KR100252387B1 - A method for breeding psacothea hilaris - Google Patents
A method for breeding psacothea hilaris Download PDFInfo
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- KR100252387B1 KR100252387B1 KR1019970063550A KR19970063550A KR100252387B1 KR 100252387 B1 KR100252387 B1 KR 100252387B1 KR 1019970063550 A KR1019970063550 A KR 1019970063550A KR 19970063550 A KR19970063550 A KR 19970063550A KR 100252387 B1 KR100252387 B1 KR 100252387B1
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000009395 breeding Methods 0.000 title claims description 8
- 230000001488 breeding effect Effects 0.000 title claims description 7
- 241000027515 Psacothea hilaris Species 0.000 title abstract 2
- 240000000249 Morus alba Species 0.000 claims abstract description 22
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 22
- 239000000843 powder Substances 0.000 claims abstract description 18
- 229940113118 carrageenan Drugs 0.000 claims abstract description 4
- 235000010418 carrageenan Nutrition 0.000 claims abstract description 4
- 229920001525 carrageenan Polymers 0.000 claims abstract description 4
- 239000000679 carrageenan Substances 0.000 claims abstract description 4
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 claims abstract description 4
- 241000283690 Bos taurus Species 0.000 claims description 21
- 235000013601 eggs Nutrition 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 244000061458 Solanum melongena Species 0.000 claims description 5
- 235000002597 Solanum melongena Nutrition 0.000 claims description 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 4
- 230000003020 moisturizing effect Effects 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 229960005091 chloramphenicol Drugs 0.000 claims description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 claims description 2
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 2
- 235000010199 sorbic acid Nutrition 0.000 claims description 2
- 239000004334 sorbic acid Substances 0.000 claims description 2
- 229940075582 sorbic acid Drugs 0.000 claims description 2
- 241001116389 Aloe Species 0.000 claims 1
- 235000011399 aloe vera Nutrition 0.000 claims 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims 1
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims 1
- 229960002216 methylparaben Drugs 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- 229920003002 synthetic resin Polymers 0.000 claims 1
- 239000000057 synthetic resin Substances 0.000 claims 1
- 241000255789 Bombyx mori Species 0.000 abstract description 4
- 230000012447 hatching Effects 0.000 abstract description 4
- 241000238631 Hexapoda Species 0.000 description 8
- 241000894007 species Species 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 241000254173 Coleoptera Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 241000382353 Pupa Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
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- 229940121375 antifungal agent Drugs 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 235000021185 dessert Nutrition 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000021137 habitual diet Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- -1 methyl parahydroxybenzoic acid Chemical compound 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
Description
본 발명은 희귀종 곤충인 울도하늘소 인공 증식방법 및 그 증식용 인공사료에 관한 것으로, 특히 휴면기를 타파하여 연중 대량으로 증식시킬 수 있는 방법 그에 적합한 인공사료에 관한 것이다.The present invention relates to an artificial breeding method of the Uldo Sky cattle, which is a rare insect, and an artificial feed for propagating the same, and more particularly, to a method capable of proliferating in a large amount throughout the year by dormant phase and an artificial feed suitable therefor.
