WO2013176441A1 - Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same - Google Patents

Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same Download PDF

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Publication number
WO2013176441A1
WO2013176441A1 PCT/KR2013/004362 KR2013004362W WO2013176441A1 WO 2013176441 A1 WO2013176441 A1 WO 2013176441A1 KR 2013004362 W KR2013004362 W KR 2013004362W WO 2013176441 A1 WO2013176441 A1 WO 2013176441A1
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larvae
vitamin
longevity
weight
parts
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PCT/KR2013/004362
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French (fr)
Korean (ko)
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이대암
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Yi Dae Am
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Priority to JP2015513889A priority Critical patent/JP2015520610A/en
Priority to RU2015101919/13A priority patent/RU2605749C2/en
Priority to CN201380027010.8A priority patent/CN104334031B/en
Priority to CA2874428A priority patent/CA2874428C/en
Publication of WO2013176441A1 publication Critical patent/WO2013176441A1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/32—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from hydrolysates of wood or straw
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders

Definitions

  • the present invention relates to an artificial feed, a method of manufacturing artificial feed, and a method of breeding a longevity sky cow using the same for the multiplication of longevity sky cattle.
  • insects inhabiting the earth has not been accurately identified, but it is estimated that there are about 5 to 10 million species. Among them, about 15,000 species are directly or indirectly related to humans. Insects are attracting attention as the largest undeveloped biomass in the 21st century with such abundant species diversity. Attention is focused on the application.
  • Callipogon relictus is an insect belonging to the coleopteran celestial family, inhabiting the forests where old and large trees, such as acer, scorpion and ash, grow. .
  • the body length is 12cm for males and 7-8cm for females.
  • the body is black or blackish brown, and the gloss is strong.
  • the longevity sky cow is a rare insect with a high biological classification and distribution, and is protected as a natural monument.
  • the exhibitions, exhibition halls, ecological centers, and experiential learning centers using insects are continuously increasing.
  • the demand for pets is gradually increasing.
  • the present inventors have made intensive research to overcome the problems associated with mass breeding of longevity sky cows, and have developed artificial feeds optimized for breeding longevity sky cows, and in the case of breeding longevity sky cows by feeding them, mass production of longevity sky cows After confirming that they can, the present invention was completed.
  • the main object of the present invention is to provide an artificial feed and a method of manufacturing the same that can be grown indoors of longevity sky cattle.
  • Another object of the present invention is to significantly shorten the larvae, which take 5 to 6 years in the natural state to 3.8 years, and to increase the hatching rate of the larvae and the adult allegory, by setting the proper breeding environment of the longevity sky cow, It is to provide a breeding method that can multiply.
  • the present invention comprises a powdered oak sawdust and powder mixture of immersed oak irradiated with microwave at 50 ° C. to 60 ° C. and dried and pulverized to have a water content of 1% to 5%.
  • the present invention is irradiated with microwave at 50 °C to 60 °C immersed oak, dried to hydrate 1% to 5% and pulverized to produce oak sawdust, and then mixed with the powder mixture to the oak sawdust It provides a method for producing artificial feed for longevity sky cow, characterized in that.
  • the oak of the present invention is crushed through the process of irradiating the microwave after immersion, moisture absorbed by the oak tissue during the immersion process is activated in the process of irradiating microwave to cause fine damage to the oak tissue structure tissue structure This process changes the oak tree so that it can be consumed by longevity sky cows.
  • the microwave irradiation process also plays an additional role in removing eggs or larvae of ants or other predatory insects that may be competitors of bacteria, fungi, or potentially long-lived larvae in idle oaks. do.
  • the microwave may be used at any frequency suitable for the activation of moisture, but preferably microwaves between 300 MHz and 3 GHz, which are generally well absorbed by water and resistant to sterilization, and more preferably, resonant frequency of water. It is recommended that you use a microwave in the same 2540MHz band.
  • any oak can be used, but it is preferable to use an oak that is moderately decayed, and most preferably, oak oak that is not completely decayed in consideration of the feeding characteristics of the longevity sky cow is most preferred. At this time, it is good to remove the outer bark of oak completely.
  • 'Fallwood' refers to a rotten tree that is corrupted by microorganisms such as mushrooms.
  • oak having a length of 10 cm x 10-12 cm in diameter.
  • Drying of the oak is preferably 1% to 5% moisture content, when the moisture content is less than 1% has the disadvantage of increasing the drying time and cost, if more than 5% there is a risk that the oak will rot.
  • the dried and powdered oak sawdust is fed to the cellulose source of longevity larvae.
  • the crushed size of the oak sawdust is not particularly limited, but is preferably a size of 50 to 60 mesh for feeding convenience of longevity sky cattle.
  • the oak sawdust is preferably 95 to 97 parts by weight compared to 100 parts by weight of the artificial feed considering the nutritional balance and feeding characteristics of the longevity sky cow.
  • the powder mixture contains a mineral component necessary for longevity sky cow growth
  • the mineral component is calcium, magnesium, phosphorus, potassium, sodium, iron, manganese, zinc, and copper It is preferable to contain the minerals necessary for the raising of the sky cow.
  • the oak may be used by immersing in a vitamin mixed solution, wherein the vitamins used are ascorbic acid, traumatic acid, folic acid, pantothenic acid, biotin, vitamin B1, vitamin B2, vitamin B6, and inositol.
  • the vitamins that penetrate the oak microstructure, such as moisture are dispersed and absorbed evenly by the activating action of moisture during the irradiation of microwaves. Even if powdered, the vitamin components are evenly distributed in oak powder, thereby improving nutrition.
  • ascorbic acid added as the vitamin component may serve as a preservative to prevent the artificial feed from being rot due to low acidity.
  • the vitamin mixed solution is 0.05 to 0.1 parts by weight of ascorbic acid, 0.05 to 0.1 parts by weight, folic acid 0.0005 to 0.0015 parts by weight, pantothenic acid 0.003 to 0.007 parts by weight, biotin 0.0003 to 0.0007 parts by weight, based on 300 parts by weight of water, Vitamin mixed solution diluted by adding 0.0003 to 0.0007 parts by weight of vitamin B1, 0.0005 to 0.0009 parts by weight of vitamin B2, 0.0006 to 0.001 parts by weight of vitamin B6, and 0.1 to 0.3 parts by weight of inositol may be used.
  • long-lived sky cow artificial feed prepared by mixing microwave powder with dry oak can improve the early development of larvae, shorten larvae and days from hatching larvae to homeless larvae, and increase resistance to pathogens. It has the effect of improving the survival rate of the larva.
  • the present invention comprises the steps of preparing a seed larvae through homeless larvae by feeding and breeding artificial feed for breeding the place sky cattle at 20 °C, 60% humidity conditions of longevity sky cattle; And it provides a method for artificial proliferation of longevity sky cattle comprising the step of soluble in the pupal larvae stored.
  • the longevity sky cow artificial propagation method of the present invention first prepares hatching larvae of longevity sky cows, and supplies the artificial feed of the present invention to prepare a seed larvae after homeless larvae.
  • the hatching larvae of the longevity sky cow are not particularly limited, but may be hatched in a medium using the artificial feed of the present invention by collecting eggs of longevity sky cows from oak trees.
  • the period of preparing the larval larvae from the hatched larvae through the homeless larvae is about 1350 days.
  • the breeding temperature of the larvae is preferably maintained at 18 ° C. to 22 ° C. suitable for growth, and maintains a constant temperature of 20 ° C. Most preferably.
  • the hatching larvae are preferably one by one when the first larvae to breed in glass bottles to promote the feeding activity of longevity sky cattle.
  • the glass bottle is preferably cleaned, disinfected and exchanged for a certain period of time so as to keep the larvae free of disease.
  • Specimated larvae prepared in this way are released through the process of pupation and become pupa.
  • the solubilizing step is preferably performed at a temperature of 20 ° C. and a humidity of 60%.
  • breeding under the above conditions the breeding period of the long-term larvae can be significantly shortened by breaking the dormant period of the larvae.
  • the humidity of the preparation and the solubilizing step of the seed larvae is preferably maintained at 60%, when the larvae are raised at a humidity of more than 60% or less than 60%, so the larvae It is most desirable to breed at 60% humidity.
  • the humidity control step of preparing the larval larvae is carried out by directly spraying on the artificial feed, the humidity control step of the solubilization is sprayed directly on the body of the pupa water to remove the cause of the stress caused by floral foam It is preferable to carry out by supplying.
