CN109430178A - A method of extending Candle-sticks stinkbug Life of Adult - Google Patents
A method of extending Candle-sticks stinkbug Life of Adult Download PDFInfo
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- CN109430178A CN109430178A CN201811619349.8A CN201811619349A CN109430178A CN 109430178 A CN109430178 A CN 109430178A CN 201811619349 A CN201811619349 A CN 201811619349A CN 109430178 A CN109430178 A CN 109430178A
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- 241000578422 Graphosoma lineatum Species 0.000 title claims abstract description 132
- 238000000034 method Methods 0.000 title claims abstract description 34
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 76
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 76
- 210000004681 ovum Anatomy 0.000 claims abstract description 76
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 58
- 239000008367 deionised water Substances 0.000 claims abstract description 35
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 35
- 230000009514 concussion Effects 0.000 claims abstract description 32
- 238000012545 processing Methods 0.000 claims abstract description 24
- 230000001954 sterilising effect Effects 0.000 claims abstract description 22
- 230000012447 hatching Effects 0.000 claims abstract description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 36
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 20
- 238000009395 breeding Methods 0.000 claims description 10
- 238000005119 centrifugation Methods 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 6
- 241000819999 Nymphes Species 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 230000010355 oscillation Effects 0.000 claims description 5
- 241000409991 Mythimna separata Species 0.000 claims description 2
- 241000382353 Pupa Species 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 7
- 206010010254 Concussion Diseases 0.000 description 25
- 235000013601 eggs Nutrition 0.000 description 13
- 235000019441 ethanol Nutrition 0.000 description 11
- 241000238631 Hexapoda Species 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 6
- 239000007921 spray Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000017448 oviposition Effects 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241001124076 Aphididae Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001367013 Ctenoplusia agnata Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 206010021033 Hypomenorrhoea Diseases 0.000 description 1
- 241000254043 Melolonthinae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 210000000554 iris Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of method for extending Candle-sticks stinkbug Life of Adult, include the following steps: that 1) the new Candle-sticks stinkbug ovum that produces is put into centrifuge tube；2) after sterile deionized water being added into the centrifuge tube in step 1), centrifuge tube is put into oscillator and is at least shaken processing three times；3) after sterile deionized water being added to the centrifuge tube in step 2), centrifuge tube is put into the concussion processing carried out in oscillator；4) into the centrifuge tube in step 3) after sterile deionized water, centrifuge tube is put into oscillator and carries out concussion processing；5) by Candle-sticks stinkbug ovum obtained in step 4) with sterile deionized water be rinsed it is clean after, with blotting surface moisture in sterilizing filter paper.The Candle-sticks stinkbug ovum that the present invention is handled by above-mentioned experimental procedure, after hatching forms adult, under the normal husbandry conditions of laboratory, Life of Adult is obviously increased, so that the expansion for helping to improve Candle-sticks stinkbug population is numerous, saves economy, time and the cost of labor of the raising of Candle-sticks stinkbug.
Description
Technical field
The present invention relates to insect artificial feeding field more particularly to a kind of methods for extending Candle-sticks stinkbug Life of Adult.
Background technique
The use of long-term chemical pesticide seriously threatens China's food and ecological safety, has also greatly affected state
People's health, the prevention and treatment problem of crop pests is particularly important here.It can for the biological control of core currently, being used with natural enemy insect
The effectively quantity of control pest, reduces the use of pesticide.And the numerous work of interior expansion for carrying out natural enemy insect is that it is applied to nocuousness
The primary work of biological control.Candle-sticks stinkbug belongs to Semiptera Pentatomiddae, is a kind of wide natural enemy insect of predation range, can prey on it is chrysomelid,
The larva or adult of more than 40 agriculture and forestry injurious insect such as bollworm, three-spotted plusia, slug moth, chafer, aphid.Natural enemy insect is by indoor
Mostly after generation raising, it often will appear the lost of life, egg laying amount decline, ovum reduces etc. again to be unfavorable for its growth and development and expand numerous
Phenomenon.And the adult lost of life can greatly influence the sum of predation pest after its release, also influence its egg laying amount, how to prolong
Long Candle-sticks stinkbug Life of Adult becomes the problem of Candle-sticks stinkbug breeding process is highly paid close attention to.
