CN108125073A - Corn borer meridic diet and preparation method thereof and method for breeding - Google Patents
Corn borer meridic diet and preparation method thereof and method for breeding Download PDFInfo
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- 239000004033 plastic Substances 0.000 claims description 7
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- 241000607479 Yersinia pestis Species 0.000 claims description 6
- 238000007726 management method Methods 0.000 claims description 6
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 5
- 206010000496 acne Diseases 0.000 claims description 5
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- 230000032669 eclosion Effects 0.000 claims description 4
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- 238000011161 development Methods 0.000 abstract description 5
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- Birds (AREA)
- Insects & Arthropods (AREA)
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Abstract
The invention discloses a kind of corn borer meridic diet and preparation method thereof and method for breeding, weight percent composition is as follows：Water 55~60%, soy meal 10~15%, corn flour 10~15%, dusty yeast 5~10%, white sugar 5~10%, Victoria C 0.30~0.35%, sorbic acid 0.30~0.35%, milled powders of cornstalk 5~10%, water soluble starch 1.0~1.5%, formaldehyde 0.010~0.015%.The corn borer meridic diet cost of the present invention is relatively low, feed raw material sources are wide, prepare the easy, meridic diet without agar, suitable for corn borer growth and development breeding, so as to fulfill the long-term continuously rearing of corn borer.Pass through cornstalk powder and the efficient combination of water soluble starch, instead of traditional agar powder corn borer larvae is raised as forming agent, its corn borer larvae survival rate, percentage of pupation, property ratio, average pupa weight, egg laying amount are above existing feed formula, and feed cost is made to reduce 1/3rd.
Description
Technical field
The invention belongs to insects to raise field, and in particular to a kind of meter of snout moth's larva meridic diet and preparation method thereof and raising side
Method.
Background technology
Corn borer survival ability is strong, and polypide is small, heavy, and feeding habits are miscellaneous, causes harm wide.In China, adaptability grade is wide, annual each
1-7 generations, which can occur, for ground differs, and is the important pests of upland grain, corn, millet, sorghum etc..Seriously affect the yield of corn
And quality, in recent years, due to corn borer research deepen continuously and the development of control of insect technology, need a large amount of physiological standard phases
Worm is tested to consistent corn borer, this promotes the fast development of artificial feeding corn borer technology, and as the base of corn borer research
One of this technology.The research of man-made feeds is now subjected to attention, also has developed many meridic diet formulas, raises each worm
The corn borer of state is used the integrated control of corn borer and the research of behavior science extensively.However it is sent out in the practice of mass rearing
Existing, many feeding formulas are all using agar as gelation substance, thus are inevitably present two problems：First, because feed
Middle agar value is high, causes feed cost higher and makes complicated；Second is that thermosol agar needs to consume energy cost expenses of labour
Thing, so as to seriously affect the expansion of raising scale.
Invention content
The technical problems to be solved by the invention are：How to provide that a kind of cost is relatively low, feed raw material sources are wide, prepares
The easy, meridic diet without agar, suitable for corn borer growth and development breeding, so as to fulfill the long-term continuously rearing of corn borer
Method for breeding, while the preparation method of corn borer meridic diet is provided.
The technical scheme is that：Corn borer meridic diet, weight percent composition are as follows：It is water 55~60%, big
Bean powder 10~15%, corn flour 10~15%, dusty yeast 5~10%, white sugar 5~10%, Victoria C 0.30~0.35%, sorbic acid
0.30~0.35%, milled powders of cornstalk 5~10%, water soluble starch 1.0~1.5%, formaldehyde 0.010~0.015%.
The preparation method of corn borer meridic diet, step are as follows：
(1) weigh soy meal in proportion, dusty yeast is put in high-pressure sterilizing pot, sterilize under the conditions of 121 DEG C of 1.0 temperature of pressure
It is taken out after 30 minutes spare；
(2) corn flour, white sugar, Victoria C, sorbic acid, milled powders of cornstalk, water soluble starch are weighed in proportion, with 20% -25%
Water stir evenly；It obtains mixing product.
(3) product that step (1) obtains is added in the product that step (2) obtains and adds formaldehyde, with remaining water
It stirs evenly, obtains man-made feeds.