  • Antifungal agent when supplying moisture for the humidity control, a small amount of antifungal agent (Antifungal agent) can be mixed and administered in order to prevent the infection of harmful fungi in longevity sky cow, wherein the mixed antifungal agent butoconazole nitrate, clotri Mazole, ketoconazole nitrate, myconazole, antifungal polyene, nystatin, amphotericin B, fimaricin, oxyconazole nitrate, terconazole nitrate, thioconazole, flutrimazole, intraconazole, allyl Amines, terbenafine, butenapin, amorolpin, naphthypine, gluconazole, azole, echonasol, voriconazole, fluconazole, posaconazole, sulfonazole, dict bis-benzimidazole, glucan synthesis inhibitor, eki Nacandine, anidulafungin, ca
  • the solubilizing step may be performed in a photoperiod environment of 0L (description), 24D (memorization).
  • the survival rate of the larvae is significantly higher than the survival rate of the natural state, thereby increasing the solubilization rate and allegorization rate, shortening the larvae and days from hatching larvae to homeless larvae You can do it.
  • the long-lived sky cow which is conventionally impossible to breed artificially, can be grown throughout the year, but also the long-lived sky cow can be grown in large quantities throughout the year by increasing the survival rate and allegory rate of the larvae.
  • the longevity sky cow artificial feed according to the present invention includes oak powder irradiated with microwaves to improve the initial development of the larvae, shortening the larva diameter and days from hatching larvae to homeless larvae, and resistance to pathogens. Raising the effect of improving the survival rate of the larvae.
  • the breeding season of longevity sky cows can be breached, they can be grown throughout the year, and the longevity sky cows can be grown in large quantities throughout the year by increasing the survival rate and allegory rate of the larvae.
  • FIG. 1 is a flowchart illustrating a breeding method of a longevity sky cow according to the present invention.
  • the aqueous vitamin solution is used by mixing 85.8 mg of ascorbic acid, 75.3 mg of folic acid, folic acid 0.95 mg, pantothenic acid 4.6 mg, biotin 0.61 mg, vitamin B1 0.4 mg, vitamin B2 0.67 mg, vitamin B6 0.84 mg, and inositol 186 mg in 300 ml of water. It was.
  • 444 g of powder mixture was prepared by mixing 290 g of calcium, 70 g of magnesium, 150 g of phosphorus, 610 g of potassium, 20 g of sodium, 0.3 g of iron, 1.6 g of manganese, 2.34 g of zinc, and 0.2 g of copper.
  • Oak powder was prepared using oaks soaked with water instead of an aqueous vitamin solution, and vitamins were simply added to oak powders of the same type of vitamins used in Preparation Example 1 above. Except for the two points above, the artificial feed for longevity sky cattle was prepared using the same method as Preparation Example 1.
  • Hatching larvae were selected and used in the test to examine the developmental characteristics of longevity sky cows after vitamin addition and microwave irradiation treatment.
  • the hatching larvae initially secured 6 adults (3 females and 3 males), and then mated and spawned them to receive eggs on oak sub-trees, and then harvested long-lived larvae (82). Was carried out.
  • the larvae survival rate was measured at 200 days of the long-lived sky larvae by feeding the artificial feed prepared by Preparation Example 1, Preparation Example 2, and Comparative Example to the hatching larva selected as described above. Table 1 shows.
  • the artificial feed of Preparation Example 2 simply added vitamins in the experimental group 1-1 of the artificial feed of Preparation Example 1 using the aqueous vitamin solution It can be seen that the developmental characteristics are improved as compared with the experimental group 1-2, which is rapidly fed.
  • Experimental Example 1 was carried out using the artificial feed of Preparation Example 1 showing the best feed efficiency in order to find the optimal environmental conditions for the breeding of longevity sky larvae.
  • the breeding temperature is When the temperature was lowered to 15 °C, the solubility rate (40%) decreased, and when the raising temperature increased to 22 °C, the solubility rate (80%) decreased slightly, but when the temperature was further increased to 25 °C, the solubility rate rapidly increased to 20%. It tended to decrease.
  • Exclusive period means the number of days from the larva to yonghua
  • the lease period was the shortest at 13.4 days under the conditions of 20 ° C and 60% humidity, and the lease period increased with increasing temperature and humidity conditions. Based on these results, it was found that the larvae responded sensitively to changes in the breeding conditions.
  • the breeding conditions optimized for the larvae of larvae that could break the dormant of long-lived sky cows were 20 ° C and 60% humidity. I could see that.
  • Photoperiod condition suitable for breeding longevity Skylarva larvae was investigated under conditions (20 ° C., 60%) showing the highest solubility rate and allegory rate.
  • mice 4-1 to 4-3 which are long-term conditions in which light was administered for 12 hours or more per day, all larvae died, and mortality rate of larvae was reduced even under a single condition in which light was administered for 6 hours per day. Very high tendency was observed. All experimental larvae were lysed in experimental group 4-5, which had been reared for 24 hours a day, and all larvae were allegorized.

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Abstract

The present invention relates to artificial feed for the mass culture of long-horned beetles, to a method for manufacturing the artificial feed, and to a method for breeding long-horned beetles using same. The artificial feed for long-horned beetles according to the present invention contains oak powder irradiated with microwaves, and thus improves the initial development of larvae, decreases the larval age gap between hatched larvae and full-grown larvae, and improves the larval survival rate by increasing resistance to pathogenic bacteria and the like. In other words, according to the present invention, long-horned beetles which normally cannot be artificially bred can be bred year-round, and long-horned beetles can be bred in large quantities by increasing the larval survival rate and emergence rate.

Description

장수하늘소의 대량증식을 위한 인공사료 및 이의 제조방법, 이를 이용한 사육방법Artificial feed for mass breeding of longevity sky cows, manufacturing method thereof, and breeding method using the same
본 발명은 장수하늘소의 대량증식을 위한 인공사료, 인공사료의 제조방법, 및 이를 이용한 장수하늘소의 사육방법에 관한 것이다.The present invention relates to an artificial feed, a method of manufacturing artificial feed, and a method of breeding a longevity sky cow using the same for the multiplication of longevity sky cattle.
현재 지구상에 서식하고 있는 곤충의 종수는 정확히 파악된 바가 없으나, 약 500∼1,000만종 정도 서식하고 있는 것으로 추산되고 있다. 이중 인간과 직간접적으로 관련을 맺고 있는 종은 약 15,000여종으로 보고되고 있으며, 곤충은 이와 같은 풍부한 종 다양성과 더불어 21세기 최대 미개발 생물자원으로 주목받고 있으며, 농업분야에서 농가 소득 증대원으로 곤충의 활용에 관심이 집중되고 있다.The species of insects inhabiting the earth has not been accurately identified, but it is estimated that there are about 5 to 10 million species. Among them, about 15,000 species are directly or indirectly related to humans. Insects are attracting attention as the largest undeveloped biomass in the 21st century with such abundant species diversity. Attention is focused on the application.
현재 국내 곤충산업은 천적용 곤충분야, 화분매개용 곤충분야, 학습애완용 곤충분야, 식약용 곤충분야, 축제용 곤충 곤충분야 등으로 나눠볼 수 있는데, 최근 농림수산식품부에서는 ‘비전 2020’ 발표를 통해 2020년 곤충 산업을 7,000억원 이상의 시장규모로 육성한다는 계획을 수립하고 있고, 곤충산업은 정부 정책방향과 부합되어, 정부의 사업 지원에 따라 향후 성장성이 높을 것으로 전망된다.Currently, the domestic insect industry can be divided into natural insects, pollen-mediated insects, learning and pet insects, food and insect insects, and festival insect insects. Recently, the Ministry for Food, Agriculture, Forestry and Fisheries announced 'Vision 2020'. In 2020, the company plans to foster the insect industry to more than 700 billion won, and the insect industry is expected to grow in the future in line with government policy directions.
한편, 유려한 외관과 희귀성으로 인해 가장 인기 있는 곤충 중에 하나인 장수하늘소(Callipogon relictus)는 딱정벌레목 하늘소과에 속하는 곤충으로 서어나무, 신갈나무, 물푸레나무 등 오래되고 커다란 나무들이 자라는 숲에서 서식한다. 몸길이는 수컷 12㎝, 암컷 7∼8㎝ 정도이고, 몸은 검정색 또는 흑갈색을 띠며, 광택이 강하고 등에는 황갈색의 잔털이 빽빽하게 나 있다.On the other hand, one of the most popular insects due to its elegant appearance and rarity, Callipogon relictus is an insect belonging to the coleopteran celestial family, inhabiting the forests where old and large trees, such as acer, scorpion and ash, grow. . The body length is 12cm for males and 7-8cm for females. The body is black or blackish brown, and the gloss is strong.
장수하늘소는 생물 분류 및 분포학적 가치가 큰 진귀한 곤충으로 천연기념물로 지정되어 보호되고 있고, 곤충을 이용한 전시장, 박람회장, 생태원, 체험학습장 등이 지속적으로 늘어나는 등 곤충 산업이 성장하는 추세에 맞추어 학습이나 애완용으로 그 수요가 점진적으로 증가하고 있다. The longevity sky cow is a rare insect with a high biological classification and distribution, and is protected as a natural monument.In order to keep up with the growing trend of the insect industry, the exhibitions, exhibition halls, ecological centers, and experiential learning centers using insects are continuously increasing. The demand for pets is gradually increasing.