Summary of the invention
Compared with prior art, the present invention provides a kind of methods for extending Candle-sticks stinkbug Life of Adult, to solve the prior art
At least one of problem technical problem.
The technical scheme to solve the above technical problems is that a kind of method for extending Candle-sticks stinkbug Life of Adult, including
Following steps:
1) the new Candle-sticks stinkbug ovum that produces is put into centrifuge tube；
2) after sterile deionized water being added into the centrifuge tube in step 1), centrifuge tube is put into oscillator and is carried out at least
Concussion is handled three times；2.5~3.5min of centrifuge tube is stood between the adjacent concussion processing described twice；It then will be in centrifuge tube
Sterile deionized water is sucked out；
3) after being rinsed Candle-sticks stinkbug ovum obtained in step 2) completely with sterile deionized water, Candle-sticks is blotted with sterilizing filter paper
Stinkbug ovum surface moisture.
Based on the above technical solution, the present invention can also be improved as follows:
Preferably, the volume that Candle-sticks stinkbug ovum is newly produced described in step 1) accounts for the 1/3~2/5 of centrifugation pipe volume；In step 2), to
After the sterile deionized water for not crossing Candle-sticks stinkbug ovum is added in the centrifuge tube in step 1), centrifuge tube is put into oscillator and is carried out
At least concussion processing is every time that 500-1000rpm shakes 13~17s with revolving speed three times, stood between adjacent concussion twice processing from
2.5~3.5min of heart pipe.
Preferably, in step 2), after the sterile deionized water in the centrifuge tube is sucked out, then to the centrifugation in step 2)
Pipe, which is added, does not cross the volumetric concentration of Candle-sticks stinkbug ovum centrifuge tube to be put into 13~17s of progress in oscillator, is turned after 4% formalin
The concussion that speed is 500-1000rpm is handled, and after standing 4~5min, the formalin in the centrifuge tube is sucked out.
Preferably, in step 2), after the sterile deionized water in the centrifuge tube is sucked out, then to the centrifugation in step 2)
Pipe, which is added, does not cross the volumetric concentration of Candle-sticks stinkbug ovum centrifuge tube to be put into 13~17s of progress in oscillator, is turned after 75% ethanol solution
The concussion that speed is 500-1000rpm is handled, and after standing 4~5min, the ethanol solution in the centrifuge tube is sucked out.
Preferably, in step 3), after Candle-sticks stinkbug ovum obtained in step 2) is rinsed completely with sterile deionized water, then
Candle-sticks stinkbug oophyte is placed in sterilizing filter paper and blots surface moisture.
Preferably, the method for extending Candle-sticks stinkbug Life of Adult further includes step 4), by Candle-sticks stinkbug ovum obtained in step 3)
It is uniformly placed in sterilizes culture dish, the temperature that culture environment is arranged is 25~27 DEG C, humidity is 60~70%, the photoperiod is
15L:9D~17L:7D；It is placed with the sterilizing filter paper for having sprayed sterile purified water in the culture dish, sprays one into culture dish daily
Secondary sterile purified water, until Candle-sticks stinkbug egg hatching.
Preferably, the Candle-sticks stinkbug nymph after hatching obtained in step 4) is subjected to laboratory conventinal breeding, the laboratory
Conventinal breeding method includes feeding distilled water and tussah chrysalis and/or mythimna separata.
Preferably, the photoperiod of the environment of culture Candle-sticks stinkbug ovum is 16L:8D in step 4).
Preferably, the oscillator of step 2) is turbula shaker；The tool that the reagent in centrifuge tube is sucked out in step 2)
For pipette.
Preferably, 3~5 grams of new Candle-sticks stinkbug ovum that produce are put into 50ml centrifuge tube by step 1)；Centrifuge tube is put in step 2)
Entering the time that oscillator is shaken is 15s；The revolving speed of oscillator is 700~800rpm in step 2).
In conclusion the beneficial effects of the present invention are: the Candle-sticks stinkbug ovum handled by above-mentioned experimental procedure, hatching formed at
After worm, under the normal husbandry conditions of laboratory, Life of Adult is obviously increased, so that the expansion for improving Candle-sticks stinkbug population is numerous, saves Candle-sticks
Economy, time and the cost of labor of stinkbug raising.
