CN104430183B - Large-scale breeding and method for breeding in a kind of greater wax moth room - Google Patents
Large-scale breeding and method for breeding in a kind of greater wax moth room Download PDFInfo
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- CN104430183B CN104430183B CN201310416520.6A CN201310416520A CN104430183B CN 104430183 B CN104430183 B CN 104430183B CN 201310416520 A CN201310416520 A CN 201310416520A CN 104430183 B CN104430183 B CN 104430183B
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- 241000255896 Galleria mellonella Species 0.000 title claims abstract description 72
- 238000009395 breeding Methods 0.000 title claims abstract description 33
- 230000001488 breeding effect Effects 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 20
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 30
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 30
- 210000004681 ovum Anatomy 0.000 claims abstract description 30
- 238000012360 testing method Methods 0.000 claims abstract description 7
- 229920003023 plastic Polymers 0.000 claims description 27
- 239000004033 plastic Substances 0.000 claims description 21
- 235000012907 honey Nutrition 0.000 claims description 15
- 229920000742 Cotton Polymers 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 9
- 235000013305 food Nutrition 0.000 claims description 9
- 235000015099 wheat brans Nutrition 0.000 claims description 8
- 230000032683 aging Effects 0.000 claims description 7
- 239000002985 plastic film Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 4
- 238000009987 spinning Methods 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims 1
- 210000003205 muscle Anatomy 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 abstract description 10
- 241000382353 Pupa Species 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000010923 batch production Methods 0.000 abstract 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 235000013601 eggs Nutrition 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 235000013312 flour Nutrition 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 239000001993 wax Substances 0.000 description 3
- 241000256844 Apis mellifera Species 0.000 description 2
- 241000122111 Galleriinae Species 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 2
- 241000255893 Pyralidae Species 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 235000013871 bee wax Nutrition 0.000 description 2
- 239000012166 beeswax Substances 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 210000004894 snout Anatomy 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention belongs to resource insect indoor propagation and breeding technology field, it is related to large-scale breeding and method for breeding in a kind of greater wax moth room.The present invention is first to collect individual healthy and strong mature larva as introduces a collection, put it into pupate device container in pupate, greater wax moth pupa is taken out after one week to be put into the container with ovum collecting device, after one week, take out ovum collecting device, ovum is removed and is put into according to a certain percentage in raising container by feed and ovum, addition is needed when feed is consumed 90% or so.Removed when greater wax moth mature larva is climbed out of from feed, select it is big continue to breed as introduces a collection, remaining is put back in feed and raises or use or sell as test worm.Large-scale breeding it is an object of the invention to overcome greater wax moth is time-consuming, can not realize the problem of annual and batch production, there is provided a kind of indoor propagation and method for breeding that can produce greater wax moth in enormous quantities.
Description
Technical field
The invention belongs to resource insect indoor propagation and breeding technology field, it is related to large-scale breeding in a kind of greater wax moth room
And method for breeding.
Background technology
Greater wax moth belongs to Lepidoptera (Lepidoptera), Pyralidae (Pyralidae), Galleriinae (Galleriinae)
Insect.Greater wax moth larva is food with honeycomb, can destroy storage honeycomb, and can encroach on bee colony, also known as " nest worm, continuous worm, tunnel worm ".
But because greater wax moth growth cycle is short, foodstuff source be easy to get, it is easy to amount reproduction, be nematology, bacteriology, mycology, medical science,
Important experimental insect on the disciplinary studies such as agronomy；It is excellent bioreactor carrier in molecular biology research；
As excellent vector in the numerous research of expansion of natural enemy insect；The endotrophic composition of greater wax moth enriches, albumen and fat
Content is higher, is a kind of excellent living body bait of freshwater fish, birds, reptiles and amphibian animal.Furthermore, Zuo Zhefa
Existing fried greater wax moth larva is tasty, and a kind of light sweet taste is had more compared with other food insects, therefore greater wax moth is used as one kind
Insect food, there is value of exploiting and utilizing very much.