장수하늘소와 같은 희귀 곤충을 사육하기 위해서는 자연계에 존재하는 곤충의 먹이만으로 부족하기 때문에 이를 대량으로 사육할 수 있는 사료의 개발과 관련된 기술 개발이 필수적이며, 대상 곤충에 최적화된 사육 방법을 확립하는 것 역시 중요한 문제로 대두되고 있다.In order to raise rare insects such as longevity sky cows, it is necessary to develop the technology related to the development of feed that can breed large quantities because it is not enough to feed insects existing in nature, and to establish a breeding method optimized for the target insect. It is also an important issue.
그러나, 국내에서 곤충자원화 연구를 수행하기 위한 독립된 국가기관 또는 출연연구기관은 전무한 상태이고, 일부 대학, 국공립 연구소, 민간연구소 등에서 곤충사업화를 위한 연구를 수행하고 있으나, 유용곤충자원 개발은 벌, 굼벵이, 풍뎅이, 나비, 수선곤충류, 천적곤충류 등을 중심으로 일부 성과가 나타나고 있을 뿐, 장수하늘소의 실내 생리연구 및 생태학적 연구는 미비하여 장수하늘소의 대량증식이 어려운 실정이다. However, there are no independent national or research institutes for conducting insect resource research in Korea, and some universities, public and private research institutes are conducting research on insect commercialization, but useful insect resources are developed by bees and slugs. Some results have been shown mainly on scarabs, scarabs, butterflies, narcissus insects, and nemesis insects, but the indoor physiology and ecological studies of longevity sky cows are inadequate and mass breeding of longevity sky cows is difficult.
이에, 본 발명자들은 장수하늘소의 대량증식과 관련된 문제점들을 극복하기 위하여 예의 연구노력한 결과, 장수하늘소의 사육에 최적화된 인공사료를 개발하고, 이를 급여하여 장수하늘소를 사육하는 경우, 장수하늘소를 대량생산 할 수 있음을 확인하고, 본　발명을 완성하게 되었다.Therefore, the present inventors have made intensive research to overcome the problems associated with mass breeding of longevity sky cows, and have developed artificial feeds optimized for breeding longevity sky cows, and in the case of breeding longevity sky cows by feeding them, mass production of longevity sky cows After confirming that they can, the present invention was completed.
따라서, 본 발명의 주된 목적은 장수하늘소의 실내 대량증식이 가능한 인공사료와 이의 제조방법을 제공하는 데 있다.Therefore, the main object of the present invention is to provide an artificial feed and a method of manufacturing the same that can be grown indoors of longevity sky cattle.
본 발명의 다른 목적은 장수하늘소의 적정 사육환경 설정을 통하여 자연 상태에서 5 내지 6년 걸리는 유충기를 3.8년으로 대폭 단축시킬 뿐 아니라, 유충의 부화율과 성충의 우화율을 높여, 장수하늘소를 연중 대량 증식시킬 수 있는 사육방법을 제공하는데 있다.Another object of the present invention is to significantly shorten the larvae, which take 5 to 6 years in the natural state to 3.8 years, and to increase the hatching rate of the larvae and the adult allegory, by setting the proper breeding environment of the longevity sky cow, It is to provide a breeding method that can multiply.
상기의 목적을 달성하기 위해 본 발명은 수침한 참나무를 50℃ 내지 60℃에서 마이크로 웨이브를 조사하고 수분함량이 1% 내지 5%가 되도록 건조한 후 분쇄하여 분말화된 참나무 톱밥과 분말혼합물을 포함하는 장수하늘소 증식용 인공사료를 제공한다.In order to achieve the above object, the present invention comprises a powdered oak sawdust and powder mixture of immersed oak irradiated with microwave at 50 ° C. to 60 ° C. and dried and pulverized to have a water content of 1% to 5%. Provide artificial feed for longevity sky cow breeding.
또한, 본 발명은 수침한 참나무에 50℃ 내지 60℃에서 마이크로 웨이브를 조사하고, 수분함량이 1% 내지 5%가 되도록 건조한 후 분쇄하여 참나무 톱밥을 제조한 후, 상기 참나무 톱밥에 분말 혼합물을 혼합하는 것을 특징으로 하는 장수하늘소 증식용 인공사료의 제조방법을 제공한다.In addition, the present invention is irradiated with microwave at 50 ℃ to 60 ℃ immersed oak, dried to hydrate 1% to 5% and pulverized to produce oak sawdust, and then mixed with the powder mixture to the oak sawdust It provides a method for producing artificial feed for longevity sky cow, characterized in that.
본 발명의 참나무는 수침한 후 마이크로 웨이브를 조사하는 과정을 거쳐 분쇄되는데, 수침 과정에서 참나무 조직에 충분하게 흡수된 수분은 마이크로 웨이브를 조사하는 과정에서 활성화 되어 참나무 조직 구조에 미세 손상을 일으켜 조직구조를 변화시키게 되고, 이러한 과정을 통해 장수하늘소가 섭취하게 좋게 참나무를 가공하는 역할을 수행한다. 또한, 상기 마이크로 웨이브 조사 처리 과정을 통해 부휴 참나무에 존재하는 세균, 진균, 또는 잠재적으로 장수하늘소 유충의 경쟁자가 될 수 있는 개미나 다른 포식성 곤충들의 알이나 유충을 제거할 수 있는 부가적인 역할도 수행한다. The oak of the present invention is crushed through the process of irradiating the microwave after immersion, moisture absorbed by the oak tissue during the immersion process is activated in the process of irradiating microwave to cause fine damage to the oak tissue structure tissue structure This process changes the oak tree so that it can be consumed by longevity sky cows. The microwave irradiation process also plays an additional role in removing eggs or larvae of ants or other predatory insects that may be competitors of bacteria, fungi, or potentially long-lived larvae in idle oaks. do.
상기 마이크로 웨이브는 수분의 활성화에 적합한 어떠한 주파수의 마이크로 웨이브도 사용 가능하나, 바람직하게는 통상적으로 물에 잘 흡수되고 살균력이 강한 300MHz 내지 3GHz 사이의 극초단파를 사용할 수 있고, 더욱 바람직하게는 물의 공진주파수와 동일한 2540MHz 대역의 마이크로 웨이브를 사용하는 것이 좋다.The microwave may be used at any frequency suitable for the activation of moisture, but preferably microwaves between 300 MHz and 3 GHz, which are generally well absorbed by water and resistant to sterilization, and more preferably, resonant frequency of water. It is recommended that you use a microwave in the same 2540MHz band.
본 발명에서 사용되는 참나무는 어떠한 참나무도 사용 가능하나, 적당히 부패한 참나무를 사용하는 것이 바람직하며, 장수하늘소의 섭식 특성을 고려하여 심부가 완전히 부패하지 않은 참나무 부휴목을 사용하는 것이 가장 바람직하다. 이때, 참나무의 겉껍질을 완전히 제거하고 사용하는 것이 좋다. '부휴목'은 버섯 등의 미생물에 의해 부패하여 썩은 나무를 지칭한다. As the oak used in the present invention, any oak can be used, but it is preferable to use an oak that is moderately decayed, and most preferably, oak oak that is not completely decayed in consideration of the feeding characteristics of the longevity sky cow is most preferred. At this time, it is good to remove the outer bark of oak completely. 'Fallwood' refers to a rotten tree that is corrupted by microorganisms such as mushrooms.
마이크로 웨이브를 조사하는 과정에서 편의성과 수분 활성화를 통한 참나무 조직의 미세 손상 효율, 그리고 장수하늘소의 섭식 특성을 고려할 때, 상기 참나무는 길이 10cm x 직경 10~12cm의 크기의 참나무를 사용하는 것이 바람직하다.Considering the convenience, fine damage efficiency of oak tissues through the activation of moisture, and feeding characteristics of longevity cattle in the process of microwave irradiation, it is preferable to use oak having a length of 10 cm x 10-12 cm in diameter. .
마이크로 웨이브를 조사하는 과정에서 수침된 참나무는 자연스럽게 건조되는데, 이렇게 수분이 제거된 참나무 또는 참나무 분말은 쉽게 부패하지 않으므로 보관성이 개선되는 장점이 있다.Oak soaked in the process of irradiating the microwave is naturally dried, the oak or oak powder is removed from this moisture is not easily decay has the advantage of improved storage.
참나무의 건조는 수분함량이 1% 내지 5%가 바람직한데, 수분함량이 1% 미만이면 건조 시간 및 비용이 증가하는 단점이 있고, 5%를 초과하는 경우 참나무가 부패할 우려가 있다. Drying of the oak is preferably 1% to 5% moisture content, when the moisture content is less than 1% has the disadvantage of increasing the drying time and cost, if more than 5% there is a risk that the oak will rot.