최근 국내 자연환경등에 의한 생태계의 파괴로 인하여 곤충을 비롯한 유용한 동식물의 종이 점차 멸종되어 가고 있으며, 이러한 희귀종 또는 멸종위기의 동식물에 대하여 종의 보존 및 자원화 차원에서 보존의 필요성이 국내외에서 인식되어 멸종위기의 동식물 보존을 위한 연구가 활발하게 이루어지고 있다. 이와같이 사라져가고 있는 유용한 곤충의 하나인 딱정벌레목 곤충인 울도하늘소도 오래전부터, 80년대 이후에 채집한 기록이 없어 멸종위기에 놓여 있으며, 딱정벌레 곤충 중에서 세대가 짧고 휴면타파에 의해 연중 대량증식이 가능한 유일한 종으로서 딱정벌레에 대한 연구를 위한 실험곤충으로 이용할 수 있을 뿐만 아니라 성충 수명이 40여일로 길며, 촉각이 길고 아름다워 자연학습교재용 혹은 애완곤충으로 이용될 수 있어 이의 보존을 위하여 증식방법이 요구되어 왔다.Recently, due to the destruction of the ecosystem by the natural environment in Korea, useful species of animals and plants are gradually extinct, and the necessity of preservation of these rare species or endangered animals and plants in domestic and foreign countries is recognized as endangered species. Research on the preservation of animals and plants is being actively conducted. One of these useful vanishing insects, the coleopteran insect, Uldo Sky Cow, has long been extinct due to the lack of records collected since the 80's, and the shortest generation of beetle insects and capable of mass growth throughout the year by dormant breakouts. As a species, not only can be used as an experimental insect for the study of the beetle, but the adult lifespan is long as about 40 days, and the sense of touch is long and beautiful, so it can be used as a natural learning textbook or as a pet insect, and a growth method has been required for its preservation.
이에 따라, 울도하늘소의 인공증식이 일본에서 시도되었으며 그 증식방법을 참고로 기재하면, 먼저 산란한 난이 부착된 뽕나무가지에서 알을 수거하여 부화시키고 노숙 유충이 되면 5℃에서 40일간 냉장하여 휴면기를 타파하여 년중 계대사육이 가능하도록 하고 있다. 또한, 사육에 필요한 인공사료는 누에용 인공 사료분말 100부와 일반 펌프분말 100부, 성형제로서 한편 10부를 혼합하여 미량의 방부재와 항생재를 첨가한 물로 반죽한 다음 분쇄한 것으로서 셀룰로오즈원으로 일반 펄프분말을 사용하는데 특징이 있다.Accordingly, artificial breeding of Uldo Sky Cow was attempted in Japan, and the method of growth is described by reference. First, eggs are collected from hatched mulberry branches with eggs laid and hatched, and when they are homeless larvae, they are refrigerated at 5 ° C. for 40 days and are dormant. It is possible to breed subsidiaries during the year. In addition, the artificial feed necessary for breeding is 100 parts of artificial feed powder for silkworms, 100 parts of general pump powder, and 10 parts as a molding agent, which is kneaded with water added with a small amount of antifungal and antibiotics, and then pulverized. It is characterized by the use of powder.
그러나, 울도하늘소는 년 1세대 발생으로 휴면기를 갖고 있어 대량 사육에 시간이 많이 소요되고, 성충의 교미, 산란 및 알 수거에 많은 인력이 필요하게 되는 어려움과, 실내에서의 대량증식을 위한 실내적응성이 낮아 부화율 및 성충화율이 낮고 증식환경이 유지에 어려움이 많았으며, 인공사료의 비용이 많이 소요되고 충체의 활력이 저하되어 병원균에 대한 저항력이 저하되는 문제가 많았다.However, Uldo Sky Cattle has a dormant period due to the 1st generation of the year, which takes much time for mass breeding, requires a lot of manpower for mating, spawning, and collecting eggs, and adaptability for mass breeding indoors. The low incubation rate and low adultization rate, the proliferation environment was difficult to maintain, the cost of artificial feed was high, the vitality of the charge was lowered and the resistance to pathogens was lowered.
본 발명은 상술한 종래 울도하늘소의 영구보존을 위한 대략증식에 따른 문제를 해결하기 위한 것으로, 울도하늘소의 휴면기를 타파하고 인공사료를 공급함으로써 일년 내내 대량 증식할 수 있는 증식방법의 제공을 목적으로 한다.The present invention is to solve the problem according to the approximate growth for the permanent preservation of the above-mentioned conventional Uldo sky cow, and to provide a proliferation method that can proliferate throughout the year by breaking through the dormant period of Uldo sky cow and supplying artificial feed. do.
제1도는 본 발명에 따른 울도하늘소 증식방법을 도시한 블록도.Figure 1 is a block diagram showing a Uldo sky cattle growth method according to the present invention.