The method that corn borer meridic diet raises corn borer, includes the following steps：
(1) the hatching management method of ovum：Moistening curl-paper box is placed with by produce that the fresh-keeping bag webs concentration that there are pieces of an egg is placed on sterilizing
In, incubator is then placed in, 27.5~28.5 DEG C of hatchings are kept, when pieces of an egg are by milky white-yellowish-light brown-blackhead, with 2%
The formaldehyde of volume by volume concentration impregnates 10 minutes plus the sodium hypochlorite of 2% volume by volume concentration to be sterilized, and is then taken out and is rinsed with clear water,
Again with continuing to be put into incubator and hatch after disinfection roll paper suck dry moisture；
(2) larva raising method：Corn borer meridic diet is fitted into feed bottle, squab larva sweeping gently
It is sealed onto feed, then with sealed membrane, the feed bottle after sealing is placed in culture of pests case, keep constant temperature 28 DEG C~30
DEG C, mature larva after larva is aging, is put into the plastics for folding paper slip by relative humidity 70%~80%, 15-16h of illumination
It pupates in box；
(3) management after pupating：Pupa is picked out concentration to be placed in the culture dish of wet foam and freshness protection package disk, per ware
90~110 are put, culture dish is put into dependent insect cage, 28 DEG C~30 DEG C of holding, relative humidity 80%~90%, cage periphery wet hair
Towel moults towel twice daily；
(4) imago breeding method：When there is adult eclosion in cage, be put into 2-3 fill 8-12% hydromel 10-
15ml centrifuge tubes are clogged with disinfection cotton, are drunk for adult, the freshness protection package cut apart with a knife or scissors is opened on cage upper berth one, are made corn borer oviposition and are situated between
Matter changes 1 fresh-keeping bag webs, changes towel twice, and takes out dead moth daily, is kept for 28 DEG C~30 DEG C, relative humidity 88%~92%.
Further, in step (1), the fresh-keeping bag webs concentration that production there are pieces of an egg is placed on being placed with for sterilizing and moistens roll paper specification
In plastic casing for the ㎝ of the ㎝ of 22 ㎝ × 13 × 6, be then placed in incubator, keep 28 DEG C of hatchings, treat pieces of an egg by it is milky white-yellowish-
During light brown-blackhead, impregnated 10 minutes and sterilized plus the sodium hypochlorite of 2% volume by volume concentration with the formaldehyde of 2% volume by volume concentration,
It then takes out and is rinsed with clear water, then hatched with continuing to be put into incubator after disinfection roll paper suck dry moisture.
Further, in step (2), corn borer meridic diet is fitted into feed bottle, squab larva gently
Sweep on feed, then sealed with sealed membrane, the feed bottle after sealing is placed in culture of pests case, keep 28 DEG C of constant temperature~
30 DEG C, relative humidity 70%~80%, illumination 16h after larva is aging, mature larva is put into and is folded with the specification of paper slip and is
It pupates in the plastic casing of the ㎝ of the ㎝ of 22 ㎝ × 13 × 6.
Further, in step (3), pupa is picked out into concentration and is placed in the culture dish of wet foam and freshness protection package disk,
100 are put per ware, culture dish is put into dependent insect cage, 28 DEG C~30 DEG C of holding, relative humidity 80%~90%, cage periphery wet hair
Towel moults towel twice daily.Change the fresh-keeping bag webs of oviposition daily after laying eggs.
Water meets requirement and adaptation corn borer larvae biting mouthparts feeding of the corn borer larvae to moisture in the present invention
Needs, the source of water is not particularly limited.Soy meal is soybean purchased in market, meets corn borer to protein, aliphatic acid, also
Cholesterol necessary to insect growth, the needs of the nutriments such as tocopherol and various vitamins.Corn flour is commercially available corn, processing
It crushes, grain size is 80-100 mesh, provides needs of the corn borer larvae to protein, fat.Dusty yeast is Angel activity bread ferment
Female powder, meet corn borer larvae to protein, nucleic acid, carbohydrate needs.White sugar is white sugar purchased in market, is the master of biological energy i (in vivo)
The precursor of source and lipid and nonessential amino acid is wanted, meets needs and one kind of the corn borer larvae to carbohydrate
Feeding encourages substance.Vitamin C, nutritional ingredient or a kind of feeding stimulant necessary to be corn borer larvae growth.Sorbic acid
For feed anticorrosion.Cornstalk powder picks up from the cornstalk of the harvest time of no chemical contamination in field, removes blade, leaf sheath, powder
Broken to form, grain size is 30-40 mesh.Water soluble starch plays certain freezing action, keeps moisture in feed, meets entire corn borer
Requirement of the larval growth phase to high humility feed.