Just because of above-mentioned scientific research and economic worth, so the biology of greater wax moth, breeding technology etc. have more
Person is studied, and can find many research reports.So far, artificial feeding of the domestic and international researcher to greater wax moth is made
Numerous studies, related temperature, humidity, illumination condition determines substantially.It is external in terms of the man-made feeds research of greater wax moth
The report of Balazs was just seen early in 1958.At home, Zhang Gangying (1995) first Application feed formula：Milk powder, flour, wheat
Each 1 part of bran, beeswax, dusty yeast, bean powder, honey, glycerine and each 0.6 part of corn flour, have studied the indoor feeding technology of greater wax moth.
Pu Heng watersides etc. (2009) have studied DIFFERENT FEED formula to greater wax moth growth effect evaluation, find feed formula：Corn flour, milk
Powder, wheat bran, ferment, glycerine, honey are respectively 300,100,200,100,150,200g is to the feeding effect of greater wax moth larva
Preferably, output efficiency is high, low cost.Huang Chenghua etc. (2010) carries out screening test to greater wax moth man-made feeds, and overall merit is raised
Material formula is to the development of greater wax moth larval growth, pupa weight, egg laying amount and goes through the influence of phase, while taking into account the cost of feed formula, tries
Issuing after examination and approval now comparatively ideal greater wax moth feed formula is：Dusty yeast 5.40g, the milk powder 10.20g of glycerine 24.00, honey 11.76g, honeybee
Wax 16.68g, flour 51.96g.In terms of breeding apparatus, larva raising mainly uses Cans (Zhang Gangying, 1995), bottom
Face diameter 14cm, 10cm cylindrical plastics box (bear prolongs female etc., 2002) high, 25cm × 16cm × 8cm plastic lunch box (Zhao Jun,
2010) etc..Spawning device is used：Spawning bar (band of 15cm × 1cm red shrinkages) (Zhao Jun, 2010).Corrugated board
It is that people use more spawning and the material pupated.
My extensive indoor propagation and find during raising greater wax moth, in restriction greater wax moth room large-scale breeding and
The subject matter of raising is time-consuming, is embodied in four aspects：One be pupa collection it is more time-consuming.Greater wax moth is liked
Vigorously pupated in narrow space, and the hole of corrugated board is well suited for pupating, therefore, people like for corrugated board being put into feeding
Material surface, ripe pupa is to pierce hole to pupate.I also used the method to collect pupa, and discovery has 3 shortcomings：Corrugated board
It is put into after a period of time, can be spun by greater wax moth with the position that feed is contacted Nian Jie with feed, is not easily taken out；It is also immature
Larva is likely to pierce and eats up part pupa in cardboard；Cocoon is combined closely with corrugated board, is not easily taken out, somewhat firmly pupa
It is i.e. injured.Two be ovum collection it is more time-consuming.Greater wax moth likes spawning at narrow gap, and ovum is Nian Jie with attachment tight
Close, forefathers are laid eggs using spawning paper and corrugated board for greater wax moth, and 2 kinds of materials are entirely capable of meeting the spawning need of greater wax moth adult
Ask, but be difficult after greater wax moth is laid eggs thereon to remove on ovum, can be cut ovum and the scraps of paper for one piece with scissors, but compare expense
Work.Three is that the man-made feeds of greater wax moth are prepared and compare and take a lot of work.The feed formula of forefathers is observed, it is found that the formula species of feed is more,
Especially with the formula of beeswax, prepare and be even more trouble.In addition, winter temperature is low, greater wax moth development is slow, such as wants annual batch
Amount production, it is necessary to give insectary heating, this is undoubtedly the increase in foster worm cost in addition.
The content of the invention
It is an object of the invention to overcome the batch life that the large-scale breeding of greater wax moth is time-consuming, can not realize annual
The problem of product, there is provided a kind of indoor propagation of greater wax moth that can produce in enormous quantities and the method raised.