상기한 목적을 달성하기 위한 본 발명의 울도하늘소 대량 증식방법은 산란가지에서 난을 부하시킨 후 유충을 수거하고, 수거된 유충을 적어도 25℃의 고온에서 14~16시간 정도 조명하의 고온장일조건으로 애누에용 사료에 뽕나무가지 분말을 포함한 인공사료를 공급하는 증식환경하에서 수대에 걸쳐 계대증식하여 휴면이 타파되도록 하는 것으로 구성된다.In order to achieve the above object, the method of mass-proliferation of Uldo sky cattle of the present invention collects the larvae after loading the eggs from the eggplant branch, and collects the collected larvae under high temperature and working conditions under illumination for about 16 to 16 hours at a high temperature of at least 25 ° C. It is composed of several generations of subcultures under the proliferation environment that supplies artificial feed including mulberry eggplant powder to silkworm feed to break dormancy.
이하, 본 발명의 실시예를 도시한 첨부도면을 참조하여 본 발명을 상세히 설명하기로 한다.Hereinafter, with reference to the accompanying drawings showing an embodiment of the present invention will be described in detail the present invention.
본 발명의 울도하늘소 증식방법은 제1도에 블록도로 도시된 바와 같이 뽕나무가지에 산란된 난을 보호하는 난 보호 단계와 증식단계로 구분되고, 난보호 단계에서는 산란된 난을 수거하지 않고 7일동안 25℃로 유지하여 보호한다. 이를 위하여 울도하늘소가 산란하기 쉽게 적정 크기로 자란 뽕나무 가지에 알을 산란하도록 하는 것이 바람직하다.Uldo sky cattle growth method of the present invention is divided into an egg protection step and a propagation step to protect the eggs scattered on the mulberry branches as shown in the block diagram in FIG. 1, in the egg protection step 7 days without collecting the scattered eggs Protect at 25 ° C for For this purpose, it is preferable to spawn eggs on mulberry branches grown to an appropriate size so that the Uldo Sky Cow can be easily spawned.
증식단계는 도면에 도시된 바와 같이 난으로부터 부하된 유충들을 수거하여 노숙유충이 되기까지 55일동안 개별적으로 샬레와 같은 용기에서 개체증식을 하며, 이때도 역시 25℃로 고온상태로 유지한다. 개체증식기간에는 본 발명에 따라 조성된 인공사료를 공급하되 노숙유충직전까지 사료를 공급하며, 노숙유충기에는 사료를 먹지 않으므로 보습만 시행하고 일정기간을 두고 용기를 세척, 소독하여 교환함으로써 청결을 유지하여 유충이 질병에 걸리지 않도록 하고, 보습은 물에 적신 종이를 용기에 넣어 습도가 일정하게 유지되게 함으로써 수행한다.The propagation step collects the larvae loaded from the eggs as shown in the drawing and individually propagates in a container such as a chalet for 55 days until it becomes a homeless larva, and is also maintained at 25 ° C. at a high temperature. In the individual growth period, the artificial feed formulated according to the present invention is supplied, but the feed is provided immediately before the homeless larvae, and the homeless larvae do not eat the food, so that only moisturizing is performed and the container is washed, disinfected and exchanged for a certain period of time to keep clean The larvae do not get sick, and moisturizing is carried out by putting a paper moistened with water in a container to keep the humidity constant.
노숙유충기를 거친 유충은 번데기로 용화하며, 용화단계의 13일 동안에는 25℃하에서 보습만 시행한다.The larvae that go through the homeless larvae are lysed by the pupa and only moisturize at 25 ° C during the 13 days of the larvae stage.
번데기 상태의 용화기간을 지난 다음 번데기가 우화하여 성충이 되며, 우화된 성충은 처음 3~4일 동안의 후식기에 인공사료를 공급하여 완전한 성충으로 성장한다. 상기 보습은 물에 적신 종이를 용기에 넣어 수행한다.After the pupae period of pupation, the pupa becomes an adult and becomes an adult, and the adult adult grows into a full adult by supplying artificial feed to the dessert for the first 3-4 days. The moisturizing is performed by putting a paper moistened with water in a container.