Specific embodiment
Principles and features of the present invention are described with reference to embodiments, the given examples are served only to explain the present invention,
It is not intended to limit the scope of the present invention.
The present invention relates to a kind of method for extending Candle-sticks stinkbug Life of Adult, include the following steps: 1) to be put into the new Candle-sticks stinkbug ovum that produces
Into centrifuge tube；2) after sterile deionized water being added into the centrifuge tube in step 1), the centrifuge tube is put into concussion
Processing is at least shaken in device three times；2.5~3.5min of centrifuge tube is stood between the adjacent concussion processing described twice；Then will
Sterile deionized water in the centrifuge tube is sucked out；3) Candle-sticks stinkbug ovum obtained in step 2) is rushed with sterile deionized water
After wash clean, Candle-sticks stinkbug ovum surface moisture is blotted with sterilizing filter paper.The Candle-sticks stinkbug ovum that the present invention is handled by above-mentioned experimental procedure, is being incubated
After change forms adult, under the normal husbandry conditions of laboratory, Life of Adult is obviously increased, to help to improve Candle-sticks stinkbug population
Expand it is numerous, save Candle-sticks stinkbug raising economy, time and cost of labor.
In some embodiments, a method of extending Candle-sticks stinkbug Life of Adult, include the following steps: 1) will newly produce Candle-sticks stinkbug
Ovum is put into centrifuge tube；The new volume for producing Candle-sticks stinkbug ovum accounts for the 1/3~2/5 of centrifugation pipe volume；2) to the centrifuge tube in step 1)
After middle addition does not cross Candle-sticks stinkbug ovum sterile deionized water, centrifuge tube is put into oscillator and carries out the processing of concussion three times, every time to turn
Speed is that 500-1000rpm shakes 13~17s, stands 2.5~3.5min of centrifuge tube between the adjacent processing of concussion twice；It then will be from
Sterile deionized water in heart pipe is sucked out；It, will after being 4% formalin to the volumetric concentration that Candle-sticks stinkbug ovum is not crossed in centrifuge tube addition again
Centrifuge tube is put into 13~17s of progress in oscillator, and the concussion that revolving speed is 500-1000rpm is handled, after standing 4~5min, then
Formalin in the centrifuge tube is sucked out；3) Candle-sticks stinkbug ovum obtained in step 2) is rinsed with sterile deionized water dry
After net, then Candle-sticks stinkbug oophyte is placed in sterilizing filter paper and blots surface moisture；4) Candle-sticks stinkbug ovum obtained in step 3) is uniform
It is placed in sterilizes culture dish, the temperature that culture environment is arranged is 25~27 DEG C, humidity is 60~70%, photoperiod 15L:9D
~17L:7D；It is placed with the sterilizing filter paper for having sprayed sterile purified water in the culture dish, sprays primary sterilizing into culture dish daily
Distilled water, until Candle-sticks stinkbug egg hatching.The new Candle-sticks stinkbug ovum that produces is generally the oophyte within 12 to 24 hours postpartum.
In at least one embodiment, a method of extending Candle-sticks stinkbug Life of Adult, include the following steps: 1) will newly produce
Candle-sticks stinkbug ovum is put into centrifuge tube；The new volume for producing Candle-sticks stinkbug ovum accounts for the 1/3~2/5 of centrifugation pipe volume；2) in step 1) from
After the sterile deionized water for not crossing Candle-sticks stinkbug ovum is added in heart pipe, centrifuge tube is put into oscillator and carries out the processing of concussion three times, often
It is secondary that 13~17s is shaken for 500-1000rpm with revolving speed, 2.5~3.5min of centrifuge tube is stood between the adjacent processing of concussion twice；
Then the sterile deionized water in centrifuge tube is sucked out；Being added again to centrifuge tube and not crossing the volumetric concentration of Candle-sticks stinkbug ovum is 75% ethyl alcohol
After solution, centrifuge tube is put into 13~17s of progress in oscillator, the concussion that revolving speed is 500-1000rpm is handled, standing 4~
After 5min, then the ethanol solution in the centrifuge tube is sucked out；3) by the sterilizing deionization of Candle-sticks stinkbug ovum obtained in step 2)
After water is rinsed completely, then Candle-sticks stinkbug oophyte is placed in sterilizing filter paper and blots surface moisture；It 4) will be obtained in step 3)
Candle-sticks stinkbug ovum is uniformly placed in sterilizes culture dish, and the temperature that culture environment is arranged is 25~27 DEG C, humidity is 60~70%, light
Period is 15L:9D~17L:7D；The sterilizing filter paper for having sprayed sterile purified water is placed in the culture dish, daily to culture dish
Sterile purified water of interior spray, until Candle-sticks stinkbug egg hatching.The new Candle-sticks stinkbug ovum that produces is generally the oophyte within 12 to 24 hours postpartum.