What the present invention was realized in：
The first collection in honeycomb or the commercially mature larva of greater wax moth, therefrom screen individual healthy and strong larva work
It is the introduces a collection of indoor propagation.Larva is put into the container with device of pupating and is pupated, the larva collected on the same day is put into same
In container, the greater wax moth pupated is taken out later within one week and be put into the container with ovum collecting device, when recording the emergence of greater wax moth
Between, after sprouting wings one week to greater wax moth, ovum collecting device is taken out, ovum is removed and is put into raising according to a certain percentage by feed and ovum
In container, irregularly the Expenditure Levels of observation feed, need addition when feed is consumed 90% or so.Treat greater wax moth it is aging from
Removed when being climbed out of in feed, select being put into the container with device of pupating for stalwartness and pupate as introduces a collection continuation breeding, other
Continue put back in feed raise or use or sell as test worm.
Above-described larvae pupation, the collection of ovum and larva raising container are the transparent circle of diameter 17cm, 25cm high
Mould batch can.
The circular hole that the above-described lid for raising container must beat some diameters 0.5cm is breathed freely, with one piece of ratio under lid
The slightly larger black cloth of lid.
Above-described device of pupating is the transparent plastic film of 1mm for thickness.
Above-described ovum collecting device is that thickness is the pearl cotton of 1mm.
Above-described feed formula is wheat bran：Honey paste：Dusty yeast is 5: 4: 1.
Above-described feed (g) is 1: 10 with the ratio that is put into of ovum (individual).
Specific breeding and method for breeding step are：
(1) collection of greater wax moth introduces a collection
Greater wax moth is the insect in honeycomb, and introduces a collection can be gathered from honeycomb.There is a small amount of big wax in the market
Snout moth's larva larva product is sold, and introduces a collection can also be obtained by buying.
(2) mature larva is pupated
The thick length and width of one piece of 1cm are into the transparent plastic film of 50cm to be put into transparent circular plastic tank, then will be aging
Larva is put into wherein, and mature larva can cocoon between the gauffer of film and pupate.
(3) collection of ovum
After one week, mature larva spinning spins, and cocoon can be now removed from film, and unification is put into transparent circular plastic tank
In, then one piece of the pearl cotton of 80cm*80cm is put into tank, ball and tied with rubber band before being put into.
(4) preparation of feed
Get out the raw materials such as wheat bran, honey paste, dusty yeast and plastic tub one.Wheat bran, dusty yeast are contained in 5: 1 ratio
Enter the honey paste that 4 parts are poured into after being well mixed in plastic tub, be well mixed.Raising newly hatched larvae will suitably reduce honey paste
Ratio, in case newly hatched larvae is dead by adhesion, instar larvae high will suitably increase the ratio of honey paste, to meet its nutrition need
Ask.
(5) raising of greater wax moth larva
The emergence time of greater wax moth is recorded, after sprouting wings one week to greater wax moth, pearl cotton is taken out, peel ovum above,
Ovum is put into raising container in feed and ovum in 1: 10 ratio, feed is taken food after egg hatching, irregularly observe feed
Expenditure Levels, need addition when feed is consumed 90% or so.Needs are drawn it is noted that tank mouth needs to beat some diameters
The circular hole of 0.5cm is breathed freely, and prevents larva from escaping with one block of black cloth more slightly larger than lid under lid.
(6) collection of mature larva
The greater wax moth according to said method raised, can climb out of when aging and be climbed at feed and upwards past tank mouth, now can be by stalwartness
Mature larva taking-up is pupated in being put into tank of pupating, and remaining continues to put back to and raised in feed or use or sell as test worm.
The advantage and good effect of the inventive method：
1st, large batch of greater wax moth can be at any time obtained, is used as test worm, life food or insect food etc..
2nd, due to greater wax moth taking food in feed and the habit very not moved, temperature is higher than room temperature in making feed.This hair
The bright transparent circular plastic tank for using, there is certain height, in the winter time can be by increasing the stocking density and plastics of greater wax moth
The mode of thickness increases the temperature in tank, makes the greater wax moth in winter can also normal growth without heating.
3rd, greater wax moth likes ovum to produce in gap, and the ovum viscosity given birth to is very strong, it is not easy to remove, the present invention is used
Pearl cotton knead after have the gaps of many suitable greater wax moths spawnings, most importantly, produce the ovum in the pearl cotton and be easy to
Just can be stripped.
4th, spinning is cocoond before greater wax moth is pupated, and cocoon is firmly adhered on attachment, and conventional method for breeding uses corrugation
Paper is as pupa collection device, and after greater wax moth is pupated in corrugated paper, and cocoon is very firm with what paper glued, removes highly difficult, is also easy to damage
Hinder polypide, be unfavorable for centralized collection.The present invention is to pupate device using film, it is easy to solution this problem, film very light
Sliding, after greater wax moth is pupated, cocoon is easy to remove.
5th, the development duration of the greater wax moth larva that the feed formula that the present invention is used is raised will be grown compared with the formula that forefathers use,
But as long as ensureing the usage amount of honey paste, the weight and Adult worms producting eggs amount of its greater wax moth mature larva raised do not have difference, but
This feed formulation is convenient, and low cost is more suitable for being used during large-scale breeding.
Brief description of the drawings
Fig. 1：It is the process chart of large-scale breeding and method for breeding in greater wax moth room of the present invention.
Specific embodiment
The inventive method is described in further detail by the following examples, but it is understood that the present invention does not receive this
A little contents are limited.
Embodiment：
(1) collection of greater wax moth introduces a collection
Greater wax moth is the insect in honeycomb, and introduces a collection can be gathered from honeycomb.There is a small amount of big wax in the market
Snout moth's larva larva product is sold, and introduces a collection can also be obtained by buying.
(2) mature larva is pupated
The thick length and width of one piece of 1cm are into the transparent plastic film of 50cm to be put into transparent circular plastic tank, then will be aging
Larva is put into wherein, and mature larva can cocoon between the gauffer of film and pupate.
(3) collection of ovum
After one week, mature larva spinning spins, and cocoon can be now removed from film, and unification is put into transparent circular plastic tank
In, then one piece of the pearl cotton of 80cm*80cm is put into tank, ball and tied with rubber band before being put into.
(4) preparation of feed
Get out the raw materials such as wheat bran, honey paste, dusty yeast and plastic tub one.Wheat bran, dusty yeast are contained in 5: 1 ratio
Enter the honey paste that 4 parts are poured into after being well mixed in plastic tub, be well mixed.Raising newly hatched larvae will suitably reduce honey paste
Ratio, in case newly hatched larvae is dead by adhesion, instar larvae high will suitably increase the ratio of honey paste, to meet its nutrition need
Ask.
(5) raising of greater wax moth larva
The emergence time of greater wax moth is recorded, after sprouting wings one week to greater wax moth, pearl cotton is taken out, peel ovum above,
Ovum is put into raising container in feed and ovum in 1: 10 ratio, feed is taken food after egg hatching, irregularly observe feed
Expenditure Levels, need addition when feed is consumed 90% or so.Needs are drawn it is noted that tank mouth needs to beat some diameters
The circular hole of 0.5cm is breathed freely, and prevents larva from escaping with one block of black cloth more slightly larger than lid under lid.
(6) collection of mature larva
The greater wax moth according to said method raised, can climb out of when aging and be climbed at feed and upwards past tank mouth, now can be by stalwartness
Mature larva taking-up is pupated in being put into tank of pupating, and remaining continues to put back to and raised in feed or use or sell as test worm.
Claims (5)
1. large-scale breeding and method for breeding in a kind of greater wax moth room, it is characterised in that：Concrete operation step is：
(1) collection of introduces a collection
Greater wax moth introduces a collection is gathered from honeycomb or obtained by purchase, introduces a collection is put into standby in transparent circular plastic tank；
(2) mature larva is pupated
It is the transparent plastic film gauffer that 1cm, length and width are 50cm by thickness, is put into transparent circular plastic tank, then will be aging
Larva is put into transparent circular plastic tank, is allowed mature larva to be cocoond between the gauffer of transparent plastic film and is pupated；
(3) collection of ovum
After one week, mature larva spinning is spun, and now cocoon is removed from transparent plastic film, and unification is put into transparent circular plastics
In tank, then one piece of the pearl cotton that thickness is 80cm for 1mm, length and width is put into tank, ball and rubber is used before being put into
Muscle is tied；
(4) raising of larva
The emergence time of greater wax moth is recorded, after sprouting wings one week to greater wax moth, pearl cotton is taken out, peel ovum above, will raised
Material is put into transparent circular plastic tank with ovum, and larva freely takes food feed after egg hatching, otherwise the consumption of routine observation feed
Situation, when feed is consumed 90%, it is necessary to add feed again；The black cloth that the lid lower section of tank mouth is matched somebody with somebody during hatching will be fixed,
Prevent larva from escaping；
(5) collection of mature larva
Larva can climb out of when aging and be climbed at feed and the tank mouth to transparent circular plastic tank, now take healthy and strong mature larva
Go out, carry out step (2) mature larva and pupate, remaining continues to raise or uses or sell as test worm.
2. large-scale breeding and method for breeding in a kind of greater wax moth room according to claim 1, it is characterised in that：Described
The thickness of transparent circular plastic tank is 1mm, a diameter of 17cm, a height of 25cm, and it is straight that the lid of transparent circular plastic tank is equipped with some
The ventilative circular hole of footpath 0.5cm, is furnished with one block of black cloth more slightly larger than lid in the lower section of lid.
3. large-scale breeding and method for breeding in a kind of greater wax moth room according to claim 1, it is characterised in that：Described
The weight ratio of feed is：Wheat bran: honey paste: feed yeast powder=5: 4: 1.
4. large-scale breeding and method for breeding in a kind of greater wax moth room according to claim 1, it is characterised in that：Described
Raising is put into 10, ovum by feed 1g.
5. large-scale breeding and method for breeding in a kind of greater wax moth room according to claim 3, it is characterised in that：Described
The compound method of feed：By in the ratio addition plastic tub of wheat bran, feed yeast powder by weight 5: 1, it is well mixed, Ran Houzai
4 parts of honey paste is added, is well mixed.
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| CN107182948A (en) * | 2017-06-12 | 2017-09-22 | 湖南工业大学 | Greater wax moth pilot scale culture new method |
| WO2019092417A1 (en) * | 2017-11-08 | 2019-05-16 | Zyzzle Limited | Method and apparatus for rearing small animals |
| CN108633845A (en) * | 2018-05-14 | 2018-10-12 | 北京安和亿泰生物工程技术有限公司 | A kind of cultural method of the cured snout moth's larva of efficient degradation plastics |
| CN108782470B (en) * | 2018-06-20 | 2020-03-24 | 广西壮族自治区林业科学研究院 | Greater wax moth egg collecting device and application |
| CN108925514A (en) * | 2018-06-28 | 2018-12-04 | 郑州伊美诺生物技术有限公司 | A kind of greater wax moth Laboratory Standard cultural method |
| CN112167181A (en) * | 2020-10-30 | 2021-01-05 | 新疆农业科学院土壤肥料与农业节水研究所(新疆维吾尔自治区新型肥料研究中心) | Breeding method for improving commodity value of greater wax moth |
| CN114698602A (en) * | 2022-03-30 | 2022-07-05 | 重庆市农业科学院 | Method for feeding corophium acherusicum by using galleria mellonella |
| CN114916505B (en) * | 2022-06-21 | 2024-07-23 | 新疆农业科学院土壤肥料与农业节水研究所(新疆维吾尔自治区新型肥料研究中心) | Population continuation research method for biodegradable farmland residual films of Chilo suppressalis |
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