이와 같이 증식된 울도하늘소 성충은 뽕나무가지에서 교미하도록 하며, 울도하늘소의 알이 산란된 뽕나무 가지를 채취하여 저장하여 상술한 바와 같은 증식단계들을 반복하여 수행함으로써 대량 증식이 가능하다. 또한, 뽕나무가지에 산란된 알이 산란된 상태 그대로 유지되므로 부화율이 향상되고 종래와 같이 알 수거에 따른 인력손실의 문제가 제거된다.Thus, the adult Uldo sky cattle adult to be mated in the mulberry branches, by collecting and storing the mulberry branches scattered eggs of the Uldo sky cow can be carried out by repeating the growth steps as described above, mass growth is possible. In addition, since the eggs scattered on the mulberry branch is maintained in the scattered state, the hatching rate is improved and the problem of attraction loss due to harvesting eggs is eliminated as in the prior art.
본 발명에 따른 울도하늘소 증식용 인공사료는 기존의 누에용 인공사료분말, 예를 들어 잠시형 4호 애누에용 인공사료 100부에, 뽕나무가지분말 100부, 성형제로서 카라기낭 10부를 혼합하여 조성된 것이다. 상기 뽕가지분말은 뽕가지를 수확하여 겉껍질을 제거하고 60℃에서 수분을 3% 이하로 가열건조한 다음 파쇄기에서 파쇄하여 분말화하고 그 분말중에서 50~80메시의 분말을 선별한 것이다. 상기와 같이 조성된 분말상의 인공사료 혼합물을 물 300ml에 클로람페니콜 100mg, 메칠파라하이드록시 안식향산 750mg, 소르빈산 500mg, 포르말린 1ml를 잘 섞은 것에 첨가하여 반복한 다음 고압멸균기에 넣고 105℃에서 10분간 가열한 후 꺼내어 다시 혼합하여 합성수지 필름으로 덮고 찬물에 냉각한 다음 저장하여 필요량씩 사용한다.Uldo Sky cattle breeding artificial feed according to the present invention by mixing 100 parts artificial artificial feed for silkworms, for example, 100 parts artificial fodder for Hangul No. 4, 100 parts of mulberry branch powder, 10 parts of carrageenan as a molding agent It was created. The mulberry branch powder is harvested mulberry branch to remove the outer shell, heat-dried to less than 3% moisture at 60 ℃ and then crushed in a crusher to powder and selected from 50 to 80 mesh powder from the powder. The powdered artificial feed mixture prepared as described above was added to 300 ml of water in 100 mg of chloramphenicol, 750 mg of methyl parahydroxybenzoic acid, 500 mg of sorbic acid, and 1 ml of formalin. Take out, mix again, cover with synthetic film, cool in cold water, store and use as needed.
실제로 울도하늘소의 증식에서 동일 증식조건하에 전술한 일본에서 개발된 인공사료를 사용한 경우와 본 발명에 의한 인공사료를 사용한 경우를 비교한 결과, 실제로 울도하늘소의 증식에서 동일 증식조건하에 전술한 일본에서 개발된 인공사료를 사용한 경우와 본 발명에 의한 인공사료를 사용한 경우를 비교한 결과, 우화율에 있어서 일본 사료를 사용한 경우에는 62%로 나타났으나, 본 발명에 의한 인공사료를 사용한 경우에는 67.5%로 나타나 유충생존율이 5.5%이상 향상되는 것으로 관찰되었다.In fact, the comparison between the case of using the artificial feed developed in Japan and the case of using the artificial feed according to the present invention under the same propagation conditions in the growth of the Uldo Sky cattle was carried out in the above-mentioned Japan under the same growth conditions in the growth of the Uldo Sky Cattle. Comparing the case of using the developed artificial feed with the artificial feed according to the present invention, the allegory rate was 62% when using the Japanese feed, but when using the artificial feed according to the present invention, 67.5 The larval survival rate was improved by more than 5.5%.