In the method for above-mentioned extension Candle-sticks stinkbug Life of Adult, the volume that Candle-sticks stinkbug ovum is newly produced in step 1) accounts for centrifugation pipe volume
1/3~2/5, it is ensured that the maximum quantity of the Candle-sticks stinkbug ovum of single treatment improves treatment effeciency；
In step 2), the ethanol solution that volumetric concentration is 75% is added into centrifuge tube, for carrying out to the stinkbug ovum surface Candle-sticks
Disinfection, since excessive ethanol solution may cause the damage to Candle-sticks stinkbug ovum, the ethanol solution being added did not had Candle-sticks stinkbug ovum i.e.
Can, the application does not do excessive limitation to the ethanol solution amount that centrifuge tube is added.Centrifuge tube is put into oscillator and is carried out at concussion
Reason can effectively facilitate oophyte surface and come into full contact with reagent, and 500~1000rpm revolving speed is selected in method, strong in this concussion
Oophyte processing is carried out in degree range can achieve good cleaning effect, while oophyte will not be made to rupture；Concussion duration is set every time
It is set to 13~17s, while repeating repeatedly to shake, can achieve preferably cleaning effect, effectively avoids the occurrence of and is located at centrifugation
Ovum above pipe is cleaned, and the ovum of bottom does not reach cleaning effect；It is being arranged one section between processing per concussion twice
The standing of time effectively avoids shaking for a long time and brings mechanical damage to oophyte.
In step 2), the formalin that volumetric concentration is 4% is added into centrifuge tube, for disappearing to Candle-sticks stinkbug ovum surface
Poison, since excessive formalin may cause the damage to Candle-sticks stinkbug ovum, the formalin being added there was not Candle-sticks stinkbug ovum,
The application does not do excessive limitation to the formalin amount that centrifuge tube is added.Centrifuge tube is put into oscillator carry out concussion processing can
It is come into full contact with effectively facilitating oophyte surface with reagent, 500~1000rpm revolving speed is selected in method, in this impact strength model
Enclosing interior progress oophyte processing can achieve good cleaning effect, while oophyte will not be made to rupture；Concussion duration is set as 13~
17s can preferably clean oophyte；The standing of a period of time is set after earthquake, effectively restores concussion and machine is brought to oophyte
Tool damage.
In step 3), by Candle-sticks stinkbug ovum obtained in step 2) with sterile deionized water be rinsed it is clean after, then by Candle-sticks stinkbug
Oophyte, which is placed in sterilizing filter paper, blots surface moisture；Oophyte is rinsed with sterile deionized water again, can be removed residual
Deionized water, ethanol solution or the methanol solution of the carrying pollution biological impurities stayed；
In step 4), Candle-sticks stinkbug ovum obtained in step 3) is uniformly placed in sterilizes culture dish, culture environment is set
Temperature be 25~27 DEG C, humidity is 60~70%, the photoperiod is 15L:9D~17L:7D；It is placed with and sprays in the culture dish
The sterilizing filter paper of sterile purified water, sprays a sterile purified water into culture dish daily, until Candle-sticks stinkbug egg hatching.
Multiple washing, concussion have been carried out in step 2), help in time to clear up the microbial impurities that cleaning generates, to reach
To better experiment effect.The Candle-sticks stinkbug nymph after hatching obtained in step 4) is subjected to laboratory conventinal breeding later.