이렇게 건조되어 분말화된 참나무 톱밥은 장수하늘소 유충의 셀룰로오스 원으로 공급된다. 상기 참나무 톱밥의 분쇄 크기는 특별히 제한되지는 않으나 장수하늘소의 섭식 편의성을 위하여 50 내지 60 메쉬의 크기인 것이 바람직하다. The dried and powdered oak sawdust is fed to the cellulose source of longevity larvae. The crushed size of the oak sawdust is not particularly limited, but is preferably a size of 50 to 60 mesh for feeding convenience of longevity sky cattle.
본 발명의 장수하늘소 증식용 인공사료에서, 상기 참나무 톱밥은 장수하늘소의 영양성분 균형과 섭식특성을 고려할 때 상기 인공사료 100 중량부 대비 95 내지 97 중량부인 것이 바람직하다. In the artificial feed for longevity sky cow growth of the present invention, the oak sawdust is preferably 95 to 97 parts by weight compared to 100 parts by weight of the artificial feed considering the nutritional balance and feeding characteristics of the longevity sky cow.
본 발명의 장수하늘소 증식용 인공사료에서, 상기 분말혼합물은 장수하늘소 증식에 필요한 미네랄 성분을 포함하되, 상기 미네랄 성분은 칼슘, 마그네슘, 인, 칼륨, 나트륨, 철, 망간, 아연, 및 동 등 장수하늘소의 사육에 필요한 미네랄이 포함되는 것이 바람직하다.In the artificial feed for longevity sky cow growth of the present invention, the powder mixture contains a mineral component necessary for longevity sky cow growth, the mineral component is calcium, magnesium, phosphorus, potassium, sodium, iron, manganese, zinc, and copper It is preferable to contain the minerals necessary for the raising of the sky cow.
또한, 상기 참나무는 비타민 혼합 용액에 수침하여 사용될 수 있는데, 이때 사용되는 비타민은 아스코르빈산, 유린, 엽산, 판토텐산(pantothenic acid), 비오틴, 비타민 B1, 비타민 B2, 비타민 B6, 이노시톨이 있다. 이렇게 비타민 혼합 용액을 사용하여 수침하는 경우 참나무 미세조직에 수분과 같이 침투된 비타민들이 마이크로 웨이브를 조사 과정에서 수분의 활성화 작용을 통해 비타민 성분이 참나무에 고르게 분산, 흡수되므로, 이 후 참나무가 분쇄되어 분말화 되어도 상기 비타민 성분들이 참나무 분말에 고르게 분포하게 되어 영양성이 개선되는 효과가 있다. 또한, 상기 비타민 성분으로 첨가되는 아스코르빈산은 낮은 산도로 인하여 제조된 인공사료가 부패하는 것을 방지하는 방부제 역할을 수행할 수 있다.In addition, the oak may be used by immersing in a vitamin mixed solution, wherein the vitamins used are ascorbic acid, traumatic acid, folic acid, pantothenic acid, biotin, vitamin B1, vitamin B2, vitamin B6, and inositol. In this case, when the vitamin mixture solution is immersed, the vitamins that penetrate the oak microstructure, such as moisture, are dispersed and absorbed evenly by the activating action of moisture during the irradiation of microwaves. Even if powdered, the vitamin components are evenly distributed in oak powder, thereby improving nutrition. In addition, ascorbic acid added as the vitamin component may serve as a preservative to prevent the artificial feed from being rot due to low acidity.
이때, 상기 비타민 혼합 용액은 물 300 중량부를 기준으로 아스코르빈산 0.05 내지 0.1 중량부, 유린 0.05 내지 0.1 중량부, 엽산 0.0005 내지 0.0015 중량부, 판토텐산 0.003 내지 0.007 중량부, 비오틴 0.0003 내지 0.0007 중량부, 비타민 B1 0.0003 내지 0.0007 중량부, 비타민 B2 0.0005 내지 0.0009 중량부, 비타민 B6 0.0006 내지 0.001 중량부, 및 이노시톨 0.1 내지 0.3 중량부를 첨가하여 희석한 비타민 혼합 용액을 사용할 수 있다. At this time, the vitamin mixed solution is 0.05 to 0.1 parts by weight of ascorbic acid, 0.05 to 0.1 parts by weight, folic acid 0.0005 to 0.0015 parts by weight, pantothenic acid 0.003 to 0.007 parts by weight, biotin 0.0003 to 0.0007 parts by weight, based on 300 parts by weight of water, Vitamin mixed solution diluted by adding 0.0003 to 0.0007 parts by weight of vitamin B1, 0.0005 to 0.0009 parts by weight of vitamin B2, 0.0006 to 0.001 parts by weight of vitamin B6, and 0.1 to 0.3 parts by weight of inositol may be used.
이와 같이 마이크로 웨이브를 조사하여 건조한 참나무에 분말 혼합물을 섞어 제조된 장수하늘소 인공사료는 유충의 초기 발육을 좋게 하여 부화유충에서 노숙유충까지의 유충경과 일수를 단축시킬 뿐 아니라, 병원균 등에 대한 저항성을 높여 유충의 생존율을 향상시키는 효과가 있다.In this way, long-lived sky cow artificial feed prepared by mixing microwave powder with dry oak can improve the early development of larvae, shorten larvae and days from hatching larvae to homeless larvae, and increase resistance to pathogens. It has the effect of improving the survival rate of the larva.
본 발명의 다른 양태에 따르면, 본 발명은 장수하늘소의 부화유충을 20℃, 습도 60% 조건에서 상기 장소하늘소 증식용 인공사료를 공급하고 사육하여 노숙유충기를 거친 종령유충을 준비하는 단계; 및 상기 저장된 종령유충을 번데기로 용화시키는 단계를 포함하는 장수하늘소의 인공증식방법을 제공한다.According to another aspect of the present invention, the present invention comprises the steps of preparing a seed larvae through homeless larvae by feeding and breeding artificial feed for breeding the place sky cattle at 20 ℃, 60% humidity conditions of longevity sky cattle; And it provides a method for artificial proliferation of longevity sky cattle comprising the step of soluble in the pupal larvae stored.
구체적으로 본 발명의 장수하늘소 인공증식방법은 먼저 장수하늘소의 부화유충을 준비하고, 본 발명의 인공사료를 공급하여 노숙유충기를 거친 종령유충을 준비한다. 상기 장수하늘소의 부화유충은 특별히 제한되지는 않으나, 참나무 부휴목에서 장수하늘소의 난을 채집하여 본 발명의 인공사료를 사용한 배지에서 부화시킬 수 있다. 상기 부화유충에서 노숙유충기를 거친 종령유충을 준비하는 기간은 대략 1350일 정도이며, 이때, 유충의 사육온도는 생장에 적합한 18℃ 내지 22℃로 유지시켜주는 것이 좋고, 20℃의 항온 조건을 유지하는 것이 가장 바람직하다.Specifically, the longevity sky cow artificial propagation method of the present invention first prepares hatching larvae of longevity sky cows, and supplies the artificial feed of the present invention to prepare a seed larvae after homeless larvae. The hatching larvae of the longevity sky cow are not particularly limited, but may be hatched in a medium using the artificial feed of the present invention by collecting eggs of longevity sky cows from oak trees. The period of preparing the larval larvae from the hatched larvae through the homeless larvae is about 1350 days. At this time, the breeding temperature of the larvae is preferably maintained at 18 ° C. to 22 ° C. suitable for growth, and maintains a constant temperature of 20 ° C. Most preferably.
그리고, 상기 부화유충은 장수하늘소의 먹이 활동의 촉진을 위해 1령 유충일 때 한 마리씩 분리하여 유리병에서 사육하는 것이 바람직하다. 상기 유리병은 일정기간을 두고 세척, 소독하여 교환함으로써 청결을 유지하여 유충이 질병에 걸리지 않도록 하는 것이 바람직하다.In addition, the hatching larvae are preferably one by one when the first larvae to breed in glass bottles to promote the feeding activity of longevity sky cattle. The glass bottle is preferably cleaned, disinfected and exchanged for a certain period of time so as to keep the larvae free of disease.
이렇게 준비된 종령유충은 용화과정을 통하여 탈피하여 번데기가 된다. 상기 용화시키는 단계는 온도 20℃, 습도 60% 조건에서 수행되는 것이 바람직한데, 상기 조건에서 사육하는 경우 종령유충의 휴면기를 타파하여 장수하늘소의 사육기간을 획기적으로 단축시킬 수 있다.Specimated larvae prepared in this way are released through the process of pupation and become pupa. The solubilizing step is preferably performed at a temperature of 20 ° C. and a humidity of 60%. When breeding under the above conditions, the breeding period of the long-term larvae can be significantly shortened by breaking the dormant period of the larvae.