Compared with prior art, the invention has the advantages that：
The present invention corn borer meridic diet cost is relatively low, feed raw material sources are wide, it is easy to prepare, without agar half
Man-made feeds, suitable for corn borer growth and development breeding, so as to fulfill the long-term continuously rearing of corn borer.Pass through cornstalk powder and water
The efficient combination of soluble starch raises corn borer larvae instead of traditional agar powder, and corn borer larvae survival rate is pupated
Rate, property ratio, average pupa weight, egg laying amount are above existing feed formula, and feed cost is made to reduce 1/3rd.
Specific embodiment
Embodiment 1：
(1) component of following weight is weighed：700 grams of water；120 grams of soy meal；154 grams of corn flour；72 grams of dusty yeast；In vain
60 grams of sugar；4 grams of vitamin C；4 grams of sorbic acid；1.6 milliliters of formaldehyde；56 grams of cornstalk powder；24 grams of water soluble starch.
(2) preparation method of corn borer larvae man-made feeds, includes the following steps：
1st, by soy meal, dusty yeast in pressure 1.0,121 DEG C of sterilizings, 30 minutes materials to be sterilized.
2nd, by the material of the sterilizing obtained in above-mentioned steps and corn flour, white sugar, vitamin C, sorbic acid, cornstalk powder,
Water soluble starch and 20% water stir.
3rd, the product that step (1) obtains is added in the product that step (2) obtains and adds formaldehyde, stirred with remaining water
It mixes uniformly, obtains man-made feeds.
Embodiment 2：
(1) component of following weight is weighed：650 grams of water；120 grams of soy meal；154 grams of corn flour；72 grams of dusty yeast；In vain
60 grams of sugar；4 grams of vitamin C；4 grams of sorbic acid；1.6 milliliters of formaldehyde；60 grams of cornstalk powder；20 grams of water soluble starch.
(2) preparation method of corn borer larvae man-made feeds, includes the following steps：
1st, by soy meal, dusty yeast in pressure 1.0,121 DEG C of sterilizings, 30 minutes materials to be sterilized.
2nd, by the material of the sterilizing obtained in above-mentioned steps and corn flour, white sugar, vitamin C, sorbic acid, cornstalk powder,
Water soluble starch and 25% water stir.
3rd, the product that step (1) obtains is added in the product that step (2) obtains and adds formaldehyde, stirred with remaining water
It mixes uniformly, obtains man-made feeds.
Embodiment 3：
(1) component of following weight is weighed：700 grams of water；120 grams of soy meal；154 grams of corn flour；72 grams of dusty yeast；In vain
60 grams of sugar；4 grams of vitamin C；4 grams of sorbic acid；1.6 milliliters of formaldehyde；64 grams of cornstalk powder；16 grams of water soluble starch.
(2) preparation method of corn borer larvae man-made feeds, includes the following steps：
1st, by soy meal, dusty yeast in pressure 1.0,121 DEG C of sterilizings, 30 minutes materials to be sterilized.
2nd, by the material of the sterilizing obtained in above-mentioned steps and corn flour, white sugar, vitamin C, sorbic acid, cornstalk powder,
Water soluble starch and 23% water stir.
3rd, the product that step (1) obtains is added in the product that step (2) obtains and adds formaldehyde, stirred with remaining water
It mixes uniformly, obtains man-made feeds.
Comparative example 1：New No. 7 feed formulas are formulated with traditional corn borer meridic diet, with reference to new No. seven feed formulas
Preparation method manufacturing artificial feed as a control group, the formula of new No. seven feeds：120 grams of soy meal, 144 grams of corn flour, ferment
72 grams of female powder, 60 grams of white sugar, 4 grams of vitamin C, 4 grams of sorbic acid, 1.6 milliliters of formaldehyde, 1.2 grams of erythromycin, 1000 grams of water.
With the corn borer introduces a collection in 28 generation of laboratory rearing under same breeding facility and equal conditions, with embodiment 1-
3, the man-made feeds that comparative example 1 makes raise Ostrinia furnacalis respectively, and 100-153, snout moth's larva ovum is connect per container, and often processing sets 5
It repeats, entire experiment is repeated 3 times, and with for examination snout moth's larva ovum 8000-10000, calculates its survival rate and percentage of pupation.Institute is repeated by each
Obtained corn borer pupa all differentiates male and female, therefrom takes 100 pairs or 200 pairs of male and female pupa at random, is put into homemade adult eclosion production
In ovum cage, placed freshness protection package (cutting apart with a knife or scissors) one above cage, when production money ovum of sprouting wings starts, the block number of record oviposition daily calculates
Single female eggs on average amount is finally commented with larva survival rate, percentage of pupation, property ratio, male and female pupa weight and single female 5 index comprehensives of egg laying amount
The feeding effect of valency each group feed.