In some embodiments, the laboratory conventinal breeding method includes feeding distilled water and tussah chrysalis and/or glues
Worm.In step 4) by Candle-sticks stinkbug ovum it is smooth one down be placed on 12cm sterilizing glass culture dish in.It should be noted that Candle-sticks stinkbug
The oophyte shape of ovum is oval structures, and one end end face border of Candle-sticks stinkbug ovum is with outside small burr, a Duan Duan on the other side
Face is smooth, does not in addition grow other structures, and there is the opening of the broken ovum of hatching in long jagged one end end face, and Candle-sticks stinkbug nymph opens
Egg apparatus can puncture spinule, and that irises out, therefore smooth face-down, facilitates the hatching of Candle-sticks stinkbug ovum, the hatching of ovum can be improved
Rate.The oscillator of step 2) is turbula shaker.Turbula shaker is easily operated and regulates and controls, and certainly, the application is not limited to be vortexed
Oscillator；The tool that reagent in centrifuge tube is sucked out step 2) is pipette, and pipette is easily operated and regulates and controls, certainly, this
Application is not limited to pipette.
In one or more embodiments, centrifuge tube is put into time that oscillator shakes in step 2) is 15s,
After test of many times, duration of oscillation it is longer or it is shorter be not well positioned to meet mixing disinfection needs, duration of oscillation be the 15s time
Preferably；Preferably, the revolving speed of oscillator is 700~800rpm in step 2), and after test of many times, oscillation intensity is smaller or mistake
It is not well positioned to meet the needs of mixing cleaning mostly, oscillation revolving speed is that 700~800rpm time is preferable.
In at least one embodiment, the photoperiod of the environment of culture Candle-sticks stinkbug ovum is 16L:8D, the light of oophyte in step 4)
It is extremely important to oophyte development according to the period, it is obtained through test of many times, the photoperiod is within the scope of 15L:9D~17L:7D, and Candle-sticks stinkbug incubates
Rate is higher, and the photoperiod be 16L:8D when, hatching rate is best.
The Candle-sticks stinkbug ovum handled by above-mentioned experimental procedure, after hatching forms adult, under the normal husbandry conditions of laboratory,
Egg laying amount is effectively increased, so that the expansion for improving Candle-sticks stinkbug population is numerous, saves economy, time and the cost of labor of the raising of Candle-sticks stinkbug.
It is illustrated below by way of specific embodiment:
Embodiment 1
A method of extending Candle-sticks stinkbug Life of Adult, includes the following steps: 1) to be put into 3~5 grams of new Candle-sticks stinkbug ovum that produce
In 50ml centrifuge tube；2) after the sterile deionized water for not crossing Candle-sticks stinkbug ovum is added into the centrifuge tube in step 1), centrifuge tube is put
Enter to carry out the processing of concussion three times in oscillator, is every time that 700~800rpm shakes 15s with revolving speed, it is adjacent to shake processing twice
Between stand centrifuge tube 3min；Then the sterile deionized water in centrifuge tube is sucked out；It is added again to centrifuge tube and does not cross Candle-sticks stinkbug ovum
For volumetric concentration centrifuge tube to be put into oscillator and carries out 15s after 75% ethanol solution, revolving speed is the concussion of 700~800rpm
After standing 4min, then the ethanol solution in the centrifuge tube is sucked out for processing；3) by Candle-sticks stinkbug ovum obtained in step 2) with going out
After bacterium deionized water is rinsed completely, then Candle-sticks stinkbug oophyte is placed in sterilizing filter paper and blots surface moisture；4) by step 3)
Obtained in Candle-sticks stinkbug ovum be uniformly placed in 12cm sterilizes culture dish, be arranged culture environment temperature be 25~27 DEG C, humidity
For 60~70%, photoperiod 16L:6D；The sterilizing filter paper for having sprayed sterile purified water is placed in the culture dish, daily to
A sterile purified water is sprayed in culture dish, until Candle-sticks stinkbug egg hatching.Here setting is set as by the processed oophyte of the above method
EG group.