상기 종령유충을 준비하는 단계와 상기 용화시키는 단계의 습도는 60%로 유지하는 것이 바람직한데, 60%를 초과하거나 60% 미만의 습도에서 사육하는 경우에는 유충의 용화율이 저하되므로, 상기 유충은 60%의 습도 조건에서 사육하는 것이 가장 바람직하다.The humidity of the preparation and the solubilizing step of the seed larvae is preferably maintained at 60%, when the larvae are raised at a humidity of more than 60% or less than 60%, so the larvae It is most desirable to breed at 60% humidity.
이때, 종령유충을 준비하는 단계의 습도조절은 상기 인공사료에 직접 살수하여 수행되고, 상기 용화시키는 단계의 습도조절은 번데기의 몸체에 직접 살수되어 스트레스를 유발하는 원인을 제거하기 위해 플로랄폼에 수분을 공급하여 수행되는 것이 바람직하다. At this time, the humidity control step of preparing the larval larvae is carried out by directly spraying on the artificial feed, the humidity control step of the solubilization is sprayed directly on the body of the pupa water to remove the cause of the stress caused by floral foam It is preferable to carry out by supplying.
또한, 상기 습도조절을 위해 수분을 공급할 때, 장수하늘소에 유해한 진균류의 감염을 막기 위해 항진균제(Antifungal agent)를 소량 혼합하여 투여할 수 있는데, 이때 혼합되는 항진균제로는 부토코나졸 나이트레이트, 클로트리마졸, 케토코나졸 나이트레이트, 미코나졸, 항진균성 폴리엔, 니스타틴, 암포테리신 B, 피마리신, 옥시코나졸 나이트레이트, 테르코나졸 나이트레이트, 티오코나졸, 플루트리마졸, 인트라코니졸, 알릴아민, 테르베나핀, 부테나핀, 아모롤핀, 나프티핀, 글루코나졸, 아졸, 에코나졸, 보리코니졸, 플루코나졸, 포사코나졸, 설코나졸, 딕션 비스-벤지미다졸, 글루칸 합성 억제제, 에키나칸딘, 아니둘라펀진, 카스포펀진, 미카펀진, 디아페닐설폰, 서클로피록솔라민, 할로프로진, 톨나탄, 언데실레네이트 등이 있다. In addition, when supplying moisture for the humidity control, a small amount of antifungal agent (Antifungal agent) can be mixed and administered in order to prevent the infection of harmful fungi in longevity sky cow, wherein the mixed antifungal agent butoconazole nitrate, clotri Mazole, ketoconazole nitrate, myconazole, antifungal polyene, nystatin, amphotericin B, fimaricin, oxyconazole nitrate, terconazole nitrate, thioconazole, flutrimazole, intraconazole, allyl Amines, terbenafine, butenapin, amorolpin, naphthypine, gluconazole, azole, echonasol, voriconazole, fluconazole, posaconazole, sulfonazole, dict bis-benzimidazole, glucan synthesis inhibitor, eki Nacandine, anidulafungin, caspofungin, micafungin, diaphenylsulfone, circleropyroxolamine, haloprozin, tolnantane, undecylenate, and the like.
상기 용화시키는 단계는 0L(명기), 24D(암기)의 광주기 환경에서 수행될 수 있다.The solubilizing step may be performed in a photoperiod environment of 0L (description), 24D (memorization).
이렇게 온도 20℃, 습도 60%, 0L(명기), 24D(암기)의 광주기 환경에서 본 발명에 따라 저장된 유충을 본 발명의 인공사료를 공급하면서 사육하게 되면, 실내에서 인공적으로 장수하늘소 유충을 용화 및 우화시킬 수 있다.When the larva stored in accordance with the present invention in the photoperiod environment of the temperature 20 ℃, humidity 60%, 0L (memory), 24D (memorization) while feeding the artificial feed of the present invention, artificially longevity larvae indoors Solubilize and allegorize.
상기한 본 발명에 따른 장수하늘소의 증식방법에 따르면, 유충의 생존율이 자연 상태의 생존율에 비해 월등히 높아지고, 이로 인해 용화율 및 우화율이 매우 높아지며, 부화유충에서 노숙유충까지의 유충경과 일수를 단축시킬 수 있게 된다. 즉, 본 발명에 따르면 종래 인공적으로 사육이 불가능한 장수하늘소를 일년 내내 증식시킬 수 있을 뿐 아니라, 유충의 생존율 및 우화율을 높임으로써 장수하늘소를 일년 내내 대량으로 증식시킬 수 있게 된다.According to the growth method of the longevity sky cow according to the present invention, the survival rate of the larvae is significantly higher than the survival rate of the natural state, thereby increasing the solubilization rate and allegorization rate, shortening the larvae and days from hatching larvae to homeless larvae You can do it. In other words, according to the present invention, not only the long-lived sky cow, which is conventionally impossible to breed artificially, can be grown throughout the year, but also the long-lived sky cow can be grown in large quantities throughout the year by increasing the survival rate and allegory rate of the larvae.
이상 설명한 바와 같이, 본 발명에 따른 장수하늘소 인공사료는 마이크로 웨이브를 조사한 참나무 분말을 포함함으로써 유충의 초기 발육을 좋게 하여 부화유충에서 노숙유충까지의 유충경과 일수를 단축시킬 뿐 아니라, 병원균 등에 대한 저항성을 높여 유충의 생존율을 향상시킬 수 있는 효과가 있다.As described above, the longevity sky cow artificial feed according to the present invention includes oak powder irradiated with microwaves to improve the initial development of the larvae, shortening the larva diameter and days from hatching larvae to homeless larvae, and resistance to pathogens. Raising the effect of improving the survival rate of the larvae.
또한, 본 발명에 따르면 장수하늘소의 휴면기를 타파하여 일년 내내 증식시킬 수 있을 뿐 아니라, 유충의 생존율 및 우화율을 높임으로써 장수하늘소를 일년 내내 대량으로 증식시킬 수 있게 된다.In addition, according to the present invention, not only can the breeding season of longevity sky cows be breached, they can be grown throughout the year, and the longevity sky cows can be grown in large quantities throughout the year by increasing the survival rate and allegory rate of the larvae.
도 1은 본 발명에 따른 장수하늘소의 사육 방법을 도시한 흐름도이다. 1 is a flowchart illustrating a breeding method of a longevity sky cow according to the present invention.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.Hereinafter, the present invention will be described in more detail with reference to Examples. Since these examples are only for illustrating the present invention, the scope of the present invention is not to be construed as being limited by these examples.
[제조예 1] 비타민 수용액 침지 참나무 분말을 이용한 인공사료의 제조Preparation Example 1 Preparation of Artificial Feed Using Vitamin Aqueous Soaking Oak Powder
심부가 완전히 부패하지 않은 길이 10cm x 직경 10~12cm급 참나무를 겉껍질을 완전히 제거하고, 비타민 수용액에 2시간 동안 담근 후, 60℃에서 수분 3%가 될 때까지 전자렌지를 이용하여 마이크로 웨이브를 조사하여 참나무를 가열 건조시킨 다음 파쇄기로 파쇄하여 참나무 분말을 제조하였다. 10cm x 10 ~ 12cm in diameter oak, whose core is not completely decayed, is completely removed from the bark, soaked in a vitamin solution for 2 hours, and then microwaved using a microwave until it reaches 3% moisture at 60 ° C. The oak was irradiated with heat by drying and then crushed with a shredder to prepare oak powder.
상기 비타민 수용액은 물 300ml에 아스코르빈산 85.8mg, 유린 75.3mg, 엽산0.95mg, 판토텐산 4.6mg, 비오틴 0.61mg, 비타민 B1 0.4mg, 비타민 B2 0.67mg, 비타민 B6 0.84mg, 이노시톨 186mg을 혼합하여 사용하였다.The aqueous vitamin solution is used by mixing 85.8 mg of ascorbic acid, 75.3 mg of folic acid, folic acid 0.95 mg, pantothenic acid 4.6 mg, biotin 0.61 mg, vitamin B1 0.4 mg, vitamin B2 0.67 mg, vitamin B6 0.84 mg, and inositol 186 mg in 300 ml of water. It was.
칼슘 290g, 마그네슘 70g, 인 150g, 칼륨 610g, 나트륨 20g, 철 0.3g, 망간 1.6g, 아연 2.34g, 및 동 0.2g 을 혼합하여 분말혼합물 1444.44g을 제조하였다.444 g of powder mixture was prepared by mixing 290 g of calcium, 70 g of magnesium, 150 g of phosphorus, 610 g of potassium, 20 g of sodium, 0.3 g of iron, 1.6 g of manganese, 2.34 g of zinc, and 0.2 g of copper.
상기 참나무 분말 35kg과 상기 분말혼합물 1444.44g을 혼합하여 장수하늘소 사육용 인공사료를 제조하였다.35 kg of oak powder and 1444.44 g of the powder mixture were mixed to prepare artificial feed for raising longevity sky cattle.