Table 1：Each group feed raises the effect of Ostrinia furnacalis：
It is learnt by 1 result of table, the method for the present invention corn borer meridic diet larva survival rate 82.7 ± 1.4, percentage of pupation
93.3 ± 0.7, property is than 1:113 ± 0.05, female average pupa weight is 137.3 ± 2.7, and hero is 84.8 ± 1.1, egg laying amount for 11.8 ±
1.8.By cornstalk powder and the efficient combination of water soluble starch, corn borer larvae is raised instead of traditional agar powder, it is beautiful
Rice snout moth's larva larva survival rate, percentage of pupation, property ratio, average pupa weight, egg laying amount are above comparative example 1 (new No. 7 feed formulas), are sent out with this
Bright method has continuously raised 28 generation of corn borer.Feed cost is made to reduce 1/3rd.
The method that corn borer meridic diet raises corn borer, includes the following steps：
(1) the hatching management method of ovum：Moistening curl-paper box is placed with by produce that the fresh-keeping bag webs concentration that there are pieces of an egg is placed on sterilizing
In, incubator is then placed in, 27.5~28.5 DEG C of hatchings are kept, when pieces of an egg are by milky white-yellowish-light brown-blackhead, with 2%
The formaldehyde of volume by volume concentration impregnates 10 minutes plus the sodium hypochlorite of 2% volume by volume concentration to be sterilized, and is then taken out and is rinsed with clear water,
Again with continuing to be put into incubator and hatch after disinfection roll paper suck dry moisture；
(2) larva raising method：Corn borer meridic diet is fitted into feed bottle, squab larva sweeping gently
It is sealed onto feed, then with sealed membrane, the feed bottle after sealing is placed in culture of pests case, keep constant temperature 28 DEG C~30
DEG C, mature larva after larva is aging, is put into the plastics for folding paper slip by relative humidity 70%~80%, 15-16h of illumination
It pupates in box；
(3) management after pupating：Pupa is picked out concentration to be placed in the culture dish of wet foam and freshness protection package disk, per ware
90~110 are put, culture dish is put into dependent insect cage, 28 DEG C~30 DEG C of holding, relative humidity 80%~90%, cage periphery wet hair
Towel moults towel twice daily；
(4) imago breeding method：When there is adult eclosion in cage, be put into 2-3 fill 8-12% hydromel 10-
15ml centrifuge tubes are clogged with disinfection cotton, are drunk for adult, the freshness protection package cut apart with a knife or scissors is opened on cage upper berth one, are made corn borer oviposition and are situated between
Matter changes 1 freshness protection package and collects pieces of an egg, changes towel twice, and take out dead moth, kept for 28 DEG C~30 DEG C, relative humidity 88% daily
~92%.
Embodiment described above only expresses the specific embodiment of the application, and description is more specific and detailed, but simultaneously
Cannot the limitation to the application protection domain therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, under the premise of technical scheme design is not departed from, various modifications and improvements can be made, these belong to this
The protection domain of application.
Claims (6)
1. corn borer meridic diet, which is characterized in that weight percent composition is as follows：Water 55~60%, soy meal 10~
15%th, corn flour 10~15%, dusty yeast 5~10%, white sugar 5~10%, Victoria C 0.30~0.35%, sorbic acid 0.30~
0.35%th, milled powders of cornstalk 5~10%, water soluble starch 1.0~1.5%, formaldehyde 0.010~0.015%.
2. the preparation method of corn borer meridic diet according to claim 1, which is characterized in that step is as follows：
(1) weigh soy meal in proportion, dusty yeast is put in high-pressure sterilizing pot, sterilize 30 points under the conditions of 121 DEG C of 1.0 temperature of pressure
It is taken out after clock spare；
(2) corn flour, white sugar, Victoria C, sorbic acid, milled powders of cornstalk, water soluble starch are weighed in proportion, with 20% -25% water
It stirs evenly；It obtains
(3) product that step (1) obtains is added in the product that step (2) obtains and adds formaldehyde, stirred with remaining water
Uniformly, man-made feeds are obtained.