Embodiment 2
A method of extending Candle-sticks stinkbug Life of Adult, includes the following steps: 1) to be put into 3~5 grams of new Candle-sticks stinkbug ovum that produce
In 50ml centrifuge tube；2) after the sterile deionized water for not crossing Candle-sticks stinkbug ovum is added into the centrifuge tube in step 1), centrifuge tube is put
Enter to carry out four concussions processing in oscillator, is every time that 700~800rpm shakes 15s with revolving speed, it is adjacent to shake processing twice
Between stand centrifuge tube 3min；Then the sterile deionized water in centrifuge tube is sucked out；3) Candle-sticks stinkbug ovum obtained in step 2) is used
After sterile deionized water is rinsed completely, then Candle-sticks stinkbug oophyte is placed in sterilizing filter paper and blots surface moisture；4) by step
3) Candle-sticks stinkbug ovum obtained in is uniformly placed in 12cm sterilizes culture dish, be arranged culture environment temperature be 25~27 DEG C, it is wet
Degree is 60~70%, photoperiod 16L:6D；The sterilizing filter paper for having sprayed sterile purified water is placed in the culture dish, daily to
A sterile purified water is sprayed in culture dish, until Candle-sticks stinkbug egg hatching.Here setting is set as by the processed oophyte of the above method
PG group.
Comparative example
Untreated new production Candle-sticks stinkbug 3~5g of ovum is taken, is uniformly placed in 12cm sterilizes culture dish, culture environment is set
Temperature be 25~27 DEG C, humidity is 60~70%, photoperiod 16L:6D；It is placed in the culture dish and has sprayed aseptic distillation
The sterilizing filter paper of water sprays a sterile purified water into culture dish daily, until Candle-sticks stinkbug egg hatching.Here above-mentioned side is passed through in setting
The processed oophyte of method is set as CG group.
A sterile purified water is sprayed to EG group, PG group and CG group culture dish daily, until egg hatching, if the Candle-sticks stinkbug after hatching
Worm and adult carry out conventinal breeding in laboratory, and conventinal breeding method includes providing distilled water and tussah chrysalis, distilled water to Candle-sticks stinkbug
Add primary, replacement in tussah chrysalis 3~5 days daily once；The raising of nymph single head, the processes such as male pairing raising of adult one female one, due to
Conventinal breeding method is well-known technique, is not discussed here.The experiment of embodiment 1, embodiment 2 and comparative example is respectively set
5 experiments repeat, and substantially avoid experimental implementation contingency, and it is right that each group respectively matches Candle-sticks stinkbug more than 400, randomly select 100 pairs of notes
Record its situation such as table 1 of laying eggs.
1 Candle-sticks stinkbug ovum of table Candle-sticks stinkbug Adult worms producting eggs situation statistical form after different disposal
As seen from Table 1: PG female adult service life 47.64d is increased relative to CG female adult service life 44.98d, but not significant,
EG female adult service life 54.39d ratio CG long 9d (F2,297=5.125, P=0.0065)；PG longevity of male 63.78d is relative to CG female adult
Service life, 67.01d decreased, but without significant difference, and EG longevity of male 76.49d is considerably longer than CG (long 9.5d) and PG is (long
13d) (F2,297=12.03, P < 0.0001)；And the egg-laying season 37.54d of EG is considerably longer than the 29.78d's and PG of CG
32.04d (F2,297=12.03, P < 0.0001).Prove that this method can extend the service life of Candle-sticks stinkbug adult well, moreover it is possible to effectively
Effective egg-laying season that ground extends Candle-sticks stinkbug is long (laying eggs for the first time the time once laid eggs to the end), equally also extends its pest controlling
Duration.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example
Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not
It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office
It can be combined in any suitable manner in one or more embodiment or examples.In addition, without conflicting with each other, the skill of this field
Art personnel can tie the feature of different embodiments or examples described in this specification and different embodiments or examples
It closes and combines.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of method for extending Candle-sticks stinkbug Life of Adult, which comprises the steps of:
1) the new Candle-sticks stinkbug ovum that produces is put into centrifuge tube；
2) after sterile deionized water being added into the centrifuge tube in step 1), the centrifuge tube is put into oscillator and is carried out
At least concussion is handled three times；2.5~3.5min of centrifuge tube is stood between the adjacent concussion processing described twice；Then by the centrifugation
Sterile deionized water in pipe is sucked out；
3) after being rinsed Candle-sticks stinkbug ovum obtained in step 2) completely with sterile deionized water, Candle-sticks stinkbug ovum is blotted with sterilizing filter paper
Surface moisture.