[제조예 2] 비타민을 수용액 형태가 아닌 단순 첨가방식으로 사용하여 인공사료의 제조[Preparation Example 2] Preparation of artificial feed by using vitamin as a simple addition method rather than in the form of an aqueous solution
비타민 수용액 대신 물을 사용하여 수침한 참나무를 이용하여 참나무 분말을 제조하였고, 비타민은 상기 제조예 1에서 사용한 비타민과 같은 종류의 비타민을 참나무 분말에 단순히 첨가하여 사용하였다. 이상의 2가지 점 외에는 상기 제조예 1과 동일한 방법을 사용하여 장수하늘소 사육용 인공사료를 제조하였다.Oak powder was prepared using oaks soaked with water instead of an aqueous vitamin solution, and vitamins were simply added to oak powders of the same type of vitamins used in Preparation Example 1 above. Except for the two points above, the artificial feed for longevity sky cattle was prepared using the same method as Preparation Example 1.
[비교예] 마이크로 웨이브 비사용 인공사료의 제조Comparative Example Preparation of Non-Microwave Artificial Feed
마이크로 웨이브 조사를 통한 참나무의 가열 건조 공정을 대신하여, 수증기를 이용하여 15분간 살균하고, 건조기를 사용하여 건조하였다. 이상의 가열 건조 공정을 제외하고, 상기 제조예 1과 동일한 방법을 사용하여 장수하늘소 사육용 인공사료를 제조하였다.Instead of heating and drying the oak tree through microwave irradiation, it was sterilized for 15 minutes using steam and dried using a dryer. Except for the above heat drying step, using the same method as in Preparation Example 1 was prepared artificial feed for longevity sky cattle.
[실험예 1] 비타민 첨가 및 마이크로 웨이브 조사 처리에 따른 장수하늘소의 발육특성Experimental Example 1 Development of Longevity Sky Cow by Addition of Vitamin and Microwave Irradiation
비타민 첨가 및 마이크로 웨이브 조사 처리에 따른 장수하늘소의 발육 특성을 살펴보기 위하여 부화유충을 선발하여 시험에 사용하였다.Hatching larvae were selected and used in the test to examine the developmental characteristics of longevity sky cows after vitamin addition and microwave irradiation treatment.
부화유충은 초기에 성충 6마리(암컷 3두, 수컷 3두)를 확보한 후, 이 성충들을 교미, 산란시켜 참나무 부휴목에 알을 받은 뒤 장수하늘소 유충(82두)을 채취하여 인공사육을 실시하였다.The hatching larvae initially secured 6 adults (3 females and 3 males), and then mated and spawned them to receive eggs on oak sub-trees, and then harvested long-lived larvae (82). Was carried out.
이렇게 선발된 부화유충에 상기 제조예 1, 제조예 2, 및 비교예에 의해 제조된 인공사료를 급이하여 장수하늘소 유충의 200일 경과 시점에서 유충 생존율을 측정하였고, 유충 경과 기간을 측정하여 하기 표 1에 나타내었다.The larvae survival rate was measured at 200 days of the long-lived sky larvae by feeding the artificial feed prepared by Preparation Example 1, Preparation Example 2, and Comparative Example to the hatching larva selected as described above. Table 1 shows.
표 1
실험군 사료 종류 유충 생존률(%) 유충 경과(일)
1-1 제조예 1의 인공사료 98 1347
1-2 제조예 2의 인공사료 95 1373
1-3 비교예의 인공사료 87 1665
Table 1
Experimental group Feed type Larval survival rate (%) Larva progress (days)
1-1 Artificial feed of Preparation Example 1 98 1347
1-2 Artificial feed of Preparation Example 2 95 1373
1-3 Artificial Feed of Comparative Example 87 1665
상기 표 1을 살펴보면, 마이크로 웨이브 조사 처리 과정을 거쳐 제조된 제조예 1 및 제조예 2의 인공사료를 급이한 실험군 1-1 및 1-2가 마이크로 웨이브를 처리하지 않은 비교예의 인공사료를 급이한 실험군 1-3에 비해 200일 경과 시점에서 유충 생존율이 각각 11% 및 8% 증가한 수치를 보였다. Referring to Table 1, Experimental Groups 1-1 and 1-2, which fed artificial feeds of Preparation Example 1 and Preparation Example 2, prepared through microwave irradiation, were fed artificial feeds of Comparative Example, which did not process microwaves. Larval survival rates increased 11% and 8%, respectively, after 200 days compared to experimental groups 1-3.
이러한 결과를 통해 마이크로 웨이브 조사 처리 과정을 통하여 미세 조직 손상된 참나무 분말을 사용한 인공사료들이 마이크로 웨이브 조사 처리를 하지 않은 참나무 분말을 사용한 인공사료에 비하여 사료 효율이 개선되어 유충 생존율이 개선되는 것을 알 수 있었다.These results show that the microfeeds improved the feed efficiency compared to the artificial feeds using the oak powder without the micro-irradiated oak powder. .
그리고, 참나무를 수침할 때 사용한 수용액의 종류에 따른 발육특성을 비교할 때, 비타민 수용액을 사용한 제조예 1의 인공사료를 급이한 실험군 1-1에서, 비타민을 단순 첨가한 제조예 2의 인공사료를 급이한 실험군 1-2에 비하여 발육특성이 개선됨을 알 수 있었다. And, when comparing the growth characteristics according to the type of the aqueous solution used to immerse the oak, the artificial feed of Preparation Example 2 simply added vitamins in the experimental group 1-1 of the artificial feed of Preparation Example 1 using the aqueous vitamin solution It can be seen that the developmental characteristics are improved as compared with the experimental group 1-2, which is rapidly fed.
이러한 결과는 참나무 미세조직에 수분과 같이 침투된 비타민들이 마이크로 웨이브를 조사 과정에서 수분의 활성화 작용을 통해 비타민 성분이 참나무에 고르게 분산, 흡수되고, 이 후 참나무가 분쇄되어 분말화 되어도 상기 비타민 성분들이 참나무 분말에 고르게 분포하게 되어 영양성이 개선되기 때문인 것으로 사료된다.These results indicate that vitamins, such as moisture, penetrated into oak microstructures are dispersed and absorbed evenly by oaks through the activation of water during the irradiation of microwaves. This is because the nutrition is improved because it is evenly distributed in oak powder.
또한, 급이한 사료 종류에 따른 유충 경과를 살펴보면, 비타민 수용액을 사용하고, 마이크로 웨이브 조사 처리를 거친 제조예 1의 인공사료를 급이한 실험군 1-1에서 가장 짧은 유충 경과 기간이 관찰되었고, 마이크로 웨이브 조사처리를 거치지 않은 비교예의 사료를 급이한 실험군 1-3에서 가장 긴 유충 경과 기간이 관찰되었다. In addition, when looking at the larvae progression according to the rapid feed type, the shortest larval elapsed period was observed in the experimental group 1-1 using the vitamin aqueous solution and fed the artificial feed of Preparation Example 1 subjected to the microwave irradiation treatment, The longest larval elapsed time was observed in experimental groups 1-3, which fed the feed of the comparative example without microwave irradiation treatment.
이러한 결과들을 종합적으로 분석한 결과, 참나무 부휴목의 마이크로 웨이브 조사 처리 유무 및 비타민 수용액 침지 여부는 장수하늘소 인공사료의 사료 특성 및 장수하늘소 유충의 발육에 영향을 미치는 중요한 인자임을 알 수 있었다. As a result of comprehensive analysis, it was found that the presence or absence of microwave irradiation treatment and oak solution of vitamin A of oak groves were important factors affecting the feed characteristics of long-haul cattle artificial feed and the development of long-haul cattle larvae.
[실험예 2] 장수하늘소 유충의 사육 적정 환경 조건 확립Experimental Example 2 Establishment of Optimum Environmental Conditions for Breeding Longevity Cattle Larvae
2-1 부화유충에서 용화까지의 적정 사육환경2-1 Optimal breeding environment from hatching larvae to yonghwa
실험예 1에서 가장 좋은 사료 효율을 보인 제조예 1의 인공사료를 이용하여 장수하늘소 유충의 사육 적정 환경 조건을 찾기 위한 실험을 수행하였다.Experimental Example 1 was carried out using the artificial feed of Preparation Example 1 showing the best feed efficiency in order to find the optimal environmental conditions for the breeding of longevity sky larvae.
사육 적정 환경 조건을 확립하기 위해 장수하늘소의 부화유충 5마리를 1 Set로 하여 제조예 1의 인공사료를 공급하여 하기 표 2의 온습도 조건에 따라 사육하여 유충의 용화율과 우화율 변화를 관찰하였다. In order to establish proper environmental conditions, five hatching larvae of longevity larvae were set as one set, and artificial feeds of Preparation Example 1 were fed to breed according to the temperature and humidity conditions of Table 2 to observe the larval solubility and allegory rate. .