이러한 결과는 본 발명에 따라 울도하늘소 증식시 공급되는 인공사료는 울도하늘소의 먹이 식물인 뽕나무가지를 포함하여, 본 발명의 인공사료에 의해 증식되는 유충의 경우 사료에 대한 적응성이 높아 총체가 튼튼하고 세대가 반복됨에 따라 생물활력의 감소가 없고 병원균에 대한 저항성이 높아지기 때문인 것으로 분석되었으며, 따라서 성충화율도 향상되는 것으로 나타났다.These results indicate that the artificial feed supplied when the Uldo Sky Cow is grown according to the present invention includes mulberry branches, which are food plants of the Uldo Sky Cow, and in the case of the larva that is grown by the artificial feed of the present invention, the adaptability to the feed is high, and the total is strong. As the generation is repeated, it is analyzed that there is no decrease in bioactivity and resistance to pathogens is increased.
본 발명에 따라, 뽕나무가지 표피속에 산란된 울도하늘소의 알을 수거하지 않고 부화된 유충을 수거함으로써 인력의 절감과 부화율이 향상되고, 증식시 울도하늘소의 거주식인 뽕나무가지분말을 함유한 인공사료를 공급함으로써 사료비를 절감할 수 있으며 성충화율이 향상되고 생물활력이 증가되어 울도하늘소를 멸종위기로부터 인공증식에 의해 유용곤충자원으로 영구보존할 수 있을 뿐만 아니라, 딱정벌레목의 곤충연구에 이용하여 증식기술의 체계화를 가능하게 하고 학생들의 자연학습교재로서 및 애완곤충으로 상품화될 수 있도록 하는 효과가 있다.According to the present invention, by collecting hatched larvae without collecting eggs of Uldo sky cows scattered in the skin of mulberry branches, the reduction of manpower and hatching rate is improved, and artificial feed containing mulberry eggplant powder, which is a habitual diet of Uldo sky cows, is grown. Feeding can reduce feed costs, improve adult fertility rate, and increase biological viability, so that not only can the Uldo Sky Cow be permanently stored as a useful insect resource by artificial propagation from endangered species, but also it can be used for insect research of coleoptera. It has the effect of enabling the systematization of students and commercialization of students as natural learning materials and pet insects.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101156061B1 (en) | 2009-05-22 | 2012-06-27 | 대한민국 | Method of rearing cherry longicorn beetle, Aromia bungii, composition of feed for larva and composition of feed for imago |
| WO2013176441A1 (en) * | 2012-05-23 | 2013-11-28 | Yi Dae Am | Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same |
| CN105918256A (en) * | 2016-03-23 | 2016-09-07 | 中国农业科学院蔬菜花卉研究所 | Simple method for artificial feeding of chinese chive maggots |
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| KR102132168B1 (en) * | 2017-10-11 | 2020-07-09 | 우태명 | Method for producing larva of cicadas to shorten the hatching period and method for breeding cicadas using the same |
| CN109874753B (en) * | 2019-04-12 | 2023-06-13 | 广州蛰龙生态科技股份有限公司 | Propagation device and propagation method for insecticidal microorganisms |
| CN110402898B (en) * | 2019-08-16 | 2021-10-19 | 云南省热带作物科学研究所 | Method for obtaining standard test insects of rhaponticum uniflorum |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101156061B1 (en) | 2009-05-22 | 2012-06-27 | 대한민국 | Method of rearing cherry longicorn beetle, Aromia bungii, composition of feed for larva and composition of feed for imago |
| WO2013176441A1 (en) * | 2012-05-23 | 2013-11-28 | Yi Dae Am | Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same |
| CN105918256A (en) * | 2016-03-23 | 2016-09-07 | 中国农业科学院蔬菜花卉研究所 | Simple method for artificial feeding of chinese chive maggots |
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