2. a kind of method for extending Candle-sticks stinkbug Life of Adult according to claim 1, which is characterized in that newly produced described in step 1)
The volume of Candle-sticks stinkbug ovum accounts for the 1/3~2/5 of centrifugation pipe volume；In step 2), no mistake is added into the centrifuge tube in step 1)
After the sterile deionized water of Candle-sticks stinkbug ovum, centrifuge tube is put into oscillator and is at least shaken processing three times, is with revolving speed every time
500-1000rpm shakes 13~17s, stands 2.5~3.5min of centrifuge tube between the adjacent processing of concussion twice.
3. a kind of method for extending Candle-sticks stinkbug Life of Adult according to claim 2, which is characterized in that, will be described in step 2)
After sterile deionized water in centrifuge tube is sucked out, then it is added to the centrifuge tube in step 2) and does not cross the volumetric concentration of Candle-sticks stinkbug ovum and be
After 4% formalin, centrifuge tube is put into 13~17s of progress in oscillator, the concussion that revolving speed is 500-1000rpm is handled, quiet
After setting 4~5min, the formalin in the centrifuge tube is sucked out.
4. a kind of method for extending Candle-sticks stinkbug Life of Adult according to claim 2, which is characterized in that, will be described in step 2)
After sterile deionized water in centrifuge tube is sucked out, then it is added to the centrifuge tube in step 2) and does not cross the volumetric concentration of Candle-sticks stinkbug ovum and be
After 75% ethanol solution, centrifuge tube is put into 13~17s of progress in oscillator, the concussion that revolving speed is 500-1000rpm is handled, quiet
After setting 4~5min, the ethanol solution in the centrifuge tube is sucked out.
5. a kind of method for extending Candle-sticks stinkbug Life of Adult according to claim 1, which is characterized in that in step 3), by step
2) after Candle-sticks stinkbug ovum obtained in is rinsed completely with sterile deionized water, then Candle-sticks stinkbug oophyte is placed in sterilizing filter paper and is blotted
Surface moisture.
6. a kind of method for extending Candle-sticks stinkbug Life of Adult according to claim 1, which is characterized in that the extension Candle-sticks stinkbug adult
The method in service life further includes step 4), and Candle-sticks stinkbug ovum obtained in step 3) is uniformly placed in sterilizes culture dish, setting training
The temperature for supporting environment is 25~27 DEG C, humidity is 60~70%, the photoperiod is 15L:9D~17L:7D；It is placed in the culture dish
The sterilizing filter paper of sterile purified water has been sprayed, has sprayed a sterile purified water into culture dish daily, until Candle-sticks stinkbug egg hatching.
7. a kind of method for extending Candle-sticks stinkbug Life of Adult according to claim 6, which is characterized in that will be obtained in step 4)
Candle-sticks stinkbug nymph after hatching carries out laboratory conventinal breeding, and the laboratory conventinal breeding method includes feeding distilled water and tussah
Pupa and/or mythimna separata.
8. a kind of method for extending Candle-sticks stinkbug Life of Adult according to claim 6, which is characterized in that cultivate Candle-sticks stinkbug in step 4)
The photoperiod of the environment of ovum is 16L:8D.
9. a kind of method for extending Candle-sticks stinkbug Life of Adult according to claim 1, which is characterized in that the oscillation of step 2)
Device is turbula shaker；The tool that reagent in the centrifuge tube is sucked out step 2) is pipette.
10. a kind of method for extending Candle-sticks stinkbug Life of Adult according to claim 1, which is characterized in that step 1) is by 3~5 grams
The new Candle-sticks stinkbug ovum that produces is put into 50ml centrifuge tube；Centrifuge tube is put into the time that oscillator shakes as 15s in step 2)；Step
It is rapid 2) described in oscillator revolving speed be 700~800rpm.
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| CN109757441A (en) * | 2019-03-13 | 2019-05-17 | 中国农业科学院植物保护研究所 | A method of storage Candle-sticks stinkbug |
| CN110447604A (en) * | 2019-09-17 | 2019-11-15 | 中国农业科学院植物保护研究所 | A method of addition Hosts plant improves Candle-sticks stinkbug feeding effect |
| CN110476901A (en) * | 2019-09-17 | 2019-11-22 | 中国农业科学院植物保护研究所 | A method of extending Candle-sticks stinkbug ovum shelf life |
| CN114521537A (en) * | 2022-02-17 | 2022-05-24 | 中国农业科学院植物保护研究所 | Lugworm diapause induction method and long-term storage method |
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