표 2
실험군 온도 습도 용화율 우화율
2-1 15℃ 60% 40% 40%
2-2 18℃ 60% 100% 100%
2-3 20℃ 60% 100% 100%
2-4 22℃ 60% 80% 80%
2-5 25℃ 60% 20% 20%
2-6 20℃ 40% 40% 80%
2-7 20℃ 50% 80% 80%
2-8 20℃ 70% 80% 80%
2-9 20℃ 80% 40% 80%
TABLE 2
Experimental group Temperature Humidity Dissolution rate Allegory
2-1 15 ℃ 60% 40% 40%
2-2 18 ℃ 60% 100% 100%
2-3 20 ℃ 60% 100% 100%
2-4 22 ℃ 60% 80% 80%
2-5 25 ℃ 60% 20% 20%
2-6 20 ℃ 40% 40% 80%
2-7 20 ℃ 50% 80% 80%
2-8 20 ℃ 70% 80% 80%
2-9 20 ℃ 80% 40% 80%
상기 표 2에 나타난 바와 같이, 습도를 60%로 동일하게 하고, 온도에 변화를 준 실험군 2-1 내지 2-5를 살펴보면, 18℃와 20℃에서 사육된 유충들은 모두 용화되었고, 사육온도가 15℃로 낮아지면 용화율(40%)이 감소하였으며, 사육 온도가 22℃로 상승하면 용화율(80%)이 다소 감소하다가, 온도를 25℃로 더욱 상승시키면 용화율이 20%로 급격하게 감소하는 경향을 보였다.As shown in Table 2, when the humidity was the same as 60%, and the experimental group 2-1 to 2-5 with a change in temperature, the larvae raised at 18 ℃ and 20 ℃ were all dissolved, the breeding temperature is When the temperature was lowered to 15 ℃, the solubility rate (40%) decreased, and when the raising temperature increased to 22 ℃, the solubility rate (80%) decreased slightly, but when the temperature was further increased to 25 ℃, the solubility rate rapidly increased to 20%. It tended to decrease.
또한, 온도를 20℃로 동일하게 하고, 습도에 변화를 준 실험군 2-3, 및 2-6 내지 2-9를 살펴보면, 습도 60%를 기준으로 습도가 증감할수록 용화율이 지속적으로 감소하는 경향을 보였다.In addition, when the temperature is the same as 20 ℃, and the experimental group 2-3, and 2-6 to 2-9 with a change in humidity, the degree of solubility is continuously decreased as the humidity increases or decreases based on 60% humidity. Showed.
이러한 결과를 바탕으로, 장수하늘소 유충의 용화율 향상을 위해서는 18℃ 내지 22℃의 온도조건에서 50% 내지 70% 습도조건을 유지하면서 사육하는 것이 바람직한 것으로 나타났으며, 20℃의 온도조건에서 60% 습도조건을 유지하면서 사육하는 것이 가장 바람직한 것으로 나타났다. 또한, 상기 표 2의 결과에서, 일단 용화한 장수하늘소 유충은 거의 100% 우화하는 것으로 나타났다.Based on these results, in order to improve the solubilization rate of long-lived sky larvae, it was found that breeding is preferably maintained at 50 to 70% humidity conditions at a temperature of 18 ℃ to 22 ℃, 60 at a temperature of 20 ℃ Breeding while maintaining humidity conditions has been shown to be most desirable. In addition, in the results of Table 2, the long-lived sky larvae once dissolved, was found to be almost 100% allegory.
2-2 종령유충에서 용화까지의 적정사육환경2-2 Optimal Breeding Environment from Species Larvae to Longhua
장수하늘소의 사육에서 가장 중요한 단계인 종령유충에서 용화에 들어가는 단계에서 최적화된 사육 환경을 찾아보고자 하기의 실험을 수행하였다. The following experiment was conducted to find the optimal breeding environment in the stage of larvae in the larval larvae, the most important stage in the breeding of longevity sky cattle.
장수하늘소의 종령유충 5마리를 1 세트로 하여 제조예 1의 인공사료를 공급하여 하기 표 3의 온습도 조건에 따라 사육하여 유충의 용화율, 전용기간, 및 우화율 변화를 관찰하였다. Five larvae of longevity larvae were fed as a set of artificial feeds of Preparation Example 1, and were bred according to the temperature and humidity conditions of Table 3 to observe the larvae dissolution rate, lease duration, and allegory rate.
표 3
실험군 온도 습도 용화율 전용기간* 우화율
3-1 15℃ 60% 40% 20.9일 40%
3-2 18℃ 60% 100% 15.7일 100%
3-3 20℃ 60% 100% 13.4일 100%
3-4 22℃ 60% 80% 16.5일 80%
3-5 25℃ 60% 20% 18.4일 20%
3-6 20℃ 40% 40% 16.7일 80%
3-7 20℃ 50% 80% 14.3일 80%
3-8 20℃ 70% 80% 17.6일 80%
3-9 20℃ 80% 40% 15.7일 80%
TABLE 3
Experimental group Temperature Humidity Dissolution rate Expiry date * Allegory
3-1 15 ℃ 60% 40% 20.9 days 40%
3-2 18 ℃ 60% 100% 15.7 days 100%
3-3 20 ℃ 60% 100% 13.4 days 100%
3-4 22 ℃ 60% 80% 16.5 days 80%
3-5 25 ℃ 60% 20% 18.4 days 20%
3-6 20 ℃ 40% 40% 16.7 days 80%
3-7 20 ℃ 50% 80% 14.3 days 80%
3-8 20 ℃ 70% 80% 17.6 days 80%
3-9 20 ℃ 80% 40% 15.7 days 80%
*전용기간은 종령유충에서 용화까지의 일수를 의미함* Exclusive period means the number of days from the larva to yonghua
상기 표 3에 나타난 바와 같이, 사육 조건에 따른 종령유충의 용화율 및 우화율은 상기 실험예 2-1의 부화유충의 용화율 및 우화율 변화 결과와 동일하게 나타났으나, 전용기간에서는 차이가 있었다. As shown in Table 3, the solubilization rate and allegorization rate of the seedling larvae according to the breeding conditions were the same as the change rate of the hatching larvae and the allegory rate of the hatching larva of Experimental Example 2-1, but the difference was found in the dedicated period. there was.
즉, 종령유충이 용화되는 전용기간을 살펴보면, 온도 20℃, 습도 60%의 조건에서 전용기간이 13.4일로 가장 짧게 나타났고, 온도나 습도조건이 증감할수록 전용기간이 증가하는 결과를 보였다. 이러한 결과를 바탕으로 종령유충은 사육조건의 변화에 따라 민감하게 반응하는 것을 알 수 있었고, 장수하늘소의 휴면기를 타파할 수 있는 종령유충의 용화에 최적화된 사육조건은 20℃, 60% 습도 조건인 것을 알 수 있었다. In other words, when the larvae were soluble, the lease period was the shortest at 13.4 days under the conditions of 20 ° C and 60% humidity, and the lease period increased with increasing temperature and humidity conditions. Based on these results, it was found that the larvae responded sensitively to changes in the breeding conditions. The breeding conditions optimized for the larvae of larvae that could break the dormant of long-lived sky cows were 20 ° C and 60% humidity. I could see that.
[실험예 3] 장수하늘소 유충 사육에 적정한 광주기 조건 확립Experimental Example 3 Establishment of Photoperiod Condition Suitable for Longevity Cattle Larvae Breeding
상기 장수하늘소 유충이 가장 높은 용화율 및 우화율을 보이는 조건(20℃, 60%)에서 장수하늘소 유충 사육에 적정한 광주기 조건을 조사하였다. Photoperiod condition suitable for breeding longevity Skylarva larvae was investigated under conditions (20 ° C., 60%) showing the highest solubility rate and allegory rate.
사육 적정 광주기 조건을 확립하기 위해 장수하늘소의 부화유충 5마리를 1 Set로 하여 제조예 1의 인공사료를 공급하여 하기 표 4의 광주기 조건에 따라 사육하여 유충의 용화율과 우화율 변화를 관찰하였다. In order to establish proper photoperiod condition, five hatching larvae of longevity sky cow were set as 1 set, and artificial feed of Preparation Example 1 was supplied to breed according to photoperiod conditions of Table 4 to change the larval solubility and allegory rate. Observed.
