(6) after egg hatching, larval feeding shredded ginger, after shredded ginger has taken food, in culture dish, put into Rhizoma Zingiberis the most again, eat for it
With；
(6) until pupating, treating that fragrant-flowered garlic maggot turns into adult, then adult is shifted, repeating above-mentioned incubation.
2. according to the method for breeding described in claim 1, it is characterised in that: the solid agar concentration in described step (2)
It is 2.5%.
3. according to the method for breeding described in claim 1, it is characterised in that: described cultivation the temperature inside the box is 25 ± 1 DEG C,
Relative humidity is 70 ± 2%, photoperiod L:D=12:12.
4. according to the method for breeding described in claim 1, it is characterised in that: Rhizoma Zingiberis Recens is cut into the thick silk of 2-3mm by step (1)
Shape or lamellar.
5. according to the method for breeding described in claim 1, it is characterised in that: described upper cover is to be pruned by plastic cup, makes
Its caliber size is consistent with culture dish.
6. according to the method for breeding described in claim 1, it is characterised in that: the culture dish height chosen is 1.5cm.
7. according to the method for breeding described in claim 1, it is characterised in that: step (2) is spread the thickness of solid agar
0.3-0.5cm。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610169078.5A CN105918256B (en) | 2016-03-23 | 2016-03-23 | A kind of simple and easy method of artificial feeding bradysia odoriphaga |
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| CN201610169078.5A CN105918256B (en) | 2016-03-23 | 2016-03-23 | A kind of simple and easy method of artificial feeding bradysia odoriphaga |
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Cited By (2)
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| CN107173336A (en) * | 2017-07-14 | 2017-09-19 | 山东省农业科学院植物保护研究所 | A kind of fragrant-flowered garlic maggot method for breeding |
| CN107736307A (en) * | 2017-10-24 | 2018-02-27 | 河北省农林科学院植物保护研究所 | A kind of method that fungus gnat is raised using sweet potato |
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| CN107173336A (en) * | 2017-07-14 | 2017-09-19 | 山东省农业科学院植物保护研究所 | A kind of fragrant-flowered garlic maggot method for breeding |
| CN107736307A (en) * | 2017-10-24 | 2018-02-27 | 河北省农林科学院植物保护研究所 | A kind of method that fungus gnat is raised using sweet potato |
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| Publication number | Publication date |
|---|---|
| CN105918256B (en) | 2019-03-29 |
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