3. using the method for corn borer meridic diet described in claim 1 raising corn borer, which is characterized in that including as follows
Step：
(1) the hatching management method of ovum：The fresh-keeping bag webs concentration that production there are pieces of an egg is placed on being placed with for sterilizing to moisten in curl-paper box, so
After be put into incubator, 27.5~28.5 DEG C of hatchings are kept, when pieces of an egg are by milky white-yellowish-light brown-blackhead, with 2% volume
The formaldehyde of specific concentration impregnates 10 minutes plus the sodium hypochlorite of 2% volume by volume concentration to be sterilized, and is then taken out and is rinsed with clear water, then uses
Continue to be put into incubator after disinfection roll paper suck dry moisture and hatch；
(2) larva raising method：Corn borer meridic diet is fitted into feed bottle, squab larva gently sweep to feeding
It on material, then is sealed with sealed membrane, the feed bottle after sealing is placed in culture of pests case, keep 28 DEG C~30 DEG C of constant temperature, phase
To humidity 70%~80%, after larva is aging, mature larva is put into the plastic casing for folding paper slip by 15-16h of illumination
It pupates；
(3) management after pupating：Pupa is picked out concentration to be placed in the culture dish of wet foam and freshness protection package disk, 90 are put per ware
~110, culture dish is put into dependent insect cage, 28 DEG C~30 DEG C of holding, relative humidity 80%~90%, cage periphery wet towel, often
It moults towel twice；
(4) imago breeding method：When there is adult eclosion in cage, it is put into 2-3 10-15ml for filling 8-12% hydromel
Centrifuge tube is clogged with disinfection cotton, is drunk for adult, the freshness protection package cut apart with a knife or scissors is opened on cage upper berth one, makees corn borer oviposition medium, often
It changes 1 freshness protection package and collects pieces of an egg, changes towel twice, and takes out dead moth, 28 DEG C~30 DEG C of holding, and relative humidity 88%~
92%.
4. the method for corn borer meridic diet raising corn borer according to claim 3, which is characterized in that step (1)
In, it is placed with plastics of the moistening roll paper specification for the ㎝ of the ㎝ of 22 ㎝ × 13 × 6 by produce that the fresh-keeping bag webs concentration that there are pieces of an egg is placed on sterilizing
In box, incubator is then placed in, 28 DEG C of hatchings are kept, when pieces of an egg are by milky white-yellowish-light brown-blackhead, with 2% volume ratio
The formaldehyde of concentration impregnates 10 minutes plus the sodium hypochlorite of 2% volume by volume concentration to be sterilized, and is then taken out and is rinsed with clear water, then with disappearing
Continue to be put into incubator after malicious roll paper suck dry moisture and hatch.
5. the method for corn borer meridic diet raising corn borer according to claim 3, which is characterized in that step (2)
In, corn borer meridic diet is fitted into feed bottle, squab larva sweeping on feed gently, then is sealed with sealed membrane
Mouthful, the feed bottle after sealing is placed in culture of pests case, 28 DEG C~30 DEG C of constant temperature of holding, relative humidity 70%~80%,
Mature larva after larva is aging, is put into the plastic casing for being folded with the specification of paper slip as the ㎝ of the ㎝ of 22 ㎝ × 13 × 6 by illumination 16h
In, raising is to pupating.
6. the method for corn borer meridic diet raising corn borer according to claim 3, which is characterized in that step (3)
In, pupa is picked out into concentration and is placed in the culture dish of wet foam and freshness protection package disk, puts 100 per ware, culture dish is put into foster
In worm cage, kept for 28 DEG C~30 DEG C, relative humidity 80%~90%, cage periphery wet towel moults towel twice daily.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108813114A (en) * | 2018-06-26 | 2018-11-16 | 河南省农业科学院植物保护研究所 | Corn borer larvae man-made feeds and its preparation method and application |
| CN109156671A (en) * | 2018-10-17 | 2019-01-08 | 溧阳益植生物技术有限公司 | A kind of feed and preparation method thereof of artificial feeding corn borer |
| CN109430178A (en) * | 2018-12-28 | 2019-03-08 | 中国农业科学院植物保护研究所 | A method of extending Candle-sticks stinkbug Life of Adult |
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| CN108813114A (en) * | 2018-06-26 | 2018-11-16 | 河南省农业科学院植物保护研究所 | Corn borer larvae man-made feeds and its preparation method and application |
| CN108813114B (en) * | 2018-06-26 | 2021-07-30 | 河南省农业科学院植物保护研究所 | Artificial feed for corn borer larvae as well as preparation method and application thereof |
| CN109156671A (en) * | 2018-10-17 | 2019-01-08 | 溧阳益植生物技术有限公司 | A kind of feed and preparation method thereof of artificial feeding corn borer |
| CN109430178A (en) * | 2018-12-28 | 2019-03-08 | 中国农业科学院植物保护研究所 | A method of extending Candle-sticks stinkbug Life of Adult |
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