표 4
실험군 광주기 조건 용화율(%) 우화율(%)
4-1 24L·0D - -
4-2 18L·6D - -
4-3 12L·12D - -
4-4 6L·18D 20% 20%
4-5 0L·24D 100% 100%
Table 4
Experimental group Photoperiod condition Dissolution Rate (%) Fable Rate (%)
4-1 24L, 0D - -
4-2 18L, 6D - -
4-3 12L, 12D - -
4-4 6L, 18D 20% 20%
4-5 0L, 24D 100% 100%
상기 표 4에 나타낸 바와 같이, 하루 12시간 이상 빛을 투여한 장일 조건인 실험군 4-1 내지 4-3에서는 모든 유충들이 폐사하였고, 하루 6시간의 빛을 투여한 단일 조건하에서도 유충의 폐사율이 매우 높은 경향이 관찰되었다. 하루 24시간동안 암기에서 사육한 실험군 4-5에서는 모든 유충이 용화되었고, 용화된 유충들은 모두 우화하였다. As shown in Table 4, in experimental groups 4-1 to 4-3, which are long-term conditions in which light was administered for 12 hours or more per day, all larvae died, and mortality rate of larvae was reduced even under a single condition in which light was administered for 6 hours per day. Very high tendency was observed. All experimental larvae were lysed in experimental group 4-5, which had been reared for 24 hours a day, and all larvae were allegorized.
결론적으로 0L·24D의 광주기 조건에서 장수하늘소를 용화시키는 것이 가장 바람직한 것을 알 수 있었다.In conclusion, it was found that it is most desirable to solubilize the longevity sky cow under the photoperiod condition of 0L · 24D.

Claims (12)

  1. 참나무 톱밥 및 분말혼합물을 포함하는 장수하늘소 증식용 인공사료의 제조방법으로서, As a manufacturing method of artificial feed for longevity sky cow growth comprising oak sawdust and powder mixture,
    수침한 참나무에 50℃ 내지 60℃에서 마이크로 웨이브를 조사하고, 수분함량이 1% 내지 5%가 되도록 건조한 후 분쇄하여 참나무 톱밥을 제조한 후,Irradiated microwaves at 50 ℃ to 60 ℃ to immersed oak, dried to hydrate 1% to 5% and then pulverized to produce oak sawdust,
    상기 참나무 톱밥에 분말 혼합물을 혼합하는 것을 특징으로 하는 장수하늘소 증식용 인공사료의 제조방법.The method of producing artificial feed for longevity sky cow, characterized in that the powder mixture is mixed with the oak sawdust.
  2. 제 1 항에 있어서, The method of claim 1,
    상기 참나무 톱밥은 상기 인공사료 100 중량부 대비 95 내지 97 중량부가 되도록 혼합하는 것을 특징으로 하는 장수하늘소 증식용 인공사료의 제조방법.The oak sawdust is a method for producing artificial feed for longevity sky cow, characterized in that the mixing so as to 95 to 97 parts by weight compared to 100 parts by weight of the artificial feed.
  3. 제 1 항에 있어서, The method of claim 1,
    상기 분말혼합물은 장수하늘소 증식에 필요한 미네랄 성분을 포함하되, 상기 미네랄 성분은 칼슘, 마그네슘, 인, 칼륨, 나트륨, 철, 망간, 아연, 및 동에서 선택된 하나 이상인 것을 특징으로 하는 장수하늘소 증식용 인공사료의 제조방법.The powder mixture includes a mineral component necessary for longevity sky cow growth, wherein the mineral ingredient is at least one selected from calcium, magnesium, phosphorus, potassium, sodium, iron, manganese, zinc, and copper artificial proliferation Method of making feed.
  4. 제 1 항에 있어서, The method of claim 1,
    상기 수침한 참나무는 비타민 혼합 용액에 수침한 것을 특징으로 하는 장수하늘소 증식용 인공사료의 제조방법.The impregnated oak is a method of producing artificial feed for longevity sky cattle, characterized in that the solution is immersed in a vitamin mixed solution.
  5. 제 4 항에 있어서, The method of claim 4, wherein
    상기 비타민 혼합 용액은 아스코르빈산, 유린, 엽산, 판토텐산, 비오틴, 비타민 B1, 비타민 B2, 비타민 B6, 및 이노시톨에서 선택된 하나 이상의 비타민을 포함한 것을 특징으로 하는 장수하늘소 증식용 인공사료의 제조방법.The vitamin mixed solution is ascorbic acid, euolin, folic acid, pantothenic acid, biotin, vitamin B1, vitamin B2, vitamin B6, and method for producing artificial feed for longevity sky cow, characterized in that it comprises at least one vitamin selected from inositol.
  6. 제 5 항에 있어서, The method of claim 5,
    상기 비타민 혼합 용액은 물 300 중량부를 기준으로 아스코르빈산 0.05 내지 0.1 중량부, 유린 0.05 내지 0.1 중량부, 엽산 0.0005 내지 0.0015 중량부, 판토텐산 0.003 내지 0.007 중량부, 비오틴 0.0003 내지 0.0007 중량부, 비타민 B1 0.0003 내지 0.0007 중량부, 비타민 B2 0.0005 내지 0.0009 중량부, 비타민 B6 0.0006 내지 0.001 중량부, 및 이노시톨 0.1 내지 0.3 중량부를 첨가하여 희석한 비타민 혼합 용액인 것을 특징으로 하는 장수하늘소 증식용 인공사료의 제조방법.The vitamin mixed solution is 0.05 to 0.1 parts by weight of ascorbic acid, 0.05 to 0.1 parts by weight, folic acid 0.0005 to 0.0015 parts by weight, pantothenic acid 0.003 to 0.007 parts by weight, biotin 0.0003 to 0.0007 parts by weight, vitamin B1 based on 300 parts by weight of water Method for producing artificial feed for longevity sky cow, characterized in that the vitamin mixed solution diluted by adding 0.0003 to 0.0007 parts by weight, vitamin B2 0.0005 to 0.0009 parts by weight, vitamin B6 0.0006 to 0.001 parts by weight, and 0.1 to 0.3 parts by weight of inositol. .
  7. 장수하늘소의 부화유충을 18℃ 내지 22℃, 습도 50 내지 70% 조건에서 제1항 내지 제6항 중 어느 한 항의 방법으로 제조된 장소하늘소 증식용 인공사료를 공급하고 사육하여 노숙유충기를 거친 종령유충을 준비하는 단계; 및 The breeding larvae of livestock larvae fed and bred the artificial larvae grown in the place of any one of claims 1 to 6 at 18 ° C. to 22 ° C. and humidity of 50 to 70%. Preparing the larvae; And
    상기 준비된 종령유충을 번데기로 용화시키는 단계;를 포함하는 장수하늘소의 인공증식방법.Long-lived sky cow artificial propagation method comprising the;
  8. 제 7 항에 있어서, The method of claim 7, wherein
    상기 부화유충은 1령 유충일 때 한 마리씩 분리하여 유리병에서 사육되는 것을 특징으로 하는 장수하늘소의 인공증식방법.When the hatching larvae are separated by one when the larvae 1 breeding method of the longevity sky cow, characterized in that the breeding in a glass bottle.
  9. 제 7 항에 있어서, The method of claim 7, wherein
    상기 용화시키는 단계는 온도 20℃, 습도 60% 조건에서 수행되는 것을 특징으로 하는 장수하늘소의 인공증식방법.The solubilizing step is a method of artificial growth of longevity sky cow, characterized in that carried out at a temperature of 20 ℃, 60% humidity conditions.
  10. 제 9 항에 있어서, The method of claim 9,
    상기 종령유충을 준비하는 단계의 습도조절은 상기 인공사료에 살수하여 수행되고, 상기 용화시키는 단계의 습도조절은 플로랄폼에 수분을 공급하여 수행되는 것을 특징으로 하는 장수하늘소의 인공증식방법.Humidity control of the step of preparing the seed larvae is carried out by watering the artificial feed, humidity control of the solubilizing step is a method of artificial breeding of longevity sky cow, characterized in that is performed by supplying water to the floral foam.
  11. 제7항에 있어서, The method of claim 7, wherein
    상기 용화시키는 단계는 0L·24D의 광주기 환경에서 수행되는 것을 특징으로 하는 장수하늘소의 인공증식방법.The lysing step is artificial proliferation of longevity sky cow, characterized in that carried out in a photoperiod environment of 0L · 24D.
  12. 참나무 톱밥 및 분말혼합물을 포함하는 장수하늘소 증식용 인공사료에 있어서, In the artificial feed for longevity sky cow growth comprising oak sawdust and powder mixture,
    상기 참나무 톱밥은 수침한 참나무를 50℃ 내지 60℃에서 마이크로 웨이브를 조사하고, 수분함량이 1% 내지 5%가 되도록 건조한 후 분쇄하여 분말화된 참나무 톱밥인 것을 특징으로 하는 장수하늘소 증식용 인공사료.The oak sawdust is an artificial feed for longevity sky cow growth, characterized in that the oak sawdust powdered by drying the soaked oak at 50 ℃ to 60 ℃ irradiated with microwave, dried to moisture content of 1% to 5% .
PCT/KR2013/004362 2012-05-23 2013-05-16 Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same WO2013176441A1 